CmoCh04G011070 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G011070
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationCmo_Chr04: 5643083 .. 5644895 (+)
RNA-Seq ExpressionCmoCh04G011070
SyntenyCmoCh04G011070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAACACCATCTTTATCCAATATAAATACAACCCCTTCTCTTTAATTTCCTCTAATTTCTTTCTATGATGATGTTTTCTTGGCGACGCCGGAGGGCTACCCGGAGGATGGCTAGAGGTGGCGGCGGTGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGGCTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCAGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAAAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGTTCGTAAACTACTTCATAGCTAACATCCCATCTCGGTTGGGGAGGAGAACGAAACACCCTTTATTGCAAGGGTGTTTAAACCTTTCCTTGACCGACCCATTTTAAAATTATCGAGAAAAAGTTCAAATAGGACAATATCTGCCAGGGGTGACCTTCGACTCTTACAAATGAGCGTCCACTTTGGTTGAGGAGTATAACGAAACACCTTTTGTTACAAGGGTCGGTAAACCTCTCCCTAACCGACCCATTTTAAAATTATTAAGGAAAAGCTCAAATAAGACTCTTACAAATTAGCTACGAGATTCAAAGGTGGAACCTTTTTATCATTATTTTTGTTTATATGTTAACAGGAAATGTATTATCCTCCGCATTCGAAGCGTTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAGCCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGTTTCACAAAGGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCGTTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGTGACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAAGAAGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGATTCTAAATTTACTTACATTACTCATGTTTTTTTGTTTGTTGTACAAAATAA

mRNA sequence

TAAACACCATCTTTATCCAATATAAATACAACCCCTTCTCTTTAATTTCCTCTAATTTCTTTCTATGATGATGTTTTCTTGGCGACGCCGGAGGGCTACCCGGAGGATGGCTAGAGGTGGCGGCGGTGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGGCTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCAGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAAAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGAAATGTATTATCCTCCGCATTCGAAGCGTTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAGCCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGTTTCACAAAGGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCGTTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGTGACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAAGAAGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGATTCTAAATTTACTTACATTACTCATGTTTTTTTGTTTGTTGTACAAAATAA

Coding sequence (CDS)

ATGATGATGTTTTCTTGGCGACGCCGGAGGGCTACCCGGAGGATGGCTAGAGGTGGCGGCGGTGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGGCTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCAGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAAAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGAAATGTATTATCCTCCGCATTCGAAGCGTTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAGCCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGTTTCACAAAGGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCGTTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGTGACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAAGAAGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTTTTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGA

Protein sequence

MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDFKDLKRTF
Homology
BLAST of CmoCh04G011070 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 4.9e-100
Identity = 203/423 (47.99%), Postives = 271/423 (64.07%), Query Frame = 0

Query: 27  AELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIP 86
           +E+TIP  F CPIS+DLMKDPV +STGITYDR +IETWI  GN +CP TN  L T D IP
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIP 86

Query: 87  NHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEK 146
           NH IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A RR D E C  ++EK
Sbjct: 87  NHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEK 146

Query: 147 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 206
           IK L  ESE+N+KC+  N  G VL   F+ FS     EK   +L EILS +  +FP+  E
Sbjct: 147 IKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLE 206

Query: 207 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKL 266
            I  L S  S + +   ++  D S R+++  ++KEI+S    +V       GV E L+KL
Sbjct: 207 GISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKL 266

Query: 267 IKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALG 326
           I++ + SSS K+SL   Y  +            FL++GLV + +E +VDAE SVCE+AL 
Sbjct: 267 IRDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALA 326

Query: 327 VFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDED 386
           V D ICET+ GREE   +AL MP++VKKI +VS+L T  S+S++ KL K  +    ED  
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED-- 386

Query: 387 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSL-DFKDLK 446
                 A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +K
Sbjct: 387 ------AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 447 RTF 449
           + F
Sbjct: 447 KPF 435

BLAST of CmoCh04G011070 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.8e-84
Identity = 183/425 (43.06%), Postives = 261/425 (61.41%), Query Frame = 0

Query: 28  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 87
           E+TIPS F CPIS +LMKDPV +++GITYDR NIE W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 88  HNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEKI 147
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 148 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 207
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 208 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 267
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 268 KEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALGV 327
                 S+   SL   +H I   +NQE  ++ FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQETVSR-FLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 328 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDEDG 387
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K          DG
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--------SGDG 389

Query: 388 GVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFKD 447
               EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK 
Sbjct: 390 SEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKH 431

Query: 448 LKRTF 449
           +K+ F
Sbjct: 450 VKKPF 431

BLAST of CmoCh04G011070 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.5e-52
Identity = 138/419 (32.94%), Postives = 217/419 (51.79%), Query Frame = 0

Query: 29  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIE-GGNFSCPFTNQPLHTIDSIPN 88
           + IP HF CPISL+LM+DPVT+ TG TYDRA+IE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 89  HNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAV-RREDCEGCKSLVEK 148
           H +R++IQ WCV NR++G+ERIPTP+ PA P  VR +L + +A            + + +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 149 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 208
           ++   ++S++N+  I A+    +L      FS+        E++ E L+ + M+   +  
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITEPN 191

Query: 209 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEI---QGVLEGL 268
               + S+      +  +  +     R +   L EI+S+  +     G I   + V EG+
Sbjct: 192 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGV 251

Query: 269 MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL-VDAERSVCE 328
           + L++ PI   S + +L I    + +  + +      +  G   +L++ L  D +R   E
Sbjct: 252 LDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 311

Query: 329 RALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEE 388
           RAL   + +C T +G      HALT+P++VK ILRVSD  TE +   +  L   E R  E
Sbjct: 312 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWRE 371

Query: 389 EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDF 442
           E    GV V+         LLL++Q  C+E  K+KA +LLKLL +   +     +S DF
Sbjct: 372 EAAGAGVVVQ---------LLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 412

BLAST of CmoCh04G011070 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 6.1e-50
Identity = 134/404 (33.17%), Postives = 208/404 (51.49%), Query Frame = 0

Query: 29  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPNH 88
           + IP HF CPISLDLM DPVT+STG TYDR +I++WI  GN +CP T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 89  NIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAV-RREDCEGCKSLVEKI 148
            +R++IQ WCV NR++G+ERIPTP+ PA P+ VR +L + +A            + + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 149 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 208
           + L ++SE+N+  I  +    +L      F+  E      E++ E L+ + ++  + E  
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLH-MTETE 191

Query: 209 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQG---VLEGLM 268
            + + S+ S    +  +  +     R +   L E++ +  + +     I G   + EG++
Sbjct: 192 CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 269 KLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL-VDAERSVCER 328
            L+K PI   S + +L I   AI +    +      +  G   +L++ L  D +R   ER
Sbjct: 252 DLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 311

Query: 329 ALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEE 388
            L   + +C   +G      HALT+P++VK ILRVSD  TE +   +  L   E R    
Sbjct: 312 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEER---- 371

Query: 389 DEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 428
                 + EA   G   +LLLL+Q  C+E  K KA  LLKLL +
Sbjct: 372 -----CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 400

BLAST of CmoCh04G011070 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.7e-48
Identity = 130/434 (29.95%), Postives = 227/434 (52.30%), Query Frame = 0

Query: 28  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWI-EGGNFSCPFTNQPLHTIDSIP 87
           E+ IPS FLCPISLD+MKDPV +STGITYDR +IE W+  G   SCP T Q +   D  P
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 88  NHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAA-VRREDCEGCKSLVE 147
           NH +R++IQ+WC  N ++GIERIPTP+ P    ++ +++ + +++ + +  C      ++
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKC------LK 123

Query: 148 KIKILVQESERNKKCI----IANGTGNVLSSAFEAFSKP-------------------ER 207
           +++ +V E+  NK+C+    +     N++S++ + ++ P                    R
Sbjct: 124 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 183

Query: 208 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFV-----RGEDKSRRRSSIL 267
           F+    +++E LS +  +    E A+K L +      LV  +     RG  +SR  +++L
Sbjct: 184 FDSSRSLMDEALSVLYHL-DTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALL 243

Query: 268 VLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT 327
           + K +  + P ++  L   + +   +++++ + I   + ++++ I         N+    
Sbjct: 244 LKKLLEVADPMQIILLE--RELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAV 303

Query: 328 KAFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILR 387
           +      ++ LL++    +ER   E A+ V D +C+  +GR E   H   + V+ KKILR
Sbjct: 304 EGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILR 363

Query: 388 VSDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEK 432
           VS + +E +V ++  + +  +          +  E LQ+G   KL L+LQV C   TKEK
Sbjct: 364 VSQITSERAVRVLLSVGRFCATP-------SLLQEMLQLGVVAKLCLVLQVSCGNKTKEK 421

BLAST of CmoCh04G011070 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.1e-251
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60
           MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120
           IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180
           VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP
Sbjct: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240
           ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300
           EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300

Query: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 449
           LLKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 448

BLAST of CmoCh04G011070 vs. ExPASy TrEMBL
Match: A0A6J1K7Y6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 PE=4 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.0e-242
Identity = 434/448 (96.88%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60
           MMMFSWRRRRATRRMAR  GGGGGSM ELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN
Sbjct: 1   MMMFSWRRRRATRRMAR--GGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120
           IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180
           VREILDKIAAA  REDCE CKS+VEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP
Sbjct: 121 VREILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240
           ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300
           EIISSYPEKVSELGEIQGVLEG+MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300

Query: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLV LLLETL+DAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           L TELSVSIVWKLVKHESRE+E++EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 449
           LLKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 446

BLAST of CmoCh04G011070 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.1e-209
Identity = 379/450 (84.22%), Postives = 406/450 (90.22%), Query Frame = 0

Query: 2   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 61
           M+ SWRRRRATRRMAR   GG     ELTIP+HF CPISLDLMKDPVT+STGITYDRA+I
Sbjct: 1   MISSWRRRRATRRMAR---GGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASI 60

Query: 62  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQV 121
           ETWIEGGNF+CPFTNQPL TIDSIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QV
Sbjct: 61  ETWIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQV 120

Query: 122 REILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 181
           REIL+K+AAA RR DC+GCKS+VEKIK L +ESERNKKCIIANGTGN LSS FEAFS PE
Sbjct: 121 REILEKMAAAARRGDCDGCKSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPE 180

Query: 182 RFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 241
            FEKR+EILEEILS M + FPLQEE+IKHLKSE S+Q+LVWF++G D S RR+S+LVLKE
Sbjct: 181 TFEKRIEILEEILSAMTVAFPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKE 240

Query: 242 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIA---SSSNQEGFTK 301
           IISSYPEKV ELGEIQG LEGL+KLIK+PICSSS+KASLFITYH IA   SSSN+E FTK
Sbjct: 241 IISSYPEKVDELGEIQGALEGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTK 300

Query: 302 AFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRV 361
             LQMGLV LLLETLVDAERSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRV
Sbjct: 301 ELLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRV 360

Query: 362 SDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 421
           SDL TELSV IVWKLVKHES+EE   EDGG+KVEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 SDLATELSVCIVWKLVKHESKEE---EDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 422 TELLKLLNNLHRERLECIDSLDFKDLKRTF 449
           TELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 444

BLAST of CmoCh04G011070 vs. ExPASy TrEMBL
Match: A0A1S3CPH4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.4e-205
Identity = 374/450 (83.11%), Postives = 402/450 (89.33%), Query Frame = 0

Query: 2   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 61
           M+ SWRRRRAT RM R   G  G   ELTIP+HF CPISLDLMKDPVT+STGITYDRA+I
Sbjct: 1   MISSWRRRRATPRMPR---GSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASI 60

Query: 62  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQV 121
           ETWIEGGNF+CPFTNQPL TIDSIPNHNIRKMIQ WCVENRA+GIERIPTPRVPASP QV
Sbjct: 61  ETWIEGGNFTCPFTNQPLQTIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQV 120

Query: 122 REILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 181
           R+IL+K+ AA RR D EGCKS+VEKIK L +ESERNKKCIIANGTGN  SS FEAFS PE
Sbjct: 121 RDILEKMTAAARRGDFEGCKSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPE 180

Query: 182 RFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 241
            FEKR+EILEEILS M M+FPLQEE+IKHLKSE S+Q+L WF++G D S RR+S+LVLKE
Sbjct: 181 TFEKRIEILEEILSAMTMVFPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKE 240

Query: 242 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIA---SSSNQEGFTK 301
           IISSYPEKV ELG IQG LEGL+KLIK+PICSSSKKASLFITYH IA   SSSNQ  FTK
Sbjct: 241 IISSYPEKVDELGVIQGALEGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTK 300

Query: 302 AFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRV 361
           A LQMGLV LLLETLVDAERSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRV
Sbjct: 301 ALLQMGLVSLLLETLVDAERSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRV 360

Query: 362 SDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 421
           SDL TELSVSIVWKLVK+ES+EE+  EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 SDLATELSVSIVWKLVKNESKEEK--EDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 422 TELLKLLNNLHRERLECIDSLDFKDLKRTF 449
           TELLKLLNNLHR+RLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRDRLECIDSLDFKDLKRTF 445

BLAST of CmoCh04G011070 vs. ExPASy TrEMBL
Match: A0A6J1K8U9 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 PE=4 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.1e-201
Identity = 362/447 (80.98%), Postives = 401/447 (89.71%), Query Frame = 0

Query: 2   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 61
           M+ SWRRRRATRR AR   G GG M ELTIPSHF CPISLD+MKDPVT+STGITYDRA+I
Sbjct: 1   MISSWRRRRATRRTAR---GTGGCMRELTIPSHFRCPISLDMMKDPVTLSTGITYDRASI 60

Query: 62  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQV 121
           ETWIEGGNFSCP TNQPL T+DSIPNHNIRKMIQ WCVEN+A+G+ERIPTPRVPASPVQ+
Sbjct: 61  ETWIEGGNFSCPITNQPLQTLDSIPNHNIRKMIQDWCVENKAYGVERIPTPRVPASPVQI 120

Query: 122 REILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 181
           R+ILDKI AA RR D +GCKS VEKIK+L +ESERNKKCI+ NG  +V+SSAF+A SK E
Sbjct: 121 RDILDKITAATRRGDGDGCKSSVEKIKMLAKESERNKKCIMGNGASHVISSAFQALSKQE 180

Query: 182 RFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 241
             EK +EILEE++S MA++FPLQEE+IK+LKSEAS+QAL  F+RG D SRRR+SIL+LKE
Sbjct: 181 PLEKSIEILEEMISGMAIMFPLQEESIKNLKSEASLQALALFMRGGDLSRRRNSILILKE 240

Query: 242 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFL 301
           IISSYP+K ++LGEIQG LEGL+KLIKEPICSSSKKASLFITYH IASSSN + FTKA L
Sbjct: 241 IISSYPQKATKLGEIQGALEGLVKLIKEPICSSSKKASLFITYHIIASSSNPQRFTKALL 300

Query: 302 QMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMG V LLLETLVDAERS+CERALGVFDGICE+K+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGFVSLLLETLVDAERSICERALGVFDGICESKEGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 VTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 421
            TELSVSIVWKLVKHES    E+EDG VKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL
Sbjct: 361 ATELSVSIVWKLVKHES----EEEDGRVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 420

Query: 422 LKLLNNLHRERLECIDSLDFKDLKRTF 449
           LKLLNNLHR+RLECIDSLDFKD+KR F
Sbjct: 421 LKLLNNLHRDRLECIDSLDFKDIKRPF 440

BLAST of CmoCh04G011070 vs. NCBI nr
Match: XP_022942676.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 8.5e-251
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60
           MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120
           IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180
           VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP
Sbjct: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240
           ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300
           EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300

Query: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 449
           LLKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 448

BLAST of CmoCh04G011070 vs. NCBI nr
Match: KAG6600854.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 859.8 bits (2220), Expect = 1.1e-245
Identity = 444/450 (98.67%), Postives = 444/450 (98.67%), Query Frame = 0

Query: 1   MMMFSWRRRRATRRMAR-GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRA 60
           MMMFSWRRRRATRRMAR GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRA
Sbjct: 1   MMMFSWRRRRATRRMARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRA 60

Query: 61  NIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPV 120
           NIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPV
Sbjct: 61  NIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPV 120

Query: 121 QVREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSK 180
           QVREILDKIAAAV REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSK
Sbjct: 121 QVREILDKIAAAVGREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSK 180

Query: 181 PERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVL 240
           PERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVL
Sbjct: 181 PERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVL 240

Query: 241 KEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKA 300
           KEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT A
Sbjct: 241 KEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNA 300

Query: 301 FLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVS 360
           FLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVS
Sbjct: 301 FLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVS 360

Query: 361 DLVTELSVSIVWKLVKHESREEE-EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420
           DL TELSVSIVWKLVKHESREEE EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA
Sbjct: 361 DLATELSVSIVWKLVKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKA 420

Query: 421 TELLKLLNNLHRERLECIDSLDFKDLKRTF 449
           TELLKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 TELLKLLNNLHRERLECIDSLDFKDLKRTF 450

BLAST of CmoCh04G011070 vs. NCBI nr
Match: XP_023514876.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856.3 bits (2211), Expect = 1.2e-244
Identity = 440/447 (98.43%), Postives = 442/447 (98.88%), Query Frame = 0

Query: 2   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 61
           MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI
Sbjct: 1   MMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANI 60

Query: 62  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQV 121
           ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTP+VPASPVQV
Sbjct: 61  ETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPKVPASPVQV 120

Query: 122 REILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 181
           REILDKIAAAV REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE
Sbjct: 121 REILDKIAAAVGREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPE 180

Query: 182 RFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 241
           RFEKRVEILEEIL TMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE
Sbjct: 181 RFEKRVEILEEILWTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKE 240

Query: 242 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFL 301
           IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFL
Sbjct: 241 IISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFL 300

Query: 302 QMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL 361
           QMGLVPLLLETLVDAERSVCERALGVFDGICETK+GREEAYAHALTMPVIVKKILRVSDL
Sbjct: 301 QMGLVPLLLETLVDAERSVCERALGVFDGICETKRGREEAYAHALTMPVIVKKILRVSDL 360

Query: 362 VTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 421
            TELSVSIVWKLVKHESR E EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL
Sbjct: 361 ATELSVSIVWKLVKHESR-EGEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATEL 420

Query: 422 LKLLNNLHRERLECIDSLDFKDLKRTF 449
           LKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 LKLLNNLHRERLECIDSLDFKDLKRTF 446

BLAST of CmoCh04G011070 vs. NCBI nr
Match: XP_022996865.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 847.8 bits (2189), Expect = 4.2e-242
Identity = 434/448 (96.88%), Postives = 440/448 (98.21%), Query Frame = 0

Query: 1   MMMFSWRRRRATRRMARGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60
           MMMFSWRRRRATRRMAR  GGGGGSM ELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN
Sbjct: 1   MMMFSWRRRRATRRMAR--GGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRAN 60

Query: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120
           IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ
Sbjct: 61  IETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQ 120

Query: 121 VREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180
           VREILDKIAAA  REDCE CKS+VEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP
Sbjct: 121 VREILDKIAAAAGREDCEECKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKP 180

Query: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240
           ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK
Sbjct: 181 ERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLK 240

Query: 241 EIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300
           EIISSYPEKVSELGEIQGVLEG+MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF
Sbjct: 241 EIISSYPEKVSELGEIQGVLEGVMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAF 300

Query: 301 LQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360
           LQMGLV LLLETL+DAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD
Sbjct: 301 LQMGLVSLLLETLIDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSD 360

Query: 361 LVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420
           L TELSVSIVWKLVKHESRE+E++EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE
Sbjct: 361 LATELSVSIVWKLVKHESREDEDEEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATE 420

Query: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 449
           LLKLLNNLHRERLECIDSLDFKDLKRTF
Sbjct: 421 LLKLLNNLHRERLECIDSLDFKDLKRTF 446

BLAST of CmoCh04G011070 vs. NCBI nr
Match: KAG7031489.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.8 bits (2150), Expect = 1.4e-237
Identity = 430/436 (98.62%), Postives = 430/436 (98.62%), Query Frame = 0

Query: 15  MAR-GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 74
           MAR GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60

Query: 75  FTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVR 134
           FTNQPLHTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPVQVREILDKIAAAV 
Sbjct: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120

Query: 135 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 194
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180

Query: 195 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 254
           LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240

Query: 255 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL 314
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLVPLLLETL
Sbjct: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300

Query: 315 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKL 374
           VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL TELSVSIVWKL
Sbjct: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360

Query: 375 VKHESREEE-EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 434
           VKHESREEE EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420

Query: 435 LECIDSLDFKDLKRTF 449
           LECIDSLDFKDLKRTF
Sbjct: 421 LECIDSLDFKDLKRTF 436

BLAST of CmoCh04G011070 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 366.3 bits (939), Expect = 3.5e-101
Identity = 203/423 (47.99%), Postives = 271/423 (64.07%), Query Frame = 0

Query: 27  AELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIP 86
           +E+TIP  F CPIS+DLMKDPV +STGITYDR +IETWI  GN +CP TN  L T D IP
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIP 86

Query: 87  NHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEK 146
           NH IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A RR D E C  ++EK
Sbjct: 87  NHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEK 146

Query: 147 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 206
           IK L  ESE+N+KC+  N  G VL   F+ FS     EK   +L EILS +  +FP+  E
Sbjct: 147 IKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLE 206

Query: 207 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKL 266
            I  L S  S + +   ++  D S R+++  ++KEI+S    +V       GV E L+KL
Sbjct: 207 GISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKL 266

Query: 267 IKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALG 326
           I++ + SSS K+SL   Y  +            FL++GLV + +E +VDAE SVCE+AL 
Sbjct: 267 IRDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALA 326

Query: 327 VFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDED 386
           V D ICET+ GREE   +AL MP++VKKI +VS+L T  S+S++ KL K  +    ED  
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVED-- 386

Query: 387 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSL-DFKDLK 446
                 A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +K
Sbjct: 387 ------AVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYIK 435

Query: 447 RTF 449
           + F
Sbjct: 447 KPF 435

BLAST of CmoCh04G011070 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-85
Identity = 183/425 (43.06%), Postives = 261/425 (61.41%), Query Frame = 0

Query: 28  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 87
           E+TIPS F CPIS +LMKDPV +++GITYDR NIE W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 88  HNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEKI 147
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 148 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 207
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 208 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 267
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 268 KEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALGV 327
                 S+   SL   +H I   +NQE  ++ FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQETVSR-FLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 328 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDEDG 387
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K          DG
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--------SGDG 389

Query: 388 GVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFKD 447
               EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK 
Sbjct: 390 SEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKH 431

Query: 448 LKRTF 449
           +K+ F
Sbjct: 450 VKKPF 431

BLAST of CmoCh04G011070 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 282.7 bits (722), Expect = 5.1e-76
Identity = 173/425 (40.71%), Postives = 248/425 (58.35%), Query Frame = 0

Query: 28  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 87
           E+TIPS F CPIS +LMKDP                W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLEQIPN 89

Query: 88  HNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEKI 147
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA RR D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 148 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 207
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 208 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 267
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 268 KEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETLVDAERSVCERALGV 327
                 S+   SL   +H I   +NQE  ++ FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQETVSR-FLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 328 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDEDG 387
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K          DG
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK--------SGDG 389

Query: 388 GVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFKD 447
               EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK 
Sbjct: 390 SEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFKH 415

Query: 448 LKRTF 449
           +K+ F
Sbjct: 450 VKKPF 415

BLAST of CmoCh04G011070 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 3.1e-65
Identity = 148/348 (42.53%), Postives = 226/348 (64.94%), Query Frame = 0

Query: 106 IERIPTPRVPASPVQVREILDKIAAAVRREDCEGCKSLVEKIKILVQESERNKKCIIANG 165
           IERI +PRV  +P    EI  ++  A  RE+   C  +V KIK L +  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 166 TGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPL-QEEAIKHLKSEASMQALVWFV 225
           +   LSS FE F+     +  + +LEEIL  ++   PL + E    + S AS+  LV F+
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 226 RGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITY 285
            G+D   R+++   ++E+I+     V  L +++G  EGL+K+I++ + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 286 HAIASSSNQEGFTKAFLQMGLVPLLLETLV-DAERSVCERALGVFDGICETKQGREEAYA 345
            AI+S+   +  T+ F+++GLV L+ E +V +AE+SVCER+L V +  C+ +QG+E+   
Sbjct: 220 RAISSN---DKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLR 279

Query: 346 HALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEEEDEDGGVKVEALQVGAFQKLL 405
           +AL +P++VKKILRVSDL T+ SVSI+WKL K++   E +D    + VEALQVGAF+KLL
Sbjct: 280 NALIVPLLVKKILRVSDLATQCSVSILWKLWKNKKDGECDDR---LLVEALQVGAFEKLL 339

Query: 406 LLLQVGCSEWTKEKATELLKLLNNLHR--ERLECID-SLDFKDLKRTF 449
           +LLQVGC + TKEKA+ELL+ LN      E+  C+D S+  K++K++F
Sbjct: 340 VLLQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of CmoCh04G011070 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 206.1 bits (523), Expect = 6.0e-53
Identity = 138/419 (32.94%), Postives = 217/419 (51.79%), Query Frame = 0

Query: 29  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIE-GGNFSCPFTNQPLHTIDSIPN 88
           + IP HF CPISL+LM+DPVT+ TG TYDRA+IE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 89  HNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAV-RREDCEGCKSLVEK 148
           H +R++IQ WCV NR++G+ERIPTP+ PA P  VR +L + +A            + + +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 149 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 208
           ++   ++S++N+  I A+    +L      FS+        E++ E L+ + M+   +  
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITEPN 191

Query: 209 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEI---QGVLEGL 268
               + S+      +  +  +     R +   L EI+S+  +     G I   + V EG+
Sbjct: 192 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGV 251

Query: 269 MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL-VDAERSVCE 328
           + L++ PI   S + +L I    + +  + +      +  G   +L++ L  D +R   E
Sbjct: 252 LDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 311

Query: 329 RALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKLVKHESREEE 388
           RAL   + +C T +G      HALT+P++VK ILRVSD  TE +   +  L   E R  E
Sbjct: 312 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWRE 371

Query: 389 EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDF 442
           E    GV V+         LLL++Q  C+E  K+KA +LLKLL +   +     +S DF
Sbjct: 372 EAAGAGVVVQ---------LLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5PNY64.9e-10047.99U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D13.8e-8443.06U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q9LT798.5e-5232.94U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA46.1e-5033.17U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9SVC69.7e-4829.95E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FPJ64.1e-251100.00RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642... [more]
A0A6J1K7Y62.0e-24296.88RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 P... [more]
A0A0A0KMI85.1e-20984.22RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
A0A1S3CPH43.4e-20583.11RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ... [more]
A0A6J1K8U95.1e-20180.98RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491172 P... [more]
Match NameE-valueIdentityDescription
XP_022942676.18.5e-251100.00U-box domain-containing protein 21-like [Cucurbita moschata][more]
KAG6600854.11.1e-24598.67U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_023514876.11.2e-24498.43U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
XP_022996865.14.2e-24296.88U-box domain-containing protein 21-like [Cucurbita maxima][more]
KAG7031489.11.4e-23798.62U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
AT5G37490.13.5e-10147.99ARM repeat superfamily protein [more]
AT1G66160.12.7e-8543.06CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.25.1e-7640.71CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.13.1e-6542.53ARM repeat superfamily protein [more]
AT3G19380.16.0e-5332.94plant U-box 25 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 34..97
e-value: 2.8E-28
score: 110.0
IPR003613U box domainPFAMPF04564U-boxcoord: 31..102
e-value: 6.2E-20
score: 71.2
IPR003613U box domainPROSITEPS51698U_BOXcoord: 30..104
score: 35.570324
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 24..108
e-value: 7.6E-28
score: 98.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 120..427
e-value: 4.3E-14
score: 54.3
NoneNo IPR availablePANTHERPTHR22849:SF139ARM REPEAT SUPERFAMILY PROTEINcoord: 12..448
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 12..448
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 35..77
e-value: 2.2445E-22
score: 87.6742
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 23..107
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 124..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G011070.1CmoCh04G011070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity