CmoCh04G007600 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G007600
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionMembrane-associated kinase regulator 5
LocationCmo_Chr04: 3803920 .. 3806747 (+)
RNA-Seq ExpressionCmoCh04G007600
SyntenyCmoCh04G007600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGTTTGGAAAGGTTTTTGAATAATGCCTCTGCTTCTTCCTTCATTTACTCTGTCACTGTCTTTCTTATTGTGAACAGAACAGAGGAGGAGGACTGAGCCTTCACATGACTTCTCTGCAAATCACGCATTCAAATTCCACAACAAAACTAAATAAATAAATAAAGAAAGAAATTCTCCTCCTCCTCATCCTCCTCTGCAATACAACCATTACCATGTCCTTCTTCTGAACCTCTGACTCACCTCTCCGAACAACCTACTTTTTCTCTCCCTATCTTTACTTCTCTTTTCCTTTTTACCTCTCCTTTCTTCTTCTTCTTCTTCTCAATACCTTAATCCCTCTTCCTCATTTCTCTATTCTGGCTCTCCCAACCATGGAAAGCTTCAGCTTGCTTAAATACTGGAGAGGAGGCGGCGGTGGTGGTGGTGCGTCTGCTTCTGCCGCCGCTGATTTTGCTTTCAGCAGTACTTCATCTGCTGCCACCGCCACTGTTGTCACTGCCGTCGCGAGGAACCAGGCGGAGACTGATGAGGATGAGGATGACGATGATGGGCCGTTCTTTGATTTGGAGTTTGCGGTTCCTGATGAGGATGAGGGTCGGAAACAGGGGGGAGATGGAGATGGAGATGGAGATGGCAGAAACTGTGATGATGGAAGTGAGGAGGAGAATGATGATGATGAAGAAGAAGAAGAAGAAGAAGAAGATGGGGACTCGGATGGTGAGAGGGAGTTCGATTTCACGCTCTCGTCTGGCTCCACCAATGAACATTCCGATGCTAATCTTACGTTCTCTCCTTCAGATGACTTGTTTTTTAAGGGTAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAACCCAACTCCAAACCCCAGCTTCGTGTCACATTGTTGAAATCAGCCGCGAAGTTTAGGGTATTTATGTCGGGTCTCAAGAAATCGAAATCAAACGGGGAGAAGAAAACAGAGGTGGATGGATCTGTTGGGGAACCGACGAAAGGTGAGACAACAGAACTGACGCCGGAAAACCCACCGGAGAAAAACGAGAACCAATCGGACAAAAGCCAGAATCAAACGAAAAGTAAGTTATTTGCCGTGAAACTGAAGGTAGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGCTGGAACTACCACCACCACCACCACCACCAACAACAACAACAACAGTAACAAAGCACAGAAACAAAGCTCCGACGAATTAACCTCTGAAGAAAATCGATTTTCAAAAGACGTAATAATGCAAAAGTACTTAAAGATGGTGAAGCCTCTGTACATTCGGGTTTCACGGAGATACGGCGAGAAGTTCAGGTTATCCGGACAGCTGATTTTGGGGGCAGCCAGAGCTAAGTCCGGTGCACTGCCTACAATAGCGGCGCCACCCACTTCCCCACCGGTGGAAAAGACCGTGACAGACACAGAACTTGTGGAAGTACAAGCACAAACAACGACCATCAACACAAAGGGCCGGAACATGCCAGCCAAGCTAAGAGTGGTTTGCAAGCACTTGGGGAAAAGTCGGTCAGCTTCCTCCGCTGTAGCAGCGGCTCCACCCGGGCAGGCGCCGTCGAGAAGGGACGATTCACTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCATTGCAAGAGATCCTTCAACGCCTCTAGAGGTAAAAAAAAAAAAAAAAAAAAAACATAAACTGTTCTGTTACTTGTACTAAAAGCTTCACCTTTTTTCTTCAACTTCATTAATGGATCAATTCTTTTGTGTCTCTTCTACACCAAAAACAACAGATTCAGAGTCTTCCCTGCTGTCTCTGCGTGAGAAACCTTCAGATTTCACAGAGAAAGCCATTGGCTGAAGGTAAAGAAAAACAGCGATCGATCCATCAATCCATGAGAAAATTTCAACCCAAACAGGCGGGAAAATTTTGGTCAATATCCTTCACATTTCTCTCGCTTAATATATATATATATATATGTAGTATATATGTAAATGTTCATCTGTTGGGTTAGGAAGGATTGAATTTGTGGGTTCCTCTGGGCTTCTAAGTAAAAAGTTTGAATATTTTTCCAGTGAAGGGTAAAAAGTATTAGGAAGTGATGATACATAGAAAGCAAGCAGAGTTGGGCGCCCCATGTATGTGATTTTCCTATGCCTTCCCCAATTGAAATTTTAAGAATCACTGAGGTGAAGTGGGCGGTTTGAAATTATGTTGCTTTTTTTGGCTTTTTAGATTGGTAAAGGATGTGGGTCTCGGTAGGCCAGCCATCTCTGTGGCAAAAATAGTGTCTTTAGTGGTCAGAGAACTTGGGAAAAGTGGGGTTTTACTTAAACAGAGAAAATGGGTTCTATCTTTCCTTCCTTTTTCTTGTTTTTATCCAATGAATTATGGTCTAAAAGTAAGAGTAAAGGGGGGAAGCATGTGGGGGTTAGTTAAAATGACTTTATGGAGGTTTATTGAATGCCTGAATCAATTGAAGTTTTTTATGTTAATGTTTTAATATTAGGGGGGGTTTATGGATCCAATTTTGTGTTTTCAAGACACAGGGTTTTGATTTGGTCCCATGAAAATCACTGCCTCTGGGAATAATACTCTGTTGTTCTATGTCAAATTGTCATCATATTGTCCATATTATGGTTGGTTGTTTTCTTCAAATCATAGTCTTTTGTAAGTACATATAATCGTGTTCTAGTCTTATGGTTTGGAGCTTTCTGTGTTATGTTTTCTTCGCCTTTATTTTTTTAAACACCATTGTTGTCCATCACTCCATCCTCTTCTATGTTCTACTCTAT

mRNA sequence

AAAAGTTTGGAAAGGTTTTTGAATAATGCCTCTGCTTCTTCCTTCATTTACTCTGTCACTGTCTTTCTTATTGTGAACAGAACAGAGGAGGAGGACTGAGCCTTCACATGACTTCTCTGCAAATCACGCATTCAAATTCCACAACAAAACTAAATAAATAAATAAAGAAAGAAATTCTCCTCCTCCTCATCCTCCTCTGCAATACAACCATTACCATGTCCTTCTTCTGAACCTCTGACTCACCTCTCCGAACAACCTACTTTTTCTCTCCCTATCTTTACTTCTCTTTTCCTTTTTACCTCTCCTTTCTTCTTCTTCTTCTTCTCAATACCTTAATCCCTCTTCCTCATTTCTCTATTCTGGCTCTCCCAACCATGGAAAGCTTCAGCTTGCTTAAATACTGGAGAGGAGGCGGCGGTGGTGGTGGTGCGTCTGCTTCTGCCGCCGCTGATTTTGCTTTCAGCAGTACTTCATCTGCTGCCACCGCCACTGTTGTCACTGCCGTCGCGAGGAACCAGGCGGAGACTGATGAGGATGAGGATGACGATGATGGGCCGTTCTTTGATTTGGAGTTTGCGGTTCCTGATGAGGATGAGGGTCGGAAACAGGGGGGAGATGGAGATGGAGATGGAGATGGCAGAAACTGTGATGATGGAAGTGAGGAGGAGAATGATGATGATGAAGAAGAAGAAGAAGAAGAAGAAGATGGGGACTCGGATGGTGAGAGGGAGTTCGATTTCACGCTCTCGTCTGGCTCCACCAATGAACATTCCGATGCTAATCTTACGTTCTCTCCTTCAGATGACTTGTTTTTTAAGGGTAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAACCCAACTCCAAACCCCAGCTTCGTGTCACATTGTTGAAATCAGCCGCGAAGTTTAGGGTATTTATGTCGGGTCTCAAGAAATCGAAATCAAACGGGGAGAAGAAAACAGAGGTGGATGGATCTGTTGGGGAACCGACGAAAGGTGAGACAACAGAACTGACGCCGGAAAACCCACCGGAGAAAAACGAGAACCAATCGGACAAAAGCCAGAATCAAACGAAAAGTAAGTTATTTGCCGTGAAACTGAAGGTAGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGCTGGAACTACCACCACCACCACCACCACCAACAACAACAACAACAGTAACAAAGCACAGAAACAAAGCTCCGACGAATTAACCTCTGAAGAAAATCGATTTTCAAAAGACGTAATAATGCAAAAGTACTTAAAGATGGTGAAGCCTCTGTACATTCGGGTTTCACGGAGATACGGCGAGAAGTTCAGGTTATCCGGACAGCTGATTTTGGGGGCAGCCAGAGCTAAGTCCGGTGCACTGCCTACAATAGCGGCGCCACCCACTTCCCCACCGGTGGAAAAGACCGTGACAGACACAGAACTTGTGGAAGTACAAGCACAAACAACGACCATCAACACAAAGGGCCGGAACATGCCAGCCAAGCTAAGAGTGGTTTGCAAGCACTTGGGGAAAAGTCGGTCAGCTTCCTCCGCTGTAGCAGCGGCTCCACCCGGGCAGGCGCCGTCGAGAAGGGACGATTCACTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCATTGCAAGAGATCCTTCAACGCCTCTAGAGATTCAGAGTCTTCCCTGCTGTCTCTGCGTGAGAAACCTTCAGATTTCACAGAGAAAGCCATTGGCTGAAGGTAAAGAAAAACAGCGATCGATCCATCAATCCATGAGAAAATTTCAACCCAAACAGGCGGGAAAATTTTGGTCAATATCCTTCACATTTCTCTCGCTTAATATATATATATATATATGTAGTATATATGTAAATGTTCATCTGTTGGGTTAGGAAGGATTGAATTTGTGGGTTCCTCTGGGCTTCTAAGTAAAAAGTTTGAATATTTTTCCAGTGAAGGGTAAAAAGTATTAGGAAGTGATGATACATAGAAAGCAAGCAGAGTTGGGCGCCCCATGTATGTGATTTTCCTATGCCTTCCCCAATTGAAATTTTAAGAATCACTGAGGTGAAGTGGGCGGTTTGAAATTATGTTGCTTTTTTTGGCTTTTTAGATTGGTAAAGGATGTGGGTCTCGGTAGGCCAGCCATCTCTGTGGCAAAAATAGTGTCTTTAGTGGTCAGAGAACTTGGGAAAAGTGGGGTTTTACTTAAACAGAGAAAATGGGTTCTATCTTTCCTTCCTTTTTCTTGTTTTTATCCAATGAATTATGGTCTAAAAGTAAGAGTAAAGGGGGGAAGCATGTGGGGGTTAGTTAAAATGACTTTATGGAGGTTTATTGAATGCCTGAATCAATTGAAGTTTTTTATGTTAATGTTTTAATATTAGGGGGGGTTTATGGATCCAATTTTGTGTTTTCAAGACACAGGGTTTTGATTTGGTCCCATGAAAATCACTGCCTCTGGGAATAATACTCTGTTGTTCTATGTCAAATTGTCATCATATTGTCCATATTATGGTTGGTTGTTTTCTTCAAATCATAGTCTTTTGTAAGTACATATAATCGTGTTCTAGTCTTATGGTTTGGAGCTTTCTGTGTTATGTTTTCTTCGCCTTTATTTTTTTAAACACCATTGTTGTCCATCACTCCATCCTCTTCTATGTTCTACTCTAT

Coding sequence (CDS)

ATGGAAAGCTTCAGCTTGCTTAAATACTGGAGAGGAGGCGGCGGTGGTGGTGGTGCGTCTGCTTCTGCCGCCGCTGATTTTGCTTTCAGCAGTACTTCATCTGCTGCCACCGCCACTGTTGTCACTGCCGTCGCGAGGAACCAGGCGGAGACTGATGAGGATGAGGATGACGATGATGGGCCGTTCTTTGATTTGGAGTTTGCGGTTCCTGATGAGGATGAGGGTCGGAAACAGGGGGGAGATGGAGATGGAGATGGAGATGGCAGAAACTGTGATGATGGAAGTGAGGAGGAGAATGATGATGATGAAGAAGAAGAAGAAGAAGAAGAAGATGGGGACTCGGATGGTGAGAGGGAGTTCGATTTCACGCTCTCGTCTGGCTCCACCAATGAACATTCCGATGCTAATCTTACGTTCTCTCCTTCAGATGACTTGTTTTTTAAGGGTAAGCTTGTCCCTGTTGAAGCTTCCTCCAATGGCGGTTCTGAACCCAACTCCAAACCCCAGCTTCGTGTCACATTGTTGAAATCAGCCGCGAAGTTTAGGGTATTTATGTCGGGTCTCAAGAAATCGAAATCAAACGGGGAGAAGAAAACAGAGGTGGATGGATCTGTTGGGGAACCGACGAAAGGTGAGACAACAGAACTGACGCCGGAAAACCCACCGGAGAAAAACGAGAACCAATCGGACAAAAGCCAGAATCAAACGAAAAGTAAGTTATTTGCCGTGAAACTGAAGGTAGAGGAAGTTCCGATCATGTCTCTGTTTACCAGAGACAACAGTTCAAGAGGCTCCGCTGGAACTACCACCACCACCACCACCACCAACAACAACAACAACAGTAACAAAGCACAGAAACAAAGCTCCGACGAATTAACCTCTGAAGAAAATCGATTTTCAAAAGACGTAATAATGCAAAAGTACTTAAAGATGGTGAAGCCTCTGTACATTCGGGTTTCACGGAGATACGGCGAGAAGTTCAGGTTATCCGGACAGCTGATTTTGGGGGCAGCCAGAGCTAAGTCCGGTGCACTGCCTACAATAGCGGCGCCACCCACTTCCCCACCGGTGGAAAAGACCGTGACAGACACAGAACTTGTGGAAGTACAAGCACAAACAACGACCATCAACACAAAGGGCCGGAACATGCCAGCCAAGCTAAGAGTGGTTTGCAAGCACTTGGGGAAAAGTCGGTCAGCTTCCTCCGCTGTAGCAGCGGCTCCACCCGGGCAGGCGCCGTCGAGAAGGGACGATTCACTTCTGCAGCAACAAGACGGAATCCAGAGCGCCATTCTTCATTGCAAGAGATCCTTCAACGCCTCTAGAGATTCAGAGTCTTCCCTGCTGTCTCTGCGTGAGAAACCTTCAGATTTCACAGAGAAAGCCATTGGCTGA

Protein sequence

MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG
Homology
BLAST of CmoCh04G007600 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.7e-50
Identity = 186/467 (39.83%), Postives = 253/467 (54.18%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGG-----GGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDE 60
           ME+FSLL YW+  GG        + +S+++   +S+  S  T T+VT+V   + E + DE
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETEDE-EADE 60

Query: 61  DDDDGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSD 120
           +DD+GPFFDLEFA+P E+E    G   +GD D   C                      +D
Sbjct: 61  NDDEGPFFDLEFALPAEEEDTDGGVGREGDSD---C----------------------TD 120

Query: 121 GEREFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLV-----PVEASSNGGSEPNSKPQL 180
           G  EF FTLSS S  E     +     DD+FFK  +V     P  +     S      QL
Sbjct: 121 GGCEFKFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQL 180

Query: 181 RVTLLKSAAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSD 240
             ++LK+A K RVFM G+KKSK    K     GS          +L  +  P    + S 
Sbjct: 181 SASILKTATKLRVFMLGMKKSKLIQAKSEANFGS---------EDLDKQTHPPPPLSLSP 240

Query: 241 KSQNQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSD 300
           +SQ ++      V LKVEEVPI+SLF+R+NSS+ S     ++++++++++S+  +KQ+ +
Sbjct: 241 ESQQKS---TVTVNLKVEEVPIISLFSRENSSKNS-----SSSSSSSSSSSSSLKKQNGN 300

Query: 301 E-LTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIA 360
           E + S+E RF   V+MQKYLK VKPLYIRVSRRYGEK R SGQL                
Sbjct: 301 ESVVSDEKRF---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL---------------- 360

Query: 361 APPTSPPVEKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSAS------SA 420
              T+P   ++  +     V  +T        N+PA L+VV KHLGKSRS+S      S+
Sbjct: 361 ---TAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSS 399

Query: 421 VAAAPPGQAPS-RRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLL 450
             A    Q+ S RRDDSLLQQQD IQSAILHCKRSFN+SRD + S+L
Sbjct: 421 TTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDKDPSVL 399

BLAST of CmoCh04G007600 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 5.6e-06
Identity = 80/320 (25.00%), Postives = 130/320 (40.62%), Query Frame = 0

Query: 173 TLLKSAAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKS 232
           T +K   + R+  S +    + G +  ++D   GE +     EL       K   +  + 
Sbjct: 15  TTIKPRREIRISESAV--DSTTGSEDPDLDLCEGEDS---FFELKISLSDFKTPKEKQRL 74

Query: 233 QNQTKSKLFAVKLKVEEVPIMSLFTRDNSS-----------------RGSAGTTTTTTTT 292
           +  T +  ++V  K + +P + + ++   S                 +    TTT     
Sbjct: 75  ETTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQKFRAFSFKKSEKSTTTEKKKE 134

Query: 293 NNNNNSNKAQKQSSDELTS--------------EENRFSKDVIMQKYLKMVKPLYIRVSR 352
            NN  S   + +  DE T+               ++    D + +++  ++KPLY + ++
Sbjct: 135 ENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTK 194

Query: 353 RYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKTVTDTELVEVQAQTTTINTKGR 412
           +                        TI +P +SP                   T   +  
Sbjct: 195 KQSSS--------------------TITSPTSSP------------------ATREKQRS 254

Query: 413 NMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAILHCKRSFNAS 462
           N+P+ +R V + LGKSRSAS+A+    P    +R D+SL  QQDGIQSAILHCK+SF+ S
Sbjct: 255 NIPSGMRSVRRQLGKSRSASAAIGGMSPA---NRIDESLQVQQDGIQSAILHCKKSFHGS 286

BLAST of CmoCh04G007600 vs. ExPASy TrEMBL
Match: A0A6J1FPV4 (probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN=LOC111447389 PE=4 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.4e-238
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60
           MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120
           PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF
Sbjct: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120

Query: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180
           DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK
Sbjct: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240
           FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL
Sbjct: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300
           FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300

Query: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360
           KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT
Sbjct: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360

Query: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420
           VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL
Sbjct: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420

Query: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 465
           LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG
Sbjct: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 464

BLAST of CmoCh04G007600 vs. ExPASy TrEMBL
Match: A0A6J1J0G8 (probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=LOC111480174 PE=4 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 3.9e-220
Identity = 440/463 (95.03%), Postives = 443/463 (95.68%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60
           MESFSLLKYWR GGGGGGA ASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG
Sbjct: 1   MESFSLLKYWR-GGGGGGAPASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120
           PFFDLEFAVP+EDEGRKQG    GDGDGRNCDDGSEEENDD       EEDGDSDGEREF
Sbjct: 61  PFFDLEFAVPEEDEGRKQG----GDGDGRNCDDGSEEENDD-------EEDGDSDGEREF 120

Query: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180
           DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK
Sbjct: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240
           FRVFMSGLKKSKSNGEKKTE DGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL
Sbjct: 181 FRVFMSGLKKSKSNGEKKTETDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300
           FAVKLKVEEVPIMSLFTRDNSSRGSAG  TTTTT NNNNN+NKAQKQSSDELTSEENRFS
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAG--TTTTTNNNNNNNNKAQKQSSDELTSEENRFS 300

Query: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360
           KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSG LP+IAAPPTSPPVEK 
Sbjct: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGXLPSIAAPPTSPPVEKA 360

Query: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420
           VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL
Sbjct: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420

Query: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAI 464
           LQQQDGIQSAILHCKRSFNASRDSESSLLSLREK SDFTEKAI
Sbjct: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKASDFTEKAI 449

BLAST of CmoCh04G007600 vs. ExPASy TrEMBL
Match: A0A6J1JD60 (probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=LOC111483413 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 3.3e-179
Identity = 381/476 (80.04%), Postives = 412/476 (86.55%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGG--GGASASAAADFAFSSTSSAATA-TVVTAVARNQAETDEDEDD 60
           MESFSLLKYWRGGGG      +A+A ADFAF+  SSAA A +VVTAV +NQAE+DE+ D+
Sbjct: 1   MESFSLLKYWRGGGGATPPAVAAAATADFAFNGYSSAAGAVSVVTAVPQNQAESDEENDE 60

Query: 61  DDGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGE 120
           DDGPFFDLEFAVPDEDE RKQGG+ +G    RNC DGSEEE ++D+E++EEEEDGD+DGE
Sbjct: 61  DDGPFFDLEFAVPDEDESRKQGGNDEG---RRNCADGSEEEEEEDDEDDEEEEDGDTDGE 120

Query: 121 REFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKS 180
           REF+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSE N++PQLRVTLLKS
Sbjct: 121 REFNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSETNARPQLRVTLLKS 180

Query: 181 AAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTK 240
           AAKFRVFMSGLK+SKSNGEKK E DGSVGE TK E  E T E PPEKNENQ+ K QNQTK
Sbjct: 181 AAKFRVFMSGLKRSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQAGKEQNQTK 240

Query: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTT--TTTNNNNNSNKAQKQSSDELTSE 300
           SKLFAVKLKVEEVPIMSLFTRDNSSRGS GTT+ +     NNNNN+NK QK+SSDELTSE
Sbjct: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSNSNNNNNNNNNNNNKPQKRSSDELTSE 300

Query: 301 ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSP 360
           ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG     A PPTSP
Sbjct: 301 ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG-----APPPTSP 360

Query: 361 PVEKTVTD----TELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQ 420
           P+EK VT+    TE V+VQA TTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG 
Sbjct: 361 PLEKPVTETQTQTEGVQVQAPTTTANSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGM 420

Query: 421 APSRRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLLS------LREKPSDFTEK 462
           APSRRDDSL+QQQDGIQSAILHCKRSFNASRDSESSLLS      LREKPSD TEK
Sbjct: 421 APSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLLSRSVSDTLREKPSDLTEK 468

BLAST of CmoCh04G007600 vs. ExPASy TrEMBL
Match: A0A6J1EU24 (probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN=LOC111437571 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 3.2e-174
Identity = 371/458 (81.00%), Postives = 399/458 (87.12%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGA-SASAAADFAFSSTSSAATA-TVVTAVARNQAETDEDEDDD 60
           MESFSLLKYWRGGGG   A +A+A+ADFAF+  SSAA A +VVTAV +NQAE+DE+ D+D
Sbjct: 1   MESFSLLKYWRGGGGATSAVAAAASADFAFNGNSSAAGAVSVVTAVPQNQAESDEENDED 60

Query: 61  DGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGER 120
           DGPFFDLEFAVPDEDE RKQGG+ +G    RNC DGSEEE +DD  +++EEEDGDSDGER
Sbjct: 61  DGPFFDLEFAVPDEDESRKQGGNDEG---RRNCADGSEEEEEDD--DDDEEEDGDSDGER 120

Query: 121 EFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSA 180
           EF+FTLSSGS+NEHSDANL FSPSDDLFFKGKLVPVEASSNGGSEPN +PQLRVTLLKSA
Sbjct: 121 EFNFTLSSGSSNEHSDANLAFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSA 180

Query: 181 AKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKS 240
           AKFRVFMSGLKKSKSNGEKK E DGSVGE TK E  E T E PPEKNENQ  K Q+QTKS
Sbjct: 181 AKFRVFMSGLKKSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQPVKEQSQTKS 240

Query: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENR 300
           KLFAVKLKVEEVPIMSLFTRDNSSRGS G    TT++NNNNN+NK QKQSSDELTSEENR
Sbjct: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSGG----TTSSNNNNNNNKPQKQSSDELTSEENR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVE 360
           FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG     + PPTSPP+E
Sbjct: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG-----SPPPTSPPLE 360

Query: 361 KTVTDTEL------VEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQA 420
           K VT+TE       V+VQA TTT N+KGR MPAKLRVVCKHLGKSRSASSAVAAAPPG A
Sbjct: 361 KPVTETETQTQTEGVQVQAPTTTANSKGRTMPAKLRVVCKHLGKSRSASSAVAAAPPGMA 420

Query: 421 PSRRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLLS 451
           PSRRDDSL+QQQDGIQSAILHCKRSFNASRDSESSLLS
Sbjct: 421 PSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLLS 444

BLAST of CmoCh04G007600 vs. ExPASy TrEMBL
Match: A0A5A7TMX4 (Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001450 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 4.2e-174
Identity = 370/472 (78.39%), Postives = 399/472 (84.53%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSA-ATATVVTAVARNQAETDEDE-DDD 60
           MESFSLLKYWRGGG G G  A+AAADFAF+S SSA ATATVVTAV R + ETDE+E D+D
Sbjct: 1   MESFSLLKYWRGGGAGAG--ATAAADFAFNSNSSASATATVVTAVPRKEVETDEEENDND 60

Query: 61  DGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGER 120
           DGPFFDLEFAVPDEDEGRKQG D         C DGSEEE ++D++++E++EDGD D ER
Sbjct: 61  DGPFFDLEFAVPDEDEGRKQGTD---------CIDGSEEEGEEDDDDDEDDEDGDGD-ER 120

Query: 121 EFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSA 180
           EF+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPN +PQLRVTLLKSA
Sbjct: 121 EFNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNVRPQLRVTLLKSA 180

Query: 181 AKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKS 240
           AKFRVFMSGLKKSKSNGEKKTE+DGSVGE  K ET E T  NP EKNENQ +K QNQTK+
Sbjct: 181 AKFRVFMSGLKKSKSNGEKKTEMDGSVGETAKSETKEETTGNPSEKNENQLEKDQNQTKN 240

Query: 241 KLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENR 300
           K FAVK KVEEVPIMSLFTRDNSSRGS  TT           +NK QKQ+SDE T EE R
Sbjct: 241 KFFAVKFKVEEVPIMSLFTRDNSSRGSGVTT-----------NNKPQKQTSDESTPEETR 300

Query: 301 FSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVE 360
           FSKDV+MQKYLKMVKPLYIRVSRRYGEKF+LSGQLILGAA AKS A P+IAAPPTSP  E
Sbjct: 301 FSKDVLMQKYLKMVKPLYIRVSRRYGEKFKLSGQLILGAAGAKSSAPPSIAAPPTSPSTE 360

Query: 361 KTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDD 420
           K+V +TE V+V+  TTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG APSRRDD
Sbjct: 361 KSVVETEAVQVKPPTTTTNSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGVAPSRRDD 420

Query: 421 SLLQQQDGIQSAILHCKRSFNASRDSESSLLS------LREKPSDFTEKAIG 465
           SLLQQQDGIQSAILHCKRSFNASR SESSLLS      LREKPSD TEKAIG
Sbjct: 421 SLLQQQDGIQSAILHCKRSFNASRGSESSLLSRSVSDTLREKPSDLTEKAIG 449

BLAST of CmoCh04G007600 vs. NCBI nr
Match: XP_022942302.1 (probable membrane-associated kinase regulator 2 [Cucurbita moschata])

HSP 1 Score: 834.3 bits (2154), Expect = 5.0e-238
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60
           MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120
           PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF
Sbjct: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120

Query: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180
           DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK
Sbjct: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240
           FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL
Sbjct: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300
           FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300

Query: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360
           KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT
Sbjct: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360

Query: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420
           VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL
Sbjct: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420

Query: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 465
           LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG
Sbjct: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 464

BLAST of CmoCh04G007600 vs. NCBI nr
Match: KAG6600544.1 (putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031183.1 putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805.4 bits (2079), Expect = 2.5e-229
Identity = 458/467 (98.07%), Postives = 458/467 (98.07%), Query Frame = 0

Query: 1   MESFSLLKYWR-GGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDD 60
           MESFSLLKYWR GGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDD
Sbjct: 1   MESFSLLKYWRGGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDD 60

Query: 61  GPFFDLEFAVPDEDEGRKQG--GDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGE 120
           GPFFDLEFAVPDEDEGRKQG  GDGDGDGDGRNCDDGSEEENDDD  EEEEEEDGDSDGE
Sbjct: 61  GPFFDLEFAVPDEDEGRKQGGDGDGDGDGDGRNCDDGSEEENDDD--EEEEEEDGDSDGE 120

Query: 121 REFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKS 180
           REFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKS
Sbjct: 121 REFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKS 180

Query: 181 AAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTK 240
           AAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTK ETTELTPENPPEKNENQSDKSQNQTK
Sbjct: 181 AAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKSETTELTPENPPEKNENQSDKSQNQTK 240

Query: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEEN 300
           SKLFAVKLKVEEVPIMSLFTRDNSSRGSAG   TTTTTNNNNNSNKAQKQSSDELTSEEN
Sbjct: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSAG---TTTTTNNNNNSNKAQKQSSDELTSEEN 300

Query: 301 RFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPV 360
           RFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPV
Sbjct: 301 RFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPV 360

Query: 361 EKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRD 420
           EKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRD
Sbjct: 361 EKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRD 420

Query: 421 DSLLQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 465
           DSLLQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG
Sbjct: 421 DSLLQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 462

BLAST of CmoCh04G007600 vs. NCBI nr
Match: XP_023529752.1 (probable membrane-associated kinase regulator 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.8 bits (2041), Expect = 6.4e-225
Identity = 447/464 (96.34%), Postives = 452/464 (97.41%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60
           MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG
Sbjct: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120
           PFFDLEFAVPDEDEGRKQG    GDGD RNCDDGSEEEND+D EEEEEEEDGDSDGEREF
Sbjct: 61  PFFDLEFAVPDEDEGRKQG----GDGDDRNCDDGSEEENDED-EEEEEEEDGDSDGEREF 120

Query: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180
           DFTLSSGSTNEHSDANLTFSPSDDLFFKG+L+PVEASSNGGSEPNSKPQLRVTLLKSAAK
Sbjct: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGRLLPVEASSNGGSEPNSKPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240
           FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL
Sbjct: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300
           FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTT    NN+NKAQKQSSDELTSEE RFS
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTT----NNNNKAQKQSSDELTSEETRFS 300

Query: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360
           KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALP+IAAPPTSPPVEK 
Sbjct: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPSIAAPPTSPPVEKA 360

Query: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420
           VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL
Sbjct: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420

Query: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 465
           LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG
Sbjct: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAIG 455

BLAST of CmoCh04G007600 vs. NCBI nr
Match: XP_022980889.1 (probable membrane-associated kinase regulator 2 [Cucurbita maxima])

HSP 1 Score: 773.9 bits (1997), Expect = 8.0e-220
Identity = 440/463 (95.03%), Postives = 443/463 (95.68%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGGGGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60
           MESFSLLKYWR GGGGGGA ASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG
Sbjct: 1   MESFSLLKYWR-GGGGGGAPASAAADFAFSSTSSAATATVVTAVARNQAETDEDEDDDDG 60

Query: 61  PFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGEREF 120
           PFFDLEFAVP+EDEGRKQG    GDGDGRNCDDGSEEENDD       EEDGDSDGEREF
Sbjct: 61  PFFDLEFAVPEEDEGRKQG----GDGDGRNCDDGSEEENDD-------EEDGDSDGEREF 120

Query: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180
           DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK
Sbjct: 121 DFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKSAAK 180

Query: 181 FRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240
           FRVFMSGLKKSKSNGEKKTE DGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL
Sbjct: 181 FRVFMSGLKKSKSNGEKKTETDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTKSKL 240

Query: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSDELTSEENRFS 300
           FAVKLKVEEVPIMSLFTRDNSSRGSAG  TTTTT NNNNN+NKAQKQSSDELTSEENRFS
Sbjct: 241 FAVKLKVEEVPIMSLFTRDNSSRGSAG--TTTTTNNNNNNNNKAQKQSSDELTSEENRFS 300

Query: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKT 360
           KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSG LP+IAAPPTSPPVEK 
Sbjct: 301 KDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGXLPSIAAPPTSPPVEKA 360

Query: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420
           VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL
Sbjct: 361 VTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSL 420

Query: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKPSDFTEKAI 464
           LQQQDGIQSAILHCKRSFNASRDSESSLLSLREK SDFTEKAI
Sbjct: 421 LQQQDGIQSAILHCKRSFNASRDSESSLLSLREKASDFTEKAI 449

BLAST of CmoCh04G007600 vs. NCBI nr
Match: XP_022985394.1 (probable membrane-associated kinase regulator 2 [Cucurbita maxima])

HSP 1 Score: 637.9 bits (1644), Expect = 6.9e-179
Identity = 381/476 (80.04%), Postives = 412/476 (86.55%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGG--GGASASAAADFAFSSTSSAATA-TVVTAVARNQAETDEDEDD 60
           MESFSLLKYWRGGGG      +A+A ADFAF+  SSAA A +VVTAV +NQAE+DE+ D+
Sbjct: 1   MESFSLLKYWRGGGGATPPAVAAAATADFAFNGYSSAAGAVSVVTAVPQNQAESDEENDE 60

Query: 61  DDGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSDGE 120
           DDGPFFDLEFAVPDEDE RKQGG+ +G    RNC DGSEEE ++D+E++EEEEDGD+DGE
Sbjct: 61  DDGPFFDLEFAVPDEDESRKQGGNDEG---RRNCADGSEEEEEEDDEDDEEEEDGDTDGE 120

Query: 121 REFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSEPNSKPQLRVTLLKS 180
           REF+FTLSSGS+NEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSE N++PQLRVTLLKS
Sbjct: 121 REFNFTLSSGSSNEHSDANLTFSPSDDLFFKGKLVPVEASSNGGSETNARPQLRVTLLKS 180

Query: 181 AAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKSQNQTK 240
           AAKFRVFMSGLK+SKSNGEKK E DGSVGE TK E  E T E PPEKNENQ+ K QNQTK
Sbjct: 181 AAKFRVFMSGLKRSKSNGEKKPETDGSVGEMTKTEMKEETTEIPPEKNENQAGKEQNQTK 240

Query: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTT--TTTNNNNNSNKAQKQSSDELTSE 300
           SKLFAVKLKVEEVPIMSLFTRDNSSRGS GTT+ +     NNNNN+NK QK+SSDELTSE
Sbjct: 241 SKLFAVKLKVEEVPIMSLFTRDNSSRGSGGTTSNSNNNNNNNNNNNNKPQKRSSDELTSE 300

Query: 301 ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIAAPPTSP 360
           ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAA AKSG     A PPTSP
Sbjct: 301 ENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAAGAKSG-----APPPTSP 360

Query: 361 PVEKTVTD----TELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGQ 420
           P+EK VT+    TE V+VQA TTT N+KGRNMPAKLRVVCKHLGKSRSASSAVAAAPPG 
Sbjct: 361 PLEKPVTETQTQTEGVQVQAPTTTANSKGRNMPAKLRVVCKHLGKSRSASSAVAAAPPGM 420

Query: 421 APSRRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLLS------LREKPSDFTEK 462
           APSRRDDSL+QQQDGIQSAILHCKRSFNASRDSESSLLS      LREKPSD TEK
Sbjct: 421 APSRRDDSLIQQQDGIQSAILHCKRSFNASRDSESSLLSRSVSDTLREKPSDLTEK 468

BLAST of CmoCh04G007600 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-51
Identity = 186/467 (39.83%), Postives = 253/467 (54.18%), Query Frame = 0

Query: 1   MESFSLLKYWRGGGGG-----GGASASAAADFAFSSTSSAATATVVTAVARNQAETDEDE 60
           ME+FSLL YW+  GG        + +S+++   +S+  S  T T+VT+V   + E + DE
Sbjct: 1   MEAFSLLNYWKNSGGAVTFLPHSSESSSSSLCRYSAGESIGTTTIVTSVTETEDE-EADE 60

Query: 61  DDDDGPFFDLEFAVPDEDEGRKQGGDGDGDGDGRNCDDGSEEENDDDEEEEEEEEDGDSD 120
           +DD+GPFFDLEFA+P E+E    G   +GD D   C                      +D
Sbjct: 61  NDDEGPFFDLEFALPAEEEDTDGGVGREGDSD---C----------------------TD 120

Query: 121 GEREFDFTLSSGSTNEHSDANLTFSPSDDLFFKGKLV-----PVEASSNGGSEPNSKPQL 180
           G  EF FTLSS S  E     +     DD+FFK  +V     P  +     S      QL
Sbjct: 121 GGCEFKFTLSSCSGGED---RVDPDSLDDVFFKETIVEEVEPPTSSEQQTCSVKAPAAQL 180

Query: 181 RVTLLKSAAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSD 240
             ++LK+A K RVFM G+KKSK    K     GS          +L  +  P    + S 
Sbjct: 181 SASILKTATKLRVFMLGMKKSKLIQAKSEANFGS---------EDLDKQTHPPPPLSLSP 240

Query: 241 KSQNQTKSKLFAVKLKVEEVPIMSLFTRDNSSRGSAGTTTTTTTTNNNNNSNKAQKQSSD 300
           +SQ ++      V LKVEEVPI+SLF+R+NSS+ S     ++++++++++S+  +KQ+ +
Sbjct: 241 ESQQKS---TVTVNLKVEEVPIISLFSRENSSKNS-----SSSSSSSSSSSSSLKKQNGN 300

Query: 301 E-LTSEENRFSKDVIMQKYLKMVKPLYIRVSRRYGEKFRLSGQLILGAARAKSGALPTIA 360
           E + S+E RF   V+MQKYLK VKPLYIRVSRRYGEK R SGQL                
Sbjct: 301 ESVVSDEKRF---VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQL---------------- 360

Query: 361 APPTSPPVEKTVTDTELVEVQAQTTTINTKGRNMPAKLRVVCKHLGKSRSAS------SA 420
              T+P   ++  +     V  +T        N+PA L+VV KHLGKSRS+S      S+
Sbjct: 361 ---TAPSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSS 399

Query: 421 VAAAPPGQAPS-RRDDSLLQQQDGIQSAILHCKRSFNASRDSESSLL 450
             A    Q+ S RRDDSLLQQQD IQSAILHCKRSFN+SRD + S+L
Sbjct: 421 TTATVTTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDKDPSVL 399

BLAST of CmoCh04G007600 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-07
Identity = 80/320 (25.00%), Postives = 130/320 (40.62%), Query Frame = 0

Query: 173 TLLKSAAKFRVFMSGLKKSKSNGEKKTEVDGSVGEPTKGETTELTPENPPEKNENQSDKS 232
           T +K   + R+  S +    + G +  ++D   GE +     EL       K   +  + 
Sbjct: 15  TTIKPRREIRISESAV--DSTTGSEDPDLDLCEGEDS---FFELKISLSDFKTPKEKQRL 74

Query: 233 QNQTKSKLFAVKLKVEEVPIMSLFTRDNSS-----------------RGSAGTTTTTTTT 292
           +  T +  ++V  K + +P + + ++   S                 +    TTT     
Sbjct: 75  ETTTTTTTYSVSNKSKVLPFVDISSKPQQSPTTLLKSGQKFRAFSFKKSEKSTTTEKKKE 134

Query: 293 NNNNNSNKAQKQSSDELTS--------------EENRFSKDVIMQKYLKMVKPLYIRVSR 352
            NN  S   + +  DE T+               ++    D + +++  ++KPLY + ++
Sbjct: 135 ENNRTSLNVRFRVDDETTTTSFRKTASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTK 194

Query: 353 RYGEKFRLSGQLILGAARAKSGALPTIAAPPTSPPVEKTVTDTELVEVQAQTTTINTKGR 412
           +                        TI +P +SP                   T   +  
Sbjct: 195 KQSSS--------------------TITSPTSSP------------------ATREKQRS 254

Query: 413 NMPAKLRVVCKHLGKSRSASSAVAAAPPGQAPSRRDDSLLQQQDGIQSAILHCKRSFNAS 462
           N+P+ +R V + LGKSRSAS+A+    P    +R D+SL  QQDGIQSAILHCK+SF+ S
Sbjct: 255 NIPSGMRSVRRQLGKSRSASAAIGGMSPA---NRIDESLQVQQDGIQSAILHCKKSFHGS 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SH581.7e-5039.83Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9FLX45.6e-0625.00Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FPV42.4e-238100.00probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J0G83.9e-22095.03probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JD603.3e-17980.04probable membrane-associated kinase regulator 2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EU243.2e-17481.00probable membrane-associated kinase regulator 2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7TMX44.2e-17478.39Putative membrane-associated kinase regulator 2 OS=Cucumis melo var. makuwa OX=1... [more]
Match NameE-valueIdentityDescription
XP_022942302.15.0e-238100.00probable membrane-associated kinase regulator 2 [Cucurbita moschata][more]
KAG6600544.12.5e-22998.07putative membrane-associated kinase regulator 2, partial [Cucurbita argyrosperma... [more]
XP_023529752.16.4e-22596.34probable membrane-associated kinase regulator 2 [Cucurbita pepo subsp. pepo][more]
XP_022980889.18.0e-22095.03probable membrane-associated kinase regulator 2 [Cucurbita maxima][more]
XP_022985394.16.9e-17980.04probable membrane-associated kinase regulator 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G64080.11.2e-5139.83unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT5G52870.14.0e-0725.00unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..205
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 439..464
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..288
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..420
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 186..236
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 447..464
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 121..140
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 46..144
NoneNo IPR availablePANTHERPTHR33929:SF1MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..451
IPR039619Membrane-associated kinase regulator 2/5PANTHERPTHR33929MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATEDcoord: 1..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G007600.1CmoCh04G007600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity