CmoCh04G002480 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G002480
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCmo_Chr04: 1229697 .. 1232459 (-)
RNA-Seq ExpressionCmoCh04G002480
SyntenyCmoCh04G002480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGCATTGTTTTTGTTTAAATTCCCCTTCTTCTCTGCTCCAAATCTCATTCCTACACCCTCTGTTCTTCTGGGTTTTTCATAAATTTTCTCACCATCCAAACAGATTCTCAATTTTCTCTCAGATCCACTTTTCTGTTCTTAAACGCAATCCAGATGGAGAATGTGACATACGAAGAGGTGAGTTCATGTTTTTTCTTTTAATTTTTGGGATTTTCCCGAGAAAATCAACGGGAAAATTTTGAAAATTTGCAGGAATTTATAGTGAATTCACGTGGGTTGAAGCTCTTCACGTGCAGTTGGGTTCCCAAGAATGATGAACCAAAAGCTCTGGTTTTTCTCTGCCATGGCTACGCCATGGAATGTAGCATCACCATGGACAGTAAGTTTTCTTTTTATTATTTATTTATATATTTATTATCATTCCTTAAATTAGCCGTTATTTATTTTGAATTTTATATATTCCATAATTATTCCTTAAATTAGCCTATTTATTTTGAATAGGTAGTGCAAGGAGACTGGCAGAGGAAGGGTATGGAGTTTATGGGATTGATTACGAAGGACATGGGAAGTCCTCTGGGTTACAAGGCTATGTTTCAAGTTTCGATAAAGTAGTCGACGACTGTTCTTCGTTCTTCACTTCCATAACGGGTAACTAATTCGTTTTCTTATCATTATTGGACTCTTGAGAGGTCAGAAGTTCAAATTTCTAGAAAATTATGAGTTATTTTGTTTAAATTAGCGGATAAAATTAATTAAAATAATGAATTATGAGCAGAAAAGAATGAAAATAGGGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCGGTGGCTCTATTGGTACACAGAAAACAGCCTGATTATTGGGATGGAGCCATCTTAGTTGCTCCCATGTGTAAGGTCATTTTTTTACTCCTTTATTTATTTATTTTTATTTTTATTTTCAATTATGTTTTATAATTAAAATAAAACAGGATAATTAAGCAAATGGAGATATAATATTAATATTAATATTAATATTAATGTGTGATGTGCAAGCAAAGGTTTGTTAAATGATTGCATTAAAAAATAATTAATACATTATTATTTTAATTGACATCTCAACAAAAAAAAAATGTCAAATTCAAAATTTTTTAATTTTTAATTAAATTTAAAAAATAAGTGATTTAGATCTACAGATTGGACTTTTTCCATACGTTGGAATCTTGAAAAGCTAATCTCGATTATTGAGCTGATTTTTTTTTTTTTTTTTAATTTTGACCCATTTAATATTTATTTTAGAAATAATAAAAAAGAGGGACTAAAGGGTAATTTCATATTTAAATTATAAATAATTGGGAAATTTAGATGTATTTGATTTGATTTTATGTTAATTATTTAATGAATATGTGGTGTGGAATTTGATAGATTGCTGAGGACATGAGACCAAATCCTTTGACGATAAGTTTTCTGACAAAGCTTTGCAAAATAATTCCAACCTGGAAAATTATTCCAACTCAAGATATTATTGATATTGCCTTCAAAGTCCCTGAAATTAGAAAACAGGTATTTATTTTAATTTCCATTTTTGATTTTATTATTATAAATGCAAATAATGGAAAATAATGTTGTTATTTGCAGATTAGGGAAAATCGGTACTGTTATAAAGGTCGACCTCGATTGAAAACTGGCCATGAACTCTTGAGGATTAGCTCAGAACTTGAACATAAGCTCGATGAGGTATTTTTGTTTTGTTTTTATTTATTTTTGTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAATTATATGTTGGGTGTCCGGTTTAGGTTTCTGTACCATTCCTCCTTCTTCATGGTGAAGATGATCGAGTTACGGACAGATTGGTTAGCAAACAACTCTACGACGAAGCAGTGAGCGATGATAAGACGTTGAAGATGTACCCGGGAATGTGGCATGGTTTGTTGTACGGCGAGACACCGGAGAATATAGATATTGTATTCTCAGACATCATCGACTGGTTGGAGAAGAGGAATGTGAAGTCGGAACTTGAGAAGAAGTATGAAAATGATCATCTCGGTGGCTTGGAATCCAAGCAACAATAAATTCATTAATTGTTATTCTAAAGAGTCCTTTTCGATTTGTTGGGAAAGAAAGTTGTGATATCGTTTTTAATCATATTGTAAATATTTATGAGTAATTTCAAAATTTATAGGTGAACGTTAATAAAGTTGCGTTTGAATGTTATTTGATGGTTAA

mRNA sequence

TTTGCATTGTTTTTGTTTAAATTCCCCTTCTTCTCTGCTCCAAATCTCATTCCTACACCCTCTGTTCTTCTGGGTTTTTCATAAATTTTCTCACCATCCAAACAGATTCTCAATTTTCTCTCAGATCCACTTTTCTGTTCTTAAACGCAATCCAGATGGAGAATGTGACATACGAAGAGGAATTTATAGTGAATTCACGTGGGTTGAAGCTCTTCACGTGCAGTTGGGTTCCCAAGAATGATGAACCAAAAGCTCTGGTTTTTCTCTGCCATGGCTACGCCATGGAATGTAGCATCACCATGGACAGTAGTGCAAGGAGACTGGCAGAGGAAGGGTATGGAGTTTATGGGATTGATTACGAAGGACATGGGAAGTCCTCTGGGTTACAAGGCTATGTTTCAAGTTTCGATAAAGTAGTCGACGACTGTTCTTCGTTCTTCACTTCCATAACGGAAAAGAATGAAAATAGGGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCGGTGGCTCTATTGGTACACAGAAAACAGCCTGATTATTGGGATGGAGCCATCTTAGTTGCTCCCATGTGTAAGATTGCTGAGGACATGAGACCAAATCCTTTGACGATAAGTTTTCTGACAAAGCTTTGCAAAATAATTCCAACCTGGAAAATTATTCCAACTCAAGATATTATTGATATTGCCTTCAAAGTCCCTGAAATTAGAAAACAGATTAGGGAAAATCGGTACTGTTATAAAGGTCGACCTCGATTGAAAACTGGCCATGAACTCTTGAGGATTAGCTCAGAACTTGAACATAAGCTCGATGAGGTTTCTGTACCATTCCTCCTTCTTCATGGTGAAGATGATCGAGTTACGGACAGATTGGTTAGCAAACAACTCTACGACGAAGCAGTGAGCGATGATAAGACGTTGAAGATGTACCCGGGAATGTGGCATGGTTTGTTGTACGGCGAGACACCGGAGAATATAGATATTGTATTCTCAGACATCATCGACTGGTTGGAGAAGAGGAATGTGAAGTCGGAACTTGAGAAGAAGTATGAAAATGATCATCTCGGTGGCTTGGAATCCAAGCAACAATAAATTCATTAATTGTTATTCTAAAGAGTCCTTTTCGATTTGTTGGGAAAGAAAGTTGTGATATCGTTTTTAATCATATTGTAAATATTTATGAGTAATTTCAAAATTTATAGGTGAACGTTAATAAAGTTGCGTTTGAATGTTATTTGATGGTTAA

Coding sequence (CDS)

ATGGAGAATGTGACATACGAAGAGGAATTTATAGTGAATTCACGTGGGTTGAAGCTCTTCACGTGCAGTTGGGTTCCCAAGAATGATGAACCAAAAGCTCTGGTTTTTCTCTGCCATGGCTACGCCATGGAATGTAGCATCACCATGGACAGTAGTGCAAGGAGACTGGCAGAGGAAGGGTATGGAGTTTATGGGATTGATTACGAAGGACATGGGAAGTCCTCTGGGTTACAAGGCTATGTTTCAAGTTTCGATAAAGTAGTCGACGACTGTTCTTCGTTCTTCACTTCCATAACGGAAAAGAATGAAAATAGGGAGAAGAAAAGGTATTTGATGGGAGAGTCAATGGGAGGAGCGGTGGCTCTATTGGTACACAGAAAACAGCCTGATTATTGGGATGGAGCCATCTTAGTTGCTCCCATGTGTAAGATTGCTGAGGACATGAGACCAAATCCTTTGACGATAAGTTTTCTGACAAAGCTTTGCAAAATAATTCCAACCTGGAAAATTATTCCAACTCAAGATATTATTGATATTGCCTTCAAAGTCCCTGAAATTAGAAAACAGATTAGGGAAAATCGGTACTGTTATAAAGGTCGACCTCGATTGAAAACTGGCCATGAACTCTTGAGGATTAGCTCAGAACTTGAACATAAGCTCGATGAGGTTTCTGTACCATTCCTCCTTCTTCATGGTGAAGATGATCGAGTTACGGACAGATTGGTTAGCAAACAACTCTACGACGAAGCAGTGAGCGATGATAAGACGTTGAAGATGTACCCGGGAATGTGGCATGGTTTGTTGTACGGCGAGACACCGGAGAATATAGATATTGTATTCTCAGACATCATCGACTGGTTGGAGAAGAGGAATGTGAAGTCGGAACTTGAGAAGAAGTATGAAAATGATCATCTCGGTGGCTTGGAATCCAAGCAACAATAA

Protein sequence

MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGYGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAFKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRLVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKYENDHLGGLESKQQ
Homology
BLAST of CmoCh04G002480 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.7e-46
Identity = 98/274 (35.77%), Postives = 151/274 (55.11%), Query Frame = 0

Query: 18  KLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGYGVYGIDYEGHGKSSGL 77
           KLFT S++P + E K  V++ HGY  + S          +  GY V+  D  GHG+S G+
Sbjct: 50  KLFTQSFLPLDGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGI 109

Query: 78  QGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVALLVH-RKQPDYWDGAI 137
           + Y+   +KV     +FF  +   +  ++   +L GESMGG V LL++ + +P+ W G +
Sbjct: 110 RCYMGDMEKVAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLM 169

Query: 138 LVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAFKVPEIRKQIRENRYC 197
             AP+  I EDM+P+   +     L  +  TW  +P   ++  A K PE  K I  N   
Sbjct: 170 FSAPLFVIPEDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQR 229

Query: 198 YKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRLVSKQLYDEAVSDDKT 257
           Y G+PR+ T  ELLR +  ++    +V++P    HG  D VT    SK LY++A S DKT
Sbjct: 230 YTGKPRVGTMRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKT 289

Query: 258 LKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR 291
           LK+Y GM+H L+ GE  EN +IV  D+ +W++++
Sbjct: 290 LKIYEGMYHSLIQGEPDENAEIVLKDMREWIDEK 323

BLAST of CmoCh04G002480 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 3.0e-27
Identity = 87/290 (30.00%), Postives = 146/290 (50.34%), Query Frame = 0

Query: 2   ENVTYEE-EFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 61
           +NV Y++   +VN+ G  LF   W P +  PKAL+F+ HG    C    D  A+ L    
Sbjct: 12  QNVPYQDLPHLVNADGQYLFCRYWKP-SGTPKALIFVSHGAGEHCG-RYDELAQMLKRLD 71

Query: 62  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 121
             V+  D+ GHG+S G +  VS F   V D      ++  + +  E   +L+G SMGGA+
Sbjct: 72  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTV--QKDYPEVPVFLLGHSMGGAI 131

Query: 122 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 181
           ++L   ++P ++ G IL++P+  +A     + L +     L  ++P   +      + ++
Sbjct: 132 SILAAAERPTHFSGMILISPLI-LANPESASTLKVLAAKLLNFVLPNISLGRIDSSV-LS 191

Query: 182 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 241
               E+     +   C+ G  ++  G +LL   S +E  +  +++PFLLL G  DR+ D 
Sbjct: 192 RNKSEVDLYNSDPLICHAG-VKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDS 251

Query: 242 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR 291
             +  L + + S DKTLKMY G +H +L+ E PE  + V  +I  W+  R
Sbjct: 252 KGAYLLMESSPSQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHR 293

BLAST of CmoCh04G002480 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 90/295 (30.51%), Postives = 147/295 (49.83%), Query Frame = 0

Query: 2   ENVTYEE-EFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 61
           +NV Y++   +VN+ G  LF   W P +  PKAL+F+ HG    C    D  A  L    
Sbjct: 12  QNVPYQDLPHLVNADGQYLFCRYWKP-SGTPKALIFVSHGAGEHCG-RYDELAHMLKGLD 71

Query: 62  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 121
             V+  D+ GHG+S G +  VS F   V D      +I  + +  +   +L+G SMGGA+
Sbjct: 72  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI--QKDYPDVPIFLLGHSMGGAI 131

Query: 122 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 181
           ++LV  ++P Y+ G +L++P+      +  NP + S L  L   +  + ++P   +  I 
Sbjct: 132 SILVAAERPTYFSGMVLISPL------VLANPESASTLKVLAAKLLNF-VLPNMTLGRID 191

Query: 182 FKV-----PEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDD 241
             V      E+     +   C  G  ++  G +LL   + +E  +  +++PFLLL G  D
Sbjct: 192 SSVLSRNKSEVDLYNSDPLVCRAGL-KVCFGIQLLNAVARVERAMPRLTLPFLLLQGSAD 251

Query: 242 RVTDRLVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR 291
           R+ D   +  L + + S DKTLKMY G +H +L+ E PE  + V  ++  W+  R
Sbjct: 252 RLCDSKGAYLLMESSRSQDKTLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHR 293

BLAST of CmoCh04G002480 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 5.6e-26
Identity = 90/290 (31.03%), Postives = 147/290 (50.69%), Query Frame = 0

Query: 2   ENVTYEE-EFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 61
           +++ Y++   +VN+ G  LF   W P    PKAL+F+ HG A E S   +  AR L    
Sbjct: 12  QSIPYQDLPHLVNADGQYLFCRYWKP-TGTPKALIFVSHG-AGEHSGRYEELARMLMGLD 71

Query: 62  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 121
             V+  D+ GHG+S G +  VS F   V D      S+  + +      +L+G SMGGA+
Sbjct: 72  LLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM--QKDYPGLPVFLLGHSMGGAI 131

Query: 122 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 181
           A+L   ++P ++ G +L++P+  +A         +     L  ++P   + P    + ++
Sbjct: 132 AILTAAERPGHFAGMVLISPLV-LANPESATTFKVLAAKVLNLVLPNLSLGPIDSSV-LS 191

Query: 182 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 241
               E+     +   C  G  ++  G +LL   S +E  L +++VPFLLL G  DR+ D 
Sbjct: 192 RNKTEVDIYNSDPLICRAGL-KVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDS 251

Query: 242 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR 291
             +  L + A S DKTLK+Y G +H +L+ E PE  + VF +I  W+ +R
Sbjct: 252 KGAYLLMELAKSQDKTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQR 293

BLAST of CmoCh04G002480 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.2e-22
Identity = 73/289 (25.26%), Postives = 133/289 (46.02%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           M + T  E       G+++    W P  D P+ +V L HGYA E +      A+R    G
Sbjct: 1   MVSSTRSEHSFAGVGGVRIVYDVWTPDTD-PRGVVVLAHGYA-EHAGRYHHVAQRFGAAG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSI-TEKNENREKKRYLMGESMGGA 120
             VY +D+ GHG+S G + ++    + V+D   F T +    N++    R ++G SMGG 
Sbjct: 61  LLVYALDHRGHGRSGGKRVHLRDLSEFVED---FRTLVGIAANDHPTLPRIVLGHSMGGG 120

Query: 121 VALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDI-ID 180
           +      + P  +   +L  P     + +  +P+ ++    L K+ P    IP +++  D
Sbjct: 121 IVFAYGARYPGEYSAMVLSGPAVNAHDGV--SPVLVAVAKVLGKLAPG---IPVENLDAD 180

Query: 181 IAFKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVT 240
              + PE+    + +   + G+        L+ +   +  +   ++ P L++HG+ DR+ 
Sbjct: 181 AVSRDPEVVAAYKADPMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRLI 240

Query: 241 DRLVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWL 288
               S+ L D   S+D  LK+YPG++H +     PE   +V  D+  W+
Sbjct: 241 PVAGSRLLVDRVASEDVHLKVYPGLYHEVF--NEPEQ-KLVLDDVTSWI 276

BLAST of CmoCh04G002480 vs. ExPASy TrEMBL
Match: A0A6J1FPF0 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111447044 PE=4 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.3e-182
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300

Query: 301 ENDHLGGLESKQQ 314
           ENDHLGGLESKQQ
Sbjct: 301 ENDHLGGLESKQQ 313

BLAST of CmoCh04G002480 vs. ExPASy TrEMBL
Match: A0A6J1JZB9 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111489636 PE=4 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 5.4e-173
Identity = 297/313 (94.89%), Postives = 304/313 (97.12%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPK DEPKALVFLCHGYAMECSITMDSSARRLA EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKTDEPKALVFLCHGYAMECSITMDSSARRLAREG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKS GLQGYVSSFDKVVDDCSSFFTSI EK EN +KKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSFGLQGYVSSFDKVVDDCSSFFTSIAEKKENEKKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFL+KLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLSKLCKLIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQ+RENRYCYKGRPRLKTG+ELLRISSELEHKLDEVSVPFLLLHGEDDRVTD+
Sbjct: 181 FKVPEIRKQVRENRYCYKGRPRLKTGYELLRISSELEHKLDEVSVPFLLLHGEDDRVTDK 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMY GMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSE EKK+
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYSGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSEFEKKH 300

Query: 301 ENDHLGGLESKQQ 314
           ENDH GGLESKQQ
Sbjct: 301 ENDHHGGLESKQQ 313

BLAST of CmoCh04G002480 vs. ExPASy TrEMBL
Match: A0A0A0KS48 (Esterase/lipase/thioesterase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G179220 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.0e-156
Identity = 269/316 (85.13%), Postives = 293/316 (92.72%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           +ENV YEEE IVN RGLKLFTC+WVPKN+EPKAL+FLCHGYAMECSITMDSSARRLA+EG
Sbjct: 3   IENVRYEEEVIVNGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEG 62

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKSSGLQGYVSSFD VVDDCSSFFTSI+EK ENREKKRYLMGESMGGAV
Sbjct: 63  YGVYGIDYEGHGKSSGLQGYVSSFDNVVDDCSSFFTSISEKKENREKKRYLMGESMGGAV 122

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           AL++HRKQPD+WDGAILVAPMCKIA++MRPNPL IS LTKLCK+IPTWKIIPTQDIIDIA
Sbjct: 123 ALMIHRKQPDFWDGAILVAPMCKIADEMRPNPLVISLLTKLCKVIPTWKIIPTQDIIDIA 182

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FK P +RKQIREN YCYKGRPRL+TG+ELLRI+S LE KL EVS+PFLLLHGEDDRVTD+
Sbjct: 183 FKQPHVRKQIRENAYCYKGRPRLRTGYELLRITSLLETKLHEVSLPFLLLHGEDDRVTDK 242

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSEL 300
           LVSKQLYD+A SDDKTL MYPGMWHGLLYGETPENIDIVFSDII WL+KR    N+KSEL
Sbjct: 243 LVSKQLYDDAASDDKTLNMYPGMWHGLLYGETPENIDIVFSDIIGWLDKRSIVENLKSEL 302

Query: 301 EKKYENDHLGGLESKQ 313
           E+KYEND  G LE+K+
Sbjct: 303 ERKYEND--GLLEAKK 316

BLAST of CmoCh04G002480 vs. ExPASy TrEMBL
Match: A0A6J1CCN8 (caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111010007 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.7e-147
Identity = 254/317 (80.13%), Postives = 287/317 (90.54%), Query Frame = 0

Query: 2   ENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGY 61
           ENV YEEEF+ ++RGLKLFTCSW+P  +EPKAL+F+CHGYAMECSITM+S+ARRLA+EGY
Sbjct: 6   ENVKYEEEFVESARGLKLFTCSWIPVKEEPKALIFICHGYAMECSITMNSTARRLAKEGY 65

Query: 62  GVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVA 121
           G+YG+DYEGHGKSSGLQG+VSSFD VV+DCS+FFTSITEK EN +KKRYL+GESMGGAVA
Sbjct: 66  GIYGMDYEGHGKSSGLQGFVSSFDNVVEDCSTFFTSITEKKENGKKKRYLLGESMGGAVA 125

Query: 122 LLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAF 181
           LL+HRKQPDYWDGAILVAPMCKIA+DMRPNP+ IS LTKL K+IPTWK+IPT+DIIDIAF
Sbjct: 126 LLIHRKQPDYWDGAILVAPMCKIAKDMRPNPMMISMLTKLSKLIPTWKLIPTEDIIDIAF 185

Query: 182 KVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRL 241
           KVPE+RKQIREN YCYKGRPRLKTG+ELLR SSELE  L EV +PFL+LHGEDDRVTD+L
Sbjct: 186 KVPEVRKQIRENSYCYKGRPRLKTGYELLRTSSELERNLKEVCLPFLVLHGEDDRVTDKL 245

Query: 242 VSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNV-----KSEL 301
           VSKQLYDEA SDDKTLKMYPGMWHGLLYGETPENIDIVF+DI  WLE+R+V     +SE 
Sbjct: 246 VSKQLYDEASSDDKTLKMYPGMWHGLLYGETPENIDIVFADITSWLEERSVANGDSRSEH 305

Query: 302 EKKYENDHLGGLESKQQ 314
           E K END  G LE+KQQ
Sbjct: 306 EIKREND--GLLEAKQQ 320

BLAST of CmoCh04G002480 vs. ExPASy TrEMBL
Match: A0A5A7V5I5 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00130 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.9e-142
Identity = 247/308 (80.19%), Postives = 274/308 (88.96%), Query Frame = 0

Query: 2   ENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGY 61
           ENV YEEE I N RGLKLFTC+WVPKN+EPKAL+FLCHGYAMECSITMDSSARRLA+EGY
Sbjct: 4   ENVRYEEEVIENGRGLKLFTCNWVPKNEEPKALIFLCHGYAMECSITMDSSARRLAKEGY 63

Query: 62  GVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVA 121
           GVYGIDYEGHGKSSGLQGYVSSFD VV+DCSSFFTSI+              ESMGGAVA
Sbjct: 64  GVYGIDYEGHGKSSGLQGYVSSFDNVVEDCSSFFTSIS--------------ESMGGAVA 123

Query: 122 LLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAF 181
           L++H KQPD+WDGAILVAPMCKIA++MRPNPL ISFLTKLCKIIPTWKIIPTQD+ID AF
Sbjct: 124 LMIHIKQPDFWDGAILVAPMCKIADEMRPNPLVISFLTKLCKIIPTWKIIPTQDVIDFAF 183

Query: 182 KVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRL 241
           K+P++RKQIREN YCYKGRPRLKTG+ELLR+++ LE+KL+EVS+PFLLLHGEDDRVTD+L
Sbjct: 184 KLPQVRKQIRENPYCYKGRPRLKTGYELLRVTALLENKLNEVSLPFLLLHGEDDRVTDKL 243

Query: 242 VSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSELE 301
           VSKQLYDEA SDDKTL MY GMWHGLLYGETPEN+DIVFSDII WL+KR    N+KSELE
Sbjct: 244 VSKQLYDEAASDDKTLNMYQGMWHGLLYGETPENVDIVFSDIIGWLDKRSIDENLKSELE 297

Query: 302 KKYENDHL 306
           +K END L
Sbjct: 304 RKKENDGL 297

BLAST of CmoCh04G002480 vs. NCBI nr
Match: XP_022941777.1 (caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941778.1 caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941779.1 caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 646.4 bits (1666), Expect = 1.3e-181
Identity = 313/313 (100.00%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300

Query: 301 ENDHLGGLESKQQ 314
           ENDHLGGLESKQQ
Sbjct: 301 ENDHLGGLESKQQ 313

BLAST of CmoCh04G002480 vs. NCBI nr
Match: KAG6600077.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030746.1 Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 644.4 bits (1661), Expect = 5.0e-181
Identity = 311/313 (99.36%), Postives = 313/313 (100.00%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITM+SSARRLAEEG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMESSARRLAEEG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDE+SVPFLLLHGEDDRVTDR
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEISVPFLLLHGEDDRVTDR 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300

Query: 301 ENDHLGGLESKQQ 314
           ENDHLGGLESKQQ
Sbjct: 301 ENDHLGGLESKQQ 313

BLAST of CmoCh04G002480 vs. NCBI nr
Match: XP_023547317.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 620.2 bits (1598), Expect = 1.0e-173
Identity = 299/313 (95.53%), Postives = 307/313 (98.08%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPKN+EPKALVFLCHGYAMECSITMDSSARRLA+EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNEEPKALVFLCHGYAMECSITMDSSARRLAKEG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYG+DYEGHGKSSGLQGYVSSF+ VVDDCSSFFTSI EK ENREKKRYLMGESMGGAV
Sbjct: 61  YGVYGVDYEGHGKSSGLQGYVSSFNNVVDDCSSFFTSIAEKKENREKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           A+LVHRKQP+YWDGAILVAPMCKIAEDMRPN LTISFLTKLCKIIPTWKIIPTQDIIDIA
Sbjct: 121 AVLVHRKQPEYWDGAILVAPMCKIAEDMRPNALTISFLTKLCKIIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQIRENRYCYKGRPRLKTG+ELLRISSELEHKLDEVSVPFLLLHGEDDRVTD+
Sbjct: 181 FKVPEIRKQIRENRYCYKGRPRLKTGYELLRISSELEHKLDEVSVPFLLLHGEDDRVTDK 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300

Query: 301 ENDHLGGLESKQQ 314
           END  GGLESKQQ
Sbjct: 301 ENDRRGGLESKQQ 313

BLAST of CmoCh04G002480 vs. NCBI nr
Match: XP_022993725.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 616.7 bits (1589), Expect = 1.1e-172
Identity = 297/313 (94.89%), Postives = 304/313 (97.12%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           MENVTYEEEFIVNSRGLKLFTCSWVPK DEPKALVFLCHGYAMECSITMDSSARRLA EG
Sbjct: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKTDEPKALVFLCHGYAMECSITMDSSARRLAREG 60

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKS GLQGYVSSFDKVVDDCSSFFTSI EK EN +KKRYLMGESMGGAV
Sbjct: 61  YGVYGIDYEGHGKSFGLQGYVSSFDKVVDDCSSFFTSIAEKKENEKKKRYLMGESMGGAV 120

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFL+KLCK+IPTWKIIPTQDIIDIA
Sbjct: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLSKLCKLIPTWKIIPTQDIIDIA 180

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQ+RENRYCYKGRPRLKTG+ELLRISSELEHKLDEVSVPFLLLHGEDDRVTD+
Sbjct: 181 FKVPEIRKQVRENRYCYKGRPRLKTGYELLRISSELEHKLDEVSVPFLLLHGEDDRVTDK 240

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSELEKKY 300
           LVSKQLYDEAVSDDKTLKMY GMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSE EKK+
Sbjct: 241 LVSKQLYDEAVSDDKTLKMYSGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSEFEKKH 300

Query: 301 ENDHLGGLESKQQ 314
           ENDH GGLESKQQ
Sbjct: 301 ENDHHGGLESKQQ 313

BLAST of CmoCh04G002480 vs. NCBI nr
Match: XP_038891440.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 272/319 (85.27%), Postives = 298/319 (93.42%), Query Frame = 0

Query: 1   MENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEG 60
           ++NV YEEE IVN+ GLKLFTC+W+PKN+E KAL+F+CHGYAMECSITM+S+ARRLA+EG
Sbjct: 5   IDNVRYEEEVIVNAHGLKLFTCNWIPKNEESKALIFICHGYAMECSITMNSTARRLAKEG 64

Query: 61  YGVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAV 120
           YGVYGIDYEGHGKS GLQGYVSSFD VV+DCSSFFTSI+EK ENREKKRYLMGESMGGAV
Sbjct: 65  YGVYGIDYEGHGKSGGLQGYVSSFDNVVEDCSSFFTSISEKKENREKKRYLMGESMGGAV 124

Query: 121 ALLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIA 180
           ALL+HRKQPD+WDGAILVAPMCKIA+DMRP+PL IS LTKLCKIIPTWKIIPTQD+IDIA
Sbjct: 125 ALLIHRKQPDFWDGAILVAPMCKIADDMRPHPLVISVLTKLCKIIPTWKIIPTQDVIDIA 184

Query: 181 FKVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDR 240
           FKVPEIRKQIREN YCYKGRPRLKTG+ELLRISS+LEHKL+EVS+PFLLLHGEDDRVTD+
Sbjct: 185 FKVPEIRKQIRENPYCYKGRPRLKTGYELLRISSDLEHKLNEVSLPFLLLHGEDDRVTDK 244

Query: 241 LVSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR------NVKS 300
           LVSKQLYDEA+SDDKTLKMYPGMWHGLLYGETPEN+DIVFSDII WLEKR      N KS
Sbjct: 245 LVSKQLYDEALSDDKTLKMYPGMWHGLLYGETPENMDIVFSDIISWLEKRSIVAYENSKS 304

Query: 301 ELEKKYENDHLGGLESKQQ 314
           ELEKKYEND  G LE+KQQ
Sbjct: 305 ELEKKYEND--GLLEAKQQ 321

BLAST of CmoCh04G002480 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 454.1 bits (1167), Expect = 8.9e-128
Identity = 210/312 (67.31%), Postives = 258/312 (82.69%), Query Frame = 0

Query: 2   ENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGY 61
           EN+ YEE FI N+RG+KLFTC WVP   EPKALVF+CHGYAMECSITM+S+ARRL + G+
Sbjct: 6   ENIKYEESFIKNTRGMKLFTCKWVPAKQEPKALVFICHGYAMECSITMNSTARRLVKAGF 65

Query: 62  GVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVA 121
            VYGIDYEGHGKS GL  YV +FD +VDD S+ +TSI EK EN+ K R+L+GESMGGAV 
Sbjct: 66  AVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVL 125

Query: 122 LLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAF 181
           LL+HRK+P +WDGA+LVAPMCKIAE+M+P+PL IS L KL  +IP+WKIIP QDII+ AF
Sbjct: 126 LLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAF 185

Query: 182 KVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRL 241
           K PEIRKQ+REN YCYKGRPRLKT +ELLR+S++LE +L+EVS+PF++LHGEDD+VTD+ 
Sbjct: 186 KQPEIRKQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDKA 245

Query: 242 VSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR------NVKSE 301
           VS+QLY+ A S DKT K+YPGMWHGLLYGETPENI+ VF+DII WL+K+        +SE
Sbjct: 246 VSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLDKKVADESGGFESE 305

Query: 302 LEKKYENDHLGG 308
           L++K +   L G
Sbjct: 306 LKRKNDGIPLKG 317

BLAST of CmoCh04G002480 vs. TAIR 10
Match: AT2G39410.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.2e-124
Identity = 206/308 (66.88%), Postives = 255/308 (82.79%), Query Frame = 0

Query: 2   ENVTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGY 61
           E++ YEE FI N+RG KLFTC W+P N EP+ALVFLCHGY MECSITM+S+ARRL + G+
Sbjct: 6   EDIKYEESFIKNTRGFKLFTCRWLPTNREPRALVFLCHGYGMECSITMNSTARRLVKAGF 65

Query: 62  GVYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVA 121
            VYG+DYEGHGKS GL  Y+S+FD++VDD S+ +T+I E+ EN+ K R+++GESMGGAV 
Sbjct: 66  AVYGMDYEGHGKSDGLSAYISNFDRLVDDVSTHYTAICEREENKWKMRFMLGESMGGAVV 125

Query: 122 LLVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAF 181
           LL+ RK PD+WDGAILVAPMCKIAE+M+P+P  IS LTKL  IIP WKIIP+QDII+I++
Sbjct: 126 LLLGRKNPDFWDGAILVAPMCKIAEEMKPSPFVISILTKLISIIPKWKIIPSQDIIEISY 185

Query: 182 KVPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRL 241
           K PEIRKQ+REN  C KGRPRLKT +ELLRIS++LE +L EVS+PFL+LHG+DD+VTD+ 
Sbjct: 186 KEPEIRKQVRENPLCSKGRPRLKTAYELLRISNDLEKRLQEVSLPFLVLHGDDDKVTDKA 245

Query: 242 VSKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSELE 301
           VS++LY  A+S DKTLK+YPGMWHGLL GETPENI+IVF+D+I WLEKR    N + E E
Sbjct: 246 VSQELYKVALSADKTLKLYPGMWHGLLTGETPENIEIVFADVISWLEKRSDYGNDRFESE 305

Query: 302 KKYENDHL 306
            K  ND L
Sbjct: 306 LKQRNDRL 313

BLAST of CmoCh04G002480 vs. TAIR 10
Match: AT3G55180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 424.5 bits (1090), Expect = 7.5e-119
Identity = 197/305 (64.59%), Postives = 247/305 (80.98%), Query Frame = 0

Query: 4   VTYEEEFIVNSRGLKLFTCSW-VPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGYG 63
           V Y+E+++ NSRG++LFTCSW   +  EPKAL+FLCHGYAME SITM S+A RLA  G+ 
Sbjct: 2   VMYKEDYVSNSRGIQLFTCSWKQEEQQEPKALIFLCHGYAMESSITMSSTAVRLANAGFS 61

Query: 64  VYGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVAL 123
           VYG+DYEGHGKS GL GYV  FD +V D SS ++SI E  EN+ K R+LMGESMGGAV L
Sbjct: 62  VYGMDYEGHGKSGGLNGYVKKFDDLVQDVSSHYSSICELEENKGKMRFLMGESMGGAVVL 121

Query: 124 LVHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAFK 183
           L+ RK+P++WDGA+LVAPMCK+AED++P+P+ ISFLTKL + IPTWKI+P+ DIID+AFK
Sbjct: 122 LLERKKPNFWDGAVLVAPMCKLAEDIKPHPMVISFLTKLTRFIPTWKIVPSNDIIDVAFK 181

Query: 184 VPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRLV 243
              IRKQ+R+N YCYKGRPRLKT H+LL +S +LE  LD+VS+PF++LHGEDD+VTD+ V
Sbjct: 182 ETHIRKQVRDNEYCYKGRPRLKTAHQLLMVSLDLEKNLDQVSMPFIVLHGEDDKVTDKNV 241

Query: 244 SKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR----NVKSELEK 303
           SK LY+ A S DKT K+YP MWHGLLYGE+PEN++IVFSDII WL++R    N K E E 
Sbjct: 242 SKLLYEVASSSDKTFKLYPNMWHGLLYGESPENLEIVFSDIISWLKERASVTNQKLETEL 301

BLAST of CmoCh04G002480 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 419.9 bits (1078), Expect = 1.9e-117
Identity = 193/310 (62.26%), Postives = 246/310 (79.35%), Query Frame = 0

Query: 4   VTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGYGV 63
           V YEE+F++NSRG+KLFTC W P   EPKAL+FLCHGYAME SITM+S+A RLA  G+ V
Sbjct: 2   VMYEEDFVLNSRGMKLFTCVWKPVKQEPKALLFLCHGYAMESSITMNSAATRLANAGFAV 61

Query: 64  YGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVALL 123
           YG+DYEGHGKS GL GY+S+FD +VDD S+ +++I E+ EN+ K R+L+GESMGGAV LL
Sbjct: 62  YGMDYEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVLL 121

Query: 124 VHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWKIIPTQDIIDIAFKV 183
           + RK+PD+WDGA+LVAPMCK+A++++P+P+ IS L KL K IPTWKI+P  DIIDIA K 
Sbjct: 122 LARKKPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIKE 181

Query: 184 PEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRLVS 243
           P IR Q+REN+YCYKGRPRL T ++LL +S +LE  L +VS+PF++LHGEDD+VTD+ +S
Sbjct: 182 PHIRNQVRENKYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKSIS 241

Query: 244 KQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKRNVKSE--LEKKYE 303
           K LY+ A S DKT K+YP MWH LLYGET EN +IVF DII+WLE R   S   LE + +
Sbjct: 242 KMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRATDSNGGLESQLK 301

Query: 304 NDHLGGLESK 312
           + H G L+ K
Sbjct: 302 HKHDGFLKHK 311

BLAST of CmoCh04G002480 vs. TAIR 10
Match: AT3G55190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 3.8e-102
Identity = 166/288 (57.64%), Postives = 225/288 (78.12%), Query Frame = 0

Query: 4   VTYEEEFIVNSRGLKLFTCSWVPKNDEPKALVFLCHGYAMECSITMDSSARRLAEEGYGV 63
           V Y EEFI NSRG++L TC W P N EP+AL+F CHGYA++CS T    A + A+EG+ V
Sbjct: 8   VGYSEEFIENSRGMQLLTCKWFPVNQEPRALIFFCHGYAIDCSTTFKDIAPKFAKEGFAV 67

Query: 64  YGIDYEGHGKSSGLQGYVSSFDKVVDDCSSFFTSITEKNENREKKRYLMGESMGGAVALL 123
           +GI+YEGHG+SSGL  Y+ +FD ++DD SS F+ I+E  +N +KKR+LMGESMGGAV LL
Sbjct: 68  HGIEYEGHGRSSGLSVYIDNFDLLIDDVSSHFSKISEMGDNTKKKRFLMGESMGGAVVLL 127

Query: 124 VHRKQPDYWDGAILVAPMCKIAEDMRPNPLTISFLTKLCKIIPTWK-IIPTQDIIDIAFK 183
           +HRK+P++WDG IL+APMCKIAE+M+P+ + IS +  +  +IP+WK II   DI++ A K
Sbjct: 128 LHRKKPEFWDGGILIAPMCKIAEEMKPSRMVISMINMVTNLIPSWKSIIHGPDILNSAIK 187

Query: 184 VPEIRKQIRENRYCYKGRPRLKTGHELLRISSELEHKLDEVSVPFLLLHGEDDRVTDRLV 243
           +PE R +IR N  CY G PR+KT  EL RIS +LE++L+EV++PF++LHGEDD+VTD+  
Sbjct: 188 LPEKRHEIRTNPNCYNGWPRMKTMSELFRISLDLENRLNEVTMPFIVLHGEDDKVTDKGG 247

Query: 244 SKQLYDEAVSDDKTLKMYPGMWHGLLYGETPENIDIVFSDIIDWLEKR 291
           SK LY+ A+S+DKTLK+YP MWH LL+GE PEN +IVF+DI+ W++ R
Sbjct: 248 SKLLYEVALSNDKTLKLYPEMWHSLLFGEPPENSEIVFNDIVQWMQTR 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9421.7e-4635.77Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1[more]
Q8R4313.0e-2730.00Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1[more]
O356781.5e-2630.51Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1[more]
Q996855.6e-2631.03Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2[more]
A0QNZ72.2e-2225.26Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(... [more]
Match NameE-valueIdentityDescription
A0A6J1FPF06.3e-182100.00caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111447044 PE... [more]
A0A6J1JZB95.4e-17394.89caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111489636 PE=4... [more]
A0A0A0KS486.0e-15685.13Esterase/lipase/thioesterase family protein OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A6J1CCN81.7e-14780.13caffeoylshikimate esterase-like OS=Momordica charantia OX=3673 GN=LOC111010007 P... [more]
A0A5A7V5I52.9e-14280.19Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
Match NameE-valueIdentityDescription
XP_022941777.11.3e-181100.00caffeoylshikimate esterase-like [Cucurbita moschata] >XP_022941778.1 caffeoylshi... [more]
KAG6600077.15.0e-18199.36Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_023547317.11.0e-17395.53caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo][more]
XP_022993725.11.1e-17294.89caffeoylshikimate esterase-like [Cucurbita maxima][more]
XP_038891440.11.7e-15785.27caffeoylshikimate esterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G39420.18.9e-12867.31alpha/beta-Hydrolases superfamily protein [more]
AT2G39410.21.2e-12466.88alpha/beta-Hydrolases superfamily protein [more]
AT3G55180.17.5e-11964.59alpha/beta-Hydrolases superfamily protein [more]
AT2G39400.11.9e-11762.26alpha/beta-Hydrolases superfamily protein [more]
AT3G55190.13.8e-10257.64alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 111..124
score: 50.0
coord: 227..241
score: 33.1
coord: 60..75
score: 36.21
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 2..295
e-value: 2.5E-62
score: 212.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 3..290
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 30..268
e-value: 4.5E-66
score: 222.5
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 6..304
NoneNo IPR availablePANTHERPTHR11614:SF176ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G002480.1CmoCh04G002480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016298 lipase activity