CmoCh02G015630 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G015630
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptiongeraniol 8-hydroxylase-like
LocationCmo_Chr02: 9037961 .. 9039187 (+)
RNA-Seq ExpressionCmoCh02G015630
SyntenyCmoCh02G015630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTCTTATCTTCGGTGTCTTAATCTGCCTTATTTCCACTTTCATAGCCCTCCGTCTGATTCCGGCGAGAAGAAACCCCAACCTACCTCCCGGACCACGACCTCTTCCGATCATTGGAAATCTCTTGGATCTTGGAGACAATCCCCACCAATCTCTAGCCAAACTGTCCGAGTCCTATGGCCCAATCATGTCGTTGAAACTCGGCCAACTCACCGCCGTCGTCGTATCTTCCCCGGAGACGATTCAGCAAGTCCTTCAAACCCACGACCATTTCCTATCTTTCAGAGCCGTTCCGGATGCTCTATCGCCTTACGACCACGGTCAACTAGCTTTCCCATGGATTCCCGTTTCCCCCACCTACAAAAACATTCGAAAAATCTGCAACACCCATCTTTTATCCCCCAAGACTCTCGACGCCAATCAAAACCTCCGCCGTACCAGGATAGACGACCTTCTTGCTAACATCCGACGAAGCGCTTTGAACGGGGAGGCGGTGGATATTGGGGAAGCTGTGTTTACGACGACCCTTAATTTGATTTCTTATTCGATTTGGTCGGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAATTCAAGGTCACCATGAGAGGACTGATGGAAGAAGCTGGAAAACCAAATATTAGTGACTTTTTCCCTGTGCTGAAGAGGTTGGACATGCAGGGTATAAGGAGGCGCATCACCGTTCATTTTGGAAAGATGTTTGAGATGATCGATGGGATGATTGATGAACGGTTGAAGATGCAGGAATTGCCCGATTTCAGTCCTAAAAATGATATGCTACATCATCTTCTCAACATGAGGGAGGACAACAACGAGATCCCTCTTGATAGAAACCAAATCAAACATTCAATATTGGTGAGGAGCACTCAATACTCTGTTTCTATTTCATTGGATGCATAAATAACATTCGAGTTGGAATTGGAAATGGTTGTAGGTGTTATTTACCGGAGGAACAGATACGACTACATCAATCGTGCAATGGGCAATGACACATCTTTTGAAGAACCCAGAAACCATGTTGAAACTCAAAGAAGAACTCTTGCGAGTGATTGGGAAAGGAAATCCAGTTGAAGAATCGCACATCAACTGTTGGAAAGAGGGATAGTGAAAGAGATAGGAATAGATGAGAATAGATAGAAGCTGCAGTGCTTTATTAATGTCTACTGACTTGATGCTTACAGGGTTACACAATTCCAG

mRNA sequence

ATGGAGTTTCTTATCTTCGGTGTCTTAATCTGCCTTATTTCCACTTTCATAGCCCTCCGTCTGATTCCGGCGAGAAGAAACCCCAACCTACCTCCCGGACCACGACCTCTTCCGATCATTGGAAATCTCTTGGATCTTGGAGACAATCCCCACCAATCTCTAGCCAAACTGTCCGAGTCCTATGGCCCAATCATGTCGTTGAAACTCGGCCAACTCACCGCCGTCGTCGTATCTTCCCCGGAGACGATTCAGCAAGTCCTTCAAACCCACGACCATTTCCTATCTTTCAGAGCCGTTCCGGATGCTCTATCGCCTTACGACCACGGTCAACTAGCTTTCCCATGGATTCCCGTTTCCCCCACCTACAAAAACATTCGAAAAATCTGCAACACCCATCTTTTATCCCCCAAGACTCTCGACGCCAATCAAAACCTCCGCCGTACCAGGATAGACGACCTTCTTGCTAACATCCGACGAAGCGCTTTGAACGGGGAGGCGGTGGATATTGGGGAAGCTGTGTTTACGACGACCCTTAATTTGATTTCTTATTCGATTTGGTCGGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAATTCAAGGTCACCATGAGAGGACTGATGGAAGAAGCTGGAAAACCAAATATTAGTGACTTTTTCCCTGTGCTGAAGAGGTTGGACATGCAGGGTATAAGGAGGCGCATCACCGTTCATTTTGGAAAGATGTTTGAGATGATCGATGGGATGATTGATGAACGGTTGAAGATGCAGGAATTGCCCGATTTCAGTCCTAAAAATGATATGCTACATCATCTTCTCAACATGAGGGAGGACAACAACGAGATCCCTCTTGATAGAAACCAAATCAAACATTCAATATTGGTGTTATTTACCGGAGGAACAGATACGACTACATCAATCGTGCAATGGGCAATGACACATCTTTTGAAGAACCCAGAAACCATGTTGAAACTCAAAGAAGAACTCTTGCGAGTGATTGGGAAAGGAAATCCAGTTGAAGAATCGCACATCAACTGTTGGAAAGAGGGATAGTGAAAGAGATAGGAATAGATGAGAATAGATAGAAGCTGCAGTGCTTTATTAATGTCTACTGACTTGATGCTTACAGGGTTACACAATTCCAG

Coding sequence (CDS)

ATGGAGTTTCTTATCTTCGGTGTCTTAATCTGCCTTATTTCCACTTTCATAGCCCTCCGTCTGATTCCGGCGAGAAGAAACCCCAACCTACCTCCCGGACCACGACCTCTTCCGATCATTGGAAATCTCTTGGATCTTGGAGACAATCCCCACCAATCTCTAGCCAAACTGTCCGAGTCCTATGGCCCAATCATGTCGTTGAAACTCGGCCAACTCACCGCCGTCGTCGTATCTTCCCCGGAGACGATTCAGCAAGTCCTTCAAACCCACGACCATTTCCTATCTTTCAGAGCCGTTCCGGATGCTCTATCGCCTTACGACCACGGTCAACTAGCTTTCCCATGGATTCCCGTTTCCCCCACCTACAAAAACATTCGAAAAATCTGCAACACCCATCTTTTATCCCCCAAGACTCTCGACGCCAATCAAAACCTCCGCCGTACCAGGATAGACGACCTTCTTGCTAACATCCGACGAAGCGCTTTGAACGGGGAGGCGGTGGATATTGGGGAAGCTGTGTTTACGACGACCCTTAATTTGATTTCTTATTCGATTTGGTCGGTGGATTTGGCGGACCCAAATTCTGAAATGGCCAAACAATTCAAGGTCACCATGAGAGGACTGATGGAAGAAGCTGGAAAACCAAATATTAGTGACTTTTTCCCTGTGCTGAAGAGGTTGGACATGCAGGGTATAAGGAGGCGCATCACCGTTCATTTTGGAAAGATGTTTGAGATGATCGATGGGATGATTGATGAACGGTTGAAGATGCAGGAATTGCCCGATTTCAGTCCTAAAAATGATATGCTACATCATCTTCTCAACATGAGGGAGGACAACAACGAGATCCCTCTTGATAGAAACCAAATCAAACATTCAATATTGGTGTTATTTACCGGAGGAACAGATACGACTACATCAATCGTGCAATGGGCAATGACACATCTTTTGAAGAACCCAGAAACCATGTTGAAACTCAAAGAAGAACTCTTGCGAGTGATTGGGAAAGGAAATCCAGTTGAAGAATCGCACATCAACTGTTGGAAAGAGGGATAG

Protein sequence

MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHINCWKEG
Homology
BLAST of CmoCh02G015630 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-83
Identity = 160/343 (46.65%), Postives = 238/343 (69.39%), Query Frame = 0

Query: 9   LICLISTFIALRLIPA-----RRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGP 68
           L  +++   AL L  A     RR  NLPPGP PLP IG+L  LGD PH+SLAKLS+ +GP
Sbjct: 4   LTIILTLLFALTLYEAFSYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGP 63

Query: 69  IMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYK 128
           IMSLKLGQ+T +V+SS    ++VLQ  D   S R+VP+AL  ++  + +  W+PV+  ++
Sbjct: 64  IMSLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWR 123

Query: 129 NIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLISY 188
           ++RK+ N+++ S   LDANQ+LR  ++ +L+A  R+++ +GEAVD+G A F T+LNL+S 
Sbjct: 124 SLRKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSN 183

Query: 189 SIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKM 248
            I+S DL DP S+ AK+FK  +  +M EAGKPN+ DFFP+L+++D QGIR R+T+HFG++
Sbjct: 184 LIFSKDLTDPYSDSAKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEV 243

Query: 249 FEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTD 308
            ++  G+++ERL+ +       KND+L  LL   +++ E  +DR  I+   L LF  GTD
Sbjct: 244 LKLFGGLVNERLEQRR--SKGEKNDVLDVLLTTSQESPE-EIDRTHIERMCLDLFVAGTD 303

Query: 309 TTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           TT+S ++WAM+ +LKNP+ M K ++EL +VIG+G  +EES IN
Sbjct: 304 TTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDIN 343

BLAST of CmoCh02G015630 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.0e-80
Identity = 155/345 (44.93%), Postives = 238/345 (68.99%), Query Frame = 0

Query: 2   EFLIFGV-LICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 61
           +FL   +  +  I+ + AL    +R++ NLPPGP PLP+IGNL  LGD PH+SLAKL++ 
Sbjct: 4   DFLTIAIGFLFTITLYQALNFF-SRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKK 63

Query: 62  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 121
           +GPIM L+LGQ+T +VV+S    ++VLQ  D   S R++P+A+  +D  + +  W+PV+ 
Sbjct: 64  HGPIMGLQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVAS 123

Query: 122 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 181
            ++ +RK  N+++ S   LDANQ+LR  ++ +L+A  R+S+  G+A+D+G A F T+LNL
Sbjct: 124 RWRGLRKALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNL 183

Query: 182 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 241
           +S +++S DL DP S+ AK+FK  +  +M EAGKPN+ D+FP+L ++D QGIR+R+T+HF
Sbjct: 184 LSNTMFSKDLTDPYSDSAKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHF 243

Query: 242 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 301
           GK+ E+  G+IDERL+ ++    +  +D+L  LL   E++ E  +DR  I+   L LF  
Sbjct: 244 GKILELFGGLIDERLQQKKAKGVN--DDVLDVLLTTSEESPE-EIDRTHIQRMCLDLFVA 303

Query: 302 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
           GTDTT+S ++WAM+ +LKNPE M   + EL +VIGKG  VEE+ +
Sbjct: 304 GTDTTSSTLEWAMSEMLKNPEKMKAAQAELAQVIGKGKAVEEADL 344

BLAST of CmoCh02G015630 vs. ExPASy Swiss-Prot
Match: W8JMV1 (Cytochrome P450 76T24 OS=Catharanthus roseus OX=4058 GN=CYP76T24 PE=2 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.2e-72
Identity = 142/331 (42.90%), Postives = 217/331 (65.56%), Query Frame = 0

Query: 16  FIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAV 75
           F+  RL   +R   LPPGP P P+IGN+  LG NP+QSL KL+++YGP+MSLKLG  T V
Sbjct: 21  FLTSRLSFGKR-ARLPPGPYPFPVIGNIFQLGQNPNQSLTKLAKTYGPLMSLKLGSNTTV 80

Query: 76  VVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLS 135
           VVSSP   ++VLQ +D   S R +P A   + H + +  W+ VS  ++N+RKI   H+ +
Sbjct: 81  VVSSPAVAREVLQKNDQVFSSRIIPHAAEAHAHHKYSMVWLQVSGLWRNLRKISKEHMFA 140

Query: 136 PKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLADPNS 195
            + LDA++ LR+ ++ +L   + R + + +AV+ G+  FTT+LN IS + +SVD A   S
Sbjct: 141 AQRLDASEGLRQEKLQELHDYLVRCSTSNKAVNFGQTAFTTSLNFISSTFFSVDFAAYGS 200

Query: 196 EMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERL 255
           + +++FK  +  +++    PN++D+FPVLK +D QGI ++ T  F KMF++ D +I+ERL
Sbjct: 201 DSSQEFKDIVWRILKSYSTPNVADYFPVLKFMDPQGILQKNTFLFSKMFDIFDNIINERL 260

Query: 256 KMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTH 315
            M+   D S KND+L  LLN    NNE     N++KH +L LF GGT+TT++ ++WAM  
Sbjct: 261 MMRGSLDTSKKNDLLEALLN-HSANNESEFSLNELKHMLLDLFLGGTETTSTTLEWAMAE 320

Query: 316 LLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           LL+NPE + +++ EL +VIG+   + ES I+
Sbjct: 321 LLRNPEKLERVRAELHQVIGEKEIIHESDIS 349

BLAST of CmoCh02G015630 vs. ExPASy Swiss-Prot
Match: I3PFJ5 (Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-71
Identity = 139/321 (43.30%), Postives = 208/321 (64.80%), Query Frame = 0

Query: 30  LPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQT 89
           LPPGP+PLPIIGN+L++G  PH+S A L++ +GP++SL+LG +T +VVSS +  +++   
Sbjct: 32  LPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGPLISLRLGSVTTIVVSSADVAKEMFLK 91

Query: 90  HDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLSPKTLDANQNLRRTR 149
            DH LS R +P++++  DH +L   W+PVSP ++N RKI   HLLSP+ LDA Q  R  +
Sbjct: 92  KDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWRNFRKITAVHLLSPQRLDACQTFRHAK 151

Query: 150 IDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLADPNSEMAKQFKVTMRGLM 209
           +  L   ++  A  G+AVDIG+A FTT+LNL+S   +SV+LA   S  +++FK  +  +M
Sbjct: 152 VQQLYEYVQECAQKGQAVDIGKAAFTTSLNLLSKLFFSVELAHHKSHTSQEFKELIWNIM 211

Query: 210 EEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERLKMQELPDFS----- 269
           E+ GKPN +D+FP+L  +D  GIRRR+   F K+  +  G+I ERL     PD S     
Sbjct: 212 EDIGKPNYADYFPILGCVDPSGIRRRLACSFDKLIAVFQGIICERL----APDSSTTTTT 271

Query: 270 PKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPETML 329
             +D+L  LL + + N    L   +I H ++ +F  GTDTT+S  +W MT L++NPE M 
Sbjct: 272 TTDDVLDVLLQLFKQNE---LTMGEINHLLVDIFDAGTDTTSSTFEWVMTELIRNPEMME 331

Query: 330 KLKEELLRVIGKGNPVEESHI 346
           K +EE+ +V+GK   ++ES I
Sbjct: 332 KAQEEIKQVLGKDKQIQESDI 345

BLAST of CmoCh02G015630 vs. ExPASy Swiss-Prot
Match: P0DKI2 (Inactive cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.2e-70
Identity = 137/321 (42.68%), Postives = 206/321 (64.17%), Query Frame = 0

Query: 30  LPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQT 89
           LPPGP+PLPIIGN+L++G  PH+S A L++ +GP++SL+LG +T +VVSS +  +++   
Sbjct: 32  LPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGPLISLRLGSVTTIVVSSADVAKEMFLK 91

Query: 90  HDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLSPKTLDANQNLRRTR 149
            DH LS R +P++++  DH +L   W+PVSP ++N RKI   HLLSP+ LDA Q  R  +
Sbjct: 92  KDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWRNFRKITAVHLLSPQRLDACQTFRHAK 151

Query: 150 IDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLADPNSEMAKQFKVTMRGLM 209
           +  L   ++  A  G+AVDIG+A FTT+LNL+S   +SV+LA   S  +++FK  +  +M
Sbjct: 152 VQQLYEYVQECAQKGQAVDIGKAAFTTSLNLLSKLFFSVELAHHKSHTSQEFKELIWNIM 211

Query: 210 EEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERLKMQELPDFS----- 269
           E+ GKPN +D+FP+L  +D  GIRRR+   F K+  +   +I ERL     PD S     
Sbjct: 212 EDIGKPNYADYFPILGCVDPSGIRRRLACSFDKLIAVFQSIICERL----APDSSTATTT 271

Query: 270 PKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPETML 329
             +D+L  LL + + N    L   +I H ++ +F  GTDTT+S  +W M  L++NPE M 
Sbjct: 272 TTDDVLDVLLQLFKQNE---LTMGEINHLLVDIFDAGTDTTSSTFEWVMAELIRNPEMME 331

Query: 330 KLKEELLRVIGKGNPVEESHI 346
           K +EE+ +V+GK   ++ES I
Sbjct: 332 KAQEEIKQVLGKDKQIQESDI 345

BLAST of CmoCh02G015630 vs. ExPASy TrEMBL
Match: A0A6J1H240 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459410 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 5.6e-195
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 60
           MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES
Sbjct: 36  MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 95

Query: 61  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 120
           YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP
Sbjct: 96  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 155

Query: 121 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 180
           TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL
Sbjct: 156 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 215

Query: 181 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 240
           ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF
Sbjct: 216 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 275

Query: 241 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 300
           GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG
Sbjct: 276 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 335

Query: 301 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN
Sbjct: 336 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 381

BLAST of CmoCh02G015630 vs. ExPASy TrEMBL
Match: A0A0A0KKP6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157320 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 9.1e-137
Identity = 244/346 (70.52%), Postives = 284/346 (82.08%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIAL-RLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSE 60
           M+F  F + I LI T I L RLIPARRNP LPPGP PLPIIGNLL LGD PH SL  L++
Sbjct: 7   MDFFTFPLFIFLIFTCINLCRLIPARRNPKLPPGPTPLPIIGNLLALGDKPHLSLTNLAK 66

Query: 61  SYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVS 120
           SYGPI+SLK GQ+T VVVSSPETIQQVLQTHD+ LS+R +PDA + YDH +L  PWIP+S
Sbjct: 67  SYGPILSLKFGQVTTVVVSSPETIQQVLQTHDNVLSYRFIPDAATVYDHAELGLPWIPIS 126

Query: 121 PTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLN 180
           P YKN RKI N +LLSPK LDA++NLRR RID  L NIRR A+NGE VDIG  +F+  LN
Sbjct: 127 PNYKNHRKIFNNYLLSPKALDASRNLRRMRIDKHLDNIRRCAVNGEVVDIGTTLFSLALN 186

Query: 181 LISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVH 240
           LISYSIWS+DL D +SEM K+FK T+RG +EE G+PNISDFFPVLK++D+QG+RRR  +H
Sbjct: 187 LISYSIWSMDLVDTDSEMTKEFKATLRGSLEEMGRPNISDFFPVLKKMDVQGVRRRTAIH 246

Query: 241 FGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
           FGKM  +ID MID+RLKMQE PDF+PKNDMLHHLLNM+EDNNEIPLDRNQIKHSI VLF+
Sbjct: 247 FGKMLGLIDEMIDKRLKMQETPDFTPKNDMLHHLLNMKEDNNEIPLDRNQIKHSIFVLFS 306

Query: 301 GGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
           G +DTT S+ QWAM +LLK P+ M K KEELL VIGKGNP+EESH+
Sbjct: 307 GSSDTTGSVAQWAMAYLLKYPKVMCKAKEELLEVIGKGNPIEESHM 352

BLAST of CmoCh02G015630 vs. ExPASy TrEMBL
Match: A0A1S3ATZ2 (geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482681 PE=3 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 6.5e-135
Identity = 239/346 (69.08%), Postives = 281/346 (81.21%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIAL-RLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSE 60
           M+F  F   I LI TFI L RLIPA RNP LPPGP PLPIIGNLL LGD PH+S   L++
Sbjct: 1   MDFFTFPFFIFLIFTFITLSRLIPANRNPKLPPGPTPLPIIGNLLALGDKPHESFTNLAK 60

Query: 61  SYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVS 120
           SYGPIMSLKLGQ+TA+V S+ ETIQQVLQTHD+ LS+R +PDA + YDH +L  PWIP+S
Sbjct: 61  SYGPIMSLKLGQVTAIVFSTAETIQQVLQTHDNVLSYRFIPDAATVYDHAELGLPWIPIS 120

Query: 121 PTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLN 180
           P YKN +KI N +LLSPK LDA++NLRR RID LL NIRR A+NGE VD+G  +F+  LN
Sbjct: 121 PNYKNHKKIFNNYLLSPKALDASRNLRRMRIDKLLDNIRRCAVNGEVVDVGRTLFSMALN 180

Query: 181 LISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVH 240
           LISYSIWS+DL D +SEM K+FK T+RG +EE G+PNISDFFPVLK++D+QG+RRR  VH
Sbjct: 181 LISYSIWSMDLVDTDSEMIKEFKTTLRGSLEEMGRPNISDFFPVLKKMDIQGVRRRTAVH 240

Query: 241 FGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
           FGK+  +ID MID+RLKMQE PDF+PKNDMLHHLLNM+EDNN+IPLDRNQIKHSI VLFT
Sbjct: 241 FGKILGLIDEMIDKRLKMQESPDFTPKNDMLHHLLNMKEDNNQIPLDRNQIKHSIFVLFT 300

Query: 301 GGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
            G D   SI QWAM +LLKNP+ M K KEEL+ VIGKGN +EESH+
Sbjct: 301 AGPDAAGSIAQWAMAYLLKNPKVMCKAKEELMEVIGKGNSIEESHM 346

BLAST of CmoCh02G015630 vs. ExPASy TrEMBL
Match: A0A5A7THK3 (Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003310 PE=3 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.4e-134
Identity = 238/346 (68.79%), Postives = 281/346 (81.21%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIAL-RLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSE 60
           M+F  F   I LI TFI L RLIPA RNP LPPGP PLPIIGNLL LGD PH+S   L++
Sbjct: 1   MDFFTFPFFIFLIFTFITLSRLIPANRNPKLPPGPTPLPIIGNLLALGDKPHESFTNLAK 60

Query: 61  SYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVS 120
           SYGPIMSLKLGQ+TA+V S+ ETIQQVLQTHD+ LS+R +PDA + YDH +L  PWIP++
Sbjct: 61  SYGPIMSLKLGQVTAIVFSTAETIQQVLQTHDNVLSYRFIPDAATVYDHAELGLPWIPIT 120

Query: 121 PTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLN 180
           P YKN +KI N +LLSPK LDA++NLRR RID LL NIRR A+NGE VD+G  +F+  LN
Sbjct: 121 PNYKNHKKIFNNYLLSPKALDASRNLRRMRIDKLLDNIRRCAVNGEVVDVGRTLFSMALN 180

Query: 181 LISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVH 240
           LISYSIWS+DL D +SEM K+FK T+RG +EE G+PNISDFFPVLK++D+QG+RRR  VH
Sbjct: 181 LISYSIWSMDLVDTDSEMIKEFKTTLRGSLEEMGRPNISDFFPVLKKMDIQGVRRRTAVH 240

Query: 241 FGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
           FGK+  +ID MID+RLKMQE PDF+PKNDMLHHLLNM+EDNN+IPLDRNQIKHSI VLFT
Sbjct: 241 FGKILGLIDEMIDKRLKMQESPDFTPKNDMLHHLLNMKEDNNQIPLDRNQIKHSIFVLFT 300

Query: 301 GGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
            G D   SI QWAM +LLKNP+ M K KEEL+ VIGKGN +EESH+
Sbjct: 301 AGPDAAGSIAQWAMAYLLKNPKVMCKAKEELMEVIGKGNSIEESHM 346

BLAST of CmoCh02G015630 vs. ExPASy TrEMBL
Match: A0A6J1DZD0 (geraniol 8-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025984 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 5.0e-127
Identity = 234/345 (67.83%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 3   FLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYG 62
           ++I   ++CLI + I +     RRNP LPPGPR LPIIGNLLDLGD PH+SLA+L+ ++G
Sbjct: 4   WMIPSCMLCLIFSVILIS-TRRRRNPKLPPGPRRLPIIGNLLDLGDKPHKSLARLANAHG 63

Query: 63  PIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTY 122
           PIMSLKLGQ+TAVVVSSPE I+ VLQTHDH  S RAVPDA + +DH QLA PWIPVSP +
Sbjct: 64  PIMSLKLGQVTAVVVSSPEMIRHVLQTHDHITSSRAVPDAAAVFDHHQLALPWIPVSPIW 123

Query: 123 KNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLIS 182
           +NIR+I NT L +P  LDAN+ LRR +++DLLANIR+SA++G AVDIG AVF TTLN++S
Sbjct: 124 RNIRRIYNTRLFAPNILDANEILRRAKLEDLLANIRQSAVSGAAVDIGGAVFATTLNMMS 183

Query: 183 YSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGK 242
            SIWSVDLADPNSEMAK+FK T+RG++EE+GKPNISDFFPVLK +D++G+RRR  VH  K
Sbjct: 184 NSIWSVDLADPNSEMAKEFKETLRGVLEESGKPNISDFFPVLKMMDIEGVRRRNAVHLRK 243

Query: 243 MFEMIDGMIDERLK-MQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGG 302
           M ++ID MID RL  M+E PDF+PKND+LHHLLNM E N EI LDR+QIKHSILVLF  G
Sbjct: 244 MLDLIDKMIDRRLGIMRESPDFAPKNDVLHHLLNMGEHNGEISLDRSQIKHSILVLFIAG 303

Query: 303 TDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
            DTTT++VQW MT+LLK+P++M K KEEL RVIGKGNP+EES I+
Sbjct: 304 IDTTTAMVQWTMTYLLKDPKSMSKAKEELQRVIGKGNPMEESDID 347

BLAST of CmoCh02G015630 vs. NCBI nr
Match: XP_022958073.1 (geraniol 8-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 689.9 bits (1779), Expect = 1.2e-194
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 60
           MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES
Sbjct: 36  MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 95

Query: 61  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 120
           YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP
Sbjct: 96  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 155

Query: 121 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 180
           TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL
Sbjct: 156 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 215

Query: 181 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 240
           ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF
Sbjct: 216 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 275

Query: 241 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 300
           GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG
Sbjct: 276 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 335

Query: 301 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN
Sbjct: 336 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 381

BLAST of CmoCh02G015630 vs. NCBI nr
Match: XP_023532730.1 (geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.6 bits (1747), Expect = 5.9e-191
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 60
           MEFLIFG +ICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKL+ES
Sbjct: 36  MEFLIFGFIICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLAES 95

Query: 61  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 120
           YGPIMSLKLGQ+TAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP
Sbjct: 96  YGPIMSLKLGQVTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 155

Query: 121 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 180
           TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL
Sbjct: 156 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 215

Query: 181 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 240
           ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF
Sbjct: 216 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 275

Query: 241 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 300
           GKMFEMIDG IDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG
Sbjct: 276 GKMFEMIDGKIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTG 335

Query: 301 GTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           GT+TTTSIVQWAMTHLLKNPETM+KLKEEL RVIGKGNPVEESHIN
Sbjct: 336 GTETTTSIVQWAMTHLLKNPETMVKLKEELSRVIGKGNPVEESHIN 381

BLAST of CmoCh02G015630 vs. NCBI nr
Match: KAG6606159.1 (Geraniol 8-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.8 bits (1514), Expect = 6.2e-164
Identity = 295/299 (98.66%), Postives = 296/299 (99.00%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 60
           MEFLIFG +ICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES
Sbjct: 36  MEFLIFGFIICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSES 95

Query: 61  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 120
           YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP
Sbjct: 96  YGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSP 155

Query: 121 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 180
           TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL
Sbjct: 156 TYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNL 215

Query: 181 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 240
           ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF
Sbjct: 216 ISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHF 275

Query: 241 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
           GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILV  T
Sbjct: 276 GKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVRST 334

BLAST of CmoCh02G015630 vs. NCBI nr
Match: XP_038875939.1 (geraniol 8-hydroxylase-like [Benincasa hispida])

HSP 1 Score: 518.1 bits (1333), Expect = 6.0e-143
Identity = 255/346 (73.70%), Postives = 296/346 (85.55%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIAL-RLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSE 60
           M+   F  LI LIS FI L RLI  RRNP LPPGP PLPIIGNLLDLG NPHQSLAKL++
Sbjct: 1   MDSFSFCFLISLISIFITLRRLITTRRNPKLPPGPTPLPIIGNLLDLGHNPHQSLAKLAK 60

Query: 61  SYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVS 120
           SYGPIMSLKLGQ+TAVVVSSPETIQQVLQTHDH LS+RAVPDALS YDHGQ   PWIP+ 
Sbjct: 61  SYGPIMSLKLGQVTAVVVSSPETIQQVLQTHDHVLSYRAVPDALSAYDHGQQGLPWIPIF 120

Query: 121 PTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLN 180
           PTYKNIR+I N +LLSPKTL+ ++NLRR RIDDL+ N+RRSA+NGEAVDI   VF TTLN
Sbjct: 121 PTYKNIRRIFNNYLLSPKTLNTSRNLRRMRIDDLVDNVRRSAVNGEAVDIRGVVFATTLN 180

Query: 181 LISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVH 240
           LISYSIWS+DL DPNS+MAK+F+ T+RG++EEAG+ NISDFFPVLK++D+QG+RRRITV 
Sbjct: 181 LISYSIWSMDLVDPNSKMAKEFEETLRGIIEEAGRANISDFFPVLKKMDIQGVRRRITVR 240

Query: 241 FGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
            G M ++ID  ID+RLKMQE PDF+ +NDMLHHLLN REDNNEIPLDRNQIKHSI VLF 
Sbjct: 241 LGIMLDLIDETIDQRLKMQESPDFTQQNDMLHHLLNTREDNNEIPLDRNQIKHSIFVLFI 300

Query: 301 GGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
           G T+TT S+V+WAM H+L+NP+ M + KEE+ +VIGKGNP+EESHI
Sbjct: 301 GSTETTASVVEWAMAHILRNPKVMWRAKEEMFQVIGKGNPIEESHI 346

BLAST of CmoCh02G015630 vs. NCBI nr
Match: XP_004147602.1 (geraniol 8-hydroxylase [Cucumis sativus] >KGN50173.1 hypothetical protein Csa_000164 [Cucumis sativus])

HSP 1 Score: 496.5 bits (1277), Expect = 1.9e-136
Identity = 244/346 (70.52%), Postives = 284/346 (82.08%), Query Frame = 0

Query: 1   MEFLIFGVLICLISTFIAL-RLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSE 60
           M+F  F + I LI T I L RLIPARRNP LPPGP PLPIIGNLL LGD PH SL  L++
Sbjct: 7   MDFFTFPLFIFLIFTCINLCRLIPARRNPKLPPGPTPLPIIGNLLALGDKPHLSLTNLAK 66

Query: 61  SYGPIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVS 120
           SYGPI+SLK GQ+T VVVSSPETIQQVLQTHD+ LS+R +PDA + YDH +L  PWIP+S
Sbjct: 67  SYGPILSLKFGQVTTVVVSSPETIQQVLQTHDNVLSYRFIPDAATVYDHAELGLPWIPIS 126

Query: 121 PTYKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLN 180
           P YKN RKI N +LLSPK LDA++NLRR RID  L NIRR A+NGE VDIG  +F+  LN
Sbjct: 127 PNYKNHRKIFNNYLLSPKALDASRNLRRMRIDKHLDNIRRCAVNGEVVDIGTTLFSLALN 186

Query: 181 LISYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVH 240
           LISYSIWS+DL D +SEM K+FK T+RG +EE G+PNISDFFPVLK++D+QG+RRR  +H
Sbjct: 187 LISYSIWSMDLVDTDSEMTKEFKATLRGSLEEMGRPNISDFFPVLKKMDVQGVRRRTAIH 246

Query: 241 FGKMFEMIDGMIDERLKMQELPDFSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFT 300
           FGKM  +ID MID+RLKMQE PDF+PKNDMLHHLLNM+EDNNEIPLDRNQIKHSI VLF+
Sbjct: 247 FGKMLGLIDEMIDKRLKMQETPDFTPKNDMLHHLLNMKEDNNEIPLDRNQIKHSIFVLFS 306

Query: 301 GGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHI 346
           G +DTT S+ QWAM +LLK P+ M K KEELL VIGKGNP+EESH+
Sbjct: 307 GSSDTTGSVAQWAMAYLLKYPKVMCKAKEELLEVIGKGNPIEESHM 352

BLAST of CmoCh02G015630 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 258.5 bits (659), Expect = 8.0e-69
Identity = 138/323 (42.72%), Postives = 209/323 (64.71%), Query Frame = 0

Query: 30  LPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQT 89
           LPPGP  LP++GN+  LG NPH+SLA  S++YGPIMSLKLG+LTAVV+SSPE  ++ L+T
Sbjct: 38  LPPGPPILPLVGNIFQLGFNPHRSLAAFSKTYGPIMSLKLGRLTAVVISSPEAAKEALRT 97

Query: 90  HDHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLSPKTLDANQNLRRTR 149
           HDH +S R   DAL  +DH + +  WIP S  ++ ++K    +LLSP+ LDA Q+LR  +
Sbjct: 98  HDHVMSARTFNDALRAFDHHKHSIVWIPPSARWRFLKKTITKYLLSPQNLDAIQSLRMRK 157

Query: 150 IDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLA--DPNSEMAKQFKVTMRG 209
           +++L++ +      GEA+D+  A F T+ N+IS +++SVDLA  D NS  + +F  T+  
Sbjct: 158 VEELVSLVNEFRERGEAIDLARASFVTSFNIISNALFSVDLATYDSNSS-SYEFHNTVVH 217

Query: 210 LMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERL-----KMQELPD 269
           L + AG PN+ D+F  ++ LD+QG R++  +   K+F +    ID RL     + ++ P 
Sbjct: 218 LTDIAGIPNVGDYFQYMRFLDLQGTRKKAVLCIEKLFRVFQEFIDARLAKRFSRTEKEPK 277

Query: 270 FSPKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPET 329
            +   DML  LL++ +  NE  L  N +KH +L +F  GTDT +S ++WAMT L ++ E 
Sbjct: 278 EASSIDMLDSLLDLTQ-QNEAELTMNDLKHLLLDVFVAGTDTNSSTMEWAMTELFRSTEK 337

Query: 330 MLKLKEELLRVIGKGNPVEESHI 346
           M+K + E+ +VIG+   V+ES I
Sbjct: 338 MVKAQSEIRQVIGQNGFVQESDI 358

BLAST of CmoCh02G015630 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 248.1 bits (632), Expect = 1.1e-65
Identity = 138/348 (39.66%), Postives = 221/348 (63.51%), Query Frame = 0

Query: 8   VLICLIST-FIALRLIPARRN----PNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYG 67
           +L C IS+ F+      +RR+      LPPGP  LPIIGN+  +G NPH S A L++ YG
Sbjct: 11  LLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYG 70

Query: 68  PIMSLKLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPT- 127
           PIMSLK G L +VV++SPE  ++VL+THD  LS R   D++  + H +++  W+P S   
Sbjct: 71  PIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSSAR 130

Query: 128 YKNIRKICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLI 187
           ++ +RK+  T + SP+  +A + LR  ++ +L++ +  S+   EAVDI  A +TT LN+I
Sbjct: 131 WRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNII 190

Query: 188 SYSIWSVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFG 247
           S  ++SVDL   +S+ + +F+ T+ G ME AGKP+ +++FP +  LD+QG R+ +     
Sbjct: 191 SNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTE 250

Query: 248 KMFEMIDGMIDERLKMQELPDFS---PKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLF 307
           ++F +  G +D ++  + L ++S      D L  LL + E  +E  LD N I+H +L +F
Sbjct: 251 RLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNE-GDEAELDNNDIEHLLLDMF 310

Query: 308 TGGTDTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           T GTDT++S ++WAM  LL+NP+TM+K + E+ RV+G+ + V+ES I+
Sbjct: 311 TAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDIS 357

BLAST of CmoCh02G015630 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 141/344 (40.99%), Postives = 213/344 (61.92%), Query Frame = 0

Query: 8   VLICLISTFIALRLIPARRNPNLPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSL 67
           VL   I  F   R   +R+    PPGP  LPIIGN+  +G NPH S A LS++YGPIMSL
Sbjct: 16  VLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGPIMSL 75

Query: 68  KLGQLTAVVVSSPETIQQVLQTHDHFLSFRAVPDALSPYDHGQLAFPWIPVSPT-YKNIR 127
           K G L  VVV+SPE  ++VL+T+D  LS R   +++   +H +++  W+P S + ++ +R
Sbjct: 76  KFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSSRWRLLR 135

Query: 128 KICNTHLLSPKTLDANQNLRRTRIDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIW 187
           K+  T L SP+ ++A + LR  ++ +L++ +  S+   EAVDI  A F T LN+IS  ++
Sbjct: 136 KLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITALNIISNILF 195

Query: 188 SVDLADPNSEMAKQFKVTMRGLMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEM 247
           SVDL + +S  +  F+ T+ G+ME  G P+ ++FFP L  LD+QG R+ +     ++F++
Sbjct: 196 SVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLKACSERLFKV 255

Query: 248 IDGMIDERLKMQELPDFSPKN----DMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGT 307
             G ID +L  + L D + K+    D +  LL++ E  +E  L+ N I H +L LF  GT
Sbjct: 256 FRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTE-GDEAELNTNDIVHLLLDLFGAGT 315

Query: 308 DTTTSIVQWAMTHLLKNPETMLKLKEELLRVIGKGNPVEESHIN 347
           DT +S V+WAM  LL+NPETM+K + E+  VIG+   VEES I+
Sbjct: 316 DTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDIS 358

BLAST of CmoCh02G015630 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 235.7 bits (600), Expect = 5.6e-62
Identity = 130/322 (40.37%), Postives = 207/322 (64.29%), Query Frame = 0

Query: 30  LPPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQT 89
           LPPGP  LPIIGN+  +G +PH+S A+LS++YGP+MSLKLG L  VV++SPE  ++VL+T
Sbjct: 38  LPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRT 97

Query: 90  HDHFLSFRAVPDALSPYDHGQLAFPWIPVSPT-YKNIRKICNTHLLSPKTLDANQNLRRT 149
           HD  LS R+  +A+   +H   +  W+P S   ++ +R++  T LLSP+ ++A + LR  
Sbjct: 98  HDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATKALRMN 157

Query: 150 RIDDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLADPNSEMA-KQFKVTMRG 209
           ++ +L++ I  S+   E+VDI    F TTLN+IS  ++SVDL   N++ +    + T+  
Sbjct: 158 KVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQDTVIS 217

Query: 210 LMEEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERL---KMQELPDFS 269
           +M+ AG P+ +++FP L+ LD+QG  +   V   ++  +  G ID ++     Q  P   
Sbjct: 218 VMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDV 277

Query: 270 PKNDMLHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPETML 329
            KND + +LL+ + D +E+ +  + I+H +L +FT GTDT++S ++WAMT LLKNP+TM 
Sbjct: 278 SKNDFVDNLLDYKGDESELSI--SDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMA 337

Query: 330 KLKEELLRVIGKGNPVEESHIN 347
           K + E+  VIG+   VEES I+
Sbjct: 338 KAQAEIDCVIGQNGIVEESDIS 357

BLAST of CmoCh02G015630 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 127/316 (40.19%), Postives = 191/316 (60.44%), Query Frame = 0

Query: 31  PPGPRPLPIIGNLLDLGDNPHQSLAKLSESYGPIMSLKLGQLTAVVVSSPETIQQVLQTH 90
           PPGP  L ++ N+L   + PH+SLA LS  YG +MS KLG LT VV+SSPET ++VL+TH
Sbjct: 34  PPGPSKLSLLRNILQTVEKPHRSLADLSRIYGSVMSFKLGCLTTVVISSPETAKEVLKTH 93

Query: 91  DHFLSFRAVPDALSPYDHGQLAFPWIPVSPTYKNIRKICNTHLLSPKTLDANQNLRRTRI 150
           DH LS+R   D +    H +L+  WIP    ++ +RKI    L S + L+A   +R  ++
Sbjct: 94  DHVLSYRVSSDPVRAAGHHELSLLWIPPLARWRFLRKITRNQLFSTQRLEATSAIRTRKV 153

Query: 151 DDLLANIRRSALNGEAVDIGEAVFTTTLNLISYSIWSVDLAD-PNSEMAKQFKVTMRGLM 210
            +L+  + +     EAV+I  A F T+LN+IS +++S +LA+  +S+    F+  +  +M
Sbjct: 154 QELMNFVNKCCERREAVNISRASFITSLNIISNALFSTNLANFDDSKTFHDFQNVVIRMM 213

Query: 211 EEAGKPNISDFFPVLKRLDMQGIRRRITVHFGKMFEMIDGMIDERLKMQELPDFSPKNDM 270
           E +GKPN++DFFP L  LD+QG R+   +   K+F +  G ID +            NDM
Sbjct: 214 EISGKPNLADFFPFLGFLDLQGARKEARLLMHKLFRVFQGFIDTKRSSTS----RNNNDM 273

Query: 271 LHHLLNMREDNNEIPLDRNQIKHSILVLFTGGTDTTTSIVQWAMTHLLKNPETMLKLKEE 330
           L  LL++     E  LD N IKH +L LF  G DT++S V+WAM  LL+NP+ ++K++EE
Sbjct: 274 LDSLLDIAH-KKESELDDNNIKHLLLDLFLAGVDTSSSAVEWAMAELLRNPKMIVKVQEE 333

Query: 331 LLRVIGKGNPVEESHI 346
           + +VIG    V++  I
Sbjct: 334 IRQVIGLKGTVQDLDI 344

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VWZ71.1e-8346.65Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1[more]
D1MI462.0e-8044.93Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1[more]
W8JMV11.2e-7242.90Cytochrome P450 76T24 OS=Catharanthus roseus OX=4058 GN=CYP76T24 PE=2 SV=1[more]
I3PFJ51.7e-7143.30Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1[more]
P0DKI23.2e-7042.68Inactive cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H2405.6e-195100.00geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459410 PE=3 S... [more]
A0A0A0KKP69.1e-13770.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157320 PE=3 SV=1[more]
A0A1S3ATZ26.5e-13569.08geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482681 PE=3 SV=1[more]
A0A5A7THK31.4e-13468.79Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A6J1DZD05.0e-12767.83geraniol 8-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111025984 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_022958073.11.2e-194100.00geraniol 8-hydroxylase-like [Cucurbita moschata][more]
XP_023532730.15.9e-19197.69geraniol 8-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
KAG6606159.16.2e-16498.66Geraniol 8-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038875939.16.0e-14373.70geraniol 8-hydroxylase-like [Benincasa hispida][more]
XP_004147602.11.9e-13670.52geraniol 8-hydroxylase [Cucumis sativus] >KGN50173.1 hypothetical protein Csa_00... [more]
Match NameE-valueIdentityDescription
AT2G45580.18.0e-6942.72cytochrome P450, family 76, subfamily C, polypeptide 3 [more]
AT2G45550.11.1e-6539.66cytochrome P450, family 76, subfamily C, polypeptide 4 [more]
AT2G45570.12.0e-6440.99cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
AT2G45560.15.6e-6240.37cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT3G61040.12.3e-6040.19cytochrome P450, family 76, subfamily C, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 58..77
score: 36.8
coord: 309..335
score: 36.07
coord: 289..306
score: 32.97
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..345
e-value: 7.3E-49
score: 166.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 22..349
e-value: 1.7E-61
score: 210.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 25..346
NoneNo IPR availablePANTHERPTHR47950:SF4CYTOCHROME P450 FAMILY MONOOXYGENASEcoord: 8..346
NoneNo IPR availablePANTHERPTHR47950CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATEDcoord: 8..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G015630.1CmoCh02G015630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen