CmoCh04G011350 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G011350
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAlpha-galactosidase
LocationCmo_Chr04: 5789439 .. 5792616 (+)
RNA-Seq ExpressionCmoCh04G011350
SyntenyCmoCh04G011350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTACATTCATCCTCTCTTCCACATATATGATCGAAGTAATGATTTAGGGTTTACTTGGAATGATTTGGCTTCTACCATTTAGTTCAATCTTAGTATGCATGTAATGATTTGGCTTCTACCATTTAGTTCCTACTCTTCCGATGTTCTGGTGAAACATTGGTTTATGTCTATAATGTAATGATTTTCTAGGACACAAATGATTTTTTATTATTATTAAGAAGCTTAATCGTTACTATTTTAGTTATAAATTGTACAATTAAGAATTGAACCATTGACTTTTTAGATAGTAATTAATGTTTTATCTACTATGGTGTGTGGTAGGGGCGTTGATGATCCGGCATTATGGGCTCCCAGAGTTGGAAATAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGGTTAGGTTTAAATCTAACCCTAAAATCTTCCCATTATTGCCATAAATAACCCCTAAAATGTTGCATTAACTTCAAAAAAATTGTTTTTTGTTTTGGTTGAAGCATGACTAGAATTGCAGATATCAACGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGGTATGAGATTGATTGTGTTTGAAACCCATTCAACTTCATCCATTTGTTCTCTAAGTTGATCTCTAATTCCCATCTTACCATGTGTGCGTGGACAGATCCAGATATGTTGGAAGTTGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGTTTCAATTTTACTGTAACGATAGAATTTGAAAATTTTCTTAGGTATACGGCCAACAGATCAGATCAGAAGCTTGAATACTATTTGAATATTAACAAATAAGGAACTCAGATAAAAATAGCATAATAAGATTTACTTTCTAAAAACAAAAAGTCAAATGGTTATCAAACATGCACGTTTTGAGTTCATCCAAATGTGCATGCAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAACATGTCTCCACAAACTTTGGAAATTCTAAGCAATAAGGAAGTCATTGATGTAAACCAAGGTGATCAACATTTATTTATACTATCTTTTCTTATCTTATACTATCTTATACCCTTTTAAACAAGAACACTTAATATTTGCTCACATATCAAAAGGGAAACTTAATTTATGAACGCAGATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTACTCTTTTTAACTTTTGCTTCATCTTACATTGTATTAAGCATAACAAAGGTATGAATTTAAACTTCAAACTCGTCTTATTTAGTTTACAGTTTTTATTAGATCGTTAAACTTTCTGGTGTTTCTTAGGTGTGGGTTTAGGTGTTTGGTGAACGAGTCAATGAGTGAGGCATGCAAACCAGCAAAAAAAAAAAAAAAAAAAAACCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAACCTACACAAGAAACACCAAGAAAAACCTAAACCCATTTAAAACCAATTTACCCCACCGTCCTTATAAATCTAGTCAACGTGCATGAGTGTTACACTAACAGAATTAACGACAGATATATTTTATTACACACAAAATTAAAAATTTCAAACGCTTGACAAAAAACTTATAGTTTAAGGACTATAAGTACATTTATATCGCCTACACTTTGCTAGATTAATATTTTATTTTGGCACATAAGAAGAGAACAGCATGACTTACAATGGCTCCATGAGAAGCAAAGTAAAAGGAAAGAGTTGTGACTTACAGGTGTGGGCAGGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGGTCAGGATCCTAGCTCATCTAAACTAATTTACTCAGTATGCATGAACCGATATTCTCATCTTAGTTAACGACTTCTATGAAGCATCGACACTTTAGTTTAAATGACATGACAATGTGCCTATGTCTTGTGTCTGTGTACGTGCTTCAGTCTTTAGTCTATCTTGGTGCAATGCAGTAGAAATATTAGAGTTGTTTAATCTAACTTGGTCGTCTCATCACCAATACTTGCAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAGACAATGCTCAGAGACGGGAAGGCCAAGAGATTTAGGAAGTTGTGAAAGAGCTAGATTATGTCATTACTTAAATAAAAAGGGTATCTAGTTTCTCGATCTCTATTCCTTTATGTAAAAGGTAATCAAAAGTCGAGAGATTCGTATCCAACATGAGTCCTTTAAATCAACTAGTCTCTCTTTATAAGTGACTTCTGAGTGATAATAAAATATGTTGTGAAGTATTATTGAAGGGTGTTCCTATCTAGAACCTATCAGTTATACTATTGAATCTCTTTCTTAGTATCAACAGAAAATTTATACTATTAAGCTTTCGT

mRNA sequence

ATGTTCAATCTTAGTATGCATGTAATGATTTGGCTTCTACCATTTAGTTCCTACTCTTCCGATGTTCTGGTGAAACATTGGTTTATGTCTATAATGGGCGTTGATGATCCGGCATTATGGGCTCCCAGAGTTGGAAATAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGAATTGCAGATATCAACGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGATCCAGATATGTTGGAAGTTGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAACATGTCTCCACAAACTTTGGAAATTCTAAGCAATAAGGAAGTCATTGATATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTGTGGGTTTAGGTGTTTGGTGAACGAGTCAATGAGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAGACAATGCTCAGAGACGGGAAGGCCAAGAGATTTAGGAAGTTGTGAAAGAGCTAGATTATGTCATTACTTAAATAAAAAGGGTATCTAGTTTCTCGATCTCTATTCCTTTATGTAAAAGGTAATCAAAAGTCGAGAGATTCGTATCCAACATGAGTCCTTTAAATCAACTAGTCTCTCTTTATAAGTGACTTCTGAGTGATAATAAAATATGTTGTGAAGTATTATTGAAGGGTGTTCCTATCTAGAACCTATCAGTTATACTATTGAATCTCTTTCTTAGTATCAACAGAAAATTTATACTATTAAGCTTTCGT

Coding sequence (CDS)

ATGTTCAATCTTAGTATGCATGTAATGATTTGGCTTCTACCATTTAGTTCCTACTCTTCCGATGTTCTGGTGAAACATTGGTTTATGTCTATAATGGGCGTTGATGATCCGGCATTATGGGCTCCCAGAGTTGGAAATAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGAATTGCAGATATCAACGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGATCCAGATATGTTGGAAGTTGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAACATGTCTCCACAAACTTTGGAAATTCTAAGCAATAAGGAAGTCATTGATATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTGTGGGTTTAGGTGTTTGGTGAACGAGTCAATGAGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAG

Protein sequence

MFNLSMHVMIWLLPFSSYSSDVLVKHWFMSIMGVDDPALWAPRVGNSWRTTIDISDSWERIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Homology
BLAST of CmoCh04G011350 vs. ExPASy Swiss-Prot
Match: Q8VXZ7 (Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 6.3e-65
Identity = 122/221 (55.20%), Postives = 153/221 (69.23%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSW---ERIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWA  VGNSWRTT DI+D+W     IAD+N+KWA YAGPGGWNDPDMLE+GNGG
Sbjct: 228 GVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGG 287

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTY+EYR HFSIWALMKAPLLIGCDVRNM+ +TLEILSNKE+I +    LG +  K++  
Sbjct: 288 MTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQAN 347

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            +            N+   + S      R+ + LWNRCS  + I+ SW  +G+   I VS
Sbjct: 348 GE------------NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVS 407

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 248
           VRDLW+HK +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 408 VRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 436

BLAST of CmoCh04G011350 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-54
Identity = 104/210 (49.52%), Postives = 135/210 (64.29%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           G +DP +WA  +GNSWRTT DI D+W     IAD NDKWA YAGPGGWNDPDMLEVGNGG
Sbjct: 212 GWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDMLEVGNGG 271

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MT +EYRSHFSIWAL KAPLL+GCD+R M   T E++SN EVI +     ++KL    K 
Sbjct: 272 MTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAV----NQDKLGVQGKK 331

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           V             + + +    + ++A++LWNR S+ + ++ SW ++G+     V  RD
Sbjct: 332 V--------KSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTTVDARD 391

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
           LWEH    S+      AE+DSH CKMY++T
Sbjct: 392 LWEHS-TQSLVSGEISAEIDSHACKMYVLT 408

BLAST of CmoCh04G011350 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 4.2e-53
Identity = 107/213 (50.23%), Postives = 135/213 (63.38%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWERI---ADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           G +DPA WA  VGNSWRTT DI DSW  +   AD+NDKWA YAGPGGWNDPDMLEVGNGG
Sbjct: 180 GEEDPATWAKEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGG 239

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MT  EYRSHFSIWAL KAPLLIGCD+R+M   T ++LSN EVI +    LG +  K+K  
Sbjct: 240 MTTTEYRSHFSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTY 299

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
             + V+               +      R+A+ LWNR S+ + I+  W ++G+    +V+
Sbjct: 300 GDLEVW---------------AGPLSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVN 359

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
            RDLW H    S+ +    A VD+HD KMY++T
Sbjct: 360 ARDLWAHSTEKSV-KGQISAAVDAHDSKMYVLT 376

BLAST of CmoCh04G011350 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.4e-51
Identity = 104/210 (49.52%), Postives = 133/210 (63.33%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWER---IADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           G +DPA WA  +GNSWRTT DI D+W+    IAD ND+WA YA PG WNDPDMLEVGNGG
Sbjct: 196 GQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGG 255

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MT +EY SHFSIWAL KAPLLIGCD+R+M   T E+LSNKEVI +     ++KL    K 
Sbjct: 256 MTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAV----NQDKLGIQGKK 315

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           V             + + +    + R+A++LWNR S ++ I+  W E+G+    IV+ RD
Sbjct: 316 V--------KKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARD 375

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
           LWEH     + +    A V+ H CKMY +T
Sbjct: 376 LWEHSTYSCVKK-QLSALVEPHACKMYTLT 392

BLAST of CmoCh04G011350 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.8e-51
Identity = 108/220 (49.09%), Postives = 137/220 (62.27%), Query Frame = 0

Query: 25  KHWFMSI--MGVDDPALWAPRVGNSWRTTIDISDSWERI---ADINDKWAEYAGPGGWND 84
           K+ F S+   G ++PA WA R+GNSWRTT DI+D+W  +   AD ND+WA YAGPGGWND
Sbjct: 210 KNIFFSLCEWGKENPATWAGRMGNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWND 269

Query: 85  PDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSR 144
           PDMLEVGNGGM+  EYRSHFSIWAL KAPLLIGCDVR+MS QT  ILSN EVI +     
Sbjct: 270 PDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIGCDVRSMSQQTKNILSNSEVIAV----N 329

Query: 145 EEKLKFLAKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGV 204
           ++ L    K V          +  + + +      R A+VLWNR S  + I+  W  +G+
Sbjct: 330 QDSLGVQGKKV--------QSDNGLEVWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGL 389

Query: 205 IFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
              + V+ RDLW H      ++    A V  HDCKMY++T
Sbjct: 390 AGSVAVTARDLWAHS--SFAAQGQISASVAPHDCKMYVLT 415

BLAST of CmoCh04G011350 vs. ExPASy TrEMBL
Match: A0A6J1FS41 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.7e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 262 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 321

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 322 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 381

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 382 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 441

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 442 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 475

BLAST of CmoCh04G011350 vs. ExPASy TrEMBL
Match: A0A6J1FXE1 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.7e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 265 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 324

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 325 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 384

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 385 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 444

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 445 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 478

BLAST of CmoCh04G011350 vs. ExPASy TrEMBL
Match: A0A6J1FQU0 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.7e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 264 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 323

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 324 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 383

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 384 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 443

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 444 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 477

BLAST of CmoCh04G011350 vs. ExPASy TrEMBL
Match: A0A6J1JG86 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111484225 PE=3 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.4e-99
Identity = 186/219 (84.93%), Postives = 192/219 (87.67%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 251 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 310

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+     ++ L    + 
Sbjct: 311 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDV----NQDSLGLQGRK 370

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           +       CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFD+IVSVRD
Sbjct: 371 INVSGKDGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDVIVSVRD 430

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 431 LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 464

BLAST of CmoCh04G011350 vs. ExPASy TrEMBL
Match: A0A6J1CZ60 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015929 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 5.9e-82
Identity = 154/215 (71.63%), Postives = 173/215 (80.47%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAP++GNSWRTTIDISDSW+    IADINDKWA YAGPGGWNDPDMLEVGNGG
Sbjct: 246 GVDDPALWAPKIGNSWRTTIDISDSWDSMTSIADINDKWAAYAGPGGWNDPDMLEVGNGG 305

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MTY EYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNK+VI++     +++L    + 
Sbjct: 306 MTYNEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKQVIEV----NQDRLGIQGRK 365

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           +       CL   +  L      E+RLA+VLWNRCS ASMIS++WLELG I D I SVRD
Sbjct: 366 IKVSGKDGCLQVWAGPLSGQYGSEMRLAVVLWNRCSYASMISITWLELGFILDYIFSVRD 425

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLF 245
           LWEHKF+  I RDSFGAEVDSHDCKMYI+T+IPLF
Sbjct: 426 LWEHKFV-LIGRDSFGAEVDSHDCKMYIITYIPLF 455

BLAST of CmoCh04G011350 vs. NCBI nr
Match: XP_022943110.1 (alpha-galactosidase 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 7.6e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 265 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 324

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 325 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 384

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 385 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 444

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 445 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 478

BLAST of CmoCh04G011350 vs. NCBI nr
Match: XP_022943111.1 (alpha-galactosidase 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 7.6e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 264 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 323

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 324 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 383

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 384 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 443

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 444 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 477

BLAST of CmoCh04G011350 vs. NCBI nr
Match: XP_022943112.1 (alpha-galactosidase 3-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 7.6e-100
Identity = 191/222 (86.04%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 262 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 321

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 322 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 381

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS
Sbjct: 382 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 441

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 442 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 475

BLAST of CmoCh04G011350 vs. NCBI nr
Match: XP_023529356.1 (alpha-galactosidase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.0 bits (959), Expect = 9.9e-100
Identity = 190/222 (85.59%), Postives = 194/222 (87.39%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 251 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 310

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+    LG +  K+K  
Sbjct: 311 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDVNQDSLGLQGRKIKVS 370

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            K         CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFD+IVS
Sbjct: 371 GK-------DGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDVIVS 430

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 431 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 464

BLAST of CmoCh04G011350 vs. NCBI nr
Match: XP_022986509.1 (alpha-galactosidase 3-like [Cucurbita maxima])

HSP 1 Score: 371.7 bits (953), Expect = 4.9e-99
Identity = 186/219 (84.93%), Postives = 192/219 (87.67%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWE---RIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWAPRVGNSWRTTIDISDSWE   RIADINDKWAEYAGPGGWNDPDMLEVGNGG
Sbjct: 251 GVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGG 310

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVID+     ++ L    + 
Sbjct: 311 MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDV----NQDSLGLQGRK 370

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           +       CL      LLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFD+IVSVRD
Sbjct: 371 INVSGKDGCL-QVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDVIVSVRD 430

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 249
           LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 431 LWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 464

BLAST of CmoCh04G011350 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 248.8 bits (634), Expect = 4.5e-66
Identity = 122/221 (55.20%), Postives = 153/221 (69.23%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSW---ERIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWA  VGNSWRTT DI+D+W     IAD+N+KWA YAGPGGWNDPDMLE+GNGG
Sbjct: 204 GVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGG 263

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTY+EYR HFSIWALMKAPLLIGCDVRNM+ +TLEILSNKE+I +    LG +  K++  
Sbjct: 264 MTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQAN 323

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            +            N+   + S      R+ + LWNRCS  + I+ SW  +G+   I VS
Sbjct: 324 GE------------NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVS 383

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 248
           VRDLW+HK +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 384 VRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 412

BLAST of CmoCh04G011350 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 248.8 bits (634), Expect = 4.5e-66
Identity = 122/221 (55.20%), Postives = 153/221 (69.23%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSW---ERIADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           GVDDPALWA  VGNSWRTT DI+D+W     IAD+N+KWA YAGPGGWNDPDMLE+GNGG
Sbjct: 228 GVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGG 287

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDI---VLGSREEKLKFL 152
           MTY+EYR HFSIWALMKAPLLIGCDVRNM+ +TLEILSNKE+I +    LG +  K++  
Sbjct: 288 MTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQAN 347

Query: 153 AKMVVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVS 212
            +            N+   + S      R+ + LWNRCS  + I+ SW  +G+   I VS
Sbjct: 348 GE------------NDCQQVWSGPLSGDRMVVALWNRCSEPATITASWDMIGLESTISVS 407

Query: 213 VRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 248
           VRDLW+HK +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 408 VRDLWQHKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 436

BLAST of CmoCh04G011350 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 204.5 bits (519), Expect = 9.7e-53
Identity = 104/210 (49.52%), Postives = 133/210 (63.33%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWER---IADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           G +DPA WA  +GNSWRTT DI D+W+    IAD ND+WA YA PG WNDPDMLEVGNGG
Sbjct: 196 GQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGG 255

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MT +EY SHFSIWAL KAPLLIGCD+R+M   T E+LSNKEVI +     ++KL    K 
Sbjct: 256 MTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAV----NQDKLGIQGKK 315

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           V             + + +    + R+A++LWNR S ++ I+  W E+G+    IV+ RD
Sbjct: 316 V--------KKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARD 375

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
           LWEH     + +    A V+ H CKMY +T
Sbjct: 376 LWEHSTYSCVKK-QLSALVEPHACKMYTLT 392

BLAST of CmoCh04G011350 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 204.5 bits (519), Expect = 9.7e-53
Identity = 104/210 (49.52%), Postives = 133/210 (63.33%), Query Frame = 0

Query: 33  GVDDPALWAPRVGNSWRTTIDISDSWER---IADINDKWAEYAGPGGWNDPDMLEVGNGG 92
           G +DPA WA  +GNSWRTT DI D+W+    IAD ND+WA YA PG WNDPDMLEVGNGG
Sbjct: 170 GQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGG 229

Query: 93  MTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKM 152
           MT +EY SHFSIWAL KAPLLIGCD+R+M   T E+LSNKEVI +     ++KL    K 
Sbjct: 230 MTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAV----NQDKLGIQGKK 289

Query: 153 VVFRCGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRD 212
           V             + + +    + R+A++LWNR S ++ I+  W E+G+    IV+ RD
Sbjct: 290 V--------KKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIVNARD 349

Query: 213 LWEHKFLDSISRDSFGAEVDSHDCKMYIMT 240
           LWEH     + +    A V+ H CKMY +T
Sbjct: 350 LWEHSTYSCVKK-QLSALVEPHACKMYTLT 366

BLAST of CmoCh04G011350 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-46
Identity = 96/205 (46.83%), Postives = 130/205 (63.41%), Query Frame = 0

Query: 37  PALWAPRVGNSWRTTIDISDSW---ERIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQ 96
           PALW   VGNSWRTT DI D+W     IAD+N+ +AE+A PGGWNDPDMLEVGNGGMT  
Sbjct: 214 PALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKD 273

Query: 97  EYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKEVIDIVLGSREEKLKFLAKMVVFR 156
           EY  HFSIWA+ KAPLL+GCD+RNM+ +T+EI++NKEVI I     ++     AK V   
Sbjct: 274 EYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANKEVIAI----NQDPHGVQAKKV--- 333

Query: 157 CGFRCLVNESMSLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEH 216
                 +   + + +      R+A++L NR  + + I+  W ++ +  + IV  RDLWEH
Sbjct: 334 -----RMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEH 393

Query: 217 KFLDSISRDSFGAEVDSHDCKMYIM 239
           + L      +  A VDSH CK+Y++
Sbjct: 394 QTLKQKFVGNLTATVDSHACKLYVL 406

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VXZ76.3e-6555.20Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1[more]
P147491.7e-5449.52Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q426564.2e-5350.23Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
Q8RX861.4e-5149.52Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
Q9FXT41.8e-5149.09Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1FS413.7e-10086.04Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1[more]
A0A6J1FXE13.7e-10086.04Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1[more]
A0A6J1FQU03.7e-10086.04Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1[more]
A0A6J1JG862.4e-9984.93Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111484225 PE=3 SV=1[more]
A0A6J1CZ605.9e-8271.63Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015929 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022943110.17.6e-10086.04alpha-galactosidase 3-like isoform X1 [Cucurbita moschata][more]
XP_022943111.17.6e-10086.04alpha-galactosidase 3-like isoform X2 [Cucurbita moschata][more]
XP_022943112.17.6e-10086.04alpha-galactosidase 3-like isoform X3 [Cucurbita moschata][more]
XP_023529356.19.9e-10085.59alpha-galactosidase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022986509.14.9e-9984.93alpha-galactosidase 3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G56310.24.5e-6655.20Melibiase family protein [more]
AT3G56310.14.5e-6655.20Melibiase family protein [more]
AT5G08370.19.7e-5349.52alpha-galactosidase 2 [more]
AT5G08370.29.7e-5349.52alpha-galactosidase 2 [more]
AT5G08380.11.4e-4646.83alpha-galactosidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 72..91
score: 73.75
coord: 93..114
score: 55.68
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 45..134
e-value: 1.5E-23
score: 83.4
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 32..239
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 175..237
e-value: 2.0E-8
score: 34.2
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 23..135
e-value: 2.0E-44
score: 154.3
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 154..240
e-value: 1.6E-14
score: 55.6
NoneNo IPR availablePANTHERPTHR11452:SF75ALPHA-GALACTOSIDASE 3coord: 32..239
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 176..239
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 28..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G011350.1CmoCh04G011350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds