CmoCh02G012150 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G012150
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr02: 7309118 .. 7311110 (-)
RNA-Seq ExpressionCmoCh02G012150
SyntenyCmoCh02G012150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTAGGTGTGGCCATGGAGCTCATCAGAAGCGCAGCCCTATTCAAGTCTTTGTTCTTCACTGCTTATGTGACATTGATTGGTCTGTTGGGGCTAAGTCTTGCAGCTCAGGGATCCAGCATTACAGCCATGTACTTGTTGGGAGATTCTTCTGTGGATTGCGGCTTAAACGCTCTGTTTTACCCTCTTCTTCATCGCAATTTCTCCATCATTCCTTGCGACTCCGATGGCACCACCCTTCTTCCTCATCTTCTTGGTTAGTACCCTCCCTCTTGCTTGCCTATTCACTCTTTGAATTATGTTTCCAACTCTCTCAAAAATCGATTTTGAAATATTTAATAATCTATAAAGTTATAAGATCCTATATTGATTAGAGAGGAGAACAAAACCTTTTTTGAAAGGGTGAGAAAACTTCTACCTAACAGACCCGAAGAAAAGTCCAATATCTATTGATAGTATTACAATTGTGATGTCCCAAGTTGGTTGGAGAAAGAACAAACAATTGTGATGTCCCAAGTTGGTTGGAGAAAGAACAAAATACTATCTATAAGGGTGTGAAAATCTTCCCGTTTTAAAGTTTTGAGGAGAACACGAAAGGGTTGTTACAAATGGTATAAGAGCCAGACACCGGATAATGTGTCAGCCTTCTCGCTGTTCCCCAAAGGGGGTAGACACGAGATAATGTGCCAATAAGGACACTTGCCCCATTGGGATGGATTTCGTGCCAACCTTCTCGATGTTCCCCAAATGGGATAGATACGAAACGGTGTGCCCGTAAGGACACTAGCCTCAAAGGGGTGGATTTAGTGGCGGTCCCACATCGATTGGAGAAAGGAACGAGTGCCAGCGAAGAGGCTGGGCCTCGAATGAGGTGGATTGTGATGTCCCGAAGAGAACAAAAACACCATTTGTAAGGGTGTAGAAACCTTCCACGAGAGGGAAGTTACTCTTTTACTTTTAATAAAACATTATGTAAAATGTACCTTATTCTTGCAGCTGAGGAGATGGGCGTTCCTCACTCCCAACCTTGGTACAATCAGAATGGTTCCATTGAGGCGATCCTAAATGGCCTCAGCTTTGGGTCGCCACAGGCAACGATCGTGAGCGGCGGCCAAAGCCATCAATCTCTCAACCAACAGCTACGCCAAGTACTCGACACCCTGCAGCTCTTGCGCCTACAACTCGGACTAGACACTGCTCGCCATTTCATCCAATCTTCCCTCTTCTACTTCTCATTTGGCGAGGTCGATTTTATTCTCGACTCATCCAGAGGCAAAAACAACTTGAAATTTCCTCAGCTGCTGGCCTCACAAATGTCGGTCGCTATACGAAATCTCCACGAAGCAGGGGCGAAGAAGATCGTATGCATGGGAATACTGCCATTGGGATGTACTCCTCGAGTTCTATCTCAATGGCGTGATTCCCCTGCGGCCGTATATGACGAGAAAGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAATTACAATGAACTGATGGAGAAGCAGATCGTGATGCTGAACGAACAGCTTGGTGATGCTCGAATGGTTTTCTGCGACGCGTACAAAGGAATGATGGAGATTATTGGCAACCCGAGGCGATACGGTATGGATTACTCGATTCTGAGATTTGTGTGGATTTGGGTAAAGAACAACGAGATTTGATTTGAGATGATGAATTTTTAGGATTTGAGGAGTCGAGGAGTGCGTGTTGTGGACTTGGATCATACAATGCGAGCATAGGATGTGTGGCGAAGGAGATTGCGTGCGGCCGAGTCCGGAGGCATGTGTGGTGGGATCTGTACAATCCGACGGGGGCTGTGAATTCGTTGCTTGCGAATTCTGCTTGGCGCGATCAGCCATTCTCCAGCTTCTGCCGTCCGTCCACCATTGGAGACTTGGTGAAATGACTGCAGATTCAGATTCAATTTTCCATGTAATTTAACTCGAATAAACTTCAATTTCACTCGTATGAACAGAGCAAAAACAGG

mRNA sequence

ATGACTTTAGGTGTGGCCATGGAGCTCATCAGAAGCGCAGCCCTATTCAAGTCTTTGTTCTTCACTGCTTATGTGACATTGATTGGTCTGTTGGGGCTAAGTCTTGCAGCTCAGGGATCCAGCATTACAGCCATGTACTTGTTGGGAGATTCTTCTGTGGATTGCGGCTTAAACGCTCTGTTTTACCCTCTTCTTCATCGCAATTTCTCCATCATTCCTTGCGACTCCGATGGCACCACCCTTCTTCCTCATCTTCTTGCTGAGGAGATGGGCGTTCCTCACTCCCAACCTTGGTACAATCAGAATGGTTCCATTGAGGCGATCCTAAATGGCCTCAGCTTTGGGTCGCCACAGGCAACGATCGTGAGCGGCGGCCAAAGCCATCAATCTCTCAACCAACAGCTACGCCAAGTACTCGACACCCTGCAGCTCTTGCGCCTACAACTCGGACTAGACACTGCTCGCCATTTCATCCAATCTTCCCTCTTCTACTTCTCATTTGGCGAGGTCGATTTTATTCTCGACTCATCCAGAGGCAAAAACAACTTGAAATTTCCTCAGCTGCTGGCCTCACAAATGTCGGTCGCTATACGAAATCTCCACGAAGCAGGGGCGAAGAAGATCGTATGCATGGGAATACTGCCATTGGGATGTACTCCTCGAGTTCTATCTCAATGGCGTGATTCCCCTGCGGCCGTATATGACGAGAAAGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAATTACAATGAACTGATGGAGAAGCAGATCGTGATGCTGAACGAACAGCTTGGTGATGCTCGAATGGTTTTCTGCGACGCGTACAAAGGAATGATGGAGATTATTGGCAACCCGAGGCGATACGGATTTGAGGAGTCGAGGAGTGCGTGTTGTGGACTTGGATCATACAATGCGAGCATAGGATGTGTGGCGAAGGAGATTGCGTGCGGCCGAGTCCGGAGGCATGTGTGGTGGGATCTGTACAATCCGACGGGGGCTGTGAATTCGTTGCTTGCGAATTCTGCTTGGCGCGATCAGCCATTCTCCAGCTTCTGCCGTCCGTCCACCATTGGAGACTTGGTGAAATGACTGCAGATTCAGATTCAATTTTCCATGTAATTTAACTCGAATAAACTTCAATTTCACTCGTATGAACAGAGCAAAAACAGG

Coding sequence (CDS)

ATGACTTTAGGTGTGGCCATGGAGCTCATCAGAAGCGCAGCCCTATTCAAGTCTTTGTTCTTCACTGCTTATGTGACATTGATTGGTCTGTTGGGGCTAAGTCTTGCAGCTCAGGGATCCAGCATTACAGCCATGTACTTGTTGGGAGATTCTTCTGTGGATTGCGGCTTAAACGCTCTGTTTTACCCTCTTCTTCATCGCAATTTCTCCATCATTCCTTGCGACTCCGATGGCACCACCCTTCTTCCTCATCTTCTTGCTGAGGAGATGGGCGTTCCTCACTCCCAACCTTGGTACAATCAGAATGGTTCCATTGAGGCGATCCTAAATGGCCTCAGCTTTGGGTCGCCACAGGCAACGATCGTGAGCGGCGGCCAAAGCCATCAATCTCTCAACCAACAGCTACGCCAAGTACTCGACACCCTGCAGCTCTTGCGCCTACAACTCGGACTAGACACTGCTCGCCATTTCATCCAATCTTCCCTCTTCTACTTCTCATTTGGCGAGGTCGATTTTATTCTCGACTCATCCAGAGGCAAAAACAACTTGAAATTTCCTCAGCTGCTGGCCTCACAAATGTCGGTCGCTATACGAAATCTCCACGAAGCAGGGGCGAAGAAGATCGTATGCATGGGAATACTGCCATTGGGATGTACTCCTCGAGTTCTATCTCAATGGCGTGATTCCCCTGCGGCCGTATATGACGAGAAAGGATGTGTGAAAGAGATGAATGAGTTGGTTGTGAATTACAATGAACTGATGGAGAAGCAGATCGTGATGCTGAACGAACAGCTTGGTGATGCTCGAATGGTTTTCTGCGACGCGTACAAAGGAATGATGGAGATTATTGGCAACCCGAGGCGATACGGATTTGAGGAGTCGAGGAGTGCGTGTTGTGGACTTGGATCATACAATGCGAGCATAGGATGTGTGGCGAAGGAGATTGCGTGCGGCCGAGTCCGGAGGCATGTGTGGTGGGATCTGTACAATCCGACGGGGGCTGTGAATTCGTTGCTTGCGAATTCTGCTTGGCGCGATCAGCCATTCTCCAGCTTCTGCCGTCCGTCCACCATTGGAGACTTGGTGAAATGA

Protein sequence

MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLVK
Homology
BLAST of CmoCh02G012150 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 7.1e-33
Identity = 99/332 (29.82%), Postives = 153/332 (46.08%), Query Frame = 0

Query: 42  ITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD---------SDGTTLLPHLLAEEMGV 101
           + AM++LGDS VD G N     +   NF     D         S+G T +  LLA  + +
Sbjct: 39  VPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFI-DLLARLLEI 98

Query: 102 PHSQPWYNQNGSIEAILNGLSFGSPQATI--VSGGQ--SHQSLNQQLRQVLDTLQLLRLQ 161
           P   P+ +   S   IL G+++ S  A I  VSG       SLNQQ+  +  TL  LR  
Sbjct: 99  PSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTM 158

Query: 162 LGLDTARHFIQSSLFYFSFGEVDFI--------LDSSRGKNNLKFPQLLASQMSVAIRNL 221
           +       ++  SL    FG  D+I         DSS       F  LL SQ +  +  L
Sbjct: 159 MSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTL 218

Query: 222 HEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVM 281
           +  G +KI   G+ PLGC P   ++    P        CV  +N+++  +N+ ++  +  
Sbjct: 219 YSLGLRKIFIPGVAPLGCIPNQRARGISPP------DRCVDSVNQILGTFNQGLKSLVDQ 278

Query: 282 LNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRV 341
           LN++   A  V+ + Y  + +I+ NP  YGF     ACCG+G     I C+  +  C   
Sbjct: 279 LNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNR 338

Query: 342 RRHVWWDLYNPTGAVNSLLANSAWRDQPFSSF 353
            ++V+WD ++PT   NS+LA  A+   P  ++
Sbjct: 339 NQYVFWDAFHPTQTANSILARRAFYGPPSDAY 363

BLAST of CmoCh02G012150 vs. ExPASy Swiss-Prot
Match: Q8L5Z1 (GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-27
Identity = 90/341 (26.39%), Postives = 160/341 (46.92%), Query Frame = 0

Query: 33  LSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD-SDGTT-------LLPH 92
           +S   Q + +  +++ GDS VD G N     L   N+     D   GTT           
Sbjct: 23  VSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFPQGTTGRFTNGRTYVD 82

Query: 93  LLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATI--VSGGQ--SHQSLNQQLRQVLD 152
            LA+ +G  +  P Y++    +AIL G +F S  A I   +G    +H S+NQQ+     
Sbjct: 83  ALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNLGAHTSMNQQVELYTT 142

Query: 153 TLQLLRLQLGLDT--ARHFIQSSLFYFSFGEVDFI--------LDSSRGKNNLKFPQLLA 212
            +Q +      DT   + ++   +FY   G  D++          +S   N+  F + L 
Sbjct: 143 AVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYSTSTNYNDKTFAESLI 202

Query: 213 SQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNY 272
              +  +  L++ GA+K++  G+  +GC P  L+++ +   +      C +++N  +V +
Sbjct: 203 KNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST---GRCNEKINNAIVVF 262

Query: 273 NELMEKQIVMLNE-QLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIG 332
           N  ++K +  LN+ QL  A+ V+ D+YK   ++  N   YGFE     CCG+G  N  I 
Sbjct: 263 NTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVVDKGCCGVGRNNGQIT 322

Query: 333 CVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFS 351
           C+  +  C    ++++WD ++PT   N LLA S +  + ++
Sbjct: 323 CLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSRAYT 359

BLAST of CmoCh02G012150 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.8e-26
Identity = 87/360 (24.17%), Postives = 170/360 (47.22%), Query Frame = 0

Query: 23  AYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCG-LNALFYPLLHRNFSIIPCDSDG--- 82
           A   +I     +  A  SS+   Y+ GDS  + G  N L Y L   +F     D  G   
Sbjct: 7   ALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKA 66

Query: 83  ------TTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQ----SH 142
                    +  +++ ++G+    P+ + + + +A L+G+++ S  A I++         
Sbjct: 67  TGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGIYFIQR 126

Query: 143 QSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI-------LDSSRGKN 202
            + N Q+     T +++R ++G   A   +  ++++   G  D++       +   +   
Sbjct: 127 LTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYT 186

Query: 203 NLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTP--RVLSQWRDSPAAVYDEKGC 262
           + +F +LL S +   +  +++ GA+K++  G+ PLGC P  RV S+ R           C
Sbjct: 187 HDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTR----------MC 246

Query: 263 VKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACC 322
           +  +NE V+ +N   +K ++ LN++L  A+  F D Y  ++++I NP  YGF+ + ++CC
Sbjct: 247 LNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCC 306

Query: 323 GLGSYNASIG--CVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPST 358
            +   + S+G  C+     C   +  V+WD ++P+ + N +LA     D  FSS    S+
Sbjct: 307 NV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILA-----DHLFSSLLSSSS 347

BLAST of CmoCh02G012150 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 5.0e-26
Identity = 93/343 (27.11%), Postives = 154/343 (44.90%), Query Frame = 0

Query: 44  AMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD----------SDGTTLLPHLLAEEMGVP 103
           AM++ GDS VD G N     L   N+     D          S+G T++   + E +G+P
Sbjct: 49  AMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIV-DFIGELLGLP 108

Query: 104 HSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQ----SLNQQLRQVLDTLQLLRLQL 163
               + +       IL+G+++ S    I+     H     S+ +Q+     TL  +   +
Sbjct: 109 EIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSM 168

Query: 164 GLDTARHFIQSSLFYFSFGEVDFILD--------SSRGKNNLKFPQLLASQMSVAIRNLH 223
             ++ + ++  SL   S G  D+I +        SS   +   F  LL S  +  +  L+
Sbjct: 169 RKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELY 228

Query: 224 EAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVML 283
             G +K V  G+ PLGC P  L+    + AA+  E  CV+ +NE+   +N  +   +  L
Sbjct: 229 GKGFRKFVIAGVGPLGCIPDQLA----AQAALPGE--CVEAVNEMAELFNNRLVSLVDRL 288

Query: 284 ---NEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACG 343
              N+   +A  V+ + Y   ++I+ NP  YGFE +   CCG+G     I C+   + C 
Sbjct: 289 NSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCA 348

Query: 344 RVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDL 362
              RHV+WD ++PT A N ++A  A+      S C P  +  L
Sbjct: 349 FRDRHVFWDAFHPTQAFNLIIALRAFNGS--KSDCYPINLSQL 382

BLAST of CmoCh02G012150 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.2e-25
Identity = 95/348 (27.30%), Postives = 153/348 (43.97%), Query Frame = 0

Query: 17  KSLFFTAYVTLIGLLGLSLAAQ-GSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD 76
           KSLF     T++ L+ +S        I A+ + GDSSVD G N     +   NF     D
Sbjct: 5   KSLF-----TILFLIAMSSTVTFAGKIPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRD 64

Query: 77  SDG---------TTLLPHLLAEEMGVPHSQPWY-NQNGSIEAILNGLSFGSPQA---TIV 136
             G           +    ++E +G+    P Y + + +I     G++F S         
Sbjct: 65  FVGGKPTGRFCNGKIATDFMSEALGLKPIIPAYLDPSYNISDFATGVTFASAATGYDNAT 124

Query: 137 SGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILD------- 196
           S   S   L +QL    +    L+   G D     I+SSL+  S G  DF+ +       
Sbjct: 125 SDVLSVLPLWKQLEYYKEYQTKLKAYQGKDRGTETIESSLYLISIGTNDFLENYFAFPGR 184

Query: 197 SSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYD 256
           SS+   +L +   LA      ++ LH  GA+KI   G+ P+GC P       +    +  
Sbjct: 185 SSQYSVSL-YQDFLAGIAKEFVKKLHGLGARKISLGGLPPMGCMP------LERATNIGT 244

Query: 257 EKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESR 316
              CV   N++ V +N  ++K +  L+++L  + +VF + Y+  M II NP  +GFE   
Sbjct: 245 GGECVGRYNDIAVQFNSKLDKMVEKLSKELPGSNLVFSNPYEPFMRIIKNPSSFGFEVVG 304

Query: 317 SACCGLGSYNASIGCVAKE-IACGRVRRHVWWDLYNPTGAVNSLLANS 343
           +ACC  G +    GC       C    ++V+WD ++PT   N ++AN+
Sbjct: 305 AACCATGMFEMGYGCQRNNPFTCTNADKYVFWDSFHPTQKTNHIMANA 340

BLAST of CmoCh02G012150 vs. ExPASy TrEMBL
Match: A0A6J1H1Q6 (GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 7.8e-208
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL
Sbjct: 15  MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 74

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 75  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 134

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 135 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 194

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV
Sbjct: 195 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 254

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG
Sbjct: 255 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 314

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD
Sbjct: 315 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 374

Query: 361 LVK 364
           LVK
Sbjct: 375 LVK 377

BLAST of CmoCh02G012150 vs. ExPASy TrEMBL
Match: A0A6J1K3Z0 (GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490967 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.0e-200
Identity = 351/363 (96.69%), Postives = 357/363 (98.35%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMEL R AALFKSLFFTAYVTLIGLLGLSLAA+GSSITAMYLLGDSSVDCGLNAL
Sbjct: 15  MTLGVAMEL-RRAALFKSLFFTAYVTLIGLLGLSLAARGSSITAMYLLGDSSVDCGLNAL 74

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSI PCDSDGTTLLPHLLAE+MGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 75  FYPLLHRNFSITPCDSDGTTLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 134

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 135 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 194

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NN+KF QLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDE+GCV
Sbjct: 195 NNVKFAQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEQGCV 254

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLN QLGDARMVFCDAYKGMMEII NPRRYGFEESRSACCG
Sbjct: 255 KEMNELVVNYNELMEKQIVMLNAQLGDARMVFCDAYKGMMEIISNPRRYGFEESRSACCG 314

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRD+PFS+FCRPSTIGD
Sbjct: 315 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDRPFSNFCRPSTIGD 374

Query: 361 LVK 364
           LVK
Sbjct: 375 LVK 376

BLAST of CmoCh02G012150 vs. ExPASy TrEMBL
Match: A0A6J1H303 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 326/327 (99.69%), Postives = 327/327 (100.00%), Query Frame = 0

Query: 37  AQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQ 96
           +QGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQ
Sbjct: 2   SQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQ 61

Query: 97  PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH 156
           PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH
Sbjct: 62  PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH 121

Query: 157 FIQSSLFYFSFGEVDFILDSSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPL 216
           FIQSSLFYFSFGEVDFILDSSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPL
Sbjct: 122 FIQSSLFYFSFGEVDFILDSSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPL 181

Query: 217 GCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAY 276
           GCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAY
Sbjct: 182 GCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAY 241

Query: 277 KGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN 336
           KGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN
Sbjct: 242 KGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN 301

Query: 337 SLLANSAWRDQPFSSFCRPSTIGDLVK 364
           SLLANSAWRDQPFSSFCRPSTIGDLVK
Sbjct: 302 SLLANSAWRDQPFSSFCRPSTIGDLVK 328

BLAST of CmoCh02G012150 vs. ExPASy TrEMBL
Match: A0A6J1K1V7 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490967 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.6e-184
Identity = 316/327 (96.64%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 37  AQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQ 96
           ++GSSITAMYLLGDSSVDCGLNALFYPLLHRNFSI PCDSDGTTLLPHLLAE+MGVPHSQ
Sbjct: 2   SRGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSITPCDSDGTTLLPHLLAEKMGVPHSQ 61

Query: 97  PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH 156
           PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH
Sbjct: 62  PWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARH 121

Query: 157 FIQSSLFYFSFGEVDFILDSSRGKNNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPL 216
           FIQSSLFYFSFGEVDFILDSSRGKNN+KF QLLASQMSVAIRNLHEAGAKKIVCMGILPL
Sbjct: 122 FIQSSLFYFSFGEVDFILDSSRGKNNVKFAQLLASQMSVAIRNLHEAGAKKIVCMGILPL 181

Query: 217 GCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAY 276
           GCTPRVLSQWRDSPAAVYDE+GCVKEMNELVVNYNELMEKQIVMLN QLGDARMVFCDAY
Sbjct: 182 GCTPRVLSQWRDSPAAVYDEQGCVKEMNELVVNYNELMEKQIVMLNAQLGDARMVFCDAY 241

Query: 277 KGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN 336
           KGMMEII NPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN
Sbjct: 242 KGMMEIISNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVN 301

Query: 337 SLLANSAWRDQPFSSFCRPSTIGDLVK 364
           SLLANSAWRD+PFS+FCRPSTIGDLVK
Sbjct: 302 SLLANSAWRDRPFSNFCRPSTIGDLVK 328

BLAST of CmoCh02G012150 vs. ExPASy TrEMBL
Match: A0A5A7TDD0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00690 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 7.9e-136
Identity = 241/351 (68.66%), Postives = 289/351 (82.34%), Query Frame = 0

Query: 19  LFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDG 78
           L F A++ L+   G   AA  S++TAM+LLGDSSVDCG N LFYP LHRNFS++PCD++ 
Sbjct: 3   LIFIAHLILLINTG---AALESNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA 62

Query: 79  T-TLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQ 138
           T +LLP LLA+++G+PH+QPWYNQNGS+EAILNGL+FGSPQATI+S GQS+QSLNQQLRQ
Sbjct: 63  TSSLLPFLLADKIGLPHAQPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQ 122

Query: 139 VLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI-----LDSSRGKNNLKFPQLLASQ 198
           VLD +QLLRLQLG   ARHFIQSSLFY SFGEVDFI       S+R     +F +LL SQ
Sbjct: 123 VLDAIQLLRLQLGQHAARHFIQSSLFYLSFGEVDFINLYLLKSSARKYGGEEFARLLVSQ 182

Query: 199 MSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNE 258
           M +AIRNL EAGA+KIVCMGILPLGC+PRVLS+WRDSP   +D+KGCVKEMNEL+  YNE
Sbjct: 183 MVIAIRNLQEAGARKIVCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNE 242

Query: 259 LMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNAS-IGCV 318
           +M +++V LN +  D +M+FCD YKGM EIIGNP RYGF+ES+SACCG+G YNAS +GCV
Sbjct: 243 VMGEEMVKLNAEFEDTQMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCV 302

Query: 319 AKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 363
           A EIAC  VRR+VWWDLYNPTG VNSLLA+SAWR+QPFS+ C PST+ DL+
Sbjct: 303 AMEIACRDVRRYVWWDLYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

BLAST of CmoCh02G012150 vs. NCBI nr
Match: XP_022958402.1 (GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 732.6 bits (1890), Expect = 1.6e-207
Identity = 363/363 (100.00%), Postives = 363/363 (100.00%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL
Sbjct: 15  MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 74

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 75  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 134

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 135 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 194

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV
Sbjct: 195 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 254

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG
Sbjct: 255 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 314

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD
Sbjct: 315 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 374

Query: 361 LVK 364
           LVK
Sbjct: 375 LVK 377

BLAST of CmoCh02G012150 vs. NCBI nr
Match: KAG6605849.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 730.7 bits (1885), Expect = 6.1e-207
Identity = 362/363 (99.72%), Postives = 362/363 (99.72%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMELIR AALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL
Sbjct: 43  MTLGVAMELIRRAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 102

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 103 FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 162

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 163 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 222

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV
Sbjct: 223 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 282

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG
Sbjct: 283 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 342

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD
Sbjct: 343 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 402

Query: 361 LVK 364
           LVK
Sbjct: 403 LVK 405

BLAST of CmoCh02G012150 vs. NCBI nr
Match: KAG7035797.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 720.3 bits (1858), Expect = 8.3e-204
Identity = 356/357 (99.72%), Postives = 356/357 (99.72%), Query Frame = 0

Query: 7   MELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLH 66
           MELIR AALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLH
Sbjct: 1   MELIRRAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLH 60

Query: 67  RNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQ 126
           RNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQ
Sbjct: 61  RNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQ 120

Query: 127 SHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGKNNLKFP 186
           SHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGKNNLKFP
Sbjct: 121 SHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGKNNLKFP 180

Query: 187 QLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNEL 246
           QLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNEL
Sbjct: 181 QLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNEL 240

Query: 247 VVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNA 306
           VVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNA
Sbjct: 241 VVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNA 300

Query: 307 SIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLVK 364
           SIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLVK
Sbjct: 301 SIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLVK 357

BLAST of CmoCh02G012150 vs. NCBI nr
Match: XP_023533895.1 (GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.9 bits (1844), Expect = 3.5e-202
Identity = 356/363 (98.07%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMEL RSA LFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL
Sbjct: 15  MTLGVAMEL-RSAVLFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 74

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSIIPCDSDGT LLPHLLAE+MGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 75  FYPLLHRNFSIIPCDSDGTNLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 134

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 135 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 194

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV
Sbjct: 195 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 254

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG
Sbjct: 255 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 314

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRD+PFSSFCRPS I D
Sbjct: 315 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDRPFSSFCRPSAIED 374

Query: 361 LVK 364
           LVK
Sbjct: 375 LVK 376

BLAST of CmoCh02G012150 vs. NCBI nr
Match: XP_023533896.1 (GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.9 bits (1844), Expect = 3.5e-202
Identity = 356/363 (98.07%), Postives = 358/363 (98.62%), Query Frame = 0

Query: 1   MTLGVAMELIRSAALFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60
           MTLGVAMEL RSA LFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL
Sbjct: 1   MTLGVAMEL-RSAVLFKSLFFTAYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCGLNAL 60

Query: 61  FYPLLHRNFSIIPCDSDGTTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120
           FYPLLHRNFSIIPCDSDGT LLPHLLAE+MGVPHSQPWYNQNGSIEAILNGLSFGSPQAT
Sbjct: 61  FYPLLHRNFSIIPCDSDGTNLLPHLLAEKMGVPHSQPWYNQNGSIEAILNGLSFGSPQAT 120

Query: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180
           IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK
Sbjct: 121 IVSGGQSHQSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFILDSSRGK 180

Query: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240
           NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV
Sbjct: 181 NNLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCV 240

Query: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300
           KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG
Sbjct: 241 KEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCG 300

Query: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGD 360
           LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRD+PFSSFCRPS I D
Sbjct: 301 LGSYNASIGCVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDRPFSSFCRPSAIED 360

Query: 361 LVK 364
           LVK
Sbjct: 361 LVK 362

BLAST of CmoCh02G012150 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.0e-46
Identity = 101/335 (30.15%), Postives = 174/335 (51.94%), Query Frame = 0

Query: 42  ITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCDSDGTTLLP-------HLLAEEMGVPH 101
           + A+++ GDSSVD G N     L   +   +P   D  T  P        +  + +G+P 
Sbjct: 69  VPALFVFGDSSVDSGTNNFLGTLARAD--RLPYGRDFDTHQPTGRFCNGRIPVDYLGLPF 128

Query: 102 SQPWYNQNGSIEAILNGLSFGSPQATIV--SGGQSHQ--SLNQQLRQVLDTLQLLRLQLG 161
              +  Q G++E +  G+++ S  A I+  SG +  Q  S   Q+ Q +DT Q + L +G
Sbjct: 129 VPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRVSFAMQVEQFVDTFQQMILSIG 188

Query: 162 LDTARHFIQSSLFYFSFGEVDFILDSSRGKNNLK-------FPQLLASQMSVAIRNLHEA 221
              +   + +S+FY S G  D+I    R  +N++       F Q LAS M   ++ L+  
Sbjct: 189 EKASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTPWNFNQFLASNMRQELKTLYNV 248

Query: 222 GAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVMLNE 281
             +++V MG+ P+GC P  + ++R           C +E+N +++  N +M   +  LN 
Sbjct: 249 KVRRMVVMGLPPIGCAPYYMWKYRSQNGE------CAEEVNSMIMESNFVMRYTVDKLNR 308

Query: 282 QLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRVRRH 341
           +L  A +++CD ++  M+I+ N + YGF E+  ACCGLG Y   + C++ E+AC     H
Sbjct: 309 ELPGASIIYCDVFQSAMDILRNHQHYGFNETTDACCGLGRYKGWLPCISPEMACSDASGH 368

Query: 342 VWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTI 359
           +WWD ++PT AVN++LA++ W  +     C P+ +
Sbjct: 369 LWWDQFHPTDAVNAILADNVWNGR-HVDMCYPTNL 394

BLAST of CmoCh02G012150 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 5.1e-34
Identity = 99/332 (29.82%), Postives = 153/332 (46.08%), Query Frame = 0

Query: 42  ITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD---------SDGTTLLPHLLAEEMGV 101
           + AM++LGDS VD G N     +   NF     D         S+G T +  LLA  + +
Sbjct: 39  VPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNYQPTGRFSNGLTFI-DLLARLLEI 98

Query: 102 PHSQPWYNQNGSIEAILNGLSFGSPQATI--VSGGQ--SHQSLNQQLRQVLDTLQLLRLQ 161
           P   P+ +   S   IL G+++ S  A I  VSG       SLNQQ+  +  TL  LR  
Sbjct: 99  PSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTM 158

Query: 162 LGLDTARHFIQSSLFYFSFGEVDFI--------LDSSRGKNNLKFPQLLASQMSVAIRNL 221
           +       ++  SL    FG  D+I         DSS       F  LL SQ +  +  L
Sbjct: 159 MSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLLTL 218

Query: 222 HEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVM 281
           +  G +KI   G+ PLGC P   ++    P        CV  +N+++  +N+ ++  +  
Sbjct: 219 YSLGLRKIFIPGVAPLGCIPNQRARGISPP------DRCVDSVNQILGTFNQGLKSLVDQ 278

Query: 282 LNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGRV 341
           LN++   A  V+ + Y  + +I+ NP  YGF     ACCG+G     I C+  +  C   
Sbjct: 279 LNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCGIGRNQGQITCLPLQTPCPNR 338

Query: 342 RRHVWWDLYNPTGAVNSLLANSAWRDQPFSSF 353
            ++V+WD ++PT   NS+LA  A+   P  ++
Sbjct: 339 NQYVFWDAFHPTQTANSILARRAFYGPPSDAY 363

BLAST of CmoCh02G012150 vs. TAIR 10
Match: AT1G33811.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 4.2e-28
Identity = 90/341 (26.39%), Postives = 160/341 (46.92%), Query Frame = 0

Query: 33  LSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD-SDGTT-------LLPH 92
           +S   Q + +  +++ GDS VD G N     L   N+     D   GTT           
Sbjct: 23  VSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFPQGTTGRFTNGRTYVD 82

Query: 93  LLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATI--VSGGQ--SHQSLNQQLRQVLD 152
            LA+ +G  +  P Y++    +AIL G +F S  A I   +G    +H S+NQQ+     
Sbjct: 83  ALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNLGAHTSMNQQVELYTT 142

Query: 153 TLQLLRLQLGLDT--ARHFIQSSLFYFSFGEVDFI--------LDSSRGKNNLKFPQLLA 212
            +Q +      DT   + ++   +FY   G  D++          +S   N+  F + L 
Sbjct: 143 AVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYSTSTNYNDKTFAESLI 202

Query: 213 SQMSVAIRNLHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNY 272
              +  +  L++ GA+K++  G+  +GC P  L+++ +   +      C +++N  +V +
Sbjct: 203 KNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST---GRCNEKINNAIVVF 262

Query: 273 NELMEKQIVMLNE-QLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIG 332
           N  ++K +  LN+ QL  A+ V+ D+YK   ++  N   YGFE     CCG+G  N  I 
Sbjct: 263 NTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVVDKGCCGVGRNNGQIT 322

Query: 333 CVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFS 351
           C+  +  C    ++++WD ++PT   N LLA S +  + ++
Sbjct: 323 CLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSRAYT 359

BLAST of CmoCh02G012150 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.7e-27
Identity = 87/360 (24.17%), Postives = 170/360 (47.22%), Query Frame = 0

Query: 23  AYVTLIGLLGLSLAAQGSSITAMYLLGDSSVDCG-LNALFYPLLHRNFSIIPCDSDG--- 82
           A   +I     +  A  SS+   Y+ GDS  + G  N L Y L   +F     D  G   
Sbjct: 7   ALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDFSGGKA 66

Query: 83  ------TTLLPHLLAEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQ----SH 142
                    +  +++ ++G+    P+ + + + +A L+G+++ S  A I++         
Sbjct: 67  TGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGIYFIQR 126

Query: 143 QSLNQQLRQVLDTLQLLRLQLGLDTARHFIQSSLFYFSFGEVDFI-------LDSSRGKN 202
            + N Q+     T +++R ++G   A   +  ++++   G  D++       +   +   
Sbjct: 127 LTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMADGQQYT 186

Query: 203 NLKFPQLLASQMSVAIRNLHEAGAKKIVCMGILPLGCTP--RVLSQWRDSPAAVYDEKGC 262
           + +F +LL S +   +  +++ GA+K++  G+ PLGC P  RV S+ R           C
Sbjct: 187 HDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTR----------MC 246

Query: 263 VKEMNELVVNYNELMEKQIVMLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACC 322
           +  +NE V+ +N   +K ++ LN++L  A+  F D Y  ++++I NP  YGF+ + ++CC
Sbjct: 247 LNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIANTSCC 306

Query: 323 GLGSYNASIG--CVAKEIACGRVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPST 358
            +   + S+G  C+     C   +  V+WD ++P+ + N +LA     D  FSS    S+
Sbjct: 307 NV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILA-----DHLFSSLLSSSS 347

BLAST of CmoCh02G012150 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-27
Identity = 93/343 (27.11%), Postives = 154/343 (44.90%), Query Frame = 0

Query: 44  AMYLLGDSSVDCGLNALFYPLLHRNFSIIPCD----------SDGTTLLPHLLAEEMGVP 103
           AM++ GDS VD G N     L   N+     D          S+G T++   + E +G+P
Sbjct: 49  AMFVFGDSLVDNGNNNHLNSLARSNYLPYGIDFAGNQPTGRFSNGKTIV-DFIGELLGLP 108

Query: 104 HSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQ----SLNQQLRQVLDTLQLLRLQL 163
               + +       IL+G+++ S    I+     H     S+ +Q+     TL  +   +
Sbjct: 109 EIPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSM 168

Query: 164 GLDTARHFIQSSLFYFSFGEVDFILD--------SSRGKNNLKFPQLLASQMSVAIRNLH 223
             ++ + ++  SL   S G  D+I +        SS   +   F  LL S  +  +  L+
Sbjct: 169 RKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTTHLLELY 228

Query: 224 EAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIVML 283
             G +K V  G+ PLGC P  L+    + AA+  E  CV+ +NE+   +N  +   +  L
Sbjct: 229 GKGFRKFVIAGVGPLGCIPDQLA----AQAALPGE--CVEAVNEMAELFNNRLVSLVDRL 288

Query: 284 ---NEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACG 343
              N+   +A  V+ + Y   ++I+ NP  YGFE +   CCG+G     I C+   + C 
Sbjct: 289 NSDNKTASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCGVGRNRGEITCLPLAVPCA 348

Query: 344 RVRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDL 362
              RHV+WD ++PT A N ++A  A+      S C P  +  L
Sbjct: 349 FRDRHVFWDAFHPTQAFNLIIALRAFNGS--KSDCYPINLSQL 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVV17.1e-3329.82GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2... [more]
Q8L5Z15.9e-2726.39GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2... [more]
Q9FHQ13.8e-2624.17GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2... [more]
Q9FNP25.0e-2627.11GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2... [more]
Q9SJB44.2e-2527.30GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1H1Q67.8e-208100.00GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1K3Z06.0e-20096.69GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1H3031.6e-18999.69GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1K1V74.6e-18496.64GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5A7TDD07.9e-13668.66GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366... [more]
Match NameE-valueIdentityDescription
XP_022958402.11.6e-207100.00GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita moschata][more]
KAG6605849.16.1e-20799.72GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7035797.18.3e-20499.72GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023533895.13.5e-20298.07GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023533896.13.5e-20298.07GDSL esterase/lipase At1g71250-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G10950.12.0e-4630.15SGNH hydrolase-type esterase superfamily protein [more]
AT1G71250.15.1e-3429.82GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G33811.14.2e-2826.39GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G37690.12.7e-2724.17SGNH hydrolase-type esterase superfamily protein [more]
AT5G08460.13.5e-2727.11GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 128..148
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 35..362
NoneNo IPR availablePANTHERPTHR45648:SF7OS12G0126100 PROTEINcoord: 35..362
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 47..279
e-value: 7.6E-10
score: 39.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 33..347
e-value: 4.5E-48
score: 166.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G012150.1CmoCh02G012150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds