CmoCh03G001160 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G001160
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Beta-galactosidase
Location	Cmo_Chr03: 1601773 .. 1602383 (+)
RNA-Seq Expression	CmoCh03G001160
Synteny	CmoCh03G001160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTACTGAAAGTTGGATGGGATGGTTCAAGAAATGGGTTGACAAAGACCCTTACAGAACCCTAGAAGATGTAGTCTTTTATGTCGCAAGATTTTTTGAATCAGGTGGTGTGTTTAACTTTCATAAATAGAAGGTACATTAGGTTGCAATGGCTCGAGTTTTGTGTTTGAGAAACCTGTTCTATTAAAATTGGGAACCAACACCATAACCCTTTTGAGCGGCACAATGAGACTGAAGAATTACGATGCATTTTATGACACGGTGCCAACATGAATTGATGGAGGTCCCATTTATGTAATTGGAGATGGAAACGTCATAACAAATTTGTCATCAAACTTGTAGTCTTATAAGGTCGGATTAAATGGAGAAATGAAGCAATTTTACAACTCGATGTTCTCACATAGAACCAAATGGAGCGCATTAAATCAAAAATCTATTGGAAGGCGAATGATGCGGTACAAAGCTAGCTTTAAGACCCCTTCGGGAACTGACCCAGTAGCATTGGACATGCAAGGAATGGGCAAAGGTCAGACTTGGGTAAAGGAGCAAATCATAGGCCGATTATGGCCTTCTTTCATTACTGGAAACGATGGTTGCAGTTCGACCTGA

mRNA sequence

ATGTTTACTGAAAGTTGGATGGGATGGTTCAAGAAATGGGTTGACAAAGACCCTTACAGAACCCTAGAAGATGTAGTCTTTTATGTCGCAAGATTTTTTGAATCAGGTGGTACATTAGGTTGCAATGGCTCGAGTTTTGTGTTTGAGAAACCTGTTCTATTAAAATTGGGAACCAACACCATAACCCTTTTGAGCGGCACAATGAGACTGAAGAATTACGATGCATTTTATGACACGTCTTATAAGGTCGGATTAAATGGAGAAATGAAGCAATTTTACAACTCGATGTTCTCACATAGAACCAAATGGAGCGCATTAAATCAAAAATCTATTGGAAGGCGAATGATGCGGTACAAAGCTAGCTTTAAGACCCCTTCGGGAACTGACCCAGTAGCATTGGACATGCAAGGAATGGGCAAAGGTCAGACTTGGGTAAAGGAGCAAATCATAGGCCGATTATGGCCTTCTTTCATTACTGGAAACGATGGTTGCAGTTCGACCTGA

Coding sequence (CDS)

ATGTTTACTGAAAGTTGGATGGGATGGTTCAAGAAATGGGTTGACAAAGACCCTTACAGAACCCTAGAAGATGTAGTCTTTTATGTCGCAAGATTTTTTGAATCAGGTGGTACATTAGGTTGCAATGGCTCGAGTTTTGTGTTTGAGAAACCTGTTCTATTAAAATTGGGAACCAACACCATAACCCTTTTGAGCGGCACAATGAGACTGAAGAATTACGATGCATTTTATGACACGTCTTATAAGGTCGGATTAAATGGAGAAATGAAGCAATTTTACAACTCGATGTTCTCACATAGAACCAAATGGAGCGCATTAAATCAAAAATCTATTGGAAGGCGAATGATGCGGTACAAAGCTAGCTTTAAGACCCCTTCGGGAACTGACCCAGTAGCATTGGACATGCAAGGAATGGGCAAAGGTCAGACTTGGGTAAAGGAGCAAATCATAGGCCGATTATGGCCTTCTTTCATTACTGGAAACGATGGTTGCAGTTCGACCTGA

Protein sequence

MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNTITLLSGTMRLKNYDAFYDTSYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCSST

Homology

[Close]

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot
Match: P49676 (Beta-galactosidase OS=Brassica oleracea OX=3712 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.3e-18
Identity = 54/155 (34.84%), Postives = 78/155 (50.32%), Query Frame = 0


Query: 42  NGSSFVFEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------------- 101
           N   + FEK V L  GTN + LLS ++ L+NY  F+++                      
Sbjct: 521 NKFDYRFEKKVNLVHGTNHLALLSVSVGLQNYGPFFESGPTGINGPVKLVGYKGDETIEK 580

Query: 102 -------SYKVGLNGEMKQFYN--SMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDP 161
                   YK+GLNG   + ++  S   H  KWS   +    R +  YKA+FK P G DP
Sbjct: 581 DLSKHQWDYKIGLNGFNHKLFSMKSAGHHHRKWST-EKLPADRMLSWYKANFKAPLGKDP 640

Query: 162 VALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCS 166
           V +D+ G+GKG+ W+  Q IGR WPSF + ++GC+
Sbjct: 641 VIVDLNGLGKGEVWINGQSIGRYWPSFNSSDEGCT 674

HSP 2 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 32/74 (43.24%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWFK W  K PYRT ED+ F VARFF++GGT         F+   +   GTN 
Sbjct: 249 MWTENWTGWFKNWGGKHPYRTAEDLAFSVARFFQTGGT---------FQNYYMYHGGTNF 308

Query: 61  ITLLSGTMRLKNYD 75
             +  G     +YD
Sbjct: 309 GRVAGGPYITTSYD 313

[Close]

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot
Match: Q0INM3 (Beta-galactosidase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0429200 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.7e-18
Identity = 59/171 (34.50%), Postives = 80/171 (46.78%), Query Frame = 0


Query: 24  DVVFYVARFFESGGTLGCNGSSFV-FEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT--- 83
           D +  VAR F +G   G     +V  ++P+ L  G N +TLLS  + L+NY AF +    
Sbjct: 567 DKIRDVARVFVNGKLAGSQVGHWVSLKQPIQLVEGLNELTLLSEIVGLQNYGAFLEKDGA 626

Query: 84  -----------------------SYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMM 143
                                  +Y+VGL GE    Y         WS + + S+ +   
Sbjct: 627 GFRGQVTLTGLSDGDVDLTNSLWTYQVGLKGEFSMIYAPEKQGCAGWSRMQKDSV-QPFT 686

Query: 144 RYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCSST 168
            YK  F TP GTDPVA+D+  MGKGQ WV   +IGR W S +    GCSS+
Sbjct: 687 WYKTMFSTPKGTDPVAIDLGSMGKGQAWVNGHLIGRYW-SLVAPESGCSSS 735

[Close]

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot
Match: Q10NX8 (Beta-galactosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0255100 PE=1 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 54/156 (34.62%), Postives = 72/156 (46.15%), Query Frame = 0


Query: 35  SGGTLGCNGSSFV-FEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT-------------- 94
           +G   G   SS +  + PV L  G N I LLS T+ L NY AF+D               
Sbjct: 531 AGSAKGSASSSLISLQTPVTLVPGKNKIDLLSTTVGLSNYGAFFDLVGAGVTGPVKLSGP 590

Query: 95  -----------SYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGT 154
                      +Y++GL GE    YN       +W + N     + ++ YK  F  P+G 
Sbjct: 591 NGALNLSSTDWTYQIGLRGEDLHLYNPS-EASPEWVSDNAYPTNQPLIWYKTKFTAPAGD 650

Query: 155 DPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGC 165
           DPVA+D  GMGKG+ WV  Q IGR WP+ +    GC
Sbjct: 651 DPVAIDFTGMGKGEAWVNGQSIGRYWPTNLAPQSGC 685

HSP 2 Score: 50.1 bits (118), Expect = 2.9e-05
Identity = 27/74 (36.49%), Postives = 36/74 (48.65%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWF  +    PYR  ED+ F VARF++ GGT         F+   +   GTN 
Sbjct: 255 MWTENWSGWFLSFGGAVPYRPAEDLAFAVARFYQRGGT---------FQNYYMYHGGTNF 314

Query: 61  ITLLSGTMRLKNYD 75
                G     +YD
Sbjct: 315 GRSTGGPFIATSYD 319

[Close]

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot
Match: Q9SCV5 (Beta-galactosidase 7 OS=Arabidopsis thaliana OX=3702 GN=BGAL7 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 9.6e-17
Identity = 49/138 (35.51%), Postives = 71/138 (51.45%), Query Frame = 0


Query: 57  GTNTITLLSGTMRLKNYDAFYDT-----------------------------SYKVGLNG 116
           GTN I+LLS ++ L+NY  F+++                              YK+GLNG
Sbjct: 536 GTNHISLLSVSVGLQNYGPFFESGPTGINGPVSLVGYKGEETIEKDLSQHQWDYKIGLNG 595

Query: 117 EMKQFYNSMFSHRTKWSALNQK-SIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVK 165
              + ++       KW+  N+K   GR +  YKA FK P G +PV +D+ G+GKG+ W+ 
Sbjct: 596 YNDKLFSIKSVGHQKWA--NEKLPTGRMLTWYKAKFKAPLGKEPVIVDLNGLGKGEAWIN 655

HSP 2 Score: 64.3 bits (155), Expect = 1.5e-09
Identity = 38/102 (37.25%), Postives = 50/102 (49.02%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWFK W  K PYRT ED+ F VARFF++GGT         F+   +   GTN 
Sbjct: 250 MWTENWTGWFKNWGGKHPYRTAEDLAFSVARFFQTGGT---------FQNYYMYHGGTNF 309

Query: 61  ITLLSGTMRLKNYDAFYDTSYKVGLN----GEMKQFYNSMFS 99
             +  G     +YD          LN    G +KQ +  + S
Sbjct: 310 GRVAGGPYITTSYDYHAPLDEFGNLNQPKWGHLKQLHTVLKS 342

[Close]

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot
Match: Q9SCV4 (Beta-galactosidase 8 OS=Arabidopsis thaliana OX=3702 GN=BGAL8 PE=2 SV=2)

HSP 1 Score: 85.9 bits (211), Expect = 4.8e-16
Identity = 51/144 (35.42%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 51  PVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------------------SYKV 110
           P+ L  GTNTI LLS T+ L NY AF+D                            +Y+V
Sbjct: 539 PINLVTGTNTIDLLSVTVGLANYGAFFDLVGAGITGPVTLKSAKGGSSIDLASQQWTYQV 598

Query: 111 GLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQT 168
           GL GE            ++W + +     + ++ YK +F  PSG++PVA+D  G GKG  
Sbjct: 599 GLKGEDTGLAT---VDSSEWVSKSPLPTKQPLIWYKTTFDAPSGSEPVAIDFTGTGKGIA 658

HSP 2 Score: 54.7 bits (130), Expect = 1.2e-06
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWF  + D  PYR +ED+ F VARF++ GGT         F+   +   GTN 
Sbjct: 254 MWTENWSGWFLGFGDPSPYRPVEDLAFAVARFYQRGGT---------FQNYYMYHGGTNF 313

Query: 61  ITLLSGTMRLKNYD 75
                G +   +YD
Sbjct: 314 DRTSGGPLISTSYD 318

[Close]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL
Match: A0A5D3DUP4 (Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G00870 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 6.4e-48
Identity = 110/196 (56.12%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 473 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 532

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 533 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 592

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP+G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 593 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPAGIDPVVLDMQGMGKGQAWVNGQSIG 652

[Close]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL
Match: A0A5A7V9Y9 (Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold130G00750 PE=3 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 6.4e-48
Identity = 110/196 (56.12%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 473 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 532

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 533 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 592

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP+G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 593 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPAGIDPVVLDMQGMGKGQAWVNGQSIG 652

[Close]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL
Match: A0A1S3CP60 (beta-galactosidase 7-like OS=Cucumis melo OX=3656 GN=LOC103503198 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 8.4e-48
Identity = 110/196 (56.12%), Postives = 119/196 (60.71%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 139 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 198

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 199 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 258

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 259 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPGGIDPVVLDMQGMGKGQAWVNGQSIG 318

[Close]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL
Match: A0A0A0LDH9 (Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.4e-47
Identity = 101/159 (63.52%), Postives = 108/159 (67.92%), Query Frame = 0


Query: 36  GGTLGCNGSSFVFEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------- 95
           G   G NG SFVFEKP+ LKLGTNTITLLS T+ LKNYDAFYDT                
Sbjct: 497 GSQWGSNGQSFVFEKPIQLKLGTNTITLLSATVGLKNYDAFYDTVPTGIDGGPIYLIGDG 556

Query: 96  -----------SYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGT 155
                      SYKVGLNGE KQ YN MFS+RTKWS LN+KSIGRRM  +KA+FKTPSGT
Sbjct: 557 NVTTDLSSNLWSYKVGLNGERKQLYNPMFSNRTKWSTLNKKSIGRRMTWFKATFKTPSGT 616

Query: 156 DPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCSST 168
           DPV LDMQGMGKGQ WV  + IGR WPSFI  ND CS T
Sbjct: 617 DPVVLDMQGMGKGQAWVNGRSIGRFWPSFIASNDSCSET 655

[Close]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL
Match: A0A0A0LDH9 (Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.5e-09
Identity = 30/39 (76.92%), Postives = 34/39 (87.18%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTL 40
           MFTE+W+GWFKKW DKDP+RT EDV F VARFF+SGG L
Sbjct: 249 MFTENWVGWFKKWGDKDPHRTAEDVAFSVARFFQSGGIL 287

HSP 2 Score: 198.0 bits (502), Expect = 3.2e-47
Identity = 109/198 (55.05%), Postives = 120/198 (60.61%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDV------------VFYVARFFESGGTLGCNGSSFVFEKPVLLKL 68
           W+   VD +   +L++V            VF   R+   G   G NG SFVFEKP+LLK 
Sbjct: 438 WYMTNVDTNGTSSLQNVTLQVNTKGHVLHVFVNKRYI--GSQWGSNGKSFVFEKPILLKS 497

Query: 69  GTNTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEM 128
            TNTITLLS T+ LKNYDAFYD                            SYKVGLNGEM
Sbjct: 498 RTNTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVTTDLSSNLWSYKVGLNGEM 557

Query: 129 KQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQI 168
           KQ YN MFS RT W ALNQKSIGRRM  YK SFKTP+G DPV LDMQGMGKGQ WV E+ 
Sbjct: 558 KQIYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPAGIDPVVLDMQGMGKGQAWVNEKS 617

[Close]

BLAST of CmoCh03G001160 vs. NCBI nr
Match: XP_038896535.1 (beta-galactosidase 15-like [Benincasa hispida])

HSP 1 Score: 201.4 bits (511), Expect = 5.9e-48
Identity = 102/159 (64.15%), Postives = 108/159 (67.92%), Query Frame = 0


Query: 36  GGTLGCNGSSFVFEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------- 95
           G   G NG SFVFEKP+LLK+GTNTITLLS T+ LKNYDAFYDT                
Sbjct: 513 GSQWGSNGQSFVFEKPILLKVGTNTITLLSATVGLKNYDAFYDTVPTGIDGGPIYLIGDG 572

Query: 96  -----------SYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGT 155
                      SYKVGLNGE+KQ YN MFS RTKWS LN+KSIGRRM  YK SFKTPSG 
Sbjct: 573 NVTTDLSSNLWSYKVGLNGEIKQLYNPMFSQRTKWSTLNKKSIGRRMTWYKTSFKTPSGI 632

Query: 156 DPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCSST 168
           DPV  DMQGMGKGQ WV  Q IGR WPSFI GND CS+T
Sbjct: 633 DPVTFDMQGMGKGQAWVNGQSIGRFWPSFIAGNDSCSAT 671

[Close]

BLAST of CmoCh03G001160 vs. NCBI nr
Match: KAA0062481.1 (beta-galactosidase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 200.3 bits (508), Expect = 1.3e-47
Identity = 110/196 (56.12%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 473 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 532

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 533 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 592

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP+G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 593 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPAGIDPVVLDMQGMGKGQAWVNGQSIG 652

[Close]

BLAST of CmoCh03G001160 vs. NCBI nr
Match: TYK27403.1 (beta-galactosidase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 200.3 bits (508), Expect = 1.3e-47
Identity = 110/196 (56.12%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 473 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 532

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 533 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 592

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP+G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 593 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPAGIDPVVLDMQGMGKGQAWVNGQSIG 652

[Close]

BLAST of CmoCh03G001160 vs. NCBI nr
Match: XP_008465565.1 (PREDICTED: beta-galactosidase 7-like, partial [Cucumis melo])

HSP 1 Score: 199.9 bits (507), Expect = 1.7e-47
Identity = 110/196 (56.12%), Postives = 119/196 (60.71%), Query Frame = 0


Query: 9   WFKKWVDKDPYRTLEDVVF------YVARFFES----GGTLGCNGSSFVFEKPVLLKLGT 68
           W+   VD +   +L++V        +V   F +    G   G NG SFVFEKPVLLK GT
Sbjct: 139 WYMTSVDTNGTSSLQNVTLQVNTKGHVLHAFVNKRYIGSQWGSNGQSFVFEKPVLLKSGT 198

Query: 69  NTITLLSGTMRLKNYDAFYDT---------------------------SYKVGLNGEMKQ 128
           NTITLLS T+ LKNYDAFYD                            SYKVGLNGEMKQ
Sbjct: 199 NTITLLSATVGLKNYDAFYDMVPTGIDGGPIYLIGDGNVKTDLSSNLWSYKVGLNGEMKQ 258

Query: 129 FYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVKEQIIG 168
            YN MFS RT W ALNQKSIGRRM  YK SFKTP G DPV LDMQGMGKGQ WV  Q IG
Sbjct: 259 IYNPMFSQRTNWIALNQKSIGRRMTWYKTSFKTPGGIDPVVLDMQGMGKGQAWVNGQSIG 318

[Close]

BLAST of CmoCh03G001160 vs. NCBI nr
Match: KAE8650760.1 (hypothetical protein Csa_023866, partial [Cucumis sativus])

HSP 1 Score: 198.4 bits (503), Expect = 5.0e-47
Identity = 101/159 (63.52%), Postives = 108/159 (67.92%), Query Frame = 0


Query: 36  GGTLGCNGSSFVFEKPVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------- 95
           G   G NG SFVFEKP+ LKLGTNTITLLS T+ LKNYDAFYDT                
Sbjct: 171 GSQWGSNGQSFVFEKPIQLKLGTNTITLLSATVGLKNYDAFYDTVPTGIDGGPIYLIGDG 230

Query: 96  -----------SYKVGLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGT 155
                      SYKVGLNGE KQ YN MFS+RTKWS LN+KSIGRRM  +KA+FKTPSGT
Sbjct: 231 NVTTDLSSNLWSYKVGLNGERKQLYNPMFSNRTKWSTLNKKSIGRRMTWFKATFKTPSGT 290

Query: 156 DPVALDMQGMGKGQTWVKEQIIGRLWPSFITGNDGCSST 168
           DPV LDMQGMGKGQ WV  + IGR WPSFI  ND CS T
Sbjct: 291 DPVVLDMQGMGKGQAWVNGRSIGRFWPSFIASNDSCSET 329

[Close]

BLAST of CmoCh03G001160 vs. TAIR 10
Match: AT5G20710.1 (beta-galactosidase 7 )

HSP 1 Score: 88.2 bits (217), Expect = 6.8e-18
Identity = 49/138 (35.51%), Postives = 71/138 (51.45%), Query Frame = 0


Query: 57  GTNTITLLSGTMRLKNYDAFYDT-----------------------------SYKVGLNG 116
           GTN I+LLS ++ L+NY  F+++                              YK+GLNG
Sbjct: 536 GTNHISLLSVSVGLQNYGPFFESGPTGINGPVSLVGYKGEETIEKDLSQHQWDYKIGLNG 595

Query: 117 EMKQFYNSMFSHRTKWSALNQK-SIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQTWVK 165
              + ++       KW+  N+K   GR +  YKA FK P G +PV +D+ G+GKG+ W+ 
Sbjct: 596 YNDKLFSIKSVGHQKWA--NEKLPTGRMLTWYKAKFKAPLGKEPVIVDLNGLGKGEAWIN 655

HSP 2 Score: 64.3 bits (155), Expect = 1.1e-10
Identity = 38/102 (37.25%), Postives = 50/102 (49.02%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWFK W  K PYRT ED+ F VARFF++GGT         F+   +   GTN 
Sbjct: 250 MWTENWTGWFKNWGGKHPYRTAEDLAFSVARFFQTGGT---------FQNYYMYHGGTNF 309

Query: 61  ITLLSGTMRLKNYDAFYDTSYKVGLN----GEMKQFYNSMFS 99
             +  G     +YD          LN    G +KQ +  + S
Sbjct: 310 GRVAGGPYITTSYDYHAPLDEFGNLNQPKWGHLKQLHTVLKS 342

[Close]

BLAST of CmoCh03G001160 vs. TAIR 10
Match: AT2G28470.1 (beta-galactosidase 8 )

HSP 1 Score: 85.9 bits (211), Expect = 3.4e-17
Identity = 51/144 (35.42%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 51  PVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------------------SYKV 110
           P+ L  GTNTI LLS T+ L NY AF+D                            +Y+V
Sbjct: 539 PINLVTGTNTIDLLSVTVGLANYGAFFDLVGAGITGPVTLKSAKGGSSIDLASQQWTYQV 598

Query: 111 GLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQT 168
           GL GE            ++W + +     + ++ YK +F  PSG++PVA+D  G GKG  
Sbjct: 599 GLKGEDTGLAT---VDSSEWVSKSPLPTKQPLIWYKTTFDAPSGSEPVAIDFTGTGKGIA 658

HSP 2 Score: 54.7 bits (130), Expect = 8.4e-08
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWF  + D  PYR +ED+ F VARF++ GGT         F+   +   GTN 
Sbjct: 254 MWTENWSGWFLGFGDPSPYRPVEDLAFAVARFYQRGGT---------FQNYYMYHGGTNF 313

Query: 61  ITLLSGTMRLKNYD 75
                G +   +YD
Sbjct: 314 DRTSGGPLISTSYD 318

[Close]

BLAST of CmoCh03G001160 vs. TAIR 10
Match: AT2G28470.2 (beta-galactosidase 8 )

HSP 1 Score: 85.9 bits (211), Expect = 3.4e-17
Identity = 51/144 (35.42%), Postives = 70/144 (48.61%), Query Frame = 0


Query: 51  PVLLKLGTNTITLLSGTMRLKNYDAFYDT---------------------------SYKV 110
           P+ L  GTNTI LLS T+ L NY AF+D                            +Y+V
Sbjct: 533 PINLVTGTNTIDLLSVTVGLANYGAFFDLVGAGITGPVTLKSAKGGSSIDLASQQWTYQV 592

Query: 111 GLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQT 168
           GL GE            ++W + +     + ++ YK +F  PSG++PVA+D  G GKG  
Sbjct: 593 GLKGEDTGLAT---VDSSEWVSKSPLPTKQPLIWYKTTFDAPSGSEPVAIDFTGTGKGIA 652

HSP 2 Score: 54.7 bits (130), Expect = 8.4e-08
Identity = 28/74 (37.84%), Postives = 39/74 (52.70%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           M+TE+W GWF  + D  PYR +ED+ F VARF++ GGT         F+   +   GTN 
Sbjct: 248 MWTENWSGWFLGFGDPSPYRPVEDLAFAVARFYQRGGT---------FQNYYMYHGGTNF 307

Query: 61  ITLLSGTMRLKNYD 75
                G +   +YD
Sbjct: 308 DRTSGGPLISTSYD 312

[Close]

BLAST of CmoCh03G001160 vs. TAIR 10
Match: AT2G32810.1 (beta galactosidase 9 )

HSP 1 Score: 85.1 bits (209), Expect = 5.8e-17
Identity = 49/144 (34.03%), Postives = 69/144 (47.92%), Query Frame = 0


Query: 50  KPVLLKLGTNTITLLSGTMRLKNYDAFYDT--------------------------SYKV 109
           +PV    G N + LL+ T+ L+NY AF +                           +Y+V
Sbjct: 560 QPVRFIQGNNDLLLLTQTVGLQNYGAFLEKDGAGFRGKAKLTGFKNGDLDLSKSSWTYQV 619

Query: 110 GLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQT 168
           GL GE  + Y    + + +WS L   +     M YK  F  P+GTDPV L+++ MG+GQ 
Sbjct: 620 GLKGEADKIYTVEHNEKAEWSTLETDASPSIFMWYKTYFDPPAGTDPVVLNLESMGRGQA 679

HSP 2 Score: 51.6 bits (122), Expect = 7.1e-07
Identity = 31/91 (34.07%), Postives = 44/91 (48.35%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           ++TE W GW+ KW    P+R  ED+ F VARF++ GG+         F+   +   GTN 
Sbjct: 260 LWTEDWDGWYTKWGGSLPHRPAEDLAFAVARFYQRGGS---------FQNYYMYFGGTNF 319

Query: 61  ITLLSGTMRLKNYDAFYDTSY-KVGLNGEMK 91
                G   + +YD  YD    + GL  E K
Sbjct: 320 GRTSGGPFYITSYD--YDAPLDEYGLRSEPK 339

[Close]

BLAST of CmoCh03G001160 vs. TAIR 10
Match: AT2G32810.2 (beta galactosidase 9 )

HSP 1 Score: 85.1 bits (209), Expect = 5.8e-17
Identity = 49/144 (34.03%), Postives = 69/144 (47.92%), Query Frame = 0


Query: 50  KPVLLKLGTNTITLLSGTMRLKNYDAFYDT--------------------------SYKV 109
           +PV    G N + LL+ T+ L+NY AF +                           +Y+V
Sbjct: 560 QPVRFIQGNNDLLLLTQTVGLQNYGAFLEKDGAGFRGKAKLTGFKNGDLDLSKSSWTYQV 619

Query: 110 GLNGEMKQFYNSMFSHRTKWSALNQKSIGRRMMRYKASFKTPSGTDPVALDMQGMGKGQT 168
           GL GE  + Y    + + +WS L   +     M YK  F  P+GTDPV L+++ MG+GQ 
Sbjct: 620 GLKGEADKIYTVEHNEKAEWSTLETDASPSIFMWYKTYFDPPAGTDPVVLNLESMGRGQA 679

HSP 2 Score: 51.6 bits (122), Expect = 7.1e-07
Identity = 31/91 (34.07%), Postives = 44/91 (48.35%), Query Frame = 0


Query: 1   MFTESWMGWFKKWVDKDPYRTLEDVVFYVARFFESGGTLGCNGSSFVFEKPVLLKLGTNT 60
           ++TE W GW+ KW    P+R  ED+ F VARF++ GG+         F+   +   GTN 
Sbjct: 260 LWTEDWDGWYTKWGGSLPHRPAEDLAFAVARFYQRGGS---------FQNYYMYFGGTNF 319

Query: 61  ITLLSGTMRLKNYDAFYDTSY-KVGLNGEMK 91
                G   + +YD  YD    + GL  E K
Sbjct: 320 GRTSGGPFYITSYD--YDAPLDEYGLRSEPK 339

The following BLAST results are available for this feature:

BLAST of CmoCh03G001160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49676	2.3e-18	34.84	Beta-galactosidase OS=Brassica oleracea OX=3712 PE=2 SV=1	[more]
Q0INM3	8.7e-18	34.50	Beta-galactosidase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0429200 P...	[more]
Q10NX8	1.5e-17	34.62	Beta-galactosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0255100 PE...	[more]
Q9SCV5	9.6e-17	35.51	Beta-galactosidase 7 OS=Arabidopsis thaliana OX=3702 GN=BGAL7 PE=2 SV=2	[more]
Q9SCV4	4.8e-16	35.42	Beta-galactosidase 8 OS=Arabidopsis thaliana OX=3702 GN=BGAL8 PE=2 SV=2	[more]

BLAST of CmoCh03G001160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DUP4	6.4e-48	56.12	Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold325G0...	[more]
A0A5A7V9Y9	6.4e-48	56.12	Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold130G0...	[more]
A0A1S3CP60	8.4e-48	56.12	beta-galactosidase 7-like OS=Cucumis melo OX=3656 GN=LOC103503198 PE=4 SV=1	[more]
A0A0A0LDH9	2.4e-47	63.52	Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1	[more]
A0A0A0LDH9	1.5e-09	76.92	Beta-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_3G550690 PE=3 SV=1	[more]

BLAST of CmoCh03G001160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896535.1	5.9e-48	64.15	beta-galactosidase 15-like [Benincasa hispida]	[more]
KAA0062481.1	1.3e-47	56.12	beta-galactosidase 7-like [Cucumis melo var. makuwa]	[more]
TYK27403.1	1.3e-47	56.12	beta-galactosidase 7-like [Cucumis melo var. makuwa]	[more]
XP_008465565.1	1.7e-47	56.12	PREDICTED: beta-galactosidase 7-like, partial [Cucumis melo]	[more]
KAE8650760.1	5.0e-47	63.52	hypothetical protein Csa_023866, partial [Cucumis sativus]	[more]

BLAST of CmoCh03G001160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20710.1	6.8e-18	35.51	beta-galactosidase 7	[more]
AT2G28470.1	3.4e-17	35.42	beta-galactosidase 8	[more]
AT2G28470.2	3.4e-17	35.42	beta-galactosidase 8	[more]
AT2G32810.1	5.8e-17	34.03	beta galactosidase 9	[more]
AT2G32810.2	5.8e-17	34.03	beta galactosidase 9	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001944	Glycoside hydrolase, family 35	PRINTS	PR00742	GLHYDRLASE35	coord: 1..13 score: 35.66 coord: 113..127 score: 34.12 coord: 140..156 score: 49.13
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 42..79
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 1..39
IPR001944	Glycoside hydrolase, family 35	PANTHER	PTHR23421	BETA-GALACTOSIDASE RELATED	coord: 80..165
None	No IPR available	GENE3D	2.60.120.260		coord: 77..162 e-value: 2.8E-7 score: 32.7
None	No IPR available	PANTHER	PTHR23421:SF88	BETA-GALACTOSIDASE 14	coord: 80..165
None	No IPR available	PANTHER	PTHR23421:SF88	BETA-GALACTOSIDASE 14	coord: 1..39
None	No IPR available	PANTHER	PTHR23421:SF88	BETA-GALACTOSIDASE 14	coord: 42..79
IPR008979	Galactose-binding-like domain superfamily	SUPERFAMILY	49785	Galactose-binding domain-like	coord: 80..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G001160.1	CmoCh03G001160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds