CmoCh03G003270 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G003270
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionSolute carrier family 40 protein
LocationCmo_Chr03: 4429627 .. 4433706 (+)
RNA-Seq ExpressionCmoCh03G003270
SyntenyCmoCh03G003270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCCAGCTCATATAAGAACCTCCATATTTACAAAGACCCATAAGAAAAAATTGTCGCTTCCCTGAATATCCATTCCCTCAGAGACGCATAAAAGAGACCCACCTTTTTCTAATCATCGATCGAAGAGCAGCAAGCAATTTCCCAAGTTAAAAAAGGAAGAATTCCCCGTCTGGGAATTCGGAAGTCAAACTCCCACTGATTTCGTTCTGTTTACCCTTTTCTTTGATCGATTCAGTTCATATTTCGTTAATCTCAGATTGATTTTGATCCAGAGGAGCTGTGATTGAAGAAAACAGTAATGGAGAATGAGCCGCTTCTGTCCCCTCTGCAATCCCCTGCTTCTTCTTCTCTTCTTCGGAGTCTGTACGCCGGACACTTCTTGGCCAGATGGGGCGCTAGGTTTTCCCCCTTTCTTCTCGCTATTTTGGTTTCCATTCCGATTATTCCCGTCATTTCATAGATTTTAGATGAACCTAGTTGTTTGTTTGATAGAATGATTTGAATCCCTGTTTTTGGTTTGATCTTATCATGTTTTAGGATTTGATTTCATTATATCAGGATTGAAAACAGTGTAAAAGAAACTTGGGATTTGTTTTGTTTTGTTTCGAACGTTTTCTTCCATTGTTGCACAGATTGCACACTAAGTGTTTGTATTTATGTCTCTTAGAGCAGATTTTGGTTTTGTTTTTTAAAACTCGAGTTCTGTTCCAACCCTTTTGATTGGATTTGGATTTGTTCTTACTGCCTTTGATTTTGTGACAGAATGTGGGAATTCTCCGTTGGTTTATATATGATCAGTGTGTGGCCCAATTCTCTACTCTTTGCAGCGATTTATGGTGTTGTGGAATCTGCTTCCACTGCTTTCTTTGGTCCAATTGTTGGTGATTGGGTTGATAAGTTAGCATACGTCAAGGTTTTGCTCTCTTTTTGCTTACATTTTCAAAAGAAGAAATGATAACTTATGATCTCCACAATGGTATGGTATTGTCAACTTTAGTTTTACTTTTAGTTTCCCTAAAAGGCCTCGTATGAATGGAGATGTATTTCTTACTTATAAACTCATGATCATTCCTTACCCCCTCCCAACAATCCTCGCCTCGAACAAAGTACACCATAAAGCCTCCCTTAAGGCTTATGGAGCCCTCGAACAACCTCCCCTTAATCGAGGCTCGACTCTTCTAGAGTCGTCGAACAAAGTACACCCTTTGTTCGACACTTGTGTCACTTTTGACCACACCTTCGAGGCTCACAACTTTTTTAATTTACATTTGAAGATTTTATTGACATAACTAAGTTAAGGACATGGCTCTGGTACCATGTTAGGAATAACGACTCTCCACAATGGTATGATATTGTCTACTATGAGCATAAGATCTCATGGTTTTGCTTTGGGCTTCTCCAAAGGGCCTCATACGAATAGAGATGTATTCCTTACTTATAAACCCATGATCATTCCCTAAATTAGCCAACGTGGGACTCCCTCCCAACAATCCTCAACACAATATGCTCATCCATCCTAGATATAAGCACAAGCCAGGTTATATGGGTTTATCATGATCATCTACTTGAATGAAACTAAACAACCATGAATCAATGAAATGATCAAGGTAGTCTTCAAATATCCTCTGGATGAAACAGTTGCTGTTTGCCTGATTGAAGTAGTCGTATATTTTTGGTTCTATGTTATCAGGTTCTCAAAATTTGGCTGGTGACACAAAATCTTTCGTATATTATTGCTGGAGCGACGGTGGTCGCGTTACTGTTCTACTCAGATTTGATATCCACCTATTTCACAGGGTTCATTTTGCTTGTGATATTGACAAATATTGCTGGAGCTGTTGGCGCGCTTTCGTCTCTTGCGGGTACTATCTTAATTGAAAGAGAATGGTAATTGATTGCTGTTTAGTTTTGAATAGCATTTGAGAAGAAAGTTCTATCCAAGTGATGTTTAAGTTGCCAGTTTGGTTTTTGCACAGGGTGGTAGTGATATCAGAACGCCATCCTCCTGAAATGCTGACAAATATAAACTCTACGATGAGGCGGATCGATCTGGTCTGCAAGCTGTTTAGTCCTGTAATATCTGGTTTCATTATTAGTTTCGTTTCTTTGAAGGCATCTGCTGTGACTTTGGCAGTTTGGAATATCGTATCGGTTTGGTTCGAGTATTGGTTGTTCACTTCTGTGTATGATGGGATTCCGGCTTTGGAAGAAAGCAGCCATAGAAGGGTCTCAAGACTTGTTCTAAGAGATGTGGAAGGGAGTAGTTCTGTCTCTCGATTTCCGGATTACGAAGATGGACATTCGGCTGAAACGAGCTGGAAAGTGAAAATGTTCAACTGGTTCTCAAAGTTCCCATTTGTTAGTGCATGGAAGATGTATCTAGAGCAAGACACCGTACTCCCGGGGATCGCTCTCGCTGTGTTATTCTTCACAGTCCTTAGGTAATGTCTGAATCCACTTGCTTTACCAATGCACTCATTAGAACGTGACTGCAAGGGAGAAGTTTCCACACCCTTGTAAGATATATTTCGTTCTCCTCTCTTATCGATGTGGGATCTTATAATCCACTCCGTTGGGGCACAACGTCCTCACTGGCACACCGCCTGATGACCGACTCTGATACTACTGTAACAGTCCAAGCCCTCTGCTAGGAGATATTGTTCGCTTTAGCTTGTTACGTATTATCGTTAGCCTCACGGTTAGGGAGAAGTTTCCACACCCTTATAAGATATGTTTTGTTCTCTTCTCCAATCGAGGTAGGATATCAAGGTAAAGGTTACTCGATGTTTTCACTGATAAAACTATGAGGTTGACGGCGATACGTAACAGACCAAAGAGGATAATATCTGCTAGCGGTGGTCTCAGACTGTCACAAATGGTATCAGAGTCAAGTCACCCGACAATGCGAGATACTGGTTGAAATAGGGCTAAACCCTTTTCATGGTAGATGCGTTTTAAAACGGTGAGGCTGATGGTGATACGTAACGGGCCAAAGCGGACAATATCTACTAGCGGTGGACTTAGGTGTTACATTGATACTCAAGTCTCCCATTCTCAAACTTTTTTCTCTTCCAGCTTTGGAACTTTAATGACTGCAACCTTAGAATGGGAAGGGATACCCGCCTATATCATTGGAATTGCACGTGGAATAAGCGCTACCATTGGCATTGCAGCAACACTTGTTTACCCTATTATACAGTCTCGAATTTCGACGCTTCGAACAGGCCTCTGGTCTATTTGGTCTCAGGTACGTTTAAGCGTCGTTGTAGCCATTGGCTAACAAGAGACCGTTGACATCGAAATGAAAATCTCTCTTTATCAACCGACCAAAACGGTCATTAATATTGATTGTCAATGACATTGCAGTGGTCCTGCTTACTAGTGTGCGTTGCTTCAATATGGATGCAAAACAGCCTCTTGTCAGCATACATGCTAATGATCGGAGTGGCAGTGTCTCGGCTCGGACTCTGGATGTTCGATTTGGCAGTCATCCAACAAATGCAGGTACGAAACACAGCATTCAAATCACAATAGAATTTGCTTCATATTATCTATAATGTAGTTTGATGTAATGAATGGGGAGTTCTTTTGTTTGCTTGCGTTTCATATCATTAGGATCAAGTCCCTGAATCCGATCGTTGCGTCGTCGGGGGTGCACAAAACGCTCTCCAATCGACCATGGACCTGATGGGGTACGTTATGGGAATTGTCGTCTCAAATCCTCAGGTGATACCATCCTCATCATCATTGAAGAACAGTCTATGATGAAGTAGTTATTTTGTATTTGTGTATGACTGTTGGTTATGGTGGCCATTTTTTTTGCAGGATTTCTGGAAGCTGATTCTGATATCGTTCACGGTGGTGACTTTGGCGGCGCTGCTCTATACCGTCCACCTCTACCGTGTCCGAAAGCATCTGTTTCACCTGGAGAAGTTGGCTTCCTGCTGCTCGAGATGGTCACCAAGCTTCAATTTTAATCACTCTTTCTAATGAAAGTGAACAAGAATATAGTTTCTTTCTGCTGTGTAAATTGTTTGTGTAACATTCTATGAGAAGAAACGGATCATGTTCGGTTCAAA

mRNA sequence

TCCCCAGCTCATATAAGAACCTCCATATTTACAAAGACCCATAAGAAAAAATTGTCGCTTCCCTGAATATCCATTCCCTCAGAGACGCATAAAAGAGACCCACCTTTTTCTAATCATCGATCGAAGAGCAGCAAGCAATTTCCCAAGTTAAAAAAGGAAGAATTCCCCGTCTGGGAATTCGGAAGTCAAACTCCCACTGATTTCGTTCTGTTTACCCTTTTCTTTGATCGATTCAGTTCATATTTCGTTAATCTCAGATTGATTTTGATCCAGAGGAGCTGTGATTGAAGAAAACAGTAATGGAGAATGAGCCGCTTCTGTCCCCTCTGCAATCCCCTGCTTCTTCTTCTCTTCTTCGGAGTCTGTACGCCGGACACTTCTTGGCCAGATGGGGCGCTAGAATGTGGGAATTCTCCGTTGGTTTATATATGATCAGTGTGTGGCCCAATTCTCTACTCTTTGCAGCGATTTATGGTGTTGTGGAATCTGCTTCCACTGCTTTCTTTGGTCCAATTGTTGGTGATTGGGTTGATAAGTTAGCATACGTCAAGGTTCTCAAAATTTGGCTGGTGACACAAAATCTTTCGTATATTATTGCTGGAGCGACGGTGGTCGCGTTACTGTTCTACTCAGATTTGATATCCACCTATTTCACAGGGTTCATTTTGCTTGTGATATTGACAAATATTGCTGGAGCTGTTGGCGCGCTTTCGTCTCTTGCGGGTACTATCTTAATTGAAAGAGAATGGGTGGTAGTGATATCAGAACGCCATCCTCCTGAAATGCTGACAAATATAAACTCTACGATGAGGCGGATCGATCTGGTCTGCAAGCTGTTTAGTCCTGTAATATCTGGTTTCATTATTAGTTTCGTTTCTTTGAAGGCATCTGCTGTGACTTTGGCAGTTTGGAATATCGTATCGGTTTGGTTCGAGTATTGGTTGTTCACTTCTGTGTATGATGGGATTCCGGCTTTGGAAGAAAGCAGCCATAGAAGGGTCTCAAGACTTGTTCTAAGAGATGTGGAAGGGAGTAGTTCTGTCTCTCGATTTCCGGATTACGAAGATGGACATTCGGCTGAAACGAGCTGGAAAGTGAAAATGTTCAACTGGTTCTCAAAGTTCCCATTTGTTAGTGCATGGAAGATGTATCTAGAGCAAGACACCGTACTCCCGGGGATCGCTCTCGCTGTGTTATTCTTCACAGTCCTTAGCTTTGGAACTTTAATGACTGCAACCTTAGAATGGGAAGGGATACCCGCCTATATCATTGGAATTGCACGTGGAATAAGCGCTACCATTGGCATTGCAGCAACACTTGTTTACCCTATTATACAGTCTCGAATTTCGACGCTTCGAACAGGCCTCTGGTCTATTTGGTCTCAGTGGTCCTGCTTACTAGTGTGCGTTGCTTCAATATGGATGCAAAACAGCCTCTTGTCAGCATACATGCTAATGATCGGAGTGGCAGTGTCTCGGCTCGGACTCTGGATGTTCGATTTGGCAGTCATCCAACAAATGCAGGATCAAGTCCCTGAATCCGATCGTTGCGTCGTCGGGGGTGCACAAAACGCTCTCCAATCGACCATGGACCTGATGGGGTACGTTATGGGAATTGTCGTCTCAAATCCTCAGGATTTCTGGAAGCTGATTCTGATATCGTTCACGGTGGTGACTTTGGCGGCGCTGCTCTATACCGTCCACCTCTACCGTGTCCGAAAGCATCTGTTTCACCTGGAGAAGTTGGCTTCCTGCTGCTCGAGATGGTCACCAAGCTTCAATTTTAATCACTCTTTCTAATGAAAGTGAACAAGAATATAGTTTCTTTCTGCTGTGTAAATTGTTTGTGTAACATTCTATGAGAAGAAACGGATCATGTTCGGTTCAAA

Coding sequence (CDS)

ATGGAGAATGAGCCGCTTCTGTCCCCTCTGCAATCCCCTGCTTCTTCTTCTCTTCTTCGGAGTCTGTACGCCGGACACTTCTTGGCCAGATGGGGCGCTAGAATGTGGGAATTCTCCGTTGGTTTATATATGATCAGTGTGTGGCCCAATTCTCTACTCTTTGCAGCGATTTATGGTGTTGTGGAATCTGCTTCCACTGCTTTCTTTGGTCCAATTGTTGGTGATTGGGTTGATAAGTTAGCATACGTCAAGGTTCTCAAAATTTGGCTGGTGACACAAAATCTTTCGTATATTATTGCTGGAGCGACGGTGGTCGCGTTACTGTTCTACTCAGATTTGATATCCACCTATTTCACAGGGTTCATTTTGCTTGTGATATTGACAAATATTGCTGGAGCTGTTGGCGCGCTTTCGTCTCTTGCGGGTACTATCTTAATTGAAAGAGAATGGGTGGTAGTGATATCAGAACGCCATCCTCCTGAAATGCTGACAAATATAAACTCTACGATGAGGCGGATCGATCTGGTCTGCAAGCTGTTTAGTCCTGTAATATCTGGTTTCATTATTAGTTTCGTTTCTTTGAAGGCATCTGCTGTGACTTTGGCAGTTTGGAATATCGTATCGGTTTGGTTCGAGTATTGGTTGTTCACTTCTGTGTATGATGGGATTCCGGCTTTGGAAGAAAGCAGCCATAGAAGGGTCTCAAGACTTGTTCTAAGAGATGTGGAAGGGAGTAGTTCTGTCTCTCGATTTCCGGATTACGAAGATGGACATTCGGCTGAAACGAGCTGGAAAGTGAAAATGTTCAACTGGTTCTCAAAGTTCCCATTTGTTAGTGCATGGAAGATGTATCTAGAGCAAGACACCGTACTCCCGGGGATCGCTCTCGCTGTGTTATTCTTCACAGTCCTTAGCTTTGGAACTTTAATGACTGCAACCTTAGAATGGGAAGGGATACCCGCCTATATCATTGGAATTGCACGTGGAATAAGCGCTACCATTGGCATTGCAGCAACACTTGTTTACCCTATTATACAGTCTCGAATTTCGACGCTTCGAACAGGCCTCTGGTCTATTTGGTCTCAGTGGTCCTGCTTACTAGTGTGCGTTGCTTCAATATGGATGCAAAACAGCCTCTTGTCAGCATACATGCTAATGATCGGAGTGGCAGTGTCTCGGCTCGGACTCTGGATGTTCGATTTGGCAGTCATCCAACAAATGCAGGATCAAGTCCCTGAATCCGATCGTTGCGTCGTCGGGGGTGCACAAAACGCTCTCCAATCGACCATGGACCTGATGGGGTACGTTATGGGAATTGTCGTCTCAAATCCTCAGGATTTCTGGAAGCTGATTCTGATATCGTTCACGGTGGTGACTTTGGCGGCGCTGCTCTATACCGTCCACCTCTACCGTGTCCGAAAGCATCTGTTTCACCTGGAGAAGTTGGCTTCCTGCTGCTCGAGATGGTCACCAAGCTTCAATTTTAATCACTCTTTCTAA

Protein sequence

MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEKLASCCSRWSPSFNFNHSF
Homology
BLAST of CmoCh03G003270 vs. ExPASy Swiss-Prot
Match: O80905 (Solute carrier family 40 member 1 OS=Arabidopsis thaliana OX=3702 GN=IREG1 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 2.9e-170
Identity = 306/480 (63.75%), Postives = 372/480 (77.50%), Query Frame = 0

Query: 22  LYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDWVDKLA 81
           LY G+FLARW AR WEFSV LYMI +WPNSLL AAIYG +ES STA FGPIVG WV+ + 
Sbjct: 41  LYVGYFLARWSARTWEFSVALYMIHLWPNSLLLAAIYGAIESGSTAIFGPIVGQWVEGMD 100

Query: 82  YVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGALSSLA 141
           YVKVL++WL+ QNLSY IAG  V+ LL  SDL S     F +L++LTN+AGA+G LS+LA
Sbjct: 101 YVKVLRLWLLFQNLSYTIAGGAVIKLLLVSDLKSRNLPVFAILIVLTNLAGAIGVLSTLA 160

Query: 142 GTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKASAVTL 201
           GTILIER+W VV+SE HPP +LT +NS +R IDL  KL SPVI+G IISFVSLKASA+T 
Sbjct: 161 GTILIERDWAVVMSEGHPPAVLTKMNSVIRGIDLSSKLLSPVITGLIISFVSLKASAITF 220

Query: 202 AVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSS---SVSRFPDYEDGH 261
           A W  ++ W EYWLF SVY G+PA+  S+ RR+ R   + VEG     SVS  P  E+G+
Sbjct: 221 AAWATITAWVEYWLFISVYSGVPAITRSNERRILRSRTKQVEGRDAPVSVSIVPGTEEGY 280

Query: 262 SAETSWK---VKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLMTATLE 321
           +     +   + + +  SK  FV AW++Y  Q+ VLPG++LA+LFFTVLSFGTLMTATL+
Sbjct: 281 TGNPPSRTGILVILDRMSKSSFVGAWRIYFNQEVVLPGVSLALLFFTVLSFGTLMTATLQ 340

Query: 322 WEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCVASIWM 381
           WEGIP YIIGI RGISAT+G+AATLVYP++QSR+STLRTGLWS WSQWSCLLVCV SIW+
Sbjct: 341 WEGIPTYIIGIGRGISATVGLAATLVYPLMQSRLSTLRTGLWSFWSQWSCLLVCVGSIWV 400

Query: 382 QNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTMDLMGY 441
           +   +++YMLM GVA SRLGLWMFDLAVIQQMQD V ESDRCVVGG QN+LQS +DLM Y
Sbjct: 401 KKDKIASYMLMAGVAASRLGLWMFDLAVIQQMQDLVSESDRCVVGGVQNSLQSALDLMAY 460

Query: 442 VMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEK---LASCCSRWSPS 493
           ++GI+VSNP+DFW L LISF+ V+LA +LYT+HLYR+R H+FHLEK   L  C  +  PS
Sbjct: 461 LLGIIVSNPKDFWILTLISFSTVSLAGMLYTIHLYRIRNHIFHLEKIPLLNKCIFKLLPS 520

BLAST of CmoCh03G003270 vs. ExPASy Swiss-Prot
Match: F4KGN5 (Solute carrier family 40 member 2 OS=Arabidopsis thaliana OX=3702 GN=IREG2 PE=2 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.7e-165
Identity = 297/472 (62.92%), Postives = 369/472 (78.18%), Query Frame = 0

Query: 17  SLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDW 76
           S++ SLY G+FLARWGAR WEFSV LYMI +WPNSL   A+YGVVES S   FGPIVG  
Sbjct: 32  SMVISLYLGYFLARWGARTWEFSVALYMIYLWPNSLFLTAMYGVVESGSATLFGPIVGQM 91

Query: 77  VDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGA 136
           +D ++YVKVL++WLVTQNLS+I+AG  VVALL   DL S  F  F  LV+LTN++GA+G 
Sbjct: 92  IDGMSYVKVLRLWLVTQNLSFIVAGGAVVALLVVPDLKSQNFPVFATLVVLTNLSGAIGV 151

Query: 137 LSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKA 196
           LS+LAGT+LIER+WVVV+SE H P +LT +NS +R IDL  KL SPVI+G IISFVSL+A
Sbjct: 152 LSTLAGTVLIERDWVVVMSEGHSPAVLTRMNSVIRGIDLSSKLLSPVITGLIISFVSLRA 211

Query: 197 SAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSSSVSRF----- 256
           SA+T A W  ++VW EYWLF SVY+G+PA+ +S  RR  R      E + S S F     
Sbjct: 212 SAITFAAWATITVWIEYWLFISVYNGVPAIVQSDERRSLRSSQSQAEETDSASSFYVPLL 271

Query: 257 PDYEDGHSAETSWKV-KMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLM 316
            + E   + ++  ++ ++    S+  FVSAW+ YL Q+ VLPG++LA+LFFTVLSFGTLM
Sbjct: 272 HEEESYRNTQSRSRILRILERISESSFVSAWRNYLNQEIVLPGVSLALLFFTVLSFGTLM 331

Query: 317 TATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCV 376
           TATLEW+GIP YIIGI RGISA +G+AAT++YP++QSRIS LRTG+WS WSQW+CLLVCV
Sbjct: 332 TATLEWKGIPTYIIGIGRGISAGVGLAATVLYPLMQSRISPLRTGVWSFWSQWTCLLVCV 391

Query: 377 ASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTM 436
            SIW++   +++YMLM GVA SRLGLWMFDLAVIQQMQD VPESDRCVVGG QN+LQS +
Sbjct: 392 GSIWVEKEKIASYMLMAGVAASRLGLWMFDLAVIQQMQDLVPESDRCVVGGVQNSLQSAL 451

Query: 437 DLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEKL 483
           DLM  ++GI+VSNP+DFW L LISF  V+LA +LYT+HLYR+RKHLFHLEK+
Sbjct: 452 DLMANLLGIIVSNPKDFWMLTLISFATVSLAGILYTIHLYRIRKHLFHLEKI 503

BLAST of CmoCh03G003270 vs. ExPASy Swiss-Prot
Match: Q5Z922 (Solute carrier family 40 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0560000 PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.7e-154
Identity = 288/481 (59.88%), Postives = 363/481 (75.47%), Query Frame = 0

Query: 5   PLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESA 64
           PLL        ++LLR LY GHFLARWGARMWEFSVGLYMI +WP SLL  A+YGVVE++
Sbjct: 10  PLLDGGGGVDDAALLRRLYVGHFLARWGARMWEFSVGLYMIRIWPGSLLLTAVYGVVEAS 69

Query: 65  STAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFY-SDLISTYFTGFIL 124
           + A  GPIVG  VD+LAY++VL++WL+ Q  S++ AG +V ALL Y + L +  F  F+ 
Sbjct: 70  AVAALGPIVGAVVDRLAYLQVLRLWLLLQGASFVAAGVSVTALLVYGARLAAAGFPAFVA 129

Query: 125 LVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPV 184
           LV++TN++GA+ ALS+LAGTILIEREWVVVI+   P  +LT INS +RRIDL CKL +PV
Sbjct: 130 LVVVTNVSGALAALSTLAGTILIEREWVVVIAGGQPAAVLTGINSVIRRIDLSCKLLAPV 189

Query: 185 ISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVE 244
           +SGF ISFVS++ASA  LA WN+ +VW +YWLF SVY G PAL E+S  ++SR       
Sbjct: 190 LSGFFISFVSMEASAAALAAWNLAAVWVQYWLFVSVYAGFPALSETS--QISR------- 249

Query: 245 GSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTV 304
                 R  D ++  +A    KV+   W +  P   +W +Y  Q+ VLPG+ALA L+FTV
Sbjct: 250 ------RRADDDEAAAAAQPQKVERL-WMTMLPCWESWAVYARQEVVLPGVALAFLYFTV 309

Query: 305 LSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQW 364
           LSFGTLMTATL+WEGIPAY+I +ARG+SA +GIAAT VYP   +R+STLR GLWSIW+QW
Sbjct: 310 LSFGTLMTATLDWEGIPAYVISLARGVSAAVGIAATWVYPAAHARVSTLRAGLWSIWAQW 369

Query: 365 SCLLVCVASIWMQNS--LLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGG 424
            CLLVCVAS+W   +  L SA+MLM GVA SRLGLWMFDLAV+Q MQD VPESDRCVVGG
Sbjct: 370 CCLLVCVASVWAGGAAPLASAWMLMGGVAASRLGLWMFDLAVMQLMQDGVPESDRCVVGG 429

Query: 425 AQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEK 483
            QN+LQS  DL+ YVMGI+VS+P+DF +LI++SF +VT AA +YT+H+YRVRKHLFHL++
Sbjct: 430 VQNSLQSMFDLLTYVMGIIVSDPRDFGELIVLSFFLVTCAAAMYTMHVYRVRKHLFHLDR 474

BLAST of CmoCh03G003270 vs. ExPASy Swiss-Prot
Match: Q9JHI9 (Solute carrier family 40 member 1 OS=Mus musculus OX=10090 GN=Slc40a1 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.7e-58
Identity = 158/514 (30.74%), Postives = 252/514 (49.03%), Query Frame = 0

Query: 22  LYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDWVDKLA 81
           LY GH L+ WG RMW F+V ++++ ++ NSLL  A+YG+V + S    G I+GDWVDK A
Sbjct: 28  LYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNA 87

Query: 82  YVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLV--ILTNIAGAVGALSS 141
            +KV +  LV QN+S I+ G  ++ +  + + + T + G++L V  IL      +  L+S
Sbjct: 88  RLKVAQTSLVVQNVSVILCGIILMMVFLHKNELLTMYHGWVLTVCYILIITIANIANLAS 147

Query: 142 LAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKASAV 201
            A  I I+R+W+VV++  +    L ++N+T+RRID +  + +P+  G I++F S      
Sbjct: 148 TATAITIQRDWIVVVAGEN-RSRLADMNATIRRIDQLTNILAPMAVGQIMTFGSPVIGCG 207

Query: 202 TLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSSSVSRFPDYEDGH- 261
            ++ WN+VS+  EY+L   VY   PAL   +  +V    L+ +         P  E  H 
Sbjct: 208 FISGWNLVSMCVEYFLLWKVYQKTPALAVKAALKVEESELKQLTSPKDTEPKP-LEGTHL 267

Query: 262 -------------SAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLS 321
                          E +   +M   F  F     W  Y  Q   L G+ LA L+ TVL 
Sbjct: 268 MGEKDSNIRELECEQEPTCASQMAEPFRTFR--DGWVSYYNQPVFLAGMGLAFLYMTVLG 327

Query: 322 FGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSC 381
           F  + T     +G+   I+ I  G SA  GI  T+ +  ++ +   +RTGL+S  +Q SC
Sbjct: 328 FDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLFSGLAQLSC 387

Query: 382 LLVCVASIWMQNS----------------------------------------------- 441
           L++CV S++M  S                                               
Sbjct: 388 LILCVISVFMPGSPLDLSVSPFEDIRSRFVNVEPVSPTTKIPETVFTTEMHMSNMSNVHE 447

Query: 442 -------LLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTMD 466
                  ++S  +L  GV  +R+GLW FDL V Q +Q+ V ES+R ++ G QN++   +D
Sbjct: 448 MSTKPIPIVSVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMNYLLD 507

BLAST of CmoCh03G003270 vs. ExPASy Swiss-Prot
Match: Q9NP59 (Solute carrier family 40 member 1 OS=Homo sapiens OX=9606 GN=SLC40A1 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.5e-57
Identity = 160/518 (30.89%), Postives = 254/518 (49.03%), Query Frame = 0

Query: 22  LYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDWVDKLA 81
           LY GH L+ WG RMW F+V ++++ ++ NSLL  A+YG+V + S    G I+GDWVDK A
Sbjct: 28  LYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLLTAVYGLVVAGSVLVLGAIIGDWVDKNA 87

Query: 82  YVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG------FILLVILTNIAGAVG 141
            +KV +  LV QN+S I+ G  ++ +  +   + T + G      +IL++ + NIA    
Sbjct: 88  RLKVAQTSLVVQNVSVILCGIILMMVFLHKHELLTMYHGWVLTSCYILIITIANIAN--- 147

Query: 142 ALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLK 201
            L+S A  I I+R+W+VV++     + L N+N+T+RRID +  + +P+  G I++F S  
Sbjct: 148 -LASTATAITIQRDWIVVVAGEDRSK-LANMNATIRRIDQLTNILAPMAVGQIMTFGSPV 207

Query: 202 ASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRD-----------VEG 261
                ++ WN+VS+  EY L   VY   PAL   +  +     L+            +EG
Sbjct: 208 IGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLKEEETELKQLNLHKDTEPKPLEG 267

Query: 262 S--SSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFT 321
           +    V     +E  H  E +   +M   F  F     W  Y  Q   L G+ LA L+ T
Sbjct: 268 THLMGVKDSNIHELEHEQEPTCASQMAEPFRTFR--DGWVSYYNQPVFLAGMGLAFLYMT 327

Query: 322 VLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQ 381
           VL F  + T     +G+   I+ I  G SA  GI  T+ +  ++ +   +RTGL S  +Q
Sbjct: 328 VLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAFTWLRRKCGLVRTGLISGLAQ 387

Query: 382 WSCLLVCVASIWMQNS-------------------------------------------- 441
            SCL++CV S++M  S                                            
Sbjct: 388 LSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITPTKIPEITTEIYMSNGSNSAN 447

Query: 442 -----------LLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQ 466
                      ++S  +L  GV  +R+GLW FDL V Q +Q+ V ES+R ++ G QN++ 
Sbjct: 448 IVPETSPESVPIISVSLLFAGVIAARIGLWSFDLTVTQLLQENVIESERGIINGVQNSMN 507

BLAST of CmoCh03G003270 vs. ExPASy TrEMBL
Match: A0A6J1GDT9 (Solute carrier family 40 protein OS=Cucurbita moschata OX=3662 GN=LOC111453092 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.5e-278
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV
Sbjct: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE
Sbjct: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KLASCCSRWSPSFNFNHSF
Sbjct: 481 KLASCCSRWSPSFNFNHSF 499

BLAST of CmoCh03G003270 vs. ExPASy TrEMBL
Match: A0A6J1ITB3 (Solute carrier family 40 protein OS=Cucurbita maxima OX=3661 GN=LOC111478216 PE=3 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 7.0e-268
Identity = 482/499 (96.59%), Postives = 486/499 (97.39%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQS  SSSLL SLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV
Sbjct: 1   MENEPLLSPLQSLPSSSLLWSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISE +P EMLTN+NSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISEHYPSEMLTNMNSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGH AETSWKVK+FNWFSKFPFVSAWK+YLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHLAETSWKVKIFNWFSKFPFVSAWKVYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATL YPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLAYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVA SRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAASRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           G QNALQSTMDLMGYVMGIVVSNPQDFWKLILISFT VTLAALLYTVHLYRVRKHLFHLE
Sbjct: 421 GTQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTAVTLAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KL SCCSRWSPSF  NHSF
Sbjct: 481 KLVSCCSRWSPSFTVNHSF 499

BLAST of CmoCh03G003270 vs. ExPASy TrEMBL
Match: A0A0A0KXR0 (Solute carrier family 40 protein OS=Cucumis sativus OX=3659 GN=Csa_4G314520 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 443/506 (87.55%), Postives = 467/506 (92.29%), Query Frame = 0

Query: 1   MENEPLLSPLQS---PASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAI 60
           MENEPLLSP  S   P SSSLLR LY GHFLARWGARMWEFSVGLYMISVWPNSLLFAAI
Sbjct: 1   MENEPLLSPPPSSPLPLSSSLLRCLYIGHFLARWGARMWEFSVGLYMISVWPNSLLFAAI 60

Query: 61  YGVVESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTY 120
           YGVVESASTAFFGPIVGD VDKLAYVKVLKIWL TQNLSYI+AG TVVALLFYSDL S+Y
Sbjct: 61  YGVVESASTAFFGPIVGDLVDKLAYVKVLKIWLATQNLSYIVAGVTVVALLFYSDLKSSY 120

Query: 121 FTGFILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVC 180
           FTGFILLVILTNIAGAVGALSSLAGTIL+EREWVVVISERHPPE+LTNINSTMRRIDLVC
Sbjct: 121 FTGFILLVILTNIAGAVGALSSLAGTILVEREWVVVISERHPPEVLTNINSTMRRIDLVC 180

Query: 181 KLFSPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRL 240
           KL SPVISGFIISF+SLKASA+TLAVWNI+SVW EYWLFTSVYDGIPALEESS RRVSRL
Sbjct: 181 KLLSPVISGFIISFISLKASAMTLAVWNIISVWLEYWLFTSVYDGIPALEESSQRRVSRL 240

Query: 241 VLRDVEGSSSVSR-----FPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLP 300
            LRDV  SSSVS+      P+  D  SAE SWKVKMFNWFSK PFV+AWK+YLEQDTVLP
Sbjct: 241 ALRDVGESSSVSQQIERLIPNDVDARSAERSWKVKMFNWFSKVPFVTAWKVYLEQDTVLP 300

Query: 301 GIALAVLFFTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTL 360
           G+ALA+LFFTVLSFGTLMTATLEWEGIPAYIIGIARG+SATIGIAATLVYPI+QSRI TL
Sbjct: 301 GVALALLFFTVLSFGTLMTATLEWEGIPAYIIGIARGVSATIGIAATLVYPIVQSRILTL 360

Query: 361 RTGLWSIWSQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVP 420
           RTGLWSIWSQW+CLLVCV SIW+QNSLLSAYMLM+GVAVSRLGLWMFDLAVIQQMQDQVP
Sbjct: 361 RTGLWSIWSQWTCLLVCVVSIWIQNSLLSAYMLMMGVAVSRLGLWMFDLAVIQQMQDQVP 420

Query: 421 ESDRCVVGGAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRV 480
           ESDRCVVGGAQNALQSTMDLMGYVMG+++SNPQDFWKLILISFT VTLAALLYT+HLYR+
Sbjct: 421 ESDRCVVGGAQNALQSTMDLMGYVMGVIISNPQDFWKLILISFTAVTLAALLYTIHLYRI 480

Query: 481 RKHLFHLEKLASCCSRWSPSFNFNHS 499
           RKHLFH+EKLASCCSRW  S   N S
Sbjct: 481 RKHLFHMEKLASCCSRWPLSLAINSS 506

BLAST of CmoCh03G003270 vs. ExPASy TrEMBL
Match: A0A1S3CMJ9 (Solute carrier family 40 protein OS=Cucumis melo OX=3656 GN=LOC103502175 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.3e-241
Identity = 439/506 (86.76%), Postives = 462/506 (91.30%), Query Frame = 0

Query: 1   MENEPLLSPLQS---PASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAI 60
           M  EPLLSP  S   P SSSLLR LY GHFLARWGARMWEFSVGLYMISVWPNSLLFAAI
Sbjct: 1   MVKEPLLSPPPSSLLPLSSSLLRCLYIGHFLARWGARMWEFSVGLYMISVWPNSLLFAAI 60

Query: 61  YGVVESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTY 120
           YGVVESASTAFFGPIVGD VDKLAYVKVLKIWL TQN+SYIIAG TVVALLFYSDL STY
Sbjct: 61  YGVVESASTAFFGPIVGDLVDKLAYVKVLKIWLATQNISYIIAGVTVVALLFYSDLKSTY 120

Query: 121 FTGFILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVC 180
           FTGFILLVILTNI GAVGALSSLAGTILIEREWVVVISER PPE+LTNINS MRRIDLVC
Sbjct: 121 FTGFILLVILTNITGAVGALSSLAGTILIEREWVVVISERRPPEVLTNINSMMRRIDLVC 180

Query: 181 KLFSPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRL 240
           KL SPVISGFIISF+SLKASA+TLAVWNI+SVW EYWLFTSVYDGIPALEESS RRVSRL
Sbjct: 181 KLLSPVISGFIISFISLKASAMTLAVWNIISVWLEYWLFTSVYDGIPALEESSQRRVSRL 240

Query: 241 VLRDVEGSSSVSR-----FPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLP 300
            LRD+  SSSVS+      P+  D  SAE SWKVK+FNWFSK PFV AWK+YLEQDTVLP
Sbjct: 241 ALRDMGESSSVSQQIERLLPNDVDARSAERSWKVKVFNWFSKVPFVRAWKVYLEQDTVLP 300

Query: 301 GIALAVLFFTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTL 360
           G+ALA+LFFTVLSFGTLMTATLEWEGIPAYIIGIARG+SATIGIAATLVYPI+QSRI TL
Sbjct: 301 GVALALLFFTVLSFGTLMTATLEWEGIPAYIIGIARGVSATIGIAATLVYPIVQSRILTL 360

Query: 361 RTGLWSIWSQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVP 420
           RTGLWSIWSQW+CLLVCV SIW+QNSLLSAYMLM+GVAVSRLGLWMFDLAVIQQMQDQVP
Sbjct: 361 RTGLWSIWSQWTCLLVCVVSIWIQNSLLSAYMLMMGVAVSRLGLWMFDLAVIQQMQDQVP 420

Query: 421 ESDRCVVGGAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRV 480
           ESDRCVVGGAQNALQSTMDLMGYVMG+V+SNPQDFWKL+LISFT VTLAALLYTVHLYR+
Sbjct: 421 ESDRCVVGGAQNALQSTMDLMGYVMGVVISNPQDFWKLVLISFTAVTLAALLYTVHLYRI 480

Query: 481 RKHLFHLEKLASCCSRWSPSFNFNHS 499
           RKHLFH+EKLASCCSRW  SF  + S
Sbjct: 481 RKHLFHMEKLASCCSRWPLSFTVHSS 506

BLAST of CmoCh03G003270 vs. ExPASy TrEMBL
Match: A0A6J1CSL5 (Solute carrier family 40 protein OS=Momordica charantia OX=3673 GN=LOC111013840 PE=3 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.6e-238
Identity = 433/510 (84.90%), Postives = 461/510 (90.39%), Query Frame = 0

Query: 1   MENEPLLSPLQSPAS--------SSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSL 60
           ME E +LSP + P S        SSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSL
Sbjct: 1   MEKETILSPEEYPPSSSSENPLPSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSL 60

Query: 61  LFAAIYGVVESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSD 120
           LFAAIYGVVESASTAF GP+VG+WVDKLAYVKVLK+WLVTQNLSYIIAG TVVALLFYSD
Sbjct: 61  LFAAIYGVVESASTAFLGPVVGEWVDKLAYVKVLKVWLVTQNLSYIIAGVTVVALLFYSD 120

Query: 121 LISTYFTGFILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRR 180
           L S YFTGFILLVILTN++GAVGALSSLAGTILIEREWVVVISE H PE+LTNINST+RR
Sbjct: 121 LRSIYFTGFILLVILTNVSGAVGALSSLAGTILIEREWVVVISEGHSPEVLTNINSTIRR 180

Query: 181 IDLVCKLFSPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHR 240
           IDLVCKL SPVISGFIISF+SLKASA+TLAVWNI+SVW EYWLF SVYDGIPALEESS R
Sbjct: 181 IDLVCKLLSPVISGFIISFISLKASALTLAVWNIISVWLEYWLFISVYDGIPALEESSRR 240

Query: 241 RVSRLVLRDVEGSSSV-----SRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQ 300
           RVSRLVLRDVE SSS      S  P+ EDG SAE  WK KM +W + FPF SAWK+YL+Q
Sbjct: 241 RVSRLVLRDVEESSSAPQQIESLLPNDEDGRSAERKWKTKMIDWIAMFPFTSAWKVYLQQ 300

Query: 301 DTVLPGIALAVLFFTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQS 360
           DTVLPGIALAVLFFTVLSFGTLMTATLEWEG+PAYIIG+ARGISATIGIAATLVYPI+QS
Sbjct: 301 DTVLPGIALAVLFFTVLSFGTLMTATLEWEGMPAYIIGLARGISATIGIAATLVYPIVQS 360

Query: 361 RISTLRTGLWSIWSQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQM 420
           RISTLRTGLWSIWSQW+CL+VCVASIW+QNSL SAYMLMIGVA SRLGLWMFDLA+IQQM
Sbjct: 361 RISTLRTGLWSIWSQWTCLVVCVASIWVQNSLWSAYMLMIGVATSRLGLWMFDLAIIQQM 420

Query: 421 QDQVPESDRCVVGGAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTV 480
           QDQV ESDRCVVGGAQNALQS+MDLMGYVMGIVVSNPQDFWKLIL+SF  VTLAALLYT+
Sbjct: 421 QDQVAESDRCVVGGAQNALQSSMDLMGYVMGIVVSNPQDFWKLILVSFAAVTLAALLYTI 480

Query: 481 HLYRVRKHLFHLEKLASCCSRWSP-SFNFN 497
           HLYR+RKHLFHLEKLASCCSRWSP SF  N
Sbjct: 481 HLYRIRKHLFHLEKLASCCSRWSPISFTVN 510

BLAST of CmoCh03G003270 vs. NCBI nr
Match: XP_022949805.1 (solute carrier family 40 member 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 968.0 bits (2501), Expect = 3.1e-278
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV
Sbjct: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE
Sbjct: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KLASCCSRWSPSFNFNHSF
Sbjct: 481 KLASCCSRWSPSFNFNHSF 499

BLAST of CmoCh03G003270 vs. NCBI nr
Match: KAG7033743.1 (Solute carrier family 40 member 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 963.0 bits (2488), Expect = 1.0e-276
Identity = 496/499 (99.40%), Postives = 497/499 (99.60%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQSP SSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV
Sbjct: 1   MENEPLLSPLQSPPSSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWK+YLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKVYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVT AALLYTVHLYRVRKHLFHLE
Sbjct: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTFAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KLASCCSRWSPSFNFNHSF
Sbjct: 481 KLASCCSRWSPSFNFNHSF 499

BLAST of CmoCh03G003270 vs. NCBI nr
Match: XP_023543301.1 (solute carrier family 40 member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.4 bits (2445), Expect = 9.7e-272
Identity = 489/499 (98.00%), Postives = 491/499 (98.40%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQSP SSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAA+YGV
Sbjct: 1   MENEPLLSPLQSPPSSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAVYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRF DYEDGHSAETSWKVKMFNWFSKFPFVSAWK+YLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFLDYEDGHSAETSWKVKMFNWFSKFPFVSAWKVYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATL YPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLAYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVA SRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAASRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFT VTLAALLYTVHLYRVRKHLFHLE
Sbjct: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTAVTLAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KLASC SRWSPSF  NHSF
Sbjct: 481 KLASCFSRWSPSFTVNHSF 499

BLAST of CmoCh03G003270 vs. NCBI nr
Match: XP_022978149.1 (solute carrier family 40 member 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 932.6 bits (2409), Expect = 1.5e-267
Identity = 482/499 (96.59%), Postives = 486/499 (97.39%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQS  SSSLL SLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV
Sbjct: 1   MENEPLLSPLQSLPSSSLLWSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISE +P EMLTN+NSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISEHYPSEMLTNMNSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGH AETSWKVK+FNWFSKFPFVSAWK+YLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHLAETSWKVKIFNWFSKFPFVSAWKVYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATL YPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLAYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVA SRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAASRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           G QNALQSTMDLMGYVMGIVVSNPQDFWKLILISFT VTLAALLYTVHLYRVRKHLFHLE
Sbjct: 421 GTQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTAVTLAALLYTVHLYRVRKHLFHLE 480

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KL SCCSRWSPSF  NHSF
Sbjct: 481 KLVSCCSRWSPSFTVNHSF 499

BLAST of CmoCh03G003270 vs. NCBI nr
Match: KAG6603553.1 (Solute carrier family 40 member 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 879.0 bits (2270), Expect = 1.9e-251
Identity = 465/499 (93.19%), Postives = 468/499 (93.79%), Query Frame = 0

Query: 1   MENEPLLSPLQSPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGV 60
           MENEPLLSPLQS  SSSLLRSL                       + WP+  L  AIYGV
Sbjct: 1   MENEPLLSPLQSLPSSSLLRSLTPD--------------------TSWPDGAL--AIYGV 60

Query: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120
           VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG
Sbjct: 61  VESASTAFFGPIVGDWVDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTG 120

Query: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180
           FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF
Sbjct: 121 FILLVILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLF 180

Query: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240
           SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR
Sbjct: 181 SPVISGFIISFVSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLR 240

Query: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLF 300
           DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWK+YLEQDTVLPGIALAVLF
Sbjct: 241 DVEGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKVYLEQDTVLPGIALAVLF 300

Query: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360
           FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW
Sbjct: 301 FTVLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIW 360

Query: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420
           SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG
Sbjct: 361 SQWSCLLVCVASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVG 420

Query: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLE 480
           GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVT AALLYTVHLYRVRKHLFHLE
Sbjct: 421 GAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTFAALLYTVHLYRVRKHLFHLE 477

Query: 481 KLASCCSRWSPSFNFNHSF 500
           KLASCCSRWSPSFNFNHSF
Sbjct: 481 KLASCCSRWSPSFNFNHSF 477

BLAST of CmoCh03G003270 vs. TAIR 10
Match: AT2G38460.1 (iron regulated 1 )

HSP 1 Score: 599.7 bits (1545), Expect = 2.1e-171
Identity = 306/480 (63.75%), Postives = 372/480 (77.50%), Query Frame = 0

Query: 22  LYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDWVDKLA 81
           LY G+FLARW AR WEFSV LYMI +WPNSLL AAIYG +ES STA FGPIVG WV+ + 
Sbjct: 41  LYVGYFLARWSARTWEFSVALYMIHLWPNSLLLAAIYGAIESGSTAIFGPIVGQWVEGMD 100

Query: 82  YVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGALSSLA 141
           YVKVL++WL+ QNLSY IAG  V+ LL  SDL S     F +L++LTN+AGA+G LS+LA
Sbjct: 101 YVKVLRLWLLFQNLSYTIAGGAVIKLLLVSDLKSRNLPVFAILIVLTNLAGAIGVLSTLA 160

Query: 142 GTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKASAVTL 201
           GTILIER+W VV+SE HPP +LT +NS +R IDL  KL SPVI+G IISFVSLKASA+T 
Sbjct: 161 GTILIERDWAVVMSEGHPPAVLTKMNSVIRGIDLSSKLLSPVITGLIISFVSLKASAITF 220

Query: 202 AVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSS---SVSRFPDYEDGH 261
           A W  ++ W EYWLF SVY G+PA+  S+ RR+ R   + VEG     SVS  P  E+G+
Sbjct: 221 AAWATITAWVEYWLFISVYSGVPAITRSNERRILRSRTKQVEGRDAPVSVSIVPGTEEGY 280

Query: 262 SAETSWK---VKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLMTATLE 321
           +     +   + + +  SK  FV AW++Y  Q+ VLPG++LA+LFFTVLSFGTLMTATL+
Sbjct: 281 TGNPPSRTGILVILDRMSKSSFVGAWRIYFNQEVVLPGVSLALLFFTVLSFGTLMTATLQ 340

Query: 322 WEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCVASIWM 381
           WEGIP YIIGI RGISAT+G+AATLVYP++QSR+STLRTGLWS WSQWSCLLVCV SIW+
Sbjct: 341 WEGIPTYIIGIGRGISATVGLAATLVYPLMQSRLSTLRTGLWSFWSQWSCLLVCVGSIWV 400

Query: 382 QNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTMDLMGY 441
           +   +++YMLM GVA SRLGLWMFDLAVIQQMQD V ESDRCVVGG QN+LQS +DLM Y
Sbjct: 401 KKDKIASYMLMAGVAASRLGLWMFDLAVIQQMQDLVSESDRCVVGGVQNSLQSALDLMAY 460

Query: 442 VMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEK---LASCCSRWSPS 493
           ++GI+VSNP+DFW L LISF+ V+LA +LYT+HLYR+R H+FHLEK   L  C  +  PS
Sbjct: 461 LLGIIVSNPKDFWILTLISFSTVSLAGMLYTIHLYRIRNHIFHLEKIPLLNKCIFKLLPS 520

BLAST of CmoCh03G003270 vs. TAIR 10
Match: AT5G03570.1 (iron regulated 2 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.2e-166
Identity = 297/472 (62.92%), Postives = 369/472 (78.18%), Query Frame = 0

Query: 17  SLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDW 76
           S++ SLY G+FLARWGAR WEFSV LYMI +WPNSL   A+YGVVES S   FGPIVG  
Sbjct: 32  SMVISLYLGYFLARWGARTWEFSVALYMIYLWPNSLFLTAMYGVVESGSATLFGPIVGQM 91

Query: 77  VDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGA 136
           +D ++YVKVL++WLVTQNLS+I+AG  VVALL   DL S  F  F  LV+LTN++GA+G 
Sbjct: 92  IDGMSYVKVLRLWLVTQNLSFIVAGGAVVALLVVPDLKSQNFPVFATLVVLTNLSGAIGV 151

Query: 137 LSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKA 196
           LS+LAGT+LIER+WVVV+SE H P +LT +NS +R IDL  KL SPVI+G IISFVSL+A
Sbjct: 152 LSTLAGTVLIERDWVVVMSEGHSPAVLTRMNSVIRGIDLSSKLLSPVITGLIISFVSLRA 211

Query: 197 SAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSSSVSRF----- 256
           SA+T A W  ++VW EYWLF SVY+G+PA+ +S  RR  R      E + S S F     
Sbjct: 212 SAITFAAWATITVWIEYWLFISVYNGVPAIVQSDERRSLRSSQSQAEETDSASSFYVPLL 271

Query: 257 PDYEDGHSAETSWKV-KMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLM 316
            + E   + ++  ++ ++    S+  FVSAW+ YL Q+ VLPG++LA+LFFTVLSFGTLM
Sbjct: 272 HEEESYRNTQSRSRILRILERISESSFVSAWRNYLNQEIVLPGVSLALLFFTVLSFGTLM 331

Query: 317 TATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCV 376
           TATLEW+GIP YIIGI RGISA +G+AAT++YP++QSRIS LRTG+WS WSQW+CLLVCV
Sbjct: 332 TATLEWKGIPTYIIGIGRGISAGVGLAATVLYPLMQSRISPLRTGVWSFWSQWTCLLVCV 391

Query: 377 ASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTM 436
            SIW++   +++YMLM GVA SRLGLWMFDLAVIQQMQD VPESDRCVVGG QN+LQS +
Sbjct: 392 GSIWVEKEKIASYMLMAGVAASRLGLWMFDLAVIQQMQDLVPESDRCVVGGVQNSLQSAL 451

Query: 437 DLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEKL 483
           DLM  ++GI+VSNP+DFW L LISF  V+LA +LYT+HLYR+RKHLFHLEK+
Sbjct: 452 DLMANLLGIIVSNPKDFWMLTLISFATVSLAGILYTIHLYRIRKHLFHLEKI 503

BLAST of CmoCh03G003270 vs. TAIR 10
Match: AT5G03570.2 (iron regulated 2 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.2e-166
Identity = 297/472 (62.92%), Postives = 369/472 (78.18%), Query Frame = 0

Query: 17  SLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGPIVGDW 76
           S++ SLY G+FLARWGAR WEFSV LYMI +WPNSL   A+YGVVES S   FGPIVG  
Sbjct: 32  SMVISLYLGYFLARWGARTWEFSVALYMIYLWPNSLFLTAMYGVVESGSATLFGPIVGQM 91

Query: 77  VDKLAYVKVLKIWLVTQNLSYIIAGATVVALLFYSDLISTYFTGFILLVILTNIAGAVGA 136
           +D ++YVKVL++WLVTQNLS+I+AG  VVALL   DL S  F  F  LV+LTN++GA+G 
Sbjct: 92  IDGMSYVKVLRLWLVTQNLSFIVAGGAVVALLVVPDLKSQNFPVFATLVVLTNLSGAIGV 151

Query: 137 LSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVISGFIISFVSLKA 196
           LS+LAGT+LIER+WVVV+SE H P +LT +NS +R IDL  KL SPVI+G IISFVSL+A
Sbjct: 152 LSTLAGTVLIERDWVVVMSEGHSPAVLTRMNSVIRGIDLSSKLLSPVITGLIISFVSLRA 211

Query: 197 SAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDVEGSSSVSRF----- 256
           SA+T A W  ++VW EYWLF SVY+G+PA+ +S  RR  R      E + S S F     
Sbjct: 212 SAITFAAWATITVWIEYWLFISVYNGVPAIVQSDERRSLRSSQSQAEETDSASSFYVPLL 271

Query: 257 PDYEDGHSAETSWKV-KMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFTVLSFGTLM 316
            + E   + ++  ++ ++    S+  FVSAW+ YL Q+ VLPG++LA+LFFTVLSFGTLM
Sbjct: 272 HEEESYRNTQSRSRILRILERISESSFVSAWRNYLNQEIVLPGVSLALLFFTVLSFGTLM 331

Query: 317 TATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWSQWSCLLVCV 376
           TATLEW+GIP YIIGI RGISA +G+AAT++YP++QSRIS LRTG+WS WSQW+CLLVCV
Sbjct: 332 TATLEWKGIPTYIIGIGRGISAGVGLAATVLYPLMQSRISPLRTGVWSFWSQWTCLLVCV 391

Query: 377 ASIWMQNSLLSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCVVGGAQNALQSTM 436
            SIW++   +++YMLM GVA SRLGLWMFDLAVIQQMQD VPESDRCVVGG QN+LQS +
Sbjct: 392 GSIWVEKEKIASYMLMAGVAASRLGLWMFDLAVIQQMQDLVPESDRCVVGGVQNSLQSAL 451

Query: 437 DLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLYTVHLYRVRKHLFHLEKL 483
           DLM  ++GI+VSNP+DFW L LISF  V+LA +LYT+HLYR+RKHLFHLEK+
Sbjct: 452 DLMANLLGIIVSNPKDFWMLTLISFATVSLAGILYTIHLYRIRKHLFHLEKI 503

BLAST of CmoCh03G003270 vs. TAIR 10
Match: AT5G26820.1 (iron-regulated protein 3 )

HSP 1 Score: 103.2 bits (256), Expect = 6.1e-22
Identity = 121/467 (25.91%), Postives = 215/467 (46.04%), Query Frame = 0

Query: 12  SPASSSLLRSLYAGHFLARWGARMWEFSVGLYMISVWPNSLLFAAIYGVVESASTAFFGP 71
           +PA    L  LYA   +     ++W F+    +  ++P SLL  A+ G V   +    GP
Sbjct: 142 TPAHPEGLYVLYASCLVGNLVEQLWNFAWPSAIAMLYP-SLLPVAVMGFVTKLAIIAGGP 201

Query: 72  IVGDWVDKL----AYVKVLKIWLVTQNLS--YIIAGATVVALLFYSDLISTYFTGFILLV 131
           +VG ++D       Y+ +  I    Q LS   II   TV +    S L+  +F  F LL 
Sbjct: 202 VVGKFMDYSPRVPTYISLNVIQAAAQVLSAGMIIHAYTVPSTSASSILLQPWF--FALL- 261

Query: 132 ILTNIAGAVGALSSLAGTILIEREWVVVISERHPPEMLTNINSTMRRIDLVCKLFSPVIS 191
                AGA+ +L  +A  + IER+WVV+++  + P  L   N+ + RIDL+C++   ++ 
Sbjct: 262 ----FAGAIDSLCGIASGVAIERDWVVLLAGINRPIALAQANAVLHRIDLLCEIAGTMLF 321

Query: 192 GFIISF---VSLKASAVTLAVWNIVSVWFEYWLFTSVYDGIPALEESSHRRVSRLVLRDV 251
           G ++S    V+    A TL V ++ ++    WL      G+    + S    S       
Sbjct: 322 GILLSKYDPVTCLKFAATLMVGSLPTMTALIWLTNKFSSGVLDRPKCSLNSCS------A 381

Query: 252 EGSSSVSRFPDYEDGHSAETSWKVKMFNWFSKFPFVSAWKMYLEQDTVLPGIALAVLFFT 311
           EGS +           + ++ + + M            WK Y++Q  +   +A  +L+F 
Sbjct: 382 EGSRT-----------NTDSIFDIGMET------IKLGWKEYIQQPVLPASLAYVLLYFN 441

Query: 312 -VLSFGTLMTATLEWEGIPAYIIGIARGISATIGIAATLVYPIIQSRISTLRTGLWSIWS 371
            VL+ G+LMTA L    +   +IG   G+ A +G+AAT +   +  R+  L+ G   ++ 
Sbjct: 442 IVLTPGSLMTAFLTQRCVNPSVIGGFSGLCAVMGVAATFLSANLVKRVGILKAGAVGLFF 501

Query: 372 QWSCLLVCVASIWMQNSL---LSAYMLMIGVAVSRLGLWMFDLAVIQQMQDQVPESDRCV 431
           Q S L V VA ++  +SL      +  +  + +SRLG   + +   Q +Q  +P S   +
Sbjct: 502 QASLLAVAVA-VYCSSSLSHKSPLFFFLSMIVLSRLGHMSYGVVGAQILQTGIPSSKANL 561

Query: 432 VGGAQNALQSTMDLMGYVMGIVVSNPQDFWKLILISFTVVTLAALLY 466
           +G  + ++ S  + +   + I  ++   F  L ++S   V  A+L++
Sbjct: 562 IGATEISVASLAESLMLGVAIAANDASHFGFLAVLSLLSVVAASLIF 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O809052.9e-17063.75Solute carrier family 40 member 1 OS=Arabidopsis thaliana OX=3702 GN=IREG1 PE=3 ... [more]
F4KGN51.7e-16562.92Solute carrier family 40 member 2 OS=Arabidopsis thaliana OX=3702 GN=IREG2 PE=2 ... [more]
Q5Z9221.7e-15459.88Solute carrier family 40 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
Q9JHI96.7e-5830.74Solute carrier family 40 member 1 OS=Mus musculus OX=10090 GN=Slc40a1 PE=1 SV=1[more]
Q9NP591.5e-5730.89Solute carrier family 40 member 1 OS=Homo sapiens OX=9606 GN=SLC40A1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GDT91.5e-278100.00Solute carrier family 40 protein OS=Cucurbita moschata OX=3662 GN=LOC111453092 P... [more]
A0A6J1ITB37.0e-26896.59Solute carrier family 40 protein OS=Cucurbita maxima OX=3661 GN=LOC111478216 PE=... [more]
A0A0A0KXR01.1e-24487.55Solute carrier family 40 protein OS=Cucumis sativus OX=3659 GN=Csa_4G314520 PE=3... [more]
A0A1S3CMJ97.3e-24186.76Solute carrier family 40 protein OS=Cucumis melo OX=3656 GN=LOC103502175 PE=3 SV... [more]
A0A6J1CSL57.6e-23884.90Solute carrier family 40 protein OS=Momordica charantia OX=3673 GN=LOC111013840 ... [more]
Match NameE-valueIdentityDescription
XP_022949805.13.1e-278100.00solute carrier family 40 member 1-like isoform X1 [Cucurbita moschata][more]
KAG7033743.11.0e-27699.40Solute carrier family 40 member 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023543301.19.7e-27298.00solute carrier family 40 member 1-like [Cucurbita pepo subsp. pepo][more]
XP_022978149.11.5e-26796.59solute carrier family 40 member 1-like isoform X1 [Cucurbita maxima][more]
KAG6603553.11.9e-25193.19Solute carrier family 40 member 1, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G38460.12.1e-17163.75iron regulated 1 [more]
AT5G03570.11.2e-16662.92iron regulated 2 [more]
AT5G03570.21.2e-16662.92iron regulated 2 [more]
AT5G26820.16.1e-2225.91iron-regulated protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009716Ferroportin-1PFAMPF06963FPN1coord: 18..458
e-value: 4.0E-159
score: 529.8
IPR009716Ferroportin-1PANTHERPTHR11660SOLUTE CARRIER FAMILY 40 MEMBERcoord: 17..483
IPR009716Ferroportin-1CDDcd17480MFS_SLC40A1_likecoord: 22..468
e-value: 1.31211E-134
score: 392.732
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 12..468
e-value: 1.1E-10
score: 42.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 19..476
NoneNo IPR availablePANTHERPTHR11660:SF68SOLUTE CARRIER FAMILY 40 MEMBER 1coord: 17..483

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G003270.1CmoCh03G003270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034755 iron ion transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005381 iron ion transmembrane transporter activity