Homology
BLAST of CmoCh04G012260 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 817.4 bits (2110), Expect = 1.3e-235
Identity = 422/720 (58.61%), Postives = 525/720 (72.92%), Query Frame = 0
Query: 18 LISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSAESIVYSYKRSFNG 77
L S S+ DG+ IYIVYMG K +D HLHHRAMLE+VVGS F+ ES++++YKRSFNG
Sbjct: 20 LASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNG 79
Query: 78 FAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPRDVPRLPLQESNIIVGVL 137
FAVKLTEEEA+++A+ EGVVSVF ++ +L TTRSWDF+ FP VPR ESNI+VGVL
Sbjct: 80 FAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVL 139
Query: 138 DTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYRAEGPPPPTQAWGPI 197
DTGIWP+S SFDD+G PPPPKWKG CE+S NFRCNRKIIGAR+Y P P GP
Sbjct: 140 DTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPR 199
Query: 198 DTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVCWERGCSDANILAAF 257
DT+GHGTH AST AG LV A+LYG GLG ARGGVP ARIA YKVCW GCSD +ILAA+
Sbjct: 200 DTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAY 259
Query: 258 DDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSAGNDGPNWYTTGSMA 317
DDAIADGVDI+S+S+GG R +F + IAIG+F A+ GIL SNSAGN GPN++TT S++
Sbjct: 260 DDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLS 319
Query: 318 PWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFDSS 377
PW+LSVAA+T+DR F VQ+GNG +QGV+IN FD + YPLV D+PN GFD S
Sbjct: 320 PWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPNT--GFDKS 379
Query: 378 ISRFCDVNSVDRNLVRGKIVLCDSS---NVSFPSLNGAVGVVMESTASWHLIGTFPLPAS 437
SRFC SV+ NL++GKIV+C++S + F SL+GA GV+M S ++PLP+S
Sbjct: 380 TSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTR-DYADSYPLPSS 439
Query: 438 HFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPNTISPNILKPDLSA 497
+P YI S +P ATIF+S + + +APV +FSSRGPN + +++KPD+S
Sbjct: 440 VLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISG 499
Query: 498 PGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVKTFSPWWSPAAIKSA 557
PGV ILAAWP P+ G ++ +N SGTSM+CPH T IA YVKT++P WSPAAIKSA
Sbjct: 500 PGVEILAAWPSVAPVG-GIRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSA 559
Query: 558 LMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPNITRAQLQ 617
LMTTASPM+ARF+ AEFAYGSG +NPLKAV PGLVY+A+ESDYV++LCG T+A ++
Sbjct: 560 LMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQA-VR 619
Query: 618 QITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNVEATVSTYRATVYSP 677
+ITG+ CT NTGR DLNYPSF LS+ Q+ +F RTLT+V STYRA + +P
Sbjct: 620 RITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRAMISAP 679
Query: 678 PGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSNGVREVRSPIVL 735
GL + V+P L+F+G+GD KSFTL V+G + VSASLVWS+GV VRSPI +
Sbjct: 680 QGLTI--SVNPNVLSFNGLGDRKSFTLTVRGSIKG---FVVSASLVWSDGVHYVRSPITI 727
BLAST of CmoCh04G012260 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 596.3 bits (1536), Expect = 4.8e-169
Identity = 332/734 (45.23%), Postives = 450/734 (61.31%), Query Frame = 0
Query: 11 LNLVC-TLLISASASNGDGRK---IYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSAES 70
L L+C + + S D R+ +YIVYMG+ P+ P HH ++L+++VG+ ++
Sbjct: 8 LYLICLAFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHL 67
Query: 71 IVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPRDVPRLP 130
+V SYKRSFNGFA L++ E+Q++ + VVSVFPSK+++L TTRSWDF+ F R
Sbjct: 68 LVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRES 127
Query: 131 LQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYRAEG 190
++ES++IVGV+D+GIWP+S SFDD+G GPPP KWKG C+ F CN K+IGAR Y
Sbjct: 128 VKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN--- 187
Query: 191 PPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVCWER 250
A D GHGTH AST AG+ V AS YG G ARGGVPSARIA YKVC+ R
Sbjct: 188 ----KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR 247
Query: 251 GCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSAGND 310
C+D +ILAAFDDAIADGVD++SISI V +AIG+F AM GI+ + SAGN+
Sbjct: 248 -CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNN 307
Query: 311 GPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYAGD 370
GP+ + +++PWM++VAA+ DR F V LGNG G+++N F+L G ++P+VY
Sbjct: 308 GPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYG-- 367
Query: 371 VPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS-SNVSFPSLNGAVGVVMESTASWHL 430
N+ + + +C VD LV+GKIVLCD L GA+GV++++T
Sbjct: 368 -QNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDS 427
Query: 431 IGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPNTISP 490
P PAS I+ YI S P A I R+ + D AP +FSSRGP+ +
Sbjct: 428 AFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQ 487
Query: 491 NILKPDLSAPGVNILAAW-PQNVPISHGHP---LSSPYNFASGTSMACPHATAIAAYVKT 550
N+LKPD+SAPG+ ILAA+ P P S +P S Y+ SGTSMACPH +AAYVK+
Sbjct: 488 NLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKS 547
Query: 551 FSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVEY 610
F P WSP+AIKSA+MTTA+PM+ + + EFAYGSGQINP KA +PGLVY DY++
Sbjct: 548 FHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKM 607
Query: 611 LCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNVE 670
LC L +G+++ C++ + DLNYP+ + V F RT+TNV
Sbjct: 608 LCA-EGFDSTTLTTTSGQNVTCSERTEVK--DLNYPTMTTFVSSLDPFNVTFKRTVTNVG 667
Query: 671 ATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVW 730
STY+A+V P LQ ++P L F + + KSF + + G D + VS+S+VW
Sbjct: 668 FPNSTYKASVV-PLQPELQISIEPEILRFGFLEEKKSFVVTISG-KELKDGSFVSSSVVW 725
Query: 731 SNGVREVRSPIVLF 736
S+G VRSPIV +
Sbjct: 728 SDGSHSVRSPIVAY 725
BLAST of CmoCh04G012260 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 590.5 bits (1521), Expect = 2.6e-167
Identity = 326/742 (43.94%), Postives = 453/742 (61.05%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGS-KPKDSSQPHLHHRAMLEEVVG 60
M LA L+ + L +S+ ++ D +++YIVYMGS + P H +L+EV G
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60
Query: 61 SAFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP 120
+ +V SYKRSFNGFA +LTE E +R+A GVVSVFP+K QLQTT SWDF+
Sbjct: 61 ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120
Query: 121 RDV--PRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKII 180
+ R P ES+ I+GV+D+GI P+S SF D+G GPPP KWKG+C NF CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180
Query: 181 GARAYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARI 240
GAR Y +EG D GHGTH AST AG+ V +AS +G G G RGGVP++R+
Sbjct: 181 GARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240
Query: 241 AVYKVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGI 300
A YKVC GCS +L+AFDDAIADGVD+++ISIG + F + IAIGAF AM G+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300
Query: 301 LVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
L NSAGN GP + +APW+L+VAA+T +R F V LGNG G ++N +++ GK
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360
Query: 361 QYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS-SNVSFPSLNGAVGVV 420
YPLVY + D+ + C+++ VD++ V+GKI++C + GAVG++
Sbjct: 361 DYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLI 420
Query: 421 MESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFS 480
T + PLPA+ + Y+ S +P A + ++ + + +PV A+FS
Sbjct: 421 YR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 480
Query: 481 SRGPNTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIA 540
SRGPNTI+ +ILKPD++APGV ILAA+ S Y+ SGTSM+CPH +A
Sbjct: 481 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVA 540
Query: 541 AYVKTFSPWWSPAAIKSALMTTASPMDARFS--QGAEFAYGSGQINPLKAVNPGLVYNAS 600
AYVKTF+P WSP+ I+SA+MTTA P++A + EFAYGSG ++P+ A NPGLVY
Sbjct: 541 AYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELD 600
Query: 601 ESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSM-GRGQSLRVFF 660
+SD++ +LCG+ N T L+ I+GE + C+++ +LNYPS + + G G + V F
Sbjct: 601 KSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 660
Query: 661 PRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPA 720
RTLTNV STY + V + G L K+ P L+F + + +SFT+ V G +
Sbjct: 661 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG-SNLDSEV 720
Query: 721 TVSASLVWSNGVREVRSPIVLF 736
SA+L+WS+G VRSPIV++
Sbjct: 721 PSSANLIWSDGTHNVRSPIVVY 728
BLAST of CmoCh04G012260 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 583.2 bits (1502), Expect = 4.2e-165
Identity = 334/747 (44.71%), Postives = 456/747 (61.04%), Query Frame = 0
Query: 5 ALRVVFLNLVCTLLISASASNG---DGRKIYIVYMGSKP-KDSSQPHLHHRAMLEEVVGS 64
A+ L+ + LL+ + AS G +++YIVYMG+ P + P HH ++L++V G
Sbjct: 4 AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63
Query: 65 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP- 124
+ + +V +YKRSFNGFA +LTE E + +A+ + VVSVFPSKN LQTT SW+F+
Sbjct: 64 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123
Query: 125 -RDVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIG 184
+ R PL ES+ I+GV+D+GI+P+S SF +G GPPP KWKG+C+ NF CN K+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183
Query: 185 ARAY--RAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSAR 244
AR Y + EG P + D +GHG+H AS AG+ V + S YG G G RGGVP+AR
Sbjct: 184 ARYYTPKLEGFPESAR-----DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAAR 243
Query: 245 IAVYKVCWERG---CSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAM 304
IAVYKVC + G C+ ILAAFDDAIAD VDI+++S+G V F E+ +AIGAF AM
Sbjct: 244 IAVYKVC-DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAM 303
Query: 305 RNGILVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFD 364
GIL N AGN+GP T SMAPW+ +VAA+ ++R F V LGNG G ++N FD
Sbjct: 304 AKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFD 363
Query: 365 LGGKQYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSS-NVSFPSLNGA 424
L GK+YPLVY + D+S + FC +D V+GKIVLCD+ N GA
Sbjct: 364 LNGKKYPLVYG---KSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGA 423
Query: 425 VGVVMESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVA 484
V ++ + F P S + N + Y+ S P A + +S + + APV
Sbjct: 424 VASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVV 483
Query: 485 AAFSSRGPNTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHA 544
A++SSRGPN + +ILKPD++APG ILAA+ VP S Y SGTSM+CPH
Sbjct: 484 ASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHV 543
Query: 545 TAIAAYVKTFSPWWSPAAIKSALMTTASPMDARFSQG---AEFAYGSGQINPLKAVNPGL 604
+AAY+KTF P WSP+ I+SA+MTTA PM+A S AEFAYG+G ++P+ A++PGL
Sbjct: 544 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 603
Query: 605 VYNASESDYVEYLCGIPNITRAQLQQITGEDIICTDSNT-GRDLDLNYPSFALSMGRGQS 664
VY A++SD++ +LCG N T +L+ I+G+ CT T +LNYPS + + +
Sbjct: 604 VYEANKSDHITFLCGF-NYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 663
Query: 665 LRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLS 724
+V F RT+TNV +TY+A V G L+ KV P L+ + + KSFT+ V G
Sbjct: 664 FKVTFRRTVTNVGRPNATYKAKVV---GSKLKVKVVPAVLSLKSLYEKKSFTVTVSG-AG 723
Query: 725 ATDPATVSASLVWSNGVREVRSPIVLF 736
VSA L+WS+GV VRSPIV++
Sbjct: 724 PKAENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmoCh04G012260 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 581.6 bits (1498), Expect = 1.2e-164
Identity = 326/730 (44.66%), Postives = 444/730 (60.82%), Query Frame = 0
Query: 13 LVCTLLISASASNGDGRKIYIVYMGSKPKDSSQ-PHLHHRAMLEEVVGSAFSAESIVYSY 72
L+ LL S SA + ++YIVYMGS + P H ++L++V G + +V SY
Sbjct: 14 LLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSY 73
Query: 73 KRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP--RDVPRLPLQE 132
KRSFNGFA +LTE E +A EGVVSVFP+K QL TT SWDF+ ++ R E
Sbjct: 74 KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 133
Query: 133 SNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYRAEGPPP 192
S+ I+GV+DTGIWP+S SF D+G GPPP KWKG+C NF CN K+IGAR Y +EG
Sbjct: 134 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR- 193
Query: 193 PTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVCWERGCS 252
DTSGHGTH AST AG+ V + S +G G G RGGVP++RIA YKVC + GCS
Sbjct: 194 --------DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 253
Query: 253 DANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSAGNDGPN 312
+L++FDDAIADGVD+++ISIG + F ++ IAIGAF AM GIL +SAGN GP
Sbjct: 254 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 313
Query: 313 WYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPN 372
T +APW+ +VAA+T +R F V LGNG G ++N FD+ GK+YPLVY +
Sbjct: 314 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 373
Query: 373 IGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS-SNVSFPSLNGAVGVVMESTASWHLIGT 432
D+ + C ++++ V+GKI++C S GA+ ++ +S + T
Sbjct: 374 --SACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRP-DVAFT 433
Query: 433 FPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPNTISPNIL 492
LPAS + YI S +P A + ++ + + +PV A+FSSRGPNTI+ +IL
Sbjct: 434 HHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDIL 493
Query: 493 KPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVKTFSPWWSP 552
KPD++APGV ILAA+ N S Y+ SGTSMACPH +AAYVKTF P WSP
Sbjct: 494 KPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSP 553
Query: 553 AAIKSALMTTASPMDA--RFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIP 612
+ I+SA+MTTA P+ A R EFAYG+G ++P+ A+NPGLVY ++D++ +LCG+
Sbjct: 554 SMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGM- 613
Query: 613 NITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSM-GRGQSLRVFFPRTLTNVEATVS 672
N T L+ I+G+ + C+ N +LNYPS + + G + V F RTLTNV S
Sbjct: 614 NYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNS 673
Query: 673 TYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSNGV 732
TY++ V + G L KV P L F + + +SF++ V G SA+L+WS+G
Sbjct: 674 TYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG-SDVDSEVPSSANLIWSDGT 729
Query: 733 REVRSPIVLF 736
VRSPIV++
Sbjct: 734 HNVRSPIVVY 729
BLAST of CmoCh04G012260 vs. ExPASy TrEMBL
Match:
A0A6J1GX71 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458261 PE=3 SV=1)
HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 725/735 (98.64%), Postives = 730/735 (99.32%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MCCLALRVVFLNL+CT LISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS
Sbjct: 1 MCCLALRVVFLNLLCTSLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNY+LQTTRSWDFI FPR
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYKLQTTRSWDFIGFPR 120
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGVLDTGIWPDSLSF DQGLG PPPKWKGICE+SANFRCNRKIIGAR
Sbjct: 121 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGICEASANFRCNRKIIGAR 180
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS
Sbjct: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST
Sbjct: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP
Sbjct: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK
Sbjct: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
Query: 721 WSNGVREVRSPIVLF 736
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 735
BLAST of CmoCh04G012260 vs. ExPASy TrEMBL
Match:
A0A6J1IMT3 (cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1)
HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 665/735 (90.48%), Postives = 690/735 (93.88%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MC LALR+VFLNL+ TLLI+ SASNGDGRKIYIVYMGSKPKDSSQ HLHHRAMLEEVVGS
Sbjct: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKN +LQTTRSWDFI F R
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGV DTGIWPDSLSF DQGLGPPPPKWKG CE+SANFRCNRKIIGAR
Sbjct: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRA GP PPT+AWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCW R C DA++LAAFDDAIADGVDILS+SIGG VRPFF +GIAIGA+QAMRNGIL S
Sbjct: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMRNGILTS 300
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGS+APWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNIGGGF+S+ISRFC VNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME T
Sbjct: 361 LVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGT 420
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
+W LI TFPLPASHFNPPSANPIRLYITSNPAPTATIFRSV VN TNAPVAA FSSRGP
Sbjct: 421 TTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGP 480
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
N ISPNILKPDLSAPGVNILAAWPQNVPIS G PLSSPYNFASGTSMACPH TAIAAYVK
Sbjct: 481 NAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVK 540
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TF+PWWSPAAIKSALMTTA PMD + ++ AEF+YGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIP IT AQL+ ITGEDIICTDSNTGRDLDLNYPSFAL MGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLV 720
Query: 721 WSNGVREVRSPIVLF 736
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 734
BLAST of CmoCh04G012260 vs. ExPASy TrEMBL
Match:
A0A6J1IP33 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1)
HSP 1 Score: 1285.4 bits (3325), Expect = 0.0e+00
Identity = 636/700 (90.86%), Postives = 658/700 (94.00%), Query Frame = 0
Query: 36 MGSKPKDSSQPHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEG 95
MGSKPKDSSQ HLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEG
Sbjct: 1 MGSKPKDSSQAHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEG 60
Query: 96 VVSVFPSKNYQLQTTRSWDFINFPRDVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGP 155
VVSVFPSKN +LQTTRSWDFI F RDVPR+P QES+IIVGV DTGIWPDSLSF DQGLGP
Sbjct: 61 VVSVFPSKNNKLQTTRSWDFIGFTRDVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGP 120
Query: 156 PPPKWKGICESSANFRCNRKIIGARAYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLV 215
PPPKWKG CE+SANFRCNRKIIGARAYRA GP PPT+AWGPIDT+GHGTHVASTVAG LV
Sbjct: 121 PPPKWKGTCEASANFRCNRKIIGARAYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLV 180
Query: 216 DNASLYGFGLGLARGGVPSARIAVYKVCWERGCSDANILAAFDDAIADGVDILSISIGGR 275
DNASLYGFGLGLARGGVPSARIAVYKVCW R C DA++LAAFDDAIADGVDILS+SIGG
Sbjct: 181 DNASLYGFGLGLARGGVPSARIAVYKVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG- 240
Query: 276 RVRPFFENGIAIGAFQAMRNGILVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAH 335
VRPFF +GIAIGA+QAMRNGIL SNSAGNDGPNWYTTGS+APWMLSVAANTIDRNFAAH
Sbjct: 241 LVRPFFHDGIAIGAYQAMRNGILTSNSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAH 300
Query: 336 VQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGK 395
VQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGF+S+ISRFC VNSVDRNLVRGK
Sbjct: 301 VQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGK 360
Query: 396 IVLCDSSNVSFPSLNGAVGVVMESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPT 455
IVLCDSS SFPSLNGAVGVVME T +W LI TFPLPASHFNPPSANPIRLYITSNPAPT
Sbjct: 361 IVLCDSSGASFPSLNGAVGVVMEGTTTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPT 420
Query: 456 ATIFRSVNVNDTNAPVAAAFSSRGPNTISPNILKPDLSAPGVNILAAWPQNVPISHGHPL 515
ATIFRSV VN TNAPVAA FSSRGPN ISPNILKPDLSAPGVNILAAWPQNVPIS G PL
Sbjct: 421 ATIFRSVEVNHTNAPVAADFSSRGPNAISPNILKPDLSAPGVNILAAWPQNVPISPGDPL 480
Query: 516 SSPYNFASGTSMACPHATAIAAYVKTFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYG 575
SSPYNFASGTSMACPH TAIAAYVKTF+PWWSPAAIKSALMTTA PMD + ++ AEF+YG
Sbjct: 481 SSPYNFASGTSMACPHVTAIAAYVKTFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYG 540
Query: 576 SGQINPLKAVNPGLVYNASESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLN 635
SGQINPLKAVNPGLVYNASESDYVEYLCGIP IT AQL+ ITGEDIICTDSNTGRDLDLN
Sbjct: 541 SGQINPLKAVNPGLVYNASESDYVEYLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLN 600
Query: 636 YPSFALSMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGD 695
YPSFAL MGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGD
Sbjct: 601 YPSFALFMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGD 660
Query: 696 TKSFTLGVQGWLSATDPATVSASLVWSNGVREVRSPIVLF 736
TKSFTLGVQGWLSATDP TVSASLVWSNGVREVRSPIVLF
Sbjct: 661 TKSFTLGVQGWLSATDPTTVSASLVWSNGVREVRSPIVLF 699
BLAST of CmoCh04G012260 vs. ExPASy TrEMBL
Match:
K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)
HSP 1 Score: 874.4 bits (2258), Expect = 3.4e-250
Identity = 452/738 (61.25%), Postives = 558/738 (75.61%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
M L +++FL+L C+LL+S+S SN DGRKIYIVYMGSK +D++ HL+HRAMLEEVVGS
Sbjct: 6 MSSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGS 65
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
F+ ES++Y+YKRSFNGFAVKLTEEEA ++AAKEGVVSVFPS+ L TTRSWDF+ +
Sbjct: 66 TFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQ 125
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
+VPR+ ESNI+VGV D+GIWP++ SF+D G GP P W+G C++S NFRCNRKIIGAR
Sbjct: 126 NVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGAR 185
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYR+ PP P DT GHGTH ASTVAG LV ASLYG G+G ARGGVP ARIAVY
Sbjct: 186 AYRS-STLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVY 245
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
K+CW GCSDA+ILAAFDDAIADGVDI+S+S+GG+ +P+ N IAIG+F AM+ GIL S
Sbjct: 246 KICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTS 305
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGN+GP +T S++PW+ +VAA++ DR F V LGNGN YQGV+IN FD+ QYP
Sbjct: 306 NSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDM-RNQYP 365
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSS--NVSFPSLNGAVGVVME 420
L+YAG+ P+I GF+SS SR+C +SVD NLVRGKI+LCDS+ F S GA GV+M+
Sbjct: 366 LIYAGNAPSI--GFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQ 425
Query: 421 STASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSR 480
S H ++PLPAS +P N I+ Y++S APTATIF+S V DT+APV +FSSR
Sbjct: 426 SNTRDH-ASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSR 485
Query: 481 GPNTISPNILKPDLSAPGVNILAAWPQNVPISH-GHPLSSPYNFASGTSMACPHATAIAA 540
GPN ++ +ILKPD +APGV ILAAWP PIS S+ YN SGTSM+CPH TAIA
Sbjct: 486 GPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAV 545
Query: 541 YVKTFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESD 600
++KTF P WSPAAIKSALMTTASPM+ARF+ AEFAYGSG +NPLKAV+PGLVY+ASESD
Sbjct: 546 HIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESD 605
Query: 601 YVEYLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTL 660
YV++LCG T A ++ TG++ CT N GR DLNYPSFALS+ R Q+ F RTL
Sbjct: 606 YVKFLCG-EGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTL 665
Query: 661 TNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSA 720
TNV + STYRA++ +P GL++ V+P L+F+GIGD KSFTL V+G +S A VSA
Sbjct: 666 TNVVSGASTYRASISAPQGLSI--SVNPSVLSFNGIGDQKSFTLTVRGTVS---QAIVSA 725
Query: 721 SLVWSNGVREVRSPIVLF 736
SLVWS+G VRSPI ++
Sbjct: 726 SLVWSDGSHNVRSPITVY 732
BLAST of CmoCh04G012260 vs. ExPASy TrEMBL
Match:
A0A6J1CBL9 (LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC111010158 PE=3 SV=1)
HSP 1 Score: 869.8 bits (2246), Expect = 8.3e-249
Identity = 446/742 (60.11%), Postives = 546/742 (73.58%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
M L + +F +L LL+S S + DGRK YIVYMGSKP+D++ LHHRAMLEEV+GS
Sbjct: 7 MSSLIFKFIFFSLFYRLLVSGSHIDNDGRKTYIVYMGSKPEDTTSTPLHHRAMLEEVIGS 66
Query: 61 AF---SAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFIN 120
F + ES++YSYKRSFNGFAV+LTEEEA ++AAKEGVVSVFP+ L TTRSWDF+
Sbjct: 67 TFFLAAPESLLYSYKRSFNGFAVRLTEEEALKVAAKEGVVSVFPNGKKHLHTTRSWDFMG 126
Query: 121 FPRDVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKII 180
F + VPR ES+++VGVLDTGIWP+S SF D+ GPPP KW G C+++ +FRCN+KII
Sbjct: 127 FSQSVPRAHQVESDVVVGVLDTGIWPESPSFIDEAFGPPPAKWMGACQAADDFRCNKKII 186
Query: 181 GARAYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARI 240
GAR YR+E PP+ P D++GHGTH ASTVAG LV+ ASLYG G+G ARGGVPSARI
Sbjct: 187 GARVYRSES-LPPSDIRSPRDSNGHGTHTASTVAGGLVEQASLYGLGIGTARGGVPSARI 246
Query: 241 AVYKVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGI 300
AVYK+CW GCSDA+ILAAFDDAIADGVDI+S+S+GG +F + IAIGAF AM++GI
Sbjct: 247 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGSEPNSYFSDPIAIGAFHAMKHGI 306
Query: 301 LVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
L SNSAGN+GPN++T +++PW LSVAA+T DR VQLGNGN+YQGVAIN FDL GK
Sbjct: 307 LTSNSAGNEGPNYFTASNISPWSLSVAASTTDRKILTQVQLGNGNIYQGVAINTFDLLGK 366
Query: 361 QYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS--SNVSFPSLNGAVGV 420
QYPL+YAGD PNI GGF+ SISR C NSVD+NLVRGKI++CDS S N VG+
Sbjct: 367 QYPLIYAGDAPNIAGGFNGSISRLCVENSVDKNLVRGKILVCDSLLGASKLASFNATVGI 426
Query: 421 VMESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAF 480
VM+ ++P PAS+ + + I+LYI+S PTATI +S+ V D +APV +F
Sbjct: 427 VMQDLRLKDYASSYPFPASYLRTAATDDIQLYISSTTDPTATILKSIAVQDASAPVVVSF 486
Query: 481 SSRGPNTISPNILKPDLSAPGVNILAAWPQNVPI--SHGHPLSSPYNFASGTSMACPHAT 540
SSRGP I+ +ILK DLSAPGV ILAAW P+ G S YN SGTSM+CPHAT
Sbjct: 487 SSRGPKFITRDILKSDLSAPGVEILAAWSPLAPVLGVKGDSRSELYNIISGTSMSCPHAT 546
Query: 541 AIAAYVKTFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNA 600
AIAAYVKTF P WSPAA+KSALMTTA M+A+ + AEFAYGSG +NPLKAVNPGLVY+A
Sbjct: 547 AIAAYVKTFHPTWSPAALKSALMTTAFTMNAKLNPQAEFAYGSGHVNPLKAVNPGLVYDA 606
Query: 601 SESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFF 660
+E DYV++LCG + A +++ITG+ CT N GR DLNYPSFALS + + FF
Sbjct: 607 NEGDYVKFLCG-QGYSTAMVRRITGDSSACTPGNIGRVWDLNYPSFALSTAPSRPINQFF 666
Query: 661 PRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPA 720
RTLTNVE+ STY A V+ PP L V+P L+F+G+GDTKSF L VQG +S A
Sbjct: 667 RRTLTNVESGASTYSAVVFGPPD-GLHITVNPSVLSFNGVGDTKSFNLTVQGTVS---QA 726
Query: 721 TVSASLVWSNGVREVRSPIVLF 736
VSASL WS+GV +VRSPI ++
Sbjct: 727 IVSASLEWSDGVHQVRSPITIY 742
BLAST of CmoCh04G012260 vs. NCBI nr
Match:
XP_022956553.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1466.1 bits (3794), Expect = 0.0e+00
Identity = 725/735 (98.64%), Postives = 730/735 (99.32%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MCCLALRVVFLNL+CT LISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS
Sbjct: 1 MCCLALRVVFLNLLCTSLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNY+LQTTRSWDFI FPR
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYKLQTTRSWDFIGFPR 120
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGVLDTGIWPDSLSF DQGLG PPPKWKGICE+SANFRCNRKIIGAR
Sbjct: 121 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGICEASANFRCNRKIIGAR 180
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS
Sbjct: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST
Sbjct: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP
Sbjct: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK
Sbjct: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
Query: 721 WSNGVREVRSPIVLF 736
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 735
BLAST of CmoCh04G012260 vs. NCBI nr
Match:
XP_023548098.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 673/735 (91.56%), Postives = 695/735 (94.56%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MCCLALRVVFLNL+ TLLI+ SASNGDGRKIYIVYMGSKP DSSQPHLHHRAMLEEVVGS
Sbjct: 14 MCCLALRVVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPTDSSQPHLHHRAMLEEVVGS 73
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLT+EEAQRMA KEGVVSVFPSKNY+LQTTRSWDFI FP
Sbjct: 74 AFSAESIVYSYKRSFNGFAVKLTDEEAQRMADKEGVVSVFPSKNYKLQTTRSWDFIGFPP 133
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGVLDTGIWPDSLSF DQGLG PPPKWKG CE+SANFRCNRKIIGAR
Sbjct: 134 DVPRVPQQESDIIVGVLDTGIWPDSLSFHDQGLGAPPPKWKGTCEASANFRCNRKIIGAR 193
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAEGP PPTQAWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 194 AYRAEGPLPPTQAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 253
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCWERGCSDANILAAFDDAIADGVDILS+SIG VRPFFE+GIAIGA+QAMRNGIL S
Sbjct: 254 KVCWERGCSDANILAAFDDAIADGVDILSLSIGD-LVRPFFEDGIAIGAYQAMRNGILTS 313
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
N AGN G NWYTTGSMAPWMLSVAANTIDRNF +HVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 314 NGAGNGGTNWYTTGSMAPWMLSVAANTIDRNFVSHVQLGNGNVYQGVAINLFDLGGKQYP 373
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNI GGF SS SRFC VNSVDRNLVRGKIVLCDSS VSFPSLNGAVGVVMEST
Sbjct: 374 LVYAGDVPNIAGGFSSSTSRFCGVNSVDRNLVRGKIVLCDSSAVSFPSLNGAVGVVMEST 433
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
+WHLI TFPLPASHFNPPS NPIRLYITSNPAPTATIFRSV+VN TNAPVAA FSSRGP
Sbjct: 434 TTWHLIRTFPLPASHFNPPSGNPIRLYITSNPAPTATIFRSVDVNHTNAPVAADFSSRGP 493
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
N ISPNILKPDLSAPGVNILAAWPQNVPIS G+PL SPYNFASGTSMACPHATAIAAYVK
Sbjct: 494 NAISPNILKPDLSAPGVNILAAWPQNVPISPGNPLLSPYNFASGTSMACPHATAIAAYVK 553
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TFSPWWSPAAIKSALMTTASPMDA+ + AEF++GSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 554 TFSPWWSPAAIKSALMTTASPMDAKVNTQAEFSHGSGQINPLKAVNPGLVYNASESDYVE 613
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIP ITRAQL+ ITGEDIICTDSNTGRD+DLNYPSFAL MGRGQSLRVFFPRTLTNV
Sbjct: 614 YLCGIPGITRAQLRLITGEDIICTDSNTGRDVDLNYPSFALFMGRGQSLRVFFPRTLTNV 673
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLV
Sbjct: 674 EATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLV 733
Query: 721 WSNGVREVRSPIVLF 736
WSNGVREVRSPIVLF
Sbjct: 734 WSNGVREVRSPIVLF 747
BLAST of CmoCh04G012260 vs. NCBI nr
Match:
XP_023547983.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1348.6 bits (3489), Expect = 0.0e+00
Identity = 671/732 (91.67%), Postives = 693/732 (94.67%), Query Frame = 0
Query: 5 ALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSA 64
ALRVVFLNL+CTL+ISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSA
Sbjct: 121 ALRVVFLNLLCTLIISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGSAFSA 180
Query: 65 ESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPRDVPR 124
ESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKN +LQTTRSWDFI FP DVPR
Sbjct: 181 ESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFPPDVPR 240
Query: 125 LPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYRA 184
+P QESNIIVGVLD+GIWPDSLSFDDQGLGPPPPKWKG CE+SANFRCNRKIIGARAYRA
Sbjct: 241 VPQQESNIIVGVLDSGIWPDSLSFDDQGLGPPPPKWKGTCEASANFRCNRKIIGARAYRA 300
Query: 185 EGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVCW 244
EGP PPT+AWGPIDTSGHGTHVASTVAG LVDNA LYGFG+GLARGGVPSARIAVYKVCW
Sbjct: 301 EGPLPPTEAWGPIDTSGHGTHVASTVAGRLVDNAGLYGFGVGLARGGVPSARIAVYKVCW 360
Query: 245 ERG-CSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSA 304
E G CSDANILAA DDAIADGVDILS+SI VRPFFE+ IAIGA+QAMRNGIL SNSA
Sbjct: 361 ENGWCSDANILAAIDDAIADGVDILSLSITS-LVRPFFEDAIAIGAYQAMRNGILTSNSA 420
Query: 305 GNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVY 364
GN GP+W TTGS APWMLSVAANTIDRNF + VQLGNGNVYQGVAINLFDLGGKQYPLVY
Sbjct: 421 GNRGPDWSTTGSTAPWMLSVAANTIDRNFQSQVQLGNGNVYQGVAINLFDLGGKQYPLVY 480
Query: 365 AGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMESTASW 424
AGDVPNI GGF SS SRFC VNSVDRNLVRGKIVLCDSS VSFPSLNGAVGVVMEST +W
Sbjct: 481 AGDVPNIAGGFSSSTSRFCGVNSVDRNLVRGKIVLCDSSAVSFPSLNGAVGVVMESTTTW 540
Query: 425 HLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPNTI 484
HLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAA FSSRGPN I
Sbjct: 541 HLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAADFSSRGPNAI 600
Query: 485 SPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVKTFS 544
SPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSM+CPHATAIA YVKTFS
Sbjct: 601 SPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMSCPHATAIAVYVKTFS 660
Query: 545 PWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVEYLC 604
PWWSPAAIKSALMTTASPMDA+ + AEF++GSGQINPLKAVNPGLVYNASESDYVEYLC
Sbjct: 661 PWWSPAAIKSALMTTASPMDAKVNTQAEFSHGSGQINPLKAVNPGLVYNASESDYVEYLC 720
Query: 605 GIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNVEAT 664
GIP ITRAQL+ ITGEDIICTD NTGRD+DLNYPSFAL MGRGQSLRVFFPRTLTNVEAT
Sbjct: 721 GIPGITRAQLRLITGEDIICTDINTGRDVDLNYPSFALFMGRGQSLRVFFPRTLTNVEAT 780
Query: 665 VSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSN 724
VSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSN
Sbjct: 781 VSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSN 840
Query: 725 GVREVRSPIVLF 736
GVREVRSPIVLF
Sbjct: 841 GVREVRSPIVLF 851
BLAST of CmoCh04G012260 vs. NCBI nr
Match:
XP_022976643.1 (cucumisin-like isoform X2 [Cucurbita maxima])
HSP 1 Score: 1337.4 bits (3460), Expect = 0.0e+00
Identity = 665/735 (90.48%), Postives = 690/735 (93.88%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MC LALR+VFLNL+ TLLI+ SASNGDGRKIYIVYMGSKPKDSSQ HLHHRAMLEEVVGS
Sbjct: 1 MCSLALRLVFLNLLFTLLITTSASNGDGRKIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKN +LQTTRSWDFI F R
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNNKLQTTRSWDFIGFTR 120
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGV DTGIWPDSLSF DQGLGPPPPKWKG CE+SANFRCNRKIIGAR
Sbjct: 121 DVPRIPQQESDIIVGVFDTGIWPDSLSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRA GP PPT+AWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAVGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCW R C DA++LAAFDDAIADGVDILS+SIGG VRPFF +GIAIGA+QAMRNGIL S
Sbjct: 241 KVCWGRTCEDADMLAAFDDAIADGVDILSLSIGG-LVRPFFHDGIAIGAYQAMRNGILTS 300
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
NSAGNDGPNWYTTGS+APWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP
Sbjct: 301 NSAGNDGPNWYTTGSLAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNIGGGF+S+ISRFC VNSVDRNLVRGKIVLCDSS SFPSLNGAVGVVME T
Sbjct: 361 LVYAGDVPNIGGGFNSNISRFCGVNSVDRNLVRGKIVLCDSSGASFPSLNGAVGVVMEGT 420
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
+W LI TFPLPASHFNPPSANPIRLYITSNPAPTATIFRSV VN TNAPVAA FSSRGP
Sbjct: 421 TTWQLIRTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVEVNHTNAPVAADFSSRGP 480
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
N ISPNILKPDLSAPGVNILAAWPQNVPIS G PLSSPYNFASGTSMACPH TAIAAYVK
Sbjct: 481 NAISPNILKPDLSAPGVNILAAWPQNVPISPGDPLSSPYNFASGTSMACPHVTAIAAYVK 540
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TF+PWWSPAAIKSALMTTA PMD + ++ AEF+YGSGQINPLKAVNPGLVYNASESDYVE
Sbjct: 541 TFNPWWSPAAIKSALMTTAFPMDVKVNRQAEFSYGSGQINPLKAVNPGLVYNASESDYVE 600
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIP IT AQL+ ITGEDIICTDSNTGRDLDLNYPSFAL MGRGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPGITPAQLRLITGEDIICTDSNTGRDLDLNYPSFALFMGRGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EATVSTYRATVYSPPGLN+QFKVDPPALTFHGIGDTKSFTLGVQGWLSATDP TVSASLV
Sbjct: 661 EATVSTYRATVYSPPGLNVQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPTTVSASLV 720
Query: 721 WSNGVREVRSPIVLF 736
WSNGVREVRSPIVLF
Sbjct: 721 WSNGVREVRSPIVLF 734
BLAST of CmoCh04G012260 vs. NCBI nr
Match:
KAG6600969.1 (hypothetical protein SDJN03_06202, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1305.4 bits (3377), Expect = 0.0e+00
Identity = 655/735 (89.12%), Postives = 684/735 (93.06%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGSKPKDSSQPHLHHRAMLEEVVGS 60
MC LALRVVFLNL+CTLLISASASNGDGR+IYIVYMGSKPKDSSQ HLHHRAMLEEVVGS
Sbjct: 1 MCRLALRVVFLNLLCTLLISASASNGDGREIYIVYMGSKPKDSSQAHLHHRAMLEEVVGS 60
Query: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPR 120
AFSAESIVYSYKRSFNGFAVKLTEEEAQRMA KEGVVSVFPSKNY+LQTTRSWDFI+FPR
Sbjct: 61 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMADKEGVVSVFPSKNYKLQTTRSWDFISFPR 120
Query: 121 DVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGAR 180
DVPR+P QES+IIVGVLDTGIWPDS SF DQGLGPPPPKWKG CE+SANFRCNRKIIGAR
Sbjct: 121 DVPRVPEQESDIIVGVLDTGIWPDSFSFHDQGLGPPPPKWKGTCEASANFRCNRKIIGAR 180
Query: 181 AYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVY 240
AYRAEGP PPT+AWGPIDT+GHGTHVASTVAG LVDNASLYGFGLGLARGGVPSARIAVY
Sbjct: 181 AYRAEGPLPPTEAWGPIDTNGHGTHVASTVAGRLVDNASLYGFGLGLARGGVPSARIAVY 240
Query: 241 KVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVS 300
KVCWE GCSDAN+LAAFDDAIADGVDILS+SIGG RV P+FE+GIAIGA+QAMRNGIL S
Sbjct: 241 KVCWENGCSDANMLAAFDDAIADGVDILSLSIGG-RVIPYFEDGIAIGAYQAMRNGILTS 300
Query: 301 NSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYP 360
N+AGNDGPN YTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNV+QGVAINLFDL GKQYP
Sbjct: 301 NAAGNDGPNGYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVHQGVAINLFDLEGKQYP 360
Query: 361 LVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLNGAVGVVMEST 420
LVYAGDVPNI GGF SS S RGKIVLCDSS+VSFPSLNGAVGVVMEST
Sbjct: 361 LVYAGDVPNIAGGFSSSTS-------------RGKIVLCDSSDVSFPSLNGAVGVVMEST 420
Query: 421 ASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGP 480
+ H I TFPLPASHFNPPSA+PIRLY+TSNPAPTATIFRSV VN T+APVAA FSSRGP
Sbjct: 421 TTHHWIRTFPLPASHFNPPSASPIRLYMTSNPAPTATIFRSVEVNHTDAPVAADFSSRGP 480
Query: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVK 540
NTISPNILKPDLSAPGVNILAAWPQNVPIS GHP SSPYNF SGTSMACPHATAIAAYVK
Sbjct: 481 NTISPNILKPDLSAPGVNILAAWPQNVPISPGHPFSSPYNFLSGTSMACPHATAIAAYVK 540
Query: 541 TFSPWWSPAAIKSALMTTASPMDARFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVE 600
TF+PWWSPAAIKSALMTTASPMDARFS GAEFAYGSGQINPLKAVNPGLVYNASESDY +
Sbjct: 541 TFNPWWSPAAIKSALMTTASPMDARFSLGAEFAYGSGQINPLKAVNPGLVYNASESDYEK 600
Query: 601 YLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSMGRGQSLRVFFPRTLTNV 660
YLCGIP+IT AQL+QITGE+IICT+SNTGRD+DLNYPSFAL M RGQSLRVFFPRTLTNV
Sbjct: 601 YLCGIPDITPAQLRQITGENIICTESNTGRDVDLNYPSFALFMERGQSLRVFFPRTLTNV 660
Query: 661 EATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLV 720
EA VSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLS TDPA VSASLV
Sbjct: 661 EARVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSTTDPALVSASLV 720
Query: 721 WSNGVREVRSPIVLF 736
WSNG EVRSPI+LF
Sbjct: 721 WSNGFGEVRSPIILF 721
BLAST of CmoCh04G012260 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 590.5 bits (1521), Expect = 1.9e-168
Identity = 326/742 (43.94%), Postives = 453/742 (61.05%), Query Frame = 0
Query: 1 MCCLALRVVFLNLVCTLLISASASNGDGRKIYIVYMGS-KPKDSSQPHLHHRAMLEEVVG 60
M LA L+ + L +S+ ++ D +++YIVYMGS + P H +L+EV G
Sbjct: 1 MATLAASSSLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 60
Query: 61 SAFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP 120
+ +V SYKRSFNGFA +LTE E +R+A GVVSVFP+K QLQTT SWDF+
Sbjct: 61 ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 120
Query: 121 RDV--PRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKII 180
+ R P ES+ I+GV+D+GI P+S SF D+G GPPP KWKG+C NF CN K+I
Sbjct: 121 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 180
Query: 181 GARAYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARI 240
GAR Y +EG D GHGTH AST AG+ V +AS +G G G RGGVP++R+
Sbjct: 181 GARDYTSEGTR---------DMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 240
Query: 241 AVYKVCWERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGI 300
A YKVC GCS +L+AFDDAIADGVD+++ISIG + F + IAIGAF AM G+
Sbjct: 241 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 300
Query: 301 LVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGK 360
L NSAGN GP + +APW+L+VAA+T +R F V LGNG G ++N +++ GK
Sbjct: 301 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 360
Query: 361 QYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS-SNVSFPSLNGAVGVV 420
YPLVY + D+ + C+++ VD++ V+GKI++C + GAVG++
Sbjct: 361 DYPLVYGKSAAS--SACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVGLI 420
Query: 421 MESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFS 480
T + PLPA+ + Y+ S +P A + ++ + + +PV A+FS
Sbjct: 421 YR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 480
Query: 481 SRGPNTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIA 540
SRGPNTI+ +ILKPD++APGV ILAA+ S Y+ SGTSM+CPH +A
Sbjct: 481 SRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVA 540
Query: 541 AYVKTFSPWWSPAAIKSALMTTASPMDARFS--QGAEFAYGSGQINPLKAVNPGLVYNAS 600
AYVKTF+P WSP+ I+SA+MTTA P++A + EFAYGSG ++P+ A NPGLVY
Sbjct: 541 AYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELD 600
Query: 601 ESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSM-GRGQSLRVFF 660
+SD++ +LCG+ N T L+ I+GE + C+++ +LNYPS + + G G + V F
Sbjct: 601 KSDHIAFLCGM-NYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 660
Query: 661 PRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPA 720
RTLTNV STY + V + G L K+ P L+F + + +SFT+ V G +
Sbjct: 661 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTG-SNLDSEV 720
Query: 721 TVSASLVWSNGVREVRSPIVLF 736
SA+L+WS+G VRSPIV++
Sbjct: 721 PSSANLIWSDGTHNVRSPIVVY 728
BLAST of CmoCh04G012260 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 585.5 bits (1508), Expect = 6.0e-167
Identity = 322/705 (45.67%), Postives = 435/705 (61.70%), Query Frame = 0
Query: 36 MGSKPKDSSQPHLHHRAMLEEVVGSAFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEG 95
MG+ P+ P HH ++L+++VG+ ++ +V SYKRSFNGFA L++ E+Q++ +
Sbjct: 1 MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60
Query: 96 VVSVFPSKNYQLQTTRSWDFINFPRDVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGP 155
VVSVFPSK+++L TTRSWDF+ F R ++ES++IVGV+D+GIWP+S SFDD+G GP
Sbjct: 61 VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120
Query: 156 PPPKWKGICESSANFRCNRKIIGARAYRAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLV 215
PP KWKG C+ F CN K+IGAR Y A D GHGTH AST AG+ V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180
Query: 216 DNASLYGFGLGLARGGVPSARIAVYKVCWERGCSDANILAAFDDAIADGVDILSISIGGR 275
AS YG G ARGGVPSARIA YKVC+ R C+D +ILAAFDDAIADGVD++SISI
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCFNR-CNDVDILAAFDDAIADGVDVISISISAD 240
Query: 276 RVRPFFENGIAIGAFQAMRNGILVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAH 335
V +AIG+F AM GI+ + SAGN+GP+ + +++PWM++VAA+ DR F
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300
Query: 336 VQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGK 395
V LGNG G+++N F+L G ++P+VY N+ + + +C VD LV+GK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYG---QNVSRNCSQAQAGYCSSGCVDSELVKGK 360
Query: 396 IVLCDS-SNVSFPSLNGAVGVVMESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAP 455
IVLCD L GA+GV++++T P PAS I+ YI S P
Sbjct: 361 IVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPP 420
Query: 456 TATIFRSVNVNDTNAPVAAAFSSRGPNTISPNILKPDLSAPGVNILAAW-PQNVPISHGH 515
A I R+ + D AP +FSSRGP+ + N+LKPD+SAPG+ ILAA+ P P S +
Sbjct: 421 QAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLN 480
Query: 516 P---LSSPYNFASGTSMACPHATAIAAYVKTFSPWWSPAAIKSALMTTASPMDARFSQGA 575
P S Y+ SGTSMACPH +AAYVK+F P WSP+AIKSA+MTTA+PM+ + +
Sbjct: 481 PEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQ 540
Query: 576 EFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIPNITRAQLQQITGEDIICTDSNTGR 635
EFAYGSGQINP KA +PGLVY DY++ LC L +G+++ C++ +
Sbjct: 541 EFAYGSGQINPTKASDPGLVYEVETEDYLKMLCA-EGFDSTTLTTTSGQNVTCSERTEVK 600
Query: 636 DLDLNYPSFALSMGRGQSLRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTF 695
DLNYP+ + V F RT+TNV STY+A+V P LQ ++P L F
Sbjct: 601 --DLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVV-PLQPELQISIEPEILRF 660
Query: 696 HGIGDTKSFTLGVQGWLSATDPATVSASLVWSNGVREVRSPIVLF 736
+ + KSF + + G D + VS+S+VWS+G VRSPIV +
Sbjct: 661 GFLEEKKSFVVTISG-KELKDGSFVSSSVVWSDGSHSVRSPIVAY 689
BLAST of CmoCh04G012260 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 583.2 bits (1502), Expect = 3.0e-166
Identity = 334/747 (44.71%), Postives = 456/747 (61.04%), Query Frame = 0
Query: 5 ALRVVFLNLVCTLLISASASNG---DGRKIYIVYMGSKP-KDSSQPHLHHRAMLEEVVGS 64
A+ L+ + LL+ + AS G +++YIVYMG+ P + P HH ++L++V G
Sbjct: 4 AVSYCLLSCIFALLVVSFASAGKDDQDKQVYIVYMGALPSRVDYMPMSHHTSILQDVTGE 63
Query: 65 AFSAESIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP- 124
+ + +V +YKRSFNGFA +LTE E + +A+ + VVSVFPSKN LQTT SW+F+
Sbjct: 64 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 123
Query: 125 -RDVPRLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIG 184
+ R PL ES+ I+GV+D+GI+P+S SF +G GPPP KWKG+C+ NF CN K+IG
Sbjct: 124 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 183
Query: 185 ARAY--RAEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSAR 244
AR Y + EG P + D +GHG+H AS AG+ V + S YG G G RGGVP+AR
Sbjct: 184 ARYYTPKLEGFPESAR-----DNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAAR 243
Query: 245 IAVYKVCWERG---CSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAM 304
IAVYKVC + G C+ ILAAFDDAIAD VDI+++S+G V F E+ +AIGAF AM
Sbjct: 244 IAVYKVC-DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAM 303
Query: 305 RNGILVSNSAGNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFD 364
GIL N AGN+GP T SMAPW+ +VAA+ ++R F V LGNG G ++N FD
Sbjct: 304 AKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFD 363
Query: 365 LGGKQYPLVYAGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSS-NVSFPSLNGA 424
L GK+YPLVY + D+S + FC +D V+GKIVLCD+ N GA
Sbjct: 364 LNGKKYPLVYG---KSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGA 423
Query: 425 VGVVMESTASWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVA 484
V ++ + F P S + N + Y+ S P A + +S + + APV
Sbjct: 424 VASIVRNPYE-DAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVV 483
Query: 485 AAFSSRGPNTISPNILKPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHA 544
A++SSRGPN + +ILKPD++APG ILAA+ VP S Y SGTSM+CPH
Sbjct: 484 ASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSESDTRHVKYTVISGTSMSCPHV 543
Query: 545 TAIAAYVKTFSPWWSPAAIKSALMTTASPMDARFSQG---AEFAYGSGQINPLKAVNPGL 604
+AAY+KTF P WSP+ I+SA+MTTA PM+A S AEFAYG+G ++P+ A++PGL
Sbjct: 544 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 603
Query: 605 VYNASESDYVEYLCGIPNITRAQLQQITGEDIICTDSNT-GRDLDLNYPSFALSMGRGQS 664
VY A++SD++ +LCG N T +L+ I+G+ CT T +LNYPS + + +
Sbjct: 604 VYEANKSDHITFLCGF-NYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKP 663
Query: 665 LRVFFPRTLTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLS 724
+V F RT+TNV +TY+A V G L+ KV P L+ + + KSFT+ V G
Sbjct: 664 FKVTFRRTVTNVGRPNATYKAKVV---GSKLKVKVVPAVLSLKSLYEKKSFTVTVSG-AG 723
Query: 725 ATDPATVSASLVWSNGVREVRSPIVLF 736
VSA L+WS+GV VRSPIV++
Sbjct: 724 PKAENLVSAQLIWSDGVHFVRSPIVVY 735
BLAST of CmoCh04G012260 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 581.6 bits (1498), Expect = 8.6e-166
Identity = 326/730 (44.66%), Postives = 444/730 (60.82%), Query Frame = 0
Query: 13 LVCTLLISASASNGDGRKIYIVYMGSKPKDSSQ-PHLHHRAMLEEVVGSAFSAESIVYSY 72
L+ LL S SA + ++YIVYMGS + P H ++L++V G + +V SY
Sbjct: 14 LLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSY 73
Query: 73 KRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFP--RDVPRLPLQE 132
KRSFNGFA +LTE E +A EGVVSVFP+K QL TT SWDF+ ++ R E
Sbjct: 74 KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 133
Query: 133 SNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYRAEGPPP 192
S+ I+GV+DTGIWP+S SF D+G GPPP KWKG+C NF CN K+IGAR Y +EG
Sbjct: 134 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTSEGTR- 193
Query: 193 PTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVCWERGCS 252
DTSGHGTH AST AG+ V + S +G G G RGGVP++RIA YKVC + GCS
Sbjct: 194 --------DTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 253
Query: 253 DANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSAGNDGPN 312
+L++FDDAIADGVD+++ISIG + F ++ IAIGAF AM GIL +SAGN GP
Sbjct: 254 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 313
Query: 313 WYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVYAGDVPN 372
T +APW+ +VAA+T +R F V LGNG G ++N FD+ GK+YPLVY +
Sbjct: 314 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 373
Query: 373 IGGGFDSSISRFCDVNSVDRNLVRGKIVLCDS-SNVSFPSLNGAVGVVMESTASWHLIGT 432
D+ + C ++++ V+GKI++C S GA+ ++ +S + T
Sbjct: 374 --SACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIAIIDKSPRP-DVAFT 433
Query: 433 FPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPNTISPNIL 492
LPAS + YI S +P A + ++ + + +PV A+FSSRGPNTI+ +IL
Sbjct: 434 HHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDIL 493
Query: 493 KPDLSAPGVNILAAWPQNVPISHGHPLSSPYNFASGTSMACPHATAIAAYVKTFSPWWSP 552
KPD++APGV ILAA+ N S Y+ SGTSMACPH +AAYVKTF P WSP
Sbjct: 494 KPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSP 553
Query: 553 AAIKSALMTTASPMDA--RFSQGAEFAYGSGQINPLKAVNPGLVYNASESDYVEYLCGIP 612
+ I+SA+MTTA P+ A R EFAYG+G ++P+ A+NPGLVY ++D++ +LCG+
Sbjct: 554 SMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGM- 613
Query: 613 NITRAQLQQITGEDIICTDSNTGRDLDLNYPSFALSM-GRGQSLRVFFPRTLTNVEATVS 672
N T L+ I+G+ + C+ N +LNYPS + + G + V F RTLTNV S
Sbjct: 614 NYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNS 673
Query: 673 TYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVSASLVWSNGV 732
TY++ V + G L KV P L F + + +SF++ V G SA+L+WS+G
Sbjct: 674 TYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTG-SDVDSEVPSSANLIWSDGT 729
Query: 733 REVRSPIVLF 736
VRSPIV++
Sbjct: 734 HNVRSPIVVY 729
BLAST of CmoCh04G012260 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-165
Identity = 331/739 (44.79%), Postives = 453/739 (61.30%), Query Frame = 0
Query: 9 VFLNLVCTLLISASASNGD--GRKIYIVYMGSKP-KDSSQPHLHHRAMLEEVVGSAFSAE 68
+F +L+ L S SA D +++YIVY+GS P ++ P H ++L+E+ G +
Sbjct: 10 LFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESLIEN 69
Query: 69 SIVYSYKRSFNGFAVKLTEEEAQRMAAKEGVVSVFPSKNYQLQTTRSWDFINFPRDV--P 128
+V SYK+SFNGFA +LTE E +R+A E VVSVFPS+ +LQTT SW+F+ +
Sbjct: 70 RLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEGIKTK 129
Query: 129 RLPLQESNIIVGVLDTGIWPDSLSFDDQGLGPPPPKWKGICESSANFRCNRKIIGARAYR 188
R ES+ I+GV+D+GI+P+S SF DQG GPPP KWKG C NF CN K+IGAR Y
Sbjct: 130 RTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGARDYT 189
Query: 189 AEGPPPPTQAWGPIDTSGHGTHVASTVAGSLVDNASLYGFGLGLARGGVPSARIAVYKVC 248
A+ T D SGHGTH AS AG+ V N++ YG G G ARGGVP+ARIAVYKVC
Sbjct: 190 AKSKANQTAR----DYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC 249
Query: 249 WERGCSDANILAAFDDAIADGVDILSISIGGRRVRPFFENGIAIGAFQAMRNGILVSNSA 308
GC +++AFDDAIADGVD++SISI + PF E+ IAIGAF AM G+L N+A
Sbjct: 250 DNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAA 309
Query: 309 GNDGPNWYTTGSMAPWMLSVAANTIDRNFAAHVQLGNGNVYQGVAINLFDLGGKQYPLVY 368
GN+GP T S APW+ SVAA+ +R F A V LG+G + G ++N +D+ G YPLVY
Sbjct: 310 GNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVY 369
Query: 369 AGDVPNIGGGFDSSISRFCDVNSVDRNLVRGKIVLCDSSNVSFPSLN-GAVG-VVMESTA 428
D +R C+ +D LV+GKIVLCDS+ + GAVG +V
Sbjct: 370 GKSAALSTCSVDK--ARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEP 429
Query: 429 SWHLIGTFPLPASHFNPPSANPIRLYITSNPAPTATIFRSVNVNDTNAPVAAAFSSRGPN 488
I +FP+ S + + Y+ S P AT+ +S +++ AP+ A+FSSRGP+
Sbjct: 430 DRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPS 489
Query: 489 TISPNILKPDLSAPGVNILAAW-PQNVPI-SHGHPLSSPYNFASGTSMACPHATAIAAYV 548
+I +ILKPD++APGV ILAA+ P + P S Y+ SGTSMACPH +AAYV
Sbjct: 490 SIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYV 549
Query: 549 KTFSPWWSPAAIKSALMTTASPMDARFS--QGAEFAYGSGQINPLKAVNPGLVYNASESD 608
KTF P WSP+ I+SA+MTTA PM+A S EFAYGSG ++P+ A+NPGLVY +++D
Sbjct: 550 KTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKAD 609
Query: 609 YVEYLCGIPNITRAQLQQITGEDIICT-DSNTGRDLDLNYPSFALSMGRGQSLRVFFPRT 668
++ +LCG+ N T L+ I+G++ CT + + +LNYP+ + + + + F RT
Sbjct: 610 HINFLCGL-NYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRT 669
Query: 669 LTNVEATVSTYRATVYSPPGLNLQFKVDPPALTFHGIGDTKSFTLGVQGWLSATDPATVS 728
+TNV STY A V PG L KV P L+ + + +SF + V T VS
Sbjct: 670 VTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT-KQPVS 729
Query: 729 ASLVWSNGVREVRSPIVLF 736
A+L+WS+G VRSPI+++
Sbjct: 730 ANLIWSDGTHNVRSPIIVY 738
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 1.3e-235 | 58.61 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 4.8e-169 | 45.23 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FIG2 | 2.6e-167 | 43.94 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9STF7 | 4.2e-165 | 44.71 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
| Q8L7D2 | 1.2e-164 | 44.66 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1GX71 | 0.0e+00 | 98.64 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458261 PE=3 SV=1 | [more] |
| A0A6J1IMT3 | 0.0e+00 | 90.48 | cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1 | [more] |
| A0A6J1IP33 | 0.0e+00 | 90.86 | cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476976 PE=3 SV=1 | [more] |
| K7NBW1 | 3.4e-250 | 61.25 | Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1 | [more] |
| A0A6J1CBL9 | 8.3e-249 | 60.11 | LOW QUALITY PROTEIN: cucumisin-like OS=Momordica charantia OX=3673 GN=LOC1110101... | [more] |
