CmoCh04G012870 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G012870
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionDirigent protein
LocationCmo_Chr04: 6525500 .. 6526237 (-)
RNA-Seq ExpressionCmoCh04G012870
SyntenyCmoCh04G012870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACCTCCATTTTGATTCTTTACTAACTTATTTTCTCTCGTTTTTGGCCATCGTTAACCTCTCGTCCTCCACTCGGACCTTCGAAAACCCAGTTCGAAACCGCCACCATAGCCATCATAAGCTTTCGTTTTTAATGAGAGATGTGTTCGATTCAACTTCTCGCTCCTCTACGTCCAAGTTCCCAGCAAACACCAAAACAACGACTAATCAATTACCATTTTCAAAACCCTTAGGGTTTTTTCTACCCAGGGGAGGAATTCCTATACCTGAAGTCTACACCACGACGACGACGACATCTCCTACTACTGGAATCTCTGCTCAAACACCTGATCTATCAACAATTGGAATATCGTTTCCTGTTAGGGCGATTCTCCAAGAGTTAGAGTACGGAATTGTGAGAGAGATTGATGAAGAGTTGTTCAAAACTTCGAGTCGTAAACCACATGTAGTTGTTGGGAGAATAAAAGGGTTGTACGTAGAAAACAACGGAAATAGTGGTGAGCCTATGATAGCTATGAGGGTGTATCTTGGTAACGGTGAGATTAAAGATGGGTTAAAAATGTTTGGCGTGTATAAAAGTAATCACGTGAAAGAGTCTCATATTACTATAATCGGTGGCATGGGCAAGTACCTTGGCGCTAATGGATATGCAACTTTGAAGAAATCATTTAGAACAAAGTTGGGAGCAAATTATAACAAGTTCATGGAGTTGAATGTTTACTTGAGCAAATAG

mRNA sequence

ATGGCCAACCTCCATTTTGATTCTTTACTAACTTATTTTCTCTCGTTTTTGGCCATCGTTAACCTCTCGTCCTCCACTCGGACCTTCGAAAACCCAGTTCGAAACCGCCACCATAGCCATCATAAGCTTTCGTTTTTAATGAGAGATGTGTTCGATTCAACTTCTCGCTCCTCTACGTCCAAGTTCCCAGCAAACACCAAAACAACGACTAATCAATTACCATTTTCAAAACCCTTAGGGTTTTTTCTACCCAGGGGAGGAATTCCTATACCTGAAGTCTACACCACGACGACGACGACATCTCCTACTACTGGAATCTCTGCTCAAACACCTGATCTATCAACAATTGGAATATCGTTTCCTGTTAGGGCGATTCTCCAAGAGTTAGAGTACGGAATTGTGAGAGAGATTGATGAAGAGTTGTTCAAAACTTCGAGTCGTAAACCACATGTAGTTGTTGGGAGAATAAAAGGGTTGTACGTAGAAAACAACGGAAATAGTGGTGAGCCTATGATAGCTATGAGGGTGTATCTTGGTAACGGTGAGATTAAAGATGGGTTAAAAATGTTTGGCGTGTATAAAAGTAATCACGTGAAAGAGTCTCATATTACTATAATCGGTGGCATGGGCAAGTACCTTGGCGCTAATGGATATGCAACTTTGAAGAAATCATTTAGAACAAAGTTGGGAGCAAATTATAACAAGTTCATGGAGTTGAATGTTTACTTGAGCAAATAG

Coding sequence (CDS)

ATGGCCAACCTCCATTTTGATTCTTTACTAACTTATTTTCTCTCGTTTTTGGCCATCGTTAACCTCTCGTCCTCCACTCGGACCTTCGAAAACCCAGTTCGAAACCGCCACCATAGCCATCATAAGCTTTCGTTTTTAATGAGAGATGTGTTCGATTCAACTTCTCGCTCCTCTACGTCCAAGTTCCCAGCAAACACCAAAACAACGACTAATCAATTACCATTTTCAAAACCCTTAGGGTTTTTTCTACCCAGGGGAGGAATTCCTATACCTGAAGTCTACACCACGACGACGACGACATCTCCTACTACTGGAATCTCTGCTCAAACACCTGATCTATCAACAATTGGAATATCGTTTCCTGTTAGGGCGATTCTCCAAGAGTTAGAGTACGGAATTGTGAGAGAGATTGATGAAGAGTTGTTCAAAACTTCGAGTCGTAAACCACATGTAGTTGTTGGGAGAATAAAAGGGTTGTACGTAGAAAACAACGGAAATAGTGGTGAGCCTATGATAGCTATGAGGGTGTATCTTGGTAACGGTGAGATTAAAGATGGGTTAAAAATGTTTGGCGTGTATAAAAGTAATCACGTGAAAGAGTCTCATATTACTATAATCGGTGGCATGGGCAAGTACCTTGGCGCTAATGGATATGCAACTTTGAAGAAATCATTTAGAACAAAGTTGGGAGCAAATTATAACAAGTTCATGGAGTTGAATGTTTACTTGAGCAAATAG

Protein sequence

MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELNVYLSK
Homology
BLAST of CmoCh04G012870 vs. ExPASy Swiss-Prot
Match: Q9T0H8 (Dirigent protein 18 OS=Arabidopsis thaliana OX=3702 GN=DIR18 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.3e-09
Identity = 42/158 (26.58%), Postives = 73/158 (46.20%), Query Frame = 0

Query: 72  QLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI-------GISFPVRA 131
           Q+PF+K +GF  P+ G+ IP       T +   GI   T    T        GI   +  
Sbjct: 64  QVPFAKQIGFVPPQNGVAIPNANGAMPTVNGINGIPLGTGLSGTAFSGQNLNGIQTQLGP 123

Query: 132 ILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIK 191
               L +G +  ID ++  +        +G+ +G+YV ++ +    M+A    L  GE  
Sbjct: 124 DGLSLGFGTITVID-DIITSGPDLGSQPLGKAQGVYVASSADGSTQMMAFTAMLEGGEYN 183

Query: 192 DGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLK 223
           D L  +G+Y+      SH+++ GG G++  A G+A ++
Sbjct: 184 DNLNFYGIYRIGSAM-SHLSVTGGTGRFKNACGFAEVR 219

BLAST of CmoCh04G012870 vs. ExPASy Swiss-Prot
Match: Q7Y225 (Dirigent protein 16 OS=Arabidopsis thaliana OX=3702 GN=DIR16 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-08
Identity = 42/158 (26.58%), Postives = 72/158 (45.57%), Query Frame = 0

Query: 72  QLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI-------GISFPVRA 131
           Q+PF+K +GF  P  GI IP       T +   G+   T    T        GI   +  
Sbjct: 63  QVPFAKQIGFTPPENGIAIPNANGALPTVNGINGVPLGTGLSGTAYSGQNLNGIQTQLGP 122

Query: 132 ILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIK 191
               L +G +  ID ++  +        +G+ +G+YV ++ +    M+A    L  GE  
Sbjct: 123 DGLSLGFGTITVID-DILTSGPDLGSQPLGKAQGVYVASSADGSTQMMAFTAMLEGGEYN 182

Query: 192 DGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLK 223
           D L  +G+Y+      SH+++ GG G++  A G+A ++
Sbjct: 183 DNLNFYGIYRIGSAM-SHLSVTGGTGRFKNACGFAEVR 218

BLAST of CmoCh04G012870 vs. ExPASy Swiss-Prot
Match: Q9SIA8 (Dirigent protein 10 OS=Arabidopsis thaliana OX=3702 GN=DIR10 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.2e-07
Identity = 67/237 (28.27%), Postives = 103/237 (43.46%), Query Frame = 0

Query: 43  LSFLMRDVF---DSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLP-RGGIP--------- 102
           L F M D+    + T+R+ T          + QLPF+KP G  LP   G+P         
Sbjct: 218 LVFFMHDILGGSNPTARAVTG--VVANPALSGQLPFAKPNGANLPVSNGVPSNNNNNGIV 277

Query: 103 ----IPEVYTTTTTTSPTTGISAQTPDLSTIGISFPVR--------AILQELEYGIVREI 162
               +P +     TT+    I     + + I   FPV         + LQ L +G +  I
Sbjct: 278 NNNNVPFLVGLGGTTA---NILQNNNNGNNILNGFPVASGGQLPSGSALQMLMFGTMTVI 337

Query: 163 DEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIKDGLKMFGVYKSNH 222
           D+EL +        ++G+ +G YV +  +     +A      +G  +D +  FGV ++  
Sbjct: 338 DDELTE-GHELGSGLLGKAQGYYVASAIDGTSQTMAFTAMFESGGYEDSISFFGVLRT-A 397

Query: 223 VKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNK----------FMELNVYLS 245
           V ESHI ++GG GKY+ A G+A L K+F    G   N+           +E  VYLS
Sbjct: 398 VSESHIGVMGGTGKYVNARGFAIL-KTFTGSSGTQQNQPHQFTDGLETVVECTVYLS 446

BLAST of CmoCh04G012870 vs. ExPASy Swiss-Prot
Match: O80630 (Dirigent protein 9 OS=Arabidopsis thaliana OX=3702 GN=DIR9 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-07
Identity = 61/238 (25.63%), Postives = 100/238 (42.02%), Query Frame = 0

Query: 43  LSFLMRDVFDSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLP-RGGIPIPEVYTTTTTTS 102
           L F M DV   +  S+        +T  N +PFSK      P   G+P+       +  +
Sbjct: 87  LEFFMHDVLGGSHPSARVVTGIVAQTEVNGIPFSKASNSIFPVDNGVPLVNSNNINSVIN 146

Query: 103 PTT-------------------------GISAQTPDLSTIGISFPVRAILQELEYGIVRE 162
           P T                          +SA +    T G + P  A LQ L +G +  
Sbjct: 147 PNTAPLLTGLGGAQTSTVIQNTNGNSNDALSANSLPFVTAG-NLPPGAALQHLMFGTITV 206

Query: 163 IDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGE------IKDGLKMF 222
           +D+EL + S      V+GR +G Y+ ++ +     +++ V L +GE      + D +  F
Sbjct: 207 VDDELTE-SHELGSAVIGRAQGFYLASSLDGTSQTLSLTVLL-HGEHDQHDTLDDAISFF 266

Query: 223 GVYK-SNHVKESHITIIGGMGKYLGANGYA---TLKKSFRTKLGANYNKFMELNVYLS 245
           GV++ ++H   S I +IGG GK+  A GYA   TL       +    +  +  +VYL+
Sbjct: 267 GVHRTASHA--SQIAVIGGTGKFEHAKGYAIVETLHNQDNQHITDGQDTILHFSVYLT 319

BLAST of CmoCh04G012870 vs. ExPASy Swiss-Prot
Match: Q9M3C8 (Dirigent protein 24 OS=Arabidopsis thaliana OX=3702 GN=DIR24 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.8e-06
Identity = 57/236 (24.15%), Postives = 100/236 (42.37%), Query Frame = 0

Query: 43  LSFLMRDVFDSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLP-RGGIPIPEVYTTTTTTS 102
           L F M DV   +  S+        +T  N +PFSK      P    +P+    +     +
Sbjct: 70  LEFFMHDVLGGSHPSARVVTGIVAQTEVNGIPFSKSSNNIFPVDNAVPLVNANSINNLIN 129

Query: 103 PTT--------GISAQT----------PDLSTIGISF------PVRAILQELEYGIVREI 162
           P T        G  A T            LS+  + F      P  A LQ+L +G +  +
Sbjct: 130 PNTAPLLTGLSGSQANTVIQNSNGNSQGSLSSNNLPFVTTGQLPPIAALQQLMFGSITVV 189

Query: 163 DEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYL-----GNGEIKDGLKMFGV 222
           D+EL +        ++GR +G Y+ ++ +     +++ V L      +  + D +  FGV
Sbjct: 190 DDELTE-GHELGSAIIGRAQGFYLASSLDGTSQTLSLTVLLHEDHDHHDTLDDAISFFGV 249

Query: 223 YK-SNHVKESHITIIGGMGKYLGANGYA---TLKKSFRTKLGANYNKFMELNVYLS 245
           ++ ++H   SHI ++GG G++  A GYA   TL       +   ++  +  +VYL+
Sbjct: 250 HRTASHA--SHIAVVGGTGRFEHAKGYAVVETLHNQEDQHVTDGHDTILHFSVYLT 302

BLAST of CmoCh04G012870 vs. ExPASy TrEMBL
Match: A0A6J1GYQ5 (Dirigent protein OS=Cucurbita moschata OX=3662 GN=LOC111458697 PE=3 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 9.5e-133
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60
           MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS
Sbjct: 1   MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60

Query: 61  KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF 120
           KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF
Sbjct: 61  KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF 120

Query: 121 PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN 180
           PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN
Sbjct: 121 PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN 180

Query: 181 GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN 240
           GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN
Sbjct: 181 GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN 240

Query: 241 VYLSK 246
           VYLSK
Sbjct: 241 VYLSK 245

BLAST of CmoCh04G012870 vs. ExPASy TrEMBL
Match: A0A6J1JP91 (Dirigent protein OS=Cucurbita maxima OX=3661 GN=LOC111487107 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.4e-115
Identity = 224/249 (89.96%), Postives = 229/249 (91.97%), Query Frame = 0

Query: 1   MANLHFDSL----LTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSR 60
           MANLHFDSL    L YFLSF  IVN SSSTRTFENPVRNRHHSHHKLSFLMRDVF+STSR
Sbjct: 1   MANLHFDSLLIKVLAYFLSFTIIVNFSSSTRTFENPVRNRHHSHHKLSFLMRDVFNSTSR 60

Query: 61  SSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI 120
           SSTSK  ANTKTTTNQLPFSKPLGFFLP  GIPIPEVY TTTTTSPTT ISAQT DLSTI
Sbjct: 61  SSTSKSLANTKTTTNQLPFSKPLGFFLPSRGIPIPEVY-TTTTTSPTTEISAQTSDLSTI 120

Query: 121 GISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRV 180
           GISFPVRAILQELEYGIVREIDEELF+TS+RK HVVVGRIKGLYVENN NSGEPMIAMRV
Sbjct: 121 GISFPVRAILQELEYGIVREIDEELFRTSNRKSHVVVGRIKGLYVENNENSGEPMIAMRV 180

Query: 181 YLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKF 240
           YLG GEIKDGLKMFGVYKSNHVKESHITIIGGMG YLGANGYATLKKSFRT+ GANYNKF
Sbjct: 181 YLGKGEIKDGLKMFGVYKSNHVKESHITIIGGMGMYLGANGYATLKKSFRTRFGANYNKF 240

Query: 241 MELNVYLSK 246
           +ELNVYLSK
Sbjct: 241 IELNVYLSK 248

BLAST of CmoCh04G012870 vs. ExPASy TrEMBL
Match: A0A6J1CCF7 (Dirigent protein OS=Momordica charantia OX=3673 GN=LOC111010220 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.8e-82
Identity = 169/249 (67.87%), Postives = 190/249 (76.31%), Query Frame = 0

Query: 1   MANLHFDSLLT----YFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSR 60
           MANLHF SLL       L F  I+NLSSS RTFENP RNRH +  KLSFLMRDV + T+ 
Sbjct: 1   MANLHFHSLLNLTIYVLLIFTTIINLSSSARTFENPTRNRHRNRRKLSFLMRDVLNVTTL 60

Query: 61  SSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI 120
             +SK P  T   T++LPFSKPLG F P GGIPIPE Y  TTTTSP T +S QT DLSTI
Sbjct: 61  HPSSKSP--TTKATSELPFSKPLGLFPPNGGIPIPETY-ATTTTSPVTNLSGQTLDLSTI 120

Query: 121 GISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRV 180
           GISFPVRA LQELEYG VREI+EELF+TS  K  VVVGRI+G YVEN  +SG  M+AM V
Sbjct: 121 GISFPVRATLQELEYGAVREINEELFETSRHKLQVVVGRIEGFYVENGEDSGGHMMAMTV 180

Query: 181 YLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKF 240
           Y G GE KDGL++FGVY+ +HVKESH+ IIGG+GKYLGANGYATLKK  R KLG NYNKF
Sbjct: 181 YFGKGEAKDGLRLFGVYRGDHVKESHVAIIGGIGKYLGANGYATLKKLVRAKLGVNYNKF 240

Query: 241 MELNVYLSK 246
           +E +VYLSK
Sbjct: 241 IEFDVYLSK 246

BLAST of CmoCh04G012870 vs. ExPASy TrEMBL
Match: A0A5A7V959 (Dirigent protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00150 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 7.6e-74
Identity = 156/251 (62.15%), Postives = 184/251 (73.31%), Query Frame = 0

Query: 2   ANLHFDSLL-----TYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSR 61
           ANLH  SLL      + + F  I+ LSS+ RTFENP RNRH+ HHKLSF MRDVF+ST+ 
Sbjct: 3   ANLHSHSLLIKIIANFLIFFTTIITLSSAARTFENPTRNRHYRHHKLSFSMRDVFNSTTH 62

Query: 62  SSTSKFPANTKTTTN-QLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLST 121
             +   P  + T TN QLPFSKPL FF P  GIPI + Y TT  +      S+QTPD ST
Sbjct: 63  HYS---PTKSTTITNKQLPFSKPLDFFPPNKGIPISQTYPTTVGS-----FSSQTPDFST 122

Query: 122 IGISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENN-GNSGEPMIAM 181
           IGISFP R+ LQELEYG+VR IDEELF+ S  KPH ++GRI+G YVEN+  NSG  M+AM
Sbjct: 123 IGISFPSRSTLQELEYGMVRGIDEELFENSRSKPH-IIGRIEGFYVENSEENSGGHMMAM 182

Query: 182 RVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYN 241
            +Y G GE KDG+++FGVY+S+HVKESH+ IIGG GKY GANGYATLKKSFRTK G  YN
Sbjct: 183 TMYFGKGEAKDGIRVFGVYRSDHVKESHVAIIGGFGKYHGANGYATLKKSFRTKFGGKYN 242

Query: 242 KFMELNVYLSK 246
           KF+E NVYLSK
Sbjct: 243 KFIEFNVYLSK 244

BLAST of CmoCh04G012870 vs. ExPASy TrEMBL
Match: A0A0A0KNT3 (Dirigent protein OS=Cucumis sativus OX=3659 GN=Csa_5G179740 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 7.6e-74
Identity = 156/253 (61.66%), Postives = 185/253 (73.12%), Query Frame = 0

Query: 1   MANLHFD-SLL-----TYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDST 60
           MANLH+  SLL      + + F  I+ LSS+ RTFENP +NRH+ HH+LSF MRDVF+ST
Sbjct: 1   MANLHYSRSLLIKIIANFLIFFTTIITLSSAARTFENPTQNRHYRHHRLSFSMRDVFNST 60

Query: 61  SRSSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTG-ISAQTPDL 120
           +   +   P  +  T  QLPFSKPLGFF P  GIPI + Y       PTTG  S+QTPD 
Sbjct: 61  NHHYS---PTKSTMTNKQLPFSKPLGFFPPNQGIPISQTY-------PTTGSFSSQTPDF 120

Query: 121 STIGISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENN-GNSGEPMI 180
           STIGISFP R+ LQELEYG+VR IDEELF+ S  KPH V+GRI+G YVEN+  NSG  M+
Sbjct: 121 STIGISFPSRSTLQELEYGMVRGIDEELFEISKSKPH-VIGRIEGFYVENSEENSGGHMM 180

Query: 181 AMRVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGAN 240
            M +Y G GE KDG+++FGVY+S+HVKESH+ IIGG GKY GANGYATLKKSFRTK G  
Sbjct: 181 GMTMYFGKGEAKDGIRVFGVYRSDHVKESHVAIIGGFGKYHGANGYATLKKSFRTKFGGK 240

Query: 241 YNKFMELNVYLSK 246
           YNKF+E NVYLSK
Sbjct: 241 YNKFIEFNVYLSK 242

BLAST of CmoCh04G012870 vs. NCBI nr
Match: XP_022957256.1 (dirigent protein 9-like [Cucurbita moschata])

HSP 1 Score: 482.6 bits (1241), Expect = 2.0e-132
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0

Query: 1   MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60
           MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS
Sbjct: 1   MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60

Query: 61  KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF 120
           KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF
Sbjct: 61  KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTIGISF 120

Query: 121 PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN 180
           PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN
Sbjct: 121 PVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGN 180

Query: 181 GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN 240
           GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN
Sbjct: 181 GEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKFMELN 240

Query: 241 VYLSK 246
           VYLSK
Sbjct: 241 VYLSK 245

BLAST of CmoCh04G012870 vs. NCBI nr
Match: XP_022990120.1 (dirigent protein 10-like [Cucurbita maxima])

HSP 1 Score: 425.6 bits (1093), Expect = 2.8e-115
Identity = 224/249 (89.96%), Postives = 229/249 (91.97%), Query Frame = 0

Query: 1   MANLHFDSL----LTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSR 60
           MANLHFDSL    L YFLSF  IVN SSSTRTFENPVRNRHHSHHKLSFLMRDVF+STSR
Sbjct: 1   MANLHFDSLLIKVLAYFLSFTIIVNFSSSTRTFENPVRNRHHSHHKLSFLMRDVFNSTSR 60

Query: 61  SSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI 120
           SSTSK  ANTKTTTNQLPFSKPLGFFLP  GIPIPEVY TTTTTSPTT ISAQT DLSTI
Sbjct: 61  SSTSKSLANTKTTTNQLPFSKPLGFFLPSRGIPIPEVY-TTTTTSPTTEISAQTSDLSTI 120

Query: 121 GISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRV 180
           GISFPVRAILQELEYGIVREIDEELF+TS+RK HVVVGRIKGLYVENN NSGEPMIAMRV
Sbjct: 121 GISFPVRAILQELEYGIVREIDEELFRTSNRKSHVVVGRIKGLYVENNENSGEPMIAMRV 180

Query: 181 YLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNKF 240
           YLG GEIKDGLKMFGVYKSNHVKESHITIIGGMG YLGANGYATLKKSFRT+ GANYNKF
Sbjct: 181 YLGKGEIKDGLKMFGVYKSNHVKESHITIIGGMGMYLGANGYATLKKSFRTRFGANYNKF 240

Query: 241 MELNVYLSK 246
           +ELNVYLSK
Sbjct: 241 IELNVYLSK 248

BLAST of CmoCh04G012870 vs. NCBI nr
Match: KAG6601020.1 (Dirigent protein 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 422.5 bits (1085), Expect = 2.4e-114
Identity = 217/228 (95.18%), Postives = 220/228 (96.49%), Query Frame = 0

Query: 1   MANLHFDSLLTYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60
           MANLHFDSLLTYFL FLA+VNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS
Sbjct: 1   MANLHFDSLLTYFLLFLALVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSRSSTS 60

Query: 61  KFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVY-TTTTTTSPTTGISAQTPDLSTIGIS 120
           KFPANTKTT NQLPFSKPLGFFLP GGIPIPEVY TTTTTTSPTTG+SAQTPDLSTIGIS
Sbjct: 61  KFPANTKTTANQLPFSKPLGFFLPSGGIPIPEVYTTTTTTTSPTTGVSAQTPDLSTIGIS 120

Query: 121 FPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLG 180
           FPVRAILQELEYGIVREIDEELF TS RK HVVVGRIKGLYVENN NSGEPMIA+RVYLG
Sbjct: 121 FPVRAILQELEYGIVREIDEELFGTSRRKSHVVVGRIKGLYVENNENSGEPMIAIRVYLG 180

Query: 181 NGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRT 228
           NGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRT
Sbjct: 181 NGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRT 228

BLAST of CmoCh04G012870 vs. NCBI nr
Match: KAG7031632.1 (Dirigent protein 24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.0 bits (863), Expect = 1.3e-88
Identity = 173/182 (95.05%), Postives = 175/182 (96.15%), Query Frame = 0

Query: 47  MRDVFDSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVY-TTTTTTSPTTG 106
           MRDVFDSTSRSSTSKFPANTKTT NQLPFSKPLGFFLP GGIPIPEVY TTTTTTSPTTG
Sbjct: 1   MRDVFDSTSRSSTSKFPANTKTTANQLPFSKPLGFFLPSGGIPIPEVYTTTTTTTSPTTG 60

Query: 107 ISAQTPDLSTIGISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNG 166
           +SAQTPDLSTIGISFPVRAILQELEYGIVREIDEELF TS RK HVVVGRIKGLYVENN 
Sbjct: 61  VSAQTPDLSTIGISFPVRAILQELEYGIVREIDEELFGTSRRKSHVVVGRIKGLYVENNE 120

Query: 167 NSGEPMIAMRVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSF 226
           NSGEPMIA+RVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSF
Sbjct: 121 NSGEPMIAIRVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSF 180

Query: 227 RT 228
           RT
Sbjct: 181 RT 182

BLAST of CmoCh04G012870 vs. NCBI nr
Match: XP_038891666.1 (dirigent protein 10-like [Benincasa hispida])

HSP 1 Score: 318.9 bits (816), Expect = 3.7e-83
Identity = 166/250 (66.40%), Postives = 193/250 (77.20%), Query Frame = 0

Query: 1   MANLHFDSLL----TYFLSFLAIVNLSSSTRTFENPVRNRHHSHHKLSFLMRDVFDSTSR 60
           M  LHF SLL      FLSF  I+NLSSS R FENP RN H+ HHKLSFLMRDV +ST+ 
Sbjct: 1   MTKLHFHSLLMKTIACFLSFTTIINLSSSARIFENPTRNHHYRHHKLSFLMRDVLNSTTH 60

Query: 61  S-STSKFPANTKTTTNQLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLST 120
             S+SK P N  TT+ QLPFSKPLGFF P GGIPIPE YTTT +       S+QTPD+S+
Sbjct: 61  HYSSSKSPTNKATTSGQLPFSKPLGFFPPNGGIPIPETYTTTGS------FSSQTPDISS 120

Query: 121 IGISFPVRAILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMR 180
           IGISFP R  LQEL+YG+V+ IDEELF TS  K  VVVGRI+G YVEN+ +SG  M+A  
Sbjct: 121 IGISFPARTTLQELDYGMVKGIDEELFGTSRHKSRVVVGRIEGFYVENSEDSGGHMMATT 180

Query: 181 VYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNK 240
           +Y G GE+KDGL++FGVY+S+HVKESH+ IIGG+GKYLGANGYATLKKSFRTK GAN+N 
Sbjct: 181 MYFGKGEVKDGLRLFGVYRSDHVKESHVAIIGGLGKYLGANGYATLKKSFRTKFGANHNN 240

Query: 241 FMELNVYLSK 246
           F+E NVYLSK
Sbjct: 241 FIEFNVYLSK 244

BLAST of CmoCh04G012870 vs. TAIR 10
Match: AT4G13580.1 (Disease resistance-responsive (dirigent-like protein) family protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.9e-10
Identity = 42/158 (26.58%), Postives = 73/158 (46.20%), Query Frame = 0

Query: 72  QLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI-------GISFPVRA 131
           Q+PF+K +GF  P+ G+ IP       T +   GI   T    T        GI   +  
Sbjct: 64  QVPFAKQIGFVPPQNGVAIPNANGAMPTVNGINGIPLGTGLSGTAFSGQNLNGIQTQLGP 123

Query: 132 ILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIK 191
               L +G +  ID ++  +        +G+ +G+YV ++ +    M+A    L  GE  
Sbjct: 124 DGLSLGFGTITVID-DIITSGPDLGSQPLGKAQGVYVASSADGSTQMMAFTAMLEGGEYN 183

Query: 192 DGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLK 223
           D L  +G+Y+      SH+++ GG G++  A G+A ++
Sbjct: 184 DNLNFYGIYRIGSAM-SHLSVTGGTGRFKNACGFAEVR 219

BLAST of CmoCh04G012870 vs. TAIR 10
Match: AT3G24020.1 (Disease resistance-responsive (dirigent-like protein) family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.0e-09
Identity = 42/158 (26.58%), Postives = 72/158 (45.57%), Query Frame = 0

Query: 72  QLPFSKPLGFFLPRGGIPIPEVYTTTTTTSPTTGISAQTPDLSTI-------GISFPVRA 131
           Q+PF+K +GF  P  GI IP       T +   G+   T    T        GI   +  
Sbjct: 63  QVPFAKQIGFTPPENGIAIPNANGALPTVNGINGVPLGTGLSGTAYSGQNLNGIQTQLGP 122

Query: 132 ILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIK 191
               L +G +  ID ++  +        +G+ +G+YV ++ +    M+A    L  GE  
Sbjct: 123 DGLSLGFGTITVID-DILTSGPDLGSQPLGKAQGVYVASSADGSTQMMAFTAMLEGGEYN 182

Query: 192 DGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLK 223
           D L  +G+Y+      SH+++ GG G++  A G+A ++
Sbjct: 183 DNLNFYGIYRIGSAM-SHLSVTGGTGRFKNACGFAEVR 218

BLAST of CmoCh04G012870 vs. TAIR 10
Match: AT2G28670.1 (Disease resistance-responsive (dirigent-like protein) family protein )

HSP 1 Score: 58.5 bits (140), Expect = 8.5e-09
Identity = 67/237 (28.27%), Postives = 103/237 (43.46%), Query Frame = 0

Query: 43  LSFLMRDVF---DSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLP-RGGIP--------- 102
           L F M D+    + T+R+ T          + QLPF+KP G  LP   G+P         
Sbjct: 218 LVFFMHDILGGSNPTARAVTG--VVANPALSGQLPFAKPNGANLPVSNGVPSNNNNNGIV 277

Query: 103 ----IPEVYTTTTTTSPTTGISAQTPDLSTIGISFPVR--------AILQELEYGIVREI 162
               +P +     TT+    I     + + I   FPV         + LQ L +G +  I
Sbjct: 278 NNNNVPFLVGLGGTTA---NILQNNNNGNNILNGFPVASGGQLPSGSALQMLMFGTMTVI 337

Query: 163 DEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGEIKDGLKMFGVYKSNH 222
           D+EL +        ++G+ +G YV +  +     +A      +G  +D +  FGV ++  
Sbjct: 338 DDELTE-GHELGSGLLGKAQGYYVASAIDGTSQTMAFTAMFESGGYEDSISFFGVLRT-A 397

Query: 223 VKESHITIIGGMGKYLGANGYATLKKSFRTKLGANYNK----------FMELNVYLS 245
           V ESHI ++GG GKY+ A G+A L K+F    G   N+           +E  VYLS
Sbjct: 398 VSESHIGVMGGTGKYVNARGFAIL-KTFTGSSGTQQNQPHQFTDGLETVVECTVYLS 446

BLAST of CmoCh04G012870 vs. TAIR 10
Match: AT2G28670.2 (Disease resistance-responsive (dirigent-like protein) family protein )

HSP 1 Score: 58.5 bits (140), Expect = 8.5e-09
Identity = 60/207 (28.99%), Postives = 92/207 (44.44%), Query Frame = 0

Query: 70  TNQLPFSKPLGFFLP-RGGIP-------------IPEVYTTTTTTSPTTGISAQTPDLST 129
           + QLPF+KP G  LP   G+P             +P +     TT+    I     + + 
Sbjct: 25  SGQLPFAKPNGANLPVSNGVPSNNNNNGIVNNNNVPFLVGLGGTTA---NILQNNNNGNN 84

Query: 130 IGISFPVR--------AILQELEYGIVREIDEELFKTSSRKPHVVVGRIKGLYVENNGNS 189
           I   FPV         + LQ L +G +  ID+EL +        ++G+ +G YV +  + 
Sbjct: 85  ILNGFPVASGGQLPSGSALQMLMFGTMTVIDDELTE-GHELGSGLLGKAQGYYVASAIDG 144

Query: 190 GEPMIAMRVYLGNGEIKDGLKMFGVYKSNHVKESHITIIGGMGKYLGANGYATLKKSFRT 245
               +A      +G  +D +  FGV ++  V ESHI ++GG GKY+ A G+A L K+F  
Sbjct: 145 TSQTMAFTAMFESGGYEDSISFFGVLRT-AVSESHIGVMGGTGKYVNARGFAIL-KTFTG 204

BLAST of CmoCh04G012870 vs. TAIR 10
Match: AT2G39430.1 (Disease resistance-responsive (dirigent-like protein) family protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.4e-08
Identity = 61/238 (25.63%), Postives = 100/238 (42.02%), Query Frame = 0

Query: 43  LSFLMRDVFDSTSRSSTSKFPANTKTTTNQLPFSKPLGFFLP-RGGIPIPEVYTTTTTTS 102
           L F M DV   +  S+        +T  N +PFSK      P   G+P+       +  +
Sbjct: 87  LEFFMHDVLGGSHPSARVVTGIVAQTEVNGIPFSKASNSIFPVDNGVPLVNSNNINSVIN 146

Query: 103 PTT-------------------------GISAQTPDLSTIGISFPVRAILQELEYGIVRE 162
           P T                          +SA +    T G + P  A LQ L +G +  
Sbjct: 147 PNTAPLLTGLGGAQTSTVIQNTNGNSNDALSANSLPFVTAG-NLPPGAALQHLMFGTITV 206

Query: 163 IDEELFKTSSRKPHVVVGRIKGLYVENNGNSGEPMIAMRVYLGNGE------IKDGLKMF 222
           +D+EL + S      V+GR +G Y+ ++ +     +++ V L +GE      + D +  F
Sbjct: 207 VDDELTE-SHELGSAVIGRAQGFYLASSLDGTSQTLSLTVLL-HGEHDQHDTLDDAISFF 266

Query: 223 GVYK-SNHVKESHITIIGGMGKYLGANGYA---TLKKSFRTKLGANYNKFMELNVYLS 245
           GV++ ++H   S I +IGG GK+  A GYA   TL       +    +  +  +VYL+
Sbjct: 267 GVHRTASHA--SQIAVIGGTGKFEHAKGYAIVETLHNQDNQHITDGQDTILHFSVYLT 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0H88.3e-0926.58Dirigent protein 18 OS=Arabidopsis thaliana OX=3702 GN=DIR18 PE=2 SV=1[more]
Q7Y2251.4e-0826.58Dirigent protein 16 OS=Arabidopsis thaliana OX=3702 GN=DIR16 PE=2 SV=1[more]
Q9SIA81.2e-0728.27Dirigent protein 10 OS=Arabidopsis thaliana OX=3702 GN=DIR10 PE=2 SV=1[more]
O806302.0e-0725.63Dirigent protein 9 OS=Arabidopsis thaliana OX=3702 GN=DIR9 PE=2 SV=1[more]
Q9M3C83.8e-0624.15Dirigent protein 24 OS=Arabidopsis thaliana OX=3702 GN=DIR24 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GYQ59.5e-133100.00Dirigent protein OS=Cucurbita moschata OX=3662 GN=LOC111458697 PE=3 SV=1[more]
A0A6J1JP911.4e-11589.96Dirigent protein OS=Cucurbita maxima OX=3661 GN=LOC111487107 PE=3 SV=1[more]
A0A6J1CCF75.8e-8267.87Dirigent protein OS=Momordica charantia OX=3673 GN=LOC111010220 PE=3 SV=1[more]
A0A5A7V9597.6e-7462.15Dirigent protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G001... [more]
A0A0A0KNT37.6e-7461.66Dirigent protein OS=Cucumis sativus OX=3659 GN=Csa_5G179740 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022957256.12.0e-132100.00dirigent protein 9-like [Cucurbita moschata][more]
XP_022990120.12.8e-11589.96dirigent protein 10-like [Cucurbita maxima][more]
KAG6601020.12.4e-11495.18Dirigent protein 24, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7031632.11.3e-8895.05Dirigent protein 24, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_038891666.13.7e-8366.40dirigent protein 10-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G13580.15.9e-1026.58Disease resistance-responsive (dirigent-like protein) family protein [more]
AT3G24020.11.0e-0926.58Disease resistance-responsive (dirigent-like protein) family protein [more]
AT2G28670.18.5e-0928.27Disease resistance-responsive (dirigent-like protein) family protein [more]
AT2G28670.28.5e-0928.99Disease resistance-responsive (dirigent-like protein) family protein [more]
AT2G39430.11.4e-0825.63Disease resistance-responsive (dirigent-like protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004265Dirigent proteinPFAMPF03018Dirigentcoord: 127..243
e-value: 5.0E-15
score: 55.6
NoneNo IPR availablePANTHERPTHR46215:SF17DIRIGENT PROTEINcoord: 1..243
NoneNo IPR availablePANTHERPTHR46215DIRIGENT PROTEIN 24-RELATEDcoord: 1..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G012870.1CmoCh04G012870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009435 NAD biosynthetic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016020 membrane
molecular_function GO:0004359 glutaminase activity
molecular_function GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity
molecular_function GO:0000166 nucleotide binding