CmoCh02G011910 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G011910
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionL-ascorbate oxidase homolog
LocationCmo_Chr02: 7129972 .. 7131724 (-)
RNA-Seq ExpressionCmoCh02G011910
SyntenyCmoCh02G011910
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTCGTGTCCATATTTACCCTGCTGCTCTGCGTCTGCGCCGCATCCCTATGGGCTGTCCACGCCGAAGACCCTTACCTCTTCTTCAATTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTTGTCGTCAACGTCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTCCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAATTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATTCCAAGAGCCACACCACTTTGAAGCAATTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCGTCCTGATCAATGGCAAGAACGAAAAGGGCGATGGCAGTGATGAGCCACTCTTCACCATGAAGCCCGAGAAGACTTACAAGTACAGAATTTGCAACGTGGGGCTTAAGGCATCTCTTAATTTTAGAATCCAAAGCCATTCCATGAAGCTTGTTGAGATGGAAGGCTCACACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCATGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAATCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCTTGAAGAACATTCTTTCAACTAAAGCCATCATTCGTTACACCAATGGCAAAGGTCCTGCTTCTTCTGAACTTCCAGAGGCACCCATTGGGTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGACCCAACCCCCAAGGCTCCTTCCGCTATGGTTCAATCAACATTACTCGCACCATCAAGCTCGTCAATTCAGCCGGTAAGGTCGACGGTAAGTTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCATGAGAAGGTGCTCCAAGCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCGTAAGTATTATTAATACAATTGTTTCATTCCCCCAATGAGTTATAGCATATATGGGTAATGAATTGTTGTGTTGTTTAATGTGGTTTACAGCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAGCCAACTACAATCTTCTTGATGCGGTGAGCCGACACACCATCCAAGTTTATCCCAAGTCATGGGCGGCGATTCTTCTGACATTCGACAATTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGCGTGGTGAAGGGCATGCCGTTACCCAAGCCGTATACCATTTGA

mRNA sequence

ATGAGTTTCGTGTCCATATTTACCCTGCTGCTCTGCGTCTGCGCCGCATCCCTATGGGCTGTCCACGCCGAAGACCCTTACCTCTTCTTCAATTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTTGTCGTCAACGTCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTCCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAATTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATTCCAAGAGCCACACCACTTTGAAGCAATTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCGTCCTGATCAATGGCAAGAACGAAAAGGGCGATGGCAGTGATGAGCCACTCTTCACCATGAAGCCCGAGAAGACTTACAAGTACAGAATTTGCAACGTGGGGCTTAAGGCATCTCTTAATTTTAGAATCCAAAGCCATTCCATGAAGCTTGTTGAGATGGAAGGCTCACACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCATGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAATCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCTTGAAGAACATTCTTTCAACTAAAGCCATCATTCGTTACACCAATGGCAAAGGTCCTGCTTCTTCTGAACTTCCAGAGGCACCCATTGGGTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGACCCAACCCCCAAGGCTCCTTCCGCTATGGTTCAATCAACATTACTCGCACCATCAAGCTCGTCAATTCAGCCGGTAAGGTCGACGGTAAGTTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCATGAGAAGGTGCTCCAAGCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAGCCAACTACAATCTTCTTGATGCGGTGAGCCGACACACCATCCAAGTTTATCCCAAGTCATGGGCGGCGATTCTTCTGACATTCGACAATTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGCGTGGTGAAGGGCATGCCGTTACCCAAGCCGTATACCATTTGA

Coding sequence (CDS)

ATGAGTTTCGTGTCCATATTTACCCTGCTGCTCTGCGTCTGCGCCGCATCCCTATGGGCTGTCCACGCCGAAGACCCTTACCTCTTCTTCAATTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTTGTCGTCAACGTCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAACACAGGAAGAATTGCTGGCAAGACGGCCTCCCCGGGACCACCTGCCCCATCCCTGCTGGCACCAATTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGCGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATTCCAAGAGCCACACCACTTTGAAGCAATTCTTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCGTCCTGATCAATGGCAAGAACGAAAAGGGCGATGGCAGTGATGAGCCACTCTTCACCATGAAGCCCGAGAAGACTTACAAGTACAGAATTTGCAACGTGGGGCTTAAGGCATCTCTTAATTTTAGAATCCAAAGCCATTCCATGAAGCTTGTTGAGATGGAAGGCTCACACGTGGTCCAAAACGATTACCAATCCCTCGACGTCCATGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAATCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCTTGAAGAACATTCTTTCAACTAAAGCCATCATTCGTTACACCAATGGCAAAGGTCCTGCTTCTTCTGAACTTCCAGAGGCACCCATTGGGTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGTGCAGCTAGACCCAACCCCCAAGGCTCCTTCCGCTATGGTTCAATCAACATTACTCGCACCATCAAGCTCGTCAATTCAGCCGGTAAGGTCGACGGTAAGTTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCATGAGAAGGTGCTCCAAGCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAGCCAACTACAATCTTCTTGATGCGGTGAGCCGACACACCATCCAAGTTTATCCCAAGTCATGGGCGGCGATTCTTCTGACATTCGACAATTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGCGTGGTGAAGGGCATGCCGTTACCCAAGCCGTATACCATTTGA

Protein sequence

MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Homology
BLAST of CmoCh02G011910 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 8.6e-264
Identity = 413/552 (74.82%), Postives = 474/552 (85.87%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           M  V +    L + AA+   VHAEDPY    W VTYGT SPLGVPQQ ILING+FPGPNI
Sbjct: 1   MRGVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNI 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NST+NNNV++NVFN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQ
Sbjct: 61  NSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGS+FYYP+T MHRAAGG+GGLR+NSRLLIPVPYADPEDDYTVLIGDWY+KSHT LK+FL
Sbjct: 121 IGSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           D GRT+ RPDG++INGK+ KGDGSD PLFT+KP KTY+ RICNVG+K S+NFRIQ+H MK
Sbjct: 181 DGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHV+QNDY SLDVHVGQCF  +VTANQEP+DYYMVAS+RFLK +++T  ++RY 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYE 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
            GKGPASS+LP  P+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+
Sbjct: 301 GGKGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
            GKVDGKLR+A+NGVSH E ETPLKLAEYF + +KVFKYDTITD+  PE    I + PNV
Sbjct: 361 QGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LN T+R FVE++FENHEK +Q+WHL+GYSFF+VA+EPG W+PEKR NYNLLDAVSRHT+Q
Sbjct: 421 LNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPK WAAILLTFDNCGMWN+RSE TE RYLGQQLY SVLSP  SLRDEYN+P+ +L CG
Sbjct: 481 VYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

Query: 541 VVKGMPLP-KPY 552
           +VK  P P  PY
Sbjct: 541 LVKNTPKPVNPY 552

BLAST of CmoCh02G011910 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 2.4e-234
Identity = 374/552 (67.75%), Postives = 443/552 (80.25%), Query Frame = 0

Query: 4   VSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINST 63
           V+   LLLC+       V AEDPYL+FNW VTYGTI+PLGVPQQGILING+FPGP IN T
Sbjct: 6   VTFVALLLCLSV----GVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCT 65

Query: 64  TNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGS 123
           +NNN+VVNVFN+LDEPFL  W+G+QHRKN WQDG PGT CPI  G NFTY FQVKDQIGS
Sbjct: 66  SNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGS 125

Query: 124 FFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSG 183
           + Y+P+TA+HRAAGG+G L ++SR LIPVP+ +P D+Y V +GDWY+K H TLK+ LD G
Sbjct: 126 YSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGG 185

Query: 184 RTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVE 243
           RT+ RPDG++INGK+ K   + EPLFTM+  KTY+YR CN+G+++S+N R Q H MKLVE
Sbjct: 186 RTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVE 245

Query: 244 MEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGK 303
           +EGSH VQN Y SLD+HVGQC SVLVTA+QEP+DYY+V S+RFLK  LS+ AIIRY NGK
Sbjct: 246 LEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGK 305

Query: 304 GPASSELPEAP----IGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN 363
           GPAS ELP  P     G AWS+NQFR+FRWNLTASAARPNPQGS+ YG INITRTIK+ N
Sbjct: 306 GPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFN 365

Query: 364 SAGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPN 423
           S  +V GKLRY +NG+SH   ETPLKL EYF    K FKYD + DE  P   + +T+A N
Sbjct: 366 SMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEA-PADPSKLTIATN 425

Query: 424 VLNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTI 483
           V NATYRNFVEIIFENHEK ++ +HL+GYSFFAVA+EPG+WSPEKR NYNL+D +SR+ I
Sbjct: 426 VKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNI 485

Query: 484 QVYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLC 543
           QVYP SWAAI+LTFDN GMWNLRSE+ E  YLG+QLY SVLSP+ SLRDEYNIPD   LC
Sbjct: 486 QVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLC 545

Query: 544 GVVKGMPLPKPY 552
           G+VKG+ +P PY
Sbjct: 546 GIVKGLSMPAPY 552

BLAST of CmoCh02G011910 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.1e-154
Identity = 270/564 (47.87%), Postives = 369/564 (65.43%), Query Frame = 0

Query: 4   VSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINST 63
           + +F +LL V   ++    A DPY F+N++V+Y T SPLGVPQQ I ING+FPGP IN T
Sbjct: 1   MDLFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVT 60

Query: 64  TNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGS 123
           TN N+VVNV N LDE  L+HW+GIQ R+  WQDG+ GT CPIP   N+TY FQVKDQIGS
Sbjct: 61  TNENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGS 120

Query: 124 FFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSG 183
           FFY+PS    RA+GGFG   +N R +IPVP++ P+ D TV IGDWY ++HT L++ LD G
Sbjct: 121 FFYFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDG 180

Query: 184 RTLARPDGVLINGKNE-------KGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQS 243
           + L  PDGVLINGK           DG D    T+ P KTY+ R+ NVG+  SLNFRIQ 
Sbjct: 181 KDLGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQG 240

Query: 244 HSMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQE-PRDYYMVASTRFLKNILSTK- 303
           H++ L E EGS+ VQ +Y SLD+HVGQ +S LVT +Q    DYY+VAS R +   +  + 
Sbjct: 241 HNLVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRV 300

Query: 304 ---AIIRYTNGKGPASSELPEAP---IGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGS 363
               I++YTN KG A  +LP  P       +S+NQ R+ RWN++AS ARPNPQGSF+YGS
Sbjct: 301 TGVGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGS 360

Query: 364 INITRTIKLVNSAG-KVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGL 423
           IN+T    L N     + GK R  +NG+S     TP++LA+  KV + V+K D       
Sbjct: 361 INVTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLD------F 420

Query: 424 PEGSTT--ITVAPNVLNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKR 483
           P+   T    VA +++N TYR F+E++ +N++  +Q++H++GY+FF V ++ G+W+   R
Sbjct: 421 PKRPLTGPAKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSR 480

Query: 484 ANYNLLDAVSRHTIQVYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALS 543
             YN  D ++R TIQVYP +W+AIL++ DN G WNLR+E  ++ YLGQ+ YV V++P  +
Sbjct: 481 GTYNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDEN 540

Query: 544 LRDEYNIPDRTLLCGVVKGMPLPK 550
            + E+  PD  L CG +  +  P+
Sbjct: 541 NKTEFGHPDNVLYCGALSKLQKPQ 557

BLAST of CmoCh02G011910 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.3e-147
Identity = 261/551 (47.37%), Postives = 368/551 (66.79%), Query Frame = 0

Query: 13  VCAASLWAV-HAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNVVVN 72
           +C A L AV  A DP++ ++++V+Y T SPLGVPQQ I +NG+FPGP +N+TTN NVVVN
Sbjct: 13  LCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVVN 72

Query: 73  VFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYPSTA 132
           VFNHLDEP L+ W GIQ R+N WQDG+ GT CPIP   NFTY FQVKDQIGSFFY PS  
Sbjct: 73  VFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSLN 132

Query: 133 MHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRTLARPDG 192
             RA+GGFG + IN+R +IP+P+  P+ +   +IGDWY++ H  L++ LDSG+ L  PDG
Sbjct: 133 FQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDG 192

Query: 193 VLINGK------NEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 252
           VLINGK      +   DG D   F ++P KTY+ R+ NVG+  SLNFRIQ+HS+ LVE E
Sbjct: 193 VLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETE 252

Query: 253 GSHVVQNDYQSLDVHVGQCFSVLVTANQE-PRDYYMVASTRFLKNILSTK----AIIRYT 312
           G +  Q ++   DVHVGQ +S LVT +Q+   DYY+VAS RF+   +  +    AI+ Y+
Sbjct: 253 GHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILHYS 312

Query: 313 NGKGPASSEL--PEAPIGWAWS-LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKL 372
           N KGP S  L  P+  +   WS ++Q +T R N +AS ARPNPQGSF YG INIT T  L
Sbjct: 313 NSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTYIL 372

Query: 373 VNSAGK-VDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEG--STTI 432
            +     ++G LR  +NG+S V   TP++LA+  KV +  +K D       P+   +  +
Sbjct: 373 RSLPPTIINGALRATLNGISFVNPSTPVRLADRNKV-KGAYKLD------FPDRPFNRPL 432

Query: 433 TVAPNVLNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAV 492
            +  +++NATY+ F++++F+N++  +Q++H++GYSFF V ++ G WS +K+ +YN  DA+
Sbjct: 433 RLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAI 492

Query: 493 SRHTIQVYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPD 546
           SR TI+VYP  W A+L++ DN G+WN+R E  +  YLG++ Y+ + +P    + E + PD
Sbjct: 493 SRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPD 552

BLAST of CmoCh02G011910 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 3.0e-147
Identity = 254/540 (47.04%), Postives = 363/540 (67.22%), Query Frame = 0

Query: 23  AEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNVVVNVFNHLDEPFLI 82
           A DPY+ +++ ++Y T SPLGVPQQ I +NG+FPGP IN+TTN NV VNV NHLDEP L+
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 83  HWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGL 142
            W G+Q R+N WQDG+ GT CPIP   NFTY FQ+KDQIGS+FY PS    RA+GGFG L
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 143 RINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRTLARPDGVLINGK----- 202
            IN+R L+P+P+ +P+ +   +IGDWY+++HT L++ LDSG+ L  PDGVLINGK     
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 203 -NEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEMEGSHVVQNDYQS 262
            +   DG +     + P KTY+ R+ NVG+  SLNFRIQ+H + L+E EG +  Q ++  
Sbjct: 203 NSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTD 262

Query: 263 LDVHVGQCFSVLVTANQE-PRDYYMVASTRFLKNILSTK----AIIRYTNGKGPASSELP 322
            DVHVGQ +S LVT +Q    DYY+VAS RF+   +  +     I+ Y+N KGPAS  LP
Sbjct: 263 FDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLP 322

Query: 323 --EAPIGWAWS-LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN-SAGKVDGK 382
                +   WS +NQ R  + N +AS ARPNPQGSF YG INITRT  L +    K++GK
Sbjct: 323 VSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGK 382

Query: 383 LRYAINGVSHVETETPLKLAEYFKVP-EKVFKY-DTITDEGLPEGSTTITVAPNVLNATY 442
           LR  +NG+S V   TP++LA+  KV  + +  + D   DE LP  S++I      +NATY
Sbjct: 383 LRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSI------INATY 442

Query: 443 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 502
           + F+++IF+N++  +Q++H++GY+F+ VA++ G WS ++ ++YN  DAV+R T++VYP +
Sbjct: 443 KGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGA 502

Query: 503 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546
           W A+L++ DN G+WN+R E  +  YLGQ+ Y+ +++P  +   E + P+  + CG ++ M
Sbjct: 503 WTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of CmoCh02G011910 vs. ExPASy TrEMBL
Match: A0A6J1H2P0 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459150 PE=3 SV=1)

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI
Sbjct: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK
Sbjct: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
           NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS
Sbjct: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
           AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV
Sbjct: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ
Sbjct: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG
Sbjct: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540

Query: 541 VVKGMPLPKPYTI 554
           VVKGMPLPKPYTI
Sbjct: 541 VVKGMPLPKPYTI 553

BLAST of CmoCh02G011910 vs. ExPASy TrEMBL
Match: A0A6J1K1Q7 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=1)

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 537/553 (97.11%), Postives = 544/553 (98.37%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           MSFVSIFTLLLCVCAASLWAVHAEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNI
Sbjct: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNI 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NSTTNNNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHTTLKQFL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           DSGRTLARPDGVLINGKN KGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQ HSMK
Sbjct: 181 DSGRTLARPDGVLINGKNGKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQGHSMK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLK I  +KAIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKTIHMSKAIIRYT 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
           NGKGPASS+LPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS
Sbjct: 301 NGKGPASSDLPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
            GKVDGKLRYAINGVSHV+ ETPLKLAEYFKVPEKVFKYDTITDEG+PEGSTTITVAPNV
Sbjct: 361 VGKVDGKLRYAINGVSHVDPETPLKLAEYFKVPEKVFKYDTITDEGIPEGSTTITVAPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ
Sbjct: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPKSW A+LLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG
Sbjct: 481 VYPKSWTAVLLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540

Query: 541 VVKGMPLPKPYTI 554
           VVKGMPLPKPYTI
Sbjct: 541 VVKGMPLPKPYTI 553

BLAST of CmoCh02G011910 vs. ExPASy TrEMBL
Match: A0A6J1H0Y2 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 517/545 (94.86%), Postives = 529/545 (97.06%), Query Frame = 0

Query: 9   LLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV 68
           LLLC+ AASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV
Sbjct: 10  LLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV 69

Query: 69  VVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP 128
           VVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP
Sbjct: 70  VVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP 129

Query: 129 STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRTLAR 188
           STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHT LKQ LDSGRTLAR
Sbjct: 130 STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLAR 189

Query: 189 PDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEMEGSH 248
           PDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ+H MKLVEMEGSH
Sbjct: 190 PDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSH 249

Query: 249 VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGPASS 308
           VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KAIIRYTNGKGPASS
Sbjct: 250 VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASS 309

Query: 309 ELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVDGKL 368
           +LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VDGKL
Sbjct: 310 DLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKL 369

Query: 369 RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF 428
           RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF
Sbjct: 370 RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF 429

Query: 429 VEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKSWAA 488
           VEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKSWAA
Sbjct: 430 VEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAA 489

Query: 489 ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP 548
           ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP
Sbjct: 490 ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP 549

Query: 549 KPYTI 554
           KPYTI
Sbjct: 550 KPYTI 554

BLAST of CmoCh02G011910 vs. ExPASy TrEMBL
Match: A0A6J1K3U4 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 513/548 (93.61%), Postives = 528/548 (96.35%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           +F LLLC+  ASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTN
Sbjct: 7   LFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF
Sbjct: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHT LKQ LDSGRT
Sbjct: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           LARPDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ+H MKLVEME
Sbjct: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KAIIRYTNGKGP
Sbjct: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGP 306

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
           ASS+LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VD
Sbjct: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITVAPNVLNATY
Sbjct: 367 GKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDVITDEGIPEGSTTITVAPNVLNATY 426

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           RNFVEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKS
Sbjct: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545
           WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM
Sbjct: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546

Query: 546 PLPKPYTI 554
           PLPKPYTI
Sbjct: 547 PLPKPYTI 554

BLAST of CmoCh02G011910 vs. ExPASy TrEMBL
Match: A0A6J1K3U8 (L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490934 PE=3 SV=1)

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 513/558 (91.94%), Postives = 528/558 (94.62%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           +F LLLC+  ASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTN
Sbjct: 7   LFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF
Sbjct: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPE----DDYTVLIGDWYSKSHTTLKQFLD 185
           YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPE    DDYTVLIGDWY+KSHT LKQ LD
Sbjct: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTDDYTVLIGDWYTKSHTALKQLLD 186

Query: 186 SGRTLARPDGVLINGKNEK------GDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQ 245
           SGRTLARPDG+LINGKN K      GDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ
Sbjct: 187 SGRTLARPDGILINGKNAKGDGRFEGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQ 246

Query: 246 SHSMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKA 305
           +H MKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KA
Sbjct: 247 NHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKA 306

Query: 306 IIRYTNGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTI 365
           IIRYTNGKGPASS+LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTI
Sbjct: 307 IIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTI 366

Query: 366 KLVNSAGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTIT 425
           KLVNS G VDGKLRYAINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTIT
Sbjct: 367 KLVNSFGNVDGKLRYAINGVSHVEPETPLKLAEYFRVPEKVFKYDAITDEGIPEGSTTIT 426

Query: 426 VAPNVLNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVS 485
           VAPNVLNATYRNFVEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVS
Sbjct: 427 VAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVS 486

Query: 486 RHTIQVYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDR 545
           RHTIQV+PKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDR
Sbjct: 487 RHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDR 546

Query: 546 TLLCGVVKGMPLPKPYTI 554
           TLLCGVVKGMPLPKPYTI
Sbjct: 547 TLLCGVVKGMPLPKPYTI 564

BLAST of CmoCh02G011910 vs. NCBI nr
Match: XP_022957684.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI
Sbjct: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK
Sbjct: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
           NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS
Sbjct: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
           AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV
Sbjct: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ
Sbjct: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG
Sbjct: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540

Query: 541 VVKGMPLPKPYTI 554
           VVKGMPLPKPYTI
Sbjct: 541 VVKGMPLPKPYTI 553

BLAST of CmoCh02G011910 vs. NCBI nr
Match: XP_022995356.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 537/553 (97.11%), Postives = 544/553 (98.37%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           MSFVSIFTLLLCVCAASLWAVHAEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNI
Sbjct: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNI 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NSTTNNNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHTTLKQFL
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           DSGRTLARPDGVLINGKN KGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQ HSMK
Sbjct: 181 DSGRTLARPDGVLINGKNGKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQGHSMK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLK I  +KAIIRYT
Sbjct: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKTIHMSKAIIRYT 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
           NGKGPASS+LPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS
Sbjct: 301 NGKGPASSDLPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
            GKVDGKLRYAINGVSHV+ ETPLKLAEYFKVPEKVFKYDTITDEG+PEGSTTITVAPNV
Sbjct: 361 VGKVDGKLRYAINGVSHVDPETPLKLAEYFKVPEKVFKYDTITDEGIPEGSTTITVAPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ
Sbjct: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPKSW A+LLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG
Sbjct: 481 VYPKSWTAVLLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540

Query: 541 VVKGMPLPKPYTI 554
           VVKGMPLPKPYTI
Sbjct: 541 VVKGMPLPKPYTI 553

BLAST of CmoCh02G011910 vs. NCBI nr
Match: XP_022957683.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 517/545 (94.86%), Postives = 529/545 (97.06%), Query Frame = 0

Query: 9   LLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV 68
           LLLC+ AASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV
Sbjct: 10  LLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTNNNV 69

Query: 69  VVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP 128
           VVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP
Sbjct: 70  VVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFFYYP 129

Query: 129 STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRTLAR 188
           STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHT LKQ LDSGRTLAR
Sbjct: 130 STAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLAR 189

Query: 189 PDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEMEGSH 248
           PDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ+H MKLVEMEGSH
Sbjct: 190 PDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSH 249

Query: 249 VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGPASS 308
           VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KAIIRYTNGKGPASS
Sbjct: 250 VVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASS 309

Query: 309 ELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVDGKL 368
           +LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VDGKL
Sbjct: 310 DLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKL 369

Query: 369 RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF 428
           RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF
Sbjct: 370 RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNF 429

Query: 429 VEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKSWAA 488
           VEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKSWAA
Sbjct: 430 VEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAA 489

Query: 489 ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP 548
           ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP
Sbjct: 490 ILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP 549

Query: 549 KPYTI 554
           KPYTI
Sbjct: 550 KPYTI 554

BLAST of CmoCh02G011910 vs. NCBI nr
Match: KAG6605821.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6605822.1 L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 517/548 (94.34%), Postives = 530/548 (96.72%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           +F LLLC+ AASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTN
Sbjct: 7   LFALLLCLSAASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVVVN+FNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF
Sbjct: 67  NNVVVNIFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 126

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHT LKQ LDSGRT
Sbjct: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRT 186

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           LARPDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ+H MKLVEME
Sbjct: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KAIIRYTNGKGP
Sbjct: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGP 306

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
           ASS+LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VD
Sbjct: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYD ITDEGLPEGSTTITVAPNVLNATY
Sbjct: 367 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATY 426

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           RNFVEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKS
Sbjct: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545
           WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM
Sbjct: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546

Query: 546 PLPKPYTI 554
           PLPKPYTI
Sbjct: 547 PLPKPYTI 554

BLAST of CmoCh02G011910 vs. NCBI nr
Match: XP_023534711.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 513/548 (93.61%), Postives = 529/548 (96.53%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           +F LLLC+  ASLW V AEDPYLFF WKVTYGTISPLGVPQQGILINGEFPGPNINSTTN
Sbjct: 7   LFALLLCLSTASLWTVRAEDPYLFFTWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 66

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIP GTNFTYHFQVKDQIGSFF
Sbjct: 67  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPVGTNFTYHFQVKDQIGSFF 126

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWY+KSHT L+Q LDSGRT
Sbjct: 127 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALRQLLDSGRT 186

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           LARPDG+LINGKN KGDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ+H MKLVEME
Sbjct: 187 LARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEME 246

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRF+K I  +KA+IRYTNGKGP
Sbjct: 247 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAVIRYTNGKGP 306

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
           ASS+LPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VD
Sbjct: 307 ASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVD 366

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GKLRYAINGVSHVETETPLKLAEYF+VPEKVFKYDTITDEGLPEGS+TITVAPNVLNATY
Sbjct: 367 GKLRYAINGVSHVETETPLKLAEYFRVPEKVFKYDTITDEGLPEGSSTITVAPNVLNATY 426

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           RNFVEIIFENHEK LQ+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKS
Sbjct: 427 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 486

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545
           WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM
Sbjct: 487 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 546

Query: 546 PLPKPYTI 554
           PLPKPYTI
Sbjct: 547 PLPKPYTI 554

BLAST of CmoCh02G011910 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 916.0 bits (2366), Expect = 1.5e-266
Identity = 414/551 (75.14%), Postives = 477/551 (86.57%), Query Frame = 0

Query: 1   MSFVSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNI 60
           M  V +    L + AA+   V AEDPY    W VTYGT+SPLGVPQQ ILING+FPGPN+
Sbjct: 1   MRGVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNV 60

Query: 61  NSTTNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQ 120
           NST+NNNV++NVFN+LDEPFL+ W+GIQHRKNCWQDG PGT CPI  GTN+TYHFQ KDQ
Sbjct: 61  NSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFL 180
           IGS+FYYPSTAMHR+AGGFGGLR+NSRLLIPVPYADPEDDYTVLIGDWY+KSHT LK+FL
Sbjct: 121 IGSYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFL 180

Query: 181 DSGRTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMK 240
           DSGRTL RPDG+LINGK+ KGDGSD PLFT+KP KTY+ RICNVGLK SLNFRIQ+H +K
Sbjct: 181 DSGRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLK 240

Query: 241 LVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYT 300
           LVEMEGSHV+QNDY SLDVHVGQC+  ++TANQE +DYYMVAS+RFLK++++T  ++RY 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYE 300

Query: 301 NGKGPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS 360
            GKGPASS+LP  P+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+
Sbjct: 301 GGKGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNT 360

Query: 361 AGKVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNV 420
            GKVDGKLRYA+NGVSH + ETPLKLAEYF V +KVFKYD+ITD   PE   +I + PNV
Sbjct: 361 QGKVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNV 420

Query: 421 LNATYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQ 480
           LN T+R F+E++FENHEK +Q+WHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+Q
Sbjct: 421 LNITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQ 480

Query: 481 VYPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 540
           VYPK WAAILLTFDNCGMWN+RSE +E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG
Sbjct: 481 VYPKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

Query: 541 VVKGMPLPKPY 552
           +VKG P P PY
Sbjct: 541 LVKGTPKPNPY 551

BLAST of CmoCh02G011910 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 913.3 bits (2359), Expect = 9.4e-266
Identity = 415/548 (75.73%), Postives = 474/548 (86.50%), Query Frame = 0

Query: 4   VSIFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINST 63
           V +  + LCV  A++  V AEDPY    W VTYGT SPLGVPQQ ILING+FPGPNINST
Sbjct: 5   VKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINST 64

Query: 64  TNNNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGS 123
           +NNNV+VNVFN+LDEPFLI W+GIQHRKNCWQDG  GT CPIP G NFTYHFQ KDQIGS
Sbjct: 65  SNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGS 124

Query: 124 FFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSG 183
           +FYYP+TAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYT+LI DWY+KSHT LK+FLDSG
Sbjct: 125 YFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSG 184

Query: 184 RTLARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVE 243
           RT+ RPDG+LINGK+ K DGSD+PLFT+KP KTY+ RICNVGLKASLNFRIQ+H MKLVE
Sbjct: 185 RTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVE 244

Query: 244 MEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGK 303
           MEGSHV+QNDY SLDVHVGQCF V+VTA+QEP+DYYM+ASTRFLK  L+T  ++RY  GK
Sbjct: 245 MEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGK 304

Query: 304 GPASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGK 363
           GPASS+LP AP+GWAWSLNQ+R+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ GK
Sbjct: 305 GPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGK 364

Query: 364 VDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNA 423
           VDGKLRYA++GVSH + ETPLKLAEYF V +KVFKYDTI+D   P+    I + PNVLN 
Sbjct: 365 VDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNI 424

Query: 424 TYRNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYP 483
           T+R F+E++FENHE+ +Q+WHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QVYP
Sbjct: 425 THRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYP 484

Query: 484 KSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVK 543
           K WAAILLTFDNCGMWN+RSE  E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+VK
Sbjct: 485 KCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVK 544

Query: 544 GMPLPKPY 552
           G P   PY
Sbjct: 545 GKPKVNPY 552

BLAST of CmoCh02G011910 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 880.9 bits (2275), Expect = 5.2e-256
Identity = 407/547 (74.41%), Postives = 471/547 (86.11%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           + T+L+C+  +++  V+A DPY F  W VTYGT SPLGVPQ+ ILING+FPGPN+NST+N
Sbjct: 5   LLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSN 64

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVV+NVFNHLDEPFL+ WSGIQHRKNCWQDG+ GT+CPIPAG NFTYHFQ KDQIGS+F
Sbjct: 65  NNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYF 124

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           YYP+T++HR AGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWY+  HT LK FLDSGRT
Sbjct: 125 YYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSGRT 184

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           L  P+GVLINGK+ K  G +EPLFTMKP KTYKYR+CNVG K++LNFRIQ+H MKLVEME
Sbjct: 185 LGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEME 244

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           GSHV+QNDY SLDVHVGQCFSVLVTANQ  +DYYMVASTRFLK  LST  +IRY      
Sbjct: 245 GSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQ 304

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
           AS+ELP+AP+GWAWSLNQFR+FRWNLT++AARPNPQGS+ YG INITR+IKLVNS   VD
Sbjct: 305 ASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVD 364

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GK+R+  NGVSHV+TETPLKLAEYF++ EKVFKY+ I DE   +  T +TV PNVLN T+
Sbjct: 365 GKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDEPAAK-ITALTVQPNVLNITF 424

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           R FVEIIFENHEK +Q++HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QVYPKS
Sbjct: 425 RTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKS 484

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545
           W+AILLTFDN GMWN+RSE  E +YLG+QLYVSVLSP  SLRDEYNIP  T LCG+VKG+
Sbjct: 485 WSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGL 544

Query: 546 PLPKPYT 553
           PLP  Y+
Sbjct: 545 PLPAHYS 549

BLAST of CmoCh02G011910 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-253
Identity = 404/548 (73.72%), Postives = 471/548 (85.95%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           + T+L+C+ A+++  V A DPY ++ W VTYGT +PLG+PQQ ILING+FPGPN+NST+N
Sbjct: 6   LLTVLVCL-ASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSN 65

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           NNVV+NVFN+LDEPFL+ WSG+QHRKN WQDG+ GT+CPIPAGTNFTYHFQ KDQIGS+F
Sbjct: 66  NNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYF 125

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           YYPSTA+HR AGGFGGLR+NSRLLIPVPYADPEDD T+LI DWY+KSHT LK FLDSGRT
Sbjct: 126 YYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSGRT 185

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           L  PDGVLINGK+ K  G++ PLFTMKP KTYKYRICNVG K++LNFRIQ H MKLVEME
Sbjct: 186 LGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEME 245

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           GSHV+QNDY SLDVHVGQCF+VLVTA+Q  ++YYMVASTRFLK  +ST  ++ Y      
Sbjct: 246 GSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQ 305

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
           ASS++P+AP+GWAWSLNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKL N+   V+
Sbjct: 306 ASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVN 365

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GK+R+  NGVSHV+TETPLKLAEYF + EKVFKY+ I DE   +  TT+TV PNVLN T+
Sbjct: 366 GKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDEPAAK-ITTLTVEPNVLNITF 425

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           R FVE++FENHEK +Q++HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QVYPKS
Sbjct: 426 RTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKS 485

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 545
           W+AILLTFDN GMWN+RSE  E RYLGQQLYVSVLSP  SLRDEYNIP  T LCG+VKG+
Sbjct: 486 WSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGL 545

Query: 546 PLPKPYTI 554
           PLP PYTI
Sbjct: 546 PLPTPYTI 551

BLAST of CmoCh02G011910 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 641.0 bits (1652), Expect = 9.0e-184
Identity = 304/535 (56.82%), Postives = 387/535 (72.34%), Query Frame = 0

Query: 6   IFTLLLCVCAASLWAVHAEDPYLFFNWKVTYGTISPLGVPQQGILINGEFPGPNINSTTN 65
           I TLL  +  A      AEDPY FF W VTYG ISPLGV QQGILING+FPGP+I S TN
Sbjct: 12  ITTLLFLISLA-----FAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITN 71

Query: 66  NNVVVNVFNHLDEPFLIHWSGIQHRKNCWQDGLPGTTCPIPAGTNFTYHFQVKDQIGSFF 125
           +N+++NVFNHLDEPFL+ W+GI++ KN +QDG+ GT CPIP G N+TY  QVKDQIGSF+
Sbjct: 72  DNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFY 131

Query: 126 YYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYSKSHTTLKQFLDSGRT 185
           Y+PS   H+AAGGFGG+RI+SR LIPVP+  P DDYT+L+GDWY  +H  LK  LD+G  
Sbjct: 132 YFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGK 191

Query: 186 LARPDGVLINGKNEKGDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQSHSMKLVEME 245
           L  PDG+LING+      S      ++P KTY+ RI NVGL+ SLNFRIQ+H+MKLVE+E
Sbjct: 192 LPLPDGILINGR------SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVE 251

Query: 246 GSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFLKNILSTKAIIRYTNGKGP 305
           G + +QN + SLDVHVGQ +SVL+TA+Q  +DYY+V S+RF   IL+T  ++ Y+N   P
Sbjct: 252 GRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAP 311

Query: 306 ASSELPEAPIGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSAGKVD 365
            S  +P+ PI  +WS NQ R  R NLTAS  RPNPQGS+RYG INITRTI+L N+ G ++
Sbjct: 312 VSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIE 371

Query: 366 GKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATY 425
           GK RYA+N  S    +TPLKL +YFK+ + V+K  +I+D+     +  I    +V+ A +
Sbjct: 372 GKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ---PTNGAIFPTTSVMQADF 431

Query: 426 RNFVEIIFENHEKVLQAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKS 485
           R FVE+IFEN E ++Q+WHL+GYSF+ V +E GKWSP  R  YNL DA+ R TIQVYP+S
Sbjct: 432 RAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRS 491

Query: 486 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 541
           W AI +  DN GMWN+RSE+ E +YLGQQ Y+ V + + SLRDEY IP   LLCG
Sbjct: 492 WTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006248.6e-26474.82L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291622.4e-23467.75L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.1e-15447.87Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX52.3e-14747.37Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN63.0e-14747.04Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1H2P00.0e+00100.00L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459150 PE=3 S... [more]
A0A6J1K1Q70.0e+0097.11L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=... [more]
A0A6J1H0Y20.0e+0094.86L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 S... [more]
A0A6J1K3U40.0e+0093.61L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=... [more]
A0A6J1K3U80.0e+0091.94L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114909... [more]
Match NameE-valueIdentityDescription
XP_022957684.10.0e+00100.00L-ascorbate oxidase homolog [Cucurbita moschata][more]
XP_022995356.10.0e+0097.11L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_022957683.10.0e+0094.86L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxi... [more]
KAG6605821.10.0e+0094.34L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023534711.10.0e+0093.61L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascor... [more]
Match NameE-valueIdentityDescription
AT3G13390.11.5e-26675.14SKU5 similar 11 [more]
AT1G55570.19.4e-26675.73SKU5 similar 12 [more]
AT1G55560.15.2e-25674.41SKU5 similar 14 [more]
AT3G13400.11.1e-25373.72SKU5 similar 13 [more]
AT1G21860.19.0e-18456.82SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..300
e-value: 1.7E-39
score: 135.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..337
e-value: 5.8E-36
score: 126.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..544
e-value: 2.0E-33
score: 117.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..148
e-value: 5.1E-33
score: 115.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 349..539
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 156..338
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 21..174
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 34..147
e-value: 6.5E-40
score: 135.9
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 383..520
e-value: 5.0E-24
score: 84.6
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 5..544
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 5..544
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 28..145
e-value: 4.25297E-73
score: 226.52
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 159..299
e-value: 2.04132E-85
score: 259.257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G011910.1CmoCh02G011910.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity