CmoCh04G005210 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G005210
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPI-PLC X domain-containing protein At5g67130-like
LocationCmo_Chr04: 2588248 .. 2590106 (-)
RNA-Seq ExpressionCmoCh04G005210
SyntenyCmoCh04G005210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GACAGTGAGTGGAGTAAATACCTGTAAGCCTCTGCGCGCCATATTGAGTCCATTACATCGCAGCCTCAGCGTCCTCTGTTTACAGGAACTCGCCTTCCATCGCTCCAAACACCGCCCCCTCGCTCGCCTCCACCAAATGCCTCGTCCTGGTTTCTCCCGGAGATTACTTCTGGCGGCGACTCTGTTTCCCATTATCTTCCTCTTCCACTCTTCTTCAGCTCGCAAGGTTCGAACACCCATTTCGTTTCTTTTCCTTTTTTCGAGACTCTGGGATTTTCAGCTGCAATTGTTTGGGTTCCTCTGTTTTTGCAGGAGGGTGGGACGTGTCTTTTTAATAGTAACTGCGACGCCGGCTTGCATTGCCAGACTTGCATTGCCAACGGCAATATCCGGCCGCGTTGCACTCGAATCCAGCCGATCAGTCCAATTTCAAAGGTCTGTGTTAAAAATGAGAATCTCTGAATACTATGTTTCAAGGACTTTTATCCAACTCATGTTTCTCATTCTTAACACGATATCAAAGCTTATCACAGCTTTTCATTCTTAACCGTATGTAGCTATAATGATTCGGGTTATTTTTGAACTTTTGGAGCAGGTGAATGGTCTTCCATTTAATAAATACACATGGTTGACGACCCACAATTCATTCGCCATATTAGGGGCGAAGTCTGAAACTGGGACTCCGATTCTTGCTCCTACCAATCAACAGGGCTCCATTACTAGCCAGCTCAATGTACGTTACTGCCTTTCCGTTTCTGCTTATTGATTATTCATAACTTCTAAGCCTCTGTACTACGAAACCTTTTCACTTTGCAGAATGGAGTCAGAGGATTTATGCTTGATACGTATGACTTTCGAAATGATATATGGTTATGTCACTCATTCGAAGGCAAATGCCACGACTTTACAGCTTTCGTAAGTGTCCAATTTGTAGACTGCCATTCTGTGTTCTGAATGTTGCCTCAATATGCCATTATTTCTCCTTGTTTATGATATCTTGAACAAGTTCATGCAAAACTTTGAATGCCCAGCAACCAGCCATTGAGGTTCTGAAAGAGTTCCAAGCATTTCTTGGAGCAAACCCTTCTGAAATCGTCACCATTATCATCGAAGACTATGTCACGGCACCAAACGGACTAACTAATGTGTTTGCTGCTGCTGGTCTGAAACAATTCTGGTTCCCAGTATCACGTATGCCTAAGAATGGTGGAGATTGGCCCACTGTTAGTGACATAATCCAGAAAAACCAACGCTTGCTTGTTTTTACTTCCAAAGCGGCAAAAGAAGCCTCTGAAGGAATTGCATACGTATGGAATTACATGGTAGAAAACCAATGTAAGGTCATTTCTTATTTCACCACACTGAATAAAGCTTCCTGAATCCAAAATCCAATAGTTTTTCTTATAGATTCAATCCTTATACAGATGGCGATGAAGGAATGAAAGCAGGTTCATGCCCAAACCGAGGAGAATCAACACCGATGAACACGACTTCAAGGTCACTCGTAATCGTAAATTTCTTCAGAAGTGCTTCAAATTTCCCTGCGGCTTGCAAGGACAACTCAGCTCCATTACTCAACATGGTGAACACTTGCTATAATGCAGCAGGAAAAAGATGGCCTAACTTCATTGCTGTTGACTTCTACAAGGTAATCAAGCACAAAACTAAGCCAACAAAATCAGTTGCTTCAAATTCCTTTGAAACTTATCAATGATATAGACTAAACATAGTTCAATTTGGAATGTATATCACAGAGAAGTGATGGGGGAGGAGCTCCAGAAGCCGTTGATGTATCGAACGGTCAGTCTATTTGTGGATGTCCAACCATTTCCAATTGCAAGGTTAGCCATTACTACTGA

mRNA sequence

GACAGTGAGTGGAGTAAATACCTGTAAGCCTCTGCGCGCCATATTGAGTCCATTACATCGCAGCCTCAGCGTCCTCTGTTTACAGGAACTCGCCTTCCATCGCTCCAAACACCGCCCCCTCGCTCGCCTCCACCAAATGCCTCGTCCTGGTTTCTCCCGGAGATTACTTCTGGCGGCGACTCTGTTTCCCATTATCTTCCTCTTCCACTCTTCTTCAGCTCGCAAGGAGGGTGGGACGTGTCTTTTTAATAGTAACTGCGACGCCGGCTTGCATTGCCAGACTTGCATTGCCAACGGCAATATCCGGCCGCGTTGCACTCGAATCCAGCCGATCAGTCCAATTTCAAAGGTGAATGGTCTTCCATTTAATAAATACACATGGTTGACGACCCACAATTCATTCGCCATATTAGGGGCGAAGTCTGAAACTGGGACTCCGATTCTTGCTCCTACCAATCAACAGGGCTCCATTACTAGCCAGCTCAATAATGGAGTCAGAGGATTTATGCTTGATACGTATGACTTTCGAAATGATATATGGTTATGTCACTCATTCGAAGGCAAATGCCACGACTTTACAGCTTTCCAACCAGCCATTGAGGTTCTGAAAGAGTTCCAAGCATTTCTTGGAGCAAACCCTTCTGAAATCGTCACCATTATCATCGAAGACTATGTCACGGCACCAAACGGACTAACTAATGTGTTTGCTGCTGCTGGTCTGAAACAATTCTGGTTCCCAGTATCACGTATGCCTAAGAATGGTGGAGATTGGCCCACTGTTAGTGACATAATCCAGAAAAACCAACGCTTGCTTGTTTTTACTTCCAAAGCGGCAAAAGAAGCCTCTGAAGGAATTGCATACGTATGGAATTACATGGTAGAAAACCAATATGGCGATGAAGGAATGAAAGCAGGTTCATGCCCAAACCGAGGAGAATCAACACCGATGAACACGACTTCAAGGTCACTCGTAATCGTAAATTTCTTCAGAAGTGCTTCAAATTTCCCTGCGGCTTGCAAGGACAACTCAGCTCCATTACTCAACATGGTGAACACTTGCTATAATGCAGCAGGAAAAAGATGGCCTAACTTCATTGCTGTTGACTTCTACAAGAGAAGTGATGGGGGAGGAGCTCCAGAAGCCGTTGATGTATCGAACGGTCAGTCTATTTGTGGATGTCCAACCATTTCCAATTGCAAGGTTAGCCATTACTACTGA

Coding sequence (CDS)

ATGCCTCGTCCTGGTTTCTCCCGGAGATTACTTCTGGCGGCGACTCTGTTTCCCATTATCTTCCTCTTCCACTCTTCTTCAGCTCGCAAGGAGGGTGGGACGTGTCTTTTTAATAGTAACTGCGACGCCGGCTTGCATTGCCAGACTTGCATTGCCAACGGCAATATCCGGCCGCGTTGCACTCGAATCCAGCCGATCAGTCCAATTTCAAAGGTGAATGGTCTTCCATTTAATAAATACACATGGTTGACGACCCACAATTCATTCGCCATATTAGGGGCGAAGTCTGAAACTGGGACTCCGATTCTTGCTCCTACCAATCAACAGGGCTCCATTACTAGCCAGCTCAATAATGGAGTCAGAGGATTTATGCTTGATACGTATGACTTTCGAAATGATATATGGTTATGTCACTCATTCGAAGGCAAATGCCACGACTTTACAGCTTTCCAACCAGCCATTGAGGTTCTGAAAGAGTTCCAAGCATTTCTTGGAGCAAACCCTTCTGAAATCGTCACCATTATCATCGAAGACTATGTCACGGCACCAAACGGACTAACTAATGTGTTTGCTGCTGCTGGTCTGAAACAATTCTGGTTCCCAGTATCACGTATGCCTAAGAATGGTGGAGATTGGCCCACTGTTAGTGACATAATCCAGAAAAACCAACGCTTGCTTGTTTTTACTTCCAAAGCGGCAAAAGAAGCCTCTGAAGGAATTGCATACGTATGGAATTACATGGTAGAAAACCAATATGGCGATGAAGGAATGAAAGCAGGTTCATGCCCAAACCGAGGAGAATCAACACCGATGAACACGACTTCAAGGTCACTCGTAATCGTAAATTTCTTCAGAAGTGCTTCAAATTTCCCTGCGGCTTGCAAGGACAACTCAGCTCCATTACTCAACATGGTGAACACTTGCTATAATGCAGCAGGAAAAAGATGGCCTAACTTCATTGCTGTTGACTTCTACAAGAGAAGTGATGGGGGAGGAGCTCCAGAAGCCGTTGATGTATCGAACGGTCAGTCTATTTGTGGATGTCCAACCATTTCCAATTGCAAGGTTAGCCATTACTACTGA

Protein sequence

MPRPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY
Homology
BLAST of CmoCh04G005210 vs. ExPASy Swiss-Prot
Match: Q93XX5 (PI-PLC X domain-containing protein At5g67130 OS=Arabidopsis thaliana OX=3702 GN=At5g67130 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 5.0e-111
Identity = 193/354 (54.52%), Postives = 244/354 (68.93%), Query Frame = 0

Query: 12  LAATLFPIIFLFHSSSARKEGG-----TCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPI 71
           +  +L  ++  F  SSA   G      +C   ++C +GL+C  C A G  +P CTR Q  
Sbjct: 12  VTVSLLLLLLSFSFSSACSNGNCQLLDSCSSATDCVSGLYCGDCPAVGRSKPVCTRGQAT 71

Query: 72  SPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLD 131
           SP S +NGLPFNKYTWL THN+F+   A    G   +   NQ+ +IT+QL NGVRG MLD
Sbjct: 72  SPTSIINGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQNGVRGLMLD 131

Query: 132 TYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGL 191
            YDF NDIWLCHS  G+C +FTAFQPAI +L+E +AFL  NP+EIVTIIIEDYV  P GL
Sbjct: 132 MYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIEDYVHRPKGL 191

Query: 192 TNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNY 251
           + +FA AGL ++WFPVS+MP+ G DWPTV+D++Q+N RLLVFTS AAKE  EG+AY W Y
Sbjct: 192 STLFANAGLDKYWFPVSKMPRKGEDWPTVTDMVQENHRLLVFTSVAAKEDEEGVAYQWRY 251

Query: 252 MVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVN 311
           MVEN+ GD G+K GSCPNR ES P+N+ S SL ++N+F +      ACK++SAPL  MV 
Sbjct: 252 MVENESGDPGVKRGSCPNRKESQPLNSKSSSLFLMNYFPTYPVEKDACKEHSAPLAEMVG 311

Query: 312 TCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 361
           TC  + G R PNF+AV+FY RSDGGG  E +D  NG  +CGC T+S C+    Y
Sbjct: 312 TCLKSGGNRMPNFLAVNFYMRSDGGGVFEILDRMNGPVLCGCETLSACQPGAAY 365

BLAST of CmoCh04G005210 vs. ExPASy Swiss-Prot
Match: D4AUX6 (Uncharacterized secreted protein ARB_08043 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_08043 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 9.3e-09
Identity = 52/202 (25.74%), Postives = 86/202 (42.57%), Query Frame = 0

Query: 58  PRCTRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAP----TNQQGSIT 117
           P+ T  +P +  ++     +   T +  HNS            P + P    +NQ+  + 
Sbjct: 109 PQPTNTRPCNGYAEFCARSYGNITQVAAHNS------------PFVRPGNIASNQELDVV 168

Query: 118 SQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVT 177
           +QLN+G+R     T+     I+LCHS    C D          LK+   +L  NP ++V+
Sbjct: 169 TQLNDGIRMLQFQTHLVNGTIYLCHS---SC-DLLNAGTLESYLKKVADWLRDNPYDVVS 228

Query: 178 III--EDYVTAPNGLTNVFAAAGLKQFWFPVSRMPKNGG----DWPTVSDIIQKNQRLLV 237
           ++I   D+V   N  T    ++GL    +     PKN      DWPT+S++I   +R +V
Sbjct: 229 LLIGNGDFVGVKN-FTAPIQSSGLIDHVY----TPKNHSIALDDWPTLSEVILSGKRAMV 288

Query: 238 FTSKAAKEAS-----EGIAYVW 245
           F    A         +   Y+W
Sbjct: 289 FMDYEANHGEVPYILDEFTYIW 289

BLAST of CmoCh04G005210 vs. ExPASy Swiss-Prot
Match: D4AK17 (PI-PLC X domain-containing protein 1 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_04618 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.0e-07
Identity = 64/267 (23.97%), Postives = 116/267 (43.45%), Query Frame = 0

Query: 86  HNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCH 145
           ++  + +GA +      L   NQ  S+T QL+ G+R     T+  +ND          CH
Sbjct: 44  YSELSFVGAHNSPFVGPLLQHNQDISVTEQLDFGIRFLQGQTH--KND----DGVFSMCH 103

Query: 146 DFTAFQPAIEV---LKEFQAFLGANPSEIVTIIIEDYVTAPNGL-----TNVF-AAAGLK 205
                + A  V   L+  + +L ++P+E+VT++I    T  +GL      + F A  G+K
Sbjct: 104 TSCILEDAGSVSSYLQTVKTWLDSHPNEVVTLLI----TNGDGLDIKEFDDAFNAVNGIK 163

Query: 206 QFWF-PVSRMPKNGGDWPTVSDIIQKNQRLLVFT-SKAAKEASEGIAYVWNYMVENQYGD 265
            + F P S++    GDWPT+ ++I   +RL+VF  SKA       +   ++Y  E  +  
Sbjct: 164 DYTFAPKSKLAL--GDWPTLRELITTGKRLIVFVDSKADTNRFPYLLDEFSYYFETPFST 223

Query: 266 EGMKAGSC----PNRGE--------STPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLL 325
                  C    P  G+        +  +N     + + + F++     A  + +    +
Sbjct: 224 TDENFPQCKLDRPAGGKPDGQMYLVNHTLNVNVFGIFLPDRFKAGRTNAAVGQGSIGAQV 283

Query: 326 NMVNTCYNAAGKRWPNFIAVDFYKRSD 330
           ++ N+ Y+    R PN + +DF    D
Sbjct: 284 DLCNSIYH----RKPNVVLLDFITEGD 294

BLAST of CmoCh04G005210 vs. ExPASy TrEMBL
Match: A0A6J1FMP4 (PI-PLC X domain-containing protein At5g67130-like OS=Cucurbita moschata OX=3662 GN=LOC111447198 PE=4 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 1.7e-194
Identity = 330/342 (96.49%), Postives = 331/342 (96.78%), Query Frame = 0

Query: 22  LFHSSSAR--------KEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN 81
           LF SS A         +EGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN
Sbjct: 58  LFFSSQAAIVWVPLFLQEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN 117

Query: 82  GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND 141
           GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND
Sbjct: 118 GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND 177

Query: 142 IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA 201
           IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA
Sbjct: 178 IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA 237

Query: 202 GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG 261
           GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG
Sbjct: 238 GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG 297

Query: 262 DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG 321
           DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG
Sbjct: 298 DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG 357

Query: 322 KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK 356
           KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK
Sbjct: 358 KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK 399

BLAST of CmoCh04G005210 vs. ExPASy TrEMBL
Match: A0A6J1JDU7 (PI-PLC X domain-containing protein At5g67130-like OS=Cucurbita maxima OX=3661 GN=LOC111485972 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 3.6e-189
Identity = 314/326 (96.32%), Postives = 322/326 (98.77%), Query Frame = 0

Query: 30  KEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNGLPFNKYTWLTTHNSF 89
           +EGGTCL+N NCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN LPFNKYTWLTTHNSF
Sbjct: 23  QEGGTCLYNGNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNDLPFNKYTWLTTHNSF 82

Query: 90  AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCHDFTA 149
           AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEG+CHDFTA
Sbjct: 83  AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGQCHDFTA 142

Query: 150 FQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAAGLKQFWFPVSRMPKNG 209
           FQPAI+VLKEFQAFLGANPSEIVTIIIEDYVT PNGLTNVFAAAGL+QFWFPVS MPKNG
Sbjct: 143 FQPAIDVLKEFQAFLGANPSEIVTIIIEDYVTTPNGLTNVFAAAGLQQFWFPVSSMPKNG 202

Query: 210 GDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST 269
           GDWPTVSD+IQKN+RLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST
Sbjct: 203 GDWPTVSDMIQKNKRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST 262

Query: 270 PMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD 329
           PMNTTSRSLVIVNFFRSASNFPAAC+DNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD
Sbjct: 263 PMNTTSRSLVIVNFFRSASNFPAACRDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD 322

Query: 330 GGGAPEAVDVSNGQSICGCPTISNCK 356
           GGGAPEAVDVSNGQSICGCPTISNCK
Sbjct: 323 GGGAPEAVDVSNGQSICGCPTISNCK 348

BLAST of CmoCh04G005210 vs. ExPASy TrEMBL
Match: A0A5D3CRF2 (PI-PLC X domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G001540 PE=4 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.3e-174
Identity = 291/348 (83.62%), Postives = 315/348 (90.52%), Query Frame = 0

Query: 13  AATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKV 72
           A  LF I+FLFHSSSA KEG TC+ N NCDAGLHC+TC A+GN+RPRCTRIQPISPISKV
Sbjct: 13  ALLLFHILFLFHSSSALKEGRTCIANGNCDAGLHCETCFADGNVRPRCTRIQPISPISKV 72

Query: 73  NGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRN 132
             LPFN YTWLTTHNSFA LGAKS TG+ ILAPTNQQ SITSQLNNGVRGFMLD YDF N
Sbjct: 73  KDLPFNHYTWLTTHNSFAKLGAKSATGSLILAPTNQQDSITSQLNNGVRGFMLDMYDFEN 132

Query: 133 DIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAA 192
           DIWLCHS+ G+C+++TAFQPAI VLKEFQAFL ANP EIVTIIIEDYVT+P GLTNVF A
Sbjct: 133 DIWLCHSYGGQCYNYTAFQPAINVLKEFQAFLDANPFEIVTIIIEDYVTSPKGLTNVFGA 192

Query: 193 AGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQY 252
           AGL++FWFPV RMPKNGG+WPTVSD+IQKNQRLLVFTSKAAKEASEGIAYVW YMVENQY
Sbjct: 193 AGLRKFWFPVWRMPKNGGNWPTVSDMIQKNQRLLVFTSKAAKEASEGIAYVWRYMVENQY 252

Query: 253 GDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAA 312
           GD GMKAGSCPNRGES PMNTTSRSLVIVNFFR+A+NFP ACKDNSAPLLNMVNTC+NAA
Sbjct: 253 GDGGMKAGSCPNRGESLPMNTTSRSLVIVNFFRNAANFPEACKDNSAPLLNMVNTCHNAA 312

Query: 313 GKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 361
           G RWPNFIAVDFYKRSDGGGA +AVD++NG S+CGCP IS+CKVS YY
Sbjct: 313 GNRWPNFIAVDFYKRSDGGGAAKAVDITNGHSVCGCPNISSCKVSDYY 360

BLAST of CmoCh04G005210 vs. ExPASy TrEMBL
Match: A0A1S3C2I6 (PI-PLC X domain-containing protein At5g67130 OS=Cucumis melo OX=3656 GN=LOC103496166 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 5.2e-172
Identity = 288/348 (82.76%), Postives = 313/348 (89.94%), Query Frame = 0

Query: 13  AATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKV 72
           A  LF I+FLFHSSSA KEG TC+ N NCDAGLHC+TC A+GN+RPRCTRIQPISPISKV
Sbjct: 13  ALLLFHILFLFHSSSALKEGRTCIANGNCDAGLHCETCFADGNVRPRCTRIQPISPISKV 72

Query: 73  NGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRN 132
             LPFN YTWLTTHNSFA LGAKS TG+ ILAPTNQQ SITSQLNNGVRGFMLD YDF N
Sbjct: 73  KDLPFNHYTWLTTHNSFAKLGAKSATGSLILAPTNQQDSITSQLNNGVRGFMLDMYDFEN 132

Query: 133 DIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAA 192
           DIWLCHS+ G+C+++TAFQPAI VLKEFQAFL ANP EIVTIIIEDYVT+P GLTNVF A
Sbjct: 133 DIWLCHSYGGQCYNYTAFQPAINVLKEFQAFLDANPFEIVTIIIEDYVTSPKGLTNVFGA 192

Query: 193 AGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQY 252
           AGL++FWFPV RMPKNGG+WPTVSD+IQKNQRLLVFTSKAAKEASEGIAYVW YMVENQY
Sbjct: 193 AGLRKFWFPVWRMPKNGGNWPTVSDMIQKNQRLLVFTSKAAKEASEGIAYVWRYMVENQY 252

Query: 253 GDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAA 312
           GD GMKAGSCPNRGES PMNTTSRSLVIVNFFR+A+NFP ACKDNSAPLLNMVNTC+NAA
Sbjct: 253 GDGGMKAGSCPNRGESLPMNTTSRSLVIVNFFRNAANFPEACKDNSAPLLNMVNTCHNAA 312

Query: 313 GKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 361
           G RWPNFIAVDFYKRSDGGGA +AVD++NG S+CGCP IS+CK +  Y
Sbjct: 313 GNRWPNFIAVDFYKRSDGGGAAKAVDITNGHSVCGCPNISSCKENMRY 360

BLAST of CmoCh04G005210 vs. ExPASy TrEMBL
Match: A0A6J1D804 (PI-PLC X domain-containing protein At5g67130-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111017754 PE=4 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.5e-171
Identity = 286/356 (80.34%), Postives = 314/356 (88.20%), Query Frame = 0

Query: 1   MPRPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC 60
           M    FS+R++L  TL   +FLF SSSA KEG TC+ N NCDAGLHC+TC A+GN+RPRC
Sbjct: 55  MEHRDFSKRVILGLTLLHFVFLFDSSSALKEGRTCIVNRNCDAGLHCETCFADGNVRPRC 114

Query: 61  TRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120
           TRIQPISPIS+V  LPFN+Y WLTTHNSFA LGAKS TG+ ILAPTNQQ SITSQLNNGV
Sbjct: 115 TRIQPISPISEVKDLPFNQYAWLTTHNSFAKLGAKSATGSVILAPTNQQDSITSQLNNGV 174

Query: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180
           RG MLD YDF NDIWLCHSF G+C+++TAFQPAI VLKE QAFL ANPSEIVT+IIEDYV
Sbjct: 175 RGLMLDMYDFENDIWLCHSFGGQCYNYTAFQPAINVLKEIQAFLEANPSEIVTVIIEDYV 234

Query: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240
           T+P GLTNVF AAGL +F FPV RMPKNGG+WPTVSD+IQKNQRLLVFTSKA KEASEGI
Sbjct: 235 TSPKGLTNVFVAAGLAKFLFPVWRMPKNGGNWPTVSDMIQKNQRLLVFTSKAWKEASEGI 294

Query: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300
           AYVW Y+VENQYGDEGM+AGSCPNRGES PMNTTSRSLV++NFFRSA+NFP ACKDNSAP
Sbjct: 295 AYVWRYVVENQYGDEGMRAGSCPNRGESPPMNTTSRSLVLINFFRSAANFPEACKDNSAP 354

Query: 301 LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKV 357
           L NMVNTCYNAAGKRWPNFIAVDFYKRSDGGGA EAVD++NG SICGCP ISNCK+
Sbjct: 355 LRNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAAEAVDITNGHSICGCPNISNCKI 410

BLAST of CmoCh04G005210 vs. NCBI nr
Match: KAG6600322.1 (PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751.1 bits (1938), Expect = 4.3e-213
Identity = 358/360 (99.44%), Postives = 358/360 (99.44%), Query Frame = 0

Query: 1   MPRPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC 60
           MPRPGFSR LLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC
Sbjct: 1   MPRPGFSRGLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC 60

Query: 61  TRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120
           TRIQPISPISKVN LPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV
Sbjct: 61  TRIQPISPISKVNDLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120

Query: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180
           RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV
Sbjct: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180

Query: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240
           TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI
Sbjct: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240

Query: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300
           AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP
Sbjct: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300

Query: 301 LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 360
           LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY
Sbjct: 301 LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 360

BLAST of CmoCh04G005210 vs. NCBI nr
Match: XP_022942006.1 (PI-PLC X domain-containing protein At5g67130-like [Cucurbita moschata])

HSP 1 Score: 688.3 bits (1775), Expect = 3.4e-194
Identity = 330/342 (96.49%), Postives = 331/342 (96.78%), Query Frame = 0

Query: 22  LFHSSSAR--------KEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN 81
           LF SS A         +EGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN
Sbjct: 58  LFFSSQAAIVWVPLFLQEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN 117

Query: 82  GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND 141
           GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND
Sbjct: 118 GLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRND 177

Query: 142 IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA 201
           IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA
Sbjct: 178 IWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAA 237

Query: 202 GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG 261
           GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG
Sbjct: 238 GLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYG 297

Query: 262 DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG 321
           DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG
Sbjct: 298 DEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAG 357

Query: 322 KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK 356
           KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK
Sbjct: 358 KRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK 399

BLAST of CmoCh04G005210 vs. NCBI nr
Match: XP_022988722.1 (PI-PLC X domain-containing protein At5g67130-like [Cucurbita maxima])

HSP 1 Score: 670.6 bits (1729), Expect = 7.4e-189
Identity = 314/326 (96.32%), Postives = 322/326 (98.77%), Query Frame = 0

Query: 30  KEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNGLPFNKYTWLTTHNSF 89
           +EGGTCL+N NCDAGLHCQTCIANGNIRPRCTRIQPISPISKVN LPFNKYTWLTTHNSF
Sbjct: 23  QEGGTCLYNGNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNDLPFNKYTWLTTHNSF 82

Query: 90  AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCHDFTA 149
           AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEG+CHDFTA
Sbjct: 83  AILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGQCHDFTA 142

Query: 150 FQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAAGLKQFWFPVSRMPKNG 209
           FQPAI+VLKEFQAFLGANPSEIVTIIIEDYVT PNGLTNVFAAAGL+QFWFPVS MPKNG
Sbjct: 143 FQPAIDVLKEFQAFLGANPSEIVTIIIEDYVTTPNGLTNVFAAAGLQQFWFPVSSMPKNG 202

Query: 210 GDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST 269
           GDWPTVSD+IQKN+RLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST
Sbjct: 203 GDWPTVSDMIQKNKRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGEST 262

Query: 270 PMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD 329
           PMNTTSRSLVIVNFFRSASNFPAAC+DNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD
Sbjct: 263 PMNTTSRSLVIVNFFRSASNFPAACRDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSD 322

Query: 330 GGGAPEAVDVSNGQSICGCPTISNCK 356
           GGGAPEAVDVSNGQSICGCPTISNCK
Sbjct: 323 GGGAPEAVDVSNGQSICGCPTISNCK 348

BLAST of CmoCh04G005210 vs. NCBI nr
Match: XP_023518072.1 (PI-PLC X domain-containing protein At5g67130-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.5 bits (1713), Expect = 5.3e-187
Identity = 316/326 (96.93%), Postives = 320/326 (98.16%), Query Frame = 0

Query: 1   MPRPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC 60
           M  PGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFN NCDAGLHCQTCIANGNIRPRC
Sbjct: 1   MAPPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNGNCDAGLHCQTCIANGNIRPRC 60

Query: 61  TRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120
           TRIQPISPISKVN LPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV
Sbjct: 61  TRIQPISPISKVNDLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120

Query: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180
           RGFMLDTYDFRNDIWLCHSFEG+CHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV
Sbjct: 121 RGFMLDTYDFRNDIWLCHSFEGQCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180

Query: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240
           TAPNGLTNVFAAAGLKQFWFPVS MPKNGGDWPTVSD+IQKNQRLLVFTSKAAKEAS+GI
Sbjct: 181 TAPNGLTNVFAAAGLKQFWFPVSSMPKNGGDWPTVSDMIQKNQRLLVFTSKAAKEASDGI 240

Query: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300
           AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNT SRSLVIVNFFRSASNFPAAC+DNSAP
Sbjct: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTISRSLVIVNFFRSASNFPAACRDNSAP 300

Query: 301 LLNMVNTCYNAAGKRWPNFIAVDFYK 327
           LLNMVNTCYNAAGKRWPNFIAVDFYK
Sbjct: 301 LLNMVNTCYNAAGKRWPNFIAVDFYK 326

BLAST of CmoCh04G005210 vs. NCBI nr
Match: KAG7030979.1 (PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 641.3 bits (1653), Expect = 4.8e-180
Identity = 317/360 (88.06%), Postives = 317/360 (88.06%), Query Frame = 0

Query: 1   MPRPGFSRRLLLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRC 60
           MPRPGFSR LLLAATLFPIIFLFHSSSAR                               
Sbjct: 1   MPRPGFSRGLLLAATLFPIIFLFHSSSAR------------------------------- 60

Query: 61  TRIQPISPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120
                     KVN LPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV
Sbjct: 61  ----------KVNDLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGV 120

Query: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180
           RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV
Sbjct: 121 RGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYV 180

Query: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240
           TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI
Sbjct: 181 TAPNGLTNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGI 240

Query: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300
           AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP
Sbjct: 241 AYVWNYMVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAP 300

Query: 301 LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 360
           LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY
Sbjct: 301 LLNMVNTCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 319

BLAST of CmoCh04G005210 vs. TAIR 10
Match: AT1G49740.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 516.2 bits (1328), Expect = 2.2e-146
Identity = 241/345 (69.86%), Postives = 277/345 (80.29%), Query Frame = 0

Query: 11  LLAATLFPIIFLFHSSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPIS 70
           LL A L    FL   SSA KEG TC+ NSNCDAGLHC+TCIAN + RPRC+R QPI+PI+
Sbjct: 9   LLIALLLQSSFLLEISSALKEGKTCITNSNCDAGLHCETCIANTDFRPRCSRTQPINPIT 68

Query: 71  KVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDF 130
           K  GLPFNKY+WLTTHNSFA LG  S TG+ ILAPTNQQ SITSQLNNGVRGFMLD YDF
Sbjct: 69  KAKGLPFNKYSWLTTHNSFARLGEVSRTGSAILAPTNQQDSITSQLNNGVRGFMLDMYDF 128

Query: 131 RNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVF 190
           +NDIWLCHSF+G C +FTAFQPAI +L+EFQ FL  N  E+VTIIIEDYV +P GLT VF
Sbjct: 129 QNDIWLCHSFDGTCFNFTAFQPAINILREFQVFLEKNKEEVVTIIIEDYVKSPKGLTKVF 188

Query: 191 AAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVEN 250
            AAGL++F FPVSRMPKNGGDWP + D+++KNQRLLVFTS + KEA+EGIAY W YMVEN
Sbjct: 189 DAAGLRKFMFPVSRMPKNGGDWPRLDDMVRKNQRLLVFTSDSHKEATEGIAYQWKYMVEN 248

Query: 251 QYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYN 310
           QYG+ G+K G CPNR +S PM+  S+SLV+VN F  A++   ACK NSA LL  + TCY 
Sbjct: 249 QYGNGGLKVGVCPNRAQSAPMSDKSKSLVLVNHFPDAADVIVACKQNSASLLESIKTCYQ 308

Query: 311 AAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCK 356
           AAG+RWPNFIAVDFYKRSDGGGAP+AVDV+NG  ICGC   + CK
Sbjct: 309 AAGQRWPNFIAVDFYKRSDGGGAPQAVDVANGNLICGCDNFAACK 353

BLAST of CmoCh04G005210 vs. TAIR 10
Match: AT3G19310.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 491.1 bits (1263), Expect = 7.5e-139
Identity = 227/350 (64.86%), Postives = 276/350 (78.86%), Query Frame = 0

Query: 12  LAATLFPIIFLFHSSS-ARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPIS 71
           L A L P I +  S S A KEG TC+ + NCD GLHC++C+A+ + RPRC+R+QPI+P +
Sbjct: 9   LTALLIPCILIILSPSYALKEGETCIVSKNCDRGLHCESCLASDSFRPRCSRMQPINPTT 68

Query: 72  KVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDF 131
           KV GLP+NKY+WLTTHNSFA +GAKS TG+ ILAP+NQQ SITSQL NGVRGFMLD YDF
Sbjct: 69  KVKGLPYNKYSWLTTHNSFARMGAKSGTGSMILAPSNQQDSITSQLLNGVRGFMLDLYDF 128

Query: 132 RNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVF 191
           +NDIWLCHS+ G C ++TAFQPA+ +LKEFQ FL  N   +VT+I+EDYV +PNGLT VF
Sbjct: 129 QNDIWLCHSYGGNCFNYTAFQPAVNILKEFQVFLDKNKDVVVTLILEDYVKSPNGLTRVF 188

Query: 192 AAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVEN 251
            A+GL+ F FPVSRMPKNG DWPT+ D+I +NQRLLVFTS   KEASEGIA++W YM+EN
Sbjct: 189 DASGLRNFMFPVSRMPKNGEDWPTLDDMICQNQRLLVFTSNPQKEASEGIAFMWRYMIEN 248

Query: 252 QYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYN 311
           QYGD GMKAG C NR ES  M   SRSL++VN+F   ++   +CK NSAPLL+ V  C  
Sbjct: 249 QYGDGGMKAGVCTNRPESVAMGDRSRSLILVNYFPDTADVIGSCKQNSAPLLDTVKNCQE 308

Query: 312 AAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 361
           A+GKRWPNFIAVDFYKRSDGGGAP+AVDV+NG ++CGC  I+ CK +  Y
Sbjct: 309 ASGKRWPNFIAVDFYKRSDGGGAPKAVDVANGHAVCGCEDIAACKENMPY 358

BLAST of CmoCh04G005210 vs. TAIR 10
Match: AT4G36945.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.4e-129
Identity = 213/324 (65.74%), Postives = 257/324 (79.32%), Query Frame = 0

Query: 32  GGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPISKVNGLPFNKYTWLTTHNSFAI 91
           G TC   S CDAGL CQ+C ANGN    CTRIQP++P SKVNGLPFNKY+WLTTHNS+AI
Sbjct: 26  GETCSSTSECDAGLSCQSCPANGNTGSTCTRIQPLNPTSKVNGLPFNKYSWLTTHNSYAI 85

Query: 92  LGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYDFRNDIWLCHSFEGKCHDFTAFQ 151
            GA S TG+ +++P NQ+ SIT+QL NGVRG MLDTYDF+NDIWLCHS  G C +FTAFQ
Sbjct: 86  TGANSATGSFLVSPKNQEDSITNQLKNGVRGIMLDTYDFQNDIWLCHSTGGTCFNFTAFQ 145

Query: 152 PAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNVFAAAGLKQFWFPVSRMPKNGGD 211
           PAI  LKE   FL +N SEIVTII+EDYV +  GLTNVF A+GL +F  P+SRMPK+G D
Sbjct: 146 PAINALKEINDFLESNLSEIVTIILEDYVKSQMGLTNVFNASGLSKFLLPISRMPKDGTD 205

Query: 212 WPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVENQYGDEGMKAGSCPNRGESTPM 271
           WPTV D++++NQRL+VFTSK  KEASEG+AY WNYMVENQYG++GMK GSC +R ES+ +
Sbjct: 206 WPTVDDMVKQNQRLVVFTSKKDKEASEGLAYQWNYMVENQYGNDGMKDGSCSSRSESSSL 265

Query: 272 NTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCYNAAGKRWPNFIAVDFYKRSDGG 331
           +T SRSLV  N+F ++ N   AC DNS+PL+ M+ TC+ AAGKRWPNFIAVDFY+RSD G
Sbjct: 266 DTMSRSLVFQNYFETSPNSTQACADNSSPLIEMMRTCHEAAGKRWPNFIAVDFYQRSDSG 325

Query: 332 GAPEAVDVSNGQSICGCPTISNCK 356
           GA EAVD +NG+  CGC ++  CK
Sbjct: 326 GAAEAVDEANGRLTCGCDSLVYCK 349

BLAST of CmoCh04G005210 vs. TAIR 10
Match: AT5G67130.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 3.5e-112
Identity = 193/354 (54.52%), Postives = 244/354 (68.93%), Query Frame = 0

Query: 12  LAATLFPIIFLFHSSSARKEGG-----TCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPI 71
           +  +L  ++  F  SSA   G      +C   ++C +GL+C  C A G  +P CTR Q  
Sbjct: 12  VTVSLLLLLLSFSFSSACSNGNCQLLDSCSSATDCVSGLYCGDCPAVGRSKPVCTRGQAT 71

Query: 72  SPISKVNGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLD 131
           SP S +NGLPFNKYTWL THN+F+   A    G   +   NQ+ +IT+QL NGVRG MLD
Sbjct: 72  SPTSIINGLPFNKYTWLMTHNAFSNANAPLLPGVERITFYNQEDTITNQLQNGVRGLMLD 131

Query: 132 TYDFRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGL 191
            YDF NDIWLCHS  G+C +FTAFQPAI +L+E +AFL  NP+EIVTIIIEDYV  P GL
Sbjct: 132 MYDFNNDIWLCHSLRGQCFNFTAFQPAINILREVEAFLSQNPTEIVTIIIEDYVHRPKGL 191

Query: 192 TNVFAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNY 251
           + +FA AGL ++WFPVS+MP+ G DWPTV+D++Q+N RLLVFTS AAKE  EG+AY W Y
Sbjct: 192 STLFANAGLDKYWFPVSKMPRKGEDWPTVTDMVQENHRLLVFTSVAAKEDEEGVAYQWRY 251

Query: 252 MVENQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVN 311
           MVEN+ GD G+K GSCPNR ES P+N+ S SL ++N+F +      ACK++SAPL  MV 
Sbjct: 252 MVENESGDPGVKRGSCPNRKESQPLNSKSSSLFLMNYFPTYPVEKDACKEHSAPLAEMVG 311

Query: 312 TCYNAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNCKVSHYY 361
           TC  + G R PNF+AV+FY RSDGGG  E +D  NG  +CGC T+S C+    Y
Sbjct: 312 TCLKSGGNRMPNFLAVNFYMRSDGGGVFEILDRMNGPVLCGCETLSACQPGAAY 365

BLAST of CmoCh04G005210 vs. TAIR 10
Match: AT1G13680.1 (PLC-like phosphodiesterases superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 4.8e-93
Identity = 168/345 (48.70%), Postives = 218/345 (63.19%), Query Frame = 0

Query: 19  IIFLFH--------SSSARKEGGTCLFNSNCDAGLHCQTCIANGNIRPRCTRIQPISPIS 78
           I+ LFH        S  + + G  C  + +C+ GL C  C   G    RC R       S
Sbjct: 12  ILVLFHPAAITFVASYGSLQLGDQCSSDEDCNVGLGCFKC---GIDVARCVRSNITDQFS 71

Query: 79  KV-NGLPFNKYTWLTTHNSFAILGAKSETGTPILAPTNQQGSITSQLNNGVRGFMLDTYD 138
            V N +PFNKY +LTTHNS+AI G      T       Q+ +I  QLN+GVR  MLDTYD
Sbjct: 72  IVNNSMPFNKYAFLTTHNSYAIEGKALHVAT-------QEDTIVQQLNSGVRALMLDTYD 131

Query: 139 FRNDIWLCHSFEGKCHDFTAFQPAIEVLKEFQAFLGANPSEIVTIIIEDYVTAPNGLTNV 198
           +  D+W CHSF+ +C +FT F  AI+  KE  AFL ANPSEIVT+I+EDYV + NGLT V
Sbjct: 132 YEGDVWFCHSFDEQCFEFTKFNRAIDTFKEIFAFLTANPSEIVTLILEDYVKSQNGLTKV 191

Query: 199 FAAAGLKQFWFPVSRMPKNGGDWPTVSDIIQKNQRLLVFTSKAAKEASEGIAYVWNYMVE 258
           F  +GLK+FWFPV  MP  G DWP V D++  N RL+VFTS  +K+ +EGIAY WNYMVE
Sbjct: 192 FTDSGLKKFWFPVQNMPIGGQDWPLVKDMVANNHRLIVFTSAKSKQETEGIAYQWNYMVE 251

Query: 259 NQYGDEGMKAGSCPNRGESTPMNTTSRSLVIVNFFRSASNFPAACKDNSAPLLNMVNTCY 318
           NQYGD+G+K   C NR +S  +   +++LV VN F++       C++NS  LL+M+ TCY
Sbjct: 252 NQYGDDGVKPDECSNRADSALLTDKTKALVSVNHFKTVPVKILTCEENSEQLLDMIKTCY 311

Query: 319 NAAGKRWPNFIAVDFYKRSDGGGAPEAVDVSNGQSICGCPTISNC 355
            AAG RW NF+AV+FYKRS+GGG  +A+D  NG+ +CG   +  C
Sbjct: 312 VAAGNRWANFVAVNFYKRSNGGGTFQAIDKLNGELLCGRDDVHAC 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93XX55.0e-11154.52PI-PLC X domain-containing protein At5g67130 OS=Arabidopsis thaliana OX=3702 GN=... [more]
D4AUX69.3e-0925.74Uncharacterized secreted protein ARB_08043 OS=Arthroderma benhamiae (strain ATCC... [more]
D4AK171.0e-0723.97PI-PLC X domain-containing protein 1 OS=Arthroderma benhamiae (strain ATCC MYA-4... [more]
Match NameE-valueIdentityDescription
A0A6J1FMP41.7e-19496.49PI-PLC X domain-containing protein At5g67130-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1JDU73.6e-18996.32PI-PLC X domain-containing protein At5g67130-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A5D3CRF23.3e-17483.62PI-PLC X domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C2I65.2e-17282.76PI-PLC X domain-containing protein At5g67130 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1D8047.5e-17180.34PI-PLC X domain-containing protein At5g67130-like isoform X4 OS=Momordica charan... [more]
Match NameE-valueIdentityDescription
KAG6600322.14.3e-21399.44PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022942006.13.4e-19496.49PI-PLC X domain-containing protein At5g67130-like [Cucurbita moschata][more]
XP_022988722.17.4e-18996.32PI-PLC X domain-containing protein At5g67130-like [Cucurbita maxima][more]
XP_023518072.15.3e-18796.93PI-PLC X domain-containing protein At5g67130-like isoform X1 [Cucurbita pepo sub... [more]
KAG7030979.14.8e-18088.06PI-PLC X domain-containing protein, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
AT1G49740.12.2e-14669.86PLC-like phosphodiesterases superfamily protein [more]
AT3G19310.17.5e-13964.86PLC-like phosphodiesterases superfamily protein [more]
AT4G36945.12.4e-12965.74PLC-like phosphodiesterases superfamily protein [more]
AT5G67130.13.5e-11254.52PLC-like phosphodiesterases superfamily protein [more]
AT1G13680.14.8e-9348.70PLC-like phosphodiesterases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilyGENE3D3.20.20.190Phosphatidylinositol (PI) phosphodiesterasecoord: 67..332
e-value: 7.8E-27
score: 96.5
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 74..342
NoneNo IPR availablePANTHERPTHR13593:SF89PLC-LIKE PHOSPHODIESTERASES SUPERFAMILY PROTEINcoord: 14..357
NoneNo IPR availablePANTHERPTHR13593UNCHARACTERIZEDcoord: 14..357
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 76..181
score: 9.873
NoneNo IPR availableCDDcd08588PI-PLCc_At5g67130_likecoord: 67..341
e-value: 2.5812E-105
score: 308.49

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G005210.1CmoCh04G005210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0008081 phosphoric diester hydrolase activity