CmoCh03G003620 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G003620
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionVacuolar cation/proton exchanger
LocationCmo_Chr03: 4609713 .. 4613688 (+)
RNA-Seq ExpressionCmoCh03G003620
SyntenyCmoCh03G003620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GACCAACAGCGCCAACTCTATCTTCCTCGTCCCTTTTTCCAGCCTCAAAAAAAGCGAACCCATGGCTTCCCAATCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGAGTGAGAACCCTTATCCCTCTCTATTTCCCGTGAAACGACATGTCGTTCTGGTTAATTTAATTAATTAACTAATTAATATATGTATTTAGCCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGTAAGCTTCTCTGACCTTTTTATTAATATCAAATATTTATTATTATTCTTTTATTTCCTCTTATTGTTTCCATTTGTCCTTGAAGGCAAATCGCTTATTTCACCGGCCCAACAGGTAATCGGTCGTTTATATTAATTTTTTTTATTTATTTATAAAATAAAATAGATTTAATTAATGATGAAATTAAAAATTTGGTATATAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTCACACAAGGAAAGATAGACGTGGTGAAGTTCTCTCTTTTGGGCTCTGTTCTTTCCAACCTTCTTCTTGTTCTTGGCACCTCTCTCTTCTGTGGTGGCATCATTAATAAAGAGCAGAGTTATGACAGAGTAAGTAACAGCTCTCTCTCTCGCATTATATTAATTTTTTAGATCAATTTTAACGTTAAGTAACCATCTAAGTCCACCGCTAACACATATTGTTATATTTGGTTTTTTTTTTTTTTTTCTGAGCTTACTCTCCGAGTTTTAAAACATGTTCTTGCGTGTGAGATCCCACGTTAGTTGGAGAAGGAAACAAAGAGTTTATAATTCTACCCTAACAGACGCGTTTTAAAACTTTAGAAAAAGCGTGGGAGACAAAGTCGAACCTAACCGACCATTTTAAAACTTTGGAGTAAGCCCAGAAGTGAAAATCTAAAAAAGACCATACGAGTTAGAGAGGAGAACAAATGGGGTGGAAACTTCTGTCTAACATACCTGTTTTAAAATATTGGGAGAAGTCGTGATGAAAAGCTTAAAGAAGATAATGTATCCAACGGTGTAGTTGAACTATTATAATAATATCATAGCCAAATAATAGACCCAATGAGTGGAGGGTGTGGAAATCTGTTACTACTGTTGGATGATGAAAGTCCCACATCGACTAATTTAGAGACTGATGATGAGTTTATAATTAAGGAATACTCTCTTCGTTGGTATGAGGTCTCTTGGGGAAGCCCAAAACAAGCCTGCGAGAGCTTAATATGGTATCAATTAAGGGAGATGCAGTTGTATGAGGAGAGGTTATAGAAATATAATTAAATATGTAATTTTGTAATTTTAACAGAGGCAAGCGGATGTGAACGCACTGATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACGGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGTACGTTCCTTCTCCTCTCTTTAATATTCTATTTTTTATTTAATAAAATTCAGTTAAAAAAAAAAAATTAAGTTTATATACACCCTTTCCATCCATTCATTATTATTATTATTATTATTTAATTTTCAAAATTAAATCAAGTAAAAAGTTAGTACATTTTTGAATATTTAGTTAAAAAATAAGAAAAATTGTGACAAATAATTAAAAAGTTATTACATTATAATTATAAGAAGGCGATGTATTTGTATCTGACTAGTGGCTAAGGATGTTTTGGTGGTTCTAAATGAACCACTACGACATGCTATGGTCCCGAATCCTCCACTAGAATAGTCGTTTATCTACTTTTAAATACTCAAATTTAGTTTCAAATTTTTAATAAATCGTAAAAATAGTCTCGAGTATAAATTTTAAAAAATAACAGTTTCTTCGATCGTTTCGCATTTTATAAAATTAAGTAAAAATAACTAATTTTAAGATTTTATTAAAATTACACACAATAATTATAATAATAATCAATGTCTTGGTGGGTTGATTTTTGAACCAAGATAAACATTTATTTATTAAAATTGAAATGTTTTTTTTTATTGTGTGAGAATTAAATTATTTAATTATATTATTGGAAAATTAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTGAACACAATTGAGGTAAATTTTAATTACTGTATATTTAAAGGGCTTTTCAGCAAAAAATGAAATATATTTTTTTATTTCTCTTTTAATAAATTTAAAATATAACCTAAAGTTTATTATGTTAATTTCAGGAAGCATCTACTACGTGGGGTTTGTCGGTCAGTTTCCTTAGCATAATCTTGCTCCCTATCGTCGGAAATGCAGCCGAACACGCCGGGGCTATCATATTCGCCTTCAAGAATAAGTTGGTAAGTATCGTAAATTAAAAATTCACGAATAGGTAATTCAATTAATTTTTAAAAAATTAAATTTATGGAGATTTATTAAATATAAAATTAAATATTTTATTTTGTTTGCAGGACATATCTTTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGGTACGTATGTGTAATTATTGAAAACTTCAAACCCCTCTTGGAAAAAGGTTGAAATTACACGAACTGACCCCTGAAGAATATGTAATTGCAGATTCCACTGTGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATCATCATTGCTGCTTGCTTCTTTGTTTCCAAGACCCAAGAAGGTGAGTGACTAAATTGCACGGTTCGGGTTCGGTTCGAGTGAGTTTAGTTCACGGGTTTCAATAGTCTATTTTCGTGTAGTTTTAGGGTTAAGAAGAGTTTGAATTGTGTGTAGCTTGTACCTAATTTGGAGAGGGGAGTGCCAGCGAGAACGCTAGGCCTCAAAGGGAGGTGGATTGTGAGATCCCACATCGGTTGGAGAAGAAAATGAAGCATTCTTTATAAGCGTGTGAAAATCTCTTCCTTGCATACGTGTTTCAAAAACTTTGATAGGAAGCCTAAAAGGGAAACCTCAAAGAGGACAATATCTACTGGGAGTGGGTTTTGGTTTAAAAACTTTGACAAAAGCGAAACCCTAAAGATGACAATATCTGCTACGGATGGGTTTGGGTTGTTACATGCGTGTATAGAGTACTTTTAATGAAATTAGGTCGTTAAATTAACGTATTTTTGTTTTTGGCCCATGCAGATGACAACAATGCCCCCAACTTGGGACTCCAAGCTTCAAACCAATCAATCTTCAGAGCTTAAAGGCTAATATGATGCTTTTAAGTGGGTCAAATTGCAAAATGAGAGAGGGGTTTCTTTTGAAATCTGGTCATAAAGAAGGACCAAACAGCAAAATCAAATGGATTTGTTATTAAATTCTTTGATATCAGAATTGGCCTGGATCGAACGCTTGCCGCCTGCCTGGTGGAACGCTTAG

mRNA sequence

GACCAACAGCGCCAACTCTATCTTCCTCGTCCCTTTTTCCAGCCTCAAAAAAAGCGAACCCATGGCTTCCCAATCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGACCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGCAAATCGCTTATTTCACCGGCCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTCACACAAGGAAAGATAGACGTGGTGAAGTTCTCTCTTTTGGGCTCTGTTCTTTCCAACCTTCTTCTTGTTCTTGGCACCTCTCTCTTCTGTGGTGGCATCATTAATAAAGAGCAGAGTTATGACAGAAGGCAAGCGGATGTGAACGCACTGATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACGGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTGAACACAATTGAGGAAGCATCTACTACGTGGGGTTTGTCGGTCAGTTTCCTTAGCATAATCTTGCTCCCTATCGTCGGAAATGCAGCCGAACACGCCGGGGCTATCATATTCGCCTTCAAGAATAAGTTGGACATATCTTTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGATTCCACTGTGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATCATCATTGCTGCTTGCTTCTTTGTTTCCAAGACCCAAGAAGAATTGGCCTGGATCGAACGCTTGCCGCCTGCCTGGTGGAACGCTTAG

Coding sequence (CDS)

ATGGCTTCCCAATCCGAGCCATGGCTCATGGAAAACGGAACCCTTAAGCGCTTAACCAAGGAGAGTCGCCACGGCCATGGCCGAACTGCCCACAACTTGTCCTCCTCTTCCCTACGCAAAAAGTCCGATTTAACCCTCGTCTCCAAAATCCAATTCGGCTGTCTCCGCAAGTTCCTCATCAATCTTCAGGAGGTCATTTTGGGAACTAAACTTTCCATTCTCTTCCCTGCCATTCCGCTCGCTATTGTCGCTCAGAGTTTCGGGTTTGGAAGACCGTGGATTTTTGCTTTGAGCTTGCTTGGCCTCACGCCATTGGCTGAACGTGTCAGCTTCCTGACCGAGCAAATCGCTTATTTCACCGGCCCAACAGTTGGGGGGCTTTTGAATGCAACATGTGGGAATGCGACGGAGCTGATAATTGCAATTCTGGCGCTCACACAAGGAAAGATAGACGTGGTGAAGTTCTCTCTTTTGGGCTCTGTTCTTTCCAACCTTCTTCTTGTTCTTGGCACCTCTCTCTTCTGTGGTGGCATCATTAATAAAGAGCAGAGTTATGACAGAAGGCAAGCGGATGTGAACGCACTGATGCTGATGCTGTCATTACTGTGCCACCTATTGCCGTTATTGTTTGGGTACGCCGCCTCCTCCGCCTCCTCCGCCTCCACGGCCGCCGCCTCCACCCTCCACTTGTCCAGAGCCAGCAGCATTGTAATGCTCGCCGCTTACATTGCTTACTTGGTCTTCCAACTCTGGACTCACCGGACATTATTTGAAACTGAGGAAACCGAAGACGACGACGTTTCTGGAGAAGAGGCAGTGATCGGATTCTGGAGTGCATTTGCATGGCTTGCTGGAATGACACTTCTTATTGCTTTGCTGTCCGAATACGTTGTGAACACAATTGAGGAAGCATCTACTACGTGGGGTTTGTCGGTCAGTTTCCTTAGCATAATCTTGCTCCCTATCGTCGGAAATGCAGCCGAACACGCCGGGGCTATCATATTCGCCTTCAAGAATAAGTTGGACATATCTTTGGGAGTTGCATTGGGATCTGCAACACAAATTGCACTTTTTGTGATTCCACTGTGTGTGATAGTTGCTTGGACAATGGGAGTGGACATGGACCTGAACTTTAACCTCATGGAAACGGGGTCGCTTGGGTTGGCCATTATTGCCACGGCCTTCACTTTACAAGATGGAACCTCTCACTATATTAAAGGCCTCATTCTTCTCCTCTGCTACATCATCATTGCTGCTTGCTTCTTTGTTTCCAAGACCCAAGAAGAATTGGCCTGGATCGAACGCTTGCCGCCTGCCTGGTGGAACGCTTAG

Protein sequence

MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIINKEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAACFFVSKTQEELAWIERLPPAWWNA
Homology
BLAST of CmoCh03G003620 vs. ExPASy Swiss-Prot
Match: Q93Z81 (Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.7e-161
Identity = 315/438 (71.92%), Postives = 362/438 (82.65%), Query Frame = 0

Query: 1   MASQSEPW--LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHN+SSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+  L NLQEVILGTKL++LF AIPLAI+A S+ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLF 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+ VVK+SLLGS+LSNLLLVLGTSLF
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 CGGIIN--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSR 240
            GGI N  +EQ +DR+QADVN  +L++ LLCHLLPLL  YAA+   S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLAAYIAYLVFQLWTHRTLFETEETE----DDDVSGEEA-VIGFWSAFAWLAGMT 300
            SSIVML AYIAYL+FQLWTHR LFE ++ +    DD+VS EE  VIGFWS FAWL GMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           ++IALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 420
           ALGSATQI+LFV+PL VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

Query: 421 LILLLCYIIIAACFFVSK 426
           L+LLLCY+IIAACFFV +
Sbjct: 421 LVLLLCYVIIAACFFVDQ 436

BLAST of CmoCh03G003620 vs. ExPASy Swiss-Prot
Match: Q39253 (Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 SV=3)

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-158
Identity = 304/435 (69.89%), Postives = 353/435 (81.15%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGII 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVK+SLLGS+LSNLLLVLGTSLFCGGI 
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 N--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
           N  +EQ +DR+QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 424
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

Query: 425 IIIAACFFVSKTQEE 430
            IIA CFFV K  ++
Sbjct: 426 FIIAICFFVDKLPQK 438

BLAST of CmoCh03G003620 vs. ExPASy Swiss-Prot
Match: Q769E5 (Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1a PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 5.3e-147
Identity = 286/422 (67.77%), Postives = 337/422 (79.86%), Query Frame = 0

Query: 10  MENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQEVILGT 69
           ME G  ++L     HG  RTAHN+SSSSLRKKSD  LV K+    LR  L NLQEV L T
Sbjct: 7   MEAG--RKLAARHPHGRSRTAHNMSSSSLRKKSDAALVRKVPVAPLRPLLANLQEVFLAT 66

Query: 70  KLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPTVGGLLN 129
           KL++LFPA+PLAI AQ F F + W+FALSLLGL PLAERVSFLTEQIA +TGPTVGGLLN
Sbjct: 67  KLAVLFPAVPLAIAAQCFRFDQVWVFALSLLGLIPLAERVSFLTEQIALYTGPTVGGLLN 126

Query: 130 ATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN--KEQSYDR 189
           ATCGNATELIIA+ AL +GKI+VVK SLLGSVLSNLLLVLGTSLFCGG++N    Q YDR
Sbjct: 127 ATCGNATELIIALFALLKGKIEVVKCSLLGSVLSNLLLVLGTSLFCGGVVNLGARQPYDR 186

Query: 190 RQADVNALMLMLSLLCHLLPLLFGY--AASSASSASTAAASTLHLSRASSIVMLAAYIAY 249
            Q+DV+  +L L++LCH  PLL  Y  AA   S ++T+AA++L LSRA S VMLA+Y+AY
Sbjct: 187 NQSDVSTALLFLAVLCHSAPLLLRYAVAAGEHSVSATSAAASLDLSRACSFVMLASYVAY 246

Query: 250 LVFQLWTHRTLFETEETEDDDV--SGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNTIEE 309
           L FQL THR LFE +E +  D     EE  +GF SA  WLA MT +I++LSEYVV TIE 
Sbjct: 247 LFFQLKTHRQLFEPQEVDGGDAGDDDEEPALGFASALFWLALMTAVISVLSEYVVGTIEP 306

Query: 310 ASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVIPLC 369
            S +WGLSVSF+SIILLPIVGNAAEHAGAIIFA KNKLDI+LGVALGSATQI++FV+PL 
Sbjct: 307 TSQSWGLSVSFISIILLPIVGNAAEHAGAIIFALKNKLDITLGVALGSATQISMFVVPLS 366

Query: 370 VIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAACFFV 426
           V+VAW MGV MDL+F L+ETGSL +A++ TAFTLQDGTSHY+KG++LLLCYI+I ACFFV
Sbjct: 367 VLVAWIMGVQMDLDFKLLETGSLFMAVLVTAFTLQDGTSHYLKGILLLLCYIVIGACFFV 426

BLAST of CmoCh03G003620 vs. ExPASy Swiss-Prot
Match: Q5TKG3 (Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=CAX1b PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.9e-137
Identity = 271/428 (63.32%), Postives = 328/428 (76.64%), Query Frame = 0

Query: 9   LMENGTLKRLTKESRH-----GHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINLQ 68
           +ME+ T+ +      H        RTAHNLSSSSLRK SD +L+ K+    LR  L NL 
Sbjct: 7   MMESNTMAKEMAVRHHLLPGSPRRRTAHNLSSSSLRKSSDASLLHKVPCAALRSLLANLN 66

Query: 69  EVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGPT 128
           +V+L T+L +LFPA+ LAI A    FG+ W+F LSL+GL PLAER+SFLTEQIA++TGPT
Sbjct: 67  DVLLTTRLFLLFPAVLLAIAATYLHFGQVWVFVLSLIGLVPLAERLSFLTEQIAFYTGPT 126

Query: 129 VGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN--K 188
           VGGLLNAT GN TE+IIA+LAL +GKI+VVK SLLGS+LSNLLLVLGTSLF  GI N   
Sbjct: 127 VGGLLNATFGNVTEVIIALLALREGKIEVVKCSLLGSILSNLLLVLGTSLFLAGIANLRA 186

Query: 189 EQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLAA 248
            Q YD +QA VN  +LML++LCH LPL+  YA +S   A  +  + LHLSRA SI+ML A
Sbjct: 187 HQPYDTKQAHVNTALLMLAVLCHSLPLMLRYAVTSGDHAIVSGDAALHLSRACSILMLIA 246

Query: 249 YIAYLVFQLWTHRTLFETEETEDDD------VSGEEAVIGFWSAFAWLAGMTLLIALLSE 308
           Y+AYL FQL THR LFE ++ EDDD         +E V+GF SA  WLA MTLL ALLS 
Sbjct: 247 YLAYLFFQLNTHRQLFEPQQVEDDDDDDLVIAQDDEPVLGFSSAMIWLALMTLLTALLSG 306

Query: 309 YVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQI 368
           YVV+TIE AS +W LSVSF+SIILLPIVGNAAEHAGA+IFA KNK+DI+LGV+LGSATQI
Sbjct: 307 YVVSTIEAASESWELSVSFISIILLPIVGNAAEHAGAVIFALKNKMDITLGVSLGSATQI 366

Query: 369 ALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYI 424
           ++FV+P+ VIVAWTMG+ MDL+FNL+ETGSL LAI+ TAFTLQ+G SHY+KGLIL+LCY 
Sbjct: 367 SMFVVPVSVIVAWTMGIPMDLDFNLLETGSLFLAILVTAFTLQEGESHYLKGLILVLCYA 426

BLAST of CmoCh03G003620 vs. ExPASy Swiss-Prot
Match: Q945S5 (Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 SV=2)

HSP 1 Score: 473.0 bits (1216), Expect = 3.7e-132
Identity = 263/439 (59.91%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 3   SQSEPWLMEN---GTLKRLTKESRHGHGRTAHNLSSSSL-RKKSDLTLVSKIQFGCLRKF 62
           S S   L+EN   G+ K   + SR    R    +S+SSL RK+SDL L+S++++  +R+ 
Sbjct: 8   SSSNLSLLENGGGGSDKPTAETSR----RVRRTVSASSLIRKRSDLKLISRVRWEFMRRI 67

Query: 63  LINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAY 122
           L NLQEV+LGTKL ILFPA+PLA+VA  +   R W+FALSLLGLTPLAER+SFLTEQIA+
Sbjct: 68  LTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAF 127

Query: 123 FTGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGI 182
            TGPTVGGL+NATCGNATE+IIAILA+ Q K+ +VK SLLGS+LSNLL VLGTSLF GGI
Sbjct: 128 HTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGI 187

Query: 183 IN--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSI 242
            N  K QS+D RQ D+N+++L L+LLC  LP++  +   +  +     +  + LSRASS 
Sbjct: 188 SNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRF---TMEAEEYDGSDVVVLSRASSF 247

Query: 243 VMLAAYIAYLVFQLWTHR------TLFETEETEDDDVSGEE---AVIGFWSAFAWLAGMT 302
           VML AY+A+L+F L++         L + E+  DDDVS +E   AVIG WSA  WL  MT
Sbjct: 248 VMLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMT 307

Query: 303 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 362
           LL+ALLS+Y+V+TI++A+ +WGLSV F+ IILLPIVGNAAEHAGA+IFAF+NKLDI+LG+
Sbjct: 308 LLVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGI 367

Query: 363 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 422
           ALGSATQIALFV+P+ V+VAWTMG++MDLNFNL+ET    L+I+ T+  LQDGTS+Y+KG
Sbjct: 368 ALGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKG 427

Query: 423 LILLLCYIIIAACFFVSKT 427
           L+LLLCY++IAACFFVS +
Sbjct: 428 LVLLLCYVVIAACFFVSNS 439

BLAST of CmoCh03G003620 vs. ExPASy TrEMBL
Match: A0A6J1GDQ2 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 PE=3 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 1.2e-229
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
           KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

Query: 421 FFVSKTQEE 430
           FFVSKTQE+
Sbjct: 421 FFVSKTQED 429

BLAST of CmoCh03G003620 vs. ExPASy TrEMBL
Match: A0A6J1ITD3 (Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.0e-229
Identity = 427/429 (99.53%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATE+IIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN
Sbjct: 121 GPTVGGLLNATCGNATEMIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
           KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

Query: 421 FFVSKTQEE 430
           FFVSKTQE+
Sbjct: 421 FFVSKTQED 429

BLAST of CmoCh03G003620 vs. ExPASy TrEMBL
Match: J7KJQ3 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.5e-216
Identity = 404/431 (93.74%), Postives = 416/431 (96.52%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYA   A+SASTAAASTLHLSRASSIVM
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYA---AASASTAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

Query: 421 ACFFVSKTQEE 430
           ACFFVSKTQE+
Sbjct: 421 ACFFVSKTQED 428

BLAST of CmoCh03G003620 vs. ExPASy TrEMBL
Match: A0A1S3BNT2 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.6e-215
Identity = 405/431 (93.97%), Postives = 414/431 (96.06%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIP AIVA SFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPFAIVAHSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
             +EQ YDRRQADVN+LMLMLSLLCHLLPLLFGYA   ASSASTAAASTLHLSRASSIVM
Sbjct: 181 LRREQIYDRRQADVNSLMLMLSLLCHLLPLLFGYA---ASSASTAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L+AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTLLIALLSEYVV
Sbjct: 241 LSAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420
           VIPLCVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

Query: 421 ACFFVSKTQEE 430
           ACFFVSKTQE+
Sbjct: 421 ACFFVSKTQED 428

BLAST of CmoCh03G003620 vs. ExPASy TrEMBL
Match: A0A6J1DEY3 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.7e-204
Identity = 388/429 (90.44%), Postives = 409/429 (95.34%), Query Frame = 0

Query: 3   SQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLINL 62
           +Q+EPWL+ENGTLKRLTK+SRHGHGRTAHN+SSSSLRKKSDLTLVSKI+F CLR FLINL
Sbjct: 5   TQTEPWLLENGTLKRLTKDSRHGHGRTAHNMSSSSLRKKSDLTLVSKIRFPCLRNFLINL 64

Query: 63  QEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFTGP 122
           QEVILGTKLSILFPAIPLAI+AQ F FGRPW+FALSLLGLTPLAERVSFLTEQIAYFTGP
Sbjct: 65  QEVILGTKLSILFPAIPLAIIAQIFRFGRPWVFALSLLGLTPLAERVSFLTEQIAYFTGP 124

Query: 123 TVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN-- 182
           TVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N  
Sbjct: 125 TVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIANMR 184

Query: 183 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 242
            EQ+YDRRQADVNALMLMLSLLCHLLPLLFGYA   ASSA+TAAASTLHLSRASSIVML+
Sbjct: 185 TEQNYDRRQADVNALMLMLSLLCHLLPLLFGYA---ASSAATAAASTLHLSRASSIVMLS 244

Query: 243 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 302
           AYIAYLVFQLWTHR LFE E+ +DD VS EEAVIGF SAFAWL GMT+LIALLSEYVVN+
Sbjct: 245 AYIAYLVFQLWTHRQLFE-EDGDDDVVSDEEAVIGFGSAFAWLIGMTILIALLSEYVVNS 304

Query: 303 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 362
           IEEAST+WGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDI+LGVALGSATQIALFVI
Sbjct: 305 IEEASTSWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDITLGVALGSATQIALFVI 364

Query: 363 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 422
           P+CVIVAWTMGVDMDLNFN+METGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 365 PMCVIVAWTMGVDMDLNFNIMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 424

Query: 423 FFVSKTQEE 430
           FFVSKTQ +
Sbjct: 425 FFVSKTQPD 429

BLAST of CmoCh03G003620 vs. NCBI nr
Match: XP_022949765.1 (vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuolar cation/proton exchanger 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805.4 bits (2079), Expect = 2.4e-229
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
           KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

Query: 421 FFVSKTQEE 430
           FFVSKTQE+
Sbjct: 421 FFVSKTQED 429

BLAST of CmoCh03G003620 vs. NCBI nr
Match: XP_022978169.1 (vacuolar cation/proton exchanger 3 [Cucurbita maxima])

HSP 1 Score: 804.7 bits (2077), Expect = 4.1e-229
Identity = 427/429 (99.53%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATE+IIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN
Sbjct: 121 GPTVGGLLNATCGNATEMIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
           KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

Query: 421 FFVSKTQEE 430
           FFVSKTQE+
Sbjct: 421 FFVSKTQED 429

BLAST of CmoCh03G003620 vs. NCBI nr
Match: XP_023545142.1 (vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 804.3 bits (2076), Expect = 5.3e-229
Identity = 427/429 (99.53%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI
Sbjct: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180

Query: 181 KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240
           KEQ+YDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA
Sbjct: 181 KEQTYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVMLA 240

Query: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300
           AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT
Sbjct: 241 AYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVVNT 300

Query: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360
           IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI
Sbjct: 301 IEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALFVI 360

Query: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420
           PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC
Sbjct: 361 PLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIAAC 420

Query: 421 FFVSKTQEE 430
           FFVSKTQE+
Sbjct: 421 FFVSKTQED 429

BLAST of CmoCh03G003620 vs. NCBI nr
Match: NP_001267681.1 (vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuolar cation/proton exchanger 1-like isoform X1 [Cucumis sativus] >AFQ99295.1 cation exchanger CAX3 [Cucumis sativus] >KGN54380.1 hypothetical protein Csa_018138 [Cucumis sativus])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 404/431 (93.74%), Postives = 416/431 (96.52%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENGTLKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQFGCLRKF I
Sbjct: 1   MASQTEPWLMENGTLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQFGCLRKFCI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQ+FGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQNFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYA   A+SASTAAASTLHLSRASSIVM
Sbjct: 181 IRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYA---AASASTAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTL+IALLSEYVV
Sbjct: 241 LTAYIAYLVFQLWTHRQLFEAEEGEDDDVPGEEAVIGFWSAFAWLVGMTLVIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420
           VIP+CVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPMCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

Query: 421 ACFFVSKTQEE 430
           ACFFVSKTQE+
Sbjct: 421 ACFFVSKTQED 428

BLAST of CmoCh03G003620 vs. NCBI nr
Match: XP_038882514.1 (vacuolar cation/proton exchanger 3-like [Benincasa hispida])

HSP 1 Score: 758.4 bits (1957), Expect = 3.3e-215
Identity = 404/431 (93.74%), Postives = 416/431 (96.52%), Query Frame = 0

Query: 1   MASQSEPWLMENGTLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFLI 60
           MASQ+EPWLMENG+LKRLTKE RHGHGRTAHN+SSSSLRKKSDLTLVSKIQ GCLRKFLI
Sbjct: 1   MASQTEPWLMENGSLKRLTKEHRHGHGRTAHNMSSSSLRKKSDLTLVSKIQLGCLRKFLI 60

Query: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120
           NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT
Sbjct: 61  NLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYFT 120

Query: 121 GPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGIIN 180
           GPTVGGLLNATCGNATELIIAILALTQ KIDVVK+SLLGSVLSNLLLVLGTSLFCGGI N
Sbjct: 121 GPTVGGLLNATCGNATELIIAILALTQRKIDVVKYSLLGSVLSNLLLVLGTSLFCGGIAN 180

Query: 181 --KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIVM 240
             +EQ+YDRRQADVN+LMLMLSLLCHLLPLLFGYA   ASS +TAAASTLHLSRASSIVM
Sbjct: 181 LRREQNYDRRQADVNSLMLMLSLLCHLLPLLFGYA---ASSTATAAASTLHLSRASSIVM 240

Query: 241 LAAYIAYLVFQLWTHRTLFETEETEDDDVSGEEAVIGFWSAFAWLAGMTLLIALLSEYVV 300
           L+AYIAYLVFQLWTHR LFE EE EDDDV GEEAVIGFWSAFAWL GMTLLIALLSEYVV
Sbjct: 241 LSAYIAYLVFQLWTHRQLFEAEEVEDDDVPGEEAVIGFWSAFAWLVGMTLLIALLSEYVV 300

Query: 301 NTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360
           NTIEEAS+TWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF
Sbjct: 301 NTIEEASSTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQIALF 360

Query: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCYIIIA 420
           VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGL+LLLCYIIIA
Sbjct: 361 VIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLVLLLCYIIIA 420

Query: 421 ACFFVSKTQEE 430
           ACFFVSKTQE+
Sbjct: 421 ACFFVSKTQED 428

BLAST of CmoCh03G003620 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 570.5 bits (1469), Expect = 1.2e-162
Identity = 315/438 (71.92%), Postives = 362/438 (82.65%), Query Frame = 0

Query: 1   MASQSEPW--LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGC 60
           M S  EPW  + ENG    T K  ++E R  HGRTAHN+SSSSLRKKSDL LV K+    
Sbjct: 1   MGSIVEPWAAIAENGNANVTAKGSSRELR--HGRTAHNMSSSSLRKKSDLRLVQKVPCKT 60

Query: 61  LRKFLINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTE 120
           L+  L NLQEVILGTKL++LF AIPLAI+A S+ +GRP IF LSL+GLTPLAERVSFLTE
Sbjct: 61  LKNILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLAERVSFLTE 120

Query: 121 QIAYFTGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLF 180
           Q+A++TGPTVGGLLNATCGNATELIIAILAL   K+ VVK+SLLGS+LSNLLLVLGTSLF
Sbjct: 121 QLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLLLVLGTSLF 180

Query: 181 CGGIIN--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSR 240
            GGI N  +EQ +DR+QADVN  +L++ LLCHLLPLL  YAA+   S S     +L LSR
Sbjct: 181 FGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMINKMSLTLSR 240

Query: 241 ASSIVMLAAYIAYLVFQLWTHRTLFETEETE----DDDVSGEEA-VIGFWSAFAWLAGMT 300
            SSIVML AYIAYL+FQLWTHR LFE ++ +    DD+VS EE  VIGFWS FAWL GMT
Sbjct: 241 TSSIVMLIAYIAYLIFQLWTHRQLFEAQQDDDDAYDDEVSVEETPVIGFWSGFAWLVGMT 300

Query: 301 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360
           ++IALLSEYVV+TIE+AS +WGLSVSF+SIILLPIVGNAAEHAGAIIFAFKNKLDISLGV
Sbjct: 301 IVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 360

Query: 361 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 420
           ALGSATQI+LFV+PL VIVAW +G+ MDLNFN++ET SL LAII TAFTLQDGTSHY+KG
Sbjct: 361 ALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIITAFTLQDGTSHYMKG 420

Query: 421 LILLLCYIIIAACFFVSK 426
           L+LLLCY+IIAACFFV +
Sbjct: 421 LVLLLCYVIIAACFFVDQ 436

BLAST of CmoCh03G003620 vs. TAIR 10
Match: AT2G38170.1 (cation exchanger 1 )

HSP 1 Score: 561.2 bits (1445), Expect = 7.3e-160
Identity = 304/435 (69.89%), Postives = 353/435 (81.15%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGII 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVK+SLLGS+LSNLLLVLGTSLFCGGI 
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 N--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
           N  +EQ +DR+QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 424
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

Query: 425 IIIAACFFVSKTQEE 430
            IIA CFFV K  ++
Sbjct: 426 FIIAICFFVDKLPQK 438

BLAST of CmoCh03G003620 vs. TAIR 10
Match: AT2G38170.3 (cation exchanger 1 )

HSP 1 Score: 561.2 bits (1445), Expect = 7.3e-160
Identity = 307/439 (69.93%), Postives = 355/439 (80.87%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGII 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVK+SLLGS+LSNLLLVLGTSLFCGGI 
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 N--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
           N  +EQ +DR+QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGVALGSATQ 364
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKLDISLGVALGSATQ
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLDISLGVALGSATQ 365

Query: 365 IALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKGLILLLCY 424
           I LFV+PL +IVAW +G++MDLNF  +ETG L ++II TAFTLQDG+SHY+KGL+LLLCY
Sbjct: 366 IGLFVVPLTIIVAWILGINMDLNFGPLETGCLAVSIIITAFTLQDGSSHYMKGLVLLLCY 425

Query: 425 IIIAACFFVSK-TQEELAW 433
            IIA CFFV K  Q EL +
Sbjct: 426 FIIAICFFVDKLPQSELVF 442

BLAST of CmoCh03G003620 vs. TAIR 10
Match: AT5G01490.1 (cation exchanger 4 )

HSP 1 Score: 473.0 bits (1216), Expect = 2.6e-133
Identity = 263/439 (59.91%), Postives = 338/439 (76.99%), Query Frame = 0

Query: 3   SQSEPWLMEN---GTLKRLTKESRHGHGRTAHNLSSSSL-RKKSDLTLVSKIQFGCLRKF 62
           S S   L+EN   G+ K   + SR    R    +S+SSL RK+SDL L+S++++  +R+ 
Sbjct: 8   SSSNLSLLENGGGGSDKPTAETSR----RVRRTVSASSLIRKRSDLKLISRVRWEFMRRI 67

Query: 63  LINLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAY 122
           L NLQEV+LGTKL ILFPA+PLA+VA  +   R W+FALSLLGLTPLAER+SFLTEQIA+
Sbjct: 68  LTNLQEVLLGTKLFILFPAVPLAVVAHRYDCPRAWVFALSLLGLTPLAERISFLTEQIAF 127

Query: 123 FTGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGI 182
            TGPTVGGL+NATCGNATE+IIAILA+ Q K+ +VK SLLGS+LSNLL VLGTSLF GGI
Sbjct: 128 HTGPTVGGLMNATCGNATEMIIAILAVGQRKMRIVKLSLLGSILSNLLFVLGTSLFLGGI 187

Query: 183 IN--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSI 242
            N  K QS+D RQ D+N+++L L+LLC  LP++  +   +  +     +  + LSRASS 
Sbjct: 188 SNLRKHQSFDPRQGDMNSMLLYLALLCQTLPMIMRF---TMEAEEYDGSDVVVLSRASSF 247

Query: 243 VMLAAYIAYLVFQLWTHR------TLFETEETEDDDVSGEE---AVIGFWSAFAWLAGMT 302
           VML AY+A+L+F L++         L + E+  DDDVS +E   AVIG WSA  WL  MT
Sbjct: 248 VMLIAYLAFLIFHLFSSHLSPPPPPLPQREDVHDDDVSDKEEEGAVIGMWSAIFWLIIMT 307

Query: 303 LLIALLSEYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISLGV 362
           LL+ALLS+Y+V+TI++A+ +WGLSV F+ IILLPIVGNAAEHAGA+IFAF+NKLDI+LG+
Sbjct: 308 LLVALLSDYLVSTIQDAADSWGLSVGFIGIILLPIVGNAAEHAGAVIFAFRNKLDITLGI 367

Query: 363 ALGSATQIALFVIPLCVIVAWTMGVDMDLNFNLMETGSLGLAIIATAFTLQDGTSHYIKG 422
           ALGSATQIALFV+P+ V+VAWTMG++MDLNFNL+ET    L+I+ T+  LQDGTS+Y+KG
Sbjct: 368 ALGSATQIALFVVPVTVLVAWTMGIEMDLNFNLLETACFALSILVTSLVLQDGTSNYMKG 427

Query: 423 LILLLCYIIIAACFFVSKT 427
           L+LLLCY++IAACFFVS +
Sbjct: 428 LVLLLCYVVIAACFFVSNS 439

BLAST of CmoCh03G003620 vs. TAIR 10
Match: AT2G38170.2 (cation exchanger 1 )

HSP 1 Score: 437.2 bits (1123), Expect = 1.6e-122
Identity = 243/351 (69.23%), Postives = 280/351 (79.77%), Query Frame = 0

Query: 5   SEPW-LMENG----TLKRLTKESRHGHGRTAHNLSSSSLRKKSDLTLVSKIQFGCLRKFL 64
           +EPW + ENG    T K  ++E R   GRTAHN+SSSSLRKKSDL ++ K+ +  L+ FL
Sbjct: 6   TEPWSVAENGNPSITAKGSSRELR--LGRTAHNMSSSSLRKKSDLRVIQKVPYKGLKDFL 65

Query: 65  INLQEVILGTKLSILFPAIPLAIVAQSFGFGRPWIFALSLLGLTPLAERVSFLTEQIAYF 124
            NLQEVILGTKL+ILFPAIP AI+    G  +PWIF LSLLGLTPLAERVSFLTEQ+A++
Sbjct: 66  SNLQEVILGTKLAILFPAIPAAIICTYCGVSQPWIFGLSLLGLTPLAERVSFLTEQLAFY 125

Query: 125 TGPTVGGLLNATCGNATELIIAILALTQGKIDVVKFSLLGSVLSNLLLVLGTSLFCGGII 184
           TGPT+GGLLNATCGNATELIIAILALT  K+ VVK+SLLGS+LSNLLLVLGTSLFCGGI 
Sbjct: 126 TGPTLGGLLNATCGNATELIIAILALTNNKVAVVKYSLLGSILSNLLLVLGTSLFCGGIA 185

Query: 185 N--KEQSYDRRQADVNALMLMLSLLCHLLPLLFGYAASSASSASTAAASTLHLSRASSIV 244
           N  +EQ +DR+QADVN  +L+L  LCHLLPLL GY  +  +SA+  +   L +SR  SIV
Sbjct: 186 NIRREQRFDRKQADVNFFLLLLGFLCHLLPLLVGYLKNGEASAAVLSDMQLSISRGFSIV 245

Query: 245 MLAAYIAYLVFQLWTHRTLFETEETE---DDDVSGEEAVIGFWSAFAWLAGMTLLIALLS 304
           ML +YIAYLVFQLWTHR LF+ +E E   DDDV  E AVI FWS FAWL GMTL+IALLS
Sbjct: 246 MLISYIAYLVFQLWTHRQLFDAQEQEDEYDDDVEQETAVISFWSGFAWLVGMTLVIALLS 305

Query: 305 EYVVNTIEEASTTWGLSVSFLSIILLPIVGNAAEHAGAIIFAFKNKLDISL 346
           EYVV TIEEAS  W LSVSF+SIILLPIVGNAAEHAGA+IFAFKNKL  S+
Sbjct: 306 EYVVATIEEASDKWNLSVSFISIILLPIVGNAAEHAGAVIFAFKNKLVSSI 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Z811.7e-16171.92Vacuolar cation/proton exchanger 3 OS=Arabidopsis thaliana OX=3702 GN=CAX3 PE=1 ... [more]
Q392531.0e-15869.89Vacuolar cation/proton exchanger 1 OS=Arabidopsis thaliana OX=3702 GN=CAX1 PE=1 ... [more]
Q769E55.3e-14767.77Vacuolar cation/proton exchanger 1a OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q5TKG32.9e-13763.32Vacuolar cation/proton exchanger 1b OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q945S53.7e-13259.91Vacuolar cation/proton exchanger 4 OS=Arabidopsis thaliana OX=3702 GN=CAX4 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GDQ21.2e-22999.77Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111453055 P... [more]
A0A6J1ITD32.0e-22999.53Vacuolar cation/proton exchanger OS=Cucurbita maxima OX=3661 GN=LOC111478234 PE=... [more]
J7KJQ39.5e-21693.74Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX3 PE=2 SV=1[more]
A0A1S3BNT21.6e-21593.97Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103491652 PE=3 SV... [more]
A0A6J1DEY31.7e-20490.44Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111019448 ... [more]
Match NameE-valueIdentityDescription
XP_022949765.12.4e-22999.77vacuolar cation/proton exchanger 3-like [Cucurbita moschata] >KAG6603584.1 Vacuo... [more]
XP_022978169.14.1e-22999.53vacuolar cation/proton exchanger 3 [Cucurbita maxima][more]
XP_023545142.15.3e-22999.53vacuolar cation/proton exchanger 3-like [Cucurbita pepo subsp. pepo][more]
NP_001267681.12.0e-21593.74vacuolar cation/proton exchanger 1-like [Cucumis sativus] >XP_011652900.1 vacuol... [more]
XP_038882514.13.3e-21593.74vacuolar cation/proton exchanger 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G51860.11.2e-16271.92cation exchanger 3 [more]
AT2G38170.17.3e-16069.89cation exchanger 1 [more]
AT2G38170.37.3e-16069.93cation exchanger 1 [more]
AT5G01490.12.6e-13359.91cation exchanger 4 [more]
AT2G38170.21.6e-12269.23cation exchanger 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 272..423
e-value: 2.5E-13
score: 51.9
coord: 122..271
e-value: 1.6E-14
score: 55.9
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 93..251
e-value: 1.8E-22
score: 79.9
coord: 279..420
e-value: 1.1E-16
score: 61.2
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 76..422
e-value: 5.2E-96
score: 319.8
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 58..422
e-value: 3.4E-142
score: 471.6
NoneNo IPR availablePANTHERPTHR31503:SF1VACUOLAR CATION/PROTON EXCHANGER 3coord: 4..430
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 4..430

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G003620.1CmoCh03G003620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070588 calcium ion transmembrane transport
biological_process GO:0006874 cellular calcium ion homeostasis
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity