CmoCh03G007740 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G007740
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	DNA-3-methyladenine glycosylase 1-like
Location	Cmo_Chr03: 6410450 .. 6411430 (-)
RNA-Seq Expression	CmoCh03G007740
Synteny	CmoCh03G007740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATATCGTCTCAACCAATCGTAACGCGACGTCTGTACATCACTCCACCTGACTTCCTCTTCAAAACCCAGCTTCTACCCACAAATCATTTACCACCGCCGATGGCCAGAAGAACCCGCCGGAAGTTACTCTTACAGTCCGAGTCTCAAACCGACGCCGATCCACCGTCTAATATTTCGTTTCGAACTACAAAAATACGGAAGATTTCTTCCACTCAAAAATCGGACAAACCACAGATATCAACTCCTGGCGGAGGCGACCGGACTCGAGCATTCCCGAACCAGGATGGTCCTGTCAAATCTTTATCGTCTTCGGATGTAATTTGTACAGCGATCGATCATTTACGCCGTTCGGATCCCCTTCTGATAAGGCTATTAGATTCATGCGAATCCCCCAATTTCAAGTCCAATCCACCGTTTCTAGCCCTAACAAAGAGCATCCTCTACCAGCAGCTCGCTACGAAGGCCGCCGAATCGATCTACAATCGCTTCGCGTCGCTATGCGGCGGAGATGCGGCAGTACTACCGGACGCCGTACTTGGACTCTCGCCTCAACAGCTGCGAGTGGTCGGAGTTTCGGGTAGAAAAGCAAGTTACCTTCATGACCTAGCGACCAAATTCATAGAGGGCAGTTTGTCGAATTCATCGATTCTAGAGATGGACGATGAGACTCTACTGAGTGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCAGTGCACATGTTCATGATTTTTACTCTGCACCGGCCGGATGTGCTGCCGGTGGGGGATTTGGGCGTGAGAAAAGGGGTGCAGAGGTTGTACGGACTGAAAGAATTGCCAAAGCCAGTGGAGATGGAGAAACTTTGTGAAAAATGGAAGCCGTACAGGTCGATGGGGGCTTGGTATATGTGGAGGCTGATGGAAATGAAGGAAATCGTGAAGGATGATGGGGATTTGAAGATGAACACGGCGAACGGCGGCGGCGTTGTAATGTGA

mRNA sequence

ATGATATCGTCTCAACCAATCGTAACGCGACGTCTGTACATCACTCCACCTGACTTCCTCTTCAAAACCCAGCTTCTACCCACAAATCATTTACCACCGCCGATGGCCAGAAGAACCCGCCGGAAGTTACTCTTACAGTCCGAGTCTCAAACCGACGCCGATCCACCGTCTAATATTTCGTTTCGAACTACAAAAATACGGAAGATTTCTTCCACTCAAAAATCGGACAAACCACAGATATCAACTCCTGGCGGAGGCGACCGGACTCGAGCATTCCCGAACCAGGATGGTCCTGTCAAATCTTTATCGTCTTCGGATGTAATTTGTACAGCGATCGATCATTTACGCCGTTCGGATCCCCTTCTGATAAGGCTATTAGATTCATGCGAATCCCCCAATTTCAAGTCCAATCCACCGTTTCTAGCCCTAACAAAGAGCATCCTCTACCAGCAGCTCGCTACGAAGGCCGCCGAATCGATCTACAATCGCTTCGCGTCGCTATGCGGCGGAGATGCGGCAGTACTACCGGACGCCGTACTTGGACTCTCGCCTCAACAGCTGCGAGTGGTCGGAGTTTCGGGTAGAAAAGCAAGTTACCTTCATGACCTAGCGACCAAATTCATAGAGGGCAGTTTGTCGAATTCATCGATTCTAGAGATGGACGATGAGACTCTACTGAGTGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCAGTGCACATGTTCATGATTTTTACTCTGCACCGGCCGGATGTGCTGCCGGTGGGGGATTTGGGCGTGAGAAAAGGGGTGCAGAGGTTGTACGGACTGAAAGAATTGCCAAAGCCAGTGGAGATGGAGAAACTTTGTGAAAAATGGAAGCCGTACAGGTCGATGGGGGCTTGGTATATGTGGAGGCTGATGGAAATGAAGGAAATCGTGAAGGATGATGGGGATTTGAAGATGAACACGGCGAACGGCGGCGGCGTTGTAATGTGA

Coding sequence (CDS)

ATGATATCGTCTCAACCAATCGTAACGCGACGTCTGTACATCACTCCACCTGACTTCCTCTTCAAAACCCAGCTTCTACCCACAAATCATTTACCACCGCCGATGGCCAGAAGAACCCGCCGGAAGTTACTCTTACAGTCCGAGTCTCAAACCGACGCCGATCCACCGTCTAATATTTCGTTTCGAACTACAAAAATACGGAAGATTTCTTCCACTCAAAAATCGGACAAACCACAGATATCAACTCCTGGCGGAGGCGACCGGACTCGAGCATTCCCGAACCAGGATGGTCCTGTCAAATCTTTATCGTCTTCGGATGTAATTTGTACAGCGATCGATCATTTACGCCGTTCGGATCCCCTTCTGATAAGGCTATTAGATTCATGCGAATCCCCCAATTTCAAGTCCAATCCACCGTTTCTAGCCCTAACAAAGAGCATCCTCTACCAGCAGCTCGCTACGAAGGCCGCCGAATCGATCTACAATCGCTTCGCGTCGCTATGCGGCGGAGATGCGGCAGTACTACCGGACGCCGTACTTGGACTCTCGCCTCAACAGCTGCGAGTGGTCGGAGTTTCGGGTAGAAAAGCAAGTTACCTTCATGACCTAGCGACCAAATTCATAGAGGGCAGTTTGTCGAATTCATCGATTCTAGAGATGGACGATGAGACTCTACTGAGTGCGTTAACGGCGGTGAAGGGAATCGGCGTTTGGTCAGTGCACATGTTCATGATTTTTACTCTGCACCGGCCGGATGTGCTGCCGGTGGGGGATTTGGGCGTGAGAAAAGGGGTGCAGAGGTTGTACGGACTGAAAGAATTGCCAAAGCCAGTGGAGATGGAGAAACTTTGTGAAAAATGGAAGCCGTACAGGTCGATGGGGGCTTGGTATATGTGGAGGCTGATGGAAATGAAGGAAATCGTGAAGGATGATGGGGATTTGAAGATGAACACGGCGAACGGCGGCGGCGTTGTAATGTGA

Protein sequence

MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNISFRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGDLKMNTANGGGVVM

Homology

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BLAST of CmoCh03G007740 vs. ExPASy Swiss-Prot
Match: Q92383 (DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mag1 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.8e-25
Identity = 64/213 (30.05%), Postives = 116/213 (54.46%), Query Frame = 0


Query: 99  VKSLSSSDVICTAIDHLRRSDPLLIRLLDSCE-SPNFKSNPPFLALTKSILYQQLATKAA 158
           V SL+ +++  + +D   +    L++L+ +   + + +   P+  L +++  QQL +KAA
Sbjct: 12  VTSLTKAEIHLSGLDENWKR---LVKLVGNYRPNRSMEKKEPYEELIRAVASQQLHSKAA 71

Query: 159 ESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSI 218
            +I+NRF S+        P+ +  +  + +R  G S RK   L  +A   I G +     
Sbjct: 72  NAIFNRFKSISNNGQFPTPEEIRDMDFEIMRACGFSARKIDSLKSIAEATISGLIPTKEE 131

Query: 219 LE-MDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPK 278
            E + +E L+  LT +KGIG W+V M +IF+L+R DV+P  DL +R G + L+ L ++P 
Sbjct: 132 AERLSNEELIERLTQIKGIGRWTVEMLLIFSLNRDDVMPADDLSIRNGYRYLHRLPKIPT 191

Query: 279 PVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVK 310
            + + K  E   P+R+  AWY+W+  ++ +  K
Sbjct: 192 KMYVLKHSEICAPFRTAAAWYLWKTSKLADYTK 221

[Close]

BLAST of CmoCh03G007740 vs. ExPASy Swiss-Prot
Match: O94468 (Alkylbase DNA glycosidase-like protein mag2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mag2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.3e-22
Identity = 54/185 (29.19%), Postives = 100/185 (54.05%), Query Frame = 0


Query: 122 LIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCG-GDAAVLPDAVL 181
           L++ +  C       + P+  + ++I  Q+L+  A  SI N+F + C   D    P  ++
Sbjct: 24  LVKKVGPCTLTPHPEHAPYEGIIRAITSQKLSDAATNSIINKFCTQCSDNDEFPTPKQIM 83

Query: 182 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSL-SNSSILEMDDETLLSALTAVKGIGVWS 241
               + L   G S  K+  +H +A   +   + S S I +M +E L+ +L+ +KG+  W+
Sbjct: 84  ETDVETLHECGFSKLKSQEIHIVAEAALNKQIPSKSEIEKMSEEELMESLSKIKGVKRWT 143

Query: 242 VHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMW 301
           + M+ IFTL R D++P  D  ++   +  +GL   P+  E+EKL +  KPYR++ AWY+W
Sbjct: 144 IEMYSIFTLGRLDIMPADDSTLKNEAKEFFGLSSKPQTEEVEKLTKPCKPYRTIAAWYLW 203

Query: 302 RLMEM 305
           ++ ++
Sbjct: 204 QIPKL 208

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BLAST of CmoCh03G007740 vs. ExPASy Swiss-Prot
Match: O31544 (Putative DNA-3-methyladenine glycosylase YfjP OS=Bacillus subtilis (strain 168) OX=224308 GN=yfjP PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.4e-19
Identity = 53/166 (31.93%), Postives = 89/166 (53.61%), Query Frame = 0


Query: 143 LTKSILYQQLATKAAESIYNRFASLCGGDAAVL-----PDAVLGLSPQQLRVVGVSGRKA 202
           + K I++QQL    A ++  RF    G     +     P+ +  L  Q LR +  S RKA
Sbjct: 122 MMKCIIHQQLNLSFAYTLTERFVHAFGEQKDGVWCYPKPETIAELDYQDLRDLQFSMRKA 181

Query: 203 SYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVG 262
            Y  D +    EG+LS S +  M DE ++  L  ++GIG W+V   ++F L RP++ P+ 
Sbjct: 182 EYTIDTSRMIAEGTLSLSELPHMADEDIMKKLIKIRGIGPWTVQNVLMFGLGRPNLFPLA 241

Query: 263 DLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLME 304
           D+G++  ++R + L + P    M  + ++W+PY S  + Y+WR +E
Sbjct: 242 DIGLQNAIKRHFQLDDKPAKDVMLAMSKEWEPYLSYASLYLWRSIE 287

[Close]

BLAST of CmoCh03G007740 vs. ExPASy Swiss-Prot
Match: P22134 (DNA-3-methyladenine glycosylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAG1 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 7.9e-15
Identity = 74/232 (31.90%), Postives = 104/232 (44.83%), Query Frame = 0


Query: 111 AIDHLRRSDPLLIRLLDSCESPNF--KSNPP------FLALTKSILYQQLATKAAESIYN 170
           A +H+   DP L  +L + E   +  ++  P      F+ L  +IL QQ++ +AAESI  
Sbjct: 47  ACEHILEKDPSLFPILKNNEFTLYLKETQVPNTLEDYFIRLASTILSQQISGQAAESIKA 106

Query: 171 RFASLCGG---DAAVL-PDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSIL 230
           R  SL GG   D  +L  D        ++   G+S RK  YL  LA  F E       + 
Sbjct: 107 RVVSLYGGAFPDYKILFEDFKDPAKCAEIAKCGLSKRKMIYLESLAVYFTEKYKDIEKLF 166

Query: 231 ---EMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLK-EL 290
              + D+E + S +T VKGIG WS  MF+I  L R DV    DLG+ +G  +    K EL
Sbjct: 167 GQKDNDEEVIESLVTNVKGIGPWSAKMFLISGLKRMDVFAPEDLGIARGFSKYLSDKPEL 226

Query: 291 PKPVE-------------------------MEKLCEKWKPYRSMGAWYMWRL 302
            K +                          MEK  E + PYRS+  + +WRL
Sbjct: 227 EKELMRERKVVKKSKIKHKKYNWKIYDDDIMEKCSETFSPYRSVFMFILWRL 278

[Close]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL
Match: A0A6J1GD23 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453068 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.8e-183
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS
Sbjct: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 327
           LMEMKEIVKDDGDLKMNTANGGGVVM
Sbjct: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 326

[Close]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL
Match: A0A6J1IQD1 (DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478481 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.4e-170
Identity = 303/323 (93.81%), Postives = 312/323 (96.59%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPI TRRLY TPP+ LFKTQLL TNHLPPPMARRTRRKLLLQSESQT+ADPPS IS
Sbjct: 1   MISSQPITTRRLYFTPPESLFKTQLLHTNHLPPPMARRTRRKLLLQSESQTEADPPSKIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTT+IRKISST+K DKPQIST GGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 61  FRTTEIRKISSTRKPDKPQISTDGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLA+TKSILYQQLATKAAESIYNRFASLCGG+AAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLAITKSILYQQLATKAAESIYNRFASLCGGEAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKF+EG+LSNSSILEMDDETLLSALT VKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFVEGTLSNSSILEMDDETLLSALTGVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGG 324
           LMEMK I K+DGDLK NTANGGG
Sbjct: 301 LMEMKGIAKNDGDLKKNTANGGG 323

[Close]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL
Match: A0A0A0KM62 (ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.3e-124
Identity = 234/284 (82.39%), Postives = 249/284 (87.68%), Query Frame = 0


Query: 35  MARRTRRKLLLQSESQTDADP-----PSNISFRTTKIRKISSTQKSDKPQISTPGGGDRT 94
           MA+R RRK L Q ES +DA P      S I F +TK+RKISS Q+  KPQIS PGG + T
Sbjct: 1   MAKRIRRKCLFQLESPSDAVPLSPSASSKIPFPSTKVRKISSNQEPTKPQISAPGGYNPT 60

Query: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154
           R FPN   PVKSLSSSD I TAI+HLRRSDPLLI LLDSCE+PNFKSNPPFLALTKSILY
Sbjct: 61  RIFPNLADPVKSLSSSDKISTAINHLRRSDPLLISLLDSCETPNFKSNPPFLALTKSILY 120

Query: 155 QQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214
           QQLATKAAE+IYNRFASLCGG+AAVLPD VLGLSPQQLRV+GVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAEAIYNRFASLCGGEAAVLPDTVLGLSPQQLRVIGVSGRKASYLHDLATKFIE 180

Query: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274
           GSLSNS ILEMDDETLL ALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GSLSNSFILEMDDETLLRALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGD 314
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRL++ KEIVK+  D
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLIDAKEIVKNGCD 284

[Close]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL
Match: A0A5A7T3R0 (Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002080 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 236/293 (80.55%), Postives = 248/293 (84.64%), Query Frame = 0


Query: 35  MARRTRRKLLLQSESQTDADP-----PSNISFRTTKIRKISSTQKSDKPQISTPGGGDRT 94
           MA+R RRK L QSES T A P      S I FR+TK+RKISS Q+  KPQ S P G + T
Sbjct: 1   MAKRIRRKFLFQSESPTGAVPLSPSSSSKIPFRSTKVRKISSNQEPAKPQFSAPDGYNPT 60

Query: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154
           R FPN   PVKSLSS D I TAI+HLRRSDPLLI LLDSCESP+FKSNPPFLALTKSILY
Sbjct: 61  RTFPNLADPVKSLSSLDEISTAINHLRRSDPLLISLLDSCESPHFKSNPPFLALTKSILY 120

Query: 155 QQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214
           QQLATKAAESIYNRFASLCGG+A+VLPD VLGLSPQQLRVVGVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEASVLPDTVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 180

Query: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274
           G+LSNS ILEMDDETLL  LTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSLILEMDDETLLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGDLKMNTANGG 323
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRLME K +VK   DL  N  N G
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLMEEKGVVKKGSDLPDNMENRG 293

[Close]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL
Match: A0A1S3B2D5 (probable DNA-3-methyladenine glycosylase 2 OS=Cucumis melo OX=3656 GN=LOC103485049 PE=4 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.6e-124
Identity = 236/293 (80.55%), Postives = 248/293 (84.64%), Query Frame = 0


Query: 35  MARRTRRKLLLQSESQTDADP-----PSNISFRTTKIRKISSTQKSDKPQISTPGGGDRT 94
           MA+R RRK L QSES T A P      S I FR+TK+RKISS Q+  KPQ S P G + T
Sbjct: 1   MAKRIRRKFLFQSESPTGAVPLSPSSSSKIPFRSTKVRKISSNQEPAKPQFSAPDGYNPT 60

Query: 95  RAFPNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKSNPPFLALTKSILY 154
           R FPN   PVKSLSS D I TAI+HLRRSDPLLI LLDSCESP+FKSNPPFLALTKSILY
Sbjct: 61  RTFPNLADPVKSLSSLDEISTAINHLRRSDPLLISLLDSCESPHFKSNPPFLALTKSILY 120

Query: 155 QQLATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 214
           QQLATKAAESIYNRFASLCGG+A+VLPD VLGLSPQQLRVVGVSGRKASYLHDLATKFIE
Sbjct: 121 QQLATKAAESIYNRFASLCGGEASVLPDTVLGLSPQQLRVVGVSGRKASYLHDLATKFIE 180

Query: 215 GSLSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 274
           G+LSNS ILEMDDETLL  LTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY
Sbjct: 181 GNLSNSLILEMDDETLLGELTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLY 240

Query: 275 GLKELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMKEIVKDDGDLKMNTANGG 323
           GLKELPKP EMEKLCEKWKPYRS+GAWYMWRLME K +VK   DL  N  N G
Sbjct: 241 GLKELPKPAEMEKLCEKWKPYRSIGAWYMWRLMEEKGVVKKGSDLPDNMENRG 293

[Close]

BLAST of CmoCh03G007740 vs. NCBI nr
Match: XP_022949777.1 (DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS
Sbjct: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 327
           LMEMKEIVKDDGDLKMNTANGGGVVM
Sbjct: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 326

[Close]

BLAST of CmoCh03G007740 vs. NCBI nr
Match: KAG6603971.1 (Zinc-finger homeodomain protein 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 649.4 bits (1674), Expect = 1.6e-182
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS
Sbjct: 250 MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 309

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 310 FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 369

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL
Sbjct: 370 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 429

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV
Sbjct: 430 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 489

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 490 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 549

Query: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 327
           LMEMKEIVKDDGDLKMNTANGGGVVM
Sbjct: 550 LMEMKEIVKDDGDLKMNTANGGGVVM 575

[Close]

BLAST of CmoCh03G007740 vs. NCBI nr
Match: KAG7034142.1 (mag1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647.1 bits (1668), Expect = 8.0e-182
Identity = 325/326 (99.69%), Postives = 325/326 (99.69%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTD DPPSNIS
Sbjct: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDDDPPSNIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 327
           LMEMKEIVKDDGDLKMNTANGGGVVM
Sbjct: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 326

[Close]

BLAST of CmoCh03G007740 vs. NCBI nr
Match: XP_023543059.1 (DNA-3-methyladenine glycosylase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 621.3 bits (1601), Expect = 4.7e-174
Identity = 313/326 (96.01%), Postives = 318/326 (97.55%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPI+TRRLY TP + L KTQ+LPTNHLPPPMARRTRRKLLLQSESQT+ADPPS IS
Sbjct: 1   MISSQPIITRRLYCTPLESLLKTQVLPTNHLPPPMARRTRRKLLLQSESQTEADPPSKIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTTKIRKISSTQK DKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVI TAIDHLRRSDP
Sbjct: 61  FRTTKIRKISSTQKPDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVIRTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGG+AAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGEAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCE WKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCENWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 327
           LMEMKEIVKDDGDLKMNTANGGGVVM
Sbjct: 301 LMEMKEIVKDDGDLKMNTANGGGVVM 326

[Close]

BLAST of CmoCh03G007740 vs. NCBI nr
Match: XP_022978525.1 (DNA-3-methyladenine glycosylase 1-like [Cucurbita maxima])

HSP 1 Score: 607.4 bits (1565), Expect = 7.0e-170
Identity = 303/323 (93.81%), Postives = 312/323 (96.59%), Query Frame = 0


Query: 1   MISSQPIVTRRLYITPPDFLFKTQLLPTNHLPPPMARRTRRKLLLQSESQTDADPPSNIS 60
           MISSQPI TRRLY TPP+ LFKTQLL TNHLPPPMARRTRRKLLLQSESQT+ADPPS IS
Sbjct: 1   MISSQPITTRRLYFTPPESLFKTQLLHTNHLPPPMARRTRRKLLLQSESQTEADPPSKIS 60

Query: 61  FRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120
           FRTT+IRKISST+K DKPQIST GGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP
Sbjct: 61  FRTTEIRKISSTRKPDKPQISTDGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLRRSDP 120

Query: 121 LLIRLLDSCESPNFKSNPPFLALTKSILYQQLATKAAESIYNRFASLCGGDAAVLPDAVL 180
           LLIRLLDSCESPNFKSNPPFLA+TKSILYQQLATKAAESIYNRFASLCGG+AAVLPDAVL
Sbjct: 121 LLIRLLDSCESPNFKSNPPFLAITKSILYQQLATKAAESIYNRFASLCGGEAAVLPDAVL 180

Query: 181 GLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVKGIGVWSV 240
           GLSPQQLRVVGVSGRKASYLHDLATKF+EG+LSNSSILEMDDETLLSALT VKGIGVWSV
Sbjct: 181 GLSPQQLRVVGVSGRKASYLHDLATKFVEGTLSNSSILEMDDETLLSALTGVKGIGVWSV 240

Query: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300
           HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR
Sbjct: 241 HMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSMGAWYMWR 300

Query: 301 LMEMKEIVKDDGDLKMNTANGGG 324
           LMEMK I K+DGDLK NTANGGG
Sbjct: 301 LMEMKGIAKNDGDLKKNTANGGG 323

[Close]

BLAST of CmoCh03G007740 vs. TAIR 10
Match: AT1G19480.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 5.5e-72
Identity = 146/274 (53.28%), Postives = 184/274 (67.15%), Query Frame = 0


Query: 53  ADPPSNISFRTTKIRKIS---------------STQKSDKPQIS---TPGGG--DRTRAF 112
           + PPS I  R  KIRK++               S+ + + P  +   +PG G     RA 
Sbjct: 64  SSPPSKIPLRPRKIRKLTLDGDVSGEDYKAEDISSSQVNSPLATDGKSPGKGKLSHLRAI 123

Query: 113 PNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKS-NPPFLALTKSILYQQ 172
                  + L+    + TAI +LR +DPLL  L+D    P F+S   PFLAL ++ILYQQ
Sbjct: 124 TVPRIQARPLTCEGELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQ 183

Query: 173 LATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGS 232
           LA KA  SIY RF SLCGG+  V+P+ VL L+PQQLR +GVSGRKASYLHDLA K+  G 
Sbjct: 184 LAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGI 243

Query: 233 LSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL 292
           LS+S+IL MD+++L + LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ LYGL
Sbjct: 244 LSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

Query: 293 KELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMK 306
            +LP+P +ME+ C KW+PYRS+G+WYMWRL+E K
Sbjct: 304 DDLPRPSQMEQHCAKWRPYRSVGSWYMWRLIEAK 337

[Close]

BLAST of CmoCh03G007740 vs. TAIR 10
Match: AT1G19480.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 5.5e-72
Identity = 146/274 (53.28%), Postives = 184/274 (67.15%), Query Frame = 0


Query: 53  ADPPSNISFRTTKIRKIS---------------STQKSDKPQIS---TPGGG--DRTRAF 112
           + PPS I  R  KIRK++               S+ + + P  +   +PG G     RA 
Sbjct: 64  SSPPSKIPLRPRKIRKLTLDGDVSGEDYKAEDISSSQVNSPLATDGKSPGKGKLSHLRAI 123

Query: 113 PNQDGPVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKS-NPPFLALTKSILYQQ 172
                  + L+    + TAI +LR +DPLL  L+D    P F+S   PFLAL ++ILYQQ
Sbjct: 124 TVPRIQARPLTCEGELETAIHYLRNADPLLAALIDVHPPPTFESFKTPFLALIRNILYQQ 183

Query: 173 LATKAAESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGS 232
           LA KA  SIY RF SLCGG+  V+P+ VL L+PQQLR +GVSGRKASYLHDLA K+  G 
Sbjct: 184 LAMKAGNSIYTRFVSLCGGENLVVPETVLSLNPQQLRQIGVSGRKASYLHDLARKYQNGI 243

Query: 233 LSNSSILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGL 292
           LS+S+IL MD+++L + LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ LYGL
Sbjct: 244 LSDSAILNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQLLYGL 303

Query: 293 KELPKPVEMEKLCEKWKPYRSMGAWYMWRLMEMK 306
            +LP+P +ME+ C KW+PYRS+G+WYMWRL+E K
Sbjct: 304 DDLPRPSQMEQHCAKWRPYRSVGSWYMWRLIEAK 337

[Close]

BLAST of CmoCh03G007740 vs. TAIR 10
Match: AT1G75230.2 (DNA glycosylase superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.6e-71
Identity = 141/269 (52.42%), Postives = 179/269 (66.54%), Query Frame = 0


Query: 53  ADPPSNISFRTTKIRKIS---------------STQKSDKPQISTPGGGDRTRAFPNQDG 112
           + PP+ I  R  KIRK+S               S   + KP   +     RT   P    
Sbjct: 67  SSPPTKIPLRPRKIRKLSPDDDASDGFNPEHNLSQMTTTKPATKSKLSQSRTVTVPRIQ- 126

Query: 113 PVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKS-NPPFLALTKSILYQQLATKA 172
             +SL+    +  A+ HLR  DPLL  L+D    P F++   PFLAL +SILYQQLA KA
Sbjct: 127 -ARSLTCEGELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKA 186

Query: 173 AESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSS 232
             SIY RF +LCGG+  V+P+ VL L+PQQLR +GVSGRKASYLHDLA K+  G LS+S 
Sbjct: 187 GNSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSG 246

Query: 233 ILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPK 292
           I+ MD+++L + LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ L G+++LP+
Sbjct: 247 IVNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGMEDLPR 306

Query: 293 PVEMEKLCEKWKPYRSMGAWYMWRLMEMK 306
           P +ME+LCEKW+PYRS+ +WY+WRL+E K
Sbjct: 307 PSKMEQLCEKWRPYRSVASWYLWRLIESK 333

[Close]

BLAST of CmoCh03G007740 vs. TAIR 10
Match: AT1G75230.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.6e-71
Identity = 141/269 (52.42%), Postives = 179/269 (66.54%), Query Frame = 0


Query: 53  ADPPSNISFRTTKIRKIS---------------STQKSDKPQISTPGGGDRTRAFPNQDG 112
           + PP+ I  R  KIRK+S               S   + KP   +     RT   P    
Sbjct: 67  SSPPTKIPLRPRKIRKLSPDDDASDGFNPEHNLSQMTTTKPATKSKLSQSRTVTVPRIQ- 126

Query: 113 PVKSLSSSDVICTAIDHLRRSDPLLIRLLDSCESPNFKS-NPPFLALTKSILYQQLATKA 172
             +SL+    +  A+ HLR  DPLL  L+D    P F++   PFLAL +SILYQQLA KA
Sbjct: 127 -ARSLTCEGELEAALHHLRSVDPLLASLIDIHPPPTFETFQTPFLALIRSILYQQLAAKA 186

Query: 173 AESIYNRFASLCGGDAAVLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSS 232
             SIY RF +LCGG+  V+P+ VL L+PQQLR +GVSGRKASYLHDLA K+  G LS+S 
Sbjct: 187 GNSIYTRFVALCGGENGVVPENVLPLTPQQLRQIGVSGRKASYLHDLARKYQNGILSDSG 246

Query: 233 ILEMDDETLLSALTAVKGIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPK 292
           I+ MD+++L + LT V GIG WSVHMFMI +LHRPDVLPV DLGVRKGVQ L G+++LP+
Sbjct: 247 IVNMDEKSLFTMLTMVNGIGSWSVHMFMINSLHRPDVLPVNDLGVRKGVQMLNGMEDLPR 306

Query: 293 PVEMEKLCEKWKPYRSMGAWYMWRLMEMK 306
           P +ME+LCEKW+PYRS+ +WY+WRL+E K
Sbjct: 307 PSKMEQLCEKWRPYRSVASWYLWRLIESK 333

[Close]

BLAST of CmoCh03G007740 vs. TAIR 10
Match: AT3G50880.1 (DNA glycosylase superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.8e-70
Identity = 142/253 (56.13%), Postives = 177/253 (69.96%), Query Frame = 0


Query: 57  SNISFRTTKIRKISSTQKSDKPQISTPGGGDRTRAFPNQDGPVKSLSSSDVICTAIDHLR 116
           S I FR  KIRK+SS                  R       P+ + S+ D+   A+ HL+
Sbjct: 36  SRIRFRPRKIRKVSSDPS--------------PRIIITASPPLSTKSTVDI---ALRHLQ 95

Query: 117 RSDPLLIRLLDSCESPNF--KSNPPFLALTKSILYQQLATKAAESIYNRFASLC-GGDAA 176
            SD LL  L+ +   P     SN PFL+L +SILYQQLATKAA+ IY+RF SL  GG+A 
Sbjct: 96  SSDELLGALITTHNDPPLFDSSNTPFLSLARSILYQQLATKAAKCIYDRFISLFNGGEAG 155

Query: 177 VLPDAVLGLSPQQLRVVGVSGRKASYLHDLATKFIEGSLSNSSILEMDDETLLSALTAVK 236
           V+P++V+ LS   LR +GVSGRKASYLHDLA K+  G LS+  IL+M DE L+  LT VK
Sbjct: 156 VVPESVISLSAVDLRKIGVSGRKASYLHDLADKYNNGVLSDELILKMSDEELIDRLTLVK 215

Query: 237 GIGVWSVHMFMIFTLHRPDVLPVGDLGVRKGVQRLYGLKELPKPVEMEKLCEKWKPYRSM 296
           GIGVW+VHMFMIF+LHRPDVLPVGDLGVRKGV+ LYGLK LP P++ME+LCEKW+PYRS+
Sbjct: 216 GIGVWTVHMFMIFSLHRPDVLPVGDLGVRKGVKDLYGLKNLPGPLQMEQLCEKWRPYRSV 271

Query: 297 GAWYMWRLMEMKE 307
           G+WYMWRL+E ++
Sbjct: 276 GSWYMWRLIESRK 271

The following BLAST results are available for this feature:

BLAST of CmoCh03G007740 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q92383	3.8e-25	30.05	DNA-3-methyladenine glycosylase 1 OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]
O94468	2.3e-22	29.19	Alkylbase DNA glycosidase-like protein mag2 OS=Schizosaccharomyces pombe (strain...	[more]
O31544	2.4e-19	31.93	Putative DNA-3-methyladenine glycosylase YfjP OS=Bacillus subtilis (strain 168) ...	[more]
P22134	7.9e-15	31.90	DNA-3-methyladenine glycosylase OS=Saccharomyces cerevisiae (strain ATCC 204508 ...	[more]

BLAST of CmoCh03G007740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GD23	7.8e-183	100.00	DNA-3-methyladenine glycosylase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1IQD1	3.4e-170	93.81	DNA-3-methyladenine glycosylase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114784...	[more]
A0A0A0KM62	1.3e-124	82.39	ENDO3c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G502690 PE=4...	[more]
A0A5A7T3R0	1.6e-124	80.55	Putative DNA-3-methyladenine glycosylase 2 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3B2D5	1.6e-124	80.55	probable DNA-3-methyladenine glycosylase 2 OS=Cucumis melo OX=3656 GN=LOC1034850...	[more]

BLAST of CmoCh03G007740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949777.1	1.6e-182	100.00	DNA-3-methyladenine glycosylase 1-like [Cucurbita moschata]	[more]
KAG6603971.1	1.6e-182	100.00	Zinc-finger homeodomain protein 6, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7034142.1	8.0e-182	99.69	mag1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023543059.1	4.7e-174	96.01	DNA-3-methyladenine glycosylase 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022978525.1	7.0e-170	93.81	DNA-3-methyladenine glycosylase 1-like [Cucurbita maxima]	[more]

BLAST of CmoCh03G007740 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19480.1	5.5e-72	53.28	DNA glycosylase superfamily protein	[more]
AT1G19480.2	5.5e-72	53.28	DNA glycosylase superfamily protein	[more]
AT1G75230.2	3.6e-71	52.42	DNA glycosylase superfamily protein	[more]
AT1G75230.1	3.6e-71	52.42	DNA glycosylase superfamily protein	[more]
AT3G50880.1	1.8e-70	56.13	DNA glycosylase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003265	HhH-GPD domain	SMART	SM00478	endo3end	coord: 148..303 e-value: 1.1E-19 score: 81.4
IPR003265	HhH-GPD domain	PFAM	PF00730	HhH-GPD	coord: 145..288 e-value: 3.4E-21 score: 75.7
IPR003265	HhH-GPD domain	CDD	cd00056	ENDO3c	coord: 140..301 e-value: 2.55602E-35 score: 123.891
None	No IPR available	GENE3D	1.10.1670.40		coord: 114..300 e-value: 1.9E-68 score: 231.7
None	No IPR available	GENE3D	1.10.340.30	Hypothetical protein; domain 2	coord: 139..250 e-value: 1.9E-68 score: 231.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..82
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..102
None	No IPR available	PANTHER	PTHR43003:SF8	HHH-GPD BASE EXCISION DNA REPAIR FAMILY PROTEIN	coord: 46..310
None	No IPR available	PANTHER	PTHR43003	DNA-3-METHYLADENINE GLYCOSYLASE	coord: 46..310
IPR011257	DNA glycosylase	SUPERFAMILY	48150	DNA-glycosylase	coord: 137..301

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G007740.1	CmoCh03G007740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006285	base-excision repair, AP site formation
biological_process	GO:0006307	DNA dealkylation involved in DNA repair
biological_process	GO:0006284	base-excision repair
biological_process	GO:0006281	DNA repair
cellular_component	GO:0005634	nucleus
cellular_component	GO:0032993	protein-DNA complex
molecular_function	GO:0032131	alkylated DNA binding
molecular_function	GO:0008725	DNA-3-methyladenine glycosylase activity
molecular_function	GO:0043916	DNA-7-methylguanine glycosylase activity
molecular_function	GO:0003824	catalytic activity