CmoCh03G000420 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh03G000420
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Ef-hand calcium binding protein
Location	Cmo_Chr03: 758943 .. 762378 (-)
RNA-Seq Expression	CmoCh03G000420
Synteny	CmoCh03G000420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAGCCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGTAATCTCTCTGCTCCCTCTATCTCCTTTTTGTTTGCCCTGGCAACAATAGAATTTCATGTGGCTATGAAAACAAGGAAGAGAGAACCTGTAGGAATCTGGAGGGCCCAGATAGAGAAAAAAGACAAACAGTTAGGCCAGTGGGGCAGGGGAGGCCCTTATCGGGGAATCTACTATTAGTTTAACCTAATTTGAACATAATCTGATAGAGATCAATCTCTGTTTATAAATTGAACGCTTCATTGTCAAAACTTTCGAGGTGAATTTCATATAATTGCATAGAATTGTCATTTTCCTTCTTAGTGATTTGATTGTTTCTTATTATGGTTAGTTGATCTGTTCATGTTAGGAATTACGACTTTCTATAATGATACGATATTGTCCACTTTGAGCACAAACTCTCAACTTTACTTTTGGTTTCCTCAAAAGGCCTTAGAGTGTTCTTACTTATAAACCCATGATCTTCCTCCTAATTAGCCAATGTGAGACTACTCCTGTCAATAATTCTCAACAATCCTCTCCTCGAACAAAGCACATCACAGAGCCTCCCCTGAGGCCTATGGAGCCCTCAGATAGTCTCCCCTTAATCGAAGCTCGACTCCTTTCTTTGGAACCCCTTTTGTTCGACACTTGAGTCATTTTTTACTATACCTTCGAGACTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGAGCATGACTCTGACACTCCACAATGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGACCTTGCTTTTGGTTTCTCAAAAGGCCTCATACCAATTGAGATATTGTTCCTTACTTGTAAACCCACGATATTCCTCTTAATTAGCCAATGTAGGACTACTCCTTTCAATAATTCTCAACCGCTCCTAAGGTTCATAAATCATACCTATGAACATCATGAACACATTCTTTCTGTTACAAGGTAATCACTCCTCCATTTGAATTGGAGTGATGGTTAAATTGTCTGTTCTTAATCTGCTCGAATCATCTTATTATCATTATCATTAATTTTTTATGCATTTGGCCTTGAGTGACCCTCATCTGGAAATCCTGCTTTTTATCATTCTATTGATGTTGTCTTTTGCATTTCGTTTCTGAAAAACAGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGTAAGCTGAAGATTTGATATCATTTCAGCCAACATTGTCTAACTGACTAACTATTTATTTGATCTTTTTCAGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTAGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTACGTTTGTCTTCCTTACTTTTTCTTTTTATAACTGGGAGTTGAAGCTTTGTTCCCACGTACCGGGAGGTCACCCACACCCGCCCCAAGGCCCGGACCCGGGAGACATCAAGGGTCTTTGGTATTAGTTTGTCTTCCTTACTCTTGATTTTACGTGGTACTCCTGATGAGAGTTTCTGAGTTACCCTTTAGTGTTGAGTTTTCACTTCACATTAATCACTGTTCTTATCAATTCTTTTTATTTTTGCAATTGCAGGTGTTGTCTTACAGTCAAGGTGAGCTTTTTTTATTCTATACTCTTTAAATGTGATGACATGATCTTTGAATCTGTGTATTTAGTCATGCATATTTGCTTAGTTGTCTTGATTTTGTTCCACTCATGTTGATGCCTATGAATACCCCGAATACTTTTGTAACAGCCCAAGTCCACCGCTAGCCGATATTGTCTGTTTCGGCCCGTTACATATTGCCGTCAACCTCATGATTTTAAAATGCGTCTACTAGGAAGAGGTTTTCACACCCTTATAAAGAATGCTTTGTTCCCCTCTCCAACATATGTGATATCTCACAATTCACTCTCTTTTGGGACCAGCATCCTCGTTGACACTCGTTCCCATCTCCAATCGATGTGGGATCTCACAATTCACCCCCCTTCGAGACTCAGTATCCTTGCTAGCACGCTGCTCGATGCCTAGCTTTGATACCATTTGTAGCAGCCTAAACCCACCACTAGCAATATCCGTTCGGCCCATGACGTATCGCCATTAGCCCCACGGTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCTACACCCTTATAAACAATGTTTCGTTCCCCTCTCCAACCAATGTGGTATCTCATAATCCACCTCCCTTCGAGGCCAGCATCCTCGCTAACACTCGTTCCACTCTCCAATCAACGTAGGATCTCACAATCAATTACACCATTTGAACACCCATGGCTATCAAGTAGTTAATTACCATATAATTTCACTACGAGAAATTACTACTATCGTTTTTCGTTTTTCTCCTTTCATGTAACATGCAATGGTCTTTCACACACCGTTATTTTGAACTCCTCGAATGAACCGTTAACCCGATCCTTCCAAAACAAAGCCATTCGATCTTTGGGACTAAAACTCGAAGTGACCGTGATGTAAAAATTTACAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGAGGAGTTCTTCAGCCTTCGTTCCATCAGGTACGTTCATTTGGCTTTTCCTTCTGATTTCCATTGTGTTTGATAGAATATATTTATGCAGCCTTATCCTTATCCTATCCCATATTTCCAATTTCTCGTTTGACTGTAAAATTTAGGTCATTGTGTCATATATGATGTAATGTGCAACGATTCATTGGGCTAATGGTATTATTGAATACTCTTGCCCCTATGG

mRNA sequence

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAGCCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTAGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGAGGAGTTCTTCAGCCTTCGTTCCATCAGGTACGTTCATTTGGCTTTTCCTTCTGATTTCCATTGTGTTTGATAGAATATATTTATGCAGCCTTATCCTTATCCTATCCCATATTTCCAATTTCTCGTTTGACTGTAAAATTTAGGTCATTGTGTCATATATGATGTAATGTGCAACGATTCATTGGGCTAATGGTATTATTGAATACTCTTGCCCCTATGG

Coding sequence (CDS)

ATGTCAGGTTACCGAAATCAACCTTCCGGTTACGGTTATGGTTACGGCGGTGGCGGTGGTGGTGGTGGTTACGGAGCTCCACCGCCGACGTCTCAGCCGTACAGTTCTCCACCCTACGGCTCGCAACATCCTCCGCCACAGTCACAGCAGCCGTACGCTCCGGTGGCCGCGCAATATGGCGCACCGTCGGCACCGTATGGATCATCTCCGGGCGAAAAGCCGCCGAAGGACAAGCCTAATCAGTCTTACGGCGGATCAGCCAGCGGTGGTCATGGTTATCCACCGCCTTCTGCGTATGGAAGCCCATTCGCCTCACTCATGCCCTCCGCCTTTCCGCCTGGGACTGATCCGAGCATCGTTGCGTGTTTTCAAGTAGCCGATCGGGATAGGAGTGGGTTTATTGATGATAAGGAGTTGCAAGGTGTTCTGTCATCGTATAATCAGAAGTTTAGCATGAGAACTGTTCACCTCCTCATGTTTCATTTCACCAACACAAGCACCAGGAAGATCGGACCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCTAAGCTGGAGGGGTATTTTTGAGAGATTTGATTCAGACCGAAGTGGAAAAATTGATTCCAGAGAGTTGAAAGAAGCACTTTTCAGTTTGGGCTTTTCTGTCTCACCAATGATCTTAGACTTGCTGGTGTCTAAGTTTGACAAAAGTGGTGGGAATAGCAAGGCCATTGAGTATGATAATTTTATAGAGTGTTGTCTTACAGTCAAGGGGCTGACCGAAAAATTCAAGGAAAAGGACACCATGTACTCTGGTTCAGCAACCTTCAGTTACGAGGCTTTCATGTTGACAGTCCTACCATTTCTCATTGCCTGA

Protein sequence

MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA

Homology

[Close]

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.7e-93
Identity = 205/345 (59.42%), Postives = 230/345 (66.67%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQPY 60
           MSGY   PS  GYGYGG         PPP   PY S     PPY   GS  PPP      
Sbjct: 1   MSGY--PPSSQGYGYGGN--------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSAS 60

Query: 61  APVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS-------------------- 120
           +P A  YGA        PSAPY S PG+  KP K+KP+ +                    
Sbjct: 61  SPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSD 120

Query: 121 ----------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPSIV 180
                           YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+IV
Sbjct: 121 YGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIV 180

Query: 181 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 240
           ACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+
Sbjct: 181 ACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFF 240

Query: 241 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 286
            L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEY
Sbjct: 241 SLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEY 300

[Close]

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.2e-93
Identity = 209/360 (58.06%), Postives = 233/360 (64.72%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS----PPYGSQ-----------HPP 60
           MSGY     GYGYGYGGG        PPP   PYSS    PPYGS               
Sbjct: 1   MSGYPPTSQGYGYGYGGG------NQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASK 60

Query: 61  PQSQQPYAPV--AAQYGA--PSAPYGSSPGE--KPPKDKP-------------------- 120
           PQS    AP   ++ YGA  PSAPY  SPG+  KPPK+KP                    
Sbjct: 61  PQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYG 120

Query: 121 -------------------------NQSYGGS----ASGGH-----GYPPPSAYGSPFAS 180
                                      SYGG+    AS GH     GYPP ++YGSPFAS
Sbjct: 121 AGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFAS 180

Query: 181 LMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNT 240
           L+PS F PGTDP+IVACFQ AD+D SGFIDDKELQG LSSY Q+FSMRTVHLLM+ FTN+
Sbjct: 181 LIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS 240

Query: 241 STRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLV 286
           +  KIGPKEF ALFY L +WR IFER D DRSG+ID  EL++AL SLGFSVSP++LDLLV
Sbjct: 241 NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLV 300

[Close]

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 7.0e-92
Identity = 193/296 (65.20%), Postives = 219/296 (73.99%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPP---TSQPYSSPPYGSQHP----PPQSQQPYA 60
           M+GY   P G GY Y   GG GGYGAPPP   +S   S+PPYG++ P       S  PY 
Sbjct: 1   MAGYPPNP-GSGYPY---GGAGGYGAPPPPYGSSPAPSAPPYGAKPPKEGKTSSSSAPYY 60

Query: 61  PVAAQYGAP--SAPYGSSPG-EKPPKDKP-NQSYGGSASGGHGYPPPSAYGSPFASLMPS 120
                YGAP  + PYGS  G   PP  +P    YG        Y  P  YGSPFASL+PS
Sbjct: 61  GGGGGYGAPPSTQPYGSGGGYGAPPSSQPYGAPYGAPPPSSAPYGAPGGYGSPFASLVPS 120

Query: 121 AFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRK 180
           AFPPGTDP++VACFQ ADRD SG IDDKELQ  LS Y+Q FS+RTVHLLM+ FTNT+ RK
Sbjct: 121 AFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVHLLMYLFTNTNVRK 180

Query: 181 IGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFD 240
           IGPKEF ++FY L +WR IFERFD DRSGKID+ EL++AL SLG+SVSP +LDLLVSKFD
Sbjct: 181 IGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSVSPTVLDLLVSKFD 240

Query: 241 KSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           K+GG +KAIEYDNFIECCLTVKGLTEKFKEKDT +SGSATF+YEAFMLTVLPFLIA
Sbjct: 241 KTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFMLTVLPFLIA 292

[Close]

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 9.2e-36
Identity = 92/228 (40.35%), Postives = 138/228 (60.53%), Query Frame = 0


Query: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYP--PPSAYGSPFASLMPSAFPPGTDPS 120
           APSAP       + P+    Q + G +   + YP   P+   S ++ +    F P T P 
Sbjct: 8   APSAP-------ELPESFVQQQHDGESRYTYAYPSYQPTQQFSSYSGM----FSPETHPE 67

Query: 121 IVACFQVADRDRSGFIDDKELQGVLS-SYNQKFSMRTVHLLMFHF--TNTSTRKIGPKEF 180
           IV  F+ ADR+RSGF+++ EL+  LS S     S RT+ LL+F +     S  ++GPKE+
Sbjct: 68  IVRSFESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIYKIPVDSLLRLGPKEY 127

Query: 181 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 240
           + L+  L  WR IF R+D DRSGK++S +L++A ++LG  +   +  L+VS+FD   G +
Sbjct: 128 VELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDGTGKT 187

Query: 241 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFL 284
             + +D+F+EC + VKGLTEKF+E D  Y+G AT SY+ FML V+PF+
Sbjct: 188 VDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSYDVFMLMVIPFI 224

[Close]

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot
Match: Q9UBV8 (Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.2e-27
Identity = 101/277 (36.46%), Postives = 142/277 (51.26%), Query Frame = 0


Query: 9   SGYGYGYG-GGGGGGGYGAPPPT---SQPYSSPPYGSQHPPPQSQQPYAPVAAQYGAPS- 68
           + Y Y  G  G  G   GAPP +     P S   YGS  PP       AP    YG P+ 
Sbjct: 2   ASYPYRQGCPGAAGQAPGAPPGSYYPGPPNSGGQYGSGLPPGGGYGGPAP-GGPYGPPAG 61

Query: 69  -APYGSSPGEKPPKDKPNQSYGGSASGG-HGYPPPSAYGSPFASLM-PSAFPPGTDPSIV 128
             PYG       P   P   YGG+A GG +G PPPS+YG+    L      PP  DP   
Sbjct: 62  GGPYGHPNPGMFPSGTPGGPYGGAAPGGPYGQPPPSSYGAQQPGLYGQGGAPPNVDPEAY 121

Query: 129 ACFQVADRDRSGFIDDKELQGVLSSYN-QKFSMRTVHLLMFHFTNTSTRKIGPKEFIALF 188
           + FQ  D D SG+I  KEL+  L + N   F+  T  +++  F  T + +I    F AL+
Sbjct: 122 SWFQSVDSDHSGYISMKELKQALVNCNWSSFNDETCLMMINMFDKTKSGRIDVYGFSALW 181

Query: 189 YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE 248
             +  W+ +F+++D DRSG I   EL++AL  +G+++SP    LLVS++     N  A++
Sbjct: 182 KFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSAN-PAMQ 241

Query: 249 YDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFM 277
            D FI+ C  ++ LTE F+EKDT   G+   S+E F+
Sbjct: 242 LDRFIQVCTQLQVLTEAFREKDTAVQGNIRLSFEDFV 276

[Close]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL
Match: A0A6J1ES88 (probable calcium-binding protein CML49 OS=Cucurbita moschata OX=3662 GN=LOC111435509 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 1.9e-156
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120
           APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV
Sbjct: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120

Query: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180
           ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY
Sbjct: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180

Query: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240
           GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY
Sbjct: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240

Query: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 285

[Close]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL
Match: A0A6J1HXZ3 (probable calcium-binding protein CML50 OS=Cucurbita maxima OX=3661 GN=LOC111467251 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.2e-152
Identity = 280/285 (98.25%), Postives = 280/285 (98.25%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGYRNQPSGYGY Y   GGGGGYGAPPPTSQPYSSPPYGSQHPP QSQQPYAPVAAQYG
Sbjct: 1   MSGYRNQPSGYGYSY---GGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120
           APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV
Sbjct: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120

Query: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180
           ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY
Sbjct: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180

Query: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240
           GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY
Sbjct: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240

Query: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 282

[Close]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 1.4e-135
Identity = 253/290 (87.24%), Postives = 265/290 (91.38%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGY NQPSGYGYGY   GG GGYGAPPPT+QPY S PYGSQHPPP SQQPYAPVAA YG
Sbjct: 1   MSGYPNQPSGYGYGY---GGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGG-----SASGGHGYPPPSAYGSPFASLMPSAFPPGT 120
           APSAPYGS+PGEKP KDKPNQS+GG      + GGHGYPPPSAYGSPFASL+PSAFPPGT
Sbjct: 61  APSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGT 120

Query: 121 DPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEF 180
           DPSIV CFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TRKIGPKEF
Sbjct: 121 DPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEF 180

Query: 181 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 240
           IALFYGL SWRGIFERFDSDRSGKID  EL+EAL SLGF+VSPM+LDLLVSKFDKSGG S
Sbjct: 181 IALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKFDKSGGKS 240

Query: 241 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           KAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL
Match: A0A6J1EBH1 (probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432514 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 6.9e-135
Identity = 252/290 (86.90%), Postives = 264/290 (91.03%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGY NQPSGYGYGY   GG GGYGAPPPT+QPY S PYGSQHPPP SQQPYAPVAA YG
Sbjct: 1   MSGYPNQPSGYGYGY---GGAGGYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAAPYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGG-----SASGGHGYPPPSAYGSPFASLMPSAFPPGT 120
           APSAPYGS+P EKP KDKPNQS+GG      + GGHGYPPPSAYGSPFASL+PSAFPPGT
Sbjct: 61  APSAPYGSNPSEKPHKDKPNQSHGGVGGSHQSYGGHGYPPPSAYGSPFASLLPSAFPPGT 120

Query: 121 DPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEF 180
           DPSIV CFQVAD+D SGFIDDKELQGVLSSYNQ+FS+RTVHLLM+HFTNT+TRKIGPKEF
Sbjct: 121 DPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQRFSIRTVHLLMYHFTNTNTRKIGPKEF 180

Query: 181 IALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNS 240
           IALFYGL SWRGIFERFDSDRSGKID  EL+EAL SLGFSVSPM+LDLLVSKFDKSGG S
Sbjct: 181 IALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFSVSPMVLDLLVSKFDKSGGKS 240

Query: 241 KAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           KAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 KAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL
Match: A0A6J1DIS2 (probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021303 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 2.5e-132
Identity = 249/297 (83.84%), Postives = 267/297 (89.90%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGG---GGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAA 60
           MSGY NQPSGYGYGYGG   GGG GGYGAPPPT+QPY+S  YG Q PPP SQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 61  QYGAPSAPYGSSPGEKPPKDKPNQSYGGS---------ASGGHGYPPPSAYGSPFASLMP 120
            YGAPSAPYGSS G+KPPKDKP+QSYGG+         A GGH YPPP++YG+PFASL+P
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 121 SAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTR 180
           SAFPPGTDPSIVACFQVAD+D SGFIDDKELQGVLSSYNQKFS+RTVHLLM+HFTNT+TR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 181 KIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKF 240
           KIGPKEFIALFYGL SWRGIFERFDSDRSGKIDS EL+EAL SLGF+VSP++LDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 241 DKSGGNSKAIEYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           DKSGG SKAIEYDNFIECCLTVKGLTEKFKEKDT YSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 297

[Close]

BLAST of CmoCh03G000420 vs. NCBI nr
Match: XP_022928660.1 (probable calcium-binding protein CML49 [Cucurbita moschata])

HSP 1 Score: 561.6 bits (1446), Expect = 3.9e-156
Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120
           APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV
Sbjct: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120

Query: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180
           ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY
Sbjct: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180

Query: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240
           GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY
Sbjct: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240

Query: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 285

[Close]

BLAST of CmoCh03G000420 vs. NCBI nr
Match: XP_023544753.1 (probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.0 bits (1434), Expect = 9.5e-155
Identity = 285/286 (99.65%), Postives = 285/286 (99.65%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGY-GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQY 60
           MSGYRNQPSGYGYGY GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQY
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQY 60

Query: 61  GAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSI 120
           GAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSI
Sbjct: 61  GAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSI 120

Query: 121 VACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALF 180
           VACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALF
Sbjct: 121 VACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALF 180

Query: 181 YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE 240
           YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE
Sbjct: 181 YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE 240

Query: 241 YDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           YDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 YDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286

[Close]

BLAST of CmoCh03G000420 vs. NCBI nr
Match: KAG7033501.1 (putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556.6 bits (1433), Expect = 1.2e-154
Identity = 285/287 (99.30%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGY--GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGY  GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of CmoCh03G000420 vs. NCBI nr
Match: XP_022967874.1 (probable calcium-binding protein CML50 [Cucurbita maxima])

HSP 1 Score: 546.6 bits (1407), Expect = 1.3e-151
Identity = 280/285 (98.25%), Postives = 280/285 (98.25%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQYG 60
           MSGYRNQPSGYGY Y   GGGGGYGAPPPTSQPYSSPPYGSQHPP QSQQPYAPVAAQYG
Sbjct: 1   MSGYRNQPSGYGYSY---GGGGGYGAPPPTSQPYSSPPYGSQHPPSQSQQPYAPVAAQYG 60

Query: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120
           APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV
Sbjct: 61  APSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPSIV 120

Query: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180
           ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY
Sbjct: 121 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 180

Query: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240
           GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY
Sbjct: 181 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 240

Query: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 286
           DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DNFIECCLTVKGLTEKFKEKDTMYSGSATFSYEAFMLTVLPFLIA 282

[Close]

BLAST of CmoCh03G000420 vs. NCBI nr
Match: KAG6603192.1 (putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.4 bits (1339), Expect = 9.8e-144
Identity = 265/267 (99.25%), Postives = 265/267 (99.25%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGY--GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60
           MSGYRNQPSGYGYGY  GGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ
Sbjct: 1   MSGYRNQPSGYGYGYGGGGGGGGGGYGAPPPTSQPYSSPPYGSQHPPPQSQQPYAPVAAQ 60

Query: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120
           YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS
Sbjct: 61  YGAPSAPYGSSPGEKPPKDKPNQSYGGSASGGHGYPPPSAYGSPFASLMPSAFPPGTDPS 120

Query: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180
           IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL
Sbjct: 121 IVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIAL 180

Query: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240
           FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI
Sbjct: 181 FYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAI 240

Query: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 266
           EYDNFIECCLTVKGLTEKFKEKDTMYS
Sbjct: 241 EYDNFIECCLTVKGLTEKFKEKDTMYS 267

[Close]

BLAST of CmoCh03G000420 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 344.0 bits (881), Expect = 1.2e-94
Identity = 205/345 (59.42%), Postives = 230/345 (66.67%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQPY 60
           MSGY   PS  GYGYGG         PPP   PY S     PPY   GS  PPP      
Sbjct: 1   MSGY--PPSSQGYGYGGN--------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSAS 60

Query: 61  APVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS-------------------- 120
           +P A  YGA        PSAPY S PG+  KP K+KP+ +                    
Sbjct: 61  SPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSD 120

Query: 121 ----------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPSIV 180
                           YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+IV
Sbjct: 121 YGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIV 180

Query: 181 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 240
           ACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+
Sbjct: 181 ACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFF 240

Query: 241 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 286
            L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEY
Sbjct: 241 SLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEY 300

[Close]

BLAST of CmoCh03G000420 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.5e-94
Identity = 209/360 (58.06%), Postives = 233/360 (64.72%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS----PPYGSQ-----------HPP 60
           MSGY     GYGYGYGGG        PPP   PYSS    PPYGS               
Sbjct: 1   MSGYPPTSQGYGYGYGGG------NQPPPPQPPYSSGGNNPPYGSSTTSSPYAVPYGASK 60

Query: 61  PQSQQPYAPV--AAQYGA--PSAPYGSSPGE--KPPKDKP-------------------- 120
           PQS    AP   ++ YGA  PSAPY  SPG+  KPPK+KP                    
Sbjct: 61  PQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSSDYGSYG 120

Query: 121 -------------------------NQSYGGS----ASGGH-----GYPPPSAYGSPFAS 180
                                      SYGG+    AS GH     GYPP ++YGSPFAS
Sbjct: 121 AGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASYGSPFAS 180

Query: 181 LMPSAFPPGTDPSIVACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNT 240
           L+PS F PGTDP+IVACFQ AD+D SGFIDDKELQG LSSY Q+FSMRTVHLLM+ FTN+
Sbjct: 181 LIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLMYLFTNS 240

Query: 241 STRKIGPKEFIALFYGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLV 286
           +  KIGPKEF ALFY L +WR IFER D DRSG+ID  EL++AL SLGFSVSP++LDLLV
Sbjct: 241 NAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLV 300

[Close]

BLAST of CmoCh03G000420 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.8e-74
Identity = 171/305 (56.07%), Postives = 194/305 (63.61%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQPY 60
           MSGY   PS  GYGYGG         PPP   PY S     PPY   GS  PPP      
Sbjct: 1   MSGY--PPSSQGYGYGGN--------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSAS 60

Query: 61  APVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS-------------------- 120
           +P A  YGA        PSAPY S PG+  KP K+KP+ +                    
Sbjct: 61  SPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSD 120

Query: 121 ----------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPSIV 180
                           YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+IV
Sbjct: 121 YGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIV 180

Query: 181 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 240
           ACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+
Sbjct: 181 ACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFF 240

Query: 241 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 246
            L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEY
Sbjct: 241 SLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEY 295

[Close]

BLAST of CmoCh03G000420 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.8e-74
Identity = 171/305 (56.07%), Postives = 194/305 (63.61%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQPY 60
           MSGY   PS  GYGYGG         PPP   PY S     PPY   GS  PPP      
Sbjct: 1   MSGY--PPSSQGYGYGGN--------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSAS 60

Query: 61  APVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS-------------------- 120
           +P A  YGA        PSAPY S PG+  KP K+KP+ +                    
Sbjct: 61  SPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSD 120

Query: 121 ----------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPSIV 180
                           YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+IV
Sbjct: 121 YGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIV 180

Query: 181 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIGPKEFIALFY 240
           ACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIGPKEF +LF+
Sbjct: 181 ACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFF 240

Query: 241 GLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIEY 246
            L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIEY
Sbjct: 241 SLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEY 295

[Close]

BLAST of CmoCh03G000420 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 272.3 bits (695), Expect = 4.4e-73
Identity = 171/306 (55.88%), Postives = 194/306 (63.40%), Query Frame = 0


Query: 1   MSGYRNQPSGYGYGYGGGGGGGGYGAPPPTSQPYSS-----PPY---GSQHPPPQSQQPY 60
           MSGY   PS  GYGYGG         PPP   PY S     PPY   GS  PPP      
Sbjct: 1   MSGY--PPSSQGYGYGGN--------PPPPQPPYGSTGNNPPPYGSSGSNPPPPYGSSAS 60

Query: 61  APVAAQYGA--------PSAPYGSSPGE--KPPKDKPNQS-------------------- 120
           +P A  YGA        PSAPY S PG+  KP K+KP+ +                    
Sbjct: 61  SPYAVPYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSD 120

Query: 121 ----------------YGGSA-----SGGHGYPPPSA-YGSPFASLMPSAFPPGTDPSIV 180
                           YGG+       GG+G PPP A YGSPFASL+PSAFPPGTDP+IV
Sbjct: 121 YGGYGGAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIV 180

Query: 181 ACFQVADRDRSGFIDDKELQGVLSSYNQKFSMRTVHLLMFHFTNTSTRKIG-PKEFIALF 240
           ACFQ ADRD SGFIDDKELQG LSSYNQ FS+RTVHLLM+ FTN++ RKIG PKEF +LF
Sbjct: 181 ACFQAADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLF 240

Query: 241 YGLLSWRGIFERFDSDRSGKIDSRELKEALFSLGFSVSPMILDLLVSKFDKSGGNSKAIE 246
           + L +WR IFERFD DRSG+ID+ EL++AL SLGFSVSP+ILDLLVSKFDKSGG ++AIE
Sbjct: 241 FSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIE 296

The following BLAST results are available for this feature:

BLAST of CmoCh03G000420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W4L0	1.7e-93	59.42	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ...	[more]
Q9FYE4	2.2e-93	58.06	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ...	[more]
Q2QY10	7.0e-92	65.20	Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2...	[more]
Q9ZQH1	9.2e-36	40.35	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ...	[more]
Q9UBV8	1.2e-27	36.46	Peflin OS=Homo sapiens OX=9606 GN=PEF1 PE=1 SV=1	[more]

BLAST of CmoCh03G000420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ES88	1.9e-156	100.00	probable calcium-binding protein CML49 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HXZ3	6.2e-152	98.25	probable calcium-binding protein CML50 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A6J1HP98	1.4e-135	87.24	probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660...	[more]
A0A6J1EBH1	6.9e-135	86.90	probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1DIS2	2.5e-132	83.84	probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673...	[more]

BLAST of CmoCh03G000420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022928660.1	3.9e-156	100.00	probable calcium-binding protein CML49 [Cucurbita moschata]	[more]
XP_023544753.1	9.5e-155	99.65	probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo]	[more]
KAG7033501.1	1.2e-154	99.30	putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022967874.1	1.3e-151	98.25	probable calcium-binding protein CML50 [Cucurbita maxima]	[more]
KAG6603192.1	9.8e-144	99.25	putative calcium-binding protein CML49, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of CmoCh03G000420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10300.3	1.2e-94	59.42	Calcium-binding EF-hand family protein	[more]
AT5G04170.1	1.5e-94	58.06	Calcium-binding EF-hand family protein	[more]
AT3G10300.2	1.8e-74	56.07	Calcium-binding EF-hand family protein	[more]
AT3G10300.4	1.8e-74	56.07	Calcium-binding EF-hand family protein	[more]
AT3G10300.5	4.4e-73	55.88	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 118..146 e-value: 0.19 score: 20.8 coord: 184..212 e-value: 0.012 score: 24.8
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 185..213 e-value: 2.2E-5 score: 23.9
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 119..142 e-value: 8.0E-5 score: 22.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 114..149 score: 10.384886
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 180..215 score: 11.75176
None	No IPR available	GENE3D	1.10.238.10		coord: 101..284 e-value: 5.2E-37 score: 129.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..51
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..95
None	No IPR available	PANTHER	PTHR46824:SF1	CALCIUM-BINDING PROTEIN CML49-RELATED	coord: 1..285
None	No IPR available	CDD	cd16180	EFh_PEF_Group_I	coord: 118..282 e-value: 2.05633E-73 score: 220.478
IPR044590	Probable calcium-binding protein CML48/49/50	PANTHER	PTHR46824	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 1..285
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 193..205
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 112..280

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh03G000420.1	CmoCh03G000420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding