CmoCh03G008320 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G008320
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	protein NEN4-like
Location	Cmo_Chr03: 6644887 .. 6647283 (+)
RNA-Seq Expression	CmoCh03G008320
Synteny	CmoCh03G008320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCTGAGATCGTCTTCTTCGACTTGGAAACTACAGTGCCCAATAGAATCGGGCAACGGTTTCGAGTATTGGAATTCGGTGCTATTGTCATCTGCCCCATGAAGCTTGTGGAGCTTCAGAGCTTTGCTACCCTCATCAGGCCGAAGGATCTGTCGGCTGTTTCATTGAGGTCCACCCGACCTGATGGGATAACAAGAGAAATCGTTGCCACTGCGCCATTGTTTGAGGAAGTTGCAGACAAGATTTTCAGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCGAAGATTCGATTGTGTTCGTATTGTTGGATGATGGAAGTCTCACATCGACTAATTTAGGGTGTGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATGGTATCAGAGCCATGCCCTAAACTTAGTCGTGCCAATAGATTGGTAAATCCTCAAATATCGAACAAAGAACTCTTAAAGAAAAGGAGCTAAGCCCCTCCTCGAAGGCAGTCAAAAATGACTAAGACTCCAAAGGAGTCGAGCCTCGATTAAGGGGAGGCGCACTTTGTTCGAGGGGAGGTGTTGGATGATGGAAGTCCCACATCGACTAATTTAGGGTGTGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATGGTATCAGAGCCATGCCCTAAACTTAGTCGTGCCAATAGATGGGTAAATCCTCGAATCCTCAAATATCGAACAAAGAACTCTTAAAGAAAAAGGAGCCAAGCCCCTCCTTGAAGGCAGTCAAAAATGACTAAGACTCCAAAGGAGTCGAGCCTCGATTAAGGGGAGGCGCACTTTGTTCGAGGGGAGGTGTTGGATGATGGAAGTCCCACATCGACTAATTTAGGGTGTGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATGGTATCAGAGCCATGCCCTAAACTTAGTCGTGCCAATAGATGGGTAAATCCTCGAATCCTCAAATATCGAACAAAGAACTCTTAAAGAAAAAGGAGCCAAGCCCCTCCTTGAAGGCAGTCAAAAATGACTAAGACTCCAAAGGAGTCGAGCCTCGATTAAGGGGAGGCGCACTTTGTTCGAGGGGAGGTGTTGGATGATGGAAGTCCCACATCGACTAATTTAGGGTGTGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACACGTATTAGGGAGGCGTTCGCGGAGATTGGCAGGCCTGCTCCTACCCCAGTTGGGATGGTTGATTCCTTAGGAATCTTAACTCACAAGTTTGGCAAGAGAGCAGGGAACATGAAGGTAAACCGTTAAAATCATAACATACAGCAATGTGTAATCACTACATTCAGAATAGTTCTCATGTTTTGAAGTGTTTCATGTTTAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGTATACATACAATCGGTTAACTCCCTCCCTTTGTGTGCGTGTGTCATGCCACCTTGGTTCACATAAGTTCTTTGTGCAGGAGCCTTGATGATGTTCGTATGAACTTGGAGGTCCTCAAGCATTGTGCTACTGTTCTATTTCTGGTGAGTCATCTCATCTAAACAGAATTCCTCTTCGTGGGTCATCTCTGATATTGGTAATTTTTGTACCAGGAATCGACCCTCCCAAGCATATTGCAGGGGAAGTGGAAGACTTCATCCACCATAACAACCCGAAACAGAGCAAATGGGAAGCTGCATTGCAGAGAGGAAAGTAGTCGAAAATCCCCTCCATCGTCGCCTGGGTATCAACTGAGATCAGCTCCTTATGCAAGGGAAAGACTGGGAATTGGAAAGGTTTAGTTACAGGTCTTTCTTTATACGCATAAAGGAAACATTTTTTCCCAACCAACTGATGAAAATGTGGTGAATTGAAACAGATGGCAGCTGAGGTAAAGAATGTATTCTGCATGGCTCACCGAAAGCCGCATCTTAACGTCTTACTTAAACATTCTCGATCGCTACTAAGGTAA

mRNA sequence

ATGGAAGCTGAGATCGTCTTCTTCGACTTGGAAACTACAGTGCCCAATAGAATCGGGCAACGGTTTCGAGTATTGGAATTCGGTGCTATTGTCATCTGCCCCATGAAGCTTGTGGAGCTTCAGAGCTTTGCTACCCTCATCAGGCCGAAGGATCTGTCGGCTGTTTCATTGAGGTCCACCCGACCTGATGGGATAACAAGAGAAATCGTTGCCACTGCGCCATTGTTTGAGGAAATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTTGATGATGTTCGTATGAACTTGGAGGTCCTCAAGCATTGTGCTACTGTTCTATTTCTGGAATCGACCCTCCCAAGCATATTGCAGGGGAAGTGGAAGACTTCATCCACCATAACAACCCGAAACAGAGCAAATGGGAAGCTGCATTGCAGAGAGGAAAGTAGTCGAAAATCCCCTCCATCGTCGCCTGGGTATCAACTGAGATCAGCTCCTTATGCAAGGGAAAGACTGGGAATTGGAAAGATGGCAGCTGAGGTAAAGAATGTATTCTGCATGGCTCACCGAAAGCCGCATCTTAACGTCTTACTTAAACATTCTCGATCGCTACTAAGGTAA

Coding sequence (CDS)

ATGGAAGCTGAGATCGTCTTCTTCGACTTGGAAACTACAGTGCCCAATAGAATCGGGCAACGGTTTCGAGTATTGGAATTCGGTGCTATTGTCATCTGCCCCATGAAGCTTGTGGAGCTTCAGAGCTTTGCTACCCTCATCAGGCCGAAGGATCTGTCGGCTGTTTCATTGAGGTCCACCCGACCTGATGGGATAACAAGAGAAATCGTTGCCACTGCGCCATTGTTTGAGGAAATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTTGATGATGTTCGTATGAACTTGGAGGTCCTCAAGCATTGTGCTACTGTTCTATTTCTGGAATCGACCCTCCCAAGCATATTGCAGGGGAAGTGGAAGACTTCATCCACCATAACAACCCGAAACAGAGCAAATGGGAAGCTGCATTGCAGAGAGGAAAGTAGTCGAAAATCCCCTCCATCGTCGCCTGGGTATCAACTGAGATCAGCTCCTTATGCAAGGGAAAGACTGGGAATTGGAAAGATGGCAGCTGAGGTAAAGAATGTATTCTGCATGGCTCACCGAAAGCCGCATCTTAACGTCTTACTTAAACATTCTCGATCGCTACTAAGGTAA

Protein sequence

MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPDGITREIVATAPLFEEMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKMAAEVKNVFCMAHRKPHLNVLLKHSRSLLR

Homology

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BLAST of CmoCh03G008320 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 5.8e-48
Identity = 123/268 (45.90%), Postives = 142/268 (52.99%), Query Frame = 0


Query: 4   EIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPD 63
           EIVFFDLET VPN+ GQ F +LEFGAI++CP KL EL+SF TLI+PKDLS VS+RS+R D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 64  GITREIVATAPLFEE--------------------------------------------- 123
           GITR  V  AP FE+                                             
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 124 ------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 183
                             MASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 184 SILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQL--RSAPYARERLGIGKMA 207
           + L+GKW TSS I TR+R N                   YQ+  R+ PY++  L   KM 
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

[Close]

BLAST of CmoCh03G008320 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 89/224 (39.73%), Postives = 111/224 (49.55%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFDLET VP + G+ F +LEFGAI++CP +L EL S++TL+RP DLS +S  + R 
Sbjct: 11  SEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLTKRR 70

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
            GITR+ V +A  F E                                            
Sbjct: 71  SGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKAT 130

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 159
                              MASLA+YF LG Q HRSLDDVRMNLEV+K+CATVLFLES++
Sbjct: 131 IDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLESSV 190

[Close]

BLAST of CmoCh03G008320 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 7.2e-30
Identity = 83/199 (41.71%), Postives = 103/199 (51.76%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFD+ETTVP R GQRF +LEFG+I++CP KL EL+S+ TL++P DLS +S  S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
           +GI R+ V  APLF +                                            
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 139
                              MA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

[Close]

BLAST of CmoCh03G008320 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-28
Identity = 82/219 (37.44%), Postives = 113/219 (51.60%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFD+ETT+P R+GQ + +LEFG+I++CP KLVEL++++ L+RP +L+ ++ RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
           +GI RE V +A  F +                                            
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 159
                              MA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

[Close]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL
Match: A0A6J1GEM9 (protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111453460 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.1e-100
Identity = 206/269 (76.58%), Postives = 206/269 (76.58%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTHKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL
Match: A0A6J1ILC9 (protein NEN4-like OS=Cucurbita maxima OX=3661 GN=LOC111478492 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.7e-98
Identity = 202/269 (75.09%), Postives = 204/269 (75.84%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIV+CPMKLVELQSFATLIRPKDLSAV+LRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVVCPMKLVELQSFATLIRPKDLSAVALRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTRKFGQRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL
Match: A0A5D3CL58 (Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002650 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.6e-86
Identity = 181/266 (68.05%), Postives = 190/266 (71.43%), Query Frame = 0


Query: 4   EIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPD 63
           EIVFFDLETTVP R+GQRFRVLEFGAIV+CPMKLVEL+SFATLI+P DLSAV+LRS+RPD
Sbjct: 17  EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTDLSAVALRSSRPD 76

Query: 64  GITREIVATAPLFEE--------------------------------------------- 123
           GITRE VATAPLFE+                                             
Sbjct: 77  GITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPTPVGMV 136

Query: 124 ------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 183
                             MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 137 DSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196

Query: 184 SILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKMAAE 207
           SILQ KWKTS T TTRNRANGKLHCREESSRKSPPSSPGYQLRS PY+RERLGIGKM A+
Sbjct: 197 SILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYSRERLGIGKMTAK 256

[Close]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL
Match: A0A1S3B2A8 (protein NEN4 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.6e-86
Identity = 181/266 (68.05%), Postives = 190/266 (71.43%), Query Frame = 0


Query: 4   EIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPD 63
           EIVFFDLETTVP R+GQRFRVLEFGAIV+CPMKLVEL+SFATLI+P DLSAV+LRS+RPD
Sbjct: 17  EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTDLSAVALRSSRPD 76

Query: 64  GITREIVATAPLFEE--------------------------------------------- 123
           GITRE VATAPLFE+                                             
Sbjct: 77  GITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPTPVGMV 136

Query: 124 ------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 183
                             MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 137 DSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196

Query: 184 SILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKMAAE 207
           SILQ KWKTS T TTRNRANGKLHCREESSRKSPPSSPGYQLRS PY+RERLGIGKM A+
Sbjct: 197 SILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYSRERLGIGKMTAK 256

[Close]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL
Match: A0A1S4DTI3 (protein NEN4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 3.1e-84
Identity = 181/270 (67.04%), Postives = 190/270 (70.37%), Query Frame = 0


Query: 4   EIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPD 63
           EIVFFDLETTVP R+GQRFRVLEFGAIV+CPMKLVEL+SFATLI+P DLSAV+LRS+RPD
Sbjct: 17  EIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTDLSAVALRSSRPD 76

Query: 64  GITREIVATAPLFEE--------------------------------------------- 123
           GITRE VATAPLFE+                                             
Sbjct: 77  GITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAEIGRPAPTPVGMV 136

Query: 124 ------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFL----E 183
                             MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFL    E
Sbjct: 137 DSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLFLHQE 196

Query: 184 STLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGK 207
           STLPSILQ KWKTS T TTRNRANGKLHCREESSRKSPPSSPGYQLRS PY+RERLGIGK
Sbjct: 197 STLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYSRERLGIGK 256

[Close]

BLAST of CmoCh03G008320 vs. NCBI nr
Match: XP_022950348.1 (protein NEN4-like [Cucurbita moschata])

HSP 1 Score: 374.4 bits (960), Expect = 6.3e-100
Identity = 206/269 (76.58%), Postives = 206/269 (76.58%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTHKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. NCBI nr
Match: KAG6604020.1 (Protein NEN4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 368.6 bits (945), Expect = 3.5e-98
Identity = 202/269 (75.09%), Postives = 204/269 (75.84%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIV+CPMKLVELQSFATLIRPKDLSAV+LRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVVCPMKLVELQSFATLIRPKDLSAVALRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTHKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNR NGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRENGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. NCBI nr
Match: XP_022978542.1 (protein NEN4-like [Cucurbita maxima])

HSP 1 Score: 367.5 bits (942), Expect = 7.7e-98
Identity = 202/269 (75.09%), Postives = 204/269 (75.84%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIV+CPMKLVELQSFATLIRPKDLSAV+LRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVVCPMKLVELQSFATLIRPKDLSAVALRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTRKFGQRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. NCBI nr
Match: KAG7034184.1 (Protein NEN4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 365.5 bits (937), Expect = 2.9e-97
Identity = 201/269 (74.72%), Postives = 203/269 (75.46%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIV+CPMKLVELQSFATLIRPKDLSAV+LRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVVCPMKLVELQSFATLIRPKDLSAVALRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILTHKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWKTSSTITTRNR NGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKTSSTITTRNRENGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. NCBI nr
Match: XP_023544691.1 (protein NEN4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.5 bits (924), Expect = 9.4e-96
Identity = 199/269 (73.98%), Postives = 201/269 (74.72%), Query Frame = 0


Query: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRST 60
           MEAEIVFFDLETTVPNRIGQRFRVLEFGAIV+CPMKLVELQSFATLIRPKDLSAV+LRST
Sbjct: 1   MEAEIVFFDLETTVPNRIGQRFRVLEFGAIVVCPMKLVELQSFATLIRPKDLSAVALRST 60

Query: 61  RPDGITREIVATAPLFEE------------------------------------------ 120
           RPDGITREIVATAPLFEE                                          
Sbjct: 61  RPDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIRRFDCVRIREAFAEIGRPAPTPV 120

Query: 121 ---------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180
                                MASLASYF LGQQKHRSLDDVRMNLEVLKHCATVLFLES
Sbjct: 121 GMVDSLGILSDKFGKRAGNMKMASLASYFHLGQQKHRSLDDVRMNLEVLKHCATVLFLES 180

Query: 181 TLPSILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 207
           TLPSILQGKWK SSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM
Sbjct: 181 TLPSILQGKWKASSTITTRNRANGKLHCREESSRKSPPSSPGYQLRSAPYARERLGIGKM 240

[Close]

BLAST of CmoCh03G008320 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 4.1e-49
Identity = 123/268 (45.90%), Postives = 142/268 (52.99%), Query Frame = 0


Query: 4   EIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRPD 63
           EIVFFDLET VPN+ GQ F +LEFGAI++CP KL EL+SF TLI+PKDLS VS+RS+R D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 64  GITREIVATAPLFEE--------------------------------------------- 123
           GITR  V  AP FE+                                             
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 124 ------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 183
                             MASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 184 SILQGKWKTSSTITTRNRANGKLHCREESSRKSPPSSPGYQL--RSAPYARERLGIGKMA 207
           + L+GKW TSS I TR+R N                   YQ+  R+ PY++  L   KM 
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

[Close]

BLAST of CmoCh03G008320 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.8e-32
Identity = 89/224 (39.73%), Postives = 111/224 (49.55%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFDLET VP + G+ F +LEFGAI++CP +L EL S++TL+RP DLS +S  + R 
Sbjct: 11  SEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLTKRR 70

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
            GITR+ V +A  F E                                            
Sbjct: 71  SGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKAT 130

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 159
                              MASLA+YF LG Q HRSLDDVRMNLEV+K+CATVLFLES++
Sbjct: 131 IDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLESSV 190

[Close]

BLAST of CmoCh03G008320 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 5.1e-31
Identity = 83/199 (41.71%), Postives = 103/199 (51.76%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFD+ETTVP R GQRF +LEFG+I++CP KL EL+S+ TL++P DLS +S  S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
           +GI R+ V  APLF +                                            
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 139
                              MA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

[Close]

BLAST of CmoCh03G008320 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 82/219 (37.44%), Postives = 113/219 (51.60%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFD+ETT+P R+GQ + +LEFG+I++CP KLVEL++++ L+RP +L+ ++ RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
           +GI RE V +A  F +                                            
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 159
                              MA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

[Close]

BLAST of CmoCh03G008320 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.9e-24
Identity = 80/224 (35.71%), Postives = 100/224 (44.64%), Query Frame = 0


Query: 3   AEIVFFDLETTVPNRIGQRFRVLEFGAIVICPMKLVELQSFATLIRPKDLSAVSLRSTRP 62
           +EI FFDLET VP + G+ F +LEFGAI++CP +L EL S++TL+RP DLS +S  + R 
Sbjct: 11  SEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLTKRR 70

Query: 63  DGITREIVATAPLFEE-------------------------------------------- 122
            GITR+ V +A  F E                                            
Sbjct: 71  SGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEPKAT 130

Query: 123 -------------------MASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 159
                              MASLA+YF LG Q HRSLDDVRMNLE           ES++
Sbjct: 131 IDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE-----------ESSV 190

The following BLAST results are available for this feature:

BLAST of CmoCh03G008320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJ23	5.8e-48	45.90	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]
Q9CA74	1.1e-30	39.73	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q9FLR0	7.2e-30	41.71	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	1.8e-28	37.44	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]

BLAST of CmoCh03G008320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEM9	3.1e-100	76.58	protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111453460 PE=4 SV=1	[more]
A0A6J1ILC9	3.7e-98	75.09	protein NEN4-like OS=Cucurbita maxima OX=3661 GN=LOC111478492 PE=4 SV=1	[more]
A0A5D3CL58	5.6e-86	68.05	Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3B2A8	5.6e-86	68.05	protein NEN4 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1	[more]
A0A1S4DTI3	3.1e-84	67.04	protein NEN4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484991 PE=4 SV=1	[more]

BLAST of CmoCh03G008320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950348.1	6.3e-100	76.58	protein NEN4-like [Cucurbita moschata]	[more]
KAG6604020.1	3.5e-98	75.09	Protein NEN4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022978542.1	7.7e-98	75.09	protein NEN4-like [Cucurbita maxima]	[more]
KAG7034184.1	2.9e-97	74.72	Protein NEN4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023544691.1	9.4e-96	73.98	protein NEN4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh03G008320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39810.1	4.1e-49	45.90	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	7.8e-32	39.73	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G07710.1	5.1e-31	41.71	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	1.3e-29	37.44	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	7.9e-24	35.71	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 6..80 e-value: 1.8E-9 score: 38.4
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..86 e-value: 6.1E-9 score: 37.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 137..162
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 79..206
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 79..206
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 2..78
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 2..78
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 4..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G008320.1	CmoCh03G008320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding