CmoCh03G008910 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G008910
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionRhamnogalacturonan endolyase
LocationCmo_Chr03: 6907147 .. 6911511 (-)
RNA-Seq ExpressionCmoCh03G008910
SyntenyCmoCh03G008910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGAGAGGCAAGGCAAGAAGGCAGCACCCATACTGGACATGGAAACTTGGGTTCTCAGCAATGCCGCTCCTTTTCTTATTCTTCAGTTTAACCCTCGCCCACAACAATTCCTCCCAACGGTTACGTTCTTCTTCTTCTTCTTCTTCTTCTTTGATTTTTGGTTGTTGTTTTGATTTTGATTTTGATTTTGATTTTGATTTTGATTTTGATTAGTTGTAATCAATTGCAGAAGAATGGTAAGAAAGTTGGTGAAGAACAATGAATCTATGCTGTCGCGCGATGTAACGTTAGAATGGGAGAATCGTGGACAGGTGATTATGTACTAACCAAATTCTTCTTTTTACGCCAATATATTTTATACCTTATTTGACGCTAAATAAATTTCAAAATAAGGGAAGTAATTATTTTTTGGTTTCAGGTAGTAATGGACAATGGGTTAGTTCGAATCACTTTATCCAGACCAGAAGGCAACGTTATTGCCCTAAAATACAATGCTCTTGATAACCTACTTGAAATTCGCAACCCACAATATAACAGAGGGTAATTCTCTCCATTTTCTTATATATATTTTTTAATTTATTCACTCTTAAATTAACTAGACATTTATTATAATTTAAATTCTTTTAAAAAATTGAAACTACCCATGAGTTCCGGCCTCTATTAAAAAGTAGAACAAAACATTGTTTACAATCATGTTTAAACTTCTTTCCATAAACATACGGTTTAAAAAATCTTGAGAAAATTCGAAAGGAAAAGTCTAAAAAGAACAATATCTACTCGTAGTGAACTTGGACGGTTATAAATGATATGGATTGTGGCTTAGGTTGTTACAAATGTGGTATGGATTGTGAGATCTCACATTAGTATGGATTATGACCGACACGTCTTAAAAACTTTAAGGGAACCATGGAAAGGGAAAGCCTGAAGACAATTGCTAGAAGTGGGTTTAGGTTGTTACACCAACTAACTCGTTTATTTAATATAATTATTTATATGAGAAATTATAATTAACCTTTTTAATGTTATTATGGTTGACATGTTGTGTAGATATTGGGACGTGGTTTGGAACCGCCCAAATAGAGGTAGTGCCACTGACAGGTAACTACTGAATTTATATATTATATATATTGAATTATATTCATTTTTTTACATTATTGGTTATAGTTTAAAAAAAAAACAATGAAAATATTCTATAATTTCACTTTTAATGATGCCTTTACAGAGTAAGGGCGACGAATTTCAAAGTGATTACGGCCACCGCCGACCAAATAGAGCTTTCATTCACCAAAACATGGAATCCTAATTCCCACTCTTCCTGCCCTTTGAACATAGACAAAAGGTGCTCCTCTCTCCTTGTTTCTAAAATAATTATGAACTTTTTTTTTTCCAAGTTAAATGAATGATATTAGTTAAGTTACAATTTTTAGGTATATTCTCCGACGGGGCGATTCTGGGTTCTATGCATATGCCATATTTGAACGCCTTGATGGATGGCCTCAAATTGACGTTGATCAGATTCGAATTGTCTTCAAAATTCTAGGGAAAAAGTTAGTGTTGTGCTGTTAAATTTTCTCCTTCTAATTTGAAATATAATTTATATTTATGCTTTGCTGTATAATCAACGGTCAGATTTCATTACATGGTGGTATCGGACGACAGGCAGAGGATCATGCCAACGGCAAGGGACCGTGAGACCGGGCATCCTCTGGCTTATCGGGAGGCGGTTCTCTTAACCCAACCCATCAATTCTAAACTCAAAGGGGAGGTGGGAATAATTAAATTTTGTGAAGTATAATTTAGGATAATAATGTGTGATTTTAATTGAGGGAATGATTGGGTGTGGTGCAGGTGGACGATAAGTACCAGTACTCCCTAGAGAATATGGAGAATAAGGTTCATGGATGGGAGTCGATGAACCCACACGTGGGGTTCTGGATGATCACGCCCAGTGATGAGTTCCGCACGGCGGGGCCCGTCAAGCAAGATCTTACTTCCCACGTTGGCCCCACCACCCTCTCCGTAAGCCCTCCCTACACACCCGTTTCCTAATTTTTTGTTTTTCCATTCGGTCCAATCTTTATAAATAAAATCAACCTTTTCCTTTATTTTCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATGTTCGTGAGTACGCATTACGCGGGTAAAGAAGTTTCCATGAGATTTGGAGTGGGAGAGGCATGGAAGAAGGTATTCGGACCGGTCTTCATTTATCTAAACTCGGATCCGGTCGGTTCTGGACCCGGTTCACTTTGGGAAGATGCTAAAAACCAGGTTTTTTTTTTTAATTTTTATGATCAGACCTTTATTTATAGGAAAAAAGGGGAAACTGAAAAATGGGTATAATTTAAAATCAGATGAGGATAGAGACAAGTAAGTGGCCATACAGCTTTGTTGGATCCACAGATTTTCCATCCTCAAACCAAAGAGGAACCGTAAGCGGTCGGTTATTAGTCCGAGATAGGTTCGTCTTTCTCCCCTTTACTTCCTTACGTTGTATTTTTCATGTCTCTGTCCTTTTAAGGTTCAACAATAAAATCCTGTCGACGTCATTTTTATTGGCAGACATGCTAAGAAGTGGTTGATGTGGGGCGATTCTGCGTATGTTGGCCTTGCAGCTCCCGGGGAATTGGGGTCTTGGCAAACAGAAAGCAAGGTCACATATAAATGGATAAATTATTGTATTTTCCATTAGATGGAAGTAATTGACCATTGTCTTCATTTTCATTGACAGGGATATCAGTTTTGGACACGGGCAGATGGGCAAGGATATTTCTCAATTAACAACATTAGGCCTGGAAACTACAATCTATACGCGTGGGTACCTGGTGTCATCGGTGATTACAAGTACGCCAATGACATCACTGTCACGTTGGGGAGTACCACCCGCCTCGGCTTACTCATATACGACCCTCCAAGACAAGGCCCAACTCTGTGGGAGATTGGCGTCCCAGACCGGACAGCCGCCGAGTTCTATATACCGGATCCCCAGCCCACCCTCATGAATCGCTTTTACAACAATTCTGATATCCATACCCCAGATAAGTTTCGACAGTACGGTCTTTGGGAGCGCTACGCCGAATTGTTTCCCCATCAAGATCTAGTCTACAACGTCGCCACCGATGACTACCGCCGACATTGGTTCTTCGCCCATGTCACCAGAGCCACCCGTTTCGGTACCTACCAAGCAACCACGTGGCAGATTGTGTTTCGTCTTCAAGGGCCCAAACAGAGCGGGAACTATATGCTTCGGTTGGCCTTGGCTTCCACTACCGAGGCGGTGGTCGAAATCCGGTTCAACAACCCGTTGGCCAGAGGGGCTCATTTCTCGACCGGGTATTGGGCCTCAGGAAGGGATAACGCCATTGCTAGACATGGGATTCATGGGCTTTATTGGTTATTCAACTTCAAGGTTCCAAGTGGATATTTGGTCGAGGATGAAAACATTATCTATCTTACACAGAGACGACATATGGGACCCTTTCAAGGAGTGATGTACGATTACATACGGTTCGAAGGCGTACAATAA

mRNA sequence

ATGGAGAAGAGAGGCAAGGCAAGAAGGCAGCACCCATACTGGACATGGAAACTTGGGTTCTCAGCAATGCCGCTCCTTTTCTTATTCTTCAGTTTAACCCTCGCCCACAACAATTCCTCCCAACGGTTACTTGTAATCAATTGCAGAAGAATGGTAAGAAAGTTGGTGAAGAACAATGAATCTATGCTGTCGCGCGATGTAACGTTAGAATGGGAGAATCGTGGACAGGTAGTAATGGACAATGGGTTAGTTCGAATCACTTTATCCAGACCAGAAGGCAACGTTATTGCCCTAAAATACAATGCTCTTGATAACCTACTTGAAATTCGCAACCCACAATATAACAGAGGATATTGGGACGTGGTTTGGAACCGCCCAAATAGAGGTAGTGCCACTGACAGAGTAAGGGCGACGAATTTCAAAGTGATTACGGCCACCGCCGACCAAATAGAGCTTTCATTCACCAAAACATGGAATCCTAATTCCCACTCTTCCTGCCCTTTGAACATAGACAAAAGGTATATTCTCCGACGGGGCGATTCTGGGTTCTATGCATATGCCATATTTGAACGCCTTGATGGATGGCCTCAAATTGACGTTGATCAGATTCGAATTGTCTTCAAAATTCTAGGGAAAAAATTTCATTACATGGTGGTATCGGACGACAGGCAGAGGATCATGCCAACGGCAAGGGACCGTGAGACCGGGCATCCTCTGGCTTATCGGGAGGCGGTTCTCTTAACCCAACCCATCAATTCTAAACTCAAAGGGGAGGTGGACGATAAGTACCAGTACTCCCTAGAGAATATGGAGAATAAGGTTCATGGATGGGAGTCGATGAACCCACACGTGGGGTTCTGGATGATCACGCCCAGTGATGAGTTCCGCACGGCGGGGCCCGTCAAGCAAGATCTTACTTCCCACGTTGGCCCCACCACCCTCTCCATGTTCGTGAGTACGCATTACGCGGGTAAAGAAGTTTCCATGAGATTTGGAGTGGGAGAGGCATGGAAGAAGGTATTCGGACCGGTCTTCATTTATCTAAACTCGGATCCGGTCGGTTCTGGACCCGGTTCACTTTGGGAAGATGCTAAAAACCAGATGAGGATAGAGACAAGTAAGTGGCCATACAGCTTTGTTGGATCCACAGATTTTCCATCCTCAAACCAAAGAGGAACCGTAAGCGGTCGGTTATTAGTCCGAGATAGACATGCTAAGAAGTGGTTGATGTGGGGCGATTCTGCGTATGTTGGCCTTGCAGCTCCCGGGGAATTGGGGTCTTGGCAAACAGAAAGCAAGGGATATCAGTTTTGGACACGGGCAGATGGGCAAGGATATTTCTCAATTAACAACATTAGGCCTGGAAACTACAATCTATACGCGTGGGTACCTGGTGTCATCGGTGATTACAAGTACGCCAATGACATCACTGTCACGTTGGGGAGTACCACCCGCCTCGGCTTACTCATATACGACCCTCCAAGACAAGGCCCAACTCTGTGGGAGATTGGCGTCCCAGACCGGACAGCCGCCGAGTTCTATATACCGGATCCCCAGCCCACCCTCATGAATCGCTTTTACAACAATTCTGATATCCATACCCCAGATAAGTTTCGACAGTACGGTCTTTGGGAGCGCTACGCCGAATTGTTTCCCCATCAAGATCTAGTCTACAACGTCGCCACCGATGACTACCGCCGACATTGGTTCTTCGCCCATGTCACCAGAGCCACCCGTTTCGGTACCTACCAAGCAACCACGTGGCAGATTGTGTTTCGTCTTCAAGGGCCCAAACAGAGCGGGAACTATATGCTTCGGTTGGCCTTGGCTTCCACTACCGAGGCGGTGGTCGAAATCCGGTTCAACAACCCGTTGGCCAGAGGGGCTCATTTCTCGACCGGGTATTGGGCCTCAGGAAGGGATAACGCCATTGCTAGACATGGGATTCATGGGCTTTATTGGTTATTCAACTTCAAGGTTCCAAGTGGATATTTGGTCGAGGATGAAAACATTATCTATCTTACACAGAGACGACATATGGGACCCTTTCAAGGAGTGATGTACGATTACATACGGTTCGAAGGCGTACAATAA

Coding sequence (CDS)

ATGGAGAAGAGAGGCAAGGCAAGAAGGCAGCACCCATACTGGACATGGAAACTTGGGTTCTCAGCAATGCCGCTCCTTTTCTTATTCTTCAGTTTAACCCTCGCCCACAACAATTCCTCCCAACGGTTACTTGTAATCAATTGCAGAAGAATGGTAAGAAAGTTGGTGAAGAACAATGAATCTATGCTGTCGCGCGATGTAACGTTAGAATGGGAGAATCGTGGACAGGTAGTAATGGACAATGGGTTAGTTCGAATCACTTTATCCAGACCAGAAGGCAACGTTATTGCCCTAAAATACAATGCTCTTGATAACCTACTTGAAATTCGCAACCCACAATATAACAGAGGATATTGGGACGTGGTTTGGAACCGCCCAAATAGAGGTAGTGCCACTGACAGAGTAAGGGCGACGAATTTCAAAGTGATTACGGCCACCGCCGACCAAATAGAGCTTTCATTCACCAAAACATGGAATCCTAATTCCCACTCTTCCTGCCCTTTGAACATAGACAAAAGGTATATTCTCCGACGGGGCGATTCTGGGTTCTATGCATATGCCATATTTGAACGCCTTGATGGATGGCCTCAAATTGACGTTGATCAGATTCGAATTGTCTTCAAAATTCTAGGGAAAAAATTTCATTACATGGTGGTATCGGACGACAGGCAGAGGATCATGCCAACGGCAAGGGACCGTGAGACCGGGCATCCTCTGGCTTATCGGGAGGCGGTTCTCTTAACCCAACCCATCAATTCTAAACTCAAAGGGGAGGTGGACGATAAGTACCAGTACTCCCTAGAGAATATGGAGAATAAGGTTCATGGATGGGAGTCGATGAACCCACACGTGGGGTTCTGGATGATCACGCCCAGTGATGAGTTCCGCACGGCGGGGCCCGTCAAGCAAGATCTTACTTCCCACGTTGGCCCCACCACCCTCTCCATGTTCGTGAGTACGCATTACGCGGGTAAAGAAGTTTCCATGAGATTTGGAGTGGGAGAGGCATGGAAGAAGGTATTCGGACCGGTCTTCATTTATCTAAACTCGGATCCGGTCGGTTCTGGACCCGGTTCACTTTGGGAAGATGCTAAAAACCAGATGAGGATAGAGACAAGTAAGTGGCCATACAGCTTTGTTGGATCCACAGATTTTCCATCCTCAAACCAAAGAGGAACCGTAAGCGGTCGGTTATTAGTCCGAGATAGACATGCTAAGAAGTGGTTGATGTGGGGCGATTCTGCGTATGTTGGCCTTGCAGCTCCCGGGGAATTGGGGTCTTGGCAAACAGAAAGCAAGGGATATCAGTTTTGGACACGGGCAGATGGGCAAGGATATTTCTCAATTAACAACATTAGGCCTGGAAACTACAATCTATACGCGTGGGTACCTGGTGTCATCGGTGATTACAAGTACGCCAATGACATCACTGTCACGTTGGGGAGTACCACCCGCCTCGGCTTACTCATATACGACCCTCCAAGACAAGGCCCAACTCTGTGGGAGATTGGCGTCCCAGACCGGACAGCCGCCGAGTTCTATATACCGGATCCCCAGCCCACCCTCATGAATCGCTTTTACAACAATTCTGATATCCATACCCCAGATAAGTTTCGACAGTACGGTCTTTGGGAGCGCTACGCCGAATTGTTTCCCCATCAAGATCTAGTCTACAACGTCGCCACCGATGACTACCGCCGACATTGGTTCTTCGCCCATGTCACCAGAGCCACCCGTTTCGGTACCTACCAAGCAACCACGTGGCAGATTGTGTTTCGTCTTCAAGGGCCCAAACAGAGCGGGAACTATATGCTTCGGTTGGCCTTGGCTTCCACTACCGAGGCGGTGGTCGAAATCCGGTTCAACAACCCGTTGGCCAGAGGGGCTCATTTCTCGACCGGGTATTGGGCCTCAGGAAGGGATAACGCCATTGCTAGACATGGGATTCATGGGCTTTATTGGTTATTCAACTTCAAGGTTCCAAGTGGATATTTGGTCGAGGATGAAAACATTATCTATCTTACACAGAGACGACATATGGGACCCTTTCAAGGAGTGATGTACGATTACATACGGTTCGAAGGCGTACAATAA

Protein sequence

MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Homology
BLAST of CmoCh03G008910 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.6e-33
Identity = 158/629 (25.12%), Postives = 251/629 (39.90%), Query Frame = 0

Query: 79  MDNGLVRITLSRPEGNVIALKYNALDNLLE-----IRNPQYNRGYWDVVWNRPNRGSATD 138
           +DNGL+++     +G+   + +    NL+       R+P  NR ++   ++    G   +
Sbjct: 40  LDNGLLKVRFG-ADGSAKEV-WKGGTNLISRLSGAARDPDKNRSFYLDYYS----GGVNE 99

Query: 139 RVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLD 198
            V     +VI  T DQ+ L++    N        L ++   I+ R  SG Y+Y +     
Sbjct: 100 FV-PERLEVIKQTPDQVHLAYIDDQNGK------LRLEYHLIMTRDVSGLYSYVVAANTG 159

Query: 199 GWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINS 258
             P + V ++R V++             D  R+         G PL Y E   L +  + 
Sbjct: 160 SAP-VTVSELRNVYRF------------DATRLDTLFNSIRRGTPLLYDELEQLPKVQDE 219

Query: 259 KLK---GEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVG 318
             +   G V  KY ++    E++   W  M    G WM+  S E+ +   +KQ+L  H  
Sbjct: 220 TWRLPDGSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEYYSGDALKQELLVHQD 279

Query: 319 PTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRI 378
              L+    +H+   ++  + G    ++K++GP  +Y+N          L  D   +   
Sbjct: 280 AIILNYLTGSHFGTPDMVAQPG----FEKLYGPWLLYINQ----GNDRELVADVSRRAEH 339

Query: 379 ETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQT 438
           E + WPY ++    +P   QR TVSGRL     HA           V L +  E  ++  
Sbjct: 340 ERASWPYRWLDDARYP--RQRATVSGRLRTEAPHAT----------VVLNSSAE--NFDI 399

Query: 439 ESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLI 498
           ++ GY F  R +  G FS++N+ PG Y L A+  G       A       G  TRLG + 
Sbjct: 400 QTTGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQTVRVEGKKTRLGQI- 459

Query: 499 YDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAEL 558
            D  +  P  W IG  DR A EF           RF         DK RQY    R+   
Sbjct: 460 -DARQPAPLAWAIGQADRRADEF-----------RF--------GDKPRQY----RWQTE 519

Query: 559 FPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALA 618
            P  DL + +     R+ W++A           Q  +W I+F  + P+Q     + +A A
Sbjct: 520 VP-ADLTFEIGKSRERKDWYYAQT---------QPGSWHILFNTRTPEQPYTLNIAIAAA 571

Query: 619 S----TTEA---VVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIH-GLYWLFNFKVPS 678
           S    TT A    + ++ N  L     +         D +I R  +  G Y   +  +P+
Sbjct: 580 SNNGMTTPASSPQLAVKLNGQLLTTLKYD-------NDKSIYRGAMQSGRYHEAHIPLPA 571

Query: 679 GYLVEDENIIYLTQRRHMGPFQGVMYDYI 692
           G L +  N I L     M     VMYD I
Sbjct: 640 GALQQGGNRITLELLGGM-----VMYDAI 571

BLAST of CmoCh03G008910 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 7.3e-18
Identity = 106/470 (22.55%), Postives = 187/470 (39.79%), Query Frame = 0

Query: 72  ENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDV------VWNR 131
           EN   +V++N  +R T  +  G++I L  +  D L           Y D        +  
Sbjct: 24  ENDTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGIGPYLDCYCTPSGFYTA 83

Query: 132 PNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYA 191
            +    T+ V+ T+    +       +    T+ P           + + LR G++GF+ 
Sbjct: 84  GSTNPVTELVQGTD----STGTKYAGIILNDTYTPTGQ-----EFQQYWFLRDGETGFH- 143

Query: 192 YAIFERLDGWPQI-----DVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 251
             +F RL  + +      ++ ++R +F+     + ++  SD +   +P+  D      + 
Sbjct: 144 --MFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSDLQTAPLPS--DEAIAEQIV 203

Query: 252 YREAV--LLTQPINSKLK--GEVDDKYQYSLENMENKVHGW-----ESMNPHVGFWMITP 311
            ++A   L   P ++  +   E   KY +S    +N VHG       S     G W++  
Sbjct: 204 VQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMN 263

Query: 312 SDEFRTAGPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSD 371
           + +    GP+  DLT  V     +  VS H+     ++  G    + + FGP F   N  
Sbjct: 264 TKDTYYGGPLHSDLT--VDGIIYNYIVSNHHGEGTPNITNG----FDRTFGPQFYLFN-- 323

Query: 372 PVGSGPGSLWEDAKNQMRIETSKWPYSFVGS-----TDFPSSNQRGTVSGRLLVRDRHAK 431
             G G  SL E       + +  W   F  S       +  S+QRG+V G + +  ++AK
Sbjct: 324 --GGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTIKL-PKNAK 383

Query: 432 KWLMWGDSAYVGLAAPGELGSWQTES---KGYQFWTRADGQGYFSINNIRPGNYNLYAWV 491
                  S    L   G    +Q  S     +Q+W   D  G FSI+ +  G Y L  + 
Sbjct: 384 -------SPIAVLTVDGHY--FQDNSAVPSSHQYWADIDKNGRFSIDRVVAGKYRLTVYA 443

Query: 492 PGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEF 514
            G+ GD+   + I V    +T +    + P   G  +W +G PD+++ EF
Sbjct: 444 DGIFGDFT-RDGIVVKARKSTSI-KETWKPESAGTEIWRLGTPDKSSGEF 457

BLAST of CmoCh03G008910 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.1e-16
Identity = 75/318 (23.58%), Postives = 130/318 (40.88%), Query Frame = 0

Query: 262 KYQYSLENMENKVHGW-----ESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSM 321
           KY +S    +N VHG       S     G W++  + +    GP+  DLT  V     + 
Sbjct: 216 KYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNY 275

Query: 322 FVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSG-PGSLWEDAKNQMRIETSKW 381
            VS H+     ++ +G    + + FGP + + N    G G   SL E   +   +    W
Sbjct: 276 LVSNHHGEGTPNITYG----FDRTFGPQYYHFNG---GKGSTASLQELKSDAETLADPSW 335

Query: 382 PYSFVGS-----TDFPSSNQRGTVSGRL-LVRDRHAKKWLMWGDSAYVGLAAPGELGSWQ 441
              F  S       +  S+QRG+V G++ L +       ++  D  Y             
Sbjct: 336 NVDFYDSIAKHVVGYTPSSQRGSVQGKIKLPKGATRPIAVLTVDGQY--------FQDNS 395

Query: 442 TESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLL 501
             S  YQ+W   D  G+FS+++++ G Y L  +  G+ GD+   + + V  G  T +   
Sbjct: 396 VNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDF-VRDGVQVKAGKKTTI-QE 455

Query: 502 IYDPPRQGPTLWEIGVPDRTAAEF---YIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWER 561
            ++    G  +W +G PD+++ EF      DP             +H P+    +G ++ 
Sbjct: 456 TWEAESAGTEIWRLGTPDKSSGEFRHGVARDP----------THPLHPPEYLIYWGAYDW 502

Query: 562 YAELFPHQDLVYNVATDD 565
            ++ FP   + Y + T D
Sbjct: 516 QSD-FP-DGINYTIGTSD 502

BLAST of CmoCh03G008910 vs. ExPASy TrEMBL
Match: A0A6J1IPB6 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111478723 PE=3 SV=1)

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 668/697 (95.84%), Postives = 678/697 (97.27%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNE 60
           MEK+GKARRQ PYWTWKLGFSAMPLLFLFFSLTLAH NSSQ       RRMVRKLVKNNE
Sbjct: 40  MEKKGKARRQRPYWTWKLGFSAMPLLFLFFSLTLAHKNSSQ-------RRMVRKLVKNNE 99

Query: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120
           SMLSRDVT+EWENRGQVVMDNGLVRITLS PEGNV+ALKYNA+DNLLEIRNPQYNRGYWD
Sbjct: 100 SMLSRDVTVEWENRGQVVMDNGLVRITLSSPEGNVVALKYNAIDNLLEIRNPQYNRGYWD 159

Query: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180
           VVWNRPNRGSATDRVRAT+FKVITAT DQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD
Sbjct: 160 VVWNRPNRGSATDRVRATDFKVITATTDQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 219

Query: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240
           SGFYAYAIFERLDGWPQIDVDQIR+VFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA
Sbjct: 220 SGFYAYAIFERLDGWPQIDVDQIRMVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 279

Query: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300
           YREAVLLTQPINS LKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP
Sbjct: 280 YREAVLLTQPINSNLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 339

Query: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSL 360
           VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFG GEAWKKVFGPVFIYLNS PVG+GPG L
Sbjct: 340 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGEGEAWKKVFGPVFIYLNSGPVGTGPGLL 399

Query: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420
           WEDAKNQMRIETS+WPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA
Sbjct: 400 WEDAKNQMRIETSRWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 459

Query: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480
           APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAW+PGVIGDYKYANDITVTL
Sbjct: 460 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWIPGVIGDYKYANDITVTL 519

Query: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540
           GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDP PTLMNRFYNNSDIHTPDKFRQ
Sbjct: 520 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPHPTLMNRFYNNSDIHTPDKFRQ 579

Query: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600
           YGLWERYAELFP QDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS
Sbjct: 580 YGLWERYAELFPRQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 639

Query: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660
           GNYMLRLALASTTEAVVEIRFNNPLAR AHF TGYWASGRDNAIARHGIHGLYWLFNF+V
Sbjct: 640 GNYMLRLALASTTEAVVEIRFNNPLARRAHFLTGYWASGRDNAIARHGIHGLYWLFNFQV 699

Query: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Sbjct: 700 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 729

BLAST of CmoCh03G008910 vs. ExPASy TrEMBL
Match: A0A6J1GG74 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111453886 PE=3 SV=1)

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 638/697 (91.54%), Postives = 638/697 (91.54%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNE 60
           MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ       RRMVRKLVKNNE
Sbjct: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ-------RRMVRKLVKNNE 60

Query: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120
           SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD
Sbjct: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120

Query: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180
           VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD
Sbjct: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180

Query: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240
           SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA
Sbjct: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240

Query: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300
           YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP
Sbjct: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300

Query: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSL 360
           VKQDLTSHVGPTTLS                                             
Sbjct: 301 VKQDLTSHVGPTTLS--------------------------------------------- 360

Query: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420
                  MRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA
Sbjct: 361 -------MRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420

Query: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480
           APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL
Sbjct: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480

Query: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540
           GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ
Sbjct: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540

Query: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600
           YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS
Sbjct: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600

Query: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660
           GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV
Sbjct: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 638

Query: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Sbjct: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 638

BLAST of CmoCh03G008910 vs. ExPASy TrEMBL
Match: A0A1S3B1X3 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484918 PE=3 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 525/688 (76.31%), Postives = 585/688 (85.03%), Query Frame = 0

Query: 20  FSAM-PLLFLFFSLTLA-------HNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEW 79
           FS M  LLFLFF L LA         + SQ++LV N          NN++MLSRDVTLE 
Sbjct: 19  FSTMAALLFLFFGLALAMAVPVPQQQDFSQQILVEN----------NNKTMLSRDVTLEL 78

Query: 80  ENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSA 139
           +N GQV++DNG+VR+T SRP+GNVIALKY  +DNLLEI N  YNRGYWDVVWNRPN+ SA
Sbjct: 79  QNHGQVIIDNGIVRVTFSRPDGNVIALKYKGVDNLLEIHNVHYNRGYWDVVWNRPNKASA 138

Query: 140 TDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFER 199
           TDRVRATNFKVITA  DQ+ELSFTKTW+P+  SS PLNIDKRYI+RRGDSGFY+Y IFER
Sbjct: 139 TDRVRATNFKVITANDDQVELSFTKTWDPSDASSLPLNIDKRYIVRRGDSGFYSYGIFER 198

Query: 200 LDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPI 259
           L+GWPQIDVDQIRI +K+LG KFHYM VSDDRQR+MPTA DR  G PL Y EAVLLT+P 
Sbjct: 199 LNGWPQIDVDQIRIAYKLLGDKFHYMAVSDDRQRMMPTAEDRLAGKPLDYPEAVLLTRPA 258

Query: 260 NSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGP 319
           +   +GEVDDKYQYSLENMEN+VHGWES +PHVGFWMITPSDEFRTAGPVKQDLTSHVGP
Sbjct: 259 DPSFQGEVDDKYQYSLENMENRVHGWESSDPHVGFWMITPSDEFRTAGPVKQDLTSHVGP 318

Query: 320 TTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGS-GPGSLWEDAKNQMRI 379
            TLSMFVSTHYAGK+VSMRF  GEAWKKVFGPVF YLNSDP G+  PGSLWEDAKNQM I
Sbjct: 319 ITLSMFVSTHYAGKDVSMRFREGEAWKKVFGPVFTYLNSDPTGTDNPGSLWEDAKNQMTI 378

Query: 380 ETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQT 439
           ETS+WPY+FV S +FPSS+QRG++ G+LLVRDR+AK   MW DSAYVGLAAPGELGSWQ 
Sbjct: 379 ETSRWPYNFVESREFPSSDQRGSLRGQLLVRDRYAKYPQMWADSAYVGLAAPGELGSWQR 438

Query: 440 ESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLI 499
           ESKGYQFWTRAD  GYFSI+NIR G YNLYAWVPGV+GDYKY  +IT+T  S+T L LL+
Sbjct: 439 ESKGYQFWTRADEHGYFSIDNIRSGTYNLYAWVPGVLGDYKYDKEITITPRSSTNLNLLV 498

Query: 500 YDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHT-PDKFRQYGLWERYAE 559
           +DPPRQGPTLWEIG+PDR AAEFYIP P+PTLMNRFYN+S IH   D FRQYGLWERYAE
Sbjct: 499 FDPPRQGPTLWEIGIPDRKAAEFYIPSPRPTLMNRFYNDSKIHIHDDNFRQYGLWERYAE 558

Query: 560 LFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLAL 619
           L+PHQDLVYNV TDDYRR+WFFAHVTRAT  G Y+ATTWQIVFRLQ PK+ GNYMLRLAL
Sbjct: 559 LYPHQDLVYNVDTDDYRRNWFFAHVTRATNSGIYEATTWQIVFRLQRPKKIGNYMLRLAL 618

Query: 620 ASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDE 679
           AS+TEAV++IRFNNP  +  HF+TGYW+SG+DNAIARHGIHGLYWLFNFKVPS YLVE E
Sbjct: 619 ASSTEAVIDIRFNNPSTKRTHFTTGYWSSGKDNAIARHGIHGLYWLFNFKVPSQYLVEGE 678

Query: 680 NIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           N+IYLTQRRH G FQGVMYDYIRFE V+
Sbjct: 679 NVIYLTQRRHTGAFQGVMYDYIRFEDVE 696

BLAST of CmoCh03G008910 vs. ExPASy TrEMBL
Match: A0A0A0KIQ2 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_6G497340 PE=3 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 511/680 (75.15%), Postives = 579/680 (85.15%), Query Frame = 0

Query: 25  LLFLFFSLTLA-----HNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEWENRGQVVM 84
           LLF FF L LA       +SSQ++L  N          NN++MLSRDVTLE +N GQV++
Sbjct: 4   LLFFFFGLALAMAVPPQQDSSQQILEEN----------NNKAMLSRDVTLELQNHGQVII 63

Query: 85  DNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATN 144
           DNG+VR+TLSRP+G+VIALKYN +DNLLEIRN Q NRGYWDVVWNRPN+ SATDRV ATN
Sbjct: 64  DNGIVRVTLSRPDGDVIALKYNGIDNLLEIRNVQSNRGYWDVVWNRPNKASATDRVIATN 123

Query: 145 FKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPQID 204
           FKVIT+ +DQ+ELSF KTW+P+  SS P NIDKRYILRRGDSGFY+Y IFERL+GWPQI+
Sbjct: 124 FKVITSNSDQVELSFAKTWDPSDPSSLPSNIDKRYILRRGDSGFYSYGIFERLNGWPQIE 183

Query: 205 VDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINSKLKGEV 264
           VDQIRI +K+LG KFHYM +SD+RQR+MPTA DR TG PL Y EAVLLT+P +   +GEV
Sbjct: 184 VDQIRIAYKLLGDKFHYMAISDNRQRMMPTAEDRLTGKPLDYPEAVLLTRPRDPSFRGEV 243

Query: 265 DDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSMFVS 324
           DDKYQYSLENMENKVHGWES +PHVGFWMITPSDEFRTAGPVKQDLTSHVGP TLSMFVS
Sbjct: 244 DDKYQYSLENMENKVHGWESSDPHVGFWMITPSDEFRTAGPVKQDLTSHVGPVTLSMFVS 303

Query: 325 THYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSG-PGSLWEDAKNQMRIETSKWPYS 384
           THYAGK+VSMRF  GEAWKKVFGPVF Y+NSDP G+G PGSLW+DAKNQMRIETSKWPY+
Sbjct: 304 THYAGKDVSMRFQEGEAWKKVFGPVFTYINSDPTGTGKPGSLWDDAKNQMRIETSKWPYN 363

Query: 385 FVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFW 444
           FV ST+FPSS QRG++SG+LLVRD  AK   MWGDSAYVGLAAPGELGSWQ ESKGYQFW
Sbjct: 364 FVESTEFPSSEQRGSLSGQLLVRDGFAKYPQMWGDSAYVGLAAPGELGSWQRESKGYQFW 423

Query: 445 TRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGP 504
           TRAD  GYFSI+NIR G YNLYAWVPGV+GDYKY N IT+T  S+T L LL++ PPRQGP
Sbjct: 424 TRADDHGYFSIDNIRSGTYNLYAWVPGVLGDYKYDNQITITPRSSTNLNLLVFHPPRQGP 483

Query: 505 TLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIH-TPDKFRQYGLWERYAELFPHQDLV 564
           TLWEIG+PDR AAEFYIP P+PTL N FYN+S +H   D FRQYGLWERYAEL+PH DLV
Sbjct: 484 TLWEIGIPDRKAAEFYIPSPRPTLTNCFYNDSKLHIQDDNFRQYGLWERYAELYPHGDLV 543

Query: 565 YNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVV 624
           YNV TDDY R+WF+AHVTRAT  GTY+ATTWQIVF +Q PKQ GNYMLRLALAS+T+AV+
Sbjct: 544 YNVDTDDYHRNWFYAHVTRATGSGTYEATTWQIVFHIQRPKQIGNYMLRLALASSTDAVI 603

Query: 625 EIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQR 684
           +IRFNNP  +  HF+TGYW+SG+DNAIARHGIHGL+WLFNF+VPS  LVE +N+IYLTQR
Sbjct: 604 DIRFNNPSTKQPHFTTGYWSSGKDNAIARHGIHGLHWLFNFQVPSQLLVEGQNVIYLTQR 663

Query: 685 RHMGPFQGVMYDYIRFEGVQ 698
           RH G FQGVMYDYIRFE V+
Sbjct: 664 RHTGEFQGVMYDYIRFEDVE 673

BLAST of CmoCh03G008910 vs. ExPASy TrEMBL
Match: A0A1S3B219 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484918 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 2.2e-307
Identity = 494/623 (79.29%), Postives = 548/623 (87.96%), Query Frame = 0

Query: 77  VVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVR 136
           V++DNG+VR+T SRP+GNVIALKY  +DNLLEI N  YNRGYWDVVWNRPN+ SATDRVR
Sbjct: 2   VIIDNGIVRVTFSRPDGNVIALKYKGVDNLLEIHNVHYNRGYWDVVWNRPNKASATDRVR 61

Query: 137 ATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWP 196
           ATNFKVITA  DQ+ELSFTKTW+P+  SS PLNIDKRYI+RRGDSGFY+Y IFERL+GWP
Sbjct: 62  ATNFKVITANDDQVELSFTKTWDPSDASSLPLNIDKRYIVRRGDSGFYSYGIFERLNGWP 121

Query: 197 QIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINSKLK 256
           QIDVDQIRI +K+LG KFHYM VSDDRQR+MPTA DR  G PL Y EAVLLT+P +   +
Sbjct: 122 QIDVDQIRIAYKLLGDKFHYMAVSDDRQRMMPTAEDRLAGKPLDYPEAVLLTRPADPSFQ 181

Query: 257 GEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSM 316
           GEVDDKYQYSLENMEN+VHGWES +PHVGFWMITPSDEFRTAGPVKQDLTSHVGP TLSM
Sbjct: 182 GEVDDKYQYSLENMENRVHGWESSDPHVGFWMITPSDEFRTAGPVKQDLTSHVGPITLSM 241

Query: 317 FVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGS-GPGSLWEDAKNQMRIETSKW 376
           FVSTHYAGK+VSMRF  GEAWKKVFGPVF YLNSDP G+  PGSLWEDAKNQM IETS+W
Sbjct: 242 FVSTHYAGKDVSMRFREGEAWKKVFGPVFTYLNSDPTGTDNPGSLWEDAKNQMTIETSRW 301

Query: 377 PYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGY 436
           PY+FV S +FPSS+QRG++ G+LLVRDR+AK   MW DSAYVGLAAPGELGSWQ ESKGY
Sbjct: 302 PYNFVESREFPSSDQRGSLRGQLLVRDRYAKYPQMWADSAYVGLAAPGELGSWQRESKGY 361

Query: 437 QFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPR 496
           QFWTRAD  GYFSI+NIR G YNLYAWVPGV+GDYKY  +IT+T  S+T L LL++DPPR
Sbjct: 362 QFWTRADEHGYFSIDNIRSGTYNLYAWVPGVLGDYKYDKEITITPRSSTNLNLLVFDPPR 421

Query: 497 QGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHT-PDKFRQYGLWERYAELFPHQ 556
           QGPTLWEIG+PDR AAEFYIP P+PTLMNRFYN+S IH   D FRQYGLWERYAEL+PHQ
Sbjct: 422 QGPTLWEIGIPDRKAAEFYIPSPRPTLMNRFYNDSKIHIHDDNFRQYGLWERYAELYPHQ 481

Query: 557 DLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTE 616
           DLVYNV TDDYRR+WFFAHVTRAT  G Y+ATTWQIVFRLQ PK+ GNYMLRLALAS+TE
Sbjct: 482 DLVYNVDTDDYRRNWFFAHVTRATNSGIYEATTWQIVFRLQRPKKIGNYMLRLALASSTE 541

Query: 617 AVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYL 676
           AV++IRFNNP  +  HF+TGYW+SG+DNAIARHGIHGLYWLFNFKVPS YLVE EN+IYL
Sbjct: 542 AVIDIRFNNPSTKRTHFTTGYWSSGKDNAIARHGIHGLYWLFNFKVPSQYLVEGENVIYL 601

Query: 677 TQRRHMGPFQGVMYDYIRFEGVQ 698
           TQRRH G FQGVMYDYIRFE V+
Sbjct: 602 TQRRHTGAFQGVMYDYIRFEDVE 624

BLAST of CmoCh03G008910 vs. NCBI nr
Match: KAG7034239.1 (rhiE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1438.3 bits (3722), Expect = 0.0e+00
Identity = 684/697 (98.13%), Postives = 685/697 (98.28%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNE 60
           MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ       RRMVRKLVKNNE
Sbjct: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ-------RRMVRKLVKNNE 60

Query: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120
           SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD
Sbjct: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120

Query: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180
           VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD
Sbjct: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180

Query: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240
           SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA
Sbjct: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240

Query: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300
           YREAVLLTQP+NS LKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP
Sbjct: 241 YREAVLLTQPVNSNLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300

Query: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSL 360
           VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSG GSL
Sbjct: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGTGSL 360

Query: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420
           WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA
Sbjct: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420

Query: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480
           APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL
Sbjct: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480

Query: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540
           GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ
Sbjct: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540

Query: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600
           YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTR TRFGTYQATTWQIVFRLQGPKQS
Sbjct: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRGTRFGTYQATTWQIVFRLQGPKQS 600

Query: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660
           GNY LRLALASTTEAVVEIRFNNPLAR AHFSTGYWASGRDNAIARHGIHGLYWLFNFKV
Sbjct: 601 GNYTLRLALASTTEAVVEIRFNNPLARRAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660

Query: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Sbjct: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 690

BLAST of CmoCh03G008910 vs. NCBI nr
Match: XP_022978916.1 (uncharacterized protein LOC111478723 [Cucurbita maxima])

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 668/697 (95.84%), Postives = 678/697 (97.27%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNE 60
           MEK+GKARRQ PYWTWKLGFSAMPLLFLFFSLTLAH NSSQ       RRMVRKLVKNNE
Sbjct: 40  MEKKGKARRQRPYWTWKLGFSAMPLLFLFFSLTLAHKNSSQ-------RRMVRKLVKNNE 99

Query: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120
           SMLSRDVT+EWENRGQVVMDNGLVRITLS PEGNV+ALKYNA+DNLLEIRNPQYNRGYWD
Sbjct: 100 SMLSRDVTVEWENRGQVVMDNGLVRITLSSPEGNVVALKYNAIDNLLEIRNPQYNRGYWD 159

Query: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180
           VVWNRPNRGSATDRVRAT+FKVITAT DQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD
Sbjct: 160 VVWNRPNRGSATDRVRATDFKVITATTDQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 219

Query: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240
           SGFYAYAIFERLDGWPQIDVDQIR+VFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA
Sbjct: 220 SGFYAYAIFERLDGWPQIDVDQIRMVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 279

Query: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300
           YREAVLLTQPINS LKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP
Sbjct: 280 YREAVLLTQPINSNLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 339

Query: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSL 360
           VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFG GEAWKKVFGPVFIYLNS PVG+GPG L
Sbjct: 340 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGEGEAWKKVFGPVFIYLNSGPVGTGPGLL 399

Query: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420
           WEDAKNQMRIETS+WPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA
Sbjct: 400 WEDAKNQMRIETSRWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 459

Query: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480
           APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAW+PGVIGDYKYANDITVTL
Sbjct: 460 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWIPGVIGDYKYANDITVTL 519

Query: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540
           GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDP PTLMNRFYNNSDIHTPDKFRQ
Sbjct: 520 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPHPTLMNRFYNNSDIHTPDKFRQ 579

Query: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600
           YGLWERYAELFP QDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS
Sbjct: 580 YGLWERYAELFPRQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 639

Query: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660
           GNYMLRLALASTTEAVVEIRFNNPLAR AHF TGYWASGRDNAIARHGIHGLYWLFNF+V
Sbjct: 640 GNYMLRLALASTTEAVVEIRFNNPLARRAHFLTGYWASGRDNAIARHGIHGLYWLFNFQV 699

Query: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Sbjct: 700 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 729

BLAST of CmoCh03G008910 vs. NCBI nr
Match: XP_022950941.1 (uncharacterized protein LOC111453886 [Cucurbita moschata])

HSP 1 Score: 1315.4 bits (3403), Expect = 0.0e+00
Identity = 638/697 (91.54%), Postives = 638/697 (91.54%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNE 60
           MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ       RRMVRKLVKNNE
Sbjct: 1   MEKRGKARRQHPYWTWKLGFSAMPLLFLFFSLTLAHNNSSQ-------RRMVRKLVKNNE 60

Query: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120
           SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD
Sbjct: 61  SMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWD 120

Query: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180
           VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD
Sbjct: 121 VVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGD 180

Query: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240
           SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA
Sbjct: 181 SGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLA 240

Query: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300
           YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP
Sbjct: 241 YREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGP 300

Query: 301 VKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSL 360
           VKQDLTSHVGPTTLS                                             
Sbjct: 301 VKQDLTSHVGPTTLS--------------------------------------------- 360

Query: 361 WEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420
                  MRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA
Sbjct: 361 -------MRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLA 420

Query: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480
           APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL
Sbjct: 421 APGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTL 480

Query: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540
           GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ
Sbjct: 481 GSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQ 540

Query: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600
           YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS
Sbjct: 541 YGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQS 600

Query: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 660
           GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV
Sbjct: 601 GNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKV 638

Query: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ
Sbjct: 661 PSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 638

BLAST of CmoCh03G008910 vs. NCBI nr
Match: XP_023543064.1 (uncharacterized protein LOC111803045 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 604/675 (89.48%), Postives = 610/675 (90.37%), Query Frame = 0

Query: 23  MPLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEWENRGQVVMDNG 82
           MPLLFLFFSLTLAHNNSSQ        RMVRKL KNNESML RDVTLEWENRGQVVMDNG
Sbjct: 1   MPLLFLFFSLTLAHNNSSQ--------RMVRKLAKNNESMLWRDVTLEWENRGQVVMDNG 60

Query: 83  LVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATNFKV 142
           LVRITLS PEGNV+ALKYNA+DNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATNFKV
Sbjct: 61  LVRITLSSPEGNVVALKYNAIDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATNFKV 120

Query: 143 ITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPQIDVDQ 202
           ITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWP+I+VDQ
Sbjct: 121 ITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPRIEVDQ 180

Query: 203 IRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINSKLKGEVDDK 262
           IRIVFKILGKKFHYMVVSDDR+RIMPTARDRETGHPLAYREAVLLTQPINS LKGEVDDK
Sbjct: 181 IRIVFKILGKKFHYMVVSDDRRRIMPTARDRETGHPLAYREAVLLTQPINSNLKGEVDDK 240

Query: 263 YQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSMFVSTHY 322
           YQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLS       
Sbjct: 241 YQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLS------- 300

Query: 323 AGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRIETSKWPYSFVGS 382
                                                        MRIETSKWPYSFVGS
Sbjct: 301 ---------------------------------------------MRIETSKWPYSFVGS 360

Query: 383 TDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFWTRAD 442
           TDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFWTRAD
Sbjct: 361 TDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFWTRAD 420

Query: 443 GQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWE 502
           GQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWE
Sbjct: 421 GQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWE 480

Query: 503 IGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAELFPHQDLVYNVAT 562
           IGVPDRTAAEFYIPDPQPTLMNRFYNNSDIH+PDKFRQYGLWERYAELFPHQDLVYNVAT
Sbjct: 481 IGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHSPDKFRQYGLWERYAELFPHQDLVYNVAT 540

Query: 563 DDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVVEIRFN 622
           DDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVVEIRFN
Sbjct: 541 DDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVVEIRFN 600

Query: 623 NPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQRRHMGP 682
           NPLAR AHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQRRHMGP
Sbjct: 601 NPLARRAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQRRHMGP 615

Query: 683 FQGVMYDYIRFEGVQ 698
           FQGVMYDYIRFEGVQ
Sbjct: 661 FQGVMYDYIRFEGVQ 615

BLAST of CmoCh03G008910 vs. NCBI nr
Match: XP_038882453.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1157.1 bits (2992), Expect = 0.0e+00
Identity = 557/700 (79.57%), Postives = 606/700 (86.57%), Query Frame = 0

Query: 1   MEKRGKARRQHPYWTWKLGFSAM-PLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVK-N 60
           MEK+GK RRQ      KL  SAM  L+FLFFS TLA     Q+      +RM R LV+ N
Sbjct: 1   MEKKGKRRRQQK----KLELSAMAALIFLFFSFTLAKAKPQQQQSF--QQRMRRNLVENN 60

Query: 61  NESMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGY 120
           N+  LSRDVTLEW+N GQVV+DNGLVR+TLSRP+GNVI LKY  +DN+LEI N Q+NRGY
Sbjct: 61  NKPTLSRDVTLEWQNHGQVVIDNGLVRVTLSRPDGNVIGLKYKGIDNVLEIHNAQHNRGY 120

Query: 121 WDVVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRR 180
           WDVVWNRPNR SATDRVRAT FKVIT  +DQIELSFTKTW+P+  SS PLNIDKRYILRR
Sbjct: 121 WDVVWNRPNRASATDRVRATEFKVITVNSDQIELSFTKTWDPSDSSSLPLNIDKRYILRR 180

Query: 181 GDSGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHP 240
           GD GFY+Y IFERLDGWPQIDVDQIRIV+K+ G KFHYM +SD+RQRIMPT +DR  G P
Sbjct: 181 GDYGFYSYGIFERLDGWPQIDVDQIRIVYKLQGDKFHYMAISDNRQRIMPTLQDRLMGRP 240

Query: 241 LAYREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTA 300
           LAY EAVLLT+PINS LKGEVDDKYQYSLENMENKVHGWES  PHVGFWMIT SDEFRTA
Sbjct: 241 LAYPEAVLLTRPINSNLKGEVDDKYQYSLENMENKVHGWESSEPHVGFWMITSSDEFRTA 300

Query: 301 GPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGP- 360
           GPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFG GEAWKKVFGPVF YLNSDP G+G  
Sbjct: 301 GPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGEGEAWKKVFGPVFTYLNSDPTGTGSL 360

Query: 361 GSLWEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYV 420
           GSLWEDAKNQMRIE SKWPY+FV S DFPSS+QRGTVSGRLLVRDR+AK   MW DSAYV
Sbjct: 361 GSLWEDAKNQMRIERSKWPYNFVESGDFPSSDQRGTVSGRLLVRDRYAKNSHMWADSAYV 420

Query: 421 GLAAPGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDIT 480
           GLAAPGE GSWQ ESKGYQFWTRAD QGYFSI+NIRPGNYNLYAWVPGV+ DYKY  +I 
Sbjct: 421 GLAAPGESGSWQKESKGYQFWTRADEQGYFSIDNIRPGNYNLYAWVPGVVADYKYDKEII 480

Query: 481 VTLGSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDK 540
           VT  S+T L LL++ PPRQGPTLWEIGVPDRTAAEFYIP+PQPTLMNRFYNNS  HT D 
Sbjct: 481 VTPRSSTNLNLLVFHPPRQGPTLWEIGVPDRTAAEFYIPNPQPTLMNRFYNNSKFHTQDN 540

Query: 541 FRQYGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGP 600
           FRQYGLWE+Y+EL+P+QDLVYNV TD+YRRHWFFAHVTRAT    Y+ATTWQIVFRLQGP
Sbjct: 541 FRQYGLWEQYSELYPNQDLVYNVTTDNYRRHWFFAHVTRATTSDNYEATTWQIVFRLQGP 600

Query: 601 KQSGNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFN 660
           KQ+GNYMLRLALAS+T+AVV+IRFNNP A   HFSTGYW+SG+DNAIARHGIHGLYWLFN
Sbjct: 601 KQTGNYMLRLALASSTDAVVDIRFNNPSATRPHFSTGYWSSGKDNAIARHGIHGLYWLFN 660

Query: 661 FKVPSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEGVQ 698
           F+VPS YLVEDEN+IYLTQRRH GPFQGVMYDYIRFEGV+
Sbjct: 661 FQVPSQYLVEDENVIYLTQRRHTGPFQGVMYDYIRFEGVE 694

BLAST of CmoCh03G008910 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 762.3 bits (1967), Expect = 3.4e-220
Identity = 368/671 (54.84%), Postives = 457/671 (68.11%), Query Frame = 0

Query: 25  LLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEWENRGQVVMDNGLV 84
           LL L   L   H+  SQ  L          + K  +++ +  V L       VV+DNG+V
Sbjct: 19  LLQLLLLLQSVHSQRSQNKL---------NITKPGQNIPALTVQLRRVGHDTVVVDNGIV 78

Query: 85  RITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRATNFKVIT 144
           ++T S PEG +  +KY+ +DN+L+ +    +RGYWDVVW  P +   TD++  T F++IT
Sbjct: 79  QVTFSNPEGLITGIKYHGIDNVLDDKID--DRGYWDVVWYEPEKKQKTDKLEGTKFEIIT 138

Query: 145 ATADQIELSFTKTWNPNSHSS-CPLNIDKRYILRRGDSGFYAYAIFERLDGWPQIDVDQI 204
              +QIE+SFT+TW  +   S  PLN+DKRYI+R G SG Y Y I ERL+GWP +D+DQI
Sbjct: 139 QNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLEGWPDVDMDQI 198

Query: 205 RIVFKILGKKFHYMVVSDDRQRIMPTARDRETGHPLAYREAVLLTQPINSKLKGEVDDKY 264
           RIVFK+  KKF +M +SDDRQR MP+  DRE    LAY+EAVLLT P N   KGEVDDKY
Sbjct: 199 RIVFKLNPKKFDFMAISDDRQRSMPSMADRENSKSLAYKEAVLLTNPSNPMFKGEVDDKY 258

Query: 265 QYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSMFVSTHYA 324
            YS+E+ +N VHGW S +P VGFWMITPSDEFR  GP+KQDLTSH GP TLSMF STHYA
Sbjct: 259 MYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTSHAGPITLSMFTSTHYA 318

Query: 325 GKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRIETSKWPYSFVGST 384
           GKE+ M +  GE WKKVFGPV  YLNS         LW DAK QM  E   WPY F+ S 
Sbjct: 319 GKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEVKSWPYDFITSE 378

Query: 385 DFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESKGYQFWTRADG 444
           D+P  +QRGT+ G+ L++D +  +  ++G  A+VGLA  GE GSWQTESKGYQFWT+AD 
Sbjct: 379 DYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTESKGYQFWTKADR 438

Query: 445 QGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQGPTLWEI 504
           +G F I N+R GNY+LYAW  G IGDYKY  +IT+T GS   +G L+Y+PPR GPTLWEI
Sbjct: 439 RGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYEPPRNGPTLWEI 498

Query: 505 GVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAELFPHQDLVYNVATD 564
           GVPDRTA EFYIPDP PTLMN+ Y N      D+FRQYGLW+RYA+L+P  DLVY +   
Sbjct: 499 GVPDRTAGEFYIPDPYPTLMNKLYVNP---LQDRFRQYGLWDRYADLYPQNDLVYTIGVS 558

Query: 565 DYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTTEAVVEIRFNN 624
           DYR  WFFAHV R     TYQ TTWQI+F L+   + G Y LR+ALAS  ++ ++IR N+
Sbjct: 559 DYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADSELQIRIND 618

Query: 625 PLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLTQRRHMGPF 684
           P +  A F+TG+   G+DNAIARHGIHGLY L++  V    L   +N I+LTQ R   PF
Sbjct: 619 PKS-DAIFTTGF--IGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQTRSRTPF 672

Query: 685 QGVMYDYIRFE 695
           QG+MYDYIR E
Sbjct: 679 QGIMYDYIRLE 672

BLAST of CmoCh03G008910 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 736.1 bits (1899), Expect = 2.6e-212
Identity = 354/626 (56.55%), Postives = 445/626 (71.09%), Query Frame = 0

Query: 79  MDNGLVRITLSRPEGNVIALKYNALDNLLE-IRNPQYNRGYWDVVWNRPNRGS-----AT 138
           M+N  +++TLS PEG V  ++YN +DN+L    N +Y+RGYWDVVWN P + +       
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 139 DRVRATNFKVITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERL 198
           DR+ AT  +VIT   ++IELSFT+TWN +S ++ P+NIDKR+++ +  SGFY+YAIFERL
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTSSTTAVPVNIDKRFVMLQNSSGFYSYAIFERL 120

Query: 199 DGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDR--ETGHPLAYREAVLLTQP 258
            GWP +++D +R+VFK+  KKFHYM +SDDRQR MP   DR    G PLAY EAV L  P
Sbjct: 121 QGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLDP 180

Query: 259 INSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVG 318
           I  + KGEVDDKY+YS+E+ + KVHGW S N  VGFW ITPS+EFR+AGP+KQ L SHVG
Sbjct: 181 IEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVG 240

Query: 319 PTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRI 378
           PT L++F STHY G ++ M F  GEAWKKVFGPVFIYLNS P G  P  LW +AKNQ +I
Sbjct: 241 PTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKI 300

Query: 379 ETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQT 438
           E  KWPY+F  S DFP+S+QRG+VSGRLLVRDR      +  + +YVGLAAPG++GSWQ 
Sbjct: 301 EEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQR 360

Query: 439 ESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLI 498
           E KGYQFW++AD  G FSINN+R G YNLYA+ PG IGDY       ++ GS   LG L+
Sbjct: 361 ECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLV 420

Query: 499 YDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAEL 558
           Y+PPR G TLWEIGVPDR+AAEFYIPDP P+ +N+ Y N      DK+RQYGLWERY+EL
Sbjct: 421 YEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHS----DKYRQYGLWERYSEL 480

Query: 559 FPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQ--SGNYMLRLA 618
           +P +D+VYNV  DDY ++WFF  VTR    G Y+ TTWQI F+     +  +GN+ LR+A
Sbjct: 481 YPDEDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIA 540

Query: 619 LASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVED 678
           LA++  A +++R N+  A    F T     GRDN IARHGIHGLYWL++  VP+  L   
Sbjct: 541 LATSNVAELQVRVNDLSADPPLFRTE--QIGRDNTIARHGIHGLYWLYSVNVPAASLHVG 600

Query: 679 ENIIYLTQRRHMGPFQGVMYDYIRFE 695
            N IYLTQ     PFQG+MYDYIR E
Sbjct: 601 NNTIYLTQALATSPFQGLMYDYIRLE 620

BLAST of CmoCh03G008910 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 734.2 bits (1894), Expect = 9.9e-212
Identity = 349/642 (54.36%), Postives = 443/642 (69.00%), Query Frame = 0

Query: 57  KNNESMLSRDVTLEWENRGQVVMDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNR 116
           + +  M S  V L   +R  VVMDNG++++TLS+P G +  ++YN +DN+LE+RN + NR
Sbjct: 39  RKDHRMSSHGVHLHVHDR-YVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNR 98

Query: 117 GYWDVVWNRPNRGSATDRVRATNFKVITATADQIELSFTKTWNPNSHSS-CPLNIDKRYI 176
           GYWD+ WN P      D +    F+VI  T +Q+E+SF +TW+P+      PLNIDKR+I
Sbjct: 99  GYWDLHWNEPGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFI 158

Query: 177 LRRGDSGFYAYAIFERLDGWPQIDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDRET 236
           + RG SG Y+Y I+E L  WP  ++ + RI FK+   KFHYM V+DDR+RIMP   D   
Sbjct: 159 MLRGSSGVYSYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCK 218

Query: 237 G--HPLAYREAVLLTQPINSKLKGEVDDKYQYSLENMENKVHGWESMNPHVGFWMITPSD 296
           G    L Y+EA LLT P + +L+GEVDDKYQYS EN + +VHGW S +P VGFW ITPS+
Sbjct: 219 GRCQTLDYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSN 278

Query: 297 EFRTAGPVKQDLTSHVGPTTLSMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPV 356
           EFR+ GP+KQ+LTSHVGPTTL++F STHYAGK +  RF  GE WKKV+GPVFIYLNS   
Sbjct: 279 EFRSGGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTAN 338

Query: 357 GSGPGSLWEDAKNQMRIETSKWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGD 416
           G  P  LW+DAK +M  E  +WPYSFV S D+P S +RGT  GRLL+RDR     L+   
Sbjct: 339 GDDPLCLWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISAR 398

Query: 417 SAYVGLAAPGELGSWQTESKGYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYA 476
            AYVGLA PG+ GSWQ E KGYQFW  AD  GYFSI N+RPG YNLYAWVP  IGDY   
Sbjct: 399 GAYVGLAPPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNG 458

Query: 477 NDITVTLGSTTRLGLLIYDPPRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIH 536
             + VT G    +G ++Y+PPR GPTLWEIG+PDR A+EF+IPDP PTL+NR      +H
Sbjct: 459 TIVRVTSGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVL----VH 518

Query: 537 TPDKFRQYGLWERYAELFPHQDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFR 596
             D+FRQYGLW++Y +++P+ DLVY V   DYRR WFFAHV R  +   ++ TTWQI+F 
Sbjct: 519 HQDRFRQYGLWKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPR-KKGDVHEGTTWQIIFN 578

Query: 597 LQGPKQSGNYMLRLALASTTEAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLY 656
           L+   Q  NY LR+A+AS T A ++IR N+  A    F+TG    GRDN+IARHGIHG+Y
Sbjct: 579 LENIDQKANYKLRVAIASATLAELQIRINDAEAIRPLFTTG--LIGRDNSIARHGIHGVY 638

Query: 657 WLFNFKVPSGYLVEDENIIYLTQRRHMGPFQGVMYDYIRFEG 696
            L+   +P   LV+ +N I+L Q R  GPFQG+MYDYIR EG
Sbjct: 639 MLYAVNIPGNRLVQGDNTIFLKQPRCNGPFQGIMYDYIRLEG 672

BLAST of CmoCh03G008910 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 731.9 bits (1888), Expect = 4.9e-211
Identity = 347/621 (55.88%), Postives = 435/621 (70.05%), Query Frame = 0

Query: 79  MDNGLVRITLSRPEGNVIALKYNALDNLLEIRNPQYNRGYWDVVWNRPNRGSATDRVRAT 138
           MDNG+ R+TLS+P+G V  ++YN +DNLLE+ N + NRGYWD+VW         D ++ +
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGSGTAGGFDVIKGS 60

Query: 139 NFKVITATADQIELSFTKTWNPNSH-SSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPQ 198
           NF+VI    +QIELSFT+ W+P+    + PLNIDKR+++  G SGFY YAI+E L  WP 
Sbjct: 61  NFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWPA 120

Query: 199 IDVDQIRIVFKILGKKFHYMVVSDDRQRIMPTARDR--ETGHPLAYREAVLLTQPINSKL 258
             + + RI FK+  +KFHYM V+DDRQR MP   DR  + G  LAY EAVLL  P+ S+ 
Sbjct: 121 FSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQF 180

Query: 259 KGEVDDKYQYSLENMENKVHGWE-SMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTL 318
           KGEVDDKYQYS EN +  VHGW  +  P VGFW+ITPS E+RT GP KQ+LTSHVGPT L
Sbjct: 181 KGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTAL 240

Query: 319 SMFVSTHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSD-PVGSGPGSLWEDAKNQMRIETS 378
           ++F+S HY G+++  +F  GEAWKKVFGPVF+YLNS     + P  LW+DAK+QM +E  
Sbjct: 241 AVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAE 300

Query: 379 KWPYSFVGSTDFPSSNQRGTVSGRLLVRDRHAKKWLMWGDSAYVGLAAPGELGSWQTESK 438
            WPYSF  S D+  + QRG V GRLLV+DR+  K  +  +  YVGLA PG  GSWQ E K
Sbjct: 301 SWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECK 360

Query: 439 GYQFWTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDP 498
            YQFWTR D +G+F I+ IRPG YNLYAW+PG IGDYKY + IT+T G    +  L+Y P
Sbjct: 361 EYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQP 420

Query: 499 PRQGPTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAELFPH 558
           PR G TLWEIG PDR+AAEFY+PDP P  +N  Y N     PD+FRQYGLWERYAEL+P 
Sbjct: 421 PRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNH----PDRFRQYGLWERYAELYPD 480

Query: 559 QDLVYNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLALASTT 618
           +DLVY V + DYR+ WF+A VTR     TYQ TTWQI F L+   ++ +Y LR+A+AS T
Sbjct: 481 KDLVYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASAT 540

Query: 619 EAVVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIY 678
            + ++IR NN  A    F++G    GRDN+IARHGIHGLYWLFN +V    L+E EN ++
Sbjct: 541 FSELQIRVNNANA-SPMFTSG--LIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLF 600

Query: 679 LTQRRHMGPFQGVMYDYIRFE 695
           LTQ R   PFQG+MYDYIRFE
Sbjct: 601 LTQPRSTSPFQGIMYDYIRFE 614

BLAST of CmoCh03G008910 vs. TAIR 10
Match: AT4G37950.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 723.0 bits (1865), Expect = 2.3e-208
Identity = 366/680 (53.82%), Postives = 453/680 (66.62%), Query Frame = 0

Query: 24  PLLFLFFSLTLAHNNSSQRLLVINCRRMVRKLVKNNESMLSRDVTLEWENRGQVVMDNGL 83
           PL  L F L   H+ +S+             + K    + S  VTL+     QVV+DNG+
Sbjct: 17  PLFVLVFLLQGVHSQTSRNKF---------NIAKAGHKLASPPVTLKKVGDNQVVVDNGI 76

Query: 84  VRITLSRPEGNVIALKYNALDNLLEIRNPQY-NRGYWDVVWNRPNRGSATDRVRATNFKV 143
           + +T S P+G +  +KYN L+N+L   N Q  NRGYWDVVW +P + S+TD +  T F +
Sbjct: 77  IDVTFSSPQGLITRIKYNGLNNVL---NDQIENRGYWDVVWYKPGQVSSTDYLVGTKFDI 136

Query: 144 ITATADQIELSFTKTWNPNSHSSCPLNIDKRYILRRGDSGFYAYAIFERLDGWPQIDVDQ 203
           +  T++QIE+SF++T++    S  PLN+DKRYI+RRG SG Y YA+ ERL GWP +D+DQ
Sbjct: 137 VNQTSEQIEISFSRTFSQRG-SGIPLNVDKRYIIRRGVSGIYMYAVLERLIGWPDVDMDQ 196

Query: 204 IRIVFKILGKKFHYMVVSDDRQRIMPTARDRE----TGHPLAYREAVLLTQPINSKLKGE 263
           IRIVFK+   KF +M VSD+RQ+IMP   DR+       PLAY+EAV L  P N  LKG+
Sbjct: 197 IRIVFKLNTTKFDFMAVSDNRQKIMPFDTDRDITKGRASPLAYKEAVHLINPQNHMLKGQ 256

Query: 264 VDDKYQYSLENMENKVHGWESMNPHVGFWMITPSDEFRTAGPVKQDLTSHVGPTTLSMFV 323
           VDDKY YS+EN +NKVHGW S +  +GFWMITPSDEF   GP+KQDLTSHVGPTTLSMF 
Sbjct: 257 VDDKYMYSVENKDNKVHGWISSDQRIGFWMITPSDEFHACGPIKQDLTSHVGPTTLSMFT 316

Query: 324 STHYAGKEVSMRFGVGEAWKKVFGPVFIYLNSDPVGSGPGSLWEDAKNQMRIETSKWPYS 383
           S HYAGK+++  +   E WKKVFGPVF+YLNS    S    LW DAK QM  E   WPY 
Sbjct: 317 SVHYAGKDMNTNYKSKEPWKKVFGPVFVYLNS---ASSRNLLWTDAKRQMVSEVQSWPYD 376

Query: 384 FVGSTDFPSSNQRGTVSGRLLVRDRHAKK-WLMWGDSAYVGLAAPGELGSWQTESKGYQF 443
           FV S D+P  +QRGTV G+L V DR+ K    ++G  A+VGLA PGE GSWQTE+KGYQF
Sbjct: 377 FVKSVDYPLHHQRGTVKGQLFVIDRYIKNVTYLFGQFAFVGLALPGEAGSWQTENKGYQF 436

Query: 444 WTRADGQGYFSINNIRPGNYNLYAWVPGVIGDYKYANDITVTLGSTTRLGLLIYDPPRQG 503
           WTRAD  G F+I N+RPG Y+LYAWV G IGDYKY  DIT+T G    +G ++Y PPR G
Sbjct: 437 WTRADKMGMFTIANVRPGTYSLYAWVSGFIGDYKYVRDITITPGREIDVGHIVYVPPRNG 496

Query: 504 PTLWEIGVPDRTAAEFYIPDPQPTLMNRFYNNSDIHTPDKFRQYGLWERYAELFPHQDLV 563
           PTLWEIG PDRTAAEFYIPDP PTL  + Y N   +  D+FRQYGLW+RY+ L+P  DLV
Sbjct: 497 PTLWEIGQPDRTAAEFYIPDPDPTLFTKLYLNYS-NPQDRFRQYGLWDRYSVLYPRNDLV 556

Query: 564 YNVATDDYRRHWFFAHVTRATRFGTYQATTWQIVFRLQGPKQSGNYMLRLAL--ASTTEA 623
           +     DY++ WF+AHV R    GTY+ATTWQI F L+   Q+  Y LR+AL  AST + 
Sbjct: 557 FTAGVSDYKKDWFYAHVNRKAGNGTYKATTWQIKFNLKAVIQTRIYTLRIALAAASTIDL 616

Query: 624 VVEIRFNNPLARGAHFSTGYWASGRDNAIARHGIHGLYWLFNFKVPSGYLVEDENIIYLT 683
           +V +   N +     F TG    GRDNAIARHGIHGLY L+N  V    L    N I+LT
Sbjct: 617 LVWV---NEVDSKPLFITG--LIGRDNAIARHGIHGLYKLYNIDVHGKLLRVGNNTIFLT 674

Query: 684 QRRHMGPFQGVMYDYIRFEG 696
             R+   F GVMYDY+R EG
Sbjct: 677 HGRNSDSFSGVMYDYLRLEG 674

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RJP21.6e-3325.12Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE... [more]
Q5AZ857.3e-1822.55Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ... [more]
A5ABH41.1e-1623.58Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F... [more]
Match NameE-valueIdentityDescription
A0A6J1IPB60.0e+0095.84Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111478723 PE=3 SV... [more]
A0A6J1GG740.0e+0091.54Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111453886 PE=3 ... [more]
A0A1S3B1X30.0e+0076.31Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484918 PE=3 SV=1[more]
A0A0A0KIQ20.0e+0075.15Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_6G497340 PE=3 SV=... [more]
A0A1S3B2192.2e-30779.29Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484918 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG7034239.10.0e+0098.13rhiE, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022978916.10.0e+0095.84uncharacterized protein LOC111478723 [Cucurbita maxima][more]
XP_022950941.10.0e+0091.54uncharacterized protein LOC111453886 [Cucurbita moschata][more]
XP_023543064.10.0e+0089.48uncharacterized protein LOC111803045 [Cucurbita pepo subsp. pepo][more]
XP_038882453.10.0e+0079.57probable rhamnogalacturonate lyase B [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G22620.13.4e-22054.84Rhamnogalacturonate lyase family protein [more]
AT1G09880.12.6e-21256.55Rhamnogalacturonate lyase family protein [more]
AT1G09910.19.9e-21254.36Rhamnogalacturonate lyase family protein [more]
AT1G09890.14.9e-21155.88Rhamnogalacturonate lyase family protein [more]
AT4G37950.12.3e-20853.82Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 499..692
e-value: 4.1E-47
score: 160.2
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 413..486
e-value: 3.5E-26
score: 90.8
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 390..487
e-value: 6.76512E-36
score: 128.135
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 70..351
e-value: 2.8E-14
score: 55.2
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 56..259
e-value: 2.3E-77
score: 259.2
NoneNo IPR availableGENE3D2.60.40.1120coord: 387..488
e-value: 2.2E-10
score: 42.6
NoneNo IPR availableGENE3D2.60.120.260coord: 497..693
e-value: 2.4E-10
score: 42.2
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 72..695
NoneNo IPR availablePANTHERPTHR32018:SF6RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 72..695
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 76..351
e-value: 3.17783E-70
score: 228.042
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 501..694
e-value: 8.07333E-41
score: 144.726
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 388..488
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 76..354
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 497..694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G008910.1CmoCh03G008910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0102210 rhamnogalacturonan endolyase activity
molecular_function GO:0003824 catalytic activity