CmoCh04G009470 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G009470
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.3-like
LocationCmo_Chr04: 4827783 .. 4831214 (-)
RNA-Seq ExpressionCmoCh04G009470
SyntenyCmoCh04G009470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCAAACAAACACTAAGCCTACCAAATGGAAATGGAAATGGATATGGATATGGACATTGAGAGAAGGAGGGACACCCATCTCATGCCTTCTCAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCGGTCGCTCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGTAACGACCCTCTTTCTCTCTCGAGCCTTTCTTGAATTCTACATCATTCTTAAGTGTTGCTGACGTGACTGACAATGAATAGAAATAGGATATGAAAATAAAAAAGTTCTCGTTTCTCTTATTTTTTTTATATTGATTTTGGGTTTGATCGGTTTAAAGGGTATGGCTTGAATTTTTTTTAAAATGGTTCTAAAAGCTTTTTTCGAGGTGGGTTTTAGGAGAGAAAAATAAGCATCATAAAAGGTTATAGTTTTGAATCTCGATCTCGTATATTTAACTTGTGATAATTTTGAATCTAATAGAGCGATCGCTTTGTATACGGTCCGTACAGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGTAAGTTCCCCCTTCATCGCGTTTGTTTATCGTATACTTGTGGTTTGAATCCAAAACATCTTTATCTCTCGATCGATGATATATATTTTTACTAGTTGAGTTATATTTAGGTTCACCCATGATAGCCAACACATATATAATCTCGAAAAAATACAGTTTTTGCATGTGCATGCATACATGATAGATTAGCTAAGAAAAAAAGGTGTTATAAATAATGGTAAAAAGTGTGTTGTTTTAGGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATCACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTTTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTCTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGGGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCATTTTTATCCCAATTCTTCAAGTAATTTTCTTTCTTTACAACTTTACATGTGTTGGTATTGTGCACACTTACCATGAAACTCTGTGTCCTTCAGAAAAAGCTTTCTTTTGTGTCTCAAACATGTTCCCTAAACCATATATCCCTTTTCTTTTCCTTTTTATACTTTCTTCTGGTTATTATCTTCCTTTAATCCTTTCCTTTTAAATCGTCTTCTTGTTTGAGTATAATTTCCGTCTTCTTTTTATAAGAATCTTAAACTATATGTTCATCAAACATGCGATATGAAAGTTTATCGAAGAAGAAAAAAGCGGGATTTTGTGTTGAAAGTGTTGTGTTACTAAAATAGAATTGAAATCTGCTAGGAATGTTGAGATTTTATTTTTCCATTTTTGAGCTAAAGAAATAATGGGAATTTGAAAGTAAACAATTTAGGGTCATAGTGGGGAGACTATTTGAAAATAATGTGGTTATGTTGATTAAAAAAGGAGTAACATTTATGTATGAATTGAAAAGACATAAAAGTTAGTGCTGTCTGGTGAGTCATTTTGTCCTGAAAAAGTGGGATCAAAACTACTTTATTTCTCATACTAATCCAAAGAAAATATATATGTTTTTCAGGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGTCCTCTTTCTTTTCTAGTTTGAACTTGATTTGGACGTCTCAAAGGACTTCACAACATAACCTTAAGAGTTCCCTTAAACAAGCTCAAATCTTTTGAACATAAAAGCCAAATTCTCTATCAAAATTTGAAGTTTTGGACATGGTGATGAAGTTTTGTACCATAATTTAACTTCAGGTTCTTGGACAAGGCTTGTATCAAAAGCCAAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCTCACTCAAGTAGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGCCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGAAGAGATTCAGCTGTGAATTCGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAGATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACATATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGCTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCATCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACTCACATTTACAACAACAATTTTTCAAAGAACAATGTTGATGGTCATTATGTACCTGGATTAAATGATTTTTCAGTCTACCTTTTAAAAAAAAAGACAATTAGACAATACAGCCTAGACCACTTGGGGAGTTGATAGAAGGAATATAAGTAATCTATTTGATATATATGTATTTAGGGTTTAGGGTTTGTGTGATATATGTGTACTTCTGTAGAGAAGAATCAAAATAATATTAAAAGTAGAAGTCCCCTGTAAATGCCTCGTTACCTAAGTGTAGCAGC

mRNA sequence

CCTCAAACAAACACTAAGCCTACCAAATGGAAATGGAAATGGATATGGATATGGACATTGAGAGAAGGAGGGACACCCATCTCATGCCTTCTCAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCGGTCGCTCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATCACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTTTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTCTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGGGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCATTTTTATCCCAATTCTTCAAGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGTTCTTGGACAAGGCTTGTATCAAAAGCCAAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCTCACTCAAGTAGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGCCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGAAGAGATTCAGCTGTGAATTCGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAGATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACATATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGCTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCATCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACTCACATTTACAACAACAATTTTTCAAAGAACAATGTTGATGGTCATTATGTACCTGGATTAAATGATTTTTCAGTCTACCTTTTAAAAAAAAAGACAATTAGACAATACAGCCTAGACCACTTGGGGAGTTGATAGAAGGAATATAAGTAATCTATTTGATATATATGTATTTAGGGTTTAGGGTTTGTGTGATATATGTGTACTTCTGTAGAGAAGAATCAAAATAATATTAAAAGTAGAAGTCCCCTGTAAATGCCTCGTTACCTAAGTGTAGCAGC

Coding sequence (CDS)

ATGGAAATGGAAATGGATATGGATATGGACATTGAGAGAAGGAGGGACACCCATCTCATGCCTTCTCAGGATCTCACTGTTGATTGGAGAGGAAGACCCTCCAACCCGGTCGCTCACGGCGGGATGAGAGCCGCTGCTTTTGTTCTCGGGCTGCAATCATTCGAGATAATGGCGATTGCTGCAGTTGGGAACAATCTGATAACATATGTGATAAATGAGATGCACTTTTCACTGTCAAAATCTGCAAACGTAGTGACAAACTTTGTTGGGACAGTGTTTCTTTTGGCACTTCTTGGCGGTTTCCTTTCTGATTCCTATCTTGGGAGTTTTTGGACAATGCTTATCTTTGGCTTTGTTGAACTTTCTGGGTTCATATTACTATCAGTTCAGGCTCATCTTCCCCAACTAAAGCCACCCCAATGCAACATGCTAAGTGAAGGAGATCACTGCGTGGAAGCCAAGGGGATAAAGGCATTGATATTCTTTGTGGCACTTTACTTGGTGGCTCTTGGGAGTGGGTGTGTGAAGCCCAACATGATAGCTCATGGGGCTGATCAGTTCACTCCACAAGATCCAAGCCAATCCAAGAGGCTCTCAACATACTTCAATGCTGCTTATTTTGCCTTCTCAATTGGGGAGCTCATTGCCCTCACTCTTGTTGTTTGGATCCAAACCCATTCTGGGATGGACGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCTATGGGGTTGATCAGCTTGGTTGGCGGTATTTTTTACTACAGGAATAAACCCCCTCAAGGAGCCATTTTTATCCCAATTCTTCAAGTCTTTGTTGCCGCTGTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAACCCTCAAATGCTTCATGGAAGCCAAACCAGTAATCATAAAGTTGCCTTATCCTCTCATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGTTCTTGGACAAGGCTTGTATCAAAAGCCAAGATGGAACAACCAGGGAAAGCCCATGGAGATTGTGCACTCTCACTCAAGTAGAGCAAGTGAAGATATTGATATCAGTTGTCCCAATATTTGCTTGCACGATTATATTCAACACAATATTGGCTCAGCTCCAAACATTTTCTGTTCAACAAGGGAGTTTGATGAATACTCAGCTCACCAAATCCTTCCACATTCCTCCCGCTTCCCTTCAGTCCATTCCTTACATTATGCTCATTTTCATTGTCCCTTTGTATGATACTTTCTTTGTCCCATTTGCTAGAAAGTTCACTGGCCACACCTCTGGAATCTCACCCTTAAAACGTATTGGCTTTGGCCTCTTCCTCTCCACATTTTCGATGGTTTCAGCAGCTATAATGGAGAAAAAGAGAAGAGATTCAGCTGTGAATTCGAACCAGACGATATCGATCTTCTGGATCACACCACAGTTCCTTATTTTTGGATTGTCAGAGATGTTCACAGCAGTTGGACTCATTGAGTTCTTTTACAAGCAATCAGTAAAAGGGATGCAAGCATTTTTAACAGCCATAACATATTGCTCATACTCATTTGGGTTTTACTTGAGCTCATTGCTAGTGTCCATGGTGAATAAGATCACATCAAGCTCCACCAACGGAGGCTGGCTCCATGACAACAACCTAAACAAAGACAGGCTTGATCTCTTCTACTGGTTGCTGGCTGCACTTAGCTTCCTCAACTTTCTTAACTATCTCTTCTGGTCCAGTTGGTTCCAAGATCCCCCATCTCTCTCCCAAAAGCTACAACAAGATAATCTTCACCATGGAGAAAACTCACATTTACAACAACAATTTTTCAAAGAACAATGTTGA

Protein sequence

MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQFFKEQC
Homology
BLAST of CmoCh04G009470 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-239
Identity = 421/554 (75.99%), Postives = 482/554 (87.00%), Query Frame = 0

Query: 22  SQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           +++ +VDWRGRPSNP  HGGMRAA FVLGLQ+FEIM IAAVGNNLITYVINEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN+VTNFVGT+F+ ALLGG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 142 NMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N L +   C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+   P QSKRLS+Y
Sbjct: 145 NPLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSY 204

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+ 
Sbjct: 205 FNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRR 264

Query: 262 AIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIP-TQKFRFLDKA 321
           +IF PI  V VAA+LKRK   PS+P+MLHG    +H VA     ++ +P T +FRFLDKA
Sbjct: 265 SIFTPIAHVIVAAILKRKLASPSDPRMLHG----DHHVANDVVPSSTLPHTPRFRFLDKA 324

Query: 322 CIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 381
           CIK QD  T+ESPWRLCT+TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 325 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 384

Query: 382 TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 441
           T+L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 385 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 444

Query: 442 TFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 501
           TFSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM
Sbjct: 445 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 504

Query: 502 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG-GWLHDNNLNKDRLDLFYWLLAAL 561
           ++FL A+TYCSYSFGFY SS+LVS+VNKITS+S +  GWL +N+LNKDRLDLFYWLLA L
Sbjct: 505 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 564

Query: 562 SFLNFLNYLFWSSW 574
           S LNFL+YLFWS W
Sbjct: 565 SLLNFLSYLFWSRW 573

BLAST of CmoCh04G009470 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.8e-229
Identity = 409/579 (70.64%), Postives = 479/579 (82.73%), Query Frame = 0

Query: 24  DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSAN 83
           DL VD+RGRP  P  HGG RAA FVLG Q+FE+MAIAAVGNNLITYV NEMHF LSKSAN
Sbjct: 24  DLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSAN 83

Query: 84  VVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNM 143
           +VTNF+GTVFLL+LLGGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+PP+CNM
Sbjct: 84  LVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNM 143

Query: 144 LSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFN 203
            S   HCVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +D    ++LS++FN
Sbjct: 144 KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFN 203

Query: 204 AAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAI 263
           AAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRNKPP G+I
Sbjct: 204 AAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSI 263

Query: 264 FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIK 323
           F PI QVFVAA+ KRKQ+CPSNP M+H   T   +V    HS       KFRFLDKACIK
Sbjct: 264 FTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHS------NKFRFLDKACIK 323

Query: 324 SQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQL 383
           +Q G   ESPWRLCT+ QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQGS MNT +
Sbjct: 324 TQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 383

Query: 384 TKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFS 443
           TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+TFS
Sbjct: 384 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 443

Query: 444 MVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAF 503
           MV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQS + MQ+F
Sbjct: 444 MVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSF 503

Query: 504 LTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG---GWLHDNNLNKDRLDLFYWLLAALS 563
           LTA+TYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+LS
Sbjct: 504 LTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLS 563

Query: 564 FLNFLNYLFWSSWFQ-DPPSLSQKLQQDNLHHGENSHLQ 599
           F+NF NYLFWS W+  DP +     + ++L   EN  ++
Sbjct: 564 FINFFNYLFWSRWYSCDPSATHHSAEVNSLEALENGEIK 592

BLAST of CmoCh04G009470 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 7.5e-112
Identity = 239/564 (42.38%), Postives = 341/564 (60.46%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+GT FLLALLGGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 148 DHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPI 267
             + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 268 LQVFVAAVLK-RKQVCPSNPQMLHGSQTSNH-----KVALSSHSANLIPTQKFRFLDKA- 327
           L+V +AA +K       SN         SNH     K  + S      P Q+     +A 
Sbjct: 255 LKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQ 314

Query: 328 ---CIKSQDGTTRESP-WRL--CTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQ 387
               +K  +G   E P  RL  CT+ QVE VKI++ ++PIFACTI+ N  LAQL TFSVQ
Sbjct: 315 LTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQ 374

Query: 388 QGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIG 447
           Q + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+RIG
Sbjct: 375 QAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIG 434

Query: 448 FGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVG 507
            GL LS  +M  AA++E KR+  A     ++S +T  ++  WI  Q+L  G +++FT  G
Sbjct: 435 VGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAG 494

Query: 508 LIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKD 567
           L+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N+ 
Sbjct: 495 LLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRY 554

Query: 568 RLDLFYWLLAALSFLNFLNYLFWS 572
           +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 KLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmoCh04G009470 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 2.8e-106
Identity = 224/555 (40.36%), Postives = 333/555 (60.00%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+ T FLLALLGGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS-SAA 136

Query: 148 DHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C    G KA   FV LYLV+LG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPI 267
             S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G+    I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 268 LQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIKSQDG 327
            +V +AA +       S+         S H+    S S     T     L+KA     +G
Sbjct: 257 FKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL----TNSLTCLNKAI----EG 316

Query: 328 TTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTKSF 387
            T    W  CT+ QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 317 KTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 376

Query: 388 HIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSA 447
           ++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 377 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 436

Query: 448 AIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 507
           A++E KR+  A     ++S +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 437 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 496

Query: 508 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALS 567
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 497 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 556

Query: 568 FLNFLNYLFWSSWFQ 576
            +NFL+YLFW+  ++
Sbjct: 557 VVNFLHYLFWAKRYK 559

BLAST of CmoCh04G009470 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 1.4e-105
Identity = 215/563 (38.19%), Postives = 339/563 (60.21%), Query Frame = 0

Query: 22  SQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           +QD TVD    P+N    G  +A  F+LG +  E +A   +G NL+ Y+ + ++   + +
Sbjct: 8   TQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATA 67

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN VTN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A +P LKP  C
Sbjct: 68  ANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNC 127

Query: 142 NMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N     D C      +  +FFVALY++ALG+G +KP + + GADQF   D ++  + S++
Sbjct: 128 N----ADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSF 187

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FN  YF+ ++G LIA T++VWIQ + G   GFGV    M + +     G  +YR + P G
Sbjct: 188 FNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGG 247

Query: 262 AIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKAC 321
           +    I QV VAA  K     P +  +L  +      +     S  L+ T   +F DKA 
Sbjct: 248 SPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNI---KGSRKLVHTDNLKFFDKAA 307

Query: 322 IKSQDGTTRE---SPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSL 381
           ++SQ  + ++   +PWRLC++TQVE++K +I+++P++A  I+F T+ +Q+ T  V QG+ 
Sbjct: 308 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 367

Query: 382 MNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLF 441
           M+  + K+F IP ASL     + ++F  P+YD F +P ARKFT +  G + L+R+G GL 
Sbjct: 368 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 427

Query: 442 LSTFSMVSAAIMEKKRRDSAVNSNQ------TISIFWITPQFLIFGLSEMFTAVGLIEFF 501
           +S F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EFF
Sbjct: 428 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 487

Query: 502 YKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLF 561
           Y Q+   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD F
Sbjct: 488 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYF 547

Query: 562 YWLLAALSFLNFLNYLFWSSWFQ 576
           ++LLA LSFLNFL YL+ S  ++
Sbjct: 548 FYLLATLSFLNFLVYLWISKRYK 561

BLAST of CmoCh04G009470 vs. ExPASy TrEMBL
Match: A0A6J1FR74 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 PE=3 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60
           MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA
Sbjct: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60

Query: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120
           AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE
Sbjct: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120

Query: 121 LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 180
           LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI
Sbjct: 121 LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 180

Query: 181 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 240
           AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM
Sbjct: 181 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 240

Query: 241 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 300
           AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA
Sbjct: 241 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 300

Query: 301 LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII 360
           LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII
Sbjct: 301 LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII 360

Query: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420
           FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF
Sbjct: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420

Query: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 480
           TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS
Sbjct: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 480

Query: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 540
           EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH
Sbjct: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 540

Query: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ 600
           DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ
Sbjct: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ 600

Query: 601 FFKEQC 607
           FFKEQC
Sbjct: 601 FFKEQC 606

BLAST of CmoCh04G009470 vs. ExPASy TrEMBL
Match: A0A6J1JRW4 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE=3 SV=1)

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 595/604 (98.51%), Postives = 599/604 (99.17%), Query Frame = 0

Query: 3   MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEM+M+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 1   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 61  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 121 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 181 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 240

Query: 243 GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 241 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 300

Query: 303 SHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFRFLDKACIKS DGTTRESPWRLCT TQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 480

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 540

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHHGEN HLQQQFF
Sbjct: 541 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 600

Query: 603 KEQC 607
           KEQC
Sbjct: 601 KEQC 604

BLAST of CmoCh04G009470 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 7.2e-299
Identity = 529/601 (88.02%), Postives = 560/601 (93.18%), Query Frame = 0

Query: 9   MDIERRRD----------THLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMA 68
           M++ERRRD           + MPS+D+TVDWRGRPSN V+HGGMRAAAFVLGLQ+FEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 69  IAAVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGF 128
           IAAVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 129 VELSGFILLSVQAHLPQLKPPQCNML-SEGDHCVEAKGIKALIFFVALYLVALGSGCVKP 188
           VELSGFILLSVQAH+PQLKPPQCNML S+G+ CVEAKG+KALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 189 NMIAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSA 248
           NMIAHGADQF+PQ P+QSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSA
Sbjct: 181 NMIAHGADQFSPQSPTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSA 240

Query: 249 AVMAMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNH 308
           AVMA+GLISLV GIFYYRNKPPQG IF PILQVFVAA+LKRKQVCPSNP MLHGSQTSN 
Sbjct: 241 AVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND 300

Query: 309 KVALSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFAC 368
           KV LSSHSANLIPTQKFRFL+KACIK+QD   +ESPWRLCT  QVEQVKIL+SVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 369 TIIFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFA 428
           TI+FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 429 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIF 488
           RKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSAVN NQTISIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 489 GLSEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGG 548
           GLSEMFTAVGLIEFFY QS+KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGG
Sbjct: 481 GLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 549 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQ-QDNLHHGENSH 598
           WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWS WF + PS+SQK Q  DN HHGE+ H
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

BLAST of CmoCh04G009470 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1033.1 bits (2670), Expect = 4.7e-298
Identity = 528/599 (88.15%), Postives = 557/599 (92.99%), Query Frame = 0

Query: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60
           MEME   D   E   + + MPS+D+TVDWRGRPSN V+HGGMRAAAFVLGLQ+FEIMAIA
Sbjct: 1   MEMERRRDFKGENNANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIA 60

Query: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120
           AVGNNLITY+INEMHFSLSKSAN+VTNFVGTVFLLAL+GGFLSDSYLGSFWTMLIFGFVE
Sbjct: 61  AVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVE 120

Query: 121 LSGFILLSVQAHLPQLKPPQCNML-SEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNM 180
           LSGFILLSVQAH+PQLKPPQCNML S+G+ CVEAKG+KALIFFVALYLVALGSGCVKPNM
Sbjct: 121 LSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNM 180

Query: 181 IAHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAV 240
           IAHGADQF+PQ PSQSK LSTYFNAAY AFSIGELIALTL+VWIQTHSGMDVGFGVSAAV
Sbjct: 181 IAHGADQFSPQSPSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAAV 240

Query: 241 MAMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKV 300
           MA+GLISLV GIFYYRNKPPQG IF PILQVFVAA+LKRKQVCPSNP MLHGSQTSN KV
Sbjct: 241 MALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSNDKV 300

Query: 301 ALSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTI 360
            LSSHSANLIPTQKFRFL+KACIK+QD   +ESPWRLCT  QVEQVKIL+SVVPIFACTI
Sbjct: 301 GLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFACTI 360

Query: 361 IFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARK 420
           +FNTILAQLQTFSVQQGSLMNTQLTKS HIPPASLQSIPYIMLI IVPLYDTFFVPFARK
Sbjct: 361 VFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFARK 420

Query: 421 FTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGL 480
            TGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSAVN NQTISIFWITPQFLIFGL
Sbjct: 421 ITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIFGL 480

Query: 481 SEMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWL 540
           SEMFTAVGLIEFFYKQS+KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGGWL
Sbjct: 481 SEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWL 540

Query: 541 HDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQ-QDNLHHGENSHL 598
           HDNNLNKDRLDLFYWLLAALSFLN LNYLFWS WF + PS+SQK Q  DN HHGE+ H+
Sbjct: 541 HDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYHI 599

BLAST of CmoCh04G009470 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.4e-297
Identity = 531/595 (89.24%), Postives = 563/595 (94.62%), Query Frame = 0

Query: 7   MDMDIERRRDTHLMPSQD-LTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNN 66
           M+M++ERR+D      +D +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNN
Sbjct: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60

Query: 67  LITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFI 126
           LITY INEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTML+FGFVELSGFI
Sbjct: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120

Query: 127 LLSVQAHLPQLKPPQCNMLSE-GDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 186
           LLSVQAHLPQLKPPQCNMLSE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 187 DQFTPQ-DPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 246
           DQF+PQ +P  SK+LSTYFNAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240

Query: 247 LISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALS 306
           LISLVGGIFYYRNKPPQG IF PILQVFVAA+LKRKQVCPSNP MLHGSQ + +HKVALS
Sbjct: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300

Query: 307 SHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFN 366
           S SANLIPTQKFRFLDKACIKSQDG T+ESPWRLCT TQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 367 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 426
           TILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 427 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 486
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA+N NQT+SIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480

Query: 487 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 546
           FTAVGLIEFFYKQS KGMQAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540

Query: 547 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQD-NLHHGENSH 597
           NLNKDRLDLFYWLLA LSFLNFLNYLFWS+WF + PSLSQK   D N HHGE+ H
Sbjct: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 595

BLAST of CmoCh04G009470 vs. NCBI nr
Match: XP_022942404.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita moschata])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60
           MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA
Sbjct: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60

Query: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120
           AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE
Sbjct: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120

Query: 121 LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 180
           LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI
Sbjct: 121 LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 180

Query: 181 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 240
           AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM
Sbjct: 181 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 240

Query: 241 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 300
           AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA
Sbjct: 241 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 300

Query: 301 LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII 360
           LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII
Sbjct: 301 LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII 360

Query: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420
           FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF
Sbjct: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420

Query: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 480
           TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS
Sbjct: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 480

Query: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 540
           EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH
Sbjct: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 540

Query: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ 600
           DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ
Sbjct: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ 600

Query: 601 FFKEQC 607
           FFKEQC
Sbjct: 601 FFKEQC 606

BLAST of CmoCh04G009470 vs. NCBI nr
Match: KAG6600707.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031346.1 Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1171.4 bits (3029), Expect = 0.0e+00
Identity = 600/606 (99.01%), Postives = 602/606 (99.34%), Query Frame = 0

Query: 1   MEMEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 60
           MEMEM+MDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA
Sbjct: 3   MEMEMEMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIA 62

Query: 61  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 120
           AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE
Sbjct: 63  AVGNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVE 122

Query: 121 LSGFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 180
           LSGFILLSVQAHLPQLKPPQCNM+SEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI
Sbjct: 123 LSGFILLSVQAHLPQLKPPQCNMVSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMI 182

Query: 181 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 240
           AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM
Sbjct: 183 AHGADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVM 242

Query: 241 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 300
           AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA
Sbjct: 243 AMGLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVA 302

Query: 301 LSSHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTII 360
           LSSHSANLIPTQK  FLDKACIKSQDGTTRESPWRLCT TQVEQVKILISVVPIFACTII
Sbjct: 303 LSSHSANLIPTQK--FLDKACIKSQDGTTRESPWRLCTPTQVEQVKILISVVPIFACTII 362

Query: 361 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 420
           FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF
Sbjct: 363 FNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKF 422

Query: 421 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 480
           TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS
Sbjct: 423 TGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLS 482

Query: 481 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 540
           EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH
Sbjct: 483 EMFTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLH 542

Query: 541 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQ 600
           DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHH ENSHLQQQ
Sbjct: 543 DNNLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHEENSHLQQQ 602

Query: 601 FFKEQC 607
           FFKEQC
Sbjct: 603 FFKEQC 606

BLAST of CmoCh04G009470 vs. NCBI nr
Match: XP_022993122.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita maxima])

HSP 1 Score: 1167.9 bits (3020), Expect = 0.0e+00
Identity = 595/604 (98.51%), Postives = 599/604 (99.17%), Query Frame = 0

Query: 3   MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEM+M+MDIERRRDTHLMPS+DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 1   MEMEMEMDIERRRDTHLMPSEDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 60

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 61  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 120

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGD CVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 121 GFILLSVQAHLPQLKPPQCNMLSEGDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 180

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 181 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 240

Query: 243 GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGA+FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 241 GLISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 300

Query: 303 SHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFRFLDKACIKS DGTTRESPWRLCT TQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 480

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 540

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPP LSQKLQQDNLHHGEN HLQQQFF
Sbjct: 541 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPHLQQQFF 600

Query: 603 KEQC 607
           KEQC
Sbjct: 601 KEQC 604

BLAST of CmoCh04G009470 vs. NCBI nr
Match: XP_023530877.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 590/602 (98.01%), Postives = 594/602 (98.67%), Query Frame = 0

Query: 3   MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 62
           MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV
Sbjct: 5   MEMDMDMDIERRRDTHLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAV 64

Query: 63  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 122
           GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS
Sbjct: 65  GNNLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELS 124

Query: 123 GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 182
           GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH
Sbjct: 125 GFILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAH 184

Query: 183 GADQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 242
           GADQFTPQ PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM
Sbjct: 185 GADQFTPQHPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAM 244

Query: 243 GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 302
           GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS
Sbjct: 245 GLISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALS 304

Query: 303 SHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFN 362
           SHSANLIPTQKFRFLDKAC+KSQDGTTRESPWRLCT TQVEQVKILISVVPIFACTIIFN
Sbjct: 305 SHSANLIPTQKFRFLDKACVKSQDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 364

Query: 363 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 422
           TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 365 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 424

Query: 423 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 482
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAV+SNQTISIFWITPQFLIFGLSEM
Sbjct: 425 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVDSNQTISIFWITPQFLIFGLSEM 484

Query: 483 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 542
           FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN
Sbjct: 485 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 544

Query: 543 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENSHLQQQFF 602
           NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGEN H+    F
Sbjct: 545 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQDNLHHGENXHIYNNNF 604

Query: 603 KE 605
            +
Sbjct: 605 SK 606

BLAST of CmoCh04G009470 vs. NCBI nr
Match: XP_038903187.1 (protein NRT1/ PTR FAMILY 4.3-like [Benincasa hispida])

HSP 1 Score: 1042.7 bits (2695), Expect = 1.2e-300
Identity = 532/591 (90.02%), Postives = 561/591 (94.92%), Query Frame = 0

Query: 9   MDIERRRD----THLMPSQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGN 68
           M++ER+RD    +H +PS+D++VDWRGRPSNP +HGGMRAAAFVLGLQ+FEIMAIAAVGN
Sbjct: 1   MEMERKRDFKGESHTIPSEDVSVDWRGRPSNPTSHGGMRAAAFVLGLQAFEIMAIAAVGN 60

Query: 69  NLITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGF 128
           NLITY+INEMHFSLSKSAN+VTNFVGTVFLLALLGGFLSDSYLGSFWT+LIFGFVELSGF
Sbjct: 61  NLITYLINEMHFSLSKSANIVTNFVGTVFLLALLGGFLSDSYLGSFWTILIFGFVELSGF 120

Query: 129 ILLSVQAHLPQLKPPQCNMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 188
           ILLSVQAH+PQLKPPQCNML + D CVEAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 ILLSVQAHVPQLKPPQCNMLLKSDECVEAKGVKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 189 DQFTPQDPSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGL 248
           DQF+PQ+ SQSK LS YFNAAYFAFSIGELIALTL+VWIQTHSGMDVGFGVSAAVMA+GL
Sbjct: 181 DQFSPQNSSQSKSLSKYFNAAYFAFSIGELIALTLLVWIQTHSGMDVGFGVSAAVMALGL 240

Query: 249 ISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSH 308
           I LV GIFYYRNKPPQG IFIPILQVFVAA+LKRKQVCPSN  MLHGSQTSN KVALSS 
Sbjct: 241 IILVAGIFYYRNKPPQGPIFIPILQVFVAALLKRKQVCPSNSHMLHGSQTSNDKVALSSD 300

Query: 309 SANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTI 368
           SANLIPTQKFRFLDKAC KSQDG T+ESPWRLCT  QVEQVKILISVVPIFACTIIFNTI
Sbjct: 301 SANLIPTQKFRFLDKACFKSQDGATKESPWRLCTPNQVEQVKILISVVPIFACTIIFNTI 360

Query: 369 LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT 428
           LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT
Sbjct: 361 LAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHT 420

Query: 429 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFT 488
           SGISPLKRIGFGLFLSTFSMVSAAIMEK+RRDSAVN NQT+SIFWITPQFLIFGLSEMFT
Sbjct: 421 SGISPLKRIGFGLFLSTFSMVSAAIMEKRRRDSAVNLNQTMSIFWITPQFLIFGLSEMFT 480

Query: 489 AVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNL 548
           AVGLIEFFYKQSVKGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKITSSS+NGGWLHDNNL
Sbjct: 481 AVGLIEFFYKQSVKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGGWLHDNNL 540

Query: 549 NKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQ-QDNLHHGEN 595
           NKDRLDLFYWLLAALSFLNFLNYLFWS+WF + PSLSQKLQ  DN H GE+
Sbjct: 541 NKDRLDLFYWLLAALSFLNFLNYLFWSTWFDEHPSLSQKLQNDDNPHQGED 591

BLAST of CmoCh04G009470 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 830.1 bits (2143), Expect = 1.2e-240
Identity = 421/554 (75.99%), Postives = 482/554 (87.00%), Query Frame = 0

Query: 22  SQDLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKS 81
           +++ +VDWRGRPSNP  HGGMRAA FVLGLQ+FEIM IAAVGNNLITYVINEMHF LSK+
Sbjct: 25  AEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKA 84

Query: 82  ANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQC 141
           AN+VTNFVGT+F+ ALLGG+LSD++LGSFWT++IFGFVELSGFILLSVQAHLPQLKPP+C
Sbjct: 85  ANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKC 144

Query: 142 NMLSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTY 201
           N L +   C EAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF+   P QSKRLS+Y
Sbjct: 145 NPLID-QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSY 204

Query: 202 FNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQG 261
           FNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+ISLV G  Y+RNK P+ 
Sbjct: 205 FNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRR 264

Query: 262 AIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIP-TQKFRFLDKA 321
           +IF PI  V VAA+LKRK   PS+P+MLHG    +H VA     ++ +P T +FRFLDKA
Sbjct: 265 SIFTPIAHVIVAAILKRKLASPSDPRMLHG----DHHVANDVVPSSTLPHTPRFRFLDKA 324

Query: 322 CIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMN 381
           CIK QD  T+ESPWRLCT+TQVEQVK LIS+VPIFA TI+FNTILAQLQTFSVQQGS MN
Sbjct: 325 CIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMN 384

Query: 382 TQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLS 441
           T+L+ SFHIPPASLQ+IPYIMLIF+VPLYD+F VPFARK TGH SGI PL RIG GLFLS
Sbjct: 385 TRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLS 444

Query: 442 TFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 501
           TFSMVSAA++EKKRRDS+V   + +SIFWITPQFLIFG+SEMFTAVGLIEFFYKQS KGM
Sbjct: 445 TFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGM 504

Query: 502 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG-GWLHDNNLNKDRLDLFYWLLAAL 561
           ++FL A+TYCSYSFGFY SS+LVS+VNKITS+S +  GWL +N+LNKDRLDLFYWLLA L
Sbjct: 505 ESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVL 564

Query: 562 SFLNFLNYLFWSSW 574
           S LNFL+YLFWS W
Sbjct: 565 SLLNFLSYLFWSRW 573

BLAST of CmoCh04G009470 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 795.0 bits (2052), Expect = 4.1e-230
Identity = 409/579 (70.64%), Postives = 479/579 (82.73%), Query Frame = 0

Query: 24  DLTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSAN 83
           DL VD+RGRP  P  HGG RAA FVLG Q+FE+MAIAAVGNNLITYV NEMHF LSKSAN
Sbjct: 24  DLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSAN 83

Query: 84  VVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNM 143
           +VTNF+GTVFLL+LLGGFLSDSYLGSF TML+FG +E+SGFILLSVQAHLP+L+PP+CNM
Sbjct: 84  LVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNM 143

Query: 144 LSEGDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFN 203
            S   HCVEA G KA   + AL LVALGSGC+KPN+I+HGA+QF  +D    ++LS++FN
Sbjct: 144 KSTTIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFN 203

Query: 204 AAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAI 263
           AAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+ISLV G  +YRNKPP G+I
Sbjct: 204 AAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSI 263

Query: 264 FIPILQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIK 323
           F PI QVFVAA+ KRKQ+CPSNP M+H   T   +V    HS       KFRFLDKACIK
Sbjct: 264 FTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHS------NKFRFLDKACIK 323

Query: 324 SQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQL 383
           +Q G   ESPWRLCT+ QV QVKIL+SV+PIFACTIIFNTILAQLQTFSVQQGS MNT +
Sbjct: 324 TQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHI 383

Query: 384 TKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFS 443
           TK+F IPPASLQ+IPYI+LIF VPLY+TFFVP ARK TG+ SGISPL+RIG GLFL+TFS
Sbjct: 384 TKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFS 443

Query: 444 MVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGMQAF 503
           MV+AA++EKKRR+S +  N  +SIFWI PQFLIFGLSEMFTAVGL+EFFYKQS + MQ+F
Sbjct: 444 MVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSF 503

Query: 504 LTAITYCSYSFGFYLSSLLVSMVNKITSSSTNG---GWLHDNNLNKDRLDLFYWLLAALS 563
           LTA+TYCSYSFGFYLSS+LVS VN++TSS+ +G   GWL DN+LNKDRLD FYWLLA+LS
Sbjct: 504 LTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLS 563

Query: 564 FLNFLNYLFWSSWFQ-DPPSLSQKLQQDNLHHGENSHLQ 599
           F+NF NYLFWS W+  DP +     + ++L   EN  ++
Sbjct: 564 FINFFNYLFWSRWYSCDPSATHHSAEVNSLEALENGEIK 592

BLAST of CmoCh04G009470 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 406.0 bits (1042), Expect = 5.4e-113
Identity = 239/564 (42.38%), Postives = 341/564 (60.46%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y+   MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+GT FLLALLGGFLSD++  +F   LI   +E  G I+L++QA  P L PP C    + 
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC----DS 134

Query: 148 DHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C E  G KA + FV LYLVALG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 135 PTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVF 194

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPI 267
             + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G+    I
Sbjct: 195 CLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTI 254

Query: 268 LQVFVAAVLK-RKQVCPSNPQMLHGSQTSNH-----KVALSSHSANLIPTQKFRFLDKA- 327
           L+V +AA +K       SN         SNH     K  + S      P Q+     +A 
Sbjct: 255 LKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQ 314

Query: 328 ---CIKSQDGTTRESP-WRL--CTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQ 387
               +K  +G   E P  RL  CT+ QVE VKI++ ++PIFACTI+ N  LAQL TFSVQ
Sbjct: 315 LTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQ 374

Query: 388 QGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIG 447
           Q + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L+RIG
Sbjct: 375 QAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIG 434

Query: 448 FGLFLSTFSMVSAAIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVG 507
            GL LS  +M  AA++E KR+  A     ++S +T  ++  WI  Q+L  G +++FT  G
Sbjct: 435 VGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAG 494

Query: 508 LIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKD 567
           L+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT SS N  WL   ++N+ 
Sbjct: 495 LLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKSINRY 554

Query: 568 RLDLFYWLLAALSFLNFLNYLFWS 572
           +LD FYWL+  LS  NFL+YLFW+
Sbjct: 555 KLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of CmoCh04G009470 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 2.0e-107
Identity = 224/555 (40.36%), Postives = 333/555 (60.00%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N MH SL++S++ VT 
Sbjct: 13  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 72

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+ T FLLALLGGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 73  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS-SAA 132

Query: 148 DHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C    G KA   FV LYLV+LG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 133 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 192

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPI 267
             S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G+    I
Sbjct: 193 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 252

Query: 268 LQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIKSQDG 327
            +V +AA +       S+         S H+    S S     T     L+KA     +G
Sbjct: 253 FKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL----TNSLTCLNKAI----EG 312

Query: 328 TTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTKSF 387
            T    W  CT+ QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 313 KTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 372

Query: 388 HIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSA 447
           ++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 373 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 432

Query: 448 AIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 507
           A++E KR+  A     ++S +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 433 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 492

Query: 508 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALS 567
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 493 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 552

Query: 568 FLNFLNYLFWSSWFQ 576
            +NFL+YLFW+  ++
Sbjct: 553 VVNFLHYLFWAKRYK 555

BLAST of CmoCh04G009470 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 2.0e-107
Identity = 224/555 (40.36%), Postives = 333/555 (60.00%), Query Frame = 0

Query: 28  DWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNNLITYVINEMHFSLSKSANVVTN 87
           DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y+ N MH SL++S++ VT 
Sbjct: 17  DWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTT 76

Query: 88  FVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFILLSVQAHLPQLKPPQCNMLSEG 147
           F+ T FLLALLGGFL+D++  +F   LI   +E  G ILL++QA  P L PP C   S  
Sbjct: 77  FMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS-SAA 136

Query: 148 DHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGADQFTPQDPSQSKRLSTYFNAAYF 207
             C    G KA   FV LYLV+LG G +K ++ +HGA+QF    P   K+ ST+FN   F
Sbjct: 137 LRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVF 196

Query: 208 AFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGAIFIPI 267
             S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+NK P+G+    I
Sbjct: 197 CLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTI 256

Query: 268 LQVFVAAVLKRKQVCPSNPQMLHGSQTSNHKVALSSHSANLIPTQKFRFLDKACIKSQDG 327
            +V +AA +       S+         S H+    S S     T     L+KA     +G
Sbjct: 257 FKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKTPSQSL----TNSLTCLNKAI----EG 316

Query: 328 TTRESPWRLCTLTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTQLTKSF 387
            T    W  CT+ QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q + MN ++  +F
Sbjct: 317 KTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIV-NF 376

Query: 388 HIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSA 447
           ++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG GL LS  +M  A
Sbjct: 377 NVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVA 436

Query: 448 AIMEKKRRDSA-----VNSNQT--ISIFWITPQFLIFGLSEMFTAVGLIEFFYKQSVKGM 507
           A++E KR+  A     ++S +T  I+  WI  Q+L  G +++FT  GL+EFF+ ++   M
Sbjct: 437 ALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSM 496

Query: 508 QAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDNNLNKDRLDLFYWLLAALS 567
           ++  T++++ S + G+YLSS++V +VN++T S+    WL +  LN++RLDLFYWL+  LS
Sbjct: 497 RSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRLDLFYWLMCVLS 556

Query: 568 FLNFLNYLFWSSWFQ 576
            +NFL+YLFW+  ++
Sbjct: 557 VVNFLHYLFWAKRYK 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VV51.6e-23975.99Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ65.8e-22970.64Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8H1577.5e-11242.38Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE42.8e-10640.36Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9M3901.4e-10538.19Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FR740.0e+00100.00protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 ... [more]
A0A6J1JRW40.0e+0098.51protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE... [more]
A0A6J1EMI37.2e-29988.02protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JCZ74.7e-29888.15protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1C7K11.4e-29789.24protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146... [more]
Match NameE-valueIdentityDescription
XP_022942404.10.0e+00100.00protein NRT1/ PTR FAMILY 4.3-like [Cucurbita moschata][more]
KAG6600707.10.0e+0099.01Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022993122.10.0e+0098.51protein NRT1/ PTR FAMILY 4.3-like [Cucurbita maxima][more]
XP_023530877.10.0e+0098.01LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pe... [more]
XP_038903187.11.2e-30090.02protein NRT1/ PTR FAMILY 4.3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G59740.11.2e-24075.99Major facilitator superfamily protein [more]
AT1G33440.14.1e-23070.64Major facilitator superfamily protein [more]
AT1G69850.15.4e-11342.38nitrate transporter 1:2 [more]
AT1G27040.22.0e-10740.36Major facilitator superfamily protein [more]
AT1G27040.12.0e-10740.36Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 110..536
e-value: 2.6E-91
score: 306.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 22..586
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..579
e-value: 7.2E-163
score: 544.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 33..572
NoneNo IPR availablePANTHERPTHR11654:SF551PROTEIN NRT1/ PTR FAMILY 4.3coord: 22..586
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 42..573
e-value: 0.0
score: 651.012

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G009470.1CmoCh04G009470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity