CmoCh04G004010 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G004010
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPhospholipid scramblase
LocationCmo_Chr04: 1977049 .. 1983249 (+)
RNA-Seq ExpressionCmoCh04G004010
SyntenyCmoCh04G004010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGATTCTGTTCATCGCCTGCAAATTCTAGAACCTCTAAAGCATTCTTATGCACAGAGCAAATTTTGATCTCTCTTTTTGCAATATTGTTGAAGTTTGATACTGCCGGAGGATTCTGTGGATGATATCGTAGATGAACTGGACTAGGAGTTCGCGTTTTTGGTCCAATACTGCTCGCAAATGCATCGGAGAAGATCGGTGCGGTAGGAGTTCGCTTTGGCCAGTGCAGAGAATTGCGCCTAGCTATCGTCATTTCGGAGGGAGGATTGTGAATGATGACTATCCACTTTTAGATAGGAGCTTCCTGGCGCAGCTGTGGGATGTAGATAGGAATTTGGGAAGCAGTAGAGAGAAGCGGAAGCGGTTATTGACTCGAAATGGAAATCAAAATCGAATAGCTCCCTATAATCCCAGGAATCTGGTATATGAACGTCCGTTTAGGTTATCGACTAAGGATGCGACTTTTGTAGAAGGGAAACAGTGGAAGCAACCGCCTCCAAGTCAATCTGTATCTGGTTTTCTAGCGCCGCATACTCCCGAGGAGGTAAGCTTAGATTTTAAGTTATATGCTTGCGCTTTGATTCTATGAAGTGGCCTGGTATCTGCAAGGACATCTCCTGCTATTTTCCGATCATAATAATGAAAAATAAATTTATATTCAACAGCAATATCAGTTCCTCCGTTCCTTGTCATTTCCAGTTGCAGTTTCCCTGAATAGTCTTGATTACCAGAATTAACTATGCTTTTTATCCTTCCGAAGGATTTCATTTGTTGAGAATTCATAATTGTTTCATCACGATCACGAACTGTTAATTAATTCATCATACGAATGATATAGTTTGTTTGTTGTCGTTGCTAAATAGTTCTGATCCAGTGTGCGTCGTTCCCACTGGAATGTAAAGCTATGTATTTGAATTTTAGTCAATTAGATCAGAATCTTTCACTCTTCTGTGTACTCTCTTCTCTCTGTGCTAAGAACGATTCTTCTTTCCGTATTGAGGTTCCACTTATGGGAGTGGATTAGTTCAGATTTATCGTCAAAGTGTGAGAAATTTTGTCCACCATGTATGTTTTCCCTAATAAGATACCATTATACCTCAAGTTTAGTTCATGTCAAATCCTAATGAGCAGAATATGGTTTGTGAGATCCCACATGGGTTGTAGAGCGGAATGAAACATTTCTTACAATGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAGAACCTTGAAGGGAAGTTCGGAAGGGAAAGCCCAAAGAGGACAATATCTACTAGCGTTGGGCTTGGGTTGTTACAAATGATATCAGAGCCAGACACCGAATGGTGTGCCAGCAAGGATGCTGGGCTCCCAAGGGGGTGAATTATGAGATCCCACATTGGTTGGAGAGGGGAATGAAACATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTGGACACGTTTTAAAACATTGAGGGAAAGTTCGAAAGAGAAAGTCCAAATAGAACAATATCTGCTAGCAGTGGGCTTGGGCTATTACATGGTTAGTCTTGAGTATTCGGGCACGACAATCGCTTATGCTTACCTCTTAGGTGAGTGTGATTACTAATTTGGGAATATGTTCTTTATCTTTTTGTGCATTTCATAGTAGGATGCTTGCTTCTTCTCACTTAATTTTTATTTCAAATTTATTGCAATTATAATTTTTTTCCTCTATCCTCTAGCATCACTATAACATGATTGTTTCCTTCCCCTCTCCATTAATTAGGTCCAAGTTGCACCTCTTCTTGCTAGATCTAATTTGCTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTCTTGGATTTGAGCAGGTAACTCTTTTCCATCACTTGTTTTCTCTTGGTTAGTTATTCACGTGCAGATTTCTGTCACACTTTTTATATCGTCCACTTATTTAACATAACTTCGGAAATTTTGGAGGATTAGAATGATGAAGTTAAGGTCCTTATCGATCAAATCCTAGCATCATCATAAGAGTGCAAAGAAATCATGTGTTATTTGATAGATGCTTTTTATTGAATTGCTCTTCCTGCTTTAACTTTCCTTTTTCTTTAGCAAGAATCATGTTGATGATTTTATGAAGTTGGCGATTTTACCACTTCTCTCATTTACTGTTGTCTTAGTAATATTTCTTTCTCGTGATTTTATCTGTATTTATTGGCCCCCTCTTCAAAGCATCTATATATGGACTCCAGATGATTATAATTTGTATTTATTTATACATTCTATCTACATGTTTTGAAAACTCCTGAACTCCGGATGATTATAATTTATATTTATTTATACATTCTATCTACATGTTTGGAAAACTCCTGGACCAATATTATGTGTCGTCCTTGACTGCTCCTTGGGATGTTTGTAGAAAGTTACCTTTAATAAGCAGTCATCTACACTTGAATAGCTTTACAGTTGCTAAGTTCGTCTATCATCAAATTCTTGCTTGCATTCCTGTTTCTTTACTTATAGATACTTTGGGTTGTGGTTATGCTATATCACCACTTTAATTTGAGAAATGCAGGAAAATCGTTATGCTATAGTAGACGTGTGCTATCCCCAGTCAGTAAGCATCCATTTTCATCTTTTAGTTTTCTTTTTTCCAGTTTTTACGCTCATCATATTCTTCTCTAACCTTATATACTGCAATGTTGTTTAACAGCCAGTTGGGTTTATTCGGGAGCAAAGTAACATCGTTGCTAGACAGGTATAATTTAGAATCTTTTGCGTTACCATGGTCTACTGTAAAACTATAATAACTGTGACTGTTCTGATCTGTAAATATGAAACTGAGCATATAGTGCTCGGGTAACTTCCCTGGTTCCATGCTGGCTTATAATTTCTCTTTTTCTTCCCCTCGTAAGAAAAATGAACAATTCATCTTATTAAAACTTCAAATGCTAATGCTTGATACATTTTGTTACAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACTGATGCAATGGGTAATGAACTTTTCAGGGTAAGCATCTTAATGTTTTTCTTCTCTATTTCAAGAATCAACTAAATTTGACTTTTAGATAGTTTTGTTAAAAAATGAAATATTATTGTGTTCTTATAGGTTCGACGGCCCTTTTGGTGGCTAACCAGTTCTATATATGCTGAAATTGATGGTAAGGTATGGTAGTTCAGTTGTCACATTAAGTGCATACAGTCGGAAATTCTTTTAGCTTGTGGCACTCTGGACATGTTTTCACATTAGAAAAACAACTCAGGAAATTGGTGTGGTTCATAGGCGATGGCATCTCTGGAGGAGGGTGTATGATCTGTACCTTGGGTAAGTTTAGCACAAACTATCTATCATCAATTCATAATATTCCATCAAACTGATTCTGATTGAAAGGGCATGCATGCATGCGTTTGTTGAGCAATTATTCAAGGAGGTTGGTATAATGTGGTTGACTGGTTGGAGATTTTATATTGTGGGTATAGAAGAGAGAAACTAAAAATTAAGATTTTACTGTGCATCCAAAGGTTAGAAGGGAGCCATCACAATTTGGCAGTCATATCATTTTGTTTTTGTGTATGATTTTGTTATTTCCTCTTCATTATTTCTTCCCTATGGTAATTTAAAGTACAACACGTTTAATCAAATTATCCTTAAAAAGTAACTATTAGTAAATTTAACATAACACAAGAATTATTACTTTGAGTATGTTTCCTTAAGATCTTATAATTGAAATAATACATCCATGACAACATCATTGTGTCATCCTTGTATGTATTAATTCCTTTTGTCTATCATGTTTGTGATTACACATTTGCATTATAGTATACAAAGTTGCGATGGGATTAACAGCCAATTTTATTCAAACAATTTTAATTATGACTTGACAATTTAAATTGTAACTATTAGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGCTTTTGGAATTGGACCTTCACATTAAAGGACATTGATGGTAATGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTCGGCTTCGAGGTTTTTTCTTTTCTCTTCCTGCAATAGCTTTATAATAAACCTATATTTGATACCTTTTAGCAACTTTCCGTTTAAAGAAATGTGCATTGTGGAAATAAAGAGATCCAATAATCTTCTTGCTCTCAGCATCCCTGTAGCAGTGCTTCCAAATCTGCCACGAGCCTAAGCCTCTTGCCTTTCTTATTGTAATAGAATAAGCTTAAATTGTATGTGCTTATTAAAAAAGAAAGGAAAAAAAATCATAGAGAATTTTTTGATAAAGAACACATATTTTTATATCAGAAACCAGCTTTTCATTTAGACAAATTGAAAGAATCAATGAAAACAAAAATGGAAAATAGAAAAAACAGCTAAACAAAAGGAACCTCCAGCAGCTAGACAAGAAATACAAAAAACAGGCTCCAATCCAGAAGGATAAGGCCTAAGAAAATTACAACAAAAACCCCCATAGTAAAGGATGATCAAAGAGACACATTGACTTAGGAAGGGTTCATGCCTCTTCACACGACCTCTTAAACCCAAAAGATTCTCCAATTTCTCTTTATTCAGATCCCACACACGAGAAAGTACAACGCCAAAGGACATTATCACGAAAAGGGTATGAAAGGGCATCTCCTCCATCATTGGAGGACAAGTCTCAGCTTTGAACCCACCTCTCACCGAAGCTCCCGAAAAAGGAAAAAAATGCCCAAATCAAATTAACAAAAAGACAGCTACAAGGAATGGATCCAAGTTCTCCGCCACTCCCCTACAAAAGATGCACCACTACAGCCTTGCACAATAAGAATAAAACTCAGAAGTCGATCCATTAGGAATTGAAATGCTCAAGAATATGAATCAAGACTTGTACGGATCTCCGAAATCAGGAATTTTTTGCCCTCGATATAATCAAATATTTGACCTTTGATTGCCGCTGGATTATAATGAAGTTTTTTTTTAGATATAATCAAATACCTACGGATCTGCCAGATTGCTTGCCTATTTAATAGTTTAACTTGCCGGGATATTGATCTTTTGTGGAATCTGGTTATACAACCCATTTCCTTCTTCCATAAGTGATTAAACTTCTTCCTGAGATATCTATTTATAGTCGATGAAACAAATTCCACGGCAAATATCTTTATCAATTGCTGTTCTCTGAAACTATTTTGCAGATGTTTACTGATGCTGGTCAGTATGTTATTCGTTTTGGTGCTTCAGATTCGGCTTCAAGGACTGGCCCCGCTAAAGGAGTAAAATTATTAGATTCATGTTTAACTTGATTTTAGTTTTCATACAATGTACTTTTTCAATATCGAGTGCTGTTGATGTTGACAGGTTCAAGAGCTGGAAGTCGCCCGACACCTTACTCTTTCAGAGAGAGCTGTAGCCGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGGGGCTGGTAAGTGGCAAACACTTCCTAAAACTTGCACTAAAGGATAATAACTATACTCTGTGTTCTTTCATTTTCCTCTGTGACAGTTCGTTTCTTTAAATACATATATAACCCAAATCCATTGTGACACTTAAGAGAACCCCATTTGAGATCAAATTCTCTGTGAACGGAGATTGACAGTGATGATTAGTTTATTTGCTAACAATCTCTAGAGAAGGAAGCCGTTGCAATGTGATTGGGAGTTAAAAAAAAAAAAAATTGATGAAACGCATTTCAAATTTGTATCTAGCGATTACCGATCATGAGAGGACTTAAAATTTTGAATCTATATTATTGAATCAAACACTGATTATTGAGTTGCTTATCATTTATCAGGGGACTTCCTTTCATTGCTGTTGGAGAGTAGAATTGGATTGCTGGACAGGCTTCTAGATCTCTGAGGTTTCGTCATTTTAATTCAATGAGTTTTTGATACCATATGTTGTATTTTTCATATTGATAAAGTTCTTTCGATCTTGGCGAAATCCCAAGTGTTATTAGCTTTGGGAAAAGAAAAAAAAAAACATATCACAAAAGAAAAATTAGAAAACTAATGGAACAGAATAAATTTTAGGCTCCATATTCTTCATCTTATTGCTGATTTCACGACTCAAGTGATGTTTATACTTGAAAATTGAGGTTTTGGATGTTTCTTTCGCCTCCTTTTGATTTCTTGGATGAGATCTCTTTTTTATGGGAATTTAATCTTGTTGACAAC

mRNA sequence

CACGATTCTGTTCATCGCCTGCAAATTCTAGAACCTCTAAAGCATTCTTATGCACAGAGCAAATTTTGATCTCTCTTTTTGCAATATTGTTGAAGTTTGATACTGCCGGAGGATTCTGTGGATGATATCGTAGATGAACTGGACTAGGAGTTCGCGTTTTTGGTCCAATACTGCTCGCAAATGCATCGGAGAAGATCGGTGCGGTAGGAGTTCGCTTTGGCCAGTGCAGAGAATTGCGCCTAGCTATCGTCATTTCGGAGGGAGGATTGTGAATGATGACTATCCACTTTTAGATAGGAGCTTCCTGGCGCAGCTGTGGGATGTAGATAGGAATTTGGGAAGCAGTAGAGAGAAGCGGAAGCGGTTATTGACTCGAAATGGAAATCAAAATCGAATAGCTCCCTATAATCCCAGGAATCTGGTATATGAACGTCCGTTTAGGTTATCGACTAAGGATGCGACTTTTGTAGAAGGGAAACAGTGGAAGCAACCGCCTCCAAGTCAATCTGTATCTGGTTTTCTAGCGCCGCATACTCCCGAGGAGGTCCAAGTTGCACCTCTTCTTGCTAGATCTAATTTGCTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTCTTGGATTTGAGCAGGAAAATCGTTATGCTATAGTAGACGTGTGCTATCCCCAGTCACCAGTTGGGTTTATTCGGGAGCAAAGTAACATCGTTGCTAGACAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACTGATGCAATGGGTAATGAACTTTTCAGGGTTCGACGGCCCTTTTGGTGGCTAACCAGTTCTATATATGCTGAAATTGATGGTAAGGAAATTGGTGTGGTTCATAGGCGATGGCATCTCTGGAGGAGGGTGTATGATCTGTACCTTGGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGCTTTTGGAATTGGACCTTCACATTAAAGGACATTGATGGTAATGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTCGGCTTCGAGATGTTTACTGATGCTGGTCAGTATGTTATTCGTTTTGGTGCTTCAGATTCGGCTTCAAGGACTGGCCCCGCTAAAGGAGTTCAAGAGCTGGAAGTCGCCCGACACCTTACTCTTTCAGAGAGAGCTGTAGCCGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGGGGCTGGGGACTTCCTTTCATTGCTGTTGGAGAGTAGAATTGGATTGCTGGACAGGCTTCTAGATCTCTGAGGTTTCGTCATTTTAATTCAATGAGTTTTTGATACCATATGTTGTATTTTTCATATTGATAAAGTTCTTTCGATCTTGGCGAAATCCCAAGTGTTATTAGCTTTGGGAAAAGAAAAAAAAAAACATATCACAAAAGAAAAATTAGAAAACTAATGGAACAGAATAAATTTTAGGCTCCATATTCTTCATCTTATTGCTGATTTCACGACTCAAGTGATGTTTATACTTGAAAATTGAGGTTTTGGATGTTTCTTTCGCCTCCTTTTGATTTCTTGGATGAGATCTCTTTTTTATGGGAATTTAATCTTGTTGACAAC

Coding sequence (CDS)

ATGAACTGGACTAGGAGTTCGCGTTTTTGGTCCAATACTGCTCGCAAATGCATCGGAGAAGATCGGTGCGGTAGGAGTTCGCTTTGGCCAGTGCAGAGAATTGCGCCTAGCTATCGTCATTTCGGAGGGAGGATTGTGAATGATGACTATCCACTTTTAGATAGGAGCTTCCTGGCGCAGCTGTGGGATGTAGATAGGAATTTGGGAAGCAGTAGAGAGAAGCGGAAGCGGTTATTGACTCGAAATGGAAATCAAAATCGAATAGCTCCCTATAATCCCAGGAATCTGGTATATGAACGTCCGTTTAGGTTATCGACTAAGGATGCGACTTTTGTAGAAGGGAAACAGTGGAAGCAACCGCCTCCAAGTCAATCTGTATCTGGTTTTCTAGCGCCGCATACTCCCGAGGAGGTCCAAGTTGCACCTCTTCTTGCTAGATCTAATTTGCTCATTACCAGGGATATAGAGTGGGCAAATCTGGTTCTTGGATTTGAGCAGGAAAATCGTTATGCTATAGTAGACGTGTGCTATCCCCAGTCACCAGTTGGGTTTATTCGGGAGCAAAGTAACATCGTTGCTAGACAGTTGCTTCGTCTAAGGCGCCCTTTTGTGGCATATATTACTGATGCAATGGGTAATGAACTTTTCAGGGTTCGACGGCCCTTTTGGTGGCTAACCAGTTCTATATATGCTGAAATTGATGGTAAGGAAATTGGTGTGGTTCATAGGCGATGGCATCTCTGGAGGAGGGTGTATGATCTGTACCTTGGGAACAAGCAGTTCGCAGTTGTTGAAAATCCTGGCTTTTGGAATTGGACCTTCACATTAAAGGACATTGATGGTAATGTGCTTGCTGAGGTGGATCGTGACTGGAGAGGTTTCGGCTTCGAGATGTTTACTGATGCTGGTCAGTATGTTATTCGTTTTGGTGCTTCAGATTCGGCTTCAAGGACTGGCCCCGCTAAAGGAGTTCAAGAGCTGGAAGTCGCCCGACACCTTACTCTTTCAGAGAGAGCTGTAGCCGTTGCTCTTGCTATTTCACTTGATAATGACTATTTCTCAAGACATGGGGGCTGGGGACTTCCTTTCATTGCTGTTGGAGAGTAG

Protein sequence

MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQLWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFTDAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLPFIAVGE
Homology
BLAST of CmoCh04G004010 vs. ExPASy Swiss-Prot
Match: P47140 (Altered inheritance rate of mitochondria protein 25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=AIM25 PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.8e-37
Identity = 102/295 (34.58%), Postives = 147/295 (49.83%), Query Frame = 0

Query: 101 PFRLSTKDATFVEGKQWKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANL 160
           PFR   ++   +      +     S SG +  H P       +L    ++I R IE+ N+
Sbjct: 36  PFRRVIREKNQITKAPSVKAFKENSNSGIIKVHDP---IATTILNEPTVIIERQIEFMNV 95

Query: 161 VLGFEQENRYAIVDVCYPQSPVGFIREQS--NIVARQLLRLRRPFVAYITDAMGNELFRV 220
            LGFEQ NRYAI+DV   +      R+ S    + RQ  RL RPF+  + D  GN +  +
Sbjct: 96  FLGFEQANRYAIMDVNGNKIASMMERDFSITKAIMRQFYRLHRPFLVDVFDNWGNVIMTI 155

Query: 221 RRPFWWLTSSIYAEI----------------DGKE---IGVVHRRWHLWRRVYDLYLGN- 280
           +RPF ++ S I   I                DGKE   +G   + WHLWRR Y+L+  + 
Sbjct: 156 KRPFSFINSHIKTIIPPSAYVDNGSDSTHYHDGKEGTTVGETIQNWHLWRRRYELFQKDG 215

Query: 281 ------KQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFTDAGQYVIRFGAS 340
                  QF  ++ P F ++ F + D DG ++A VDR+W G G EMFTD G YV+RF + 
Sbjct: 216 VEGSTFDQFGKIDAP-FLSFDFPVTDADGKIMASVDRNWVGLGREMFTDTGVYVVRFDSQ 275

Query: 341 DSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLPFIAVG 368
                  P     E+  ++ LTL +RAV +A A+S+D DYFSRH      F++ G
Sbjct: 276 RCFDNIYPT----EMLSSQVLTLDQRAVLLANAVSIDFDYFSRHSRQTGGFLSFG 322

BLAST of CmoCh04G004010 vs. ExPASy Swiss-Prot
Match: Q9UT84 (Phospholipid scramblase family protein C343.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC343.06c PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.2e-34
Identity = 92/274 (33.58%), Postives = 147/274 (53.65%), Query Frame = 0

Query: 108 DATFVEGKQWKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQE 167
           DAT    K   Q P + S +G +  ++P     APLL++  L++ R +E  N+ LG+EQ 
Sbjct: 45  DAT---AKVVSQEPAAISSTGAIPLNSP----AAPLLSQDVLIVERQLEMMNVFLGYEQA 104

Query: 168 NRYAIVDVCYPQSPVGFIREQS-----NIVARQLLRLRRPFVAYITDAMGNELFRVRRPF 227
           NRY I++       +G+I EQ      + ++RQ     R F A + D+ G  + ++ RPF
Sbjct: 105 NRYVILN--QQGQHLGYIAEQGASSILSSLSRQFFHTHRAFKADVMDSNGQLVLQLNRPF 164

Query: 228 WWLTSSI------YAEIDGKEIGVVHRRWHLWRRVYDLYLGNK----QFAVVENPGFWNW 287
            W+ S +      Y++     +G V ++WHLWRR Y+L+L  +    QFA ++     +W
Sbjct: 165 SWINSRLQIHSIDYSKFSSTLVGEVLQKWHLWRRRYELFLAKRSMFEQFAKIDE-RVLSW 224

Query: 288 TFTLKDIDGNVLAEVDRDWRGFGFEMFTDAGQYVIRFGASDSASRT-------GPAKGVQ 347
            F L++    +L  V R++ G   E FTD G YV+RF ++ +A+ +         A G+ 
Sbjct: 225 EFLLRNEQDRILGSVSRNFMGLPREFFTDTGNYVLRFTSTSAANGSVNENQLLQAAHGIA 284

Query: 348 ELEVARHLTLSERAVAVALAISLDNDYFSR-HGG 359
               AR ++L ERAV +  A+++D DYFSR HGG
Sbjct: 285 NDVCARDMSLEERAVMLGSAVTIDFDYFSRIHGG 308

BLAST of CmoCh04G004010 vs. ExPASy Swiss-Prot
Match: Q9NRQ2 (Phospholipid scramblase 4 OS=Homo sapiens OX=9606 GN=PLSCR4 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 8.6e-10
Identity = 58/213 (27.23%), Postives = 87/213 (40.85%), Query Frame = 0

Query: 118 KQPPPSQSVSGFLAPHTPEEVQVAP----LLARSNLLITRDIEWANLVLGFEQENRYAIV 177
           K P P+QSV     P         P    L+   N+ + +  E   ++  FE  NRY I 
Sbjct: 87  KYPMPNQSVPITWMPGPTPMANCPPGLEYLVQLDNIHVLQHFEPLEMMTCFETNNRYDIK 146

Query: 178 DVCYPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPF-----WWLTSS 237
           +       V  + E ++   R   R  RPFV  +TD MG E+  ++RPF      +   S
Sbjct: 147 N--NSDQMVYIVTEDTDDFTRNAYRTLRPFVLRVTDCMGREIMTMQRPFRCTCCCFCCPS 206

Query: 238 IYAEID-----GKEIGVVHRRWHLWRRVYDLYLGNKQFAV-VENPGFW-----NWTFTLK 297
              E++     G  IG V   W+L R VY +    K+  + V  P        +  F +K
Sbjct: 207 ARQELEVQCPPGVTIGFVAEHWNLCRAVYSIQNEKKENVMRVRGPCSTYGCGSDSVFEVK 266

Query: 298 DIDG-NVLAEVDRDWRGFGFEMFTDAGQYVIRF 310
            +DG + +  + R W G       DA  + I F
Sbjct: 267 SLDGISNIGSIIRKWNGL-LSAMADADHFDIHF 296

BLAST of CmoCh04G004010 vs. ExPASy Swiss-Prot
Match: P58196 (Phospholipid scramblase 4 OS=Mus musculus OX=10090 GN=Plscr4 PE=2 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 6.2e-08
Identity = 54/209 (25.84%), Postives = 88/209 (42.11%), Query Frame = 0

Query: 119 QPPPSQSVSG-FLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCY 178
           QP P   ++G    P+ P  ++   L    N+ + + +E   L+  FE  NRY I +   
Sbjct: 89  QPAPIMWMAGPAPVPNCPPGLEY--LAQLDNIHVLQHVEPLELMTRFETNNRYDIKNNI- 148

Query: 179 PQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPF-----WWLTSSIYAE 238
               V  + E ++   R   R  RPFV  +TD +G E+  ++RPF      +       E
Sbjct: 149 -DQMVYIVTEDTDDFTRNAYRNLRPFVLRVTDCLGREIMTMQRPFRCTCCCFCCPCARQE 208

Query: 239 ID-----GKEIGVVHRRWHLWRRVYDLYLGNKQFAV-VENPGFW-----NWTFTLKDIDG 298
           ++     G  IG V   W+L R  Y +    K+  + V  P        +  F +  +DG
Sbjct: 209 LEVQCPPGVTIGFVAEHWNLCRASYSIQNEKKESMMRVRGPCATYGCGSDSVFEINSLDG 268

Query: 299 -NVLAEVDRDWRGFGFEMFTDAGQYVIRF 310
            + +  + R W GF      +A  + IRF
Sbjct: 269 VSNIGSIIRKWNGF-LSTMVNADHFEIRF 292

BLAST of CmoCh04G004010 vs. ExPASy Swiss-Prot
Match: Q9NRY7 (Phospholipid scramblase 2 OS=Homo sapiens OX=9606 GN=PLSCR2 PE=1 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 8.9e-07
Identity = 47/177 (26.55%), Postives = 73/177 (41.24%), Query Frame = 0

Query: 149 LLITRDIEWANLVLGFEQENRYAIVDVCYPQSPVGFIREQSNIVARQLLRLRRPFVAYIT 208
           +LI + IE   ++  FE  N Y I +  + Q  + F  E +N   R      RPF   IT
Sbjct: 96  ILIHQQIELLEVLFSFESSNMYEIKN-SFGQR-IYFAAEDTNFCIRNCCGRSRPFTLRIT 155

Query: 209 DAMGNELFRVRRPF---WWLTSSIYAEID-----GKEIGVVHRRWHLWRRVYDLYLGNKQ 268
           D +G E+  + RP             EI+     G  +G V + WH     + +    K+
Sbjct: 156 DNVGREVITLERPLRCNCCCCPCCLQEIEIQAPPGVPVGYVTQTWHPCLTKFTI-KNQKR 215

Query: 269 FAVVENPG-------FWNWTFTLKDIDGN-VLAEVDRDWRGFGFEMFTDAGQYVIRF 310
             V++  G            F +  +D   V+  + + W GF  E FTDA  + I+F
Sbjct: 216 EDVLKISGPCIVCSCIAGVDFEITSLDEQIVVGRISKHWSGFLREAFTDADNFGIQF 269

BLAST of CmoCh04G004010 vs. ExPASy TrEMBL
Match: A0A6J1FQC4 (Phospholipid scramblase OS=Cucurbita moschata OX=3662 GN=LOC111447826 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 5.3e-220
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ
Sbjct: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. ExPASy TrEMBL
Match: A0A6J1L1X4 (Phospholipid scramblase OS=Cucurbita maxima OX=3661 GN=LOC111498343 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 2.7e-216
Identity = 364/368 (98.91%), Postives = 364/368 (98.91%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNTARKCIGEDRCGRSSLW VQRIAPSYRHFGGRIVN DYPLLDRSFLAQ
Sbjct: 1   MNWTRSLRFWSNTARKCIGEDRCGRSSLWAVQRIAPSYRHFGGRIVNVDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYN RNLVYERPFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNHRNLVYERPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. ExPASy TrEMBL
Match: A0A6J1CIB1 (Phospholipid scramblase OS=Momordica charantia OX=3673 GN=LOC111011741 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.7e-187
Identity = 315/368 (85.60%), Postives = 333/368 (90.49%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RF SNT RKCIGED   RSS  P++ IAP+YRHFG R  NDDYPLLDR FLAQ
Sbjct: 1   MNWTRSLRFLSNTVRKCIGEDCRARSSRRPLRSIAPTYRHFGRRFENDDYPLLDRRFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LW+ DR L  SREKRKR+   +GN+ RI+ YN RNL++ERPF  ST DA F EGK WKQP
Sbjct: 61  LWEADRKLERSREKRKRISGGHGNRKRISHYNHRNLLFERPFGKSTSDAAFAEGKVWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSG L PH+PEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGVLEPHSPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNI+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAE+DGKEIGV
Sbjct: 181 PVGFIREQSNIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFA VENPGFW+WTFTLKDIDG VLAE+DRDWRGFGFE+FT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFATVENPGFWSWTFTLKDIDGEVLAEIDRDWRGFGFEIFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASD  SRTGPA+GVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDPVSRTGPARGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. ExPASy TrEMBL
Match: A0A5A7TN09 (Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004590 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.3e-186
Identity = 320/372 (86.02%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNT RKCI ED CGR+SLWPV RIAP YRHFG RI NDD PL+DRSFLA+
Sbjct: 53  MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 112

Query: 61  LWDVDRNLGSSREKRKR----LLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQ 120
           LW+ DR L  SREKRKR       RNGNQNRI  Y+       R F  ST DATF EGK 
Sbjct: 113 LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYD------RRAFGKSTLDATFAEGKP 172

Query: 121 WKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVC 180
           WKQPPPSQSVSGFL PH+PEEVQVAPLLARSNLLITRDIEWANLV GFEQENRYAIVDVC
Sbjct: 173 WKQPPPSQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVC 232

Query: 181 YPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGK 240
           YPQSPVGFIREQS+I+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAE+DGK
Sbjct: 233 YPQSPVGFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGK 292

Query: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGF 300
           EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDG VLAEVDRDWRGFGF
Sbjct: 293 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGF 352

Query: 301 EMFTDAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360
           E+FTDAGQYVIRFGASD  SRTGPA+GVQELEVAR LTLSERAVAVALAISLDNDYFSRH
Sbjct: 353 EIFTDAGQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRH 412

Query: 361 GGWGLPFIAVGE 369
           GGWGLPFIAVGE
Sbjct: 413 GGWGLPFIAVGE 418

BLAST of CmoCh04G004010 vs. ExPASy TrEMBL
Match: A0A5D3CZC9 (Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00160 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.3e-186
Identity = 320/372 (86.02%), Postives = 331/372 (88.98%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNT RKCI ED CGR+SLWPV RIAP YRHFG RI NDD PL+DRSFLA+
Sbjct: 1   MNWTRSLRFWSNTVRKCIVEDCCGRTSLWPVHRIAPGYRHFGRRIQNDDSPLIDRSFLAK 60

Query: 61  LWDVDRNLGSSREKRKR----LLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQ 120
           LW+ DR L  SREKRKR       RNGNQNRI  Y+       R F  ST DATF EGK 
Sbjct: 61  LWEADRKLEGSREKRKRHRIGNYDRNGNQNRIGGYD------RRAFGKSTLDATFAEGKP 120

Query: 121 WKQPPPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVC 180
           WKQPPPSQSVSGFL PH+PEEVQVAPLLARSNLLITRDIEWANLV GFEQENRYAIVDVC
Sbjct: 121 WKQPPPSQSVSGFLEPHSPEEVQVAPLLARSNLLITRDIEWANLVFGFEQENRYAIVDVC 180

Query: 181 YPQSPVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGK 240
           YPQSPVGFIREQS+I+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAE+DGK
Sbjct: 181 YPQSPVGFIREQSSIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEVDGK 240

Query: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGF 300
           EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDG VLAEVDRDWRGFGF
Sbjct: 241 EIGVVHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGKVLAEVDRDWRGFGF 300

Query: 301 EMFTDAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRH 360
           E+FTDAGQYVIRFGASD  SRTGPA+GVQELEVAR LTLSERAVAVALAISLDNDYFSRH
Sbjct: 301 EIFTDAGQYVIRFGASDPVSRTGPARGVQELEVARQLTLSERAVAVALAISLDNDYFSRH 360

Query: 361 GGWGLPFIAVGE 369
           GGWGLPFIAVGE
Sbjct: 361 GGWGLPFIAVGE 366

BLAST of CmoCh04G004010 vs. NCBI nr
Match: XP_022942941.1 (altered inheritance rate of mitochondria protein 25 [Cucurbita moschata])

HSP 1 Score: 773.1 bits (1995), Expect = 1.1e-219
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ
Sbjct: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. NCBI nr
Match: KAG6600218.1 (hypothetical protein SDJN03_05451, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030877.1 AIM25 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.3 bits (1980), Expect = 6.0e-218
Identity = 366/368 (99.46%), Postives = 366/368 (99.46%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ
Sbjct: 1   MNWTRSLRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYN RNLVYERPFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNHRNLVYERPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. NCBI nr
Match: XP_023005343.1 (altered inheritance rate of mitochondria protein 25 [Cucurbita maxima])

HSP 1 Score: 760.8 bits (1963), Expect = 5.6e-216
Identity = 364/368 (98.91%), Postives = 364/368 (98.91%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNTARKCIGEDRCGRSSLW VQRIAPSYRHFGGRIVN DYPLLDRSFLAQ
Sbjct: 1   MNWTRSLRFWSNTARKCIGEDRCGRSSLWAVQRIAPSYRHFGGRIVNVDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYN RNLVYERPFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNHRNLVYERPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. NCBI nr
Match: XP_023512311.1 (altered inheritance rate of mitochondria protein 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.1 bits (1951), Expect = 1.4e-214
Identity = 362/368 (98.37%), Postives = 362/368 (98.37%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RFWSNTARKCIGEDRCGRSSLWPVQRIA SYRHFGGRIVNDDYPLLDRSFLAQ
Sbjct: 1   MNWTRSLRFWSNTARKCIGEDRCGRSSLWPVQRIATSYRHFGGRIVNDDYPLLDRSFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LWDVDRNLGSSREKRKRLLTRNGNQNRIA YN RNLVYE PFRLSTKDATFVEGKQWKQP
Sbjct: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAHYNHRNLVYESPFRLSTKDATFVEGKQWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV
Sbjct: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASD ASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDLASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. NCBI nr
Match: XP_022141314.1 (altered inheritance rate of mitochondria protein 25 isoform X1 [Momordica charantia])

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-186
Identity = 315/368 (85.60%), Postives = 333/368 (90.49%), Query Frame = 0

Query: 1   MNWTRSSRFWSNTARKCIGEDRCGRSSLWPVQRIAPSYRHFGGRIVNDDYPLLDRSFLAQ 60
           MNWTRS RF SNT RKCIGED   RSS  P++ IAP+YRHFG R  NDDYPLLDR FLAQ
Sbjct: 1   MNWTRSLRFLSNTVRKCIGEDCRARSSRRPLRSIAPTYRHFGRRFENDDYPLLDRRFLAQ 60

Query: 61  LWDVDRNLGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQP 120
           LW+ DR L  SREKRKR+   +GN+ RI+ YN RNL++ERPF  ST DA F EGK WKQP
Sbjct: 61  LWEADRKLERSREKRKRISGGHGNRKRISHYNHRNLLFERPFGKSTSDAAFAEGKVWKQP 120

Query: 121 PPSQSVSGFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180
           PPSQSVSG L PH+PEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS
Sbjct: 121 PPSQSVSGVLEPHSPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQS 180

Query: 181 PVGFIREQSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGV 240
           PVGFIREQSNI+ARQLLRLRRPFVAYITDAMGNELFRVRRPFWW+TSSIYAE+DGKEIGV
Sbjct: 181 PVGFIREQSNIIARQLLRLRRPFVAYITDAMGNELFRVRRPFWWITSSIYAEVDGKEIGV 240

Query: 241 VHRRWHLWRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFT 300
           VHRRWHLWRRVYDLYLGNKQFA VENPGFW+WTFTLKDIDG VLAE+DRDWRGFGFE+FT
Sbjct: 241 VHRRWHLWRRVYDLYLGNKQFATVENPGFWSWTFTLKDIDGEVLAEIDRDWRGFGFEIFT 300

Query: 301 DAGQYVIRFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360
           DAGQYVIRFGASD  SRTGPA+GVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG
Sbjct: 301 DAGQYVIRFGASDPVSRTGPARGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWG 360

Query: 361 LPFIAVGE 369
           LPFIAVGE
Sbjct: 361 LPFIAVGE 368

BLAST of CmoCh04G004010 vs. TAIR 10
Match: AT2G04940.1 (scramblase-related )

HSP 1 Score: 464.9 bits (1195), Expect = 5.9e-131
Identity = 229/361 (63.43%), Postives = 270/361 (74.79%), Query Frame = 0

Query: 22  RCGRSSLWPVQRIAPSYRHF---GGRIVNDDY-----------PLLDRSFLAQLWDVDRN 81
           R G + LW   ++  S   F   GG++    +           P LDR FLAQLW  D+ 
Sbjct: 47  RAGNAVLWRNSQLCSSQGSFFGRGGQVSGSVFRCFCSGSDTSPPSLDRKFLAQLWVADKK 106

Query: 82  LGSSREKRKRLLTRNGNQNRIAPYNPRNLVYERPFRLSTKDATFVEGKQWKQPPPSQSVS 141
              + EKR +  +++ N      ++             T + T       +QPP SQS+S
Sbjct: 107 KLKAMEKRYKKASKHRNYTENDGFD---------VHFETVEPTV------EQPPVSQSMS 166

Query: 142 GFLAPHTPEEVQVAPLLARSNLLITRDIEWANLVLGFEQENRYAIVDVCYPQSPVGFIRE 201
           G L P T +E ++A LLARSNLL+TRDIEWANLVLGFEQENRY +VDVCYP++PVG IRE
Sbjct: 167 GLLKPKTSDEAKIATLLARSNLLVTRDIEWANLVLGFEQENRYIVVDVCYPEAPVGSIRE 226

Query: 202 QSNIVARQLLRLRRPFVAYITDAMGNELFRVRRPFWWLTSSIYAEIDGKEIGVVHRRWHL 261
           QSN++ARQLLR RRPFVA ITDA+GNELFRVRRPFWWLTSSIYAEIDG+EIGVVHRRWHL
Sbjct: 227 QSNLLARQLLRTRRPFVASITDALGNELFRVRRPFWWLTSSIYAEIDGEEIGVVHRRWHL 286

Query: 262 WRRVYDLYLGNKQFAVVENPGFWNWTFTLKDIDGNVLAEVDRDWRGFGFEMFTDAGQYVI 321
           WRR+YDLYLGN QFAVVENPGFWNWTFT+KD DG VLA++DRDWRGFGFE+FTDAGQYVI
Sbjct: 287 WRRIYDLYLGNNQFAVVENPGFWNWTFTVKDADGEVLAQIDRDWRGFGFEIFTDAGQYVI 346

Query: 322 RFGASDSASRTGPAKGVQELEVARHLTLSERAVAVALAISLDNDYFSRHGGWGLPFIAVG 369
           RFG +D+A++TGPA  V+ELEV R LTLSERAV + LAISLDNDYFSRHGGWG+PF+AVG
Sbjct: 347 RFGKADAAAKTGPATLVEELEVKRPLTLSERAVVLTLAISLDNDYFSRHGGWGIPFMAVG 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P471404.8e-3734.58Altered inheritance rate of mitochondria protein 25 OS=Saccharomyces cerevisiae ... [more]
Q9UT842.2e-3433.58Phospholipid scramblase family protein C343.06c OS=Schizosaccharomyces pombe (st... [more]
Q9NRQ28.6e-1027.23Phospholipid scramblase 4 OS=Homo sapiens OX=9606 GN=PLSCR4 PE=1 SV=2[more]
P581966.2e-0825.84Phospholipid scramblase 4 OS=Mus musculus OX=10090 GN=Plscr4 PE=2 SV=2[more]
Q9NRY78.9e-0726.55Phospholipid scramblase 2 OS=Homo sapiens OX=9606 GN=PLSCR2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FQC45.3e-220100.00Phospholipid scramblase OS=Cucurbita moschata OX=3662 GN=LOC111447826 PE=3 SV=1[more]
A0A6J1L1X42.7e-21698.91Phospholipid scramblase OS=Cucurbita maxima OX=3661 GN=LOC111498343 PE=3 SV=1[more]
A0A6J1CIB17.7e-18785.60Phospholipid scramblase OS=Momordica charantia OX=3673 GN=LOC111011741 PE=3 SV=1[more]
A0A5A7TN091.3e-18686.02Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A5D3CZC91.3e-18686.02Phospholipid scramblase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
XP_022942941.11.1e-219100.00altered inheritance rate of mitochondria protein 25 [Cucurbita moschata][more]
KAG6600218.16.0e-21899.46hypothetical protein SDJN03_05451, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023005343.15.6e-21698.91altered inheritance rate of mitochondria protein 25 [Cucurbita maxima][more]
XP_023512311.11.4e-21498.37altered inheritance rate of mitochondria protein 25 [Cucurbita pepo subsp. pepo][more]
XP_022141314.11.6e-18685.60altered inheritance rate of mitochondria protein 25 isoform X1 [Momordica charan... [more]
Match NameE-valueIdentityDescription
AT2G04940.15.9e-13163.43scramblase-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005552ScramblasePFAMPF03803Scramblasecoord: 127..355
e-value: 6.4E-66
score: 221.8
IPR005552ScramblasePANTHERPTHR23248PHOSPHOLIPID SCRAMBLASE-RELATEDcoord: 114..361
NoneNo IPR availablePANTHERPTHR23248:SF9PHOSPHOLIPID SCRAMBLASEcoord: 114..361
IPR025659Tubby-like, C-terminalSUPERFAMILY54518Tubby C-terminal domain-likecoord: 206..308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G004010.1CmoCh04G004010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017121 plasma membrane phospholipid scrambling
cellular_component GO:0005886 plasma membrane
molecular_function GO:0017128 phospholipid scramblase activity