CmoCh03G002030 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G002030
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	NADH--cytochrome b5 reductase 1-like
Location	Cmo_Chr03: 3329411 .. 3335016 (-)
RNA-Seq Expression	CmoCh03G002030
Synteny	CmoCh03G002030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTAGAAGTTAATTTCTTCCCCCTTGGTTGGTTATTCAAGAAAAATTGTGCATCACCGTCGATGGATTTAGCTCACCCATTTGTTTTTCATTAGTTGGAGGACTTCCAAACACCTGCCTCAGGCTTCACCTAATTCGGCGTCACTTCATTTTTCTTCAGTGGCTGTGGTGGCTGTGACGGCTGTGGCTGGGGTTGGTGAAAGCGGGTGTCGCGGCGTGGGGCCGGAAAAAGTTAGGTGAATCGTAATCTATTCTAAAAATCCTTTAAATTCCACTCCAAATATCGAAAACCCATTTGATTTCTCTCGAGGTTTTTTTCTGGGAAGAATCGGTGATGGATTTCTTACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTTCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGGTTAGCTACTCTTGAATCTCAGTTTTTCTGTAGTTCCCATTGCTTTGATTTCTGGGATCGGTTGTGTTTTGATTTGTTCTCTTTTATGAAAATTTGAGATTCCGGTGAAGTTCTTACATGTCACTTCGTGAATAAGTGGAGTTTTTGAGTTAGAAATCGATATGGATTAAGGATTGGATCCATATCTGTTATTATTTTGTTTGTTGTTATATTATCTGGTTAATCTCTTCGTATCAACTCATAGTTTAAATCTTATTTGTGAAAATTACATATAAAGAGGAGATAAATTGAACTCACTACCCATTAAGTGGATCATATGCTGTCTACTTGAGCTTGAACTATGTTCAAGTGAGCTAGCTTATAGTAACTTGAACTTTCTTGAAGTTGTAGAAGCTAATTAATCTATGTTACATGGTTCCATTTTCTGTCTAGATCTGTAATGGACATTCCAGTTCAAATTCGACCGATGATTTGTTTTAAATTGATATCAGTTAAGGAGATTTGATGGGAAACATCACATAAACAGGGCTTCATTTTAGAGTTGTCCACGAAAATATTATATTGTTCGATAATAAGAAGGGTCGATTCAGTAATGTGTGGATCAAATGTGGTGTTCACTCAACCCTTTAGCAGGATTCTAGTCCTTTGGGTGGTTTTGTTGTGATGATACATTAGAACATTGTCTGTTATTGGTGCTTTATTTGATTCATTTTCTTAATTTGTTTACTGTATGATTGGAAAGACGTGCTCGGATGTTCTTGGGTAAGCACGAAAGCATCCTTACTTTTATAGAAGCTGCATCCTTTCTAGTTTTATCTTGGAGTGAATTATCTTATCCATTATGTACTTACAACTTTTCTACCCTCCTATAGGACATTCTTTTGTAATCGATTTCATCGATTAATGAAGTGGTTTCTGTTTCCTCGAAATTTAAGTTGTTTTCCTTATGATATGGTTTTGTGCATTCAGGTTGCTTGGATCCAGAGATATTCAAGGAGTTTAAACTTATTAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCCAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGTCTAGTAGTTGATCCTTATTTATTCTTCATAGGATTATTTGGATTTCTACTCACCATGGCTATGGATACGTAGCTGATTGAAAAAACAATGTTGATTTTGGCACTATATGGTGTAGGAATCACGATACTATACTCTCCTGTGTTAACTCGGTAACACTTAAGAGAGATATCCTGAAATAAGCAAGTTTCCTTTAAAGATACCTGTTAAACTGTAACGACCCTAGCCTACCGCTAGCAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTTCTCTCAAGGTTTTTAAAATGTGTTTGGTAGGGGAGAGGTTTCCACACCCTTATAAAGGGTATTTTGTTCTCTTCCCCAACCGATGTGAGATCTCACAATCCACTCTCCTTCGAAGCCCAGTGTCCTTGCTGGCACACCGCCTCGTGTCCACCCCCCTTCGGGGCTCAGCCTCCTCGCTCGCAATGTCGTTTGGTGTCTGGCTTTGATACCAACTGTAACGGCCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCAGGCTTCCCTTCAAGGTTTTTAAAATGCGTCTGCGAGGGAGAAGTTTCCACACCCTTATATAGAATGCTTCGTTCTCCTCTCCAACCTATGTGGGATCTCACATAAACTGTCAATCCTGCCTCTGCTTCATTATAGATTTTTTGATTCAGCTGCCGGTCACAACTTTTGTTGGATAAGGTCACTTGACATTATATTATTTAGGCTACGACTGGTCTTTTCGCCTGAGAGATCTTGTAGGACTTCAAAGAAAACTTCTAAAACTTTGTTACGGTGGAGTATCTTGTGTCTCTTAGCTGCTTGATTGTGTTCATCGTTGAGAAACTTTTCTATTCTTTAAATACGCTAACCAGAAATGATTTCTGGACTCTCCCTTCAGTTTGAGTGGGGATGCGTGCCTTATTGCCCTCTTCACCTGGTGTTGTTTTCGAAGATGACTGTTCCATATTGAATCATTTACTGTCCCTGAGAAATTATAATTCATTCATCTTACAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTGGACTCTGATGTTGGATATTTTGAGCTCGTCATAAAGGTTTGTGTTTTCTGATTCTTAGTTGATGAGCAGTATTTTAATGGTATGCATGCTTGTTAATCTGAACTCTGTAATGTGTTTAATCAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGTATGTTCAATCAATAATGACAGCCTTTTAAGTCATGTTTTTTATGCTTTTAAGTATTGATGTTTTTTGGGATTCGAGCATTCAGCTTCCTTCAGGTTCGGCTGCAGACTTAATGAAACATTTTGTTTGAAGAATTTAATGAAACATTTTGTTTGTCTCAGACACAAAGACCCCTCTTATTCTTTCTCTAGTGAGAGAGAAACAAAGAACTTTATGGTACCTTTGAAAACACTGAGAATATTCAAAATTCACAACGAAAATATTTCTGCAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTACGAATTTCCTTTTCTTTTGGCTTGATCATTTTAGTTCCTCAACTTACCATCATTCGGACGACTTGATTGTTGTTATCGATACTTTGCTTTCTTTTCTGCTCCTTAGGTTGCTCGAGCAATATTAGAAAATCCAAATGACGAGACGAAAGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTTTTAAAGGTAATGTTGTCATCTTTCTTCAATAAACTTACCCCTCGTCCTCGTATTGACCATAAATTTGGATACCAGTTGATCTTCTCATTTCGAAACATGTTGATGGTTAGTTTTTCTCTTGATGGAGAGAGATAAACTAGGCTAACTGGTGTGACCGATGCTCAAGTAGTATCGTAATCAATCTACTCCTTTCTTAGTGCTATGAATTTCAACTGCCGAGTCATTTGATGAAAATGTTTTCGGTACTTTCATGTTTGAACTCCTTAGCTGAACACGTGTATGGAGATCGTTTATCAACTTAAGTATAGTTTAATTAGTCAAGGTATATATCCTCGATCGTAAAGTCATGGTTTGTCATGTGCGATTTTGGGTATTGAAGAGTGAAGGATCTTAATATTTTTGTAGCCTCTTCTTGGGCTTCCACTTTTGCTTTCATAATAATAATTCAATTTCTATGATTTGTGTATCTCTCTATAATTTCATCACTTTTCCTTACCAAAAGAAACTTGTGTGGTTGTAGGAGGAGTTGGATCTTCTTGCCAAAAGTTACCCAGATAGCTTCAAGCTCTATTATGTCTTAAACCAGGTCAGTCTTCTCCTTCACTCACAAATTAATCCTTTTCTCTGTGGTTAAACTTTTTACCATTACAAGATCTCGATGTCAAATGACGGGAAATCGTATTATGCTTATTTTGTTTTGTTGTCTAGACCAATATTAAGTGATCGATATGAACTTCGACGAATCTAGTGAATTAAACTGATTGGTATACTATGTGATTAGATCTCTTTCTTCAAATCTGACCGTTCTTAAAAGTTATCATAGCTCAATCTTGTTCTATTGGCTTGAAGTCCATTTTTTTGTAGCTAGGGTCGAAGATTGTTCGTAAAAGTTAAAACATAGATGAAATTACTTTCAGTCGACCGTTCTCGGAAAGTTGTTTTGTTCTTGTGTGTGCTCTAGAGTCCCCTGAGCACGATTCTTAGAAGTATCTTTAAAAATCGTTATGCAAAACGAGCTATTGAACACTTTCGGATTCGTAAACTTGTGCATTGTGGATGAAATTGGGTTCTCTAAGACCATGATGGATCTACTTGGGAGCTTCATTTATGCTTGCTTGATTATGTACTTTGTTTAATGGTTTGCAATGTCATATCTTTTAATCAACCATTCATATATCAATTGCTTTCTCAGCCTCCTGAAGAATGGGATGGTGGCGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTATATACGATTACGACTATGGTTTCAAATAATATCAATGCTTTGGATCTGTTTCCGAAACCATGTTTGAACGATTTCTTTTTGTCCGAAGTCTTCTCTCGTTTTCTTGTAATTTATTCTTACGAACGACCTGTTTCCAAAAGCGAACGAAGATGTTCTTTCATGTGCACAATAAGTTGGATCAGAGTAGGACCGTTGGATCATTCACTTGTCCGTATTAATAGGTTTAAAAATTAAGCTTATAAACACTACCTTCACCATTCGCTACGCATTTACTCGTTGGATCATTCGCTGCGCATTTATGAGATTGGTAGGATTGTTTGCTGCTGTCTGATCTTTTATTCATGAAACAACAAAATTTTAAGTGTTACCCTGTTTTGTAAGCAGGTTTTAAGATGTGGGCCACCGCCAATGAATAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGAGCCTTCCTCAACCTCCTCACTAGAGCTAAGCATCATTTATTTTTCTTCCCCTTTCTAAATTTTGAACATTATGTACTGAGAGGATGTTGCCTTAGCTCTTTTCACTTTGCAATGTTGAACTTTGATGCCATCAATAACTTCCAACTGTTAGAGCTAGTGAGAGTCTAGACTAGTTTTCATTCACATTGTTTGTTCATGTAACAGTTCTAGAATGGTTTTCTTTTGAAGTGTAGTAAGACCCGTTTAGATTAGCTTTTTGAGTGCTTAAAAACGCTTTTCTTTGCACCTAAAAGTAAAAGGCCAAGTCTACCGCTAGTAGAGATTATCTGCTTTGGCCCATTACGTACTATTAACTTCACGGTTTTTAAAATGTGTCTATTAGGGAGAGTTTTTCAAACCCTTATAAGGAATGATTCGTTTCCCTCTCCAGCTGATATGAGATCTTAT

mRNA sequence

CTCTAGAAGTTAATTTCTTCCCCCTTGGTTGGTTATTCAAGAAAAATTGTGCATCACCGTCGATGGATTTAGCTCACCCATTTGTTTTTCATTAGTTGGAGGACTTCCAAACACCTGCCTCAGGCTTCACCTAATTCGGCGTCACTTCATTTTTCTTCAGTGGCTGTGGTGGCTGTGACGGCTGTGGCTGGGGTTGGTGAAAGCGGGTGTCGCGGCGTGGGGCCGGAAAAAGTTAGGTGAATCGTAATCTATTCTAAAAATCCTTTAAATTCCACTCCAAATATCGAAAACCCATTTGATTTCTCTCGAGGTTTTTTTCTGGGAAGAATCGGTGATGGATTTCTTACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTTCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGGTTGCTTGGATCCAGAGATATTCAAGGAGTTTAAACTTATTAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCCAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTGGACTCTGATGTTGGATATTTTGAGCTCGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTTGCTCGAGCAATATTAGAAAATCCAAATGACGAGACGAAAGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTTTTAAAGGAGGAGTTGGATCTTCTTGCCAAAAGTTACCCAGATAGCTTCAAGCTCTATTATGTCTTAAACCAGCCTCCTGAAGAATGGGATGGTGGCGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTTTTAAGATGTGGGCCACCGCCAATGAATAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGAGCCTTCCTCAACCTCCTCACTAGAGCTAAGCATCATTTATTTTTCTTCCCCTTTCTAAATTTTGAACATTATGTACTGAGAGGATGTTGCCTTAGCTCTTTTCACTTTGCAATGTTGAACTTTGATGCCATCAATAACTTCCAACTGTTAGAGCTAGTGAGAGTCTAGACTAGTTTTCATTCACATTGTTTGTTCATGTAACAGTTCTAGAATGGTTTTCTTTTGAAGTGTAGTAAGACCCGTTTAGATTAGCTTTTTGAGTGCTTAAAAACGCTTTTCTTTGCACCTAAAAGTAAAAGGCCAAGTCTACCGCTAGTAGAGATTATCTGCTTTGGCCCATTACGTACTATTAACTTCACGGTTTTTAAAATGTGTCTATTAGGGAGAGTTTTTCAAACCCTTATAAGGAATGATTCGTTTCCCTCTCCAGCTGATATGAGATCTTAT

Coding sequence (CDS)

ATGGATTTCTTACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTTCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGGTTGCTTGGATCCAGAGATATTCAAGGAGTTTAAACTTATTAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCCAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTGGACTCTGATGTTGGATATTTTGAGCTCGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTTGCTCGAGCAATATTAGAAAATCCAAATGACGAGACGAAAGTACATCTCATTTATGCCAACGTCACCTATGAGGACATTCTTTTAAAGGAGGAGTTGGATCTTCTTGCCAAAAGTTACCCAGATAGCTTCAAGCTCTATTATGTCTTAAACCAGCCTCCTGAAGAATGGGATGGTGGCGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTTTTAAGATGTGGGCCACCGCCAATGAATAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

Protein sequence

MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF

Homology

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BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 5.7e-131
Identity = 226/280 (80.71%), Postives = 250/280 (89.29%), Query Frame = 0


Query: 2   DFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHN 61
           +FL++LD Q+LLG+ VA +A+  G+ Y L+SSK  K R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSK--KRRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP  TSVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 ETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQT 241
           +TKVHLIYANVTY+DILLKEEL+ L  +YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           HCPAPASDIQ+LRCGPPPMNKAM A+LEALGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 139/278 (50.00%), Postives = 189/278 (67.99%), Query Frame = 0


Query: 17  VAVLALAVGSLYVL---SSSKKSKSRG----------CLDPEIFKEFKLIKRVQLSHNVA 76
           +  +ALA G  YV    S S K K  G           LDP+ +++F+L ++  ++HN  
Sbjct: 11  IGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVNHNTR 70

Query: 77  KFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGR 136
            F F LPN   +LGLPIGQHIS R   G G+EV +PYTPI+ D + GYF+L+IK+Y +G 
Sbjct: 71  IFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVYEKGA 130

Query: 137 MSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDET 196
           MS +   M +GD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP+D+T
Sbjct: 131 MSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNPSDKT 190

Query: 197 KVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHC 256
           ++ L++ N+T EDILLK+ELD LA+ +P  FK+YYVLN PP+ W  GVGFVSKE+I++  
Sbjct: 191 EISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKEIIESRL 250

Query: 257 PAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           P+P+    V+ CGPP MNKAM  HLE +G+    +F F
Sbjct: 251 PSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

[Close]

BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 6.0e-72
Identity = 135/264 (51.14%), Postives = 180/264 (68.18%), Query Frame = 0


Query: 16  AVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHNVAKFTFTLPNPTSV 75
           AVAV A+  G       +K  K    L+P  F+EF L +++ +SHNV  + F LP PT +
Sbjct: 34  AVAVTAILAGLKLFTGGNKPRK---VLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDI 93

Query: 76  LGLPIGQHISCRGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVG 135
           LGLPIGQHIS     +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++G
Sbjct: 94  LGLPIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIG 153

Query: 136 DFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDETKVHLIY 195
           D + V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+
Sbjct: 154 DTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIF 213

Query: 196 ANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASD 255
           ANV  +DILLKEELD+LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD
Sbjct: 214 ANVNPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASD 273

Query: 256 IQVLRCGPPPMNKAMGAHLEALGY 274
           +++L CGPPPM  AM    E+LGY
Sbjct: 274 VKILLCGPPPMISAMKKATESLGY 293

[Close]

BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot
Match: Q1DWN4 (NADH-cytochrome b5 reductase 1 OS=Coccidioides immitis (strain RS) OX=246410 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 3.0e-71
Identity = 137/262 (52.29%), Postives = 178/262 (67.94%), Query Frame = 0


Query: 20  LALAVGSLYVLSSSK--KSKSRGCLDPEIFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLG 79
           +A A     VLS+ K   +K R  L+P  F+ F L  +  +SHNV  + F LP PT +LG
Sbjct: 32  IAYATALAVVLSAWKLFSNKPRKVLNPTEFQNFVLKDKTIVSHNVCIYRFALPRPTDILG 91

Query: 80  LPIGQHISCRGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDF 139
           LPIGQHIS      GQ +E+++ YTPI+ D D GYF+L++K YPQG +S H   +R+GD 
Sbjct: 92  LPIGQHISLAATIPGQSKEIVRSYTPISSDDDAGYFDLLVKSYPQGNISKHLTTLRIGDK 151

Query: 140 LAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILE-----NPNDETKVHLIYAN 199
           + V+GPKG   Y P  VR  GM+AGG+GITPM QV +AI++       ND T++ LI+AN
Sbjct: 152 MKVRGPKGAMVYTPNMVRHIGMIAGGTGITPMLQVIKAIIKGRPRNGGNDTTQIDLIFAN 211

Query: 200 VTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQ 259
           V  +DILLKEELD LAK   D+F++YYVLN PPE+W+GGVGFV+ +MI+   PAPA DI+
Sbjct: 212 VNPDDILLKEELDQLAKE-DDAFRIYYVLNNPPEKWNGGVGFVTPDMIKAKLPAPAGDIK 271

Query: 260 VLRCGPPPMNKAMGAHLEALGY 274
           VL CGPPPM  AM    E+LGY
Sbjct: 272 VLICGPPPMVSAMKKATESLGY 292

[Close]

BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot
Match: Q4PGW7 (NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 3.9e-71
Identity = 129/261 (49.43%), Postives = 181/261 (69.35%), Query Frame = 0


Query: 13  LGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHNVAKFTFTLPNP 72
           L + +A +   +GS+ VL      K +  L+P  +++++L+++ +LS N A + F LP  
Sbjct: 49  LNIVLAFVVGLIGSVVVLLYFDSQKIKPVLNPTQWQQYRLMEKQKLSDNTALYRFKLPRS 108

Query: 73  TSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMR 132
            ++LGLPIGQHIS +   G G+ V++ YTP + D D G+F+LV+K Y QG +S +   M+
Sbjct: 109 NNILGLPIGQHISVQANMG-GKTVVRSYTPTSSDDDHGFFDLVVKSYEQGNVSKYIGSMK 168

Query: 133 VGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANV 192
           +GD L+VKGPKG+ RY PG  R  GM+AGG+G+TP  Q+ RA L+NP D+T++  IYANV
Sbjct: 169 IGDLLSVKGPKGQMRYAPGLSRHIGMIAGGTGLTPCLQIIRAALKNPADKTQIDFIYANV 228

Query: 193 TYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQV 252
              DILLK+ELD LA  + D F++ Y LN+ PE W GGVGFV+KE ++ + P PA+DI+V
Sbjct: 229 KETDILLKDELDELALKHKDQFRISYFLNEAPEGWKGGVGFVTKEALEKNLPKPANDIKV 288

Query: 253 LRCGPPPMNKAMGAHLEALGY 274
           L CGPPPM KAM  HLEALGY
Sbjct: 289 LMCGPPPMIKAMTGHLEALGY 308

[Close]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL
Match: A0A6J1GFP5 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453751 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.0e-160
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL
Match: A0A5A7TLD5 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G001310 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.1e-156
Identity = 272/281 (96.80%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQ+LLGLAVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL
Match: A0A1S3C2D1 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.1e-156
Identity = 272/281 (96.80%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQ+LLGLAVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL
Match: A0A6J1HVM7 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.6e-155
Identity = 273/281 (97.15%), Postives = 276/281 (98.22%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDF QSLDPQ+LLGLAVA LAL VG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH
Sbjct: 1   MDFSQSLDPQILLGLAVAGLALVVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVG+FLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGNFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAM AHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL
Match: A0A0A0L226 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G416480 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 2.9e-154
Identity = 269/281 (95.73%), Postives = 276/281 (98.22%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQ+LLG+AVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLP+PTSVLGLPIGQHISC GKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. NCBI nr
Match: XP_022950742.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 1.6e-159
Identity = 281/281 (100.00%), Postives = 281/281 (100.00%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. NCBI nr
Match: KAG7033625.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570.9 bits (1470), Expect = 6.3e-159
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. NCBI nr
Match: KAG6603441.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 569.3 bits (1466), Expect = 1.8e-158
Identity = 279/281 (99.29%), Postives = 279/281 (99.29%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMR GDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRAGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. NCBI nr
Match: XP_023543481.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.7 bits (1454), Expect = 4.5e-157
Identity = 276/281 (98.22%), Postives = 278/281 (98.93%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MD  QSLDPQVLLGLAVAVLALAVG+LYVLSSSKKSKSRGCLDPE+FKEFKLIKRVQLSH
Sbjct: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEVFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. NCBI nr
Match: XP_008455773.1 (PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo] >KAA0043648.1 NADH--cytochrome b5 reductase 1 [Cucumis melo var. makuwa] >TYK09714.1 NADH--cytochrome b5 reductase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 559.3 bits (1440), Expect = 1.9e-155
Identity = 272/281 (96.80%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60
           MDFLQSLDPQ+LLGLAVAVLALAVG+LYVLSSSKKSKSRGCLDPEIFKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

[Close]

BLAST of CmoCh03G002030 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 468.4 bits (1204), Expect = 4.1e-132
Identity = 226/280 (80.71%), Postives = 250/280 (89.29%), Query Frame = 0


Query: 2   DFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHN 61
           +FL++LD Q+LLG+ VA +A+  G+ Y L+SSK  K R CLDPE FKEFKL+KR QLSHN
Sbjct: 4   EFLRTLDRQILLGVFVAFVAVGAGAAYFLTSSK--KRRVCLDPENFKEFKLVKRHQLSHN 63

Query: 62  VAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQ 121
           VAKF F LP  TSVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQ
Sbjct: 64  VAKFVFELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQ 123

Query: 122 GRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPND 181
           GRMSHHFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D
Sbjct: 124 GRMSHHFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTD 183

Query: 182 ETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQT 241
           +TKVHLIYANVTY+DILLKEEL+ L  +YP+ FK++YVLNQPPE WDGGVGFVSKEMIQT
Sbjct: 184 KTKVHLIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQT 243

Query: 242 HCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 282
           HCPAPASDIQ+LRCGPPPMNKAM A+LEALGY+PEM F F
Sbjct: 244 HCPAPASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

[Close]

BLAST of CmoCh03G002030 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 214.9 bits (546), Expect = 8.1e-56
Identity = 109/273 (39.93%), Postives = 168/273 (61.54%), Query Frame = 0


Query: 27  LYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISC 86
           +Y+  + +++  +  L+P+ + EFKL    ++SHN   F F+  +P++ LGL +   +  
Sbjct: 58  VYLDQAKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSF-DPSAELGLHVASCLLT 117

Query: 87  R-----GKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKG 146
           R       +G+ + VI+PYTPI+     GYF+L+IK+YP G+MS HF  ++ GD L VKG
Sbjct: 118 RAPLGYNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKG 177

Query: 147 PKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKE 206
           P  +F+Y P   +  GM+AGGSGITPM QV  AI++NP D T++ L+YANV+ +DILLK+
Sbjct: 178 PVEKFKYSPNMKKHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQ 237

Query: 207 ELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQVLRCGPPPMN 266
           +LD+L  ++P + K++Y ++ P + W GGVG++SK+M     P P  D  +L CGPP M 
Sbjct: 238 KLDVLQANHP-NLKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMM 297

Query: 267 KAMGAH-------------LEALGYAPEMLFMF 282
           + +                L+ LGY  EM+F F
Sbjct: 298 EHISGGKAPDWSQGEVKGILKELGYTEEMVFKF 328

[Close]

BLAST of CmoCh03G002030 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 174.5 bits (441), Expect = 1.2e-43
Identity = 98/253 (38.74%), Postives = 145/253 (57.31%), Query Frame = 0


Query: 42  LDPEIFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYT 101
           ++P      +L+++  +SH+V KF F LP    VLGLP+G+HI         +  ++ YT
Sbjct: 657 VNPRAKVPVQLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYT 716

Query: 102 PITLDSDVGYFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY---- 161
           P +    VGYFELV+K+Y     P+    G MS +   + +G  L +KGP G   Y    
Sbjct: 717 PSSTVDVVGYFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKG 776

Query: 162 ------QPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEE 221
                 +P       MLAGG+GITP+YQ+ +AIL++P DET++++IYAN T EDILL+EE
Sbjct: 777 SFTVHGKPKFADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREE 836

Query: 222 LDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCP-APASDIQVLRCGPPPMN 274
           LD  A+ YPD  K++YV+    E W    GF+S+ +++ H P         + CGPPPM 
Sbjct: 837 LDGWAEQYPDRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMI 896

[Close]

BLAST of CmoCh03G002030 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 165.2 bits (417), Expect = 7.4e-41
Identity = 86/244 (35.25%), Postives = 141/244 (57.79%), Query Frame = 0


Query: 51  KLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVG 110
           +LI++  +SH+V KF F LP+    LGLP+G+H+         +  ++ YTP +    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 111 YFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY----------QPG 170
           + +LV+K+Y     P+    G MS H   + +G  + +KGP G   Y          +P 
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 171 QVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKSYP 230
             +   MLAGG+GITP+YQ+ ++IL +P DET+++++YAN T +DIL++EEL+  A  + 
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 231 DSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCP-APASDIQVLRCGPPPMNK-AMGAHLE 274
           +  K++YV+    E W    GF+++ +++ H P     +   L CGPPPM + A+  +LE
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

The following BLAST results are available for this feature:

BLAST of CmoCh03G002030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNT1	5.7e-131	80.71	NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=...	[more]
Q54NC1	1.1e-73	50.00	NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE...	[more]
Q4X0B5	6.0e-72	51.14	NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A...	[more]
Q1DWN4	3.0e-71	52.29	NADH-cytochrome b5 reductase 1 OS=Coccidioides immitis (strain RS) OX=246410 GN=...	[more]
Q4PGW7	3.9e-71	49.43	NADH-cytochrome b5 reductase 1 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23...	[more]

BLAST of CmoCh03G002030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GFP5	8.0e-160	100.00	NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114537...	[more]
A0A5A7TLD5	9.1e-156	96.80	NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3C2D1	9.1e-156	96.80	NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=...	[more]
A0A6J1HVM7	1.6e-155	97.15	NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252...	[more]
A0A0A0L226	2.9e-154	95.73	FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_...	[more]

BLAST of CmoCh03G002030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950742.1	1.6e-159	100.00	NADH--cytochrome b5 reductase 1-like [Cucurbita moschata]	[more]
KAG7033625.1	6.3e-159	99.64	NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6603441.1	1.8e-158	99.29	NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023543481.1	4.5e-157	98.22	NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_008455773.1	1.9e-155	96.80	PREDICTED: NADH--cytochrome b5 reductase 1 [Cucumis melo] >KAA0043648.1 NADH--cy...	[more]

BLAST of CmoCh03G002030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17770.1	4.1e-132	80.71	NADH:cytochrome B5 reductase 1	[more]
AT5G20080.1	8.1e-56	39.93	FAD/NAD(P)-binding oxidoreductase	[more]
AT1G37130.1	1.2e-43	38.74	nitrate reductase 2	[more]
AT1G77760.1	7.4e-41	35.25	nitrate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001834	NADH:cytochrome b5 reductase-like	PRINTS	PR00406	CYTB5RDTASE	coord: 157..176 score: 66.67 coord: 134..148 score: 49.31 coord: 251..259 score: 62.96 coord: 76..87 score: 57.47 coord: 98..105 score: 41.41 coord: 195..206 score: 64.06
IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain	PFAM	PF00970	FAD_binding_6	coord: 50..148 e-value: 7.9E-32 score: 109.6
IPR001433	Oxidoreductase FAD/NAD(P)-binding	PFAM	PF00175	NAD_binding_1	coord: 158..263 e-value: 1.1E-30 score: 106.5
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	GENE3D	3.40.50.80		coord: 140..281 e-value: 1.3E-44 score: 153.5
IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain	SUPERFAMILY	52343	Ferredoxin reductase-like, C-terminal NADP-linked domain	coord: 137..280
None	No IPR available	GENE3D	2.40.30.10	Translation factors	coord: 40..139 e-value: 4.4E-34 score: 119.1
None	No IPR available	PANTHER	PTHR19370:SF203	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..281
None	No IPR available	PANTHER	PTHR19370	NADH-CYTOCHROME B5 REDUCTASE	coord: 2..281
None	No IPR available	CDD	cd06183	cyt_b5_reduct_like	coord: 50..281 e-value: 6.26919E-118 score: 336.076
IPR017927	FAD-binding domain, ferredoxin reductase-type	PROSITE	PS51384	FAD_FR	coord: 45..149 score: 14.225162
IPR017938	Riboflavin synthase-like beta-barrel	SUPERFAMILY	63380	Riboflavin synthase domain-like	coord: 42..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G002030.1	CmoCh03G002030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004128	cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function	GO:0009703	nitrate reductase (NADH) activity
molecular_function	GO:0016491	oxidoreductase activity