CmoCh03G001000 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G001000
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionAdenylosuccinate synthetase, chloroplastic
LocationCmo_Chr03: 1429117 .. 1432346 (+)
RNA-Seq ExpressionCmoCh03G001000
SyntenyCmoCh03G001000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATCTCTTCTCTTGCTTTCCAGCCCACTCTTTTCTCCGCCGCCAAATTGTCGTCGCCGGCCTGCCGGAGACCCTTTTCCGGACGTCATCCTGCACTCGTCGTTTGCTCCTCTGCTAAACCCGCGGCGGCGGTTAGTTCGTCTGTCAGTGCGCCGGAGTCAGCGGGGAAGGATGAGCTGAAACGAATTGGGGAACTTAGTCAAGTATCGGGAGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGATATCTTGGCACAGCACTTCGAAATCGTCGCTCGTTGTCAGGTAGTCTTCAAGATTTGATTTCATTTTTCTTTTGGGATAATTTTTCTGTTCAGGTTTTTGTTGTGTTTCTTCTGCTTTTTCTATTGGATTTGGATTTGTTTATTGATTTGCTCTATTTCTACTTGCCTCCATGCGAACCTCGTTTTATTTTGTTTGCAATTTCAGAATCTCTTTGTTCTTTTTGTTCTTTCTTTCCAGAGGCACTTTGACGTTATCGAAGCATATAAGAATCTTCTTCTAACCTTGTGTTGTTATATTTGCAATGGTGAAGGCCAAAGGGTGGCTATGATTTATCTCTGTATTTGCACATCCGGTTTTAGAAGAGTCGATGAGCCATTTTGATTCTATGTTGCGTTTTTATGTGATATCCTGATGTGTAGTATGACACCTTTGGTTTCTGGAACTGTCTGAGAGAGTTCAAACTGATTTGCAGGGTGGGGCTAATGCTGGGCACACAATCTATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATCCCTTCAGGGATCCTTAATGAAGGTACTTTATGTGTTGTTGGAAACGGAGTTGTGGTGCACGTGCCCGGTTTCTTCGAAGAAATCGACCGTCTAGAAGCGAGTGGAGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTTCATCAGGAGGTGGATGGCTTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGCAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGATACACTTCCTCAGAAGCTCGACCTTCTACTCTCCGATGCTGCGTCGAGATTCAAGGATTTTGTCTATACCCCAGAAATTCTGAAGGAAGAAGTCGAGAAGTACAAACGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGCATTTCATCAATGAAGCCATCTCTGATAATAAGAAAATTTTGGTTGAAGGTGGTCAAGCAACTATGCTTGACATCGACTTTGGTACTTATCCTTTCGTAACTTCTTCCAGCCCGTCCGCAGGTGGAATTTGCACCGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAGTTTCTTTTGATTCACTGTTTCTTTATTTCCTTCTTGATGGAAAGCTGATTTATATATTGGATGTGGTGCATATCCTTAAGGTTCTCTGTTGATCCTTGCAGGTGAAGGCATACACTACTAGAGTTGGTTCTGGCCCTTTTCCTACTGAGATCTTGGGAAAGGGGGGTGACCTCCTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGCCCTCGTCGCTGTGGATGGCTCGATATAGTTGCTCTAAAATATTGCTGTAAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTCGATGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTTTCTTATCGAGCACCTGATGGAACACCAGTCGAATCATTTCCCGCAGATCTTAGTCTTCTTGAACAAATAAAGGCAAGCATTATGCATCCTTTTTCCTTGCTTTTATTTTAACTTCGATGCTATGGGTTTAGTTAATGGTTTTAGAATTGTGACTTTTAGGTGTTCTTAAGAAGAAGAAATAAAAGAAAGTAAGAGAATAAAAGATAAATAAAAGGCTGGAGAAGACCTTTCACACGTGTTCTTTCATTGTTACTTAAGTTGAAAGTTTGGGGAGTATTGTGCTGCAAGATTTTGAATGCTCTTTGGTAGTTTATGTCTCTATAAAGAACAGTATTACTCACTTGTTTATGCTTGGTGCATATGGCTTTAGAAGACCAGCCAAAAACTTTATAGGTTGATGTCATTTATTCTTGTGTTAACTTTTCAAAAATCAGCAAAAAGTTGTGTTTCAAAAAAGATAAAAATCTGACGTGCATTTAGTTGTGTTTGAATATGCTCTCACGTGTTCTTTCGTTGTTACTTCATTAAAGTGTTTGGAATGGTTGTGTAACAGCCCAAGCTCACCGCTACACCGGGCGGTGTTCCAGTGAGGACGCTGGGTTCCGAAGGGGGTGGATTGTGAGATCCCATATCGGTTGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAATCTCTCCCTAACAGATGCGTTTTAAAAACCTTGAGGGAAGCCCGAAAGGGAAAATCCCAAAAGGAAAATATCTGCTAGCAGTGGGCTTGGATTGTTACAGTTTGGGATTATGTTCGACCAAGATTTTGGATGTTTTTTGGATGTTTTCGGGCTTTTGATTGTCTTGATAAAGAATACGATTTCTTTTATTTTTTTATTATGTTGCATGCATATCTTTGGACGTGTGGTCTTTTAGGCAAATGCATTAGAAAACCATATTATTTCGAAAAGGAAATGAAATGCTTTAAGTTTCAAGTAAAAAATTAGTCAACCGGAGTATAGTTCATAGTTAAAGACATTATAACATTGATCAAGGTCGGAAGTTTGAATCTCCACCCCTGTTATTGAACAAAAAAAAAGCGTTTTTAATATTGTCTAAAGTTCTTTGATGTTGTCCATGGTTATTAGCATTTTCTTTGTTTGCAACTGTATCATGTTATATTGCTCTTTTGTTTCCAAGTCATAAACTAAATTAGGTGTTCAAATTTTCTTAAATTTCAAGGTCTTCAACTCCTGAATCCAGAATCTTACAACAGTCTTACTTTTTCCCGTTTGTTTTCCTGCGTATAGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCAAAAGAATATGTAACAAGGATCGAAGAACTCGTGGGCATACCCGTGCATTACATCGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

mRNA sequence

ATGAGCATCTCTTCTCTTGCTTTCCAGCCCACTCTTTTCTCCGCCGCCAAATTGTCGTCGCCGGCCTGCCGGAGACCCTTTTCCGGACGTCATCCTGCACTCGTCGTTTGCTCCTCTGCTAAACCCGCGGCGGCGGTTAGTTCGTCTGTCAGTGCGCCGGAGTCAGCGGGGAAGGATGAGCTGAAACGAATTGGGGAACTTAGTCAAGTATCGGGAGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGATATCTTGGCACAGCACTTCGAAATCGTCGCTCGTTGTCAGGGTGGGGCTAATGCTGGGCACACAATCTATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATCCCTTCAGGGATCCTTAATGAAGGTACTTTATGTGTTGTTGGAAACGGAGTTGTGGTGCACGTGCCCGGTTTCTTCGAAGAAATCGACCGTCTAGAAGCGAGTGGAGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTTCATCAGGAGGTGGATGGCTTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGCAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGATACACTTCCTCAGAAGCTCGACCTTCTACTCTCCGATGCTGCGTCGAGATTCAAGGATTTTGTCTATACCCCAGAAATTCTGAAGGAAGAAGTCGAGAAGTACAAACGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGCATTTCATCAATGAAGCCATCTCTGATAATAAGAAAATTTTGGTTGAAGGTGGTCAAGCAACTATGCTTGACATCGACTTTGGTACTTATCCTTTCGTAACTTCTTCCAGCCCGTCCGCAGGTGGAATTTGCACCGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAAGGCATACACTACTAGAGTTGGTTCTGGCCCTTTTCCTACTGAGATCTTGGGAAAGGGGGGTGACCTCCTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGCCCTCGTCGCTGTGGATGGCTCGATATAGTTGCTCTAAAATATTGCTGTAAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTCGATGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTTTCTTATCGAGCACCTGATGGAACACCAGTCGAATCATTTCCCGCAGATCTTAGTCTTCTTGAACAAATAAAGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCAAAAGAATATGTAACAAGGATCGAAGAACTCGTGGGCATACCCGTGCATTACATCGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

Coding sequence (CDS)

ATGAGCATCTCTTCTCTTGCTTTCCAGCCCACTCTTTTCTCCGCCGCCAAATTGTCGTCGCCGGCCTGCCGGAGACCCTTTTCCGGACGTCATCCTGCACTCGTCGTTTGCTCCTCTGCTAAACCCGCGGCGGCGGTTAGTTCGTCTGTCAGTGCGCCGGAGTCAGCGGGGAAGGATGAGCTGAAACGAATTGGGGAACTTAGTCAAGTATCGGGAGTGTTGGGCTGCCAGTGGGGTGATGAAGGTAAAGGTAAACTCGTTGATATCTTGGCACAGCACTTCGAAATCGTCGCTCGTTGTCAGGGTGGGGCTAATGCTGGGCACACAATCTATAATGCAGAAGGGAAGAAGTTTGCTCTTCATCTCATCCCTTCAGGGATCCTTAATGAAGGTACTTTATGTGTTGTTGGAAACGGAGTTGTGGTGCACGTGCCCGGTTTCTTCGAAGAAATCGACCGTCTAGAAGCGAGTGGAGTCTCATGCAAGGGAAGGATCTTGGTGTCTGACCGAGCCCATCTGTTATTTGATTTTCATCAGGAGGTGGATGGCTTAAGAGAAGCTGAGTTAGCTAAATCTTTCATTGGAACAACCAGGAGAGGGATTGGACCTTGTTACTCTAGCAAAGTGATTAGAAATGGTATTAGAGTGGGTGATTTGAGGCACTTGGATACACTTCCTCAGAAGCTCGACCTTCTACTCTCCGATGCTGCGTCGAGATTCAAGGATTTTGTCTATACCCCAGAAATTCTGAAGGAAGAAGTCGAGAAGTACAAACGATATGCTGAGAGATTGGAACCATTTATCACTGACACTGTGCATTTCATCAATGAAGCCATCTCTGATAATAAGAAAATTTTGGTTGAAGGTGGTCAAGCAACTATGCTTGACATCGACTTTGGTACTTATCCTTTCGTAACTTCTTCCAGCCCGTCCGCAGGTGGAATTTGCACCGGTCTTGGAATTGCTCCAAAAGTTGTGGGTGATCTTATAGGAGTGGTGAAGGCATACACTACTAGAGTTGGTTCTGGCCCTTTTCCTACTGAGATCTTGGGAAAGGGGGGTGACCTCCTTAGGTTTGCTGGTCAAGAATTTGGTACCACAACTGGTCGCCCTCGTCGCTGTGGATGGCTCGATATAGTTGCTCTAAAATATTGCTGTAAAATTAATGGCTTCTCTTCTCTGAATCTTACGAAACTCGATGTTCTCTCAGAACTGTCTGAGATACAACTGGGGGTTTCTTATCGAGCACCTGATGGAACACCAGTCGAATCATTTCCCGCAGATCTTAGTCTTCTTGAACAAATAAAGGTGGAATATGAAACAATGCCTGGATGGATGTGTGATATCTCTTCAATAAGAGATTATTCTGACCTTCCTAAGGCTGCAAAAGAATATGTAACAAGGATCGAAGAACTCGTGGGCATACCCGTGCATTACATCGGTGTCGGTCCAGGCCGTGATGCACTATTATACAAATGA

Protein sequence

MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGTPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK
Homology
BLAST of CmoCh03G001000 vs. ExPASy Swiss-Prot
Match: B9SL58 (Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA2 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 6.1e-229
Identity = 384/462 (83.12%), Postives = 416/462 (90.04%), Query Frame = 0

Query: 31  HPALVVCSSAKPAAAVSSSVSAPESAGKDELKRIGELSQVSGVLGCQWGDEGKGKLVDIL 90
           H   VVCS   P  +  S   A +SA  D L RI  LSQVSGVLGCQWGDEGKGKLVDIL
Sbjct: 29  HRNFVVCSIKSP--STPSLNLATDSASTDSLSRINSLSQVSGVLGCQWGDEGKGKLVDIL 88

Query: 91  AQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEGTLCVVGNGVVVHVPGFFEE 150
           AQHF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE TLCV+GNGVVVH+PG F+E
Sbjct: 89  AQHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEDTLCVIGNGVVVHLPGLFKE 148

Query: 151 IDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVI 210
           ID LE+SGVSC GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCY+SK I
Sbjct: 149 IDGLESSGVSCTGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYASKAI 208

Query: 211 RNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFVYTPEILKEEVEKYKRYAERLEPFITD 270
           RNGIRV DLRH+DT PQKLD+LLSDAASRF+ F Y PE+L+EEVEKYKRYAERLEPFI D
Sbjct: 209 RNGIRVSDLRHMDTFPQKLDILLSDAASRFQGFKYGPEMLREEVEKYKRYAERLEPFIAD 268

Query: 271 TVHFINEAISDNKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLI 330
           TVH +NE+I   KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+ VGDLI
Sbjct: 269 TVHVMNESIEQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRAVGDLI 328

Query: 331 GVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCKING 390
           GVVKAYTTRVGSGPFPTEILG+GGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCC+ING
Sbjct: 329 GVVKAYTTRVGSGPFPTEILGQGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCQING 388

Query: 391 FSSLNLTKLDVLSELSEIQLGVSYRAPDGTPVESFPADLSLLEQIKVEYETMPGWMCDIS 450
           FSSLNLTKLDVLS+L EIQLGV+Y+  DGTP +SFPADL LLEQ+KVEYE +PGW  DIS
Sbjct: 389 FSSLNLTKLDVLSDLPEIQLGVAYKHADGTPFKSFPADLRLLEQLKVEYEVLPGWKSDIS 448

Query: 451 SIRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK 493
           SIR+Y+D PKAA++YV RIEELVG+PVHYIGVGPGRDAL+YK
Sbjct: 449 SIRNYADFPKAAQQYVERIEELVGVPVHYIGVGPGRDALIYK 488

BLAST of CmoCh03G001000 vs. ExPASy Swiss-Prot
Match: B9IJ21 (Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PURA PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.0e-228
Identity = 394/500 (78.80%), Postives = 436/500 (87.20%), Query Frame = 0

Query: 1   MSISSL--------AFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSA 60
           M++SSL        ++    F A    +P+ RR F       V CSS KP+A  SSS+S 
Sbjct: 1   MNLSSLRLESNPRWSYHAAHFPAHHGLNPSFRRNF-------VSCSSIKPSA--SSSLSV 60

Query: 61  PESAGKDELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYN 120
            ES  +D   RI  LSQVSGVLG QWGDEGKGKLVDILA+HF+IVARCQGGANAGHTIYN
Sbjct: 61  AESFTRDSASRIESLSQVSGVLGSQWGDEGKGKLVDILAEHFDIVARCQGGANAGHTIYN 120

Query: 121 AEGKKFALHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAH 180
           +EGKKFALHL+PSGILNE TLCV+GNGVVVH+PG F+EID LEA+GVSC GRILVSDRAH
Sbjct: 121 SEGKKFALHLVPSGILNEDTLCVIGNGVVVHLPGLFKEIDGLEANGVSCTGRILVSDRAH 180

Query: 181 LLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLL 240
           LLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRV DLRH+DT PQKLD L
Sbjct: 181 LLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVCDLRHMDTFPQKLDAL 240

Query: 241 LSDAASRFKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQA 300
           LSD ASRF+ F Y PE+LKEEVE+YKR+AERLEPFITDTVHF+NE+I+  KKILVEGGQA
Sbjct: 241 LSDVASRFESFKYGPEMLKEEVERYKRFAERLEPFITDTVHFMNESIAKKKKILVEGGQA 300

Query: 301 TMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGK 360
           TMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+VVGDLIGVVKAYT+RVGSGPFPTEILG+
Sbjct: 301 TMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLIGVVKAYTSRVGSGPFPTEILGQ 360

Query: 361 GGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGV 420
           GGDLLRFAGQEFGTTTGRPRRCGWLD+VALKY C+INGFSSLNLTKLDVLSE SEIQ+GV
Sbjct: 361 GGDLLRFAGQEFGTTTGRPRRCGWLDVVALKYVCQINGFSSLNLTKLDVLSEFSEIQIGV 420

Query: 421 SYRAPDGTPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEEL 480
           SY+  DGTPVESFP DL LLEQ+KV+YE +PGW  DISSIR Y+DLPKAA++YV RIEEL
Sbjct: 421 SYKQIDGTPVESFPGDLCLLEQLKVDYEVLPGWKSDISSIRKYADLPKAAQQYVERIEEL 480

Query: 481 VGIPVHYIGVGPGRDALLYK 493
           VG+P+HYIG+GPGRDAL+YK
Sbjct: 481 VGVPIHYIGIGPGRDALIYK 491

BLAST of CmoCh03G001000 vs. ExPASy Swiss-Prot
Match: Q96529 (Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PURA PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.8e-228
Identity = 389/493 (78.90%), Postives = 437/493 (88.64%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHP-ALVVCSSAKPAAAVSSSVSAPESAGKD 60
           MS+SSL        A  +  P  RR     HP + V CS+ +PA + S SV+A +SA  +
Sbjct: 1   MSLSSLTLDSNPRFA--VGGPYHRRYPPLHHPRSFVSCSAKRPAVSASLSVAA-DSAATE 60

Query: 61  ELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKFA
Sbjct: 61  SLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKFA 120

Query: 121 LHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHL+PSGILNE T CV+GNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDF 300
           F+ F YTPE+L+EEVE YKRYA+RLEP+ITDTVHFIN++IS  KK+LVEGGQATMLDIDF
Sbjct: 241 FQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR 
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLRL 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDG 420
           AGQEFGTTTGRPRRCGWLDIVALK+ C+INGF+SLNLTKLDVLS+L+EIQLGV+Y+  DG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSDG 420

Query: 421 TPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHY 480
           TPV+SFP DL LLE++ VEYE +PGW  DISS+R+YSDLPKAA++YV RIEELVG+P+HY
Sbjct: 421 TPVKSFPGDLRLLEELHVEYEVLPGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHY 480

Query: 481 IGVGPGRDALLYK 493
           IG+GPGRDAL+YK
Sbjct: 481 IGIGPGRDALIYK 490

BLAST of CmoCh03G001000 vs. ExPASy Swiss-Prot
Match: A9XLE1 (Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=PURA1 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 4.0e-228
Identity = 376/463 (81.21%), Postives = 424/463 (91.58%), Query Frame = 0

Query: 30  RHPALVVCSSAKPAAAVSSSVSAPESAGKDELKRIGELSQVSGVLGCQWGDEGKGKLVDI 89
           ++P+++VCS+ KP A+V       ES     L RI  LSQVSGVLGCQWGDEGKGKLVDI
Sbjct: 50  KNPSIIVCSTTKPLASVVDRHGVSESG----LSRIESLSQVSGVLGCQWGDEGKGKLVDI 109

Query: 90  LAQHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEGTLCVVGNGVVVHVPGFFE 149
           LA+HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE T+CV+GNGVVVH+PG F+
Sbjct: 110 LAKHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETVCVIGNGVVVHLPGLFK 169

Query: 150 EIDRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKV 209
           EID LE++GVSC+GRILVSDRAHLLFDFHQE+DGLREAELAKSFIGTT+RGIGPCYSSKV
Sbjct: 170 EIDGLESNGVSCQGRILVSDRAHLLFDFHQEIDGLREAELAKSFIGTTKRGIGPCYSSKV 229

Query: 210 IRNGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFVYTPEILKEEVEKYKRYAERLEPFIT 269
           IRNGIRV DLRH+DT PQKLD LLSDAA+RF  F Y PE+L+EEVE+YK++AERLEPFIT
Sbjct: 230 IRNGIRVSDLRHMDTFPQKLDRLLSDAAARFPGFKYGPEMLREEVERYKKFAERLEPFIT 289

Query: 270 DTVHFINEAISDNKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDL 329
           DTVHF+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+VVGDL
Sbjct: 290 DTVHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDL 349

Query: 330 IGVVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCKIN 389
           +GVVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL+YCC+IN
Sbjct: 350 VGVVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRYCCQIN 409

Query: 390 GFSSLNLTKLDVLSELSEIQLGVSYRAPDGTPVESFPADLSLLEQIKVEYETMPGWMCDI 449
           GF+SLNLTKLDVLS+LSEIQLGV+YR PDG+ + SFP+DL LLEQ+KVEYE + GW  DI
Sbjct: 410 GFASLNLTKLDVLSDLSEIQLGVTYRHPDGSILNSFPSDLRLLEQLKVEYEVLRGWQSDI 469

Query: 450 SSIRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK 493
           SSIR YSDLPK+A+EYV RIEELVG+P+HYIG+GPGRDAL+YK
Sbjct: 470 SSIRKYSDLPKSAREYVERIEELVGVPIHYIGIGPGRDALIYK 508

BLAST of CmoCh03G001000 vs. ExPASy Swiss-Prot
Match: Q6T7E8 (Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA PE=2 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.2e-227
Identity = 379/461 (82.21%), Postives = 424/461 (91.97%), Query Frame = 0

Query: 32  PALVVCSSAKPAAAVSSSVSAPESAGKDELKRIGELSQVSGVLGCQWGDEGKGKLVDILA 91
           P+++VCS+ KP A+V       +S     L RI  LSQVSGVLGCQWGDEGKGKLVDILA
Sbjct: 50  PSIIVCST-KPLASVVEHQGVNDSG----LTRIESLSQVSGVLGCQWGDEGKGKLVDILA 109

Query: 92  QHFEIVARCQGGANAGHTIYNAEGKKFALHLIPSGILNEGTLCVVGNGVVVHVPGFFEEI 151
           +HF+IVARCQGGANAGHTIYN+EGKKFALHL+PSGILNE T+CV+GNGVVVH+PG F+EI
Sbjct: 110 KHFDIVARCQGGANAGHTIYNSEGKKFALHLVPSGILNEETICVIGNGVVVHLPGLFKEI 169

Query: 152 DRLEASGVSCKGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTRRGIGPCYSSKVIR 211
           D LE++GVSC+GRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTT+RGIGPCYSSKVIR
Sbjct: 170 DNLESNGVSCQGRILVSDRAHLLFDFHQEVDGLREAELAKSFIGTTKRGIGPCYSSKVIR 229

Query: 212 NGIRVGDLRHLDTLPQKLDLLLSDAASRFKDFVYTPEILKEEVEKYKRYAERLEPFITDT 271
           NGIRV DLRH+DT PQ+LDLLLSDAASRF+ F Y  ++LKEEVE+YK++AERLEPFITDT
Sbjct: 230 NGIRVSDLRHMDTFPQQLDLLLSDAASRFQGFNYGRDMLKEEVERYKKFAERLEPFITDT 289

Query: 272 VHFINEAISDNKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPKVVGDLIG 331
           VHF+N+AIS  KKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAP+VVGDL+G
Sbjct: 290 VHFMNDAISQKKKILVEGGQATMLDIDFGTYPFVTSSSPSAGGICTGLGIAPRVVGDLVG 349

Query: 332 VVKAYTTRVGSGPFPTEILGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALKYCCKINGF 391
           VVKAYTTRVGSGPFPTEI+GKGGDLLRFAGQEFGTTTGRPRRCGWLDIVAL++CC+INGF
Sbjct: 350 VVKAYTTRVGSGPFPTEIMGKGGDLLRFAGQEFGTTTGRPRRCGWLDIVALRFCCQINGF 409

Query: 392 SSLNLTKLDVLSELSEIQLGVSYRAPDGTPVESFPADLSLLEQIKVEYETMPGWMCDISS 451
           +SLNLTKLDVLS+LSEIQLGV+YR PDG+ + SFP+D+ LLEQIKVEYE MPGW  DISS
Sbjct: 410 ASLNLTKLDVLSDLSEIQLGVTYRHPDGSTLNSFPSDIRLLEQIKVEYEVMPGWQSDISS 469

Query: 452 IRDYSDLPKAAKEYVTRIEELVGIPVHYIGVGPGRDALLYK 493
           +R YSDLPKAA+EYV RIEELVG+PVHYIGVGPGRDAL+YK
Sbjct: 470 VRKYSDLPKAAREYVERIEELVGVPVHYIGVGPGRDALIYK 505

BLAST of CmoCh03G001000 vs. ExPASy TrEMBL
Match: A0A6J1ELC2 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435468 PE=3 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 9.7e-286
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. ExPASy TrEMBL
Match: A0A6J1HXQ5 (Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467197 PE=3 SV=1)

HSP 1 Score: 981.5 bits (2536), Expect = 1.3e-282
Identity = 485/492 (98.58%), Postives = 488/492 (99.19%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKL SPACRRPFSGRHPAL+VCSSAKPAAAVSSSVSAPESAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVY+PEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYR PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. ExPASy TrEMBL
Match: A0A0A0L1E1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.3e-269
Identity = 464/493 (94.12%), Postives = 476/493 (96.55%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLS-SPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKD 60
           M+ISSLAF+PTLFSAA LS S ACRRPFSGRHP L+VCSSAK  AAVSSSVS PES  KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFSGRHPPLIVCSSAKTVAAVSSSVSVPESGRKD 60

Query: 61  ELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDF 300
           FKDF YTPE+LKEEVE YKRYAERLEPFITDTV+FINEAIS NK+ILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLKEEVENYKRYAERLEPFITDTVYFINEAISANKRILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLGVSY  PDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHY 480
           TPV SFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEY++RIEELVG+PVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGLPVHY 480

Query: 481 IGVGPGRDALLYK 493
           IGVGPGRDALLYK
Sbjct: 481 IGVGPGRDALLYK 493

BLAST of CmoCh03G001000 vs. ExPASy TrEMBL
Match: A0A5A7UY81 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=PURA PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.1e-268
Identity = 463/493 (93.91%), Postives = 474/493 (96.15%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLS-SPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKD 60
           M+ISSLAF+PTLFSAA LS S ACRRPF GR+P L VCSSAK  AAV SSVS PES  KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  ELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLGVSY  PDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHY 480
           TPV SFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEY++RIEELVGIPVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHY 480

Query: 481 IGVGPGRDALLYK 493
           IGVGPGRDALLYK
Sbjct: 481 IGVGPGRDALLYK 493

BLAST of CmoCh03G001000 vs. ExPASy TrEMBL
Match: A0A1S3B4T1 (Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486156 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.1e-268
Identity = 463/493 (93.91%), Postives = 474/493 (96.15%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLS-SPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKD 60
           M+ISSLAF+PTLFSAA LS S ACRRPF GR+P L VCSSAK  AAV SSVS PES  KD
Sbjct: 1   MNISSLAFEPTLFSAANLSPSSACRRPFYGRYPTLFVCSSAKTVAAVGSSVSVPESGRKD 60

Query: 61  ELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA
Sbjct: 61  GLKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120

Query: 121 LHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDF 300
           FKDF YTPE+L+EEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDF
Sbjct: 241 FKDFNYTPEVLEEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDG 420
           AGQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQLGVSY  PDG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQLGVSYSTPDG 420

Query: 421 TPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHY 480
           TPV SFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEY++RIEELVGIPVHY
Sbjct: 421 TPVRSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYISRIEELVGIPVHY 480

Query: 481 IGVGPGRDALLYK 493
           IGVGPGRDALLYK
Sbjct: 481 IGVGPGRDALLYK 493

BLAST of CmoCh03G001000 vs. NCBI nr
Match: XP_022928609.1 (adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 991.9 bits (2563), Expect = 2.0e-285
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. NCBI nr
Match: KAG6603253.1 (Adenylosuccinate synthetase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037386.1 Adenylosuccinate synthetase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 988.0 bits (2553), Expect = 2.9e-284
Identity = 490/492 (99.59%), Postives = 490/492 (99.59%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYR PDG 
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGM 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. NCBI nr
Match: XP_022967784.1 (adenylosuccinate synthetase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 981.5 bits (2536), Expect = 2.7e-282
Identity = 485/492 (98.58%), Postives = 488/492 (99.19%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKL SPACRRPFSGRHPAL+VCSSAKPAAAVSSSVSAPESAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHPALIVCSSAKPAAAVSSSVSAPESAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLRE ELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLP KLDLLLSDAASRF
Sbjct: 181 VDGLREVELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPHKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVY+PEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYSPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYR PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. NCBI nr
Match: XP_023543557.1 (adenylosuccinate synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974.5 bits (2518), Expect = 3.3e-280
Identity = 482/492 (97.97%), Postives = 485/492 (98.58%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           MSISSLAFQPTLFSAAKL SPACRRPFSGRH  LVVCSSAKP  AVSSSV+ P+SAGKDE
Sbjct: 1   MSISSLAFQPTLFSAAKLPSPACRRPFSGRHHTLVVCSSAKPPVAVSSSVNVPQSAGKDE 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG
Sbjct: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYR PDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRTPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYV+RIEELVGIPVHYI
Sbjct: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVSRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. NCBI nr
Match: XP_038882112.1 (LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispida])

HSP 1 Score: 945.3 bits (2442), Expect = 2.1e-271
Identity = 467/492 (94.92%), Postives = 478/492 (97.15%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHPALVVCSSAKPAAAVSSSVSAPESAGKDE 60
           M+ISSLAF+PTLFSAA   SPACRRP S RHPALVVCSS KPAAAVSSSVS P+SA KD 
Sbjct: 1   MNISSLAFEPTLFSAANXPSPACRRPXSARHPALVVCSSTKPAAAVSSSVSVPDSARKDG 60

Query: 61  LKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120
           LKRIGELSQV GVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL
Sbjct: 61  LKRIGELSQVXGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFAL 120

Query: 121 HLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180
           HLIPSGILNE TLCV+GNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE
Sbjct: 121 HLIPSGILNEDTLCVIGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQE 180

Query: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240
           VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF
Sbjct: 181 VDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASRF 240

Query: 241 KDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDFG 300
           KDF YTPEILKEEVEKYKRYAERLEPFITDTV+FINEAIS NKKILVEGGQATMLDIDFG
Sbjct: 241 KDFNYTPEILKEEVEKYKRYAERLEPFITDTVYFINEAISANKKILVEGGQATMLDIDFG 300

Query: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360
           TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA
Sbjct: 301 TYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRFA 360

Query: 361 GQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDGT 420
           GQEFGTTTGRPRRCGWLDIVALKYCC+INGFSSLNLTKLDVLSELSEIQ+GVSY APDGT
Sbjct: 361 GQEFGTTTGRPRRCGWLDIVALKYCCQINGFSSLNLTKLDVLSELSEIQVGVSYWAPDGT 420

Query: 421 PVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHYI 480
           PV+SFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAK+Y++RIEELVGIPVHYI
Sbjct: 421 PVKSFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKDYISRIEELVGIPVHYI 480

Query: 481 GVGPGRDALLYK 493
           GVGPGRDALLYK
Sbjct: 481 GVGPGRDALLYK 492

BLAST of CmoCh03G001000 vs. TAIR 10
Match: AT3G57610.1 (adenylosuccinate synthase )

HSP 1 Score: 793.1 bits (2047), Expect = 1.3e-229
Identity = 389/493 (78.90%), Postives = 437/493 (88.64%), Query Frame = 0

Query: 1   MSISSLAFQPTLFSAAKLSSPACRRPFSGRHP-ALVVCSSAKPAAAVSSSVSAPESAGKD 60
           MS+SSL        A  +  P  RR     HP + V CS+ +PA + S SV+A +SA  +
Sbjct: 1   MSLSSLTLDSNPRFA--VGGPYHRRYPPLHHPRSFVSCSAKRPAVSASLSVAA-DSAATE 60

Query: 61  ELKRIGELSQVSGVLGCQWGDEGKGKLVDILAQHFEIVARCQGGANAGHTIYNAEGKKFA 120
            L RIG LSQVSGVLGCQWGDEGKGKLVDILAQHF+IVARCQGGANAGHTIYN+EGKKFA
Sbjct: 61  SLGRIGSLSQVSGVLGCQWGDEGKGKLVDILAQHFDIVARCQGGANAGHTIYNSEGKKFA 120

Query: 121 LHLIPSGILNEGTLCVVGNGVVVHVPGFFEEIDRLEASGVSCKGRILVSDRAHLLFDFHQ 180
           LHL+PSGILNE T CV+GNGVVVH+PG F+EID LE++GVSCKGRILVSDRAHLLFDFHQ
Sbjct: 121 LHLVPSGILNEDTTCVIGNGVVVHLPGLFKEIDGLESNGVSCKGRILVSDRAHLLFDFHQ 180

Query: 181 EVDGLREAELAKSFIGTTRRGIGPCYSSKVIRNGIRVGDLRHLDTLPQKLDLLLSDAASR 240
           EVDGLRE+ELAKSFIGTT+RGIGP YSSKVIRNGIRVGDLRH+DTLPQKLDLLLSDAA+R
Sbjct: 181 EVDGLRESELAKSFIGTTKRGIGPAYSSKVIRNGIRVGDLRHMDTLPQKLDLLLSDAAAR 240

Query: 241 FKDFVYTPEILKEEVEKYKRYAERLEPFITDTVHFINEAISDNKKILVEGGQATMLDIDF 300
           F+ F YTPE+L+EEVE YKRYA+RLEP+ITDTVHFIN++IS  KK+LVEGGQATMLDIDF
Sbjct: 241 FQGFKYTPEMLREEVEAYKRYADRLEPYITDTVHFINDSISQKKKVLVEGGQATMLDIDF 300

Query: 301 GTYPFVTSSSPSAGGICTGLGIAPKVVGDLIGVVKAYTTRVGSGPFPTEILGKGGDLLRF 360
           GTYPFVTSSSPSAGGICTGLGIAP VVGDLIGVVKAYTTRVGSGPFPTE LG GGDLLR 
Sbjct: 301 GTYPFVTSSSPSAGGICTGLGIAPSVVGDLIGVVKAYTTRVGSGPFPTENLGTGGDLLRL 360

Query: 361 AGQEFGTTTGRPRRCGWLDIVALKYCCKINGFSSLNLTKLDVLSELSEIQLGVSYRAPDG 420
           AGQEFGTTTGRPRRCGWLDIVALK+ C+INGF+SLNLTKLDVLS+L+EIQLGV+Y+  DG
Sbjct: 361 AGQEFGTTTGRPRRCGWLDIVALKFSCQINGFASLNLTKLDVLSDLNEIQLGVAYKRSDG 420

Query: 421 TPVESFPADLSLLEQIKVEYETMPGWMCDISSIRDYSDLPKAAKEYVTRIEELVGIPVHY 480
           TPV+SFP DL LLE++ VEYE +PGW  DISS+R+YSDLPKAA++YV RIEELVG+P+HY
Sbjct: 421 TPVKSFPGDLRLLEELHVEYEVLPGWKSDISSVRNYSDLPKAAQQYVERIEELVGVPIHY 480

Query: 481 IGVGPGRDALLYK 493
           IG+GPGRDAL+YK
Sbjct: 481 IGIGPGRDALIYK 490

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B9SL586.1e-22983.12Adenylosuccinate synthetase 2, chloroplastic OS=Ricinus communis OX=3988 GN=PURA... [more]
B9IJ211.0e-22878.80Adenylosuccinate synthetase, chloroplastic OS=Populus trichocarpa OX=3694 GN=PUR... [more]
Q965291.8e-22878.90Adenylosuccinate synthetase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU... [more]
A9XLE14.0e-22881.21Adenylosuccinate synthetase 1, chloroplastic OS=Capsicum frutescens OX=4073 GN=P... [more]
Q6T7E81.2e-22782.21Adenylosuccinate synthetase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=PURA ... [more]
Match NameE-valueIdentityDescription
A0A6J1ELC29.7e-286100.00Adenylosuccinate synthetase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HXQ51.3e-28298.58Adenylosuccinate synthetase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0L1E11.3e-26994.12Adenylosuccinate synthetase, chloroplastic OS=Cucumis sativus OX=3659 GN=PURA PE... [more]
A0A5A7UY814.1e-26893.91Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B4T14.1e-26893.91Adenylosuccinate synthetase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034861... [more]
Match NameE-valueIdentityDescription
XP_022928609.12.0e-285100.00adenylosuccinate synthetase, chloroplastic-like [Cucurbita moschata][more]
KAG6603253.12.9e-28499.59Adenylosuccinate synthetase, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_022967784.12.7e-28298.58adenylosuccinate synthetase, chloroplastic [Cucurbita maxima][more]
XP_023543557.13.3e-28097.97adenylosuccinate synthetase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_038882112.12.1e-27194.92LOW QUALITY PROTEIN: adenylosuccinate synthetase, chloroplastic [Benincasa hispi... [more]
Match NameE-valueIdentityDescription
AT3G57610.11.3e-22978.90adenylosuccinate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001114Adenylosuccinate synthetaseSMARTSM00788adenylsucc_syntcoord: 70..491
e-value: 1.9E-245
score: 831.4
IPR001114Adenylosuccinate synthetasePFAMPF00709Adenylsucc_syntcoord: 73..488
e-value: 2.6E-171
score: 570.2
IPR001114Adenylosuccinate synthetaseTIGRFAMTIGR00184TIGR00184coord: 73..488
e-value: 1.0E-155
score: 516.8
IPR001114Adenylosuccinate synthetasePANTHERPTHR11846ADENYLOSUCCINATE SYNTHETASEcoord: 30..492
IPR001114Adenylosuccinate synthetaseHAMAPMF_00011Adenylosucc_synthcoord: 1..492
score: 176.594482
IPR001114Adenylosuccinate synthetaseCDDcd03108AdSScoord: 69..491
e-value: 5.28619E-180
score: 505.111
IPR042111Adenylosuccinate synthetase, domain 3GENE3D3.90.170.10Adenylosuccinate Synthetase, subunit A, domain 3coord: 333..492
e-value: 3.3E-69
score: 233.6
IPR042110Adenylosuccinate synthetase, domain 2GENE3D1.10.300.10Adenylosuccinate Synthetase, subunit A, domain 2coord: 169..267
e-value: 6.9E-111
score: 372.1
IPR042109Adenylosuccinate synthetase, domain 1GENE3D3.40.440.10Adenylosuccinate Synthetase, subunit A, domain 1coord: 73..332
e-value: 6.9E-111
score: 372.1
NoneNo IPR availablePANTHERPTHR11846:SF14ADENYLOSUCCINATE SYNTHETASE, CHLOROPLASTICcoord: 30..492
IPR018220Adenylosuccinate synthase, GTP-binding sitePROSITEPS01266ADENYLOSUCCIN_SYN_1coord: 77..84
IPR033128Adenylosuccinate synthase, active sitePROSITEPS00513ADENYLOSUCCIN_SYN_2coord: 200..211
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 69..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G001000.1CmoCh03G001000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044208 'de novo' AMP biosynthetic process
biological_process GO:0046040 IMP metabolic process
biological_process GO:0006164 purine nucleotide biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004019 adenylosuccinate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0000287 magnesium ion binding