CmoCh03G006860 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G006860
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionaspartyl protease family protein 1-like
LocationCmo_Chr03: 6029438 .. 6032342 (+)
RNA-Seq ExpressionCmoCh03G006860
SyntenyCmoCh03G006860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCTCCGGTCCATGTTACCCTCTCGTCCTCCTGATCCCATTTAAATTCTTCTCCTCAAATCCTCTTCACACCGGAAAACTTTTGGATCCTCTAATCGGCGTCCATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCCGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCAGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGATAACCACACCTACCGATACGGCGGTTTGGGAGAGTATTGCGCCTTACTTTCTCTGTTGATTTTCCGATTTACTTTGGCCGATTGCCCTTGCTTTAATTTGTTTCCTGTTGGCTTTTCAGTTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCGTACTTCCGTGGGCACTTCAGATGGGGAAGATGTATTTTCTTTCCGCCCTCTTCAATTTTCTCGGGAAACAAACAGTGCAAAATTTTGAAGTTGATGTTCTTATTTGTTTATGTTTCTTCAGTTAACGTTGAATCATTACAGTCCAAATGCTTCAACCACGAGCAAAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGGTGAGAACTGTTGAATCTCTCTGTCTGAATCAAATGCTTCTGAATTATGAAGAAATTTCGTTTAGTTCTTAATACATTTCTTTCTGCTAACATAACAAGCTAACCAAATACAAAATCTATGGTAGTTTTACGATGTTTTAGCCGTTTCTAATATAAGTCGTGAAGAATAAGTTCATGAATCTTCTGTGCATGAATGACATTTATTTGGTTTATAACTCTTTTGGTGATACATGTTCATACACTTTGTAACCATTATTAAGATTGGTTGCTATCAATGGATATGGGGAAATTTCTTCCCTTTGAACCATGTAAATCTTTTTGTCTCTTTGTTAAATTTTTGTTTGTGGGTGTGTGAGTAGATCGATCTTGCTTCAGCTTCCGTTACAACAATGTCAACAATACTATCTTTCATAGAAACTATCTGCAATCTGGTGTGTTATTGCGTTGCTTTAAATGGGATTCTTGATGGCAGGTGTGGTAAGGTACAAACTGGTATATTTTCCAGTATCGCAATTAATGGTCTTCTTGGGCTTGGCATGGGGAAGATATCTGTTCCATCCCTCTTAGCAAGCCAAGAGCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCGCTCAATCCCCACTCGTAAGATCTTCTCACCTTTTTAATCTACGACCTTCATTTCTCCTGGATAATAAGCTGTTTTTCCTAGCTGAATTCTGTTGTTTGGTGGCAGTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACCACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTTTCGTTACTCAGAATGTAAGCTATTATCGCTGGGAAGTGTCATACAAGTTCATGGAATCTCTGGTTTATATATAGAATTTTTTGTGCAGATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTCGAGTACTGCTACCAACTTCCGTAAGTAGATGATAACAATTTTTGGTTCTTGACCCTTTTATTATAGACAAATATCTGATATTGTGTTTCTATCTCGTTTGTAATTTATTGGATTCTGTAAATGATTATAAAAAGAGAATTTTCCGTACGTGGGGTGATTTTAGCAAATATTCTCGATAAGATGTTCAGATTCTAATTCCCCTCAATTCCTATCCAGTCTACGAACACTCTTTCAACAACCGCATCTGAATTTTACTATGGATAGTGGCTATATTTTTGGCGTTATTTCTATGTTTGTTGCCATTCCTACCGATGTGAGTTATCTGAACAGTTCAAGTCTTTTGCTTTTTAAGCTTTCTTTCTGTAATCCTTTGGGTTTTTCTTGATCTGGGTGTTCGCGTGGGCTTCAGGACAAGGGTGGATATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACGTGAGTGCCCATCTCTGCTATTTGTTGATGTTGCAAGAAAAAGATCATGAACTTAAAAGCTTTTCCTCTTCTGGCAGTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTAGGCTGGAATGAAGTGAACTGTTCAGTATTTCTTCACCTCAAAACCTCTCTTCGTTTTTCTGGCTTTGTTCATTGTCGCTGATTGCTTGTTTTTTTTTTAAATCCAGGTTTCGACAATGGCGCCGACAACTCCCCTCCATCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCGACTCTCCTCCGGCACCTTCTACCCCTAAAACAAGCAACAGCACGCAGTCATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGA

mRNA sequence

TCCTCCGGTCCATGTTACCCTCTCGTCCTCCTGATCCCATTTAAATTCTTCTCCTCAAATCCTCTTCACACCGGAAAACTTTTGGATCCTCTAATCGGCGTCCATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCCGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCAGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGATAACCACACCTACCGATACGGCGGTTTGGGAGATTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCGTACTTCCGTGGGCACTTCAGATGGGGAAGATTTAACGTTGAATCATTACAGTCCAAATGCTTCAACCACGAGCAAAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGGTGTGGTAAGGTACAAACTGGTATATTTTCCAGTATCGCAATTAATGGTCTTCTTGGGCTTGGCATGGGGAAGATATCTGTTCCATCCCTCTTAGCAAGCCAAGAGCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCGCTCAATCCCCACTCTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACCACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTTTCGTTACTCAGAATATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTCGAGTACTGCTACCAACTTCCTCTACGAACACTCTTTCAACAACCGCATCTGAATTTTACTATGGATAGTGGCTATATTTTTGGCGTTATTTCTATGTTTGTTGCCATTCCTACCGATGACAAGGGTGGATATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTAGGCTGGAATGAAGTGAACTGTTTCGACAATGGCGCCGACAACTCCCCTCCATCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCGACTCTCCTCCGGCACCTTCTACCCCTAAAACAAGCAACAGCACGCAGTCATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGA

Coding sequence (CDS)

ATGGCGTCGACATTCACTTCCGCCGCCCAAATGCTACTGTTTCTTTCTATTTTCTTTCTCGCCGGCGAGGTGGTTTCGCTCAAGTTCAATATCCACCGTCGGTTTTCGGATTCGATTAAGGGGATTCTAAACTCCGATGGTTTACTGGAGAAACACACCCCTGGATATTACGCAGCTATGGTCCACCGCGATCGACTACTTCACGCCCGGCGATTGGCCACCTCTAAAAGTGAAACGCCGCTGACGTTCTCTTACGATAACCACACCTACCGATACGGCGGTTTGGGAGATTTCTACTACGCCAATATATCTGTGGGAACGCCGGAGTTAGGTTTCTTAGTGGCATTGGACACTGGAAGCGATTTGTCCTGGTTACCTTGTGAATGCAGCAAATGCCGTACTTCCGTGGGCACTTCAGATGGGGAAGATTTAACGTTGAATCATTACAGTCCAAATGCTTCAACCACGAGCAAAAGTGTAGCCTGCAGCAACTCTTTGTGCGAGCTTGCAGACCAATGCCCTTCAAACAAAAGTTCTTGTCCTTACAAGATTCATGATGCGTCTCCCAATACATCATCTTCTGGGTATTTGGTAGAAGATATCTTGCACTTGGCCACCAATGATTCACAATCCAAACCCGTTGATGCCAAGATTACTTTTGGGTGTGGTAAGGTACAAACTGGTATATTTTCCAGTATCGCAATTAATGGTCTTCTTGGGCTTGGCATGGGGAAGATATCTGTTCCATCCCTCTTAGCAAGCCAAGAGCTCACCCCAGATTCATTCTCCATGTGTTTTGGATATGATGGTTATGGGAACATCGATTTTGGAGACATAGGCCCATCAGACCAGAGAGAAACATCGCTCAATCCCCACTCTTCATCCTACAATGTCACCATCATTCAGATAACTGTGGCAGGAAAACCCAACAATGTTGAATTTACCACAATTTTTGACTCTGGTTTCTCTTATACGTACCTCTCTGACCCAATTTACACTTTCGTTACTCAGAATATAGATGCAGCCATGGAATTAGACCGCGCTATATTTGATTCTGATTACCCATTCGAGTACTGCTACCAACTTCCTCTACGAACACTCTTTCAACAACCGCATCTGAATTTTACTATGGATAGTGGCTATATTTTTGGCGTTATTTCTATGTTTGTTGCCATTCCTACCGATGACAAGGGTGGATATGCCATTTGTCTTACCATTGCCAAGAGCACTGATATTAATCTCTTCGGACTGAATTTCATGGCTGGTCATCGTATCGTCTTCAATCGTGAAAAGATGGTTCTAGGCTGGAATGAAGTGAACTGTTTCGACAATGGCGCCGACAACTCCCCTCCATCCAACTCTCCGCCGGCCGACGCACCATCACCGTCCGGCGACTCTCCTCCGGCACCTTCTACCCCTAAAACAAGCAACAGCACGCAGTCATCGCCGGGAATTGGGACGGGTGATGCGACGCAGTTAAACCCGCTTGCCTGTCTCTCTGTTGTCCTTCTTGCTATTTTGTCTGTTGTTTGA

Protein sequence

MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLGLGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQSSPGIGTGDATQLNPLACLSVVLLAILSVV
Homology
BLAST of CmoCh03G006860 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 2.3e-109
Identity = 221/488 (45.29%), Postives = 303/488 (62.09%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNH 88
           F  H RFSD + G+L  DGL  + +  YY  M HRDRL+  RRLA ++ ++ +TFS  N 
Sbjct: 35  FEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAHRDRLIRGRRLA-NEDQSLVTFSDGNE 94

Query: 89  TYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNH 148
           T R   LG  +YAN++VGTP   F+VALDTGSDL WLPC+C+ C   +    G  L LN 
Sbjct: 95  TVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAPGGSSLDLNI 154

Query: 149 YSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATND 208
           YSPNAS+TS  V C+++LC   D+C S +S CPY+I   S  TSS+G LVED+LHL +ND
Sbjct: 155 YSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSND 214

Query: 209 SQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQELTPDSFSMCFG 268
             SK + A++TFGCG+VQTG+F    A NGL GLG+  ISVPS+LA + +  +SFSMCFG
Sbjct: 215 KSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG 274

Query: 269 YDGYGNIDFGDIGPSDQRETSLN---PHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSY 328
            DG G I FGD G  DQRET LN   PH  +YN+T+ +I+V G   ++EF  +FDSG S+
Sbjct: 275 NDGAGRISFGDKGSVDQRETPLNIRQPH-PTYNITVTKISVGGNTGDLEFDAVFDSGTSF 334

Query: 329 TYLSDPIYTFVTQNIDA-AMELDRAIFDSDYPFEYCYQL-PLRTLFQQPHLNFTMDSGYI 388
           TYL+D  YT ++++ ++ A++      DS+ PFEYCY L P +  FQ P +N TM  G  
Sbjct: 335 TYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGSS 394

Query: 389 FGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCF 448
           + V    V IP  D   Y  CL I K  DI++ G NFM G+R+VF+REK++LGW E +C+
Sbjct: 395 YPVYHPLVVIPMKDTDVY--CLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDCY 454

Query: 449 DNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQSSPGIGTGDATQLNPLACLSVV 508
                    + + P++  S S   P +   P+ +N     P   T  A     ++ LS+ 
Sbjct: 455 ----TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSAAYSLSIS-LSLF 513

Query: 509 LLAILSVV 511
             +IL+++
Sbjct: 515 FFSILAIL 513

BLAST of CmoCh03G006860 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.8e-61
Identity = 183/508 (36.02%), Postives = 255/508 (50.20%), Query Frame = 0

Query: 7   SAAQMLLFLSIFFLAGEVVSLKFN--IHRRFSD----SIKGILNSDGLLEKHTPGYYAAM 66
           S +  LLF  +F    E ++  F+  +  RFSD    SIK   +SD L  K +  YY  +
Sbjct: 3   SRSAFLLFCVLFLATEETLASLFSSRLIHRFSDEGRASIKTPSSSDSLPNKQSLEYYRLL 62

Query: 67  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGG-LGDFYYANISVGTPELGFLVALDTG 126
              D     +R+        L  S  + T   G   G  +Y  I +GTP + FLVALDTG
Sbjct: 63  AESD--FRRQRMNLGAKVQSLVPSEGSKTISSGNDFGWLHYTWIDIGTPSVSFLVALDTG 122

Query: 127 SDLSWLPCECSKCR--TSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNK 186
           S+L W+PC C +C   TS   S      LN Y+P++S+TSK   CS+ LC+ A  C S K
Sbjct: 123 SNLLWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSSTSKVFLCSHKLCDSASDCESPK 182

Query: 187 SSCPYKIHDASPNTSSSGYLVEDILHLATNDSQ-----SKPVDAKITFGCGKVQTGIF-S 246
             CPY ++  S NTSSSG LVEDILHL  N +      S  V A++  GCGK Q+G +  
Sbjct: 183 EQCPYTVNYLSGNTSSSGLLVEDILHLTYNTNNRLMNGSSSVKARVVIGCGKKQSGDYLD 242

Query: 247 SIAINGLLGLGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETS-LN 306
            +A +GL+GLG  +ISVPS L+   L  +SFS+CF  +  G I FGD+GPS Q+ T  L 
Sbjct: 243 GVAPDGLMGLGPAEISVPSFLSKAGLMRNSFSLCFDEEDSGRIYFGDMGPSIQQSTPFLQ 302

Query: 307 PHSSSYNVTIIQITVAGKPNN----VEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELD 366
             ++ Y+  I+ +      N+      FTT  DSG S+TYL + IY  V   ID  +   
Sbjct: 303 LDNNKYSGYIVGVEACCIGNSCLKQTSFTTFIDSGQSFTYLPEEIYRKVALEIDRHINAT 362

Query: 367 RAIFDSDYPFEYCYQLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTI 426
              F+    +EYCY+           L F+ ++ +   VI   + +    +G    CL I
Sbjct: 363 SKNFEG-VSWEYCYESSAEPKVPAIKLKFSHNNTF---VIHKPLFVFQQSQGLVQFCLPI 422

Query: 427 AKS--TDINLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSP--------- 483
           + S    I   G N+M G+R+VF+RE M LGW+   C ++  +  PP  SP         
Sbjct: 423 SPSGQEGIGSIGQNYMRGYRMVFDRENMKLGWSPSKCQEDKIE--PPQASPGSTSSPNPL 482

BLAST of CmoCh03G006860 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 7.0e-26
Identity = 117/470 (24.89%), Postives = 194/470 (41.28%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVS--LKFNIHRRFSDSIKGILNSDGLLEKHTPGYYA 60
           M +T   +    +   +F L  +VVS    FN+  +F+   K +      L+ H      
Sbjct: 1   MVTTMDPSRISRIVAVVFVLVIQVVSGNFVFNVTHKFAGKEKQLSE----LKSH------ 60

Query: 61  AMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDT 120
                D   HAR LA       L    D+     G     Y+  I +G+P   + V +DT
Sbjct: 61  -----DSFRHARMLA----NIDLPLGGDSRADSIG----LYFTKIKLGSPPKEYYVQVDT 120

Query: 121 GSDLSWLPC-ECSKC--RTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPS 180
           GSD+ W+ C  C KC  +T +G      + L+ Y    S+TSK+V C +  C    Q  +
Sbjct: 121 GSDILWVNCAPCPKCPVKTDLG------IPLSLYDSKTSSTSKNVGCEDDFCSFIMQSET 180

Query: 181 --NKSSCPYKIHDASPNTSSSGYLVEDI-LHLATNDSQSKPVDAKITFGCGKVQTGIF-- 240
              K  C Y +     +TS   ++ ++I L   T + ++ P+  ++ FGCGK Q+G    
Sbjct: 181 CGAKKPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQ 240

Query: 241 SSIAINGLLGLGMGKISVPSLLASQELTPDSFSMCF-GYDGYGNIDFGDIGPSDQRETSL 300
           +  A++G++G G    S+ S LA+   T   FS C    +G G    G++     + T +
Sbjct: 241 TDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFAVGEVESPVVKTTPI 300

Query: 301 NPHSSSYNVTIIQITVAGKP---------NNVEFTTIFDSGFSYTYLSDPIYTFVTQNID 360
            P+   YNV +  + V G P          N +  TI DSG +  YL   +Y  + + I 
Sbjct: 301 VPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKIT 360

Query: 361 AAMELDRAIFDSDYPFEYCYQLPLRTLFQQPHLNFTMDSG---------YIFGVISMFVA 420
           A  ++   +    +    C+     T    P +N   +           Y+F +      
Sbjct: 361 AKQQVKLHMVQETFA---CFSFTSNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLREDMYC 420

Query: 421 IPTDDKGGYAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNC 442
                 G     +T     D+ L G   ++   +V++ E  V+GW + NC
Sbjct: 421 FGWQSGG-----MTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADHNC 433

BLAST of CmoCh03G006860 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 1.3e-24
Identity = 96/361 (26.59%), Postives = 161/361 (44.60%), Query Frame = 0

Query: 99  YYANISVGTPELGFLVALDTGSDLSWLPCE-CSKCRTSVGTSDGEDLTLNHYSPNASTTS 158
           Y+  I +G+P   + V +DTGSD+ W+ C+ C KC T        +  L+ +  NAS+TS
Sbjct: 74  YFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNL----NFRLSLFDMNASSTS 133

Query: 159 KSVACSNSLCELADQCPSNKSS--CPYKIHDASPNTSSSGYLVEDILHL--ATNDSQSKP 218
           K V C +  C    Q  S + +  C Y I  A  +T S G  + D+L L   T D ++ P
Sbjct: 134 KKVGCDDDFCSFISQSDSCQPALGCSYHIVYADEST-SDGKFIRDMLTLEQVTGDLKTGP 193

Query: 219 VDAKITFGCGKVQTGIFSS--IAINGLLGLGMGKISVPSLLASQELTPDSFSMCF-GYDG 278
           +  ++ FGCG  Q+G   +   A++G++G G    SV S LA+       FS C     G
Sbjct: 194 LGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNVKG 253

Query: 279 YGNIDFGDIGPSDQRETSLNPHSSSYNVTIIQITVAGK----PNNV--EFTTIFDSGFSY 338
            G    G +     + T + P+   YNV ++ + V G     P ++     TI DSG + 
Sbjct: 254 GGIFAVGVVDSPKVKTTPMVPNQMHYNVMLMGMDVDGTSLDLPRSIVRNGGTIVDSGTTL 313

Query: 339 TYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQLPLRTLFQQPHLNFTMDSGYIFG 398
            Y    +Y  + + I A   +   I +  +    C+          P ++F  +      
Sbjct: 314 AYFPKVLYDSLIETILARQPVKLHIVEETF---QCFSFSTNVDEAFPPVSFEFEDSVKLT 373

Query: 399 VISMFVAIPTDDK----GGYAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVN 442
           V         +++    G  A  LT  + +++ L G   ++   +V++ +  V+GW + N
Sbjct: 374 VYPHDYLFTLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHN 426

BLAST of CmoCh03G006860 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 6.7e-21
Identity = 105/382 (27.49%), Postives = 170/382 (44.50%), Query Frame = 0

Query: 99  YYANISVGTPELG--FLVALDTGSDLSWLPCE--CSKCRTSVGTSDGEDLTLNHYSPNAS 158
           YY  I VG PE G  + + +DTGS+L+W+ C+  C+ C      + G +        N  
Sbjct: 203 YYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPCTSC------AKGANQLYKPRKDNLV 262

Query: 159 TTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPV 218
            +S++        +L + C  N   C Y+I  A  ++ S G L +D  HL  ++      
Sbjct: 263 RSSEAFCVEVQRNQLTEHC-ENCHQCDYEIEYAD-HSYSMGVLTKDKFHLKLHNGSL--A 322

Query: 219 DAKITFGCGKVQTGIF--SSIAINGLLGLGMGKISVPSLLASQELTPDSFSMCFGYD--G 278
           ++ I FGCG  Q G+   + +  +G+LGL   KIS+PS LAS+ +  +    C   D  G
Sbjct: 323 ESDIVFGCGYDQQGLLLNTLLKTDGILGLSRAKISLPSQLASRGIISNVVGHCLASDLNG 382

Query: 279 YGNIDFG-DIGPSDQRETSLNPHSSSYNVTIIQIT----------VAGKPNNVEFTTIFD 338
            G I  G D+ PS         H S  +   +Q+T          + G+   V    +FD
Sbjct: 383 EGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQGMLSLDGENGRVG-KVLFD 442

Query: 339 SGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQ----LPLRTL-----FQQ 398
           +G SYTY  +  Y+ +  ++     L+    DSD     C++     P  +L     F +
Sbjct: 443 TGSSYTYFPNQAYSQLVTSLQEVSGLELTRDDSDETLPICWRAKTNFPFSSLSDVKKFFR 502

Query: 399 PHLNFTMDSGYIFGVISMFVAIPTDD------KGGYAICLTIAKSTDIN-----LFGLNF 442
           P    T+  G  + +IS  + I  +D      KG   +CL I   + ++     + G   
Sbjct: 503 P---ITLQIGSKWLIISRKLLIQPEDYLIISNKGN--VCLGILDGSSVHDGSTIILGDIS 562

BLAST of CmoCh03G006860 vs. ExPASy TrEMBL
Match: A0A6J1GFR6 (aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453524 PE=3 SV=1)

HSP 1 Score: 1013.8 bits (2620), Expect = 2.5e-292
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           SSPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmoCh03G006860 vs. ExPASy TrEMBL
Match: A0A6J1IJH6 (aspartyl protease family protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478000 PE=3 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.2e-284
Identity = 496/510 (97.25%), Postives = 502/510 (98.43%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYT VTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QL L T FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmoCh03G006860 vs. ExPASy TrEMBL
Match: A0A6J1BTF7 (aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111005407 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 4.3e-180
Identity = 346/547 (63.25%), Postives = 400/547 (73.13%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEV-----VSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S AQMLLFLS+F LAGE+      S KF+IH RFSDSIKGIL+S+GL EK +PG
Sbjct: 1   MAWTFSSGAQMLLFLSVFLLAGELRSGEAGSFKFSIHHRFSDSIKGILDSEGLPEKQSPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYA MVHRDRL+H RRLAT+  + PLTF Y N T+  G LG  YYANISVGTPEL FLVA
Sbjct: 61  YYATMVHRDRLVHGRRLATTNGDPPLTFVYGNDTFLIGNLGFLYYANISVGTPELSFLVA 120

Query: 121 LDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPS 180
           LDTGSDL WLPCECS C T + T++G    LNHYSP  STTS SV CSNSLCELA+QC S
Sbjct: 121 LDTGSDLFWLPCECSSCLTYLNTTNGGKFGLNHYSPKDSTTSTSVPCSNSLCELANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFS-SIA 240
             S+CPY+I+  S NTSSSGYLV+D+LHLAT+D Q KPVDAKITFGCGK+QTGIF+ S A
Sbjct: 181 TTSTCPYEINYLSANTSSSGYLVQDVLHLATDDKQLKPVDAKITFGCGKIQTGIFADSAA 240

Query: 241 INGLLGLGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNP--H 300
            NGL+GLGM KISVPS LASQ LT DSFSMCFGYDG G IDFGDIG S QRET  N   +
Sbjct: 241 PNGLIGLGMEKISVPSFLASQGLTSDSFSMCFGYDGQGRIDFGDIGTSGQRETPFNTMVN 300

Query: 301 SSSYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDS 360
             SYNVT  QI V GK NN++F+ IFDSG S++Y++DP+Y+ + + +DA M+L+R  FD 
Sbjct: 301 FPSYNVTFTQIIVGGKSNNLQFSAIFDSGTSFSYITDPVYSLIAEQMDAGMKLERVKFDP 360

Query: 361 DYPFEYCYQLPLR-TLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTD 420
           D+PFEYCYQLP     F  P LNFTM  G  +G +  FV +PTD+ GG A CL I KSTD
Sbjct: 361 DFPFEYCYQLPPNANSFSHPRLNFTMKGGDNYGTLDPFVVVPTDNSGGLAACLGIVKSTD 420

Query: 421 -INLFGLNFMAGHRIVFNREKMVLGWNEVNCFDNG----------ADNSPPSNSPPADA- 480
            I+L G NFM G+RI+FNREKMVLGW E +C+DNG          ADNSPPS+SPP D+ 
Sbjct: 421 PIDLIGQNFMTGYRIIFNREKMVLGWTESDCYDNGAATPSDNSPPADNSPPSDSPPTDSS 480

Query: 481 -------PS---------PSGDSPPAPSTPKTSNSTQSSPGIGTGDATQLNPLACLSVVL 511
                  PS         PSGDSPPAPST   SN TQ  P IG GDAT+LNPL C+ V +
Sbjct: 481 PPTDSSPPSDSPQSGDSPPSGDSPPAPSTAGGSNGTQPLPRIGAGDATRLNPLGCVFVAI 540

BLAST of CmoCh03G006860 vs. ExPASy TrEMBL
Match: A0A0A0KGS0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507240 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 8.2e-179
Identity = 333/516 (64.53%), Postives = 388/516 (75.19%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLA-----GEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S  QMLL LS+FFLA     G   S KF IH RFSDSIK I  S+GL EKHTPG
Sbjct: 1   MAWTFSSGDQMLLVLSVFFLAGGLRSGHAASFKFTIHHRFSDSIKEIFGSEGLPEKHTPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYAAMVHRDRLLH R LAT+  +TPL FSY N TY   GLG+ YYAN+S+GTP L FLVA
Sbjct: 61  YYAAMVHRDRLLHGRNLATTNGDTPLMFSYGNETYELSGLGNLYYANVSIGTPGLYFLVA 120

Query: 121 LDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPS 180
           LDTGSDL WLPCEC+KC T +   D     LNHYS NAS+TS  V CS+SLCELA+QC S
Sbjct: 121 LDTGSDLFWLPCECTKCPTYLTKRDNGKFWLNHYSSNASSTSIRVPCSSSLCELANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSI-A 240
           NKSSCPY+ H  S N+SS+GYLV+DILH+AT+DSQ KPVD K+T GCGKVQTG FS++ A
Sbjct: 181 NKSSCPYQTHYLSENSSSAGYLVQDILHMATDDSQLKPVDVKVTLGCGKVQTGKFSNVTA 240

Query: 241 INGLLGLGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSS 300
            NGL+GLGMGK+SVPS LASQ LT DSFSMCFGY GYG IDFGDIGP  QRET  NP S 
Sbjct: 241 PNGLIGLGMGKVSVPSFLASQGLTTDSFSMCFGYYGYGRIDFGDIGPVGQRETPFNPASL 300

Query: 301 SYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDY 360
           SYNVTI+QI V  +P NV  T I DSG S+TYL+DP Y+ +T+N+DAAMEL+R   DSD+
Sbjct: 301 SYNVTILQIIVTNRPTNVHLTAIIDSGASFTYLTDPFYSIITENMDAAMELERIKSDSDF 360

Query: 361 PFEYCYQLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINL 420
           PFEYCY+L L T+FQQP+LNFTM+ G  F VI+ +V++ TDD  G A+CL I KSTDIN+
Sbjct: 361 PFEYCYRLSLATIFQQPNLNFTMEGGRKFDVITSYVSVDTDD--GPALCLAIVKSTDINV 420

Query: 421 FGLNFMAGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPK 480
            G NF  G+R+VFNREKM LGW EV+C      +S  +N+   D+P PSGDS P  STP+
Sbjct: 421 IGHNFFGGYRVVFNREKMTLGWKEVDC------DSYDANTSSDDSPPPSGDSSPTTSTPR 480

Query: 481 TSNSTQSSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SNSTQ SP IG GDA  LNP+  L VV+L IL V+
Sbjct: 481 KSNSTQPSPEIGAGDAMLLNPIVSLCVVILVILYVI 508

BLAST of CmoCh03G006860 vs. ExPASy TrEMBL
Match: A0A1S3B2V8 (aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 2.2e-176
Identity = 330/517 (63.83%), Postives = 388/517 (75.05%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLA-----GEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPG 60
           MA TF+S  QMLL LS+FFLA     G   S KF IH RFSDSIK I  S+GL EKHTPG
Sbjct: 1   MAWTFSSGDQMLLVLSVFFLAGGLRSGHAASFKFTIHHRFSDSIKEIFGSEGLAEKHTPG 60

Query: 61  YYAAMVHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVA 120
           YYAAMVHRDRLLH R LA +  +TPL FSY N TYR  GLG+ YYAN+S+GTP L FLVA
Sbjct: 61  YYAAMVHRDRLLHGRNLANTNGDTPLMFSYGNETYRISGLGNLYYANVSIGTPGLYFLVA 120

Query: 121 LDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPS 180
           LDTGS+L WLPCEC+KCRT + T D E   LNHYS NAS+TS  V CS+SLCE A+QC S
Sbjct: 121 LDTGSNLFWLPCECTKCRTYLTTRDNEKFWLNHYSSNASSTSIRVPCSSSLCEHANQCSS 180

Query: 181 NKSSCPYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSI-A 240
           N+SSCPYK H  S N+SS+GYLV+DILH+AT+DSQ KPVDAK+T GCGKVQTG F++  A
Sbjct: 181 NRSSCPYKTHYPSQNSSSAGYLVQDILHMATDDSQLKPVDAKVTLGCGKVQTGKFANFTA 240

Query: 241 INGLLGLGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSS 300
            NGL+GLGMGK+SVPS LASQ LT DSFSMCFGY G G IDFGDIG   QRET LNP S 
Sbjct: 241 ANGLIGLGMGKLSVPSFLASQGLTADSFSMCFGYYGNGRIDFGDIGSVGQRETPLNPTSL 300

Query: 301 SYNVTIIQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDY 360
           SYNVTI+QI V  +P NV FT I D+G S+TYL+DP Y+ ++  +D A+EL+R   DSD+
Sbjct: 301 SYNVTILQIIVTNRPTNVHFTAIIDTGSSFTYLTDPFYSIISDKMDEAIELERIASDSDF 360

Query: 361 PFEYCYQLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINL 420
           PFEYCYQL + T+F QP +NFTM+ G  F VIS +V I TDD  G A+CL I KSTDIN+
Sbjct: 361 PFEYCYQLSMGTIFLQPTMNFTMEGGRKFDVISSYVMINTDD--GIALCLAIVKSTDINV 420

Query: 421 FGLNFMAGHRIVFNREKMVLGWNE-VNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTP 480
            G N ++G+R+VFNREKM LGW E V+C + GAD      +P  ++P P G SPP  S+P
Sbjct: 421 IGSNLLSGYRVVFNREKMTLGWKEVVDCDNYGAD------TPSGESPPPFGGSPPPTSSP 480

Query: 481 KTSNSTQSSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           + SNSTQ SP IGTGDA +LNP+  L VV+L ILSVV
Sbjct: 481 RKSNSTQPSPEIGTGDAMRLNPIVSLCVVILVILSVV 509

BLAST of CmoCh03G006860 vs. NCBI nr
Match: XP_022950419.1 (aspartyl protease family protein 1-like [Cucurbita moschata])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.1e-292
Identity = 510/510 (100.00%), Postives = 510/510 (100.00%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           SSPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmoCh03G006860 vs. NCBI nr
Match: XP_022977812.1 (aspartyl protease family protein 1-like [Cucurbita maxima])

HSP 1 Score: 986.5 bits (2549), Expect = 8.7e-284
Identity = 496/510 (97.25%), Postives = 502/510 (98.43%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKC TSVGTSDGED+TLNHYSPNASTTS+SVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCHTSVGTSDGEDITLNHYSPNASTTSRSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQ+LTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQDLTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYT VTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTLVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QL L T FQQPHLNFTMDSGYIFGV+SMFVAIPTDDKGG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 QLSLGTHFQQPHLNFTMDSGYIFGVVSMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSG SPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGHSPPAPSTTRTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
            SPGIGTGDATQLNPLACLSVVLLAILSVV
Sbjct: 481 LSPGIGTGDATQLNPLACLSVVLLAILSVV 510

BLAST of CmoCh03G006860 vs. NCBI nr
Match: XP_023521822.1 (aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 967.2 bits (2499), Expect = 5.4e-278
Identity = 486/510 (95.29%), Postives = 495/510 (97.06%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLH RRL TSKSETPLTFSYDNHTY YG LGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHGRRLVTSKSETPLTFSYDNHTYHYGSLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DL WLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLFWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLAT+DSQSKPVDAKITFGCGKVQTGIFS++A NGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATDDSQSKPVDAKITFGCGKVQTGIFSNVAANGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSL P+SSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLKPNSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKP+NVEFT IFDSG SYTYL+DPIYT VTQ IDAAMELDR IFDSDYPFEYCY
Sbjct: 301 IQITVAGKPSNVEFTAIFDSGSSYTYLADPIYTLVTQKIDAAMELDRFIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QLPL TLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 QLPLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTTRTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           SSPGIGTGDATQLNPLACLSVVLLAIL +V
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILFIV 510

BLAST of CmoCh03G006860 vs. NCBI nr
Match: XP_023544616.1 (aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.4 bits (2336), Expect = 4.3e-259
Identity = 464/510 (90.98%), Postives = 477/510 (93.53%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLH RRL TSKSETPLTFSYDNHTY YG LGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHGRRLVTSKSETPLTFSYDNHTYHYGSLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DL WLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLFWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLAT+DSQSKPVDAKITFGCGKVQTGIFS++A NGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATDDSQSKPVDAKITFGCGKVQTGIFSNVAANGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSL P+SSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLKPNSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKP+NVEFT IFDSG SYTYL+DPIYT VTQ +   +    +  DSD    + +
Sbjct: 301 IQITVAGKPSNVEFTAIFDSGSSYTYLADPIYTLVTQKVSYYLGNKHSRQDSD---SHLF 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
              L TLFQQPHLNFTMDSGYIFGVISMFVAIPTD  GG+AICLTIAKSTDINLFGLNFM
Sbjct: 361 LSSLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTD--GGHAICLTIAKSTDINLFGLNFM 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
           AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPST +TSNSTQ
Sbjct: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTTRTSNSTQ 480

Query: 481 SSPGIGTGDATQLNPLACLSVVLLAILSVV 511
           SSPGIGTGDATQLNPLACLSVVLLAIL VV
Sbjct: 481 SSPGIGTGDATQLNPLACLSVVLLAILFVV 505

BLAST of CmoCh03G006860 vs. NCBI nr
Match: KAG6603890.1 (Peroxidase 64, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902.9 bits (2332), Expect = 1.3e-258
Identity = 460/487 (94.46%), Postives = 461/487 (94.66%), Query Frame = 0

Query: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60
           MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM
Sbjct: 1   MASTFTSAAQMLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTPGYYAAM 60

Query: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120
           VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS
Sbjct: 61  VHRDRLLHARRLATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGS 120

Query: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180
           DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC
Sbjct: 121 DLSWLPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSC 180

Query: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240
           PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG
Sbjct: 181 PYKIHDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAINGLLG 240

Query: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300
           LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI
Sbjct: 241 LGMGKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRETSLNPHSSSYNVTI 300

Query: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360
           IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY
Sbjct: 301 IQITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCY 360

Query: 361 QLPLRTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 420
           QLPL TLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFG    
Sbjct: 361 QLPLGTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGR--- 420

Query: 421 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQ 480
                                FDNGADNSPPSNSPPADAPSPSGDSPPAPSTP+TSNSTQ
Sbjct: 421 ---------------------FDNGADNSPPSNSPPADAPSPSGDSPPAPSTPRTSNSTQ 463

Query: 481 SSPGIGT 488
           SSPGIGT
Sbjct: 481 SSPGIGT 463

BLAST of CmoCh03G006860 vs. TAIR 10
Match: AT2G17760.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 397.5 bits (1020), Expect = 1.6e-110
Identity = 221/488 (45.29%), Postives = 303/488 (62.09%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLLEKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDNH 88
           F  H RFSD + G+L  DGL  + +  YY  M HRDRL+  RRLA ++ ++ +TFS  N 
Sbjct: 35  FEFHHRFSDQVVGVLPGDGLPNRDSSKYYRVMAHRDRLIRGRRLA-NEDQSLVTFSDGNE 94

Query: 89  TYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSKCRTSVGTSDGEDLTLNH 148
           T R   LG  +YAN++VGTP   F+VALDTGSDL WLPC+C+ C   +    G  L LN 
Sbjct: 95  TVRVDALGFLHYANVTVGTPSDWFMVALDTGSDLFWLPCDCTNCVRELKAPGGSSLDLNI 154

Query: 149 YSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLATND 208
           YSPNAS+TS  V C+++LC   D+C S +S CPY+I   S  TSS+G LVED+LHL +ND
Sbjct: 155 YSPNASSTSTKVPCNSTLCTRGDRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHLVSND 214

Query: 209 SQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQELTPDSFSMCFG 268
             SK + A++TFGCG+VQTG+F    A NGL GLG+  ISVPS+LA + +  +SFSMCFG
Sbjct: 215 KSSKAIPARVTFGCGQVQTGVFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG 274

Query: 269 YDGYGNIDFGDIGPSDQRETSLN---PHSSSYNVTIIQITVAGKPNNVEFTTIFDSGFSY 328
            DG G I FGD G  DQRET LN   PH  +YN+T+ +I+V G   ++EF  +FDSG S+
Sbjct: 275 NDGAGRISFGDKGSVDQRETPLNIRQPH-PTYNITVTKISVGGNTGDLEFDAVFDSGTSF 334

Query: 329 TYLSDPIYTFVTQNIDA-AMELDRAIFDSDYPFEYCYQL-PLRTLFQQPHLNFTMDSGYI 388
           TYL+D  YT ++++ ++ A++      DS+ PFEYCY L P +  FQ P +N TM  G  
Sbjct: 335 TYLTDAAYTLISESFNSLALDKRYQTTDSELPFEYCYALSPNKDSFQYPAVNLTMKGGSS 394

Query: 389 FGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFMAGHRIVFNREKMVLGWNEVNCF 448
           + V    V IP  D   Y  CL I K  DI++ G NFM G+R+VF+REK++LGW E +C+
Sbjct: 395 YPVYHPLVVIPMKDTDVY--CLAIMKIEDISIIGQNFMTGYRVVFDREKLILGWKESDCY 454

Query: 449 DNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNSTQSSPGIGTGDATQLNPLACLSVV 508
                    + + P++  S S   P +   P+ +N     P   T  A     ++ LS+ 
Sbjct: 455 ----TGETSARTLPSNRSSSSARPPASSFDPEATNIPSQRPNTSTTSAAYSLSIS-LSLF 513

Query: 509 LLAILSVV 511
             +IL+++
Sbjct: 515 FFSILAIL 513

BLAST of CmoCh03G006860 vs. TAIR 10
Match: AT4G35880.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.2e-97
Identity = 207/491 (42.16%), Postives = 291/491 (59.27%), Query Frame = 0

Query: 11  MLLFLSIFFLAGEVVSLKFNIHRRFSDSIKGILNSDGLLEKHTP----GYYAAMVHRDRL 70
           +L+ LS     G + +  F +H RFSD +K   +S G   K  P     Y+ A+V RD L
Sbjct: 15  ILMLLSFGSCNGRIFT--FEMHHRFSDEVKQWSDSTGRFAKFPPKGSFEYFNALVLRDWL 74

Query: 71  LHARRL--ATSKSETPLTFSYDNHTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSW 130
           +  RRL  + S+SE+ LTFS  N T R   LG  +Y  + +GTP + F+VALDTGSDL W
Sbjct: 75  IRGRRLSESESESESSLTFSDGNSTSRISSLGFLHYTTVKLGTPGMRFMVALDTGSDLFW 134

Query: 131 LPCECSKCRTSVGTSDGEDLTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKI 190
           +PC+C KC  + G +   +  L+ Y+P  STT+K V C+NSLC   +QC    S+CPY +
Sbjct: 135 VPCDCGKCAPTEGATYASEFELSIYNPKVSTTNKKVTCNNSLCAQRNQCLGTFSTCPYMV 194

Query: 191 HDASPNTSSSGYLVEDILHLATNDSQSKPVDAKITFGCGKVQTGIFSSIAI-NGLLGLGM 250
              S  TS+SG L+ED++HL T D   + V+A +TFGCG+VQ+G F  IA  NGL GLGM
Sbjct: 195 SYVSAQTSTSGILMEDVMHLTTEDKNPERVEAYVTFGCGQVQSGSFLDIAAPNGLFGLGM 254

Query: 251 GKISVPSLLASQELTPDSFSMCFGYDGYGNIDFGDIGPSDQRET--SLNPHSSSYNVTII 310
            KISVPS+LA + L  DSFSMCFG+DG G I FGD G SDQ ET  +LNP   +YN+T+ 
Sbjct: 255 EKISVPSVLAREGLVADSFSMCFGHDGVGRISFGDKGSSDQEETPFNLNPSHPNYNITVT 314

Query: 311 QITVAGKPNNVEFTTIFDSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQ 370
           ++ V     + EFT +FD+G S+TYL DP+YT V+++  +  +  R   DS  PFEYCY 
Sbjct: 315 RVRVGTTLIDDEFTALFDTGTSFTYLVDPMYTTVSESFHSQAQDKRHSPDSRIPFEYCYD 374

Query: 371 LPL-RTLFQQPHLNFTMDSGYIFGVISMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFM 430
           +         P L+ TM     F +    + I T+  G    CL I KS+++N+ G N+M
Sbjct: 375 MSNDANASLIPSLSLTMKGNSHFTINDPIIVISTE--GELVYCLAIVKSSELNIIGQNYM 434

Query: 431 AGHRIVFNREKMVLGWNEVNCFDNGADNSPPSNSPPADAPSPS--------GDSPPAPST 484
            G+R+VF+REK+VL W + +C+D    N+  + +    A +P+         +S     T
Sbjct: 435 TGYRVVFDREKLVLAWKKFDCYDIEETNTTVAGTNKTAAVAPAMAAGIKTHNNSSELHKT 494

BLAST of CmoCh03G006860 vs. TAIR 10
Match: AT3G51330.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 351.7 bits (901), Expect = 1.0e-96
Identity = 220/501 (43.91%), Postives = 293/501 (58.48%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSD +K  L  D L+ EK +  Y+  +  RDRL+  R LA++  ETP+TF   N
Sbjct: 31  FEVHHMFSDRVKQSLGLDDLVPEKGSLEYFKVLAQRDRLIRGRGLASNNEETPITFMRGN 90

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCEC-SKC---RTSVGTSDGED 148
            T     LG  +YAN+SVGTP   FLVALDTGSDL WLPC C S C      VG S    
Sbjct: 91  RTISIDLLGFLHYANVSVGTPATWFLVALDTGSDLFWLPCNCGSTCIRDLKEVGLSQSRP 150

Query: 149 LTLNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILH 208
           L L  YSPN S+TS S+ CS+  C  + +C S  SSCPY+I   S +T ++G L ED+LH
Sbjct: 151 LNL--YSPNTSSTSSSIRCSDDRCFGSSRCSSPASSCPYQIQYLSKDTFTTGTLFEDVLH 210

Query: 209 LATNDSQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQELTPDSF 268
           L T D   +PV A IT GCGK QTG   SS A+NGLLGLG+   SVPS+LA  ++T +SF
Sbjct: 211 LVTEDEGLEPVKANITLGCGKNQTGFLQSSAAVNGLLGLGLKDYSVPSILAKAKITANSF 270

Query: 269 SMCFG--YDGYGNIDFGDIGPSDQRETSLNP--HSSSYNVTIIQITVAGKPNNVEFTTIF 328
           SMCFG   D  G I FGD G +DQ ET L P   S +Y V++ +++V G    V+   +F
Sbjct: 271 SMCFGNIIDVVGRISFGDKGYTDQMETPLLPTEPSPTYAVSVTEVSVGGDAVGVQLLALF 330

Query: 329 DSGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQL-PLRTLFQQPHLNFTM 388
           D+G S+T+L +P Y  +T+  D  +   R   D + PFE+CY L P +T    P +  T 
Sbjct: 331 DTGTSFTHLLEPEYGLITKAFDDHVTDKRRPIDPELPFEFCYDLSPNKTTILFPRVAMTF 390

Query: 389 DSG-YIFGVISMFVAIPTDDKGGYAICLTIAKSTD--INLFGLNFMAGHRIVFNREKMVL 448
           + G  +F    +F+    D+   Y  CL I KS D  IN+ G NFM+G+RIVF+RE+M+L
Sbjct: 391 EGGSQMFLRNPLFIVWNEDNSAMY--CLGILKSVDFKINIIGQNFMSGYRIVFDRERMIL 450

Query: 449 GWNEVNCFDNGADNSPPSNSPPADAPSPSGDS-------PPAPSTPKTSNSTQSSPGIGT 508
           GW   +CF++ +  S     P  +APSPS  +       PPA +TP   +   S+   GT
Sbjct: 451 GWKRSDCFEDESLESTTPPPPETEAPSPSASTPLPSLLPPPAAATPPQIDPRNSTRNSGT 510

BLAST of CmoCh03G006860 vs. TAIR 10
Match: AT3G51350.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 339.3 bits (869), Expect = 5.2e-93
Identity = 213/499 (42.69%), Postives = 291/499 (58.32%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSDS+K  L    L+ E+ +  Y+  + HRDRL+  R LA++  ETP+TF   N
Sbjct: 31  FEVHHIFSDSVKQSLGLGDLVPEQGSLEYFKVLAHRDRLIRGRGLASNNDETPITFDGGN 90

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCECSK--CRTSVGTSDGEDLT 148
            T     LG  YYAN+SVGTP   FLVALDTGSDL WLPC C     R        + + 
Sbjct: 91  LTVSVKLLGSLYYANVSVGTPPSSFLVALDTGSDLFWLPCNCGTTCIRDLEDIGVPQSVP 150

Query: 149 LNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLA 208
           LN Y+PNASTTS S+ CS+  C  + +C S  S CPY+I   S +T + G L++D+LHLA
Sbjct: 151 LNLYTPNASTTSSSIRCSDKRCFGSKKCSSPSSICPYQI-SYSNSTGTKGTLLQDVLHLA 210

Query: 209 TNDSQSKPVDAKITFGCGKVQTGIFS-SIAINGLLGLGMGKISVPSLLASQELTPDSFSM 268
           T D    PV A +T GCG+ QTG+F  + ++NG+LGLG+   SVPSLLA   +T +SFSM
Sbjct: 211 TEDENLTPVKANVTLGCGQKQTGLFQRNNSVNGVLGLGIKGYSVPSLLAKANITANSFSM 270

Query: 269 CFG--YDGYGNIDFGDIGPSDQRET---SLNPHSSSYNVTIIQITVAGKPNNVEFTTIFD 328
           CFG      G I FGD G +DQ ET   S+ P S++Y V I  ++VAG P ++     FD
Sbjct: 271 CFGRVIGNVGRISFGDRGYTDQEETPFISVAP-STAYGVNISGVSVAGDPVDIRLFAKFD 330

Query: 329 SGFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQL-PLRTLFQQPHLNFTMD 388
           +G S+T+L +P Y  +T++ D  +E  R   D + PFE+CY L P  T  Q P +  T  
Sbjct: 331 TGSSFTHLREPAYGVLTKSFDELVEDRRRPVDPELPFEFCYDLSPNATTIQFPLVEMTFI 390

Query: 389 SGYIFGVISMFVAIPTDDKGGYAICLTIAKST--DINLFGLNFMAGHRIVFNREKMVLGW 448
            G    + + F    T + G    CL + KS    IN+ G NF+AG+RIVF+RE+M+LGW
Sbjct: 391 GGSKIILNNPFFTARTQE-GNVMYCLGVLKSVGLKINVIGQNFVAGYRIVFDRERMILGW 450

Query: 449 NEVNCFDNGADNSPPSNSPPADAPSPSGDSPPAPSTPKTSNST-------QSSPGIGTGD 508
            +  CF++ +  S     P  +AP+PS  +PP  S P T ++T        S+   GTG 
Sbjct: 451 KQSLCFEDESLESTTPPPPEVEAPAPSVSAPPPRSLPPTVSATPPPINPRNSTGNPGTGG 510

BLAST of CmoCh03G006860 vs. TAIR 10
Match: AT3G51340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 335.9 bits (860), Expect = 5.7e-92
Identity = 212/506 (41.90%), Postives = 290/506 (57.31%), Query Frame = 0

Query: 29  FNIHRRFSDSIKGILNSDGLL-EKHTPGYYAAMVHRDRLLHARRLATSKSETPLTFSYDN 88
           F +H  FSD +K  L  D L+ E  +  Y+  + HRDR +  R LA++  ETPLT    N
Sbjct: 32  FEVHHMFSDVVKQTLGFDDLVPENGSLEYFKVLAHRDRFIRGRGLASNNEETPLTSIGSN 91

Query: 89  HTYRYGGLGDFYYANISVGTPELGFLVALDTGSDLSWLPCEC-SKCRTSVGTSD-GEDLT 148
            T     LG  +YAN+S+GTP   FLVALDTGSDL WLPC C + C   +  +   E + 
Sbjct: 92  LTLALNFLGFLHYANVSLGTPATWFLVALDTGSDLFWLPCNCGTTCIHDLKDARFSESVP 151

Query: 149 LNHYSPNASTTSKSVACSNSLCELADQCPSNKSSCPYKIHDASPNTSSSGYLVEDILHLA 208
           LN Y+PNASTTS S+ CS+  C  + +C S +S CPY+I   S NT ++G L++D+LHL 
Sbjct: 152 LNLYTPNASTTSSSIRCSDKRCFGSGKCSSPESICPYQI-ALSSNTVTTGTLLQDVLHLV 211

Query: 209 TNDSQSKPVDAKITFGCGKVQTGIF-SSIAINGLLGLGMGKISVPSLLASQELTPDSFSM 268
           T D   KPV+A +T GCG+ QTG F + IA+NG+LGL M + SVPSLLA   +T +SFSM
Sbjct: 212 TEDEDLKPVNANVTLGCGQNQTGAFQTDIAVNGVLGLSMKEYSVPSLLAKANITANSFSM 271

Query: 269 CFG--YDGYGNIDFGDIGPSDQRETSL--NPHSSSYNVTIIQITVAGKPNNVEFTTIFDS 328
           CFG      G I FGD G +DQ ET L     S++Y V +  ++V G P +V    +FD+
Sbjct: 272 CFGRIISVVGRISFGDKGYTDQEETPLVSLETSTAYGVNVTGVSVGGVPVDVPLFALFDT 331

Query: 329 GFSYTYLSDPIYTFVTQNIDAAMELDRAIFDSDYPFEYCYQLPLRTLFQQPHLNFTMDSG 388
           G S+T L +  Y   T+  D  ME  R   D D+PFE+CY L      ++ HLN      
Sbjct: 332 GSSFTLLLESAYGVFTKAFDDLMEDKRRPVDPDFPFEFCYDL------REEHLNSDARPR 391

Query: 389 YIFGVI--------------SMFVAIPTDDKGGYAICLTIAKSTDINLFGLNFMAGHRIV 448
           ++                      ++   ++G    CL I KS ++N+ G N M+GHRIV
Sbjct: 392 HMQSKCYNPCRDDFRWRIQNDSQESVSYSNEGTKMYCLGILKSINLNIIGQNLMSGHRIV 451

Query: 449 FNREKMVLGWNEVNCFDNG--ADNSPPSNSPPADAPSPSGDSPP--APSTPKTSNSTQSS 508
           F+RE+M+LGW + NCF++   A  SPP   P  +AP PS  +PP  A +TP T +   S+
Sbjct: 452 FDRERMILGWKQSNCFEDESLASESPP--PPEIEAPPPSVSTPPPAASATPPTIDPRNST 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYV92.3e-10945.29Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX201.8e-6136.02Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q4V3D27.0e-2624.89Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9S9K41.3e-2426.59Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q9M9A86.7e-2127.49Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GFR62.5e-292100.00aspartyl protease family protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1IJH64.2e-28497.25aspartyl protease family protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A6J1BTF74.3e-18063.25aspartyl protease family protein 1-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KGS08.2e-17964.53Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G50724... [more]
A0A1S3B2V82.2e-17663.83aspartyl protease family protein 1-like OS=Cucumis melo OX=3656 GN=LOC103485160 ... [more]
Match NameE-valueIdentityDescription
XP_022950419.15.1e-292100.00aspartyl protease family protein 1-like [Cucurbita moschata][more]
XP_022977812.18.7e-28497.25aspartyl protease family protein 1-like [Cucurbita maxima][more]
XP_023521822.15.4e-27895.29aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_023544616.14.3e-25990.98aspartyl protease family protein 1-like [Cucurbita pepo subsp. pepo][more]
KAG6603890.11.3e-25894.46Peroxidase 64, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G17760.11.6e-11045.29Eukaryotic aspartyl protease family protein [more]
AT4G35880.11.2e-9742.16Eukaryotic aspartyl protease family protein [more]
AT3G51330.11.0e-9643.91Eukaryotic aspartyl protease family protein [more]
AT3G51350.15.2e-9342.69Eukaryotic aspartyl protease family protein [more]
AT3G51340.15.7e-9241.90Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 72..278
e-value: 3.8E-40
score: 139.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 282..447
e-value: 2.1E-22
score: 81.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 96..442
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 99..278
e-value: 3.0E-35
score: 122.0
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 312..436
e-value: 4.8E-10
score: 39.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 470..487
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 453..469
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 445..487
NoneNo IPR availablePANTHERPTHR13683:SF826ASPARTYL PROTEASE FAMILY PROTEIN 1coord: 8..454
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 8..454
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 114..125
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 99..437
score: 31.361834

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G006860.1CmoCh03G006860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016020 membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity