CmoCh04G012970 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G012970
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionSerine/arginine-rich splicing factor RS2Z32
LocationCmo_Chr04: 6591296 .. 6593106 (+)
RNA-Seq ExpressionCmoCh04G012970
SyntenyCmoCh04G012970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAATCGAACACTAAACGCACTAACTTTCTCACACCGGAATCAGCCGATCGGAAGTCGATTGAAGAAACCCGGGAACAAAAATTCGAGGAGATCAGAACGAAAAAAAAAAAGAAAAAAACTAAAACAGCACAATAACAAAACAAACTATTTCCTAATTGCAAAACGATTAGAAAGTATGAGAATCTCGAAGAATTCCAAATGCAATCAGTCCAAAGCACACATAAAAGGGATGGATTCGAACTGAAGTTTTATCGGAGAGGGAAAGAGATTAAGAAAAGCGTCGGAAACCGAGGGAAATCTTTGGAAGTGGAGGGGGATGATTATTTTTAGTTTGGTATTCTTTTGCCCTTTTCCGTTTTTCTCTTGCAAAATTTTTCTTTCCCGTCTTAAAACGCATTGACTATTTTATTTATACGAACAGTTGTATCAGTCAAATTGCATTTTCGCTTTTTATTTTTTATTTTGTGTATTAAATTATACAAAATCGCCCCTAGGTTTTTATTATGTCCTAAATTTGGAAATGTTTTTATTAAAATTAAAAAAAACCACCTTCCAATTTGTTTTTTTTAATTATATATAATTATATATTTTTTAGTTCAACCATATACAAGTAATTTATTTCATGACATATTAAACCGCTAAATAAATAAGTAAGAAAGTACCAAATCTAGACCCATTTGGATTTTAATTTTTAATTTTTAATTTTTGAAAATTAAATTTATAACATAAAATCCATGTCAAAGTTTTAAACCTAAAAATACAAGAATACCAAATAGTTTTCTATTGGGCTTAAAAGTAGCCCAATCAACCTGTTTTAGTCGGCGCGGCCCACAACAATTGAGCCCACAACAATTGTTATCTGTAAAATTGGGCCTACGGCTGTTCCATAAAAAGAAATCGGTATAAAAATATCTGGAGAGGCTTACGGAAATCATTTTTTTTCATGCTGAAGACGGGAAATTTAGGGTTTCGTATTCTATCCAGGTGCGCCTCCTTCGAATTCTCTAGGTTTGCTCTCCTCGAAGCATTCGGGTCAAAATTCATAGCATATTTCTATTGATTTTTTTTTTTGAGCCCAATTAAGGTTTTGTATAGTCTCTATTTAGATTAAACAAATAGTTACTGGTTTGTTTATACTGTCAGTGATTGTGGTGGTACTGCGATTGTCAATTGTAGATAAAATGCCAAGGTACGACGATCGTTACGGTGGTACTCGGCTCTACGTTGGGCGCCTGTCTTCACGGACGAGGTCCCGTGATCTGGATGACTTGTTCAGTCGTTATGGAAGGTATGCAGAATCTAGCCGGATGTACCGTACCGTTCAGCCTTGGTCGAATTTGTGTGCTAACATTAATTTGTTGAATTCGATAAGTGTATGTGTGTTTAGCTATGCTGTATTTTACAGATGTAGATTCTAGCCGGATATACTGTTCATCCTTGGTTGAATTTGTGTGCTAACATTAATTTGTTGAATTCGAAAGGTGTATGTGTGTTTAGTTATTCTTCGCTTACTTCATTTGTCCCGGTTATGCTATTGCCAACATTATTATCAGACCTCTTGAGGCATTTACCAAGCCCTTTTTTTGGGTGCACGACTGTAAATGTGTATTTTGATATATCCGTGAAAGTGTGGATTGTTGATGGGTCTGTGTGGTTTAACTTGAGTGATCTTGTTGAAGGTTTTTTGGCTTATGCCAAACCTGGAGGTCTATGGTGAGAGGGTTCCCTTGGTGGATTTGGCGAGTCTTTCTATTATGTTATTGCCTGTTTTTATGGAAATGCCAAGAATCTCAGAGTGGTGTTTAG

mRNA sequence

ATGAACAATCGAACACTAAACGCACTAACTTTCTCACACCGGAATCAGCCGATCGGAAGTCGATTGAAGAAACCCGGGAACAAAAATTCGAGGAGATCAGAACGAAAAAAAAAAAGAAAAAAACTAAAACAGCACAATAACAAAACAAACTATTTCCTAATTGCAAAACGATTAGAAAGTGCGCCTCCTTCGAATTCTCTAGATAAAATGCCAAGGTACGACGATCGTTACGGTGGTACTCGGCTCTACGTTGGGCGCCTGTCTTCACGGACGAGGTCCCGTGATCTGGATGACTTGTTCAGTCGTTATGGAAGGTTTTTTGGCTTATGCCAAACCTGGAGGTCTATGGTGAGAGGGTTCCCTTGGTGGATTTGGCGAGTCTTTCTATTATGTTATTGCCTGTTTTTATGGAAATGCCAAGAATCTCAGAGTGGTGTTTAG

Coding sequence (CDS)

ATGAACAATCGAACACTAAACGCACTAACTTTCTCACACCGGAATCAGCCGATCGGAAGTCGATTGAAGAAACCCGGGAACAAAAATTCGAGGAGATCAGAACGAAAAAAAAAAAGAAAAAAACTAAAACAGCACAATAACAAAACAAACTATTTCCTAATTGCAAAACGATTAGAAAGTGCGCCTCCTTCGAATTCTCTAGATAAAATGCCAAGGTACGACGATCGTTACGGTGGTACTCGGCTCTACGTTGGGCGCCTGTCTTCACGGACGAGGTCCCGTGATCTGGATGACTTGTTCAGTCGTTATGGAAGGTTTTTTGGCTTATGCCAAACCTGGAGGTCTATGGTGAGAGGGTTCCCTTGGTGGATTTGGCGAGTCTTTCTATTATGTTATTGCCTGTTTTTATGGAAATGCCAAGAATCTCAGAGTGGTGTTTAG

Protein sequence

MNNRTLNALTFSHRNQPIGSRLKKPGNKNSRRSERKKKRKKLKQHNNKTNYFLIAKRLESAPPSNSLDKMPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWWIWRVFLLCYCLFLWKCQESQSGV
Homology
BLAST of CmoCh04G012970 vs. ExPASy Swiss-Prot
Match: Q9FYB7 (Serine/arginine-rich splicing factor RS2Z32 OS=Arabidopsis thaliana OX=3702 GN=RS2Z32 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.8e-11
Identity = 32/36 (88.89%), Postives = 34/36 (94.44%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGR 106
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGR
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGR 36

BLAST of CmoCh04G012970 vs. ExPASy Swiss-Prot
Match: Q8VYA5 (Serine/arginine-rich splicing factor RS2Z33 OS=Arabidopsis thaliana OX=3702 GN=RS2Z33 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.8e-11
Identity = 32/36 (88.89%), Postives = 34/36 (94.44%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGR 106
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGR
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGR 36

BLAST of CmoCh04G012970 vs. ExPASy TrEMBL
Match: A0A7J6GY79 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_027092 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.6e-23
Identity = 52/60 (86.67%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWWIWRVFL 129
           MPRYDDRYGGTRLYVGRL SR R RDL+DLFSRYGRFFG CQTWRS+VRG  WWIWRVFL
Sbjct: 1   MPRYDDRYGGTRLYVGRLPSRVRGRDLEDLFSRYGRFFGFCQTWRSVVRGSHWWIWRVFL 60

BLAST of CmoCh04G012970 vs. ExPASy TrEMBL
Match: D7TQD0 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_08s0040g02850 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 4.6e-18
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWW 124
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGRF G C TWRSMV G+ WW
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLESLFSRYGRFLGFCLTWRSMVGGWNWW 54

BLAST of CmoCh04G012970 vs. ExPASy TrEMBL
Match: M1HA94 (Arginine/serine-rich splicing factor RS2Z39 transcript V OS=Zea mays OX=4577 GN=RS2Z39 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 7.4e-16
Identity = 42/56 (75.00%), Postives = 47/56 (83.93%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWWIW 126
           MPRYDDRYGGTRLYVGRL++RTRSRDL+ LF RYGRFFG  +  RS+V G PWW W
Sbjct: 1   MPRYDDRYGGTRLYVGRLATRTRSRDLEHLFGRYGRFFGFYR--RSLVEGCPWWTW 54

BLAST of CmoCh04G012970 vs. ExPASy TrEMBL
Match: A0A7G2EX24 ((thale cress) hypothetical protein OS=Arabidopsis thaliana OX=3702 GN=AT9943_LOCUS13524 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 8.2e-15
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGF 121
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGRF G+C TWR ++ G+
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGRFLGVCLTWRCLMNGW 51

BLAST of CmoCh04G012970 vs. ExPASy TrEMBL
Match: A0A061ERP0 (RNA-binding family protein with retrovirus zinc finger-like domain, putative OS=Theobroma cacao OX=3641 GN=TCM_022039 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.4e-14
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRG 120
           MPR+D RYG TRLYVGRLSSRTR+RDL+ +FSRYGRF G C TWR MVRG
Sbjct: 1   MPRFDGRYGTTRLYVGRLSSRTRARDLEHIFSRYGRFLGFCLTWRPMVRG 50

BLAST of CmoCh04G012970 vs. NCBI nr
Match: KAF4372419.1 (hypothetical protein F8388_027092 [Cannabis sativa] >KAF4387805.1 hypothetical protein G4B88_004132 [Cannabis sativa] >KAF4399726.1 hypothetical protein G4B88_022809 [Cannabis sativa])

HSP 1 Score: 119.0 bits (297), Expect = 3.4e-23
Identity = 52/60 (86.67%), Postives = 54/60 (90.00%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWWIWRVFL 129
           MPRYDDRYGGTRLYVGRL SR R RDL+DLFSRYGRFFG CQTWRS+VRG  WWIWRVFL
Sbjct: 1   MPRYDDRYGGTRLYVGRLPSRVRGRDLEDLFSRYGRFFGFCQTWRSVVRGSHWWIWRVFL 60

BLAST of CmoCh04G012970 vs. NCBI nr
Match: KAG6669979.1 (hypothetical protein CIPAW_01G279300 [Carya illinoinensis])

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-18
Identity = 47/50 (94.00%), Postives = 47/50 (94.00%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRG 120
           MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRF G C TWRSMVRG
Sbjct: 1   MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFLGFCLTWRSMVRG 50

BLAST of CmoCh04G012970 vs. NCBI nr
Match: CBI32650.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 100.9 bits (250), Expect = 9.5e-18
Identity = 44/54 (81.48%), Postives = 47/54 (87.04%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWW 124
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGRF G C TWRSMV G+ WW
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLESLFSRYGRFLGFCLTWRSMVGGWNWW 54

BLAST of CmoCh04G012970 vs. NCBI nr
Match: KAF3972588.1 (hypothetical protein CMV_003911 [Castanea mollissima])

HSP 1 Score: 93.6 bits (231), Expect = 1.5e-15
Identity = 41/46 (89.13%), Postives = 43/46 (93.48%), Query Frame = 0

Query: 69  KMPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWR 115
           KMPRYDDRYGG+RLYVGRLSSRTRSRDLDD+FSRYGRF G C TWR
Sbjct: 528 KMPRYDDRYGGSRLYVGRLSSRTRSRDLDDIFSRYGRFLGFCLTWR 573

BLAST of CmoCh04G012970 vs. NCBI nr
Match: AGE46047.1 (arginine/serine-rich splicing factor RS2Z39 transcript V [Zea mays])

HSP 1 Score: 93.6 bits (231), Expect = 1.5e-15
Identity = 42/56 (75.00%), Postives = 47/56 (83.93%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGRFFGLCQTWRSMVRGFPWWIW 126
           MPRYDDRYGGTRLYVGRL++RTRSRDL+ LF RYGRFFG  +  RS+V G PWW W
Sbjct: 1   MPRYDDRYGGTRLYVGRLATRTRSRDLEHLFGRYGRFFGFYR--RSLVEGCPWWTW 54

BLAST of CmoCh04G012970 vs. TAIR 10
Match: AT2G37340.1 (arginine/serine-rich zinc knuckle-containing protein 33 )

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-12
Identity = 32/36 (88.89%), Postives = 34/36 (94.44%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGR 106
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGR
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGR 36

BLAST of CmoCh04G012970 vs. TAIR 10
Match: AT3G53500.2 (RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like domain )

HSP 1 Score: 70.5 bits (171), Expect = 1.3e-12
Identity = 32/36 (88.89%), Postives = 34/36 (94.44%), Query Frame = 0

Query: 70  MPRYDDRYGGTRLYVGRLSSRTRSRDLDDLFSRYGR 106
           MPRYDDRYG TRLYVGRLSSRTR+RDL+ LFSRYGR
Sbjct: 1   MPRYDDRYGNTRLYVGRLSSRTRTRDLERLFSRYGR 36

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYB71.8e-1188.89Serine/arginine-rich splicing factor RS2Z32 OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Q8VYA51.8e-1188.89Serine/arginine-rich splicing factor RS2Z33 OS=Arabidopsis thaliana OX=3702 GN=R... [more]
Match NameE-valueIdentityDescription
A0A7J6GY791.6e-2386.67Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_027092 PE=4 SV=1[more]
D7TQD04.6e-1881.48Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_08s0040g02850 PE=4 SV=... [more]
M1HA947.4e-1675.00Arginine/serine-rich splicing factor RS2Z39 transcript V OS=Zea mays OX=4577 GN=... [more]
A0A7G2EX248.2e-1576.47(thale cress) hypothetical protein OS=Arabidopsis thaliana OX=3702 GN=AT9943_LOC... [more]
A0A061ERP02.4e-1478.00RNA-binding family protein with retrovirus zinc finger-like domain, putative OS=... [more]
Match NameE-valueIdentityDescription
KAF4372419.13.4e-2386.67hypothetical protein F8388_027092 [Cannabis sativa] >KAF4387805.1 hypothetical p... [more]
KAG6669979.11.1e-1894.00hypothetical protein CIPAW_01G279300 [Carya illinoinensis][more]
CBI32650.39.5e-1881.48unnamed protein product, partial [Vitis vinifera][more]
KAF3972588.11.5e-1589.13hypothetical protein CMV_003911 [Castanea mollissima][more]
AGE46047.11.5e-1575.00arginine/serine-rich splicing factor RS2Z39 transcript V [Zea mays][more]
Match NameE-valueIdentityDescription
AT2G37340.11.3e-1288.89arginine/serine-rich zinc knuckle-containing protein 33 [more]
AT3G53500.21.3e-1288.89RNA-binding (RRM/RBD/RNP motifs) family protein with retrovirus zinc finger-like... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 14..109
e-value: 4.1E-6
score: 28.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 70..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G012970.1CmoCh04G012970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016607 nuclear speck
molecular_function GO:0140359 ABC-type transporter activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003729 mRNA binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003676 nucleic acid binding