CmoCh04G004950 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G004950
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAuxin-responsive GH3 family protein
LocationCmo_Chr04: 2470212 .. 2472488 (-)
RNA-Seq ExpressionCmoCh04G004950
SyntenyCmoCh04G004950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCACTCATCCACCACACCCCTTCTTCTTCTTCTTCGTCTCCTACATCTTCTTCTTCTTCTCCAATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGTCCTCCTGCCTGCGAAAAAGACGCAAAGGCTCTTCAATTCATCGAACAAATGACTCGAAACGCCGACGCCGTCCAGCGCACCGTCCTCGCCGAAATCTTGACTAGAAATGCCTCCACAGAGTACCTCCGCCGCTACTTCCTTGCCGGCGCTACAGATCCTAACACCTTTAAAGACAAGCTTCCTGTCATCACTTACGAAGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCTCCTATCCTCTCCTCCCATCCTATCTCCGAGTTCCTCACCAGGTAAATTACACTGAAATCTCTTCATCGGAGGAATTAGGAATTTCAAATAGTTGAATCGATTTTCTGATGCGGTGGTTTTTGCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAACTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTGTAAGTATAAGCAAAGCAAGGATCATCAAAATCAAAGCTAATTTGAAAATGAATTATTGTTTGTTATTTTATTGAAAATTATAAGCGCGTAAAATTTAATTTCTTATTGCTTTCTGACAGCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTAACCAGTTACTATAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTATATGGTCTATACGAGCCCTAATGAAGCCATACTCTGCCCCGATTCCTTCCAGAGCATGTATACGCAGATGCTCTGCGGCCTTCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGCGCGATCCGGTTCCTACAGTTGAACTGGTCCGATCTTGCCAACGATATCCGGACTGGAAAATTGAGTACGAAGATCACCGATCCGTCGTTGAGAGATTGTATCGAGAAGATCCTGAAACCAGATGAGGAATCGGCGGATTTCATTTCGGAGGAATGTTCGAAGGAGAATTGGGAGGGAATCGTCACGAGGATTTGGCCGAAAACGAAATACCTAGACGTGATCGTCACTGGAGCGATGGCGCAATACATTCCAACGCTCGATTACTACAGCGGCGGATTGCCTCTGGCGTGCACGATGTACGCCTCATCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCAGAGGTTTCCTACACAATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCAAATTCCGGAAACGATTCAGCGCCGAAACTCGTCGACCTAGTCGACGTAGAGGTCGGAAAAGAATACGAACTCGTAATCACTACATACGCCGGACTATACCGTTACCGAGTCGGCGACATACTCCGAGTAACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTACTGAGCATCGACTCAGACAAAACAGACGAAGCAGAGTTGCAGAAAGCTGTGGAGAACGCATCAAAACTCCTGAAGGAAGTTAAAACAAGCGTAGTGGAGTACACAAGTTACGCCGATACAACGACAATTCCAGGGCACTACGTGATATACTGGGAACTAATGGTGAAAGAAACAGAAGGGAAATGGCCAAGCAAGGAGACGATGGAACAGTGCTGCTTGGCAATGGAAGAATCACTAAACTCCGTTTACCGGCAAGGACGAGTCGCAGACAATTCAATCGGAGCTTTAGAAATCAGAGTGGTGAAGAGCGGAACATTCGAAGAATTGATGGATTACGCTATCTCCCGAGGAGCGTCCATCAACCAGTACAAGGTGCCAAGGTGCGTGAATTTTACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCCGCTCTGCCACGTTGGACTCCAGCTAGGAGACGCTGATTTATTAATTTAATTTTCTTTTAAATTAATTAATTTGAGAAAGAAAATGTAAGTGGGAAGAATTTAGGATGCATTAGAAGTCTGTATTGTATTTGGTTCTCATAACTAAATGAAAAATCACATTTATTTACTTTATCCCAACATTTGATTAATAAACAAAAATTAGAATCTATAATTTTAAATGAAAAGGTGGTGTCCAATAAGTGTTGTTTTAGTA

mRNA sequence

TCTCACTCATCCACCACACCCCTTCTTCTTCTTCTTCGTCTCCTACATCTTCTTCTTCTTCTCCAATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGTCCTCCTGCCTGCGAAAAAGACGCAAAGGCTCTTCAATTCATCGAACAAATGACTCGAAACGCCGACGCCGTCCAGCGCACCGTCCTCGCCGAAATCTTGACTAGAAATGCCTCCACAGAGTACCTCCGCCGCTACTTCCTTGCCGGCGCTACAGATCCTAACACCTTTAAAGACAAGCTTCCTGTCATCACTTACGAAGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCTCCTATCCTCTCCTCCCATCCTATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAACTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTAACCAGTTACTATAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTATATGGTCTATACGAGCCCTAATGAAGCCATACTCTGCCCCGATTCCTTCCAGAGCATGTATACGCAGATGCTCTGCGGCCTTCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGCGCGATCCGGTTCCTACAGTTGAACTGGTCCGATCTTGCCAACGATATCCGGACTGGAAAATTGAGTACGAAGATCACCGATCCGTCGTTGAGAGATTGTATCGAGAAGATCCTGAAACCAGATGAGGAATCGGCGGATTTCATTTCGGAGGAATGTTCGAAGGAGAATTGGGAGGGAATCGTCACGAGGATTTGGCCGAAAACGAAATACCTAGACGTGATCGTCACTGGAGCGATGGCGCAATACATTCCAACGCTCGATTACTACAGCGGCGGATTGCCTCTGGCGTGCACGATGTACGCCTCATCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCAGAGGTTTCCTACACAATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCAAATTCCGGAAACGATTCAGCGCCGAAACTCGTCGACCTAGTCGACGTAGAGGTCGGAAAAGAATACGAACTCGTAATCACTACATACGCCGGACTATACCGTTACCGAGTCGGCGACATACTCCGAGTAACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTACTGAGCATCGACTCAGACAAAACAGACGAAGCAGAGTTGCAGAAAGCTGTGGAGAACGCATCAAAACTCCTGAAGGAAGTTAAAACAAGCGTAGTGGAGTACACAAGTTACGCCGATACAACGACAATTCCAGGGCACTACGTGATATACTGGGAACTAATGGTGAAAGAAACAGAAGGGAAATGGCCAAGCAAGGAGACGATGGAACAGTGCTGCTTGGCAATGGAAGAATCACTAAACTCCGTTTACCGGCAAGGACGAGTCGCAGACAATTCAATCGGAGCTTTAGAAATCAGAGTGGTGAAGAGCGGAACATTCGAAGAATTGATGGATTACGCTATCTCCCGAGGAGCGTCCATCAACCAGTACAAGGTGCCAAGGTGCGTGAATTTTACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCCGCTCTGCCACGTTGGACTCCAGCTAGGAGACGCTGATTTATTAATTTAATTTTCTTTTAAATTAATTAATTTGAGAAAGAAAATGTAAGTGGGAAGAATTTAGGATGCATTAGAAGTCTGTATTGTATTTGGTTCTCATAACTAAATGAAAAATCACATTTATTTACTTTATCCCAACATTTGATTAATAAACAAAAATTAGAATCTATAATTTTAAATGAAAAGGTGGTGTCCAATAAGTGTTGTTTTAGTA

Coding sequence (CDS)

ATGGCTGTCGATTCTTCTCTCTCTTCACCTCTCGGTCCTCCTGCCTGCGAAAAAGACGCAAAGGCTCTTCAATTCATCGAACAAATGACTCGAAACGCCGACGCCGTCCAGCGCACCGTCCTCGCCGAAATCTTGACTAGAAATGCCTCCACAGAGTACCTCCGCCGCTACTTCCTTGCCGGCGCTACAGATCCTAACACCTTTAAAGACAAGCTTCCTGTCATCACTTACGAAGATCTCCAGCCCGAAATCCAACGGATTGCCAGTGGAGATCGCTCTCCTATCCTCTCCTCCCATCCTATCTCCGAGTTCCTCACCAGTTCTGGTACTTCTGCCGGTGAGAGGAAGCTTATGCCGACGATTAAGGAGGAACTGGATCGTCGTCAACTTCTGTACAGTCTTCTCATGCCAGTTATGAACCTCTATGTGCCTGGGTTGGATAAAGGCAAGGGGCTTTACTTCTTGTTTGTGAAATCGGAGACGAGGACTCCGGGTGGGCTCTTGGCCCGACCCGTTTTAACCAGTTACTATAAGAGCGAACACTTCAAGACCCGACCCTACGACCCGTATATGGTCTATACGAGCCCTAATGAAGCCATACTCTGCCCCGATTCCTTCCAGAGCATGTATACGCAGATGCTCTGCGGCCTTCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTCTTCGCCTCCGGCCTACTCCGCGCGATCCGGTTCCTACAGTTGAACTGGTCCGATCTTGCCAACGATATCCGGACTGGAAAATTGAGTACGAAGATCACCGATCCGTCGTTGAGAGATTGTATCGAGAAGATCCTGAAACCAGATGAGGAATCGGCGGATTTCATTTCGGAGGAATGTTCGAAGGAGAATTGGGAGGGAATCGTCACGAGGATTTGGCCGAAAACGAAATACCTAGACGTGATCGTCACTGGAGCGATGGCGCAATACATTCCAACGCTCGATTACTACAGCGGCGGATTGCCTCTGGCGTGCACGATGTACGCCTCATCCGAATGTTATTTCGGACTGAATCTGAACCCTATGTGCAAGCCATCAGAGGTTTCCTACACAATAATGCCAAACATGGCCTATTTCGAGTTCCTCCCTCACGAGCCAAATTCCGGAAACGATTCAGCGCCGAAACTCGTCGACCTAGTCGACGTAGAGGTCGGAAAAGAATACGAACTCGTAATCACTACATACGCCGGACTATACCGTTACCGAGTCGGCGACATACTCCGAGTAACCGGATTCCACAACGCAGCGCCGCAGTTCCACTTCGTGAGGAGGAAGAACGTGGTACTGAGCATCGACTCAGACAAAACAGACGAAGCAGAGTTGCAGAAAGCTGTGGAGAACGCATCAAAACTCCTGAAGGAAGTTAAAACAAGCGTAGTGGAGTACACAAGTTACGCCGATACAACGACAATTCCAGGGCACTACGTGATATACTGGGAACTAATGGTGAAAGAAACAGAAGGGAAATGGCCAAGCAAGGAGACGATGGAACAGTGCTGCTTGGCAATGGAAGAATCACTAAACTCCGTTTACCGGCAAGGACGAGTCGCAGACAATTCAATCGGAGCTTTAGAAATCAGAGTGGTGAAGAGCGGAACATTCGAAGAATTGATGGATTACGCTATCTCCCGAGGAGCGTCCATCAACCAGTACAAGGTGCCAAGGTGCGTGAATTTTACTCCCATAATGGAACTTCTCGATTCTAGAGTAGTATCGGCGCATTTTAGTCCCGCTCTGCCACGTTGGACTCCAGCTAGGAGACGCTGA

Protein sequence

MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLAGATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIPGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR
Homology
BLAST of CmoCh04G004950 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 4.7e-284
Identity = 485/597 (81.24%), Postives = 533/597 (89.28%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDS+LSSPLGPPACEKDAKAL+FIE+MTRNAD VQ  +LAEIL RNA TEYLRR+ L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD +TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDPY VYTSPNEAILCPDSFQSMYTQMLCGLL+R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQL+WS  A+DI  G L ++ITDPS+R C+  ILKPD   A+FI  EC  +NWE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLL-KEVKTSVVEYTSYADTTTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT+TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           PGHYV+YWEL+V++   + PS ET+ +CCL MEESLNSVYRQ RVADNS+G LEIRVV++
Sbjct: 481 PGHYVLYWELLVRD-GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LP WTP RRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of CmoCh04G004950 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 2.7e-263
Identity = 458/598 (76.59%), Postives = 516/598 (86.29%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           M VDS+L SP+      KD KAL+FIE+MTRN D VQ+ V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKSE
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
            FK RP DPY VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKIL-KPDEESADFISEECSKEN-WEGIVT 300
           LQ NW +LA+DI TG LS++I+DP++++ + KIL KPD+E ADFI+  C ++N WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH       S  +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+T T
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540
           IPGHYVIYWEL+VK+     P+ E M +CCL MEESLNSVYRQ RVAD SIG LEIRVVK
Sbjct: 481 IPGHYVIYWELLVKDQTNP-PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           +GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALP W+P RRR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of CmoCh04G004950 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 879.8 bits (2272), Expect = 1.8e-254
Identity = 438/592 (73.99%), Postives = 494/592 (83.45%), Query Frame = 0

Query: 5   SSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLAGATD 64
           ++L +P      E D + L+FI++MT N DAVQ  VL EIL RNA TEYL +  L GATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+ K+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKS+HFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDPY  YTSP  AILC D+FQSMY QM+CGL +R+ VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRIWPKT 304
           W  LA+DI +G+L+ ++TDPS+R+ +  IL PD E A  I  ECSK +W GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E  + +  A +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+    SVVEYTS A T  IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFE 544
           VIYWEL+ K         +T+ +CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFE
Sbjct: 492 VIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFE 551

Query: 545 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARR 596
           ELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALP WTPARR
Sbjct: 552 ELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of CmoCh04G004950 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 1.8e-254
Identity = 438/592 (73.99%), Postives = 494/592 (83.45%), Query Frame = 0

Query: 5   SSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLAGATD 64
           ++L +P      E D + L+FI++MT N DAVQ  VL EIL RNA TEYL +  L GATD
Sbjct: 12  TALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATD 71

Query: 65  PNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEE 124
              F+ K+PV++Y+DLQP IQRIA+GDRSPILS+HP+SEFLTSSGTSAGERKLMPTI +E
Sbjct: 72  RAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDE 131

Query: 125 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKT 184
           LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSET+TPGGL ARPVLTSYYKS+HFK 
Sbjct: 132 LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKN 191

Query: 185 RPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLN 244
           RPYDPY  YTSP  AILC D+FQSMY QM+CGL +R+ VLRLGAVFASGLLRAIRFLQLN
Sbjct: 192 RPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLN 251

Query: 245 WSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRIWPKT 304
           W  LA+DI +G+L+ ++TDPS+R+ +  IL PD E A  I  ECSK +W GI+TR+WP T
Sbjct: 252 WEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNT 311

Query: 305 KYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNM 364
           KYLDVIVTGAMAQYIPTL++YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM
Sbjct: 312 KYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNM 371

Query: 365 AYFEFLP-HEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNA 424
            YFEFLP  E  + +  A +LVDL  VEVG+EYELVITTYAGL RYRVGD+LRVTGFHNA
Sbjct: 372 GYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRVTGFHNA 431

Query: 425 APQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIPGHY 484
           APQF FVRRKNV+LSI+SDKTDEAELQ+AVE AS LL+    SVVEYTS A T  IPGHY
Sbjct: 432 APQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTKRIPGHY 491

Query: 485 VIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFE 544
           VIYWEL+ K         +T+ +CCL MEE+LN+VYRQ RVAD SIG LEIRVV+ GTFE
Sbjct: 492 VIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFE 551

Query: 545 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARR 596
           ELMDYAISRGASINQYKVPRCV F PI+ELLDSRVVS+HFSPALP WTPARR
Sbjct: 552 ELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHFSPALPHWTPARR 603

BLAST of CmoCh04G004950 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 3.7e-252
Identity = 430/592 (72.64%), Postives = 500/592 (84.46%), Query Frame = 0

Query: 15  ACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLAGATDPNTFKDKLPV 74
           ACE+DA+ L+FIE+MTR  DAVQ  VLA IL RN   EYLRR+ + G TD   FK ++PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 75  ITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSL 134
           +TYEDL+PEI+RIA+GDRS I+SSHPI+EFLTSSGTSAGERKLMPTI++ELDRRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 135 LMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPYMVYT 194
           LMPVMNLYVPGLDKGKGLYFLF+KSET+TPGGL ARPVLTSYYKS+HFK RP+DPY VYT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 195 SPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLANDIRT 254
           SP  AILC D+FQSMY QMLCGL+ R +VLR+GAVFASGLLRAIRFLQL+W +LA+DIRT
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 255 GKLSTKITDPSLRDCIEKIL-KPDEESADFISEECSKENWEGIVTRIWPKTKYLDVIVTG 314
           G LS K+T+PS+RD + ++L  PD E A F+  EC K+ WEGI+TR+WP TKYLDVIVTG
Sbjct: 265 GTLSAKVTEPSIRDAVAEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVTG 324

Query: 315 AMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHE 374
           AMAQYIPTL +YSGGLP+ACTMYASSECYFGLNL PMC PSEVSYTIMPNM YFE +PH+
Sbjct: 325 AMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPHD 384

Query: 375 PNS----GNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAAPQFHF 434
           P++     +   P+LVDL D EVG+EYELVITTYAGL RYRVGDIL+VTGFHNAAPQF F
Sbjct: 385 PDAPPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQFRF 444

Query: 435 VRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIPGHYVIYWEL 494
           VRRKNV+LSIDSDKTDEAELQ AVE AS LL     S+VEYTS AD TTIPGHYV+YWEL
Sbjct: 445 VRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVYWEL 504

Query: 495 MVKETEGKWP------SKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFE 554
           MV+E  G WP       +   E+CCL MEE+LN+VYRQGR  + +IG LEIRVV++GTFE
Sbjct: 505 MVRE-GGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGTFE 564

Query: 555 ELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARR 596
           E+MDYAISRGASINQYK PRCV+F PI+ELL+SRV+S HFSPA P+++P ++
Sbjct: 565 EVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

BLAST of CmoCh04G004950 vs. ExPASy TrEMBL
Match: A0A6J1FTR9 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111448040 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

BLAST of CmoCh04G004950 vs. ExPASy TrEMBL
Match: A0A6J1IUJ6 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111479427 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 593/596 (99.50%), Postives = 595/596 (99.83%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNWSDLANDIRTGKLS+KITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSG+DSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARRR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 596

BLAST of CmoCh04G004950 vs. ExPASy TrEMBL
Match: A0A6J1E8A0 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111430783 PE=3 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 559/596 (93.79%), Postives = 581/596 (97.48%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK+KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNW DLANDIR G L++KITDPSLRDCIEKILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKE EGKWPSKE ME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of CmoCh04G004950 vs. ExPASy TrEMBL
Match: A0A6J1J7V1 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111483372 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 558/596 (93.62%), Postives = 579/596 (97.15%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TDP+TFK KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GTTDPHTFKGKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNW DLANDIR G L+TKITDPSLRDCIEKILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKE EGKWPSKE ME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRV+SAHFSPALPRWTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVISAHFSPALPRWTPARTR 596

BLAST of CmoCh04G004950 vs. ExPASy TrEMBL
Match: A0A5A7T148 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold379G001170 PE=3 SV=1)

HSP 1 Score: 1100.5 bits (2845), Expect = 0.0e+00
Identity = 546/600 (91.00%), Postives = 571/600 (95.17%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAV SSLSSPLGPPACE DAK LQFI++MTRNADAVQ TVLAEIL+RNASTEYLRRY L 
Sbjct: 1   MAVHSSLSSPLGPPACETDAKPLQFIDEMTRNADAVQHTVLAEILSRNASTEYLRRYSLD 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP TFK KLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPQTFKAKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP+MVYTSPNEAILCPDSFQSMYTQMLCGLL+R+QVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFMVYTSPNEAILCPDSFQSMYTQMLCGLLQRNQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNW DLA DIR G L+++ITDPSLRDCI KILKPD + ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWHDLATDIRNGTLNSRITDPSLRDCILKILKPDSQLADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLD+YSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDFYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLP EPNSG+DS+PKLVDLVDVE+GKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPLEPNSGSDSSPKLVDLVDVEIGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENAS+LLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASELLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKET-----EGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIR 540
           GHYVIYWELMVKE      +GKWPSKE ME+CCL MEES+NSVYRQGRVAD+SIGALEIR
Sbjct: 481 GHYVIYWELMVKEAGDQEGKGKWPSKEVMEECCLRMEESMNSVYRQGRVADHSIGALEIR 540

Query: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARR 596
           VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVS HFSPALP WTPAR+
Sbjct: 541 VVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSTHFSPALPHWTPARK 600

BLAST of CmoCh04G004950 vs. NCBI nr
Match: XP_022943254.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

BLAST of CmoCh04G004950 vs. NCBI nr
Match: XP_023519906.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo] >KAG6600299.1 putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030958.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 594/596 (99.66%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNWSDLANDIRTGKLS+KITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSG+DSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 596

BLAST of CmoCh04G004950 vs. NCBI nr
Match: XP_022979865.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 593/596 (99.50%), Postives = 595/596 (99.83%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNWSDLANDIRTGKLS+KITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI
Sbjct: 241 LQLNWSDLANDIRTGKLSSKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPNSG+DSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNSGSDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG
Sbjct: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALP WTPARRR
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPHWTPARRR 596

BLAST of CmoCh04G004950 vs. NCBI nr
Match: XP_022922958.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 559/596 (93.79%), Postives = 581/596 (97.48%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK+KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNW DLANDIR G L++KITDPSLRDCIEKILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNSKITDPSLRDCIEKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKE EGKWPSKE ME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARTR 596

BLAST of CmoCh04G004950 vs. NCBI nr
Match: XP_023552180.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 558/596 (93.62%), Postives = 581/596 (97.48%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQ+TVLAEILTRNA+TEYLRRY L 
Sbjct: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQQTVLAEILTRNAATEYLRRYSLD 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATDP+TFK+KLPVITYEDL+P+IQRIA+GDRSPILSSHPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDPHTFKEKLPVITYEDLEPDIQRIANGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS+
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSD 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HFKTRPYDP++VYTSPNEAILCPDSFQSMYTQMLCGLL+RHQVLRLGAVFASGLLRAIRF
Sbjct: 181 HFKTRPYDPFVVYTSPNEAILCPDSFQSMYTQMLCGLLQRHQVLRLGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQLNW DLANDIR G L+TKITDPSLRDCI+KILKPD E ADF+S+ECSKE+WEGIVTRI
Sbjct: 241 LQLNWQDLANDIRNGTLNTKITDPSLRDCIDKILKPDAELADFVSDECSKEDWEGIVTRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI
Sbjct: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEFLPHEPN+ +DS PKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFLPHEPNTESDSPPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIP 480
           HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKE K SVVEYTSYA+T TIP
Sbjct: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEWKASVVEYTSYAETKTIP 480

Query: 481 GHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSG 540
           GHYVIYWELMVKE EGKWPSKE ME+CCLAMEESLNSVYRQGRVAD+SIGALEIRVVK+G
Sbjct: 481 GHYVIYWELMVKEAEGKWPSKEVMEECCLAMEESLNSVYRQGRVADHSIGALEIRVVKNG 540

Query: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSR+VSAHFSPALPRWTPAR R
Sbjct: 541 TFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRLVSAHFSPALPRWTPARTR 596

BLAST of CmoCh04G004950 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 978.0 bits (2527), Expect = 3.4e-285
Identity = 485/597 (81.24%), Postives = 533/597 (89.28%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           MAVDS+LSSPLGPPACEKDAKAL+FIE+MTRNAD VQ  +LAEIL RNA TEYLRR+ L 
Sbjct: 1   MAVDSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLC 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           GATD +TFK K+PVITYEDLQPEIQRIA GDRSPILS+HPISEFLTSSGTSAGERKLMPT
Sbjct: 61  GATDRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           IKEELDRRQLLYSLLMPVMNLYVPGLDKGKG+YFLFVKSET+TPGGL ARPVLTSYYKSE
Sbjct: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
           HF++RPYDPY VYTSPNEAILCPDSFQSMYTQMLCGLL+R  VLR+GAVFASGLLRAIRF
Sbjct: 181 HFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRI 300
           LQL+WS  A+DI  G L ++ITDPS+R C+  ILKPD   A+FI  EC  +NWE I+TRI
Sbjct: 241 LQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRI 300

Query: 301 WPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTI 360
           WP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSECYFGLNLNPM KPSEVSYTI
Sbjct: 301 WPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTI 360

Query: 361 MPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGF 420
           MPNMAYFEF+P           K V+LVDV +GKEYELV+TTYAGL RYRVGDILRVTGF
Sbjct: 361 MPNMAYFEFIPL-------GGTKAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGF 420

Query: 421 HNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLL-KEVKTSVVEYTSYADTTTI 480
           HN+APQFHFVRRKNV+LSIDSDKTDE+ELQKAVENAS +L +E  + V EYTSYADT+TI
Sbjct: 421 HNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTI 480

Query: 481 PGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKS 540
           PGHYV+YWEL+V++   + PS ET+ +CCL MEESLNSVYRQ RVADNS+G LEIRVV++
Sbjct: 481 PGHYVLYWELLVRD-GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRN 540

Query: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           GTFEELMDYAISRGASINQYKVPRCVNFTPI+ELLDSRVVSAHFSP+LP WTP RRR
Sbjct: 541 GTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPERRR 589

BLAST of CmoCh04G004950 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 922.5 bits (2383), Expect = 1.7e-268
Identity = 463/605 (76.53%), Postives = 521/605 (86.12%), Query Frame = 0

Query: 1   MAVDSSLSSPL-GPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFL 60
           MAVDS L S +      EKD KAL+FIE+MTRN D+VQ  VL EILTRN++TEYL+R+ L
Sbjct: 1   MAVDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDL 60

Query: 61  AGATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
            G  D  TFK K+PV+TYEDL+PEIQRI++GD SPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  DGVVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+LDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSE++T GGL ARPVLTSYYKS
Sbjct: 121 TIEEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKS 180

Query: 181 EHFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIR 240
           +HFK RPYDPY VYTSPNEAILC DS QSMY QMLCGLL RH+VLRLGAVFASGLLRAI 
Sbjct: 181 DHFKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAIS 240

Query: 241 FLQLNWSDLANDIRTGKLSTKITDPSLRDCIEKIL-KPDEESADFISEECSKENWEGIVT 300
           FLQ NW +LA DI TG LS++I DP++++ + KIL KPD+E A+F+   CS+ENWEGI+T
Sbjct: 241 FLQNNWKELARDISTGTLSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIPTL+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDSAPK-------LVDLVDVEVGKEYELVITTYAGLYRYRV 420
           TIMPNMAYFEFLPH  N   D A +       LV+L +VEVGKEYELVITTYAGLYRYRV
Sbjct: 361 TIMPNMAYFEFLPH--NHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRV 420

Query: 421 GDILRVTGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYT 480
           GDILRVTGFHN+APQF F+RRKNV+LS++SDKTDEAELQKAVENAS+L  E  T V+EYT
Sbjct: 421 GDILRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYT 480

Query: 481 SYADTTTIPGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGA 540
           SYA+T TIPGHYVIYWEL+ ++      S+E M +CCL MEESLNSVYRQ RVAD SIG 
Sbjct: 481 SYAETKTIPGHYVIYWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGP 540

Query: 541 LEIRVVKSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWT 597
           LEIRVV++GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVSAHFSP+LP W+
Sbjct: 541 LEIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWS 600

BLAST of CmoCh04G004950 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-264
Identity = 458/598 (76.59%), Postives = 516/598 (86.29%), Query Frame = 0

Query: 1   MAVDSSLSSPLGPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLA 60
           M VDS+L SP+      KD KAL+FIE+MTRN D VQ+ V+ EIL+RN+ TEYL+R+ L 
Sbjct: 1   MTVDSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLK 60

Query: 61  GATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPT 120
           G TD  TFK K+PV+ Y+DL+PEIQRIA+GDRS ILSS+PI+EFLTSSGTSAGERKLMPT
Sbjct: 61  GFTDRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPT 120

Query: 121 IKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSE 180
           I E++DRRQLLYSLLMPVMNLYVPGLDKGK LYFLFVK+E++TPGGL ARPVLTSYYKSE
Sbjct: 121 IDEDMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSE 180

Query: 181 HFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRF 240
            FK RP DPY VYTSPNEAILCPDS QSMYTQMLCGLL RH+VLRLGAVFASGLLRAI F
Sbjct: 181 QFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGF 240

Query: 241 LQLNWSDLANDIRTGKLSTKITDPSLRDCIEKIL-KPDEESADFISEECSKEN-WEGIVT 300
           LQ NW +LA+DI TG LS++I+DP++++ + KIL KPD+E ADFI+  C ++N WEGI+T
Sbjct: 241 LQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMAQYIP L+YYSGGLP+ACTMYASSE YFG+NL PMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPHEPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVT 420
           TIMPNMAYFEFLPH       S  +LV+L DVEVGKEYELVITTYAGL RYRVGDIL+VT
Sbjct: 361 TIMPNMAYFEFLPHHEVPTEKS--ELVELADVEVGKEYELVITTYAGLNRYRVGDILQVT 420

Query: 421 GFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTT 480
           GF+N+APQF FVRRKNV+LSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+T T
Sbjct: 421 GFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKT 480

Query: 481 IPGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVK 540
           IPGHYVIYWEL+VK+     P+ E M +CCL MEESLNSVYRQ RVAD SIG LEIRVVK
Sbjct: 481 IPGHYVIYWELLVKDQTNP-PNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVK 540

Query: 541 SGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           +GTFEELMDYAISRGASINQYKVPRCV+FTPIMELLDSRVVS HFSPALP W+P RRR
Sbjct: 541 NGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPERRR 595

BLAST of CmoCh04G004950 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 859.4 bits (2219), Expect = 1.7e-249
Identity = 432/598 (72.24%), Postives = 503/598 (84.11%), Query Frame = 0

Query: 1   MAVDSSLSSPL-GPPACEKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFL 60
           MAVDS L S +  P   E + KAL+FIE++TRN D+VQ  VL EIL+RN++TEYL+R+ L
Sbjct: 1   MAVDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDL 60

Query: 61  AGATDPNTFKDKLPVITYEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMP 120
            GA D  +FK K+PV+ YEDL+ +IQRI++GDRSPILSSHPI+EFLTSSGTSAGERKLMP
Sbjct: 61  NGAVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMP 120

Query: 121 TIKEELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKS 180
           TI+E+++RRQLL +LLMPVMNLYVPGLDKGKGLYFLFVKSE+ T GGL ARP LTSYYKS
Sbjct: 121 TIEEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKS 180

Query: 181 EHFKTRPYDPYMVYTSPNEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIR 240
           ++F+T   D   VYTSP EAILC DS QSMYTQMLCGLL RH+V RLGAVF SGLLRAI 
Sbjct: 181 DYFRTSDSD--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAIS 240

Query: 241 FLQLNWSDLANDIRTGKLSTKITDPSLRDCIEKIL-KPDEESADFISEECSKENWEGIVT 300
           FLQ NW +L+ DI TG LS+KI D +++  +  IL KPD+E A+F+   CS+ENWEGI+T
Sbjct: 241 FLQNNWKELSQDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIIT 300

Query: 301 RIWPKTKYLDVIVTGAMAQYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSY 360
           +IWP TKYLDVIVTGAMA+YIP L+YYSGGLP+A  +YASSE YFG+NLNPMCKPSEVSY
Sbjct: 301 KIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSY 360

Query: 361 TIMPNMAYFEFLPH-EPNSGNDSAPKLVDLVDVEVGKEYELVITTYAGLYRYRVGDILRV 420
           TI PNMAYFEFLPH     G   A  LV+L DVEVGKEYELVITTYAGLYRYRVGDILRV
Sbjct: 361 TIFPNMAYFEFLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRV 420

Query: 421 TGFHNAAPQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTT 480
           TGFHN+APQF F+RR+NV+LSI+SDKTDEA+LQKAVENAS+LL E  T V+EYTSYADT 
Sbjct: 421 TGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTK 480

Query: 481 TIPGHYVIYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVV 540
           TIPGHYVIYWEL+ ++     PS E M +CCL MEESLN+VYRQ RV+D SIG LEIRVV
Sbjct: 481 TIPGHYVIYWELLSRDQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVV 540

Query: 541 KSGTFEELMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARR 596
           ++GTFEELMD++ISRG+SINQYKVPRCV+ TPIM+LLDSRVVSAHFSP+LP W+P RR
Sbjct: 541 QNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHWSPERR 596

BLAST of CmoCh04G004950 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 822.4 bits (2123), Expect = 2.4e-238
Identity = 404/592 (68.24%), Postives = 481/592 (81.25%), Query Frame = 0

Query: 17  EKDAKALQFIEQMTRNADAVQRTVLAEILTRNASTEYLRRYFLAGATDPNTFKDKLPVIT 76
           EK+   LQFIE +T NAD VQR VL EIL+RNA  EYL+R+ L G TD  TFK  +PV+T
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 77  YEDLQPEIQRIASGDRSPILSSHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136
           YED+QPEI RIA+GD+S +L S+PISEFLTSSGTS GERKLMPTI+EELDRR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 137 PVMNLYVPGLDKGKGLYFLFVKSETRTPGGLLARPVLTSYYKSEHFKTRPYDPYMVYTSP 196
           PVM+ +VPGLDKGKG+YFLF+KSE++TPGGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 197 NEAILCPDSFQSMYTQMLCGLLERHQVLRLGAVFASGLLRAIRFLQLNWSDLANDIRTGK 256
           N+ ILC DS+QSMY+QMLCGL +  +VLR+GAVFASG +RAI+FL+ +W +LA DIRTG 
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 257 LSTKITDPSLRDCIEKILKPDEESADFISEECSKENWEGIVTRIWPKTKYLDVIVTGAMA 316
           LS++ITD S+R+ + +ILKPD + ADF+  EC K +W+GI+TR+WP TKY+DVIVTG M+
Sbjct: 259 LSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMS 318

Query: 317 QYIPTLDYYSGGLPLACTMYASSECYFGLNLNPMCKPSEVSYTIMPNMAYFEFLPHEPNS 376
           QYIPTLDYYS GLPL CTMYASSECYFG+NL P+CKPSEVSYT++PNMAYFEFLP   NS
Sbjct: 319 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNS 378

Query: 377 G---NDSAPK---------LVDLVDVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNAA 436
           G   + S PK         LVDLVDV++G+EYELV+TTYAGLYRYRVGD+L V GF N A
Sbjct: 379 GVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNA 438

Query: 437 PQFHFVRRKNVVLSIDSDKTDEAELQKAVENASKLLKEVKTSVVEYTSYADTTTIPGHYV 496
           PQF F+ RKNVVLSIDSDKTDE ELQ AV+NA   L     S+ EYTSYADT++IPGHYV
Sbjct: 439 PQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYV 498

Query: 497 IYWELMVKETEGKWPSKETMEQCCLAMEESLNSVYRQGRVADNSIGALEIRVVKSGTFEE 556
           ++WEL +       PS    E CCL +EESLNSVYRQGRV+D SIG LEI++V+SGTF++
Sbjct: 499 LFWELCLNGNTPIPPS--VFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDK 558

Query: 557 LMDYAISRGASINQYKVPRCVNFTPIMELLDSRVVSAHFSPALPRWTPARRR 597
           LMDYAIS GASINQYK PRCV F PI+ELL+SRVV ++FSP  P+W+P  ++
Sbjct: 559 LMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQ 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823334.7e-28481.24Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221902.7e-26376.59Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS01.8e-25473.99Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z61.8e-25473.99Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M23.7e-25272.64Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
A0A6J1FTR90.0e+00100.00probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1IUJ60.0e+0099.50probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1E8A00.0e+0093.79probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1J7V10.0e+0093.62probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
A0A5A7T1480.0e+0091.00Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
XP_022943254.10.0e+00100.00probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata][more]
XP_023519906.10.0e+0099.66probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
XP_022979865.10.0e+0099.50probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita maxima][more]
XP_022922958.10.0e+0093.79probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita moschata][more]
XP_023552180.10.0e+0093.62probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
AT2G14960.13.4e-28581.24Auxin-responsive GH3 family protein [more]
AT4G37390.11.7e-26876.53Auxin-responsive GH3 family protein [more]
AT2G23170.11.9e-26476.59Auxin-responsive GH3 family protein [more]
AT1G59500.11.7e-24972.24Auxin-responsive GH3 family protein [more]
AT5G54510.12.4e-23868.24Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 446..466
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 5..596
IPR004993GH3 familyPFAMPF03321GH3coord: 26..567
e-value: 3.2E-206
score: 686.3
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 5..596

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G004950.1CmoCh04G004950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity