CmoCh04G004090 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G004090
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionERAD-associated E3 ubiquitin-protein ligase HRD1B-like
LocationCmo_Chr04: 2024454 .. 2030582 (+)
RNA-Seq ExpressionCmoCh04G004090
SyntenyCmoCh04G004090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCATCTTGGTTCTGCATCAGCTTTCATGATTCTGCAATTTTTCGTGTTCGTCTCATCTTCATCGACCTCCAACTGGATTGCCGCCTAGATCTACCCGGGCATAGATAGCCATAGTCGCCGATCAAACAGGCAAGATTTCAATTCGAAAAACAACTTTTTTTTTGTTGATGTTTGTGGATTTGCTCGGTCAATTGGGTTGTAACACCAAGATTACGGGTTTCTGGTTTACCAAGTTTAGTCAGACCATGTTGCAAATACATTACATTGTCCGCGTCGAGACAGAACAAATGTTAGCGCTGGAAAAATTGTAGGAAAATGCCTCTCCCGGCTGGTGTTATTGAATGTAGGGATGGTATTGATGAACTCAACATAGATCATAGGGAATTAATTCACTTCCGCAAACCTTAGGATGATTAATCTCAGTCGAATTTGCATGCATTTGTCGCGTGGATAAGACAAAACAGCGTGTATTTTGCCTGATTTTCTAATGATCAGTGCTTTTGGGCTTTGAGTGGGAATTGGGACCTGGAACTTTTTGGCATTATGTAAACGGAGGGAACCGGGTTGCTAAAATGGGTACATCTCATTCGTAAAGCTTCCTATATTTTATAGTTAAACTCATAGAGCTTTGACCTAGAGCTTGGGTTGGCCTCCATCTCCCTTTTTATTGGCCATCTTTTGGAAAATTCGATGGTGTATTAGAAATCTGAACATCTTAATTTATTAACTTCACATTGCCAAATCAATTATAATTCTCTTTTTGGGGAACCTAAGGGAGCCTTTGAAGCATTTAAAATTGAATTTTTCCCTCAAGCTACGAGGATCTCCCAGGAAGCATTGCATAAATATGCACACGATATGGGGGATGCAGAGGTCTCAAGTTCACTTATCTTCAATAATCTAGAAAGAGATTACATGAAGAGTTATCAAATATTGGAATTAAGCATAAAATGGACGTCACTCTAGTTTTTAGACCAGGACGTCCATGAGAAACCAGAAAATGAAGTTTCCTATAGAAGATCTTCATCATTGAAAATTCCCCTATTTCCCCCAGCCAACCCGGAAAAGCTCGGATTGCTTGGACAAGGAAAATCCTCACATTCATGAAAGGCTTTTTATGCATTACTTCTTAATTTAAATGTCGTCCATACAGATAATCAATTTACTATAAAGATAGGCAACAATGATATTTTAGCATCGATATGTTGTTTCATTTGGCTGACATTTCTAATTCTCACATGGCAGTGCAGTAGAGAATGATGAAGCTGCAAACATATGCGGGAATTAGTTTCATTGCAACTATAGTGGTGATATATCATGCCTTTAACAGTAGAGGCCAGTTCTACCCAGCGTTGGTCTATTTATCAACTTCCAAGATCAATCTAGTGCTTCTTCTCAACATGGGTTTAGTCCTCATGTGCATTTTGTGGCAGCTAACCAAAAAAATATTTCTTGGTTCTCTCCGAGAAGCAGAGGTTGAGAGGCTCAATGAGCAATCGTGGAAGGAATTGATGGAAATCCTCTTTGCCATCACTATCTTTCGGCAAGATTTTTCTGTTGCATTCCTTGCTATGGTTACGGCTTTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTGGGTTTTCTCTTTGTTCTAGATAGTCTGTTTTTGTATAATTCGATAGGTTCTCTGATACAGACAAGGAAGGCTTCAGTTTCCCTCTTCTTTTCATTTGAGTAAGTTGGCAATTTCAACCGTATGGTATATAACTTGCATCTGTCTTTGTACAAGCATAAACTATGATGTTTTGTGGTTAGAATGTCAAATATTTCAAGGCCATGTTAGAAGAACTAACAAATTATTTTTAAATTGGATATTTAATATCGTATATAGAGAATTCATGACTACTGTCTTTTTGTTTTTTTCTTGCTGGAGTTAGATCTTTTAAATATCCTATGATGTTTGTTTCTGGATCTCTGAGGATATTTTGTCATCATTAAATAAAGTCAAGGACTTGCTTTTCAGGTATATGATTCTTGCGACAACAGCCGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAAAAAGCCTGTCTATACATTCTACTTGGAGCTTATCCGAGACTTGCTTCACTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGTAAGTAATCAAGCTACATTTCCTAAGCCTATCTCATTTGTGTGAATTGGAAGTTTGAATTAATCCCTTTCTGTAAAGATTTACTCATATTGCTTAAGCCCAAATGTCAAAGTAGGAGGATGCTGGTTTTTCTCAAAGAATATTATGCAAACTATAATAATCATGTGGTAACTGGGATTTGTGCCCTTAATATTGGCTGTCAGATTTTACTTTCATGTTAATTATGAGAGATGGCTCACAGGAGTGTCTTATGACTGACATGGTATTTGTTGATTGTTTTGATATCCTTTACATCCTTATTATTTTCAATCTGTTGTTCTTATTTCCCTTTTAGATAGAAAGAGGCAGAGCTTTCTGCTTTCATGCACACATTGCCACTAGAAAAAAGATGCTTTTGATTGTAGTCTTACGAATTGTTTTTTTTTGGAGTTTTTTGGATAAGAGCTCTGTGGTCATGATTTCATTGATAGACTGTAAACACAGAAAGATATTCATTTCACTAACACGGTTCCGTTTTGGACTTTCAACTATCTGTTCTCTCCTTTCTACTATATTGAATGCCAACTGCTTAACACATTTAACGATATAAGGAGGTTAAATGAAGCTCCAGATTAAGTGTATTATGCAAATTTCTTGACTTAAATATGCTGGTATTTAATATACGTTTAATAGTTTAAAATGCACGGTTTTATATCCTCTATTATTCTATCTTCACTGTATTTGCTCTTTATTAACTTCATTCTCTTTTCTCATTTTAATAATGCTGAGTTTGCTCTTGTAGGACATATGGCGTGCCTTTGCACTTGATCCGTGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATTCGGTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACACCTGAAGAACTCAATTCGTGCGTCTAAGCTTGGTCAACTTATTATGTCTATTATGTTTCCAATATTTATATTTCAGCTACTAAAATTCATTTTACGGAATTGCAGAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGGTCCTGGTTGGAGCGACAACATACTTGTCCCACTTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGGAGGTCATCAGGGATTGCAGTCTGATGCTAACCTACAAGGTAATTGTGAAGAATTTAGTTTAATTTACTGCTTGGCTAAAAGATCTTAGATCTTGTGCAATTTTTAATTTGATAAATTTGTTCAATCTCTTCAATCACCATGAATTGACCAAGCCTTTCAATATGTACTTGGAGGGAATAGACTCAAGTCATGGTGGCCACCAACCTAAGTTTTAATGCTCACCAGTTTCTTAGGAAACCGAAGGTAGTAGGTCAGACTATTGTCTCGTGAGATTAGTCAGTTAGTGTGTGCAAGTTGGTTTGGACAATCGGGATATCAAAAAAAAAAAAAAAAAAAACTGATCTCTTCCATTTATATATTTCATCTAGTGATGGATTTGTTTGGAATCTTTCGTTTTCCTCAAAATTTCAACTAAGGAAGAAAAGCATGAAAGATCTGGAAGACACTTTTTCTTGAAGTATCAAAGCTAAAAGAGTACTCTTTGTTATTGCTTATGTTACATTCAGATACCTAGAGACATATCTGTCCCATCTGCCACGTCACCTACAAATTAGATAGTGCACAAATTTTCTGGTATTTTACTTGTACAGTGCTTCCCTGCATCTCAAATCTCTTAACGAGTGGTGCTTTTTATACAGCATCCACAACCCAATTTGGTTCAACGGTTCAAAACTATTAAATAATCCGGGTCATTAAGTAACAATGCAAAAATGAGCCATCAGTATTTGCAAGTCTTCTCTATACGTAGTATATACCTCACCATCAGGGTGAAAGAGTTGAAGTTTTGGTCTCTCACAATTCATTTCATGGGTGTTGAATTTCTTTCTTCTCTCATCTTTTTTCTTATTTTTTTTTGAATTCATGCATTATATTCGTGCTTGTTTTTATTAAGAGACTGTCCTTAAAGCTATCAAATATTCTATATATTTTTGTGTCCTAGCAATTCAAACTCGTCTAAAGGAAGTAGTTTGATTCTAGGAATGTCTACGAATTTAAGCACATCTCTTTGAGAACAGATGATCATTCTCGAACTAGACCTCTGGTTCTCTGTAAAAATTGGGTGCTTCATTTTATCTTTTCTTGTTTTGTTTTTAATTTTCTTATTGTCAGAAATGCTGTGGGTGTATTTCCAAGTTTTCCATTGAAATTCTGAAAAGCCTTTTCTCCACCTAAATCACTGTTTGGAGTCTTTTACTCGATCTCATTGCTTTGGATTAACTGCATTAACTGCATTAACTGCTTGATTGTTTGGTGCATGCCAGTAGAGCATTCTTTTCAGTGTATCTGCTTTCTTTGATAGAAGTTTTGGATGCGAATTATGGAACTGTTGGTTTCATTTAATTATTTTTTCAATATGGTAGTCTACCAGGCTGAAACTTGGATGTAATGACTGACCATTCTGTTGGAAACAGGAACAGGAACCACAAGTTCGTCTGCACAAGGTTCGGGTGGTACAAGTGCTGCGGGTGATAGTTTGGGTCATCATCAGGCTAGAGTCCAGGCTGCTGCTGCTGCTGCTTCAATATATGAGAAATCTTATGTTTATCCTTTTGGGAACACATTAGCATGGTATAGCTTTTTATTGCAAAATATGTGGTCCCTGTCTAATTCTCCTTTTTTTGGTCTTGAAAATTTACTTCAATATTAAGGCCATTTCTCCCCTTGTATATAACAGCCCTCCACCCCAAAGAGAGAGAAAAGAGTATGACATATATATATTATATAATTTATATTTTGTTTAATTTTATTTATTTCTAGGTCTCCTGGATATGCAGTAATTCCTCAGGTTCAAAGACCTTTGCTTGACTCCAACAATGTAGAACCGATTGGAGAACAAGCTCCCAATGAACAAGCACAATATTTTCCATTTACAGGCGGCCCTGCAAACCTGTCCTTTACTCAAATCCCCCAAAATCTTTTTATTCCTTTTCAGCCACGAGAGGGCACTGTAAACAATACGGATGGATTGGGCAGTAGTCAAAATATATCAGGCTCACAGCTTGAATCTCAAGAAAGATTTCTTCAACAACAGATTGAGGTATAATATTTAATTATAGCACGAACATTACCTGGTGAACTGTCTTGTTTAGTTAGTGTAGGTTCAGTTTTGGCTCTTGTTGCTGGACCCTTGTATATTCTTTAGCTCAAGGTGAGGTGATTCAATTAGAGGCTGAGAGGAGCTTGCTTGTATTTTGAAGGAAGAAATAAATAGTTTATCTTGTTTTTAGATCTTTCATTGAGAAAGCGTAAAGTTCTTCACACTGCTTCCAAACAACTCTATATTTTATAGAAACTCGAGGGTAGATAGAAGTGAAGAGGAGTCATAAAATCAAATTGTTAACAAGGAGTATGTGATAAGTGAAGGATATATACCCCGTAAAGAAATAAATTTGTATTTATTGAATTCCATTCACACTAATTTGACCGAGGCTGAGGGTCTTTCTTTAGGAGAGTCCACTTTTTTTTGGCTGAATGACCACTTCCTCTAATATGCAAATTTTCATTTTTGCCAACAGATCCTACAAGATCAGCTGCAACTTCTCCGTAAACTGAAAGTCGAGGAAAGCTCAATAGCAGGCGAGGCAGACAAGAAAGGGAAAAGCATAGCATCATCGTCGCAGTCATCATCTTCATCAGTTTCTGATTCTCATCCACACGGAGAAACGGAAAGGACGGTGGGATAA

mRNA sequence

ATTCCATCTTGGTTCTGCATCAGCTTTCATGATTCTGCAATTTTTCGTGTTCGTCTCATCTTCATCGACCTCCAACTGGATTGCCGCCTAGATCTACCCGGGCATAGATAGCCATAGTCGCCGATCAAACAGTGCAGTAGAGAATGATGAAGCTGCAAACATATGCGGGAATTAGTTTCATTGCAACTATAGTGGTGATATATCATGCCTTTAACAGTAGAGGCCAGTTCTACCCAGCGTTGGTCTATTTATCAACTTCCAAGATCAATCTAGTGCTTCTTCTCAACATGGGTTTAGTCCTCATGTGCATTTTGTGGCAGCTAACCAAAAAAATATTTCTTGGTTCTCTCCGAGAAGCAGAGGTTGAGAGGCTCAATGAGCAATCGTGGAAGGAATTGATGGAAATCCTCTTTGCCATCACTATCTTTCGGCAAGATTTTTCTGTTGCATTCCTTGCTATGGTTACGGCTTTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTGGGTTTTCTCTTTGTTCTAGATAGTCTGTTTTTGTATAATTCGATAGGTTCTCTGATACAGACAAGGAAGGCTTCAGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCGACAACAGCCGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAAAAAGCCTGTCTATACATTCTACTTGGAGCTTATCCGAGACTTGCTTCACTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGACATATGGCGTGCCTTTGCACTTGATCCGTGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATTCGGTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACACCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGGTCCTGGTTGGAGCGACAACATACTTGTCCCACTTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGGAGGTCATCAGGGATTGCAGTCTGATGCTAACCTACAAGGAACAGGAACCACAAGTTCGTCTGCACAAGGTTCGGGTGGTACAAGTGCTGCGGGTGATAGTTTGGGTCATCATCAGGCTAGAGTCCAGGCTGCTGCTGCTGCTGCTTCAATATATGAGAAATCTTATGTTTATCCTTTTGGGAACACATTAGCATGGTATAGCTTTTTATTGCAAAATATGTGGTCCCTGTCTCCTGGATATGCAGTAATTCCTCAGGTTCAAAGACCTTTGCTTGACTCCAACAATGTAGAACCGATTGGAGAACAAGCTCCCAATGAACAAGCACAATATTTTCCATTTACAGGCGGCCCTGCAAACCTGTCCTTTACTCAAATCCCCCAAAATCTTTTTATTCCTTTTCAGCCACGAGAGGGCACTGTAAACAATACGGATGGATTGGGCAGTAGTCAAAATATATCAGGCTCACAGCTTGAATCTCAAGAAAGATTTCTTCAACAACAGATTGAGATCCTACAAGATCAGCTGCAACTTCTCCGTAAACTGAAAGTCGAGGAAAGCTCAATAGCAGGCGAGGCAGACAAGAAAGGGAAAAGCATAGCATCATCGTCGCAGTCATCATCTTCATCAGTTTCTGATTCTCATCCACACGGAGAAACGGAAAGGACGGTGGGATAA

Coding sequence (CDS)

ATGATGAAGCTGCAAACATATGCGGGAATTAGTTTCATTGCAACTATAGTGGTGATATATCATGCCTTTAACAGTAGAGGCCAGTTCTACCCAGCGTTGGTCTATTTATCAACTTCCAAGATCAATCTAGTGCTTCTTCTCAACATGGGTTTAGTCCTCATGTGCATTTTGTGGCAGCTAACCAAAAAAATATTTCTTGGTTCTCTCCGAGAAGCAGAGGTTGAGAGGCTCAATGAGCAATCGTGGAAGGAATTGATGGAAATCCTCTTTGCCATCACTATCTTTCGGCAAGATTTTTCTGTTGCATTCCTTGCTATGGTTACGGCTTTGTTGTTGATCAAAGCTTTGCATTGGTTGGCTCAGAAGAGGGTTGAATACATGGAAACCACTCCATCGGTGCCGTTGTTGTCTCATATCCGGATTGTTTCTTTCTTGGGTTTTCTCTTTGTTCTAGATAGTCTGTTTTTGTATAATTCGATAGGTTCTCTGATACAGACAAGGAAGGCTTCAGTTTCCCTCTTCTTTTCATTTGAGTATATGATTCTTGCGACAACAGCCGTGTCAACATTTGTGAAATATATTTTCTATGTCAGTGATGTGCTCATGGAGGGACAATGGGAAAAAAAGCCTGTCTATACATTCTACTTGGAGCTTATCCGAGACTTGCTTCACTTGTCGATGTACTTGTGTTTCTTTCTTGTGATTTTCATGACATATGGCGTGCCTTTGCACTTGATCCGTGAGCTCTATGAGATCTTTAGGAATTTTAGGATTCGTATTGCAGATTACATTCGGTATAGAAAGATTACTTCAAACATGAATGATCGTTTTCCAGATGCAACACCTGAAGAACTCAATTCAAGTGATGCAACATGCATTATTTGTCGTGAAGAGATGACAGTTGCAAAGAAACTAGTATGTGGACATCTTTTTCATGTCCATTGCCTCCGGTCCTGGTTGGAGCGACAACATACTTGTCCCACTTGCAGAGCCCTTGTTGTACCACCTGAAGGTTCAAGCACAGCAGGAGGTCATCAGGGATTGCAGTCTGATGCTAACCTACAAGGAACAGGAACCACAAGTTCGTCTGCACAAGGTTCGGGTGGTACAAGTGCTGCGGGTGATAGTTTGGGTCATCATCAGGCTAGAGTCCAGGCTGCTGCTGCTGCTGCTTCAATATATGAGAAATCTTATGTTTATCCTTTTGGGAACACATTAGCATGGTATAGCTTTTTATTGCAAAATATGTGGTCCCTGTCTCCTGGATATGCAGTAATTCCTCAGGTTCAAAGACCTTTGCTTGACTCCAACAATGTAGAACCGATTGGAGAACAAGCTCCCAATGAACAAGCACAATATTTTCCATTTACAGGCGGCCCTGCAAACCTGTCCTTTACTCAAATCCCCCAAAATCTTTTTATTCCTTTTCAGCCACGAGAGGGCACTGTAAACAATACGGATGGATTGGGCAGTAGTCAAAATATATCAGGCTCACAGCTTGAATCTCAAGAAAGATTTCTTCAACAACAGATTGAGATCCTACAAGATCAGCTGCAACTTCTCCGTAAACTGAAAGTCGAGGAAAGCTCAATAGCAGGCGAGGCAGACAAGAAAGGGAAAAGCATAGCATCATCGTCGCAGTCATCATCTTCATCAGTTTCTGATTCTCATCCACACGGAGAAACGGAAAGGACGGTGGGATAA

Protein sequence

MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQLTKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLAQKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYMILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYGVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMTVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTTSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPREGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGKSIASSSQSSSSSVSDSHPHGETERTVG
Homology
BLAST of CmoCh04G004090 vs. ExPASy Swiss-Prot
Match: Q9LW77 (ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=3702 GN=HRD1A PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.3e-169
Identity = 343/555 (61.80%), Postives = 403/555 (72.61%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LVLM  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF+Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT V+ FVKY+FYV+D+LM+GQWEKKPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGG-----HQGLQSDANLQ 360
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++A       HQG Q      
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ----- 360

Query: 361 GTGTTSSSAQGSGGTSAAG---DSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFL 420
             GT+SS  QGS  +S+AG   +SL  H AR+QAAA+AAS+Y KS VYP  NT+AW S +
Sbjct: 361 --GTSSSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSGV 420

Query: 421 LQNMWSLSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNL 480
                                       P  EQ   E  Q  P    P   S        
Sbjct: 421 ----------------------------PGTEQVSTEPDQTLPQHNLPVENSH------- 480

Query: 481 FIPFQPREGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIA 540
                             +  N+S ++LE   + L+  +EIL+++L  L   K E    A
Sbjct: 481 ------------------AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPES---A 492

Query: 541 GEADKKGKSIASSSQ 548
           GE + KGKS+A +++
Sbjct: 541 GEPENKGKSVADAAE 492

BLAST of CmoCh04G004090 vs. ExPASy Swiss-Prot
Match: Q6NPT7 (ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=3702 GN=HRD1B PE=2 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 7.7e-167
Identity = 332/548 (60.58%), Postives = 395/548 (72.08%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLVLM  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+++V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI  LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANLQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA G++G   ++  QGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYP-FGNTLAWYSFLLQNMWS 420
           +SS  QGS  ++AA +++  H+AR QAAA+AASIY +S VYP   NTL W          
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVW---------- 420

Query: 421 LSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQP 480
            S G +++PQ                                                  
Sbjct: 421 -SQGNSLLPQ-------------------------------------------------- 460

Query: 481 REGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKK 540
                              +++E+Q RFL+ QIE+L +QL+LL K            D K
Sbjct: 481 -------------------TEVEAQRRFLESQIEVLTNQLRLLEK--------PTTVDTK 460

Query: 541 GKSIASSS 547
           GKS+A ++
Sbjct: 541 GKSVADTA 460

BLAST of CmoCh04G004090 vs. ExPASy Swiss-Prot
Match: Q5Z880 (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica OX=39947 GN=HRD1 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-159
Identity = 313/526 (59.51%), Postives = 387/526 (73.57%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++LQTYA  S +AT   +Y+AF+SR QFYPA+VYLSTSKI  VLLLN GLV MC+ WQL
Sbjct: 1   MIRLQTYAAFSLMATATAVYYAFSSREQFYPAMVYLSTSKICFVLLLNTGLVAMCVAWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            K++FLG+LREAEVERLNEQ+W+E++EILFA+TIFRQDFSV+FLAMV ALLL+KALHWLA
Sbjct: 61  VKRLFLGTLREAEVERLNEQAWREVVEILFAVTIFRQDFSVSFLAMVAALLLVKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSVP+LSH RIVSF+ FL V+D LFL NS+ SLI  R+ASV++FFSFEYM
Sbjct: 121 QKRVEYIETTPSVPMLSHARIVSFMLFLLVVDCLFLSNSLRSLIHKREASVAIFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILAT+ VSTFVKYIFYVSD+LMEGQWEKK VYTFYLELI DL+HLS+Y+ FF+ IF+ YG
Sbjct: 181 ILATSTVSTFVKYIFYVSDMLMEGQWEKKAVYTFYLELISDLVHLSLYMLFFIAIFLNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYE FRNFRIRIADY+RYRKITSNMN+RFPDAT +ELN+SDATCIICREEMT
Sbjct: 241 VPLHLIRELYETFRNFRIRIADYVRYRKITSNMNERFPDATADELNASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTA----GGHQGLQSDANLQG 360
            AKKL+CGHLFHVHCLRSWLERQHTCPTCRA ++PP+   TA    G H G+Q    + G
Sbjct: 301 TAKKLLCGHLFHVHCLRSWLERQHTCPTCRAPILPPDNGRTAARPHGVHPGVQP---VPG 360

Query: 361 TGTTSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNM 420
            GT        G   AAG+++   QA+++AAA+AAS+Y +S+ YP  N L  YS      
Sbjct: 361 NGT-------PGSERAAGENISRRQAKLEAAASAASLYGRSFAYPPANNLNRYS------ 420

Query: 421 WSLSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPF 480
                          P   ++N    GE + + Q+     T  P+  +F           
Sbjct: 421 --------------TPPQSTSNGPQSGEASTSNQSPKGHATADPSAPTFY---------- 480

Query: 481 QPREGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLR 523
               G V++   + +++ +  S  ++ E  ++ QIE+LQ QLQ+ +
Sbjct: 481 --ARGAVSS---VTTTRELESSLQKAYENAIKSQIEMLQIQLQMFQ 481

BLAST of CmoCh04G004090 vs. ExPASy Swiss-Prot
Match: Q803I8 (E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2)

HSP 1 Score: 264.6 bits (675), Expect = 2.5e-69
Identity = 143/331 (43.20%), Postives = 203/331 (61.33%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     VL+ +L +L
Sbjct: 1   MVRAALVTATSLALTGAVVAHAYFLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            +K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+A+ T LL +K  HWLA
Sbjct: 61  MRKVFFGQLRAAEMEHLIERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           + RV++ME +P++  + H R++S +  L V+D LF+ ++  S+I TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWVFHFRVLSLMVLLGVMDFLFVNHACHSII-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++TF+KY  +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTTFIKYTLHTIDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPE+L ++D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEDLQATDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
           T AKKL C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKKLPCNHIFHSSCLRSWFQRQQTCPTCR 330

BLAST of CmoCh04G004090 vs. ExPASy Swiss-Prot
Match: Q86TM6 (E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-68
Identity = 143/331 (43.20%), Postives = 200/331 (60.42%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M +       S   T  V+ HA+  + QFYP +VYL+ S  ++ +L     VL+ +L ++
Sbjct: 1   MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKV 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
             K+F G LR AE+E L E+SW  + E   A T+FR DFS  F+A+ T LL +K  HWLA
Sbjct: 61  MGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           + RV++ME +P++  L H RIVS +  L +LD LF+ ++  S++ TR ASV L F FEY 
Sbjct: 121 EDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSIL-TRGASVQLVFGFEYA 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           IL T  ++ F+KY+ +  D+  E  W+ K VY  Y EL    + + +Y+ F  ++   + 
Sbjct: 181 ILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHT 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM- 300
            PL  IR +Y   R F+  + D I  R+   NMN  +PDATPEEL + D  CIICREEM 
Sbjct: 241 FPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMV 300

Query: 301 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCR 331
           T AK+L C H+FH  CLRSW +RQ TCPTCR
Sbjct: 301 TGAKRLPCNHIFHTSCLRSWFQRQQTCPTCR 330

BLAST of CmoCh04G004090 vs. ExPASy TrEMBL
Match: A0A6J1FNJ3 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=3662 GN=LOC111447399 PE=4 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.1e-301
Identity = 556/567 (98.06%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE
Sbjct: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHPHGETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPHGETERTVG 556

BLAST of CmoCh04G004090 vs. ExPASy TrEMBL
Match: A0A6J1KWZ5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499418 PE=4 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.3e-294
Identity = 543/567 (95.77%), Postives = 548/567 (96.65%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSS QGSGGTSAAGDSL HHQ+RVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSEQGSGGTSAAGDSLSHHQSRVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQVQRPLLDSNNVEPIGEQA NEQAQYFPF GGPANLS+TQIPQNLF+PFQPRE
Sbjct: 421 PGYAVVPQVQRPLLDSNNVEPIGEQAANEQAQYFPFAGGPANLSYTQIPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGL S QNISGSQLE+QERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLSSGQNISGSQLEAQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHP GETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPPGETERTVG 556

BLAST of CmoCh04G004090 vs. ExPASy TrEMBL
Match: A0A6J1CJF5 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.3e-270
Identity = 504/567 (88.89%), Postives = 519/567 (91.53%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLF YNSI  LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGH G   DAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQ S GT  AGD+L HHQARVQAAAAAASIYEKSYVYP  +TL W           S
Sbjct: 361 SSSAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQ QRPL DSNN+EP GEQAPN Q QYFPFTGGPANLSFTQ PQNLF+PFQPRE
Sbjct: 421 PGYAVVPQTQRPLADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           G+VNNTDGLGSSQN+SG  LE QERF+QQQIE+LQ QLQLLR  KVEES+ AGEADKKGK
Sbjct: 481 GSVNNTDGLGSSQNVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTRAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSH-PHGETERTV 567
           S+ASS  SSSSSVS+SH P GE ER V
Sbjct: 541 SVASSPPSSSSSVSESHPPAGEIERMV 555

BLAST of CmoCh04G004090 vs. ExPASy TrEMBL
Match: A0A6J1CI55 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011717 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 6.1e-268
Identity = 502/567 (88.54%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFL FLF+LDSLF YNSI  LI+TRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLSFLFILDSLFFYNSIEYLIRTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGH G   DAN Q  GTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHHG-SHDANQQ--GTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQ S GT  AGD+L HHQARVQAAAAAASIYEKSYVYP  +TL W           S
Sbjct: 361 SSSAQDSSGTGVAGDNLSHHQARVQAAAAAASIYEKSYVYPCASTLVW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQ QRPL DSNN+EP GEQAPN Q QYFPFTGGPANLSFTQ PQNLF+PFQPRE
Sbjct: 421 PGYAVVPQTQRPLADSNNIEPNGEQAPNGQPQYFPFTGGPANLSFTQFPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           G+VNNTDGLGSSQN+SG  LE QERF+QQQIE+LQ QLQLLR  KVEES+ AGEADKKGK
Sbjct: 481 GSVNNTDGLGSSQNVSGLHLEDQERFIQQQIEMLQKQLQLLRNPKVEESTRAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSH-PHGETERTV 567
           S+ASS  SSSSSVS+SH P GE ER V
Sbjct: 541 SVASSPPSSSSSVSESHPPAGEIERMV 553

BLAST of CmoCh04G004090 vs. ExPASy TrEMBL
Match: A0A0A0LJG4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 PE=4 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 3.5e-263
Identity = 493/568 (86.80%), Postives = 514/568 (90.49%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATI+VIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLV+MCILWQL
Sbjct: 1   MMKLQTYAGISFIATIMVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVIMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFL MVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLGMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLF YNSI SLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFFYNSIDSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWE+KPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWERKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSST  GH G+QSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTTTGHHGVQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQG+ GT    D+L HHQARV+AAAAAASIYEKSYVYP  +TL W           S
Sbjct: 361 SSSAQGTSGTGVTNDNLSHHQARVRAAAAAASIYEKSYVYPCASTLVW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
            GYAV+PQV+RPL DSNN E  GEQ+PN   QYF F GGP+NLSFTQ PQN+F+PFQPRE
Sbjct: 421 LGYAVVPQVERPLFDSNNTETNGEQSPNGHPQYFSFPGGPSNLSFTQFPQNIFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSI-AGEADKKG 540
           GT NN + LGSS N+SGSQLE+QE+FLQQQIE LQ+QLQLLRK KVEESS  A EADKKG
Sbjct: 481 GTGNNIEELGSSLNVSGSQLEAQEKFLQQQIEFLQNQLQLLRKPKVEESSRGASEADKKG 540

Query: 541 KSIA-SSSQSSSSSVSDSHPHGETERTV 567
           KS+A S S S+S S SDSHP GETER V
Sbjct: 541 KSLAISPSSSASLSSSDSHPPGETERAV 557

BLAST of CmoCh04G004090 vs. NCBI nr
Match: XP_022942315.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_022942316.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata])

HSP 1 Score: 1045.0 bits (2701), Expect = 2.3e-301
Identity = 556/567 (98.06%), Postives = 556/567 (98.06%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE
Sbjct: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHPHGETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPHGETERTVG 556

BLAST of CmoCh04G004090 vs. NCBI nr
Match: KAG7030884.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1032.7 bits (2669), Expect = 1.2e-297
Identity = 550/567 (97.00%), Postives = 552/567 (97.35%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQGSGGTSAAGDSL HHQARVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSAQGSGGTSAAGDSLSHHQARVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLS TQIPQNLF+PFQPRE
Sbjct: 421 PGYAVVPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSCTQIPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHP GETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPPGETERTVG 556

BLAST of CmoCh04G004090 vs. NCBI nr
Match: KAG6600225.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.0e-297
Identity = 549/565 (97.17%), Postives = 551/565 (97.52%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQGSGGTSAAGDSL HHQARVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSAQGSGGTSAAGDSLSHHQARVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLF+PFQPRE
Sbjct: 421 PGYAVVPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERT 566
           SIASSSQSSSSSVSDSHP GETERT
Sbjct: 541 SIASSSQSSSSSVSDSHPPGETERT 554

BLAST of CmoCh04G004090 vs. NCBI nr
Match: XP_023006862.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 [Cucurbita maxima] >XP_023006934.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.7e-294
Identity = 543/567 (95.77%), Postives = 548/567 (96.65%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSS QGSGGTSAAGDSL HHQ+RVQAAAAAASIYEKSYVYPFGNTLAW           S
Sbjct: 361 SSSEQGSGGTSAAGDSLSHHQSRVQAAAAAASIYEKSYVYPFGNTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQVQRPLLDSNNVEPIGEQA NEQAQYFPF GGPANLS+TQIPQNLF+PFQPRE
Sbjct: 421 PGYAVVPQVQRPLLDSNNVEPIGEQAANEQAQYFPFAGGPANLSYTQIPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGL S QNISGSQLE+QERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK
Sbjct: 481 GTVNNTDGLSSGQNISGSQLEAQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHP GETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPPGETERTVG 556

BLAST of CmoCh04G004090 vs. NCBI nr
Match: XP_023531768.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo] >XP_023531774.1 ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018.5 bits (2632), Expect = 2.3e-293
Identity = 542/567 (95.59%), Postives = 546/567 (96.30%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL
Sbjct: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
           TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA
Sbjct: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM
Sbjct: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG
Sbjct: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT
Sbjct: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGLQSDANLQGTGTT 360
           VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQG QSDAN QGTGTT
Sbjct: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGGHQGSQSDANQQGTGTT 360

Query: 361 SSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFLLQNMWSLS 420
           SSSAQGSGGT A GDSL HHQARVQAAAAAASIYEKSYVYPF NTLAW           S
Sbjct: 361 SSSAQGSGGTGAVGDSLSHHQARVQAAAAAASIYEKSYVYPFANTLAW-----------S 420

Query: 421 PGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQPRE 480
           PGYAV+PQVQRPLLDSNNVEPIGEQA NEQAQYFPFT GPANLSFTQIPQNLF+PFQPRE
Sbjct: 421 PGYAVVPQVQRPLLDSNNVEPIGEQASNEQAQYFPFTSGPANLSFTQIPQNLFVPFQPRE 480

Query: 481 GTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKKGK 540
           GTVNNTDGLGSSQN SGSQLE+QERFLQQQIEILQDQLQLLRKLKVE+SSIAGEADKKGK
Sbjct: 481 GTVNNTDGLGSSQNTSGSQLEAQERFLQQQIEILQDQLQLLRKLKVEDSSIAGEADKKGK 540

Query: 541 SIASSSQSSSSSVSDSHPHGETERTVG 568
           SIASSSQSSSSSVSDSHP GETERTVG
Sbjct: 541 SIASSSQSSSSSVSDSHPPGETERTVG 556

BLAST of CmoCh04G004090 vs. TAIR 10
Match: AT3G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 597.8 bits (1540), Expect = 9.0e-171
Identity = 343/555 (61.80%), Postives = 403/555 (72.61%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++L+TYAG+SF+AT+ VIYHAF+SRGQFYPA VYLSTSKI+LVLLLNM LVLM  LW L
Sbjct: 1   MIRLRTYAGLSFMATLAVIYHAFSSRGQFYPATVYLSTSKISLVLLLNMCLVLMLSLWHL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFS  FL +V  LLLIKALHWLA
Sbjct: 61  VKFVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSSGFLPLVVTLLLIKALHWLA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV  LSH RIVSF+GFL ++DSLF+Y+SI  LIQ+R+ASVSLFFSFEYM
Sbjct: 121 QKRVEYIETTPSVSKLSHFRIVSFMGFLLLVDSLFMYSSIRHLIQSRQASVSLFFSFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT V+ FVKY+FYV+D+LM+GQWEKKPVYTFYLELIRDLLHLSMY+CFF VIFM YG
Sbjct: 181 ILATTTVAIFVKYVFYVTDMLMDGQWEKKPVYTFYLELIRDLLHLSMYICFFFVIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           VPLHL+RELYE FRNF+IR++DY+RYRKITSNMNDRFPDATPEEL +SDATCIICREEMT
Sbjct: 241 VPLHLLRELYETFRNFQIRVSDYLRYRKITSNMNDRFPDATPEELTASDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPEGSSTAGG-----HQGLQSDANLQ 360
            AKKL+CGHLFHVHCLRSWLERQ TCPTCRALVVPPE +++A       HQG Q      
Sbjct: 301 NAKKLICGHLFHVHCLRSWLERQQTCPTCRALVVPPENATSAAPGQRELHQGSQQ----- 360

Query: 361 GTGTTSSSAQGSGGTSAAG---DSLGHHQARVQAAAAAASIYEKSYVYPFGNTLAWYSFL 420
             GT+SS  QGS  +S+AG   +SL  H AR+QAAA+AAS+Y KS VYP  NT+AW S +
Sbjct: 361 --GTSSSGNQGSEISSSAGVSNNSLSRHHARLQAAASAASVYGKSMVYPSANTVAWSSGV 420

Query: 421 LQNMWSLSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNL 480
                                       P  EQ   E  Q  P    P   S        
Sbjct: 421 ----------------------------PGTEQVSTEPDQTLPQHNLPVENSH------- 480

Query: 481 FIPFQPREGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIA 540
                             +  N+S ++LE   + L+  +EIL+++L  L   K E    A
Sbjct: 481 ------------------AYANMSETKLEEMRKSLETHLEILRNRLHFLETRKPES---A 492

Query: 541 GEADKKGKSIASSSQ 548
           GE + KGKS+A +++
Sbjct: 541 GEPENKGKSVADAAE 492

BLAST of CmoCh04G004090 vs. TAIR 10
Match: AT1G65040.2 (RING/U-box superfamily protein )

HSP 1 Score: 588.6 bits (1516), Expect = 5.5e-168
Identity = 332/548 (60.58%), Postives = 395/548 (72.08%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINLV+LLNMGLVLM  LW L
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINLVVLLNMGLVLMLSLWNL 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
            K +FLGSLREAEVERLNEQ+W+ELMEILFAITIFRQDFSV F+++V  LLLIK LHW+A
Sbjct: 61  VKIVFLGSLREAEVERLNEQAWRELMEILFAITIFRQDFSVGFISLVVTLLLIKGLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI  LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANLQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA G++G   ++  QGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYP-FGNTLAWYSFLLQNMWS 420
           +SS  QGS  ++AA +++  H+AR QAAA+AASIY +S VYP   NTL W          
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVW---------- 420

Query: 421 LSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQP 480
            S G +++PQ                                                  
Sbjct: 421 -SQGNSLLPQ-------------------------------------------------- 460

Query: 481 REGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKK 540
                              +++E+Q RFL+ QIE+L +QL+LL K            D K
Sbjct: 481 -------------------TEVEAQRRFLESQIEVLTNQLRLLEK--------PTTVDTK 460

Query: 541 GKSIASSS 547
           GKS+A ++
Sbjct: 541 GKSVADTA 460

BLAST of CmoCh04G004090 vs. TAIR 10
Match: AT1G65040.3 (RING/U-box superfamily protein )

HSP 1 Score: 456.4 bits (1173), Expect = 3.2e-128
Identity = 276/548 (50.36%), Postives = 332/548 (60.58%), Query Frame = 0

Query: 1   MMKLQTYAGISFIATIVVIYHAFNSRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQL 60
           M++L+ YAG+S +AT+VVIYHAF+SRGQFYPA VYLSTSKINL                 
Sbjct: 1   MIQLKVYAGLSTLATLVVIYHAFSSRGQFYPATVYLSTSKINL----------------- 60

Query: 61  TKKIFLGSLREAEVERLNEQSWKELMEILFAITIFRQDFSVAFLAMVTALLLIKALHWLA 120
                                                                  LHW+A
Sbjct: 61  ------------------------------------------------------GLHWMA 120

Query: 121 QKRVEYMETTPSVPLLSHIRIVSFLGFLFVLDSLFLYNSIGSLIQTRKASVSLFFSFEYM 180
           QKRVEY+ETTPSV LLSH+RIVSF+ FL +LD L  Y+SI  LIQ+RKAS+S+FF+FEYM
Sbjct: 121 QKRVEYIETTPSVTLLSHVRIVSFMVFLLILDCLLTYSSIQQLIQSRKASMSVFFTFEYM 180

Query: 181 ILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTYG 240
           ILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM YG
Sbjct: 181 ILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNYG 240

Query: 241 VPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEMT 300
           +PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEMT
Sbjct: 241 LPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEMT 300

Query: 301 VAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANLQGTGT 360
            AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA G++G   ++  QGTGT
Sbjct: 301 SAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTGT 360

Query: 361 TSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYP-FGNTLAWYSFLLQNMWS 420
           +SS  QGS  ++AA +++  H+AR QAAA+AASIY +S VYP   NTL W          
Sbjct: 361 SSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVW---------- 389

Query: 421 LSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQP 480
            S G +++PQ                                                  
Sbjct: 421 -SQGNSLLPQ-------------------------------------------------- 389

Query: 481 REGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADKK 540
                              +++E+Q RFL+ QIE+L +QL+LL K            D K
Sbjct: 481 -------------------TEVEAQRRFLESQIEVLTNQLRLLEK--------PTTVDTK 389

Query: 541 GKSIASSS 547
           GKS+A ++
Sbjct: 541 GKSVADTA 389

BLAST of CmoCh04G004090 vs. TAIR 10
Match: AT1G65040.1 (RING/U-box superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 3.2e-91
Identity = 197/369 (53.39%), Postives = 236/369 (63.96%), Query Frame = 0

Query: 180 MILATTAVSTFVKYIFYVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMTY 239
           MILATT VS  VKY FYV+D+L EGQWE KPVYTFYLEL+RDLLHLSMYLCFFL+IFM Y
Sbjct: 1   MILATTTVSIIVKYAFYVTDMLKEGQWEGKPVYTFYLELVRDLLHLSMYLCFFLMIFMNY 60

Query: 240 GVPLHLIRELYEIFRNFRIRIADYIRYRKITSNMNDRFPDATPEELNSSDATCIICREEM 299
           G+PLHLIRELYE FRNF+IR+ DY+RYRKITSNMNDRFPDATPEEL+S+DATCIICREEM
Sbjct: 61  GLPLHLIRELYETFRNFKIRVTDYLRYRKITSNMNDRFPDATPEELSSNDATCIICREEM 120

Query: 300 TVAKKLVCGHLFHVHCLRSWLERQHTCPTCRALVVPPE-GSSTAGGHQGLQSDANLQGTG 359
           T AKKLVCGHLFHVHCLRSWLERQ+TCPTCRALVVP E  +STA G++G   ++  QGTG
Sbjct: 121 TSAKKLVCGHLFHVHCLRSWLERQNTCPTCRALVVPAENATSTASGNRGPHQESLQQGTG 180

Query: 360 TTSSSAQGSGGTSAAGDSLGHHQARVQAAAAAASIYEKSYVYP-FGNTLAWYSFLLQNMW 419
           T+SS  QGS  ++AA +++  H+AR QAAA+AASIY +S VYP   NTL W         
Sbjct: 181 TSSSDGQGSSVSAAASENMSRHEARFQAAASAASIYGRSIVYPSSANTLVW--------- 240

Query: 420 SLSPGYAVIPQVQRPLLDSNNVEPIGEQAPNEQAQYFPFTGGPANLSFTQIPQNLFIPFQ 479
             S G +++PQ                                                 
Sbjct: 241 --SQGNSLLPQ------------------------------------------------- 281

Query: 480 PREGTVNNTDGLGSSQNISGSQLESQERFLQQQIEILQDQLQLLRKLKVEESSIAGEADK 539
                               +++E+Q RFL+ QIE+L +QL+LL K            D 
Sbjct: 301 --------------------TEVEAQRRFLESQIEVLTNQLRLLEK--------PTTVDT 281

Query: 540 KGKSIASSS 547
           KGKS+A ++
Sbjct: 361 KGKSVADTA 281

BLAST of CmoCh04G004090 vs. TAIR 10
Match: AT4G25230.1 (RPM1 interacting protein 2 )

HSP 1 Score: 97.4 bits (241), Expect = 3.8e-20
Identity = 98/343 (28.57%), Postives = 154/343 (44.90%), Query Frame = 0

Query: 25  SRGQFYPALVYLSTSKINLVLLLNMGLVLMCILWQLTKKIFLGSLREAEVERLNEQSWKE 84
           S G    AL  L  S   + LL N  L +  +L    K +F G L + E ++L E+    
Sbjct: 42  SLGDSEHALELLLGSYFTIALLTNFVLNVYILLVLSLKTLFFGDLYDVETKKLVERLANY 101

Query: 85  L------MEILFAITIFRQDFSVAFLAMVTALLLIKALHWLAQKRVEYMETTPSVPLLSH 144
           +      + ++   TIF+    V +   +T L  +K    LA+ R+E +  +PS    ++
Sbjct: 102 IIYKGTFLPLVIPPTIFQ---GVLWTVWLTVLCTLKMFQALARDRLERLNASPSSTPWTY 161

Query: 145 IRIVSFLGFLFVLD------SLFLYNSIGSLIQTRKASVSLFFSFE----YMILATTAVS 204
            R+ S L  +  +D      SL  YN+IGS +           +FE     +I     + 
Sbjct: 162 FRVYSVLFLVLSVDMFWIKLSLMTYNTIGSAVYLLLLFEPCSIAFETLQALLIHGFQLLD 221

Query: 205 TFVKYIF----------YVSDVLMEGQWEKKPVYTFYLELIRDLLHLSMYLCFFLVIFMT 264
            ++ ++           ++  +      E K +    L    D+  L M L  +L I+  
Sbjct: 222 MWINHLAVKNSDCQRSKFIDSMTAGSLLEWKGLLNRNLGFFLDMATLVMALGHYLHIWWL 281

Query: 265 YGVPLHLIRELYEIFRNFRI-------RIADYIRYRKITSNMNDRFPDATPEELNSSDAT 324
           +G+  HL+  +  +F N R        RI  YI+ R     ++   PDAT EEL + D  
Sbjct: 282 HGIAFHLVDAV--LFLNIRALLSAILKRIKGYIKLRIALGALHAALPDATSEELRAYDDE 341

Query: 325 CIICREEMTVAKKLVCGHLFHVHCLRSWLER----QHTCPTCR 331
           C ICRE M  AK+L C HLFH+ CLRSWL++     ++CPTCR
Sbjct: 342 CAICREPMAKAKRLHCNHLFHLGCLRSWLDQGLNEVYSCPTCR 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LW771.3e-16961.80ERAD-associated E3 ubiquitin-protein ligase HRD1A OS=Arabidopsis thaliana OX=370... [more]
Q6NPT77.7e-16760.58ERAD-associated E3 ubiquitin-protein ligase HRD1B OS=Arabidopsis thaliana OX=370... [more]
Q5Z8802.6e-15959.51ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Oryza sativa subsp. japonica... [more]
Q803I82.5e-6943.20E3 ubiquitin-protein ligase synoviolin OS=Danio rerio OX=7955 GN=syvn1 PE=2 SV=2[more]
Q86TM61.3e-6843.20E3 ubiquitin-protein ligase synoviolin OS=Homo sapiens OX=9606 GN=SYVN1 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1FNJ31.1e-30198.06ERAD-associated E3 ubiquitin-protein ligase HRD1B-like OS=Cucurbita moschata OX=... [more]
A0A6J1KWZ51.3e-29495.77ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Cucurbita m... [more]
A0A6J1CJF52.3e-27088.89ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X1 OS=Momordica c... [more]
A0A6J1CI556.1e-26888.54ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 OS=Momordica c... [more]
A0A0A0LJG43.5e-26386.80RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G013330 P... [more]
Match NameE-valueIdentityDescription
XP_022942315.12.3e-30198.06ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita moschata] >XP_... [more]
KAG7030884.11.2e-29797.00ERAD-associated E3 ubiquitin-protein ligase HRD1A [Cucurbita argyrosperma subsp.... [more]
KAG6600225.12.0e-29797.17ERAD-associated E3 ubiquitin-protein ligase HRD1A, partial [Cucurbita argyrosper... [more]
XP_023006862.12.7e-29495.77ERAD-associated E3 ubiquitin-protein ligase HRD1B-like isoform X2 [Cucurbita max... [more]
XP_023531768.12.3e-29395.59ERAD-associated E3 ubiquitin-protein ligase HRD1B-like [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
AT3G16090.19.0e-17161.80RING/U-box superfamily protein [more]
AT1G65040.25.5e-16860.58RING/U-box superfamily protein [more]
AT1G65040.33.2e-12850.36RING/U-box superfamily protein [more]
AT1G65040.13.2e-9153.39RING/U-box superfamily protein [more]
AT4G25230.13.8e-2028.57RPM1 interacting protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 497..524
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 540..556
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 340..377
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 531..567
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 342..374
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 1..439
NoneNo IPR availablePANTHERPTHR22765:SF230ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE HRD1B-LIKEcoord: 1..439
NoneNo IPR availableCDDcd16479RING-H2_synoviolincoord: 289..330
e-value: 8.36427E-28
score: 103.532
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 272..336
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 292..329
e-value: 2.0E-5
score: 34.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 291..330
e-value: 1.2E-10
score: 41.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 292..330
score: 12.415513
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 264..336
e-value: 5.5E-23
score: 82.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G004090.1CmoCh04G004090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity