CmoCh03G000790 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh03G000790
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionpolygalacturonase inhibitor-like
LocationCmo_Chr03: 1214437 .. 1215423 (-)
RNA-Seq ExpressionCmoCh03G000790
SyntenyCmoCh03G000790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTTCTCCATCCACCTTCCTCTTCATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAGCTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACTTGGTACTGCGTCGAATGTGATCGGAAGTCCCACCGGATCGTGTCTCTCACCATCTTCGCCGACACGAGGCTCTCGGGTCAAATCCCACCGTTCGTCGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACAGTCTAACTGGTTCAATCCCAAACTTCCTCAGCTCCCTCACCAATCTCGATCTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTCGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCTTATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTGGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTACTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGGAAATTCCTGTGATGGCAGTTAACTTGCAGTTGCAGCTGCTGAATGTTAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAGAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

mRNA sequence

ATGTCTTCTTCTCCATCCACCTTCCTCTTCATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAGCTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACTTGGTACTGCGTCGAATGTGATCGGAAGTCCCACCGGATCGTGTCTCTCACCATCTTCGCCGACACGAGGCTCTCGGGTCAAATCCCACCGTTCGTCGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACAGTCTAACTGGTTCAATCCCAAACTTCCTCAGCTCCCTCACCAATCTCGATCTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTCGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCTTATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTGGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTACTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGGAAATTCCTGTGATGGCAGTTAACTTGCAGTTGCAGCTGCTGAATGTTAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAGAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

Coding sequence (CDS)

ATGTCTTCTTCTCCATCCACCTTCCTCTTCATCTTCTTCCTATTCTTCACCATTTCATTTGCAGAGCTCTGCCATCCAAATGACAAGAAAGCCCTTCTCAACATCAAAAAAGCCTTCAACAATCCCTACATTCTCACCTCATGGAAGCCTGAAGAAGATTGTTGCACTTGGTACTGCGTCGAATGTGATCGGAAGTCCCACCGGATCGTGTCTCTCACCATCTTCGCCGACACGAGGCTCTCGGGTCAAATCCCACCGTTCGTCGGCGACCTCCCGTACCTCGAAAACCTCATGTTCCACAAGCTTCCAAATCTCACTGGCCCCATCCAACCCGCCATTGCCAAACTCCATAACCTAAAATACCTTGACCTAAGTTGGAACAGTCTAACTGGTTCAATCCCAAACTTCCTCAGCTCCCTCACCAATCTCGATCTTCTCGACCTCTCTTTCAACCGACTCACCGGCTCCATCCCGAGCTCCCTCGCCAACCTCCAACGCCTCGGCACTCTCCATTTAGACCGAAACAAATTAACCGGACCGATCCCCGATTCATTCGGAAACTTCAAGGGAAAAATCCCTTATCTTTATCTTTCACACAACCAACTCTCTGGGAAAATTCCAACTTCCTTGGAGAGAGTGGATTTCAATGTGATTGATTTGTCGAGGAACAGGCTCGAGGGCGATGGATCGGTGATATTCGGAGGGAAGAAGACGACGGAGATCGTCGACTTGTCGAGGAACTTGTTGGAGTTCGACATGTCGAAGGTCGTACTTCCTCGGAGCTTGACTTGGTTGGATCTTAACCATAATAAGATCTTCGGGGAAATTCCTGTGATGGCAGTTAACTTGCAGTTGCAGCTGCTGAATGTTAGTTACAACAAGCTTTGTGGTCGGATTCCGGTCGGAGGGAAGTTGCAGAGCTTCGATGTCTACTCCTATTCTAATAACAAGTGCTTGTGTGGAAAGCCACTTGGTAAATGCAAATGA

Protein sequence

MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK
Homology
BLAST of CmoCh03G000790 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-124
Identity = 220/326 (67.48%), Postives = 254/326 (77.91%), Query Frame = 0

Query: 6   STFLFIFFLFFTI---SFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 65
           STFL +  LF ++   + ++LC+P+DKK LL IKKAF +PY+L SWK + DCC WYCV C
Sbjct: 6   STFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTC 65

Query: 66  DRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYL 125
           D  ++RI SLTIFA  ++SGQIP  VGDLPYLE L FHK PNLTGPIQPAIAKL  LK L
Sbjct: 66  DSTTNRINSLTIFAG-QVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSL 125

Query: 126 DLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIP 185
            LSW +L+GS+P+FLS L NL  LDLSFN LTG+IPSSL+ L  LG L LDRNKLTG IP
Sbjct: 126 RLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIP 185

Query: 186 DSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIV 245
            SFG F G +P LYLSHNQLSG IPTS  ++DF  IDLSRN+LEGD SVIFG  KTT+IV
Sbjct: 186 ISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIV 245

Query: 246 DLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVG 305
           DLSRNLLEF++SKV  P SLT LD+NHNKI+G IPV    L  Q LNVSYN+LCG+IPVG
Sbjct: 246 DLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVG 305

Query: 306 GKLQSFDVYSYSNNKCLCGKPLGKCK 329
           GKLQSFD YSY +N+CLCG PL  CK
Sbjct: 306 GKLQSFDEYSYFHNRCLCGAPLPSCK 330

BLAST of CmoCh03G000790 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.7e-116
Identity = 202/310 (65.16%), Postives = 234/310 (75.48%), Query Frame = 0

Query: 19  SFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHRIVSLTIFADT 78
           S +E C+P DKK LL IKKA +NPYIL SW P  DCC WYCVECD  +HRI SLTIF+  
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFSG- 84

Query: 79  RLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNSLTGSIPNFLS 138
           +LSGQIP  VGDLP+LE L+F KL NLTG I PAIAKL +LK + LSW +L+G +P F S
Sbjct: 85  QLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFS 144

Query: 139 SLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNFKGKIPYLYLS 198
            L NL  LDLSFN L+G IP SL+ L  LG LHLDRN LTGPIPDSFG F G  P L+LS
Sbjct: 145 ELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGSTPGLHLS 204

Query: 199 HNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNLLEFDMSKVVL 258
           HNQLSGKIP S    D NV+DLSRN+LEGD S+ F   K+T+IVD SRNL +FD+S+V  
Sbjct: 205 HNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVEF 264

Query: 259 PRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKC 318
           P+SLT LDL+HNKI G +P M  +L LQ LNVSYN+LCG+IPVGGKLQSFD  SY +N+C
Sbjct: 265 PKSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNRC 324

Query: 319 LCGKPLGKCK 329
           LCG PL  CK
Sbjct: 325 LCGAPLQSCK 333

BLAST of CmoCh03G000790 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 9.1e-112
Identity = 197/322 (61.18%), Postives = 238/322 (73.91%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECD--RKS 68
           LF+F    T    +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC+EC     +
Sbjct: 10  LFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATVN 69

Query: 69  HRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSW 128
           HR+ +LTIF+  ++SGQIP  VGDLPYLE L+F KL NLTG IQP IAKL NL+ L LSW
Sbjct: 70  HRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLSW 129

Query: 129 NSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFG 188
            +LTG IP+F+S L NL+ L+LSFN L+GSIPSSL+ L ++  L L RNKLTG IP+SFG
Sbjct: 130 TNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESFG 189

Query: 189 NFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSR 248
           +F G +P L LSHNQLSG IP SL  +DFN IDLSRN+L+GD S++FG  KTT  +DLSR
Sbjct: 190 SFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLSR 249

Query: 249 NLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQ 308
           N+ +FD+SKV +P++L  LDLNHN I G IPV      LQ  NVSYNKLCG IP GGKLQ
Sbjct: 250 NMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGGKLQ 309

Query: 309 SFDVYSYSNNKCLCGKPLGKCK 329
           +FD YSY +NKCLCG PL  CK
Sbjct: 310 TFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmoCh03G000790 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 402.9 bits (1034), Expect = 3.5e-111
Identity = 199/331 (60.12%), Postives = 241/331 (72.81%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + + FL +  L  T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTMTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYC 60

Query: 61  VECD--RKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ SL I  D  +SGQIPP VGDLPYL +L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLK 120

Query: 121 NLKYLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL +L LSW +LTG +P FLS L NL+ +DLSFN L+GSIPSSL++L++L  L L RNKL
Sbjct: 121 NLTFLRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TGPIP+SFG F GK+P L+LSHNQLSG IP SL   DF  IDLSRN+L+GD S++FG KK
Sbjct: 181 TGPIPESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCG 300
           TT IVD+SRN+ +FD+SKV L ++L  LD+NHN I G IP        QLLNVSYN+LCG
Sbjct: 241 TTWIVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           RIP G  +Q FD YS+ +NKCLCG PL  CK
Sbjct: 301 RIPKGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmoCh03G000790 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 6.8e-83
Identity = 160/320 (50.00%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCT-WYCVECDRKSH 68
           +F   LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCCT W  VEC  +  
Sbjct: 9   IFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVECTNR-- 68

Query: 69  RIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWN 128
           R+  L++     +SGQI   +GDL  L  L F  LP+LTG I   I KL NL  L L   
Sbjct: 69  RVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHT 128

Query: 129 SLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGN 188
           SL+G IP+++S L +L  LDLSFN+ TG IP SL+ + +L  + ++ NKLTG IP+SFG+
Sbjct: 129 SLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGS 188

Query: 189 FKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRN 248
           F G +P LYLS+N+LSGKIP SL + DFN +DLS N  EGD  + FG  KTT  VDLSRN
Sbjct: 189 FVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRN 248

Query: 249 LLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQS 308
           +  FD+ KV   RS+  LDL+ N I+G+IP     L L+  NVS N LCG+IP GG LQ+
Sbjct: 249 MFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQT 308

Query: 309 FDVYSYSNNKCLCGKPLGKC 328
           F+  ++++N CLCG PL  C
Sbjct: 309 FEPSAFAHNICLCGTPLKAC 325

BLAST of CmoCh03G000790 vs. ExPASy TrEMBL
Match: A0A6J1ELI8 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.1e-192
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmoCh03G000790 vs. ExPASy TrEMBL
Match: A0A6J1HW96 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.3e-189
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFL IFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDR SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWN+LTGSIPNFLSSLTNL+LLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTS ERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFG+IPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmoCh03G000790 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.1e-163
Identity = 271/320 (84.69%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHR 68
           L + F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD KSHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNS 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NLD+LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS+ +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFGEIP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmoCh03G000790 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.1e-163
Identity = 271/320 (84.69%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHR 68
           L + F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD KSHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNS 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NLD+LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS+ +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFGEIP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmoCh03G000790 vs. ExPASy TrEMBL
Match: K9MZG2 (Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 GN=pgip PE=4 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 6.9e-163
Identity = 271/320 (84.69%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHR 68
           L + F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD KSHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNS 128
           I +LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  ITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NLD+LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS+ +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFGEIP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmoCh03G000790 vs. NCBI nr
Match: XP_022928674.1 (polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 680.2 bits (1754), Expect = 8.6e-192
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmoCh03G000790 vs. NCBI nr
Match: KAG6603233.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 677.9 bits (1748), Expect = 4.2e-191
Identity = 326/328 (99.39%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           +CDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  KCDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWNSLTGSIPNFLSSLTNLD+LDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNSLTGSIPNFLSSLTNLDILDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmoCh03G000790 vs. NCBI nr
Match: XP_022967838.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 670.2 bits (1728), Expect = 8.9e-189
Identity = 322/328 (98.17%), Postives = 325/328 (99.09%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60
           MSSSPSTFL IFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV
Sbjct: 1   MSSSPSTFLLIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCV 60

Query: 61  ECDRKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120
           ECDR SHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK
Sbjct: 61  ECDRNSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLK 120

Query: 121 YLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180
           YLDLSWN+LTGSIPNFLSSLTNL+LLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP
Sbjct: 121 YLDLSWNTLTGSIPNFLSSLTNLELLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGP 180

Query: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240
           IPDSFGNFKGKIPYLYLSHNQLSGKIPTS ERVDFNVIDLSRNRLEGDGSVIFGGKKTTE
Sbjct: 181 IPDSFGNFKGKIPYLYLSHNQLSGKIPTSFERVDFNVIDLSRNRLEGDGSVIFGGKKTTE 240

Query: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIP 300
           IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFG+IPVMAVNLQLQLLNVSYNKLCGRIP
Sbjct: 241 IVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGKIPVMAVNLQLQLLNVSYNKLCGRIP 300

Query: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           VGGKLQSFDVYSYSNNKCLCGKPLGKCK
Sbjct: 301 VGGKLQSFDVYSYSNNKCLCGKPLGKCK 328

BLAST of CmoCh03G000790 vs. NCBI nr
Match: NP_001303611.1 (polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalacturonase-inhibiting protein [Cucumis melo] >KAA0053950.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa] >TYK04261.1 polygalacturonase inhibitor-like precursor [Cucumis melo var. makuwa])

HSP 1 Score: 584.3 bits (1505), Expect = 6.4e-163
Identity = 271/320 (84.69%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHR 68
           L + F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD KSHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNS 128
           I++LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  IIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NLD+LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS+ +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFGEIP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmoCh03G000790 vs. NCBI nr
Match: AFY10521.1 (polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis])

HSP 1 Score: 583.2 bits (1502), Expect = 1.4e-162
Identity = 271/320 (84.69%), Postives = 293/320 (91.56%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECDRKSHR 68
           L + F FFT+SFAELCHPNDK+ LLNIKKAFNNPYILTSWKPEEDCCTWYCVECD KSHR
Sbjct: 7   LLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSHR 66

Query: 69  IVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWNS 128
           I +LTIFAD  LSG IPPFVGDLP+LENLMFHKLPNLTGPI P IAKLHNLKYLDLSWN 
Sbjct: 67  ITALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWNG 126

Query: 129 LTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGNF 188
           L+G IP+FL SL+NLD+LDLSFNR TGSIPSSLANL+RLGTLHLDRNKLTGPIPDSFGNF
Sbjct: 127 LSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGNF 186

Query: 189 KGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRNL 248
           KGK+PYLYLSHNQLSGKIPTS+ +VDFN IDLSRN+L GDGS+IFG KKTTEIVDLSRNL
Sbjct: 187 KGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRNL 246

Query: 249 LEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQSF 308
           LEF++SKVV PR+LTWLDLNHNKIFGEIP   V L+LQ+LNVSYN LCGRIP+GGKLQSF
Sbjct: 247 LEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKLELQMLNVSYNALCGRIPMGGKLQSF 306

Query: 309 DVYSYSNNKCLCGKPLGKCK 329
           DVYSY +NKCLCGKPLG CK
Sbjct: 307 DVYSYFHNKCLCGKPLGSCK 326

BLAST of CmoCh03G000790 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 404.8 bits (1039), Expect = 6.5e-113
Identity = 197/322 (61.18%), Postives = 238/322 (73.91%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYCVECD--RKS 68
           LF+F    T    +LC+ NDK  LL IKK+ NNPY L SW P+ DCC+WYC+EC     +
Sbjct: 10  LFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATVN 69

Query: 69  HRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSW 128
           HR+ +LTIF+  ++SGQIP  VGDLPYLE L+F KL NLTG IQP IAKL NL+ L LSW
Sbjct: 70  HRVTALTIFSG-QISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLSW 129

Query: 129 NSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFG 188
            +LTG IP+F+S L NL+ L+LSFN L+GSIPSSL+ L ++  L L RNKLTG IP+SFG
Sbjct: 130 TNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESFG 189

Query: 189 NFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSR 248
           +F G +P L LSHNQLSG IP SL  +DFN IDLSRN+L+GD S++FG  KTT  +DLSR
Sbjct: 190 SFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLSR 249

Query: 249 NLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQ 308
           N+ +FD+SKV +P++L  LDLNHN I G IPV      LQ  NVSYNKLCG IP GGKLQ
Sbjct: 250 NMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGGKLQ 309

Query: 309 SFDVYSYSNNKCLCGKPLGKCK 329
           +FD YSY +NKCLCG PL  CK
Sbjct: 310 TFDSYSYFHNKCLCGAPLEICK 330

BLAST of CmoCh03G000790 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 402.9 bits (1034), Expect = 2.5e-112
Identity = 199/331 (60.12%), Postives = 241/331 (72.81%), Query Frame = 0

Query: 1   MSSSPSTFLFIFFLFFTISFA-ELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCTWYC 60
           M  + + FL +  L  T S A +LCH +DK  LL IKK+ NNPY L SW P+ DCC+WYC
Sbjct: 1   MDKTMTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYC 60

Query: 61  VECD--RKSHRIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLH 120
           +EC     +HR+ SL I  D  +SGQIPP VGDLPYL +L+F KL NLTG IQP IAKL 
Sbjct: 61  LECGDATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLK 120

Query: 121 NLKYLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKL 180
           NL +L LSW +LTG +P FLS L NL+ +DLSFN L+GSIPSSL++L++L  L L RNKL
Sbjct: 121 NLTFLRLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKL 180

Query: 181 TGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKK 240
           TGPIP+SFG F GK+P L+LSHNQLSG IP SL   DF  IDLSRN+L+GD S++FG KK
Sbjct: 181 TGPIPESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKK 240

Query: 241 TTEIVDLSRNLLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCG 300
           TT IVD+SRN+ +FD+SKV L ++L  LD+NHN I G IP        QLLNVSYN+LCG
Sbjct: 241 TTWIVDISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCG 300

Query: 301 RIPVGGKLQSFDVYSYSNNKCLCGKPLGKCK 329
           RIP G  +Q FD YS+ +NKCLCG PL  CK
Sbjct: 301 RIPKGEYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of CmoCh03G000790 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 308.9 bits (790), Expect = 4.8e-84
Identity = 160/320 (50.00%), Postives = 206/320 (64.38%), Query Frame = 0

Query: 9   LFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPYILTSWKPEEDCCT-WYCVECDRKSH 68
           +F   LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCCT W  VEC  +  
Sbjct: 9   IFFSILFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVECTNR-- 68

Query: 69  RIVSLTIFADTRLSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKLHNLKYLDLSWN 128
           R+  L++     +SGQI   +GDL  L  L F  LP+LTG I   I KL NL  L L   
Sbjct: 69  RVTGLSV-TSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHT 128

Query: 129 SLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNKLTGPIPDSFGN 188
           SL+G IP+++S L +L  LDLSFN+ TG IP SL+ + +L  + ++ NKLTG IP+SFG+
Sbjct: 129 SLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGS 188

Query: 189 FKGKIPYLYLSHNQLSGKIPTSLERVDFNVIDLSRNRLEGDGSVIFGGKKTTEIVDLSRN 248
           F G +P LYLS+N+LSGKIP SL + DFN +DLS N  EGD  + FG  KTT  VDLSRN
Sbjct: 189 FVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRN 248

Query: 249 LLEFDMSKVVLPRSLTWLDLNHNKIFGEIPVMAVNLQLQLLNVSYNKLCGRIPVGGKLQS 308
           +  FD+ KV   RS+  LDL+ N I+G+IP     L L+  NVS N LCG+IP GG LQ+
Sbjct: 249 MFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQT 308

Query: 309 FDVYSYSNNKCLCGKPLGKC 328
           F+  ++++N CLCG PL  C
Sbjct: 309 FEPSAFAHNICLCGTPLKAC 325

BLAST of CmoCh03G000790 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 188.7 bits (478), Expect = 7.3e-48
Identity = 120/364 (32.97%), Postives = 181/364 (49.73%), Query Frame = 0

Query: 3   SSPSTFLFIFFLFFTISFAELCHPNDKKALLNIKKAFNNPY--ILTSWKPEEDCC-TWYC 62
           +SP + L        IS    C P D+ AL   K + + P   I  +W    DCC  WY 
Sbjct: 6   ASPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYG 65

Query: 63  VECDRKSHRIVSLTIFADTR------------LSGQIPPFVGDLPYLENLMFHKLPNLTG 122
           + CD  S R+  +++  ++             +SG I P V DL  L +L+      +TG
Sbjct: 66  ISCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITG 125

Query: 123 PIQPAIAKLHNLKYLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRL 182
            I P I  L +L+ LDL+ N +TG IP  +  L+ L +L+L+ N+++G IP+SL +L  L
Sbjct: 126 EIPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIEL 185

Query: 183 GTLHLDRNKLTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERVD-FNVIDLSRNRLE 242
             L L  N +TG IP  FG+ K  +  + L  N+L+G IP S+  ++    +DLS+N +E
Sbjct: 186 KHLELTENGITGVIPADFGSLK-MLSRVLLGRNELTGSIPESISGMERLADLDLSKNHIE 245

Query: 243 GDGSVIFGGKKT------------------------TEIVDLSRNLLEFDMSKVVLPRS- 302
           G      G  K                          ++ +LSRN LE  +  V   ++ 
Sbjct: 246 GPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTY 305

Query: 303 LTWLDLNHNKIFGEIPVMAVNLQ-LQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLC 325
           L  LDL+HN + G IP    + + +  L++S+NKLCGRIP G      +  S+S+N+CLC
Sbjct: 306 LVSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLC 365

BLAST of CmoCh03G000790 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 179.9 bits (455), Expect = 3.4e-45
Identity = 118/357 (33.05%), Postives = 177/357 (49.58%), Query Frame = 0

Query: 11  IFFLFFTISFAELCHPNDKKALLNIKKAFNNPY--ILTSWKPEEDCCTWYCVECDRKSHR 70
           +  L   IS A  C P+D++ALL  + A + PY  I  SW  ++ C  WY + CD  +HR
Sbjct: 7   VLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQDCCHNWYGISCDSLTHR 66

Query: 71  IVSLTIFADTR------------LSGQIPPFVGDLPYLENLMFHKLPNLTGPIQPAIAKL 130
           +  + +  ++             ++G I   + +L  L  +       ++G I   I +L
Sbjct: 67  VADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPKCITRL 126

Query: 131 HNLKYLDLSWNSLTGSIPNFLSSLTNLDLLDLSFNRLTGSIPSSLANLQRLGTLHLDRNK 190
             L+ LDL  N ++G IP  +  L  L +L+++ NR++GSIP SL NL  L  L L  N 
Sbjct: 127 PFLRTLDLIGNQISGGIPYDIGRLNRLAVLNVADNRISGSIPKSLTNLSSLMHLDLRNNL 186

Query: 191 LTGPIPDSFGNFKGKIPYLYLSHNQLSGKIPTSLERV-DFNVIDLSRNRLEG-------- 250
           ++G IP   G  K  +    LS N+++G+IP SL  +     +DLS N+L G        
Sbjct: 187 ISGVIPSDVGRLK-MLSRALLSGNRITGRIPESLTNIYRLADVDLSGNQLYGTIPPSLGR 246

Query: 251 ---------DGSVIFGGKKTTEI------VDLSRNLLEFDMSKVVLPRS-LTWLDLNHNK 310
                    DG+ I G    T +      ++LSRNLL+  + +   PRS  T LDL++N 
Sbjct: 247 MSVLATLNLDGNKISGEIPQTLMTSSVMNLNLSRNLLQGKIPEGFGPRSYFTVLDLSYNN 306

Query: 311 IFGEIPVMAVNLQ-LQLLNVSYNKLCGRIPVGGKLQSFDVYSYSNNKCLCGKPLGKC 328
           + G IP        +  L++S+N LCGRIPVG      +  S+  N CLCGKPL  C
Sbjct: 307 LKGPIPRSISGASFIGHLDLSHNHLCGRIPVGSPFDHLEAASFMFNDCLCGKPLRAC 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050911.2e-12467.48Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW814.7e-11665.16Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J99.1e-11261.18Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J83.5e-11160.12Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9LH526.8e-8350.00Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1ELI84.1e-192100.00polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111435518 P... [more]
A0A6J1HW964.3e-18998.17polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111467229 PE=... [more]
Q7X9Q73.1e-16384.69Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=... [more]
A0A5D3BX473.1e-16384.69Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
K9MZG26.9e-16384.69Polygalacturonase-inhibiting protein OS=Cucumis melo subsp. agrestis OX=217619 G... [more]
Match NameE-valueIdentityDescription
XP_022928674.18.6e-192100.00polygalacturonase inhibitor-like [Cucurbita moschata] >XP_023543570.1 polygalact... [more]
KAG6603233.14.2e-19199.39Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022967838.18.9e-18998.17polygalacturonase inhibitor-like [Cucurbita maxima][more]
NP_001303611.16.4e-16384.69polygalacturonase inhibitor-like precursor [Cucumis melo] >AAP41199.1 polygalact... [more]
AFY10521.11.4e-16284.69polygalacturonase-inhibiting protein [Cucumis melo subsp. agrestis][more]
Match NameE-valueIdentityDescription
AT5G06860.16.5e-11361.18polygalacturonase inhibiting protein 1 [more]
AT5G06870.12.5e-11260.12polygalacturonase inhibiting protein 2 [more]
AT3G12145.14.8e-8450.00Leucine-rich repeat (LRR) family protein [more]
AT3G12610.17.3e-4832.97Leucine-rich repeat (LRR) family protein [more]
AT3G20820.13.4e-4533.05Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00019LEURICHRPTcoord: 140..153
score: 54.82
coord: 119..132
score: 55.09
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 3..327
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 3..327
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 23..326
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 142..202
e-value: 3.6E-7
score: 29.9
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 118..140
e-value: 0.0012
score: 19.1
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 118..140
score: 7.673451
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 22..328
e-value: 2.3E-86
score: 291.8
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 26..63
e-value: 2.4E-11
score: 43.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh03G000790.1CmoCh03G000790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0090353 polygalacturonase inhibitor activity
molecular_function GO:0005515 protein binding