CmoCh02G011890 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G011890
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionL-ascorbate oxidase homolog
LocationCmo_Chr02: 7123751 .. 7125978 (-)
RNA-Seq ExpressionCmoCh02G011890
SyntenyCmoCh02G011890
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTAGTTATGGAGGGACCGTTTCATTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGTGTCAGCCCCCATATATGGTCTTATGACTTTGCGGAGGCCTCCCGAAGTTCCAAGGCTATTTTGCCGAGTTTCTTAGATAATCAATAATAATTTATGTTAGTTAATAATTGTTTATTTAGAAAATTATGTTAATTAATAATTGTTTATTTAGAAAATTATGTTCATTAATAATTGTTTATTTAGAAAATTATGTTAATTAATAATTGTTTATTTAGAAAATTATGTTAATTAATAATTTTTTATTTAGAAAATTTATGTTAATTAATAATTCTTAGTTCGATACTTTTGTATCTTAAATTATGTTCATAATTTTCGATATTGTTCCCCAAAATTATAATGAATTAACATAATTTAAGATACAAAAGTATCGAACTAAGAATTATTAATTAACATAAATTTTCTAAATAAAANTGGACGGTCCGCGCCGAAGATCCTTACCTCTTCTTCACTTGGAAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAGCACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCCGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGTGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCCTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTGCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGCGCTGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCGTAAGTATCACTAATGCAATTGTTTCGTTGTAGCATATATGGGTAATGAATTGTTGTGTTGTTTAATGTGGTTTACAGCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

mRNA sequence

ATGACTAGTTATGGAGGGACCGTTTCATTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAGCACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCCGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGTGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCCTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTGCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGCGCTGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

Coding sequence (CDS)

ATGACTAGTTATGGAGGGACCGTTTCATTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGTCACCTACGGCACCATCTCTCCGCTTGGCGTCCCCCAGCAAGGCATCCTCATCAACGGCGAGTTCCCCGGCCCTAACATCAACTCCACCACCAACAACAACGTCGTTGTCAACATCTTCAACCACCTCGACGAGCCCTTCCTCATTCATTGGAGCGGCATCCAGCACAGGAAGAATTGCTGGCAAGACGGCCTTCCCGGGACCACCTGCCCCATCCCTGCCGGCACCAACTTCACCTACCATTTCCAAGTCAAGGACCAAATCGGAAGCTTCTTCTACTACCCTTCCACCGCCATGCACCGAGCCGCCGGTGGCTTCGGTGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGGGCCTCCGCATCAACAGCCGTCTACTCATCCCCGTCCCTTATGCCGACCCTGAAGACGACTACACCGTCCTCATTGGCGATTGGTATACCAAGAGCCACACCGCTCTGAAGCAACTCCTGGACAGCGGCCGCACCCTGGCTAGACCCGACGGCATCCTCATCAACGGCAAGAACGCAAAGGGCGATGGCAGTGACGAGCCACTCTTCACCATGAAGCCCGAGAAGACCTACAAGTACAGAATTTGCAATGTGGGGCTTAGGACGTCGCTTAACTTCAGAATCCAAAACCATCCCATGAAGCTTGTTGAGATGGAGGGCTCCCACGTGGTGCAAAACGATTACCAATCCCTCGACGTCCACGTGGGGCAATGCTTCTCTGTTTTGGTCACTGCCAACCAAGAGCCCAGAGACTATTACATGGTGGCATCCACTCGGTTCATGAAGACCATTCACATGAGCAAAGCCATTATTCGATACACCAATGGCAAAGGTCCTGCTTCTTCTGATCTTCCAGAGGCACCCATGGGTTGGGCTTGGTCCCTCAATCAATTCCGCACCTTCCGATGGAACCTCACTGCCAGCGCTGCTAGGCCTAACCCCCAAGGCTCCTTCCGCTATGGTTCCATCAACATTACTCGCACCATCAAGCTCGTCAATTCCTTCGGTAACGTCGACGGTAAGCTACGATATGCCATCAACGGCGTCTCTCATGTCGAAACCGAAACTCCATTGAAGCTTGCGGAATACTTCAAAGTCCCTGAGAAGGTGTTCAAGTACGACACGATTACCGACGAGGGATTACCAGAAGGTTCGACCACCATCACTGTGGCTCCTAATGTTCTCAATGCAACCTACCGCAACTTCGTTGAGATCATTTTTGAGAACCACGAGAAGAGGCTCCAATCATGGCATTTGAATGGCTACTCCTTCTTTGCCGTTGCCATCGAGCCAGGGAAATGGTCTCCAGAAAAGCGAACCAACTACAATCTTCTTGATGCGGTGAGCAGGCACACCATCCAAGTTTTCCCCAAGTCGTGGGCGGCCATTCTTCTGACATTCGACAACTGTGGAATGTGGAACTTGAGGTCTGAGCTGACAGAAAACCGTTACTTGGGACAGCAACTGTATGTCAGTGTCCTTTCCCCAGCTCTGTCGCTCCGAGATGAGTACAACATTCCAGACCGCACTTTGCTATGTGGAGTGGTGAAGGGCATGCCATTACCCAAACCATATACCATTTGA

Protein sequence

MTSYGGTVSFTNFLDLPALGRSPTAPSLRLASPSKASSSTASSPALTSTPPPTTTSLSTSSTTSTSPSSFIGAASSTGRIAGKTAFPGPPAPSLPAPTSPTISKSRTKSEASSTTLPPPCTEPPVASVASASTAVYSSPSLMPTLKTTTPGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Homology
BLAST of CmoCh02G011890 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 7.8e-196
Identity = 313/412 (75.97%), Postives = 357/412 (86.65%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LD GRT+ RPDGI+INGK+ K
Sbjct: 141 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSGK 200

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSD PLFT+KP KTY+ RICNVG++TS+NFRIQNH MKLVEMEGSHV+QNDY SLDVH
Sbjct: 201 GDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYDSLDVH 260

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCF  +VTANQEP+DYYMVAS+RF+KT+  +  ++RY  GKGPASS LP  P+GWAWS
Sbjct: 261 VGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQLPAGPVGWAWS 320

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ G VDGKLR+A+NGVSH E 
Sbjct: 321 LNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRFALNGVSHTEP 380

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF + +KVFKYDTITD+  PE    I + PNVLN T+R FVE++FENHEK +
Sbjct: 381 ETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTFVEVVFENHEKSV 440

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHL+GYSFF+VA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDNCGMWN
Sbjct: 441 QSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWN 500

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLP-KPY 562
           +RSE TE RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+VK  P P  PY
Sbjct: 501 VRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTPKPVNPY 552

BLAST of CmoCh02G011890 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.8e-169
Identity = 275/414 (66.43%), Postives = 329/414 (79.47%), Query Frame = 0

Query: 152 LRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAKG 211
           L ++SR LIPVP+ +P D+Y V +GDWY K H  LK++LD GRT+ RPDGI+INGK+AK 
Sbjct: 140 LNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGIIINGKSAKV 199

Query: 212 DGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVHV 271
             + EPLFTM+  KTY+YR CN+G+R+S+N R Q HPMKLVE+EGSH VQN Y SLD+HV
Sbjct: 200 GEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQNIYDSLDLHV 259

Query: 272 GQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAP----MGW 331
           GQC SVLVTA+QEP+DYY+V S+RF+K    S AIIRY NGKGPAS +LP  P     G 
Sbjct: 260 GQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPELPTPPPENTEGI 319

Query: 332 AWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSH 391
           AWS+NQFR+FRWNLTASAARPNPQGS+ YG INITRTIK+ NS   V GKLRY +NG+SH
Sbjct: 320 AWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKLRYGLNGISH 379

Query: 392 VETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHE 451
              ETPLKL EYF    K FKYD + DE  P   + +T+A NV NATYRNFVEIIFENHE
Sbjct: 380 TNGETPLKLVEYFGATNKAFKYDLMADEA-PADPSKLTIATNVKNATYRNFVEIIFENHE 439

Query: 452 KRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCG 511
           K ++++HL+GYSFFAVA+EPG+WSPEKR NYNL+D +SR+ IQV+P SWAAI+LTFDN G
Sbjct: 440 KTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAIMLTFDNAG 499

Query: 512 MWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPY 562
           MWNLRSE+ E  YLG+QLY SVLSP+ SLRDEYNIPD   LCG+VKG+ +P PY
Sbjct: 500 MWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKGLSMPAPY 552

BLAST of CmoCh02G011890 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.0e-102
Identity = 188/424 (44.34%), Postives = 268/424 (63.21%), Query Frame = 0

Query: 154 INSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGK------ 213
           +N R +IPVP++ P+ D TV IGDWY ++HTAL++ LD G+ L  PDG+LINGK      
Sbjct: 141 VNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLINGKGPYRYN 200

Query: 214 -NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQS 273
                DG D    T+ P KTY+ R+ NVG+ TSLNFRIQ H + L E EGS+ VQ +Y S
Sbjct: 201 DTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSYTVQQNYTS 260

Query: 274 LDVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHMSK----AIIRYTNGKGPASSDLP 333
           LD+HVGQ +S LVT +Q    DYY+VAS R +      +     I++YTN KG A   LP
Sbjct: 261 LDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSKGKAKGQLP 320

Query: 334 EAP---MGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFG-NVDGK 393
             P       +S+NQ R+ RWN++AS ARPNPQGSF+YGSIN+T    L N     + GK
Sbjct: 321 PGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNMPPVTISGK 380

Query: 394 LRYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTT--ITVAPNVLNATY 453
            R  +NG+S     TP++LA+  KV + V+K D       P+   T    VA +++N TY
Sbjct: 381 RRTTLNGISFKNPSTPIRLADKLKVKD-VYKLD------FPKRPLTGPAKVATSIINGTY 440

Query: 454 RNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKS 513
           R F+E++ +N++ ++QS+H++GY+FF V ++ G+W+   R  YN  D ++R TIQV+P +
Sbjct: 441 RGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYPGA 500

Query: 514 WAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 560
           W+AIL++ DN G WNLR+E  ++ YLGQ+ YV V++P  + + E+  PD  L CG +  +
Sbjct: 501 WSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCGALSKL 557

BLAST of CmoCh02G011890 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.5e-98
Identity = 181/421 (42.99%), Postives = 275/421 (65.32%), Query Frame = 0

Query: 152 LRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGK---- 211
           L IN+R L+P+P+ +P+ +   +IGDWYT++HTAL+++LDSG+ L  PDG+LINGK    
Sbjct: 142 LIINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFK 201

Query: 212 --NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQ 271
             ++  DG +     + P KTY+ R+ NVG+ TSLNFRIQNH + L+E EG +  Q ++ 
Sbjct: 202 YNSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFT 261

Query: 272 SLDVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHMSK----AIIRYTNGKGPASSDL 331
             DVHVGQ +S LVT +Q    DYY+VAS RF+      +     I+ Y+N KGPAS  L
Sbjct: 262 DFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPL 321

Query: 332 P--EAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVN-SFGNVDG 391
           P     +   WS +NQ R  + N +AS ARPNPQGSF YG INITRT  L +     ++G
Sbjct: 322 PVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKING 381

Query: 392 KLRYAINGVSHVETETPLKLAEYFKVP-EKVFKY-DTITDEGLPEGSTTITVAPNVLNAT 451
           KLR  +NG+S V   TP++LA+  KV  + +  + D   DE LP  S++I      +NAT
Sbjct: 382 KLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSI------INAT 441

Query: 452 YRNFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPK 511
           Y+ F+++IF+N++ ++QS+H++GY+F+ VA++ G WS ++ ++YN  DAV+R T++V+P 
Sbjct: 442 YKGFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPG 501

Query: 512 SWAAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKG 556
           +W A+L++ DN G+WN+R E  +  YLGQ+ Y+ +++P  +   E + P+  + CG ++ 
Sbjct: 502 AWTAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQA 556

BLAST of CmoCh02G011890 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 9.2e-96
Identity = 179/419 (42.72%), Postives = 269/419 (64.20%), Query Frame = 0

Query: 154 INSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGK------ 213
           IN+R +IP+P+  P+ +   +IGDWYT+ H AL++ LDSG+ L  PDG+LINGK      
Sbjct: 145 INNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPDGVLINGKGPYKYN 204

Query: 214 NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSL 273
           ++  DG D   F ++P KTY+ R+ NVG+ TSLNFRIQNH + LVE EG +  Q ++   
Sbjct: 205 SSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVETEGHYTSQANFTDF 264

Query: 274 DVHVGQCFSVLVTANQE-PRDYYMVASTRFMKTIHMSK----AIIRYTNGKGPASSDL-- 333
           DVHVGQ +S LVT +Q+   DYY+VAS RF+      +    AI+ Y+N KGP S  L  
Sbjct: 265 DVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILHYSNSKGPVSGPLPV 324

Query: 334 PEAPMGWAWS-LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGN-VDGKL 393
           P+  +   WS ++Q +T R N +AS ARPNPQGSF YG INIT T  L +     ++G L
Sbjct: 325 PKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTYILRSLPPTIINGAL 384

Query: 394 RYAINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEG--STTITVAPNVLNATYR 453
           R  +NG+S V   TP++LA+  KV +  +K D       P+   +  + +  +++NATY+
Sbjct: 385 RATLNGISFVNPSTPVRLADRNKV-KGAYKLD------FPDRPFNRPLRLDRSMINATYK 444

Query: 454 NFVEIIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSW 513
            F++++F+N++ ++QS+H++GYSFF V ++ G WS +K+ +YN  DA+SR TI+V+P  W
Sbjct: 445 GFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTIEVYPGGW 504

Query: 514 AAILLTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGM 556
            A+L++ DN G+WN+R E  +  YLG++ Y+ + +P    + E + PD  L CG +K +
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYCGALKNL 556

BLAST of CmoCh02G011890 vs. ExPASy TrEMBL
Match: A0A6J1H0Y2 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.9e-246
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. ExPASy TrEMBL
Match: A0A6J1K3U4 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 2.1e-244
Identity = 409/413 (99.03%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVE 
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVEP 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFRVPEKVFKYDVITDEGIPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. ExPASy TrEMBL
Match: A0A6J1K3U8 (L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490934 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 1.1e-240
Identity = 409/423 (96.69%), Postives = 411/423 (97.16%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPE----DDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILING 210
           GLRINSRLLIPVPYADPE    DDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILING
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILING 201

Query: 211 KNAK------GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVV 270
           KNAK      GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVV
Sbjct: 202 KNAKGDGRFEGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVV 261

Query: 271 QNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDL 330
           QNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDL
Sbjct: 262 QNDYQSLDVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDL 321

Query: 331 PEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRY 390
           PEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRY
Sbjct: 322 PEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRY 381

Query: 391 AINGVSHVETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVE 450
           AINGVSHVE ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITVAPNVLNATYRNFVE
Sbjct: 382 AINGVSHVEPETPLKLAEYFRVPEKVFKYDAITDEGIPEGSTTITVAPNVLNATYRNFVE 441

Query: 451 IIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAIL 510
           IIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAIL
Sbjct: 442 IIFENHEKRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAIL 501

Query: 511 LTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKP 564
           LTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKP
Sbjct: 502 LTFDNCGMWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKP 561

BLAST of CmoCh02G011890 vs. ExPASy TrEMBL
Match: A0A6J1K1Q7 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 8.0e-236
Identity = 392/413 (94.92%), Postives = 401/413 (97.09%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHT LKQ LDSGRTLARPDG+LINGKN K
Sbjct: 141 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQFLDSGRTLARPDGVLINGKNGK 200

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGL+ SLNFRIQ H MKLVEMEGSHVVQNDYQSLDVH
Sbjct: 201 GDGSDEPLFTMKPEKTYKYRICNVGLKASLNFRIQGHSMKLVEMEGSHVVQNDYQSLDVH 260

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRF+KTIHMSKAIIRYTNGKGPASSDLPEAP+GWAWS
Sbjct: 261 VGQCFSVLVTANQEPRDYYMVASTRFLKTIHMSKAIIRYTNGKGPASSDLPEAPIGWAWS 320

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNS G VDGKLRYAINGVSHV+ 
Sbjct: 321 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSVGKVDGKLRYAINGVSHVDP 380

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYFKVPEKVFKYDTITDEG+PEGSTTITVAPNVLNATYRNFVEIIFENHEK L
Sbjct: 381 ETPLKLAEYFKVPEKVFKYDTITDEGIPEGSTTITVAPNVLNATYRNFVEIIFENHEKVL 440

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           Q+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKSW A+LLTFDNCGMWN
Sbjct: 441 QAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKSWTAVLLTFDNCGMWN 500

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 501 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 553

BLAST of CmoCh02G011890 vs. ExPASy TrEMBL
Match: A0A6J1K1R2 (L-ascorbate oxidase homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490934 PE=3 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 1.0e-235
Identity = 400/416 (96.15%), Postives = 405/416 (97.36%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTK---SHTALKQLLDSGRTLARPDGILINGK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTK   SHTALKQLLDSGRTLARPDGILINGK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTSHTALKQLLDSGRTLARPDGILINGK 201

Query: 211 NAKGDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSL 270
           NAKGDG+      ++PEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSL
Sbjct: 202 NAKGDGN------LQPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSL 261

Query: 271 DVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGW 330
           DVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGW
Sbjct: 262 DVHVGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGW 321

Query: 331 AWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSH 390
           AWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSH
Sbjct: 322 AWSLNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSH 381

Query: 391 VETETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHE 450
           VE ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITVAPNVLNATYRNFVEIIFENHE
Sbjct: 382 VEPETPLKLAEYFRVPEKVFKYDAITDEGIPEGSTTITVAPNVLNATYRNFVEIIFENHE 441

Query: 451 KRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCG 510
           KRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCG
Sbjct: 442 KRLQSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCG 501

Query: 511 MWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           MWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 MWNLRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 551

BLAST of CmoCh02G011890 vs. NCBI nr
Match: XP_022957683.1 (L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 860.9 bits (2223), Expect = 6.1e-246
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. NCBI nr
Match: KAG6605821.1 (L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG6605822.1 L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.6 bits (2217), Expect = 3.0e-245
Identity = 412/413 (99.76%), Postives = 412/413 (99.76%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYFKVPEKVFKYD ITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFKVPEKVFKYDMITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. NCBI nr
Match: XP_023534711.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 856.7 bits (2212), Expect = 1.1e-244
Identity = 409/413 (99.03%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTAL+QLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALRQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKA+IRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAVIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF+VPEKVFKYDTITDEGLPEGS+TITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFRVPEKVFKYDTITDEGLPEGSSTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. NCBI nr
Match: XP_022995355.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 854.7 bits (2207), Expect = 4.4e-244
Identity = 409/413 (99.03%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK
Sbjct: 142 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 202 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 262 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVE 
Sbjct: 322 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVEP 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF+VPEKVFKYD ITDEG+PEGSTTITVAPNVLNATYRNFVEIIFENHEKRL
Sbjct: 382 ETPLKLAEYFRVPEKVFKYDVITDEGIPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN
Sbjct: 442 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 502 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 554

BLAST of CmoCh02G011890 vs. NCBI nr
Match: XP_023533003.1 (L-ascorbate oxidase homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848.6 bits (2191), Expect = 3.1e-242
Identity = 404/413 (97.82%), Postives = 411/413 (99.52%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTAL+QLLDSGRTLARPDGILINGKNAK
Sbjct: 69  GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALRQLLDSGRTLARPDGILINGKNAK 128

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH
Sbjct: 129 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 188

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKA+IRYTNGKGPASSDLPEAPMGWAWS
Sbjct: 189 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAVIRYTNGKGPASSDLPEAPMGWAWS 248

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET
Sbjct: 249 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 308

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF+VPEKVFKYDTITDEGLPEGS+TITVAPNVLNATYRNFVEIIFENHEK L
Sbjct: 309 ETPLKLAEYFRVPEKVFKYDTITDEGLPEGSSTITVAPNVLNATYRNFVEIIFENHEKVL 368

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           Q+WHLNGYSFFAVAIEPGKWSPEKR NYNLLDAVSRHTIQV+PKSWAAILLTFDNCGMWN
Sbjct: 369 QAWHLNGYSFFAVAIEPGKWSPEKRANYNLLDAVSRHTIQVYPKSWAAILLTFDNCGMWN 428

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           LRSELTENRYLGQQ+YVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI
Sbjct: 429 LRSELTENRYLGQQMYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 481

BLAST of CmoCh02G011890 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 687.6 bits (1773), Expect = 8.6e-198
Identity = 313/411 (76.16%), Postives = 358/411 (87.10%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHT LK+ LDSGRTL RPDGILINGK+ K
Sbjct: 141 GLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDSGRTLGRPDGILINGKSGK 200

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
           GDGSD PLFT+KP KTY+ RICNVGL+TSLNFRIQNH +KLVEMEGSHV+QNDY SLDVH
Sbjct: 201 GDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEMEGSHVLQNDYDSLDVH 260

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQC+  ++TANQE +DYYMVAS+RF+K++  +  ++RY  GKGPASS LP  P+GWAWS
Sbjct: 261 VGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGPASSQLPPGPVGWAWS 320

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ G VDGKLRYA+NGVSH + 
Sbjct: 321 LNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRYALNGVSHTDP 380

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF V +KVFKYD+ITD   PE   +I + PNVLN T+R F+E++FENHEK +
Sbjct: 381 ETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITHRTFIEVVFENHEKSV 440

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDNCGMWN
Sbjct: 441 QSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWN 500

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPY 562
           +RSE +E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+VKG P P PY
Sbjct: 501 VRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGTPKPNPY 551

BLAST of CmoCh02G011890 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 675.2 bits (1741), Expect = 4.4e-194
Identity = 308/411 (74.94%), Postives = 354/411 (86.13%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLR+NSRLLIPVPYADPEDDYT+LI DWYTKSHT LK+ LDSGRT+ RPDGILINGK+ K
Sbjct: 142 GLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSGRTIGRPDGILINGKSGK 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
            DGSD+PLFT+KP KTY+ RICNVGL+ SLNFRIQNH MKLVEMEGSHV+QNDY SLDVH
Sbjct: 202 TDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEMEGSHVLQNDYDSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCF V+VTA+QEP+DYYM+ASTRF+K    +  ++RY  GKGPASS LP AP+GWAWS
Sbjct: 262 VGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGPASSQLPAAPVGWAWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQ+R+FRWNLTASAARPNPQGS+ YG INITRTIKLVN+ G VDGKLRYA++GVSH + 
Sbjct: 322 LNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKLRYALSGVSHTDP 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF V +KVFKYDTI+D   P+    I + PNVLN T+R F+E++FENHE+ +
Sbjct: 382 ETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITHRTFIEVVFENHERSV 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHL+GYSFFAVA+EPG W+PEKR NYNLLDAVSRHT+QV+PK WAAILLTFDNCGMWN
Sbjct: 442 QSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAAILLTFDNCGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPY 562
           +RSE  E RYLGQQLY SVLSP  SLRDEYN+P+ +L CG+VKG P   PY
Sbjct: 502 IRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGKPKVNPY 552

BLAST of CmoCh02G011890 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 649.4 bits (1674), Expect = 2.6e-186
Identity = 304/412 (73.79%), Postives = 351/412 (85.19%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLR+NSRLLIPVPYADPEDDYTVL+GDWYT  HTALK  LDSGRTL  P+G+LINGK+ K
Sbjct: 139 GLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSGRTLGLPNGVLINGKSGK 198

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
             G +EPLFTMKP KTYKYR+CNVG +++LNFRIQNH MKLVEMEGSHV+QNDY SLDVH
Sbjct: 199 VGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEMEGSHVIQNDYDSLDVH 258

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCFSVLVTANQ  +DYYMVASTRF+K    +  +IRY      AS++LP+AP+GWAWS
Sbjct: 259 VGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQASTELPKAPVGWAWS 318

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFR+FRWNLT++AARPNPQGS+ YG INITR+IKLVNS   VDGK+R+  NGVSHV+T
Sbjct: 319 LNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVDGKVRFGFNGVSHVDT 378

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF++ EKVFKY+ I DE   +  T +TV PNVLN T+R FVEIIFENHEK +
Sbjct: 379 ETPLKLAEYFQMSEKVFKYNVIKDEPAAK-ITALTVQPNVLNITFRTFVEIIFENHEKTM 438

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QS+HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QV+PKSW+AILLTFDN GMWN
Sbjct: 439 QSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKSWSAILLTFDNAGMWN 498

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYT 563
           +RSE  E +YLG+QLYVSVLSP  SLRDEYNIP  T LCG+VKG+PLP  Y+
Sbjct: 499 IRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPAHYS 549

BLAST of CmoCh02G011890 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 644.8 bits (1662), Expect = 6.4e-185
Identity = 303/413 (73.37%), Postives = 349/413 (84.50%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           GLR+NSRLLIPVPYADPEDD T+LI DWY KSHTALK  LDSGRTL  PDG+LINGK+ K
Sbjct: 140 GLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSGRTLGSPDGVLINGKSGK 199

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
             G++ PLFTMKP KTYKYRICNVG +++LNFRIQ H MKLVEMEGSHV+QNDY SLDVH
Sbjct: 200 LGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEMEGSHVLQNDYDSLDVH 259

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQCF+VLVTA+Q  ++YYMVASTRF+K    +  ++ Y      ASSD+P+AP+GWAWS
Sbjct: 260 VGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQASSDIPKAPVGWAWS 319

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
           LNQFR+FRWNLTASAARPNPQGS+ YG INITRTIKL N+   V+GK+R+  NGVSHV+T
Sbjct: 320 LNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVNGKVRFGFNGVSHVDT 379

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           ETPLKLAEYF + EKVFKY+ I DE   +  TT+TV PNVLN T+R FVE++FENHEK +
Sbjct: 380 ETPLKLAEYFGMSEKVFKYNVIKDEPAAK-ITTLTVEPNVLNITFRTFVEVVFENHEKSM 439

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QS+HL+GYSFFAVA EPG+W+PEKR NYNLLDAVSRHT+QV+PKSW+AILLTFDN GMWN
Sbjct: 440 QSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKSWSAILLTFDNAGMWN 499

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCGVVKGMPLPKPYTI 564
           +RSE  E RYLGQQLYVSVLSP  SLRDEYNIP  T LCG+VKG+PLP PYTI
Sbjct: 500 IRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLPLPTPYTI 551

BLAST of CmoCh02G011890 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.5e-125
Identity = 215/400 (53.75%), Postives = 279/400 (69.75%), Query Frame = 0

Query: 151 GLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTALKQLLDSGRTLARPDGILINGKNAK 210
           G+RI+SR LIPVP+  P DDYT+L+GDWY  +H  LK  LD+G  L  PDGILING+   
Sbjct: 142 GIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPLPDGILINGR--- 201

Query: 211 GDGSDEPLFTMKPEKTYKYRICNVGLRTSLNFRIQNHPMKLVEMEGSHVVQNDYQSLDVH 270
              S      ++P KTY+ RI NVGL+ SLNFRIQNH MKLVE+EG + +QN + SLDVH
Sbjct: 202 ---SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNLFSSLDVH 261

Query: 271 VGQCFSVLVTANQEPRDYYMVASTRFMKTIHMSKAIIRYTNGKGPASSDLPEAPMGWAWS 330
           VGQ +SVL+TA+Q  +DYY+V S+RF   I  +  ++ Y+N   P S  +P+ P+  +WS
Sbjct: 262 VGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDGPIKLSWS 321

Query: 331 LNQFRTFRWNLTASAARPNPQGSFRYGSINITRTIKLVNSFGNVDGKLRYAINGVSHVET 390
            NQ R  R NLTAS  RPNPQGS+RYG INITRTI+L N+ G+++GK RYA+N  S    
Sbjct: 322 FNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAVNSASFYPA 381

Query: 391 ETPLKLAEYFKVPEKVFKYDTITDEGLPEGSTTITVAPNVLNATYRNFVEIIFENHEKRL 450
           +TPLKL +YFK+ + V+K  +I+D+     +  I    +V+ A +R FVE+IFEN E  +
Sbjct: 382 DTPLKLVDYFKI-DGVYKPGSISDQ---PTNGAIFPTTSVMQADFRAFVEVIFENSEDIV 441

Query: 451 QSWHLNGYSFFAVAIEPGKWSPEKRTNYNLLDAVSRHTIQVFPKSWAAILLTFDNCGMWN 510
           QSWHL+GYSF+ V +E GKWSP  R  YNL DA+ R TIQV+P+SW AI +  DN GMWN
Sbjct: 442 QSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNVGMWN 501

Query: 511 LRSELTENRYLGQQLYVSVLSPALSLRDEYNIPDRTLLCG 551
           +RSE+ E +YLGQQ Y+ V + + SLRDEY IP   LLCG
Sbjct: 502 MRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q006247.8e-19675.97L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291624.8e-16966.43L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU401.0e-10244.34Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q9FHN61.5e-9842.99Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q8VXX59.2e-9642.72Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1H0Y22.9e-246100.00L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111459149 PE=3 S... [more]
A0A6J1K3U42.1e-24499.03L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490929 PE=3 SV=... [more]
A0A6J1K3U81.1e-24096.69L-ascorbate oxidase homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114909... [more]
A0A6J1K1Q78.0e-23694.92L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111490931 PE=3 SV=... [more]
A0A6J1K1R21.0e-23596.15L-ascorbate oxidase homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114909... [more]
Match NameE-valueIdentityDescription
XP_022957683.16.1e-246100.00L-ascorbate oxidase homolog [Cucurbita moschata] >XP_022958374.1 L-ascorbate oxi... [more]
KAG6605821.13.0e-24599.76L-ascorbate oxidase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023534711.11.1e-24499.03L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo] >XP_023534712.1 L-ascor... [more]
XP_022995355.14.4e-24499.03L-ascorbate oxidase homolog [Cucurbita maxima][more]
XP_023533003.13.1e-24297.82L-ascorbate oxidase homolog, partial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G13390.18.6e-19876.16SKU5 similar 11 [more]
AT1G55570.14.4e-19474.94SKU5 similar 12 [more]
AT1G55560.12.6e-18673.79SKU5 similar 14 [more]
AT3G13400.16.4e-18573.37SKU5 similar 13 [more]
AT1G21860.11.5e-12553.75SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 169..310
e-value: 1.3E-40
score: 139.1
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 393..530
e-value: 1.1E-23
score: 83.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 357..554
e-value: 1.4E-32
score: 115.1
IPR008972CupredoxinGENE3D2.60.40.420coord: 167..347
e-value: 2.6E-36
score: 127.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 359..549
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 166..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 15..126
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 27..75
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..116
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 150..554
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 150..554
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 169..309
e-value: 5.45492E-86
score: 261.183

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G011890.1CmoCh02G011890.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity