Homology
BLAST of CmoCh04G012250 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 990.7 bits (2560), Expect = 8.8e-288
Identity = 501/734 (68.26%), Postives = 582/734 (79.29%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCS-VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 67
MSS LI L F +LF S LAS S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
Query: 68 GSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGF 127
GSTF P+SVL+TY RSFNGF V LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDF+GF
Sbjct: 61 GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
Query: 128 PQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKIIG 187
P V R Q+ESNIVVGVLD+GIWPESPSF+D+GF PP KW+G C+ S NF CNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
Query: 188 ARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIA 247
AR+YHIG+ I+P +VN PRDTNGHGTHTAS AGGLVSQA+LY LGLGTARGG P ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
Query: 248 VYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGIL 307
YK+CW GCSD DILAA+DDAIADGVD ISLSVG + PR YF D IAIGSFHA+++GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
Query: 308 TSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQ 367
TSNSAGN GP TT SLSPWLLSVAA+TMDRKFVT+V++G+G+ FQGVSINTF+
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ--Y 360
Query: 368 YPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEA-IVPPSLFSSLGAINGVIM 427
YPLV G D+PN GF +S SR+C SVNP ++GK+++CEA P F SL GV+M
Sbjct: 361 YPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
Query: 428 QSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
S TRD A SYPLP++++ D++ R+ +P A FKS + SAPV+ SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480
Query: 488 GPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
GPN ++KPD+SGPGVEIL AWP VAP+ ++ R++L+NIISGTSMSC HITG+A
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIAT 540
Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
YVKT+NPT SPAAIKSALMTTASPMNA + QAEFAYG+GHVNPLKAV PGLVYDA+E++
Sbjct: 541 YVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESD 600
Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
YVKFLCGQGY+T VRRITGD SACTSGN GRVWDLN PSF LSV+ S+ NQYF RTL
Sbjct: 601 YVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSP-SQTFNQYFNRTL 660
Query: 668 TNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 727
T+VA STYRA ISAP+GL I+VNP VLSFN +G+RKSF LTV G++ +VSA LVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720
Query: 728 DGVHSVRSPITIST 739
DGVH VRSPITI++
Sbjct: 721 DGVHYVRSPITITS 729
BLAST of CmoCh04G012250 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 601.3 bits (1549), Expect = 1.5e-170
Identity = 329/744 (44.22%), Postives = 466/744 (62.63%), Query Frame = 0
Query: 9 SSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQ 68
+S +L+ L + SV A T DD+++YIVYMGS P+S H++ +L++
Sbjct: 6 ASSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQE 65
Query: 69 VVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFM 128
V G + ++ +Y RSFNGF LTE E + +A GVVSVF N+ L TT SWDFM
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125
Query: 129 GFPQNV--DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNR 188
G + + R P +ES+ ++GV+DSGI PES SF+D GFG PP KW+G C NF+CN
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNN 185
Query: 189 KIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPS 248
K+IGAR Y RD +GHGTHTAS AG V AS + +G GT RGG P+
Sbjct: 186 KLIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 245
Query: 249 ARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMK 308
+R+A YK+C GCS +L+AFDDAIADGVD I++S+G + +DPIAIG+FHAM
Sbjct: 246 SRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 305
Query: 309 KGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNT 368
KG+LT NSAGN GPK + + ++PW+L+VAA+T +R FVT+V LG+G+ G S+N +
Sbjct: 306 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 365
Query: 369 MVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAING 428
YPLVYG + + C + V+ +V+GK+L+C + S+GA+ G
Sbjct: 366 KGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-G 425
Query: 429 VIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASF 488
+I ++ D A +PLPAA + +D ++ + T +P A+ K+ A + ++PVIASF
Sbjct: 426 LIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 485
Query: 489 SSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITG 548
SSRGPN++A ILKPD++ PGVEIL A+ P ++ D+R Y+++SGTSMSC H+ G
Sbjct: 486 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ-DDTRHVKYSVLSGTSMSCPHVAG 545
Query: 549 VAVYVKTFNPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYD 608
VA YVKTFNP SP+ I+SA+MTTA P+NA+ + EFAYG+GHV+P+ A NPGLVY+
Sbjct: 546 VAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYE 605
Query: 609 ADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQY 668
D+++++ FLCG Y + +++ I+G+ C+ +LN PS + ++
Sbjct: 606 LDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVT 665
Query: 669 FTRTLTNVASSGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRR 728
F RTLTNV + STY + + A G L + + P VLSF + E++SF +TV G+ S
Sbjct: 666 FNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEV 725
Query: 729 LVSACLVWSDGVHSVRSPITISTS 740
SA L+WSDG H+VRSPI + TS
Sbjct: 726 PSSANLIWSDGTHNVRSPIVVYTS 730
BLAST of CmoCh04G012250 vs. ExPASy Swiss-Prot
Match:
Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)
HSP 1 Score: 597.0 bits (1538), Expect = 2.8e-169
Identity = 332/739 (44.93%), Postives = 467/739 (63.19%), Query Frame = 0
Query: 13 ILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL----HHRAMLEQVVGS 72
+L+ +F L S +++ D D+++YIVYMG+ PS HH ++L+ V G
Sbjct: 9 LLSCIFALLVVSF--ASAGKDDQDKQVYIVYMGAL---PSRVDYMPMSHHTSILQDVTGE 68
Query: 73 TFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ 132
+ ++ Y RSFNGF LTE E + +AS + VVSVF ++N +L TT SW+FMG +
Sbjct: 69 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 128
Query: 133 --NVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIG 192
R P +ES+ ++GV+DSGI+PES SF+ GFG PP KW+G C+ +NF+CN K+IG
Sbjct: 129 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 188
Query: 193 ARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIA 252
AR Y P+ S RD GHG+HTASI AG V S Y LG GT RGG P+ARIA
Sbjct: 189 ARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 248
Query: 253 VYKIC--WKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKG 312
VYK+C C+ ILAAFDDAIAD VD I++S+G+ + D +AIG+FHAM KG
Sbjct: 249 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 308
Query: 313 ILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMV 372
ILT N AGN GP+ T S++PWL +VAA+ M+R F+T+V LG+G+ G S+N+F+
Sbjct: 309 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 368
Query: 373 YQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVI 432
+YPLVYG +R S + +C ++ ++V+GK++LC+ P ++GA+ ++
Sbjct: 369 KKYPLVYGKSASSR-CDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 428
Query: 433 MQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSS 492
++ D A + P +++S D + + T P A KS + APV+AS+SS
Sbjct: 429 -RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSS 488
Query: 493 RGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVA 552
RGPN L + ILKPD++ PG EIL A+ P P +E D+R Y +ISGTSMSC H+ GVA
Sbjct: 489 RGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE-SDTRHVKYTVISGTSMSCPHVAGVA 548
Query: 553 VYVKTFNPTLSPAAIKSALMTTASPMNASL---HSQAEFAYGAGHVNPLKAVNPGLVYDA 612
Y+KTF+P SP+ I+SA+MTTA PMNAS + AEFAYGAGHV+P+ A++PGLVY+A
Sbjct: 549 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 608
Query: 613 DENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVW-DLNLPSFALSVAAGSKALNQY 672
++++++ FLCG Y +R I+GD+S+CT + +LN PS + V +G+K
Sbjct: 609 NKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQV-SGTKPFKVT 668
Query: 673 FTRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLV 732
F RT+TNV +TY+A + K L++ V P VLS + E+KSF +TV G + LV
Sbjct: 669 FRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLV 728
Query: 733 SACLVWSDGVHSVRSPITI 737
SA L+WSDGVH VRSPI +
Sbjct: 729 SAQLIWSDGVHFVRSPIVV 734
BLAST of CmoCh04G012250 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 595.1 bits (1533), Expect = 1.1e-168
Identity = 329/733 (44.88%), Postives = 457/733 (62.35%), Query Frame = 0
Query: 17 LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 76
L+ L +L+S S D+D ++YIVYMGS P+S H+ ++L+QV G +
Sbjct: 10 LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69
Query: 77 QSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 136
++ +Y RSFNGF LTE E IA EGVVSVF N+ LHTT SWDFMG + N
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129
Query: 137 DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIGARAY 196
R +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C NF+CN K+IGAR Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 197 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 256
RDT+GHGTHTAS AG V S + +G GT RGG P++RIA YK+
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249
Query: 257 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 316
C GCS +L++FDDAIADGVD I++S+G P + DPIAIG+FHAM KGILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309
Query: 317 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLV 376
AGN GPK +T + ++PW+ +VAA+T +R F+T+V LG+G+ G S+N F+ +YPLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369
Query: 377 YGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTR 436
YG + + C +N +V+GK+L+C + S+GAI +I +S
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRP 429
Query: 437 DNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSL 496
D A ++ LPA+ + +D ++ + +P A K+ + ++PVIASFSSRGPN++
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489
Query: 497 ANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTF 556
A ILKPD++ PGVEIL A+ P +E D+R Y++ SGTSM+C H+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549
Query: 557 NPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 616
P SP+ I+SA+MTTA P+ A + EFAYGAGHV+P+ A+NPGLVY+ D+ +++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609
Query: 617 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 676
FLCG Y + ++ I+GD C+ N +LN PS + ++ + F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669
Query: 677 ASSGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLVSACLVW 736
+ STY++ + A G L I V P VL F + E++SF++TV G+ S SA L+W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 728
BLAST of CmoCh04G012250 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 592.4 bits (1526), Expect = 6.9e-168
Identity = 318/734 (43.32%), Postives = 462/734 (62.94%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
+S+PL L L F +++ + +YIVYMG+ + S HH ++L+++VG
Sbjct: 4 LSTPLYLICL---AFIFTRDVSANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVG 63
Query: 68 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127
+ ++ +Y RSFNGF L++ E+Q++ + + VVSVF ++++ L TTRSWDF+GF
Sbjct: 64 TIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFG 123
Query: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKIIGA 187
+ R ES+++VGV+DSGIWPES SF+D+GFG PP KW+G C+ F+CN K+IGA
Sbjct: 124 EKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGA 183
Query: 188 RAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAV 247
R Y+ +S RD GHGTHTAS AG V AS Y L GTARGG PSARIA
Sbjct: 184 RFYN-------KFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAA 243
Query: 248 YKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILT 307
YK+C+ C+D DILAAFDDAIADGVD IS+S+ + + +AIGSFHAM +GI+T
Sbjct: 244 YKVCFNR-CNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIIT 303
Query: 308 SNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQY 367
+ SAGN GP + ++SPW+++VAA+ DR+F+ RV LG+G+ G+S+NTFN ++
Sbjct: 304 AGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKF 363
Query: 368 PLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQS 427
P+VYG +V +R ++ + YC V+ E V+GK++LC+ + GAI ++ +
Sbjct: 364 PIVYGQNV-SRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNT 423
Query: 428 GTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGP 487
D+A P PA+ + +D +I + P A ++ D AP + SFSSRGP
Sbjct: 424 LLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGP 483
Query: 488 NSLANHILKPDLSGPGVEILGAWPPVA---PIAEVKDSRSSLYNIISGTSMSCSHITGVA 547
+ + ++LKPD+S PG+EIL A+ PVA +D RS Y+++SGTSM+C H+ GVA
Sbjct: 484 SFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVA 543
Query: 548 VYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADEN 607
YVK+F+P SP+AIKSA+MTTA+PMN + + EFAYG+G +NP KA +PGLVY+ +
Sbjct: 544 AYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETE 603
Query: 608 EYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRT 667
+Y+K LC +G+D+ + +G N C+ V DLN P+ V++ N F RT
Sbjct: 604 DYLKMLCAEGFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSS-LDPFNVTFKRT 663
Query: 668 LTNVASSGSTYRAT-ISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSR--RLVSAC 727
+TNV STY+A+ + L+I++ P +L F + E+KSF +T+ G + VS+
Sbjct: 664 VTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSS 722
Query: 728 LVWSDGVHSVRSPI 735
+VWSDG HSVRSPI
Sbjct: 724 VVWSDGSHSVRSPI 722
BLAST of CmoCh04G012250 vs. ExPASy TrEMBL
Match:
A0A6J1H1P2 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1)
HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
Query: 68 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127
STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
Query: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 187
QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 180
Query: 188 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 247
AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY
Sbjct: 181 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 240
Query: 248 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 307
KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS
Sbjct: 241 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 300
Query: 308 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 367
NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP
Sbjct: 301 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 360
Query: 368 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 427
LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG
Sbjct: 361 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 420
Query: 428 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 487
TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN
Sbjct: 421 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 480
Query: 488 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 547
SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK
Sbjct: 481 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 540
Query: 548 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 607
TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 600
Query: 608 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 667
FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV
Sbjct: 601 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 660
Query: 668 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 727
ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV
Sbjct: 661 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 720
Query: 728 HSVRSPITISTSR 741
HSVRSPITISTSR
Sbjct: 721 HSVRSPITISTSR 733
BLAST of CmoCh04G012250 vs. ExPASy TrEMBL
Match:
A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)
HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 609/736 (82.74%), Postives = 661/736 (89.81%), Query Frame = 0
Query: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRA 60
Query: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS
Sbjct: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
Query: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN 180
WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN
Sbjct: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN 180
Query: 181 RKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAP 240
RK+IG RAYHIG+ IAP +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG P
Sbjct: 181 RKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVP 240
Query: 241 SARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAM 300
SARIA YK+CW+ GC DADILAAFDDAIADGVD +SLSVGS+KPRPYF+DPIAIGSFHA+
Sbjct: 241 SARIAAYKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAI 300
Query: 301 KKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFN 360
K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G +QG+SINTFN
Sbjct: 301 KNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFN 360
Query: 361 TMVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAIN 420
M Q+PLVY GD+PN GF S SRYC +NSVNP GK+LLC++++PPS F S GAI
Sbjct: 361 -MNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIV 420
Query: 421 GVIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIAS 480
G++MQ+ TRDNA SYP PAA++ D I ++R+ R T+ P A FKS A QD SAPV+ S
Sbjct: 421 GILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVS 480
Query: 481 FSSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHIT 540
FSSRGPN+L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+T
Sbjct: 481 FSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLT 540
Query: 541 GVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDA 600
GVAVYVKTFNPTLSPAAIKSALMTTASPM+A HSQAEFAYGAGHVNPLKA+NPGLVYDA
Sbjct: 541 GVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDA 600
Query: 601 DENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYF 660
+E++YV FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLN PSFA+S+ +G++ +NQYF
Sbjct: 601 NESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSI-SGTRTVNQYF 660
Query: 661 TRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSAC 720
TRTLTNVA GSTYRA ISAP+GL ITVNP LSFN IG+RKSF LTV GTVS+ + SA
Sbjct: 661 TRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSAS 720
Query: 721 LVWSDGVHSVRSPITI 737
L WSDGVHSVRSPIT+
Sbjct: 721 LEWSDGVHSVRSPITV 734
BLAST of CmoCh04G012250 vs. ExPASy TrEMBL
Match:
A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)
HSP 1 Score: 1053.5 bits (2723), Expect = 4.1e-304
Identity = 526/728 (72.25%), Postives = 610/728 (83.79%), Query Frame = 0
Query: 10 SPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 69
S +I L+ L+L S+LAS S DD RKIYIVYMG+KL+DP SAHLHHRAMLE+VVGST
Sbjct: 8 SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGST 67
Query: 70 FDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 129
F P+SV+YTY RSFNGF V LTEEEAQ+IA+KEGVVSVFLNQ NH+HTT SWDF+GFPQ
Sbjct: 68 FAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQA 127
Query: 130 VDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKIIGARA 189
V R+ Q+ESNIVVGVLDSGIWPESPSF D+GFG PS W+G CQ S NFSCN KIIGARA
Sbjct: 128 VARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNNKIIGARA 187
Query: 190 YHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYK 249
Y +G ++ P +V+SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIA YK
Sbjct: 188 YRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 247
Query: 250 ICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSN 309
ICW GCSDADILAAFDDAIADGV ISLSVG S+ RPYF+DPIAIG+FHAMK GILTSN
Sbjct: 248 ICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILTSN 307
Query: 310 SAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPL 369
SAGNEG K TTTSLSPWLLSVAA+T DRKFVT V+LG+G+ +QG +INTF+ M QYPL
Sbjct: 308 SAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFD-MNNQYPL 367
Query: 370 VYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGT 429
VY G+VPN GF S SR+C NSVNP V+GK+LLC++I+P S+FSS GAI GV+MQ G
Sbjct: 368 VYAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQ-GY 427
Query: 430 RDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNS 489
DNA S+ +PA ++ QD ++R+ T TP A +D SAPV+ SFSSRGPN+
Sbjct: 428 TDNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPNA 487
Query: 490 LANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKT 549
L N +LKPD++GPGVEIL AWPP+API+ V++SRS+L+NIISGTSMSC H++ VAVYVKT
Sbjct: 488 LTNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVKT 547
Query: 550 FNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKF 609
FNP+ SPAAIKSALMTTASPM A L+S+AEFAYG+GHVNPLKAVNPGLVYDAD+++YVKF
Sbjct: 548 FNPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVKF 607
Query: 610 LCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVA 669
LCGQGY+TAM+RRITGDNSACTS NIGRVWDLN PSFAL+V +G ++LNQYFTRTLTNVA
Sbjct: 608 LCGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAV-SGYQSLNQYFTRTLTNVA 667
Query: 670 SSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGVH 729
S STYRATIS P+GL ITVNP VLSF+ G+RKSF LTV G+VS+ +VS LVWSDGVH
Sbjct: 668 SQASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGVH 727
Query: 730 SVRSPITI 737
SVRSP+ +
Sbjct: 728 SVRSPLVV 732
BLAST of CmoCh04G012250 vs. ExPASy TrEMBL
Match:
K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)
HSP 1 Score: 1016.1 bits (2626), Expect = 7.2e-293
Identity = 513/732 (70.08%), Postives = 597/732 (81.56%), Query Frame = 0
Query: 6 SDMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQV 65
S MSS L+ L+FL+LFCS+L S+S S+DD RKIYIVYMGSKL+D +SAHL+HRAMLE+V
Sbjct: 4 STMSS-LVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEV 63
Query: 66 VGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMG 125
VGSTF P+SV+YTY RSFNGF V LTEEEA +IA+KEGVVSVF ++ NHLHTTRSWDF+G
Sbjct: 64 VGSTFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLG 123
Query: 126 FPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKII 185
QNV RV Q+ESNIVVGV DSGIWPE+PSFNDDGFG P+ WRG CQ S NF CNRKII
Sbjct: 124 ISQNVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKII 183
Query: 186 GARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARI 245
GARAY ++ P +V SPRDT+GHGTHTAS VAG LVSQASLY LG+GTARGG P ARI
Sbjct: 184 GARAYR-SSTLPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARI 243
Query: 246 AVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGI 305
AVYKICW GCSDADILAAFDDAIADGVD ISLSVG P+PY + IAIGSFHAMK+GI
Sbjct: 244 AVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGI 303
Query: 306 LTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVY 365
LTSNSAGN GPK T TSLSPWL +VAA++ DRKFVT+V LG+G +QGVSINTF+ M
Sbjct: 304 LTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFD-MRN 363
Query: 366 QYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIM 425
QYPL+Y G+ P+ GF S SRYC ++SV+P VRGK+LLC++ P++F+S G GV+M
Sbjct: 364 QYPLIYAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLM 423
Query: 426 QSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 485
QS TRD+A SYPLPA+++ I R+ T P A FKS +D SAPV+ SFSSR
Sbjct: 424 QSNTRDHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSR 483
Query: 486 GPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 545
GPN + + ILKPD + PGVEIL AWPPVAPI+ V+DSRS+LYNIISGTSMSC H+T +AV
Sbjct: 484 GPNYVTHDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAV 543
Query: 546 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 605
++KTF P+ SPAAIKSALMTTASPMNA +S AEFAYG+GHVNPLKAV+PGLVYDA E++
Sbjct: 544 HIKTFYPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESD 603
Query: 606 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 665
YVKFLCG+GY TAMVR TGDNSACTSGNIGRVWDLN PSFALS++ S+ NQ F RTL
Sbjct: 604 YVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISR-SQTANQSFRRTL 663
Query: 666 TNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 725
TNV S STYRA+ISAP+GL I+VNP VLSFN IG++KSF LTV GTVS+ +VSA LVWS
Sbjct: 664 TNVVSGASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWS 723
Query: 726 DGVHSVRSPITI 737
DG H+VRSPIT+
Sbjct: 724 DGSHNVRSPITV 731
BLAST of CmoCh04G012250 vs. ExPASy TrEMBL
Match:
A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)
HSP 1 Score: 993.8 bits (2568), Expect = 3.8e-286
Identity = 503/734 (68.53%), Postives = 584/734 (79.56%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCS-VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 67
MSS LI L F +LF S LAS S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60
Query: 68 GSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGF 127
GSTF P+SVL+TY RSFNGF V LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDF+GF
Sbjct: 61 GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120
Query: 128 PQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVS-NFSCNRKIIG 187
P V R Q+ESNIVVGVLD+GIWPESPSF+D+GF PP KW+G C+ S NF CNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180
Query: 188 ARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIA 247
AR+YHIG+ I+P +VN PRDTNGHGTHTAS AGGLVSQA+LY LGLGTARGG P ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240
Query: 248 VYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGIL 307
YK+CW GCSDADILAA+DDAIADGVD ISLSVG + PR YF D IAIGSFHA+++GIL
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300
Query: 308 TSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQ 367
TSNSAGN GP TT SLSPWLLSVAA+TMDRKFVT+V++G+G+ FQGVSINTF+
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFDNQ--Y 360
Query: 368 YPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEA-IVPPSLFSSLGAINGVIM 427
YPLV G D+PN GF +S SR+C NSV P+ ++GK+++CEA P F SL GV+M
Sbjct: 361 YPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLM 420
Query: 428 QSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSR 487
S TRD A SYPLP++++ D++ R+ +P A FKS + SAPV+ SFSSR
Sbjct: 421 TSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSR 480
Query: 488 GPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAV 547
GPN ++KPD+SGPGVEIL AWP VAP+ ++ R++L+NIISGTSMSC HITG+A
Sbjct: 481 GPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIAT 540
Query: 548 YVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENE 607
YVKT+NPT SPAAIKSALMTTASPMNA + QAEFAYG+GHVNPLKAV PGLVYDA+E++
Sbjct: 541 YVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESD 600
Query: 608 YVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTL 667
YVKFLCGQGY+T VRRITGD SACT GN GRVWDLN PSF LSV+ SK NQYF RTL
Sbjct: 601 YVKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSP-SKTFNQYFNRTL 660
Query: 668 TNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWS 727
T+VA STYRA ISAP+GL I+VNP VLSFN +G+RKSF LTV G++ +VSA LVWS
Sbjct: 661 TSVAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWS 720
Query: 728 DGVHSVRSPITIST 739
DGVHSVRSPITI++
Sbjct: 721 DGVHSVRSPITITS 729
BLAST of CmoCh04G012250 vs. NCBI nr
Match:
XP_022957349.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1465.3 bits (3792), Expect = 0.0e+00
Identity = 733/733 (100.00%), Postives = 733/733 (100.00%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
Query: 68 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127
STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
Query: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 187
QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 180
Query: 188 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 247
AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY
Sbjct: 181 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 240
Query: 248 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 307
KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS
Sbjct: 241 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 300
Query: 308 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 367
NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP
Sbjct: 301 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 360
Query: 368 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 427
LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG
Sbjct: 361 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 420
Query: 428 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 487
TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN
Sbjct: 421 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 480
Query: 488 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 547
SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK
Sbjct: 481 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 540
Query: 548 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 607
TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 600
Query: 608 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 667
FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV
Sbjct: 601 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 660
Query: 668 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 727
ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV
Sbjct: 661 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 720
Query: 728 HSVRSPITISTSR 741
HSVRSPITISTSR
Sbjct: 721 HSVRSPITISTSR 733
BLAST of CmoCh04G012250 vs. NCBI nr
Match:
KAG6600968.1 (hypothetical protein SDJN03_06201, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1294.3 bits (3348), Expect = 0.0e+00
Identity = 667/743 (89.77%), Postives = 676/743 (90.98%), Query Frame = 0
Query: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
MIRKGS+MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1 MIRKGSNMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
Query: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
MLEQVVGSTFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLN+NNHLHTTRS
Sbjct: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNENNHLHTTRS 120
Query: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ---VSNF 180
WDF+GF QNV RV Q+ESNIVVGV+DSGIWPESPSFND+ FG P W+G CQ +NF
Sbjct: 121 WDFLGFSQNVSRVNQVESNIVVGVMDSGIWPESPSFNDEAFGPAPPNWKGICQNNNNNNF 180
Query: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG
Sbjct: 181 SCNRKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARG 240
Query: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF
Sbjct: 241 GAPSARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSF 300
Query: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSIN 360
HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQ +
Sbjct: 301 HAMKKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQFL-FT 360
Query: 361 TFNTMVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLG 420
FN AIVPPSLFSSLG
Sbjct: 361 KFN---------------------------------------------AIVPPSLFSSLG 420
Query: 421 AINGVIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPV 480
AINGVIMQSGTRDNA SYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPV
Sbjct: 421 AINGVIMQSGTRDNATSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPV 480
Query: 481 IASFSSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCS 540
IASFSSRGPNSL NHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCS
Sbjct: 481 IASFSSRGPNSLTNHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCS 540
Query: 541 HITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLV 600
HITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLV
Sbjct: 541 HITGVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLV 600
Query: 601 YDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALN 660
YDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALN
Sbjct: 601 YDADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALN 660
Query: 661 QYFTRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLV 720
QYFTRTLTNVASSGSTYRATISAPKGLRITVNP VLSFNRIGERKSFNLTVGGTVSRRLV
Sbjct: 661 QYFTRTLTNVASSGSTYRATISAPKGLRITVNPSVLSFNRIGERKSFNLTVGGTVSRRLV 697
Query: 721 SACLVWSDGVHSVRSPITISTSR 741
SACLVWSDGVHSVRSPITISTSR
Sbjct: 721 SACLVWSDGVHSVRSPITISTSR 697
BLAST of CmoCh04G012250 vs. NCBI nr
Match:
XP_022957348.1 (cucumisin-like [Cucurbita moschata])
HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 609/736 (82.74%), Postives = 661/736 (89.81%), Query Frame = 0
Query: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRA
Sbjct: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRA 60
Query: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS
Sbjct: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
Query: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN 180
WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN
Sbjct: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN 180
Query: 181 RKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAP 240
RK+IG RAYHIG+ IAP +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG P
Sbjct: 181 RKLIGGRAYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVP 240
Query: 241 SARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAM 300
SARIA YK+CW+ GC DADILAAFDDAIADGVD +SLSVGS+KPRPYF+DPIAIGSFHA+
Sbjct: 241 SARIAAYKVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAI 300
Query: 301 KKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFN 360
K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G +QG+SINTFN
Sbjct: 301 KNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFN 360
Query: 361 TMVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAIN 420
M Q+PLVY GD+PN GF S SRYC +NSVNP GK+LLC++++PPS F S GAI
Sbjct: 361 -MNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIV 420
Query: 421 GVIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIAS 480
G++MQ+ TRDNA SYP PAA++ D I ++R+ R T+ P A FKS A QD SAPV+ S
Sbjct: 421 GILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVS 480
Query: 481 FSSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHIT 540
FSSRGPN+L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+T
Sbjct: 481 FSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLT 540
Query: 541 GVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDA 600
GVAVYVKTFNPTLSPAAIKSALMTTASPM+A HSQAEFAYGAGHVNPLKA+NPGLVYDA
Sbjct: 541 GVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDA 600
Query: 601 DENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYF 660
+E++YV FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLN PSFA+S+ +G++ +NQYF
Sbjct: 601 NESDYVSFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSI-SGTRTVNQYF 660
Query: 661 TRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSAC 720
TRTLTNVA GSTYRA ISAP+GL ITVNP LSFN IG+RKSF LTV GTVS+ + SA
Sbjct: 661 TRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSAS 720
Query: 721 LVWSDGVHSVRSPITI 737
L WSDGVHSVRSPIT+
Sbjct: 721 LEWSDGVHSVRSPITV 734
BLAST of CmoCh04G012250 vs. NCBI nr
Match:
KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 598/729 (82.03%), Postives = 649/729 (89.03%), Query Frame = 0
Query: 8 MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67
M S L+LNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1 MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
Query: 68 STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127
STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61 STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
Query: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 187
QNV RV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ SNFSCNRK+IG R
Sbjct: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
Query: 188 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 247
AYHIG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIA Y
Sbjct: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
Query: 248 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 307
K+CW+ GCSDADILAAFDDAIADGVD +SLSVGSSKPRPYFSDPIAIGSFHA+K GILTS
Sbjct: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
Query: 308 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 367
NSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G +QG+SINTFN M Q+P
Sbjct: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFN-MNLQHP 360
Query: 368 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 427
LVY GD+PN GF S SRYC +NSVNP GK+LLC++++PPS FSS GAI G++MQ+
Sbjct: 361 LVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAY 420
Query: 428 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 487
TRDNA SYP PAA++ D I ++R+ R T+ P A FKS A QD SAPV+ SFSSRGPN
Sbjct: 421 TRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPN 480
Query: 488 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 547
+L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVYVK
Sbjct: 481 ALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVK 540
Query: 548 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 607
TFNPTLSPAAIKSALMTTASPM+A HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++YV
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 600
Query: 608 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 667
FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S+ +G++ +NQYFTRTLTNV
Sbjct: 601 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSI-SGTRTVNQYFTRTLTNV 660
Query: 668 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 727
A GSTYRA ISAP+GL ITVNP LSFN IG+RKSF LTVGGTVS+ + SA L WSDGV
Sbjct: 661 ARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGV 720
Query: 728 HSVRSPITI 737
HSVRSPIT+
Sbjct: 721 HSVRSPITV 727
BLAST of CmoCh04G012250 vs. NCBI nr
Match:
XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 590/736 (80.16%), Postives = 648/736 (88.04%), Query Frame = 0
Query: 1 MIRKGSDMSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRA 60
M RKG M S L+ NLLFLALF SVLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRA
Sbjct: 1 MTRKGITMCSSLLFNLLFLALFRSVLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRA 60
Query: 61 MLEQVVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
MLEQVVGSTFDPQSVLYTY RSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRS
Sbjct: 61 MLEQVVGSTFDPQSVLYTYTRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRS 120
Query: 121 WDFMGFPQNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCN 180
WDF+GFPQNV RVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRG CQVSNF CN
Sbjct: 121 WDFLGFPQNVGRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCN 180
Query: 181 RKIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAP 240
RK+IG RAY IG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LG+GTARGG P
Sbjct: 181 RKLIGGRAYRIGRPILPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVP 240
Query: 241 SARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAM 300
SARIA YK+CWK GCSDADILAAFDDAIADGVD +SLSVGSS+PRPYF+DPIAIGSFHA+
Sbjct: 241 SARIAAYKVCWKDGCSDADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAI 300
Query: 301 KKGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFN 360
K GILTSNSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G ++G+SINTF+
Sbjct: 301 KNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFD 360
Query: 361 TMVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAIN 420
M QYPLVY GD+PN GF S SRYC KNSVNP GK+LLC++++P S+FSS GAI
Sbjct: 361 -MNLQYPLVYAGDIPNLGFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIA 420
Query: 421 GVIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIAS 480
G++MQ+ TRDNA SYP PAA++ D I ++R+ R T+ P A FKS A QD SAP++ S
Sbjct: 421 GILMQAYTRDNARSYPAPAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVS 480
Query: 481 FSSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHIT 540
FSSRGPN+L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+T
Sbjct: 481 FSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLT 540
Query: 541 GVAVYVKTFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDA 600
GVAVYVKTFNPTLSPAAIKSAL+TTASPM+A HSQAEFAYGAGHVNPLKA+NPGLVYDA
Sbjct: 541 GVAVYVKTFNPTLSPAAIKSALITTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDA 600
Query: 601 DENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYF 660
+E++YV FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S+ +G++ +NQYF
Sbjct: 601 NESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSI-SGTRTVNQYF 660
Query: 661 TRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSAC 720
TRTLTNVA GSTYRA ISAP+GL ITVNP LSFN IG+RKSF LTVGGTVS+ + SA
Sbjct: 661 TRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSAS 720
Query: 721 LVWSDGVHSVRSPITI 737
L WSDGVHSVRSPIT+
Sbjct: 721 LEWSDGVHSVRSPITV 734
BLAST of CmoCh04G012250 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-171
Identity = 329/744 (44.22%), Postives = 466/744 (62.63%), Query Frame = 0
Query: 9 SSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQ 68
+S +L+ L + SV A T DD+++YIVYMGS P+S H++ +L++
Sbjct: 6 ASSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQE 65
Query: 69 VVGSTFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFM 128
V G + ++ +Y RSFNGF LTE E + +A GVVSVF N+ L TT SWDFM
Sbjct: 66 VTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFM 125
Query: 129 GFPQNV--DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNR 188
G + + R P +ES+ ++GV+DSGI PES SF+D GFG PP KW+G C NF+CN
Sbjct: 126 GLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNN 185
Query: 189 KIIGARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPS 248
K+IGAR Y RD +GHGTHTAS AG V AS + +G GT RGG P+
Sbjct: 186 KLIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPA 245
Query: 249 ARIAVYKICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMK 308
+R+A YK+C GCS +L+AFDDAIADGVD I++S+G + +DPIAIG+FHAM
Sbjct: 246 SRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMA 305
Query: 309 KGILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNT 368
KG+LT NSAGN GPK + + ++PW+L+VAA+T +R FVT+V LG+G+ G S+N +
Sbjct: 306 KGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEM 365
Query: 369 MVYQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAING 428
YPLVYG + + C + V+ +V+GK+L+C + S+GA+ G
Sbjct: 366 KGKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-G 425
Query: 429 VIMQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASF 488
+I ++ D A +PLPAA + +D ++ + T +P A+ K+ A + ++PVIASF
Sbjct: 426 LIYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASF 485
Query: 489 SSRGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITG 548
SSRGPN++A ILKPD++ PGVEIL A+ P ++ D+R Y+++SGTSMSC H+ G
Sbjct: 486 SSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQ-DDTRHVKYSVLSGTSMSCPHVAG 545
Query: 549 VAVYVKTFNPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYD 608
VA YVKTFNP SP+ I+SA+MTTA P+NA+ + EFAYG+GHV+P+ A NPGLVY+
Sbjct: 546 VAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYE 605
Query: 609 ADENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQY 668
D+++++ FLCG Y + +++ I+G+ C+ +LN PS + ++
Sbjct: 606 LDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVT 665
Query: 669 FTRTLTNVASSGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRR 728
F RTLTNV + STY + + A G L + + P VLSF + E++SF +TV G+ S
Sbjct: 666 FNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEV 725
Query: 729 LVSACLVWSDGVHSVRSPITISTS 740
SA L+WSDG H+VRSPI + TS
Sbjct: 726 PSSANLIWSDGTHNVRSPIVVYTS 730
BLAST of CmoCh04G012250 vs. TAIR 10
Match:
AT3G46850.1 (Subtilase family protein )
HSP 1 Score: 597.0 bits (1538), Expect = 2.0e-170
Identity = 332/739 (44.93%), Postives = 467/739 (63.19%), Query Frame = 0
Query: 13 ILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL----HHRAMLEQVVGS 72
+L+ +F L S +++ D D+++YIVYMG+ PS HH ++L+ V G
Sbjct: 9 LLSCIFALLVVSF--ASAGKDDQDKQVYIVYMGAL---PSRVDYMPMSHHTSILQDVTGE 68
Query: 73 TFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ 132
+ ++ Y RSFNGF LTE E + +AS + VVSVF ++N +L TT SW+FMG +
Sbjct: 69 SSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKE 128
Query: 133 --NVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIG 192
R P +ES+ ++GV+DSGI+PES SF+ GFG PP KW+G C+ +NF+CN K+IG
Sbjct: 129 GKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNKLIG 188
Query: 193 ARAYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIA 252
AR Y P+ S RD GHG+HTASI AG V S Y LG GT RGG P+ARIA
Sbjct: 189 ARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIA 248
Query: 253 VYKIC--WKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKG 312
VYK+C C+ ILAAFDDAIAD VD I++S+G+ + D +AIG+FHAM KG
Sbjct: 249 VYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKG 308
Query: 313 ILTSNSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMV 372
ILT N AGN GP+ T S++PWL +VAA+ M+R F+T+V LG+G+ G S+N+F+
Sbjct: 309 ILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNG 368
Query: 373 YQYPLVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVI 432
+YPLVYG +R S + +C ++ ++V+GK++LC+ P ++GA+ ++
Sbjct: 369 KKYPLVYGKSASSR-CDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASIV 428
Query: 433 MQSGTRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSS 492
++ D A + P +++S D + + T P A KS + APV+AS+SS
Sbjct: 429 -RNPYEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSS 488
Query: 493 RGPNSLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVA 552
RGPN L + ILKPD++ PG EIL A+ P P +E D+R Y +ISGTSMSC H+ GVA
Sbjct: 489 RGPNPLIHDILKPDITAPGSEILAAYSPYVPPSE-SDTRHVKYTVISGTSMSCPHVAGVA 548
Query: 553 VYVKTFNPTLSPAAIKSALMTTASPMNASL---HSQAEFAYGAGHVNPLKAVNPGLVYDA 612
Y+KTF+P SP+ I+SA+MTTA PMNAS + AEFAYGAGHV+P+ A++PGLVY+A
Sbjct: 549 AYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEA 608
Query: 613 DENEYVKFLCGQGYDTAMVRRITGDNSACTSGNIGRVW-DLNLPSFALSVAAGSKALNQY 672
++++++ FLCG Y +R I+GD+S+CT + +LN PS + V +G+K
Sbjct: 609 NKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQV-SGTKPFKVT 668
Query: 673 FTRTLTNVASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLV 732
F RT+TNV +TY+A + K L++ V P VLS + E+KSF +TV G + LV
Sbjct: 669 FRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAENLV 728
Query: 733 SACLVWSDGVHSVRSPITI 737
SA L+WSDGVH VRSPI +
Sbjct: 729 SAQLIWSDGVHFVRSPIVV 734
BLAST of CmoCh04G012250 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 595.1 bits (1533), Expect = 7.6e-170
Identity = 329/733 (44.88%), Postives = 457/733 (62.35%), Query Frame = 0
Query: 17 LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 76
L+ L +L+S S D+D ++YIVYMGS P+S H+ ++L+QV G +
Sbjct: 10 LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69
Query: 77 QSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 136
++ +Y RSFNGF LTE E IA EGVVSVF N+ LHTT SWDFMG + N
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129
Query: 137 DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIGARAY 196
R +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C NF+CN K+IGAR Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 197 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 256
RDT+GHGTHTAS AG V S + +G GT RGG P++RIA YK+
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249
Query: 257 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 316
C GCS +L++FDDAIADGVD I++S+G P + DPIAIG+FHAM KGILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309
Query: 317 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLV 376
AGN GPK +T + ++PW+ +VAA+T +R F+T+V LG+G+ G S+N F+ +YPLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369
Query: 377 YGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTR 436
YG + + C +N +V+GK+L+C + S+GAI +I +S
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRP 429
Query: 437 DNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSL 496
D A ++ LPA+ + +D ++ + +P A K+ + ++PVIASFSSRGPN++
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489
Query: 497 ANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTF 556
A ILKPD++ PGVEIL A+ P +E D+R Y++ SGTSM+C H+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549
Query: 557 NPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 616
P SP+ I+SA+MTTA P+ A + EFAYGAGHV+P+ A+NPGLVY+ D+ +++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609
Query: 617 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 676
FLCG Y + ++ I+GD C+ N +LN PS + ++ + F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669
Query: 677 ASSGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLVSACLVW 736
+ STY++ + A G L I V P VL F + E++SF++TV G+ S SA L+W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 728
BLAST of CmoCh04G012250 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 591.7 bits (1524), Expect = 8.4e-169
Identity = 327/731 (44.73%), Postives = 454/731 (62.11%), Query Frame = 0
Query: 17 LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 76
L+ L +L+S S D+D ++YIVYMGS P+S H+ ++L+QV G +
Sbjct: 10 LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69
Query: 77 QSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 136
++ +Y RSFNGF LTE E IA EGVVSVF N+ LHTT SWDFMG + N
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129
Query: 137 DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIGARAY 196
R +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C NF+CN K+IGAR Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 197 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 256
RDT+GHGTHTAS AG V S + +G GT RGG P++RIA YK+
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249
Query: 257 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 316
C GCS +L++FDDAIADGVD I++S+G P + DPIAIG+FHAM KGILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309
Query: 317 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLV 376
AGN GPK +T + ++PW+ +VAA+T +R F+T+V LG+G+ G S+N F+ +YPLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369
Query: 377 YGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTR 436
YG + + C +N +V+GK+L+C + S+GAI +I +S
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRP 429
Query: 437 DNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSL 496
D A ++ LPA+ + +D ++ + +P A K+ + ++PVIASFSSRGPN++
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489
Query: 497 ANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTF 556
A ILKPD++ PGVEIL A+ P +E D+R Y++ SGTSM+C H+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549
Query: 557 NPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVKFL 616
P SP+ I+SA+MTTA + EFAYGAGHV+P+ A+NPGLVY+ D+ +++ FL
Sbjct: 550 YPRWSPSMIQSAIMTTAKGRGI---ASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFL 609
Query: 617 CGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNVAS 676
CG Y + ++ I+GD C+ N +LN PS + ++ + F RTLTNV +
Sbjct: 610 CGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGT 669
Query: 677 SGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLVSACLVWSD 736
STY++ + A G L I V P VL F + E++SF++TV G+ S SA L+WSD
Sbjct: 670 PNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSD 723
BLAST of CmoCh04G012250 vs. TAIR 10
Match:
AT5G59090.3 (subtilase 4.12 )
HSP 1 Score: 591.3 bits (1523), Expect = 1.1e-168
Identity = 329/733 (44.88%), Postives = 457/733 (62.35%), Query Frame = 0
Query: 17 LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 76
L+ L +L+S S D+D ++YIVYMGS P+S H+ ++L+QV G +
Sbjct: 10 LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69
Query: 77 QSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 136
++ +Y RSFNGF LTE E IA EGVVSVF N+ LHTT SWDFMG + N
Sbjct: 70 GRLVRSYKRSFNGFAARLTESERTLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129
Query: 137 DRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-VSNFSCNRKIIGARAY 196
R +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C NF+CN K+IGAR Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189
Query: 197 HIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVYKI 256
RDT+GHGTHTAS AG V S + +G GT RGG P++RIA YK+
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249
Query: 257 CWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTSNS 316
C GCS +L++FDDAIADGVD I++S+G P + DPIAIG+FHAM KGILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309
Query: 317 AGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYPLV 376
AGN GPK +T + ++PW+ +VAA+T +R F+T+V LG+G+ G S+N F+ +YPLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369
Query: 377 YGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSGTR 436
YG + + C +N +V+GK+L+C + S+GAI +I +S
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRP 429
Query: 437 DNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPNSL 496
D A ++ LPA+ + +D ++ + +P A K+ + ++PVIASFSSRGPN++
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489
Query: 497 ANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVKTF 556
A ILKPD++ PGVEIL A+ P +E D+R Y++ SGTSM+C H+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGEPSE-DDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549
Query: 557 NPTLSPAAIKSALMTTASPMNASLH--SQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 616
P SP+ I+SA+MTTA P+ A + EFAYGAGHV+P+ A+NPGLVY+ D+ +++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609
Query: 617 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 676
FLCG Y + ++ I+GD C+ N +LN PS + ++ + F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669
Query: 677 ASSGSTYRATISAPKG--LRITVNPCVLSFNRIGERKSFNLTVGGT--VSRRLVSACLVW 736
+ STY++ + A G L I V P VL F + E++SF++TV G+ S SA L+W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 726
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 8.8e-288 | 68.26 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIG2 | 1.5e-170 | 44.22 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9STF7 | 2.8e-169 | 44.93 | Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... | [more] |
| Q8L7D2 | 1.1e-168 | 44.88 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Q9FIF8 | 6.9e-168 | 43.32 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1H1P2 | 0.0e+00 | 100.00 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1 | [more] |
| A0A6J1GZY5 | 0.0e+00 | 82.74 | cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1 | [more] |
| A0A4P2YW59 | 4.1e-304 | 72.25 | Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2... | [more] |
| K7NBW1 | 7.2e-293 | 70.08 | Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1 | [more] |
| A0A1S3CF86 | 3.8e-286 | 68.53 | LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1 | [more] |
