CmoCh03G008250 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G008250
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	E3 ubiquitin-protein ligase RHA2B-like
Location	Cmo_Chr03: 6606729 .. 6607425 (-)
RNA-Seq Expression	CmoCh03G008250
Synteny	CmoCh03G008250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGTTTTTCTAGGGCGAAATACCAATTTCATTTTGTTCATCACAGAATAGAATCAAATACCATAGAATCTTGATTTTCTGTATTAACTAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

mRNA sequence

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

Coding sequence (CDS)

ATGGGAGCTCTCTCCGAGTTCTTCTCCCACCTCCACACAATGACCGCAGTCTTCATCACCCTTTTACTTCTCGAATGCGTTACTCTCATTCGCACCATCTTTGGTTTGAGACCTAACGCCGACAAGCGCGTAATTACGACGGCGCAGTTTTTGAAATTGATCGAGGAGAAGAATCCGACCATCCGATTCTCCAAGAAAGTTACCTCGAGTTCGGACGATCAGTGCGCCGTTTGCCTCTCTGAATTTGAGGAAGGGGAGATGGTACGGAAATTGCAATGCAACCATACTTTTCACAAGGATTGCCTCGATAATTGGTTGAAACTCTTTTTTGCTACCTGTCCGCTTTGCCGGTGTAAGGTTTTGCCGGACGATATTGTTGCCGGTTACCGCAGGCTGCGAGACCGTGTAGAGTACGACGGCAGCGACGAGGAGCTGATCTTTTTGTTATCGGCATTACATGGTAACAGTATATATAGGAAATATTGTTTTCTCCTTTCACTGACGAACAATAGCGTTAGGGAACAGCTCGTTTCTGGTTCGATTGATCAGGAGCCAGATCGCGATAAATCGTGTATGCTTCTCTTGCAATTACGCAAGCCAACTGTTTGA

Protein sequence

MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRKYCFLLSLTNNSVREQLVSGSIDQEPDRDKSCMLLLQLRKPTV

Homology

[Close]

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot
Match: Q9ZT50 (E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-13
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.5e-13
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-12
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 0


Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.6e-12
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

[Close]

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.6e-11
Identity = 34/76 (44.74%), Postives = 45/76 (59.21%), Query Frame = 0


Query: 53  LIEEKNPTIRFSKKVTSSSD--DQCAVCLSEFEEGEMVRKLQ-CNHTFHKDCLDNWLKLF 112
           LI E  P I+F +   S  D  + CAVCL EFE  + +R L+ C H FH+ CLD W+   
Sbjct: 70  LIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRWMDHD 129

Query: 113 FATCPLCRCKVLPDDI 126
             TCPLCR   +PD++
Sbjct: 130 QKTCPLCRTPFVPDEM 145

[Close]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL
Match: A0A6J1GGU0 (E3 ubiquitin-protein ligase RHA2B-like OS=Cucurbita moschata OX=3662 GN=LOC111453778 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-81
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV
Sbjct: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL
Match: A0A6J1IQI4 (E3 ubiquitin-protein ligase RHA2B-like OS=Cucurbita maxima OX=3661 GN=LOC111478510 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.8e-81
Identity = 152/153 (99.35%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF+TLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFVTLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV
Sbjct: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL
Match: A0A5A7SYH1 (Putative E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002540 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.1e-76
Identity = 146/159 (91.82%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNS+YR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSMYR 159

[Close]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL
Match: A0A6J1HG49 (E3 ubiquitin-protein ligase RHA2B-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111463642 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 6.7e-76
Identity = 144/159 (90.57%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLL+LE V L+RTIFGLRPN DKRVI TAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLILELVILVRTIFGLRPNVDKRVIPTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS K+TSS  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKITSSPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

[Close]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL
Match: A0A0A0KM08 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G501220 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.5e-75
Identity = 145/159 (91.19%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPN+DKRVITTAQF KLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNSDKRVITTAQFFKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: KAG7034177.1 (E3 ubiquitin-protein ligase RHA2B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 317.8 bits (813), Expect = 6.9e-83
Identity = 155/160 (96.88%), Postives = 157/160 (98.12%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV
Sbjct: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYRK 161
           LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHG  + R+
Sbjct: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGGDVRRR 160

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: XP_022950774.1 (E3 ubiquitin-protein ligase RHA2B-like [Cucurbita moschata] >XP_023543925.1 E3 ubiquitin-protein ligase RHA2B-like [Cucurbita pepo subsp. pepo] >KAG6604013.1 E3 ubiquitin-protein ligase RHA2B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.4 bits (799), Expect = 2.9e-81
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV
Sbjct: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: XP_022978570.1 (E3 ubiquitin-protein ligase RHA2B-like [Cucurbita maxima])

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-81
Identity = 152/153 (99.35%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF+TLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFVTLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV
Sbjct: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 154
           LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH
Sbjct: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALH 153

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: KAA0036272.1 (putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa] >TYK12666.1 putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa])

HSP 1 Score: 293.9 bits (751), Expect = 1.1e-75
Identity = 146/159 (91.82%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLLLLE V LIRTIFGLRPNADKRVITTAQFLKLIE+KNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLLLEFVILIRTIFGLRPNADKRVITTAQFLKLIEDKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS KVT S  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKVTPSIADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNS+YR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSMYR 159

[Close]

BLAST of CmoCh03G008250 vs. NCBI nr
Match: XP_022963436.1 (E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita moschata] >XP_023517189.1 E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita pepo subsp. pepo] >KAG7026818.1 E3 ubiquitin-protein ligase RHA2B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293.5 bits (750), Expect = 1.4e-75
Identity = 144/159 (90.57%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MGALSEFFSHLHTMTAVFITLLLLECVTLIRTIFGLRPNADKRVITTAQFLKLIEEKNPT 60
           MGALSEFFSHLHTMTAVF TLL+LE V L+RTIFGLRPN DKRVI TAQFLKLIEEKNPT
Sbjct: 1   MGALSEFFSHLHTMTAVFFTLLILELVILVRTIFGLRPNVDKRVIPTAQFLKLIEEKNPT 60

Query: 61  IRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKV 120
           IRFS K+TSS  DQCAVCLSEFEEGE VRKLQCNHTFHKDCLDNWLKL FATCPLCR KV
Sbjct: 61  IRFSNKITSSPADQCAVCLSEFEEGEKVRKLQCNHTFHKDCLDNWLKLCFATCPLCRSKV 120

Query: 121 LPDDIVAGYRRLRDRVEYDGSDEELIFLLSALHGNSIYR 160
           LPDDIVAGY RLRDRVEYDGSDEELIFLLSALHGNSIYR
Sbjct: 121 LPDDIVAGYHRLRDRVEYDGSDEELIFLLSALHGNSIYR 159

[Close]

BLAST of CmoCh03G008250 vs. TAIR 10
Match: AT1G15100.1 (RING-H2 finger A2A )

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-14
Identity = 32/57 (56.14%), Postives = 40/57 (70.18%), Query Frame = 0


Query: 75  CAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLPDDIVAGYRR 132
           C VCLS+ +EGE VRKL+C H FHK CL+ WL  F  TCPLCR  ++ DD V+  +R
Sbjct: 86  CVVCLSKLKEGEEVRKLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDDCVSKTQR 142

[Close]

BLAST of CmoCh03G008250 vs. TAIR 10
Match: AT2G04240.1 (RING/U-box superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.5e-14
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CmoCh03G008250 vs. TAIR 10
Match: AT2G04240.2 (RING/U-box superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.5e-14
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 41  DKRVITTAQFLKLIEEKNPTIRFSK--KVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFH 100
           D RV     +L+    + PT+RF    +    +D++C+VCLS+F+    + KL+C H FH
Sbjct: 67  DFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLKCGHLFH 126

Query: 101 KDCLDNWLKLFFATCPLCRCK--VLPDD 125
           K CL+ W+  +  TCPLCR    V+P+D
Sbjct: 127 KTCLEKWIDYWNITCPLCRTPLVVVPED 154

[Close]

BLAST of CmoCh03G008250 vs. TAIR 10
Match: AT2G01150.1 (RING-H2 finger protein 2B )

HSP 1 Score: 75.1 bits (183), Expect = 7.2e-14
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 0


Query: 21  LLLLECVTLIRTIFGLR--PNADKRVITTAQFLKLIEEKNPTIRFSKKVTSSSDDQCAVC 80
           +LL    T  R +  L   P++   V+ T+    L ++ N    FS + + ++   C VC
Sbjct: 18  MLLALLATFFRHVRSLLLFPSSAPVVVVTSNLSVLADQLNLNRLFSYRYSDNAASDCIVC 77

Query: 81  LSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRCKVLP 123
           LS+ + GE VRKL C H FHK CL+ WL+     CPLCR  +LP
Sbjct: 78  LSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of CmoCh03G008250 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.7e-13
Identity = 30/66 (45.45%), Postives = 46/66 (69.70%), Query Frame = 0


Query: 59  PTIRFSKKVTSSSDDQCAVCLSEFEEGEMVRKLQCNHTFHKDCLDNWLKLFFATCPLCRC 118
           PT++ +K +  S  +QCAVC+ EFE+G  V+++ C H FH+DCL  WL+L   +CP+CR 
Sbjct: 200 PTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL-HNSCPVCRF 259

Query: 119 KVLPDD 125
           ++  DD
Sbjct: 260 ELPTDD 264

The following BLAST results are available for this feature:

BLAST of CmoCh03G008250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT50	2.0e-13	56.14	E3 ubiquitin-protein ligase RHA2A OS=Arabidopsis thaliana OX=3702 GN=RHA2A PE=1 ...	[more]
Q9SI09	3.5e-13	38.64	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]
Q9ZU51	1.0e-12	37.50	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]
Q8LPN7	6.6e-12	45.45	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q9XF92	5.6e-11	44.74	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]

BLAST of CmoCh03G008250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GGU0	1.4e-81	100.00	E3 ubiquitin-protein ligase RHA2B-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1IQI4	1.8e-81	99.35	E3 ubiquitin-protein ligase RHA2B-like OS=Cucurbita maxima OX=3661 GN=LOC1114785...	[more]
A0A5A7SYH1	5.1e-76	91.82	Putative E3 ubiquitin-protein ligase RHA2B OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A6J1HG49	6.7e-76	90.57	E3 ubiquitin-protein ligase RHA2B-like isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A0A0KM08	1.5e-75	91.19	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G501220 P...	[more]

BLAST of CmoCh03G008250 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034177.1	6.9e-83	96.88	E3 ubiquitin-protein ligase RHA2B [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022950774.1	2.9e-81	100.00	E3 ubiquitin-protein ligase RHA2B-like [Cucurbita moschata] >XP_023543925.1 E3 u...	[more]
XP_022978570.1	3.8e-81	99.35	E3 ubiquitin-protein ligase RHA2B-like [Cucurbita maxima]	[more]
KAA0036272.1	1.1e-75	91.82	putative E3 ubiquitin-protein ligase RHA2B [Cucumis melo var. makuwa] >TYK12666....	[more]
XP_022963436.1	1.4e-75	90.57	E3 ubiquitin-protein ligase RHA2B-like isoform X1 [Cucurbita moschata] >XP_02351...	[more]

BLAST of CmoCh03G008250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15100.1	1.5e-14	56.14	RING-H2 finger A2A	[more]
AT2G04240.1	2.5e-14	38.64	RING/U-box superfamily protein	[more]
AT2G04240.2	2.5e-14	38.64	RING/U-box superfamily protein	[more]
AT2G01150.1	7.2e-14	37.50	RING-H2 finger protein 2B	[more]
AT3G19950.1	4.7e-13	45.45	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 75..116 e-value: 2.4E-7 score: 40.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 73..117 e-value: 6.8E-13 score: 48.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 75..117 score: 12.799091
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 53..124 e-value: 4.9E-21 score: 75.7
None	No IPR available	PANTHER	PTHR47662	FAMILY NOT NAMED	coord: 1..158
None	No IPR available	PANTHER	PTHR47662:SF1	E3 UBIQUITIN-PROTEIN LIGASE XERICO-RELATED	coord: 1..158
None	No IPR available	CDD	cd16486	mRING-H2-C3H2C2D_ZSWM2	coord: 74..117 e-value: 1.41865E-17 score: 71.1037
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 64..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G008250.1	CmoCh03G008250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane