CmoCh02G018500 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G018500
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGnk2-homologous domain-containing protein
LocationCmo_Chr02: 10458942 .. 10459346 (+)
RNA-Seq ExpressionCmoCh02G018500
SyntenyCmoCh02G018500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGGTTGTAGCCTCAAGATTTCAGCCGCTTTTCTGACTTTGTTTGGCTTCTTCGCTATGGTGGTGAGCGTTCCAAACACGGGAGTCAAGTCAGTTTTGTGCAACTCAGGAATTTTCACGGGGGGCGATCCTTTTGCCGTGAGTCTGGATTACGTTCTAAAAGAATTGGAGAGTTCGACTCCCACTACAAACAATTATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTACGGCCATGCTTCCTGCAACCAAAGCCTTACAACCTCAGACTGCACCACTTGTCTTGGCGCTGCTAAGACCAACATGTTGGGGTCGTGTCAAATGAGGATTGGTGCTCGTGCGGTGCTCAATGACTGTTCAATTAGGTACGAGCAAAACCCATTTGATGATTAG

mRNA sequence

ATGGTTGGTTGTAGCCTCAAGATTTCAGCCGCTTTTCTGACTTTGTTTGGCTTCTTCGCTATGGTGGTGAGCGTTCCAAACACGGGAGTCAAGTCAGTTTTGTGCAACTCAGGAATTTTCACGGGGGGCGATCCTTTTGCCGTGAGTCTGGATTACGTTCTAAAAGAATTGGAGAGTTCGACTCCCACTACAAACAATTATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTACGGCCATGCTTCCTGCAACCAAAGCCTTACAACCTCAGACTGCACCACTTGTCTTGGCGCTGCTAAGACCAACATGTTGGGGTCGTGTCAAATGAGGATTGGTGCTCGTGCGGTGCTCAATGACTGTTCAATTAGGTACGAGCAAAACCCATTTGATGATTAG

Coding sequence (CDS)

ATGGTTGGTTGTAGCCTCAAGATTTCAGCCGCTTTTCTGACTTTGTTTGGCTTCTTCGCTATGGTGGTGAGCGTTCCAAACACGGGAGTCAAGTCAGTTTTGTGCAACTCAGGAATTTTCACGGGGGGCGATCCTTTTGCCGTGAGTCTGGATTACGTTCTAAAAGAATTGGAGAGTTCGACTCCCACTACAAACAATTATGATTTCTACAACATATCTCCTTATCCTAATTCCTTCGCTTACGGCCATGCTTCCTGCAACCAAAGCCTTACAACCTCAGACTGCACCACTTGTCTTGGCGCTGCTAAGACCAACATGTTGGGGTCGTGTCAAATGAGGATTGGTGCTCGTGCGGTGCTCAATGACTGTTCAATTAGGTACGAGCAAAACCCATTTGATGATTAG

Protein sequence

MVGCSLKISAAFLTLFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD
Homology
BLAST of CmoCh02G018500 vs. ExPASy Swiss-Prot
Match: D9IWE0 (Antifungal protein ginkbilobin-like protein OS=Pinus pinaster OX=71647 GN=EAP PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.0e-12
Identity = 45/110 (40.91%), Postives = 55/110 (50.00%), Query Frame = 0

Query: 23  VSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFAYG 82
           V  P T + S  CN      G  F  +L  +L +LE +T  ++ YD Y  S   +  AYG
Sbjct: 22  VGTPRTNLVSSACNGQKIQSGSSFYSTLGSLLVDLEGNT-ASSGYD-YKASRAGSPTAYG 81

Query: 83  HASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPF 133
            A CNQ L+ SDCT CL      +   C   IGAR  L DC IRYEQ  F
Sbjct: 82  RAVCNQGLSQSDCTACLKNLGGRIWNICGYAIGARVQLTDCFIRYEQYSF 129

BLAST of CmoCh02G018500 vs. ExPASy Swiss-Prot
Match: A4ZDL6 (Antifungal protein ginkbilobin-2 OS=Ginkgo biloba OX=3311 GN=GNK2 PE=1 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-11
Identity = 44/133 (33.08%), Postives = 65/133 (48.87%), Query Frame = 0

Query: 5   SLKISAAFLTLFGFFA---MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESST 64
           ++++++AF+  F   A   ++    NT   S  CN+     G PF  +L  +L +L  +T
Sbjct: 3   TMRMNSAFILAFALAAAMLILTEAANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNT 62

Query: 65  PTTNNYDFYNISPYPNS--FAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAV 124
              + YD+            AYG A+C QS++ SDCT CL      +   C   IGAR  
Sbjct: 63  -AFSGYDYKTSRAGSGGAPTAYGRATCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQ 122

Query: 125 LNDCSIRYEQNPF 133
           L DC I+YEQ  F
Sbjct: 123 LVDCFIQYEQRSF 134

BLAST of CmoCh02G018500 vs. ExPASy Swiss-Prot
Match: A9NQT1 (Antifungal protein ginkbilobin-like protein 1 OS=Picea sitchensis OX=3332 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-11
Identity = 45/126 (35.71%), Postives = 58/126 (46.03%), Query Frame = 0

Query: 5   SLKISAAFLTLFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTT 64
           S   + A + L    A     PNT   S  CN+     G+PF  +L  +L  LE +T  +
Sbjct: 5   SFGFALAVMVLAVLVASAAGAPNTNFVSSACNTKKIPSGNPFFNNLGALLVNLEKNTAFS 64

Query: 65  N-NYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDC 124
             +Y            AYG   C QS++ SDCT CL      + G C+  IGAR  L DC
Sbjct: 65  GYDYKASRAGSGGAPTAYGRGVCKQSISQSDCTACLTNLGGRIWGICKNAIGARVQLTDC 124

Query: 125 SIRYEQ 130
            IRYEQ
Sbjct: 125 FIRYEQ 130

BLAST of CmoCh02G018500 vs. ExPASy Swiss-Prot
Match: Q3I3X0 (Antifungal protein ginkbilobin-like protein (Fragment) OS=Picea abies OX=3329 GN=EMB24 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.8e-11
Identity = 41/109 (37.61%), Postives = 55/109 (50.46%), Query Frame = 0

Query: 26  PNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNS--FAYGH 85
           PNT   S  CN+     G+PF  +L  +L +L+ +T   + YD+            AYG 
Sbjct: 2   PNTNFVSSACNTQKIPSGNPFFNNLGAMLADLKQNT-AFSGYDYKTSRAGSGGAPTAYGR 61

Query: 86  ASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPF 133
           A C  S++ SDCT CL      + G C   IGAR  L DC I+YEQ+ F
Sbjct: 62  AICKSSISQSDCTACLSNLVGRIWGICSNAIGARVQLTDCFIQYEQHSF 109

BLAST of CmoCh02G018500 vs. ExPASy Swiss-Prot
Match: Q40849 (Antifungal protein ginkbilobin-like protein 1 OS=Picea glauca OX=3330 GN=EMB24 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 6.3e-11
Identity = 43/129 (33.33%), Postives = 62/129 (48.06%), Query Frame = 0

Query: 5   SLKISAAFLTLFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTT 64
           SL +  A + + G      + PNT   S  CN+     G+PF  +L  +L +L+ +T  +
Sbjct: 14  SLLLLVALMVVMGMDG-AAAAPNTNFVSSACNTQKIPSGNPFFTNLRAMLADLKQNTAFS 73

Query: 65  N-NYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDC 124
             +Y            AYG A C  S++ SDC+ CL      + G C   IGAR  L DC
Sbjct: 74  GFDYKTSRAGSGGAPTAYGRAICKSSISQSDCSACLSNLVGRIWGICSNAIGARVQLTDC 133

Query: 125 SIRYEQNPF 133
            I+YEQ+ F
Sbjct: 134 FIQYEQHSF 141

BLAST of CmoCh02G018500 vs. ExPASy TrEMBL
Match: A0A6J1ESI5 (antifungal protein ginkbilobin-2-like OS=Cucurbita moschata OX=3662 GN=LOC111437337 PE=4 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.9e-59
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0

Query: 21  MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA 80
           MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA
Sbjct: 1   MVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTTNNYDFYNISPYPNSFA 60

Query: 81  YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD 135
           YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD
Sbjct: 61  YGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCSIRYEQNPFDD 114

BLAST of CmoCh02G018500 vs. ExPASy TrEMBL
Match: A0A0A0KGA2 (Gnk2-homologous domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G409950 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.3e-56
Identity = 108/134 (80.60%), Postives = 118/134 (88.06%), Query Frame = 0

Query: 1   MVGCSLKISAAFLTLFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESS 60
           M+G SLKIS AFL  F    MVVS+PNTGV+SVLCNSG FTGGDPF VSLDYVLKEL+S+
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPTTNNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVL 120
           TPT  NYDFYNISPYPN+FAYGHASCNQ+L+TSDCTTCL AAK NMLGSCQMRIG R+VL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 NDCSIRYEQNPFDD 135
           NDC+IRYEQ PFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of CmoCh02G018500 vs. ExPASy TrEMBL
Match: A0A5D3CNH9 (Antifungal protein ginkbilobin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold496G00580 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.9e-55
Identity = 107/134 (79.85%), Postives = 118/134 (88.06%), Query Frame = 0

Query: 1   MVGCSLKISAAFLTLFGFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESS 60
           M+G SLKIS AFL  F    MVVS+PNTGV+SVLCNSG FTGGDPFAVSLDYVLKEL+S+
Sbjct: 1   MIGSSLKISVAFLAFFAVSIMVVSIPNTGVRSVLCNSGTFTGGDPFAVSLDYVLKELQSA 60

Query: 61  TPTTNNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVL 120
           TPT  NYDFYNISPYPN+FAYGHASCNQ+LTTSDCT+CL AA+ NMLGSCQ RIG R+VL
Sbjct: 61  TPTIKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTSCLEAAENNMLGSCQKRIGGRSVL 120

Query: 121 NDCSIRYEQNPFDD 135
           NDC+IRYEQ PFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of CmoCh02G018500 vs. ExPASy TrEMBL
Match: A0A5E4EE49 (PREDICTED: antifungal OS=Prunus dulcis OX=3755 GN=ALMOND_2B017312 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.1e-45
Identity = 90/130 (69.23%), Postives = 107/130 (82.31%), Query Frame = 0

Query: 6   LKISAAFLTLF-GFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTT 65
           +KI+A  +  F GFF +  S P+T + SVLCNSG++T GDPFAVSL YVLKELE++T T 
Sbjct: 6   MKIAATLVVFFIGFFGVATSTPDTNITSVLCNSGLYTAGDPFAVSLAYVLKELETATSTH 65

Query: 66  NNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCS 125
            NYDFY+ISPYPN+FAYGHASCNQ++TT DCTTCLGAAKT+MLG+CQ  IG RAVL DC+
Sbjct: 66  KNYDFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCA 125

Query: 126 IRYEQNPFDD 135
           IRYEQ PF D
Sbjct: 126 IRYEQYPFTD 135

BLAST of CmoCh02G018500 vs. ExPASy TrEMBL
Match: A0A251N2S2 (Gnk2-homologous domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_8G242500 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 9.1e-45
Identity = 89/128 (69.53%), Postives = 107/128 (83.59%), Query Frame = 0

Query: 6   LKISAAFLTLF-GFFAMVVSVPNTGVKSVLCNSGIFTGGDPFAVSLDYVLKELESSTPTT 65
           +KI+A  + +F GFF +  S P+T + SVLCNSG++T GDPFAVSL YVLKELE++T T 
Sbjct: 6   MKIAATIVVVFIGFFGVATSTPDTNITSVLCNSGVYTAGDPFAVSLAYVLKELETATSTH 65

Query: 66  NNYDFYNISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDCS 125
            NYDFY+ISPYPN+FAYGHASCNQ++TT DCTTCLGAAKT+MLG+CQ  IG RAVL DC+
Sbjct: 66  KNYDFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCA 125

Query: 126 IRYEQNPF 133
           IRYEQ PF
Sbjct: 126 IRYEQYPF 133

BLAST of CmoCh02G018500 vs. TAIR 10
Match: AT3G60720.1 (plasmodesmata-located protein 8 )

HSP 1 Score: 41.2 bits (95), Expect = 7.7e-04
Identity = 35/129 (27.13%), Postives = 53/129 (41.09%), Query Frame = 0

Query: 12  FLTLFGFFAMVVSVPNTGVKSVL----CNSGIFTGGDPFAVSLDYVLKEL--ESSTPTTN 71
           F  LF FF    S  ++    +     C+   +T   PF  + D  L  +   SS  + N
Sbjct: 7   FSLLFLFFYSSSSSRSSSESHIFIYGGCSPEKYTPNTPFESNRDTFLSSVVTSSSDASFN 66

Query: 72  NYDFYN--ISPYPNSFAYGHASCNQSLTTSDCTTCLGAAKTNMLGSCQMRIGARAVLNDC 131
           ++   N   S   +S  +G   C   L +SDC+ C+  +   +   C    GA   L  C
Sbjct: 67  SFAVGNDSSSSSSSSAVFGLYQCRDDLRSSDCSKCIQTSVDQITLICPYSYGASLQLEGC 126

Query: 132 SIRYEQNPF 133
            +RYE N F
Sbjct: 127 FLRYETNDF 135

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D9IWE02.0e-1240.91Antifungal protein ginkbilobin-like protein OS=Pinus pinaster OX=71647 GN=EAP PE... [more]
A4ZDL62.8e-1133.08Antifungal protein ginkbilobin-2 OS=Ginkgo biloba OX=3311 GN=GNK2 PE=1 SV=1[more]
A9NQT12.8e-1135.71Antifungal protein ginkbilobin-like protein 1 OS=Picea sitchensis OX=3332 PE=2 S... [more]
Q3I3X02.8e-1137.61Antifungal protein ginkbilobin-like protein (Fragment) OS=Picea abies OX=3329 GN... [more]
Q408496.3e-1133.33Antifungal protein ginkbilobin-like protein 1 OS=Picea glauca OX=3330 GN=EMB24 P... [more]
Match NameE-valueIdentityDescription
A0A6J1ESI52.9e-59100.00antifungal protein ginkbilobin-2-like OS=Cucurbita moschata OX=3662 GN=LOC111437... [more]
A0A0A0KGA22.3e-5680.60Gnk2-homologous domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G40... [more]
A0A5D3CNH91.9e-5579.85Antifungal protein ginkbilobin-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A5E4EE494.1e-4569.23PREDICTED: antifungal OS=Prunus dulcis OX=3755 GN=ALMOND_2B017312 PE=4 SV=1[more]
A0A251N2S29.1e-4569.53Gnk2-homologous domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_8G2... [more]
Match NameE-valueIdentityDescription
AT3G60720.17.7e-0427.13plasmodesmata-located protein 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 38..129
e-value: 5.3E-13
score: 49.4
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 28..132
score: 15.701318
IPR038408Gnk2-homologous domain superfamilyGENE3D3.30.430.20coord: 27..132
e-value: 2.2E-16
score: 61.8
NoneNo IPR availablePANTHERPTHR32080:SF54ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKEcoord: 6..133
NoneNo IPR availablePANTHERPTHR32080ANTIFUNGAL PROTEIN GINKBILOBIN-2-LIKEcoord: 6..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G018500.1CmoCh02G018500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009506 plasmodesma