CmoCh02G009470 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G009470
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionUPF0160 protein
LocationCmo_Chr02: 5790909 .. 5793208 (-)
RNA-Seq ExpressionCmoCh02G009470
SyntenyCmoCh02G009470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGGGAAGATGAACGATGGAGCACAACAACATTGGGCTCAACCATTCGGATTATCGAAGCCCACGGTAGAATCCAGACCCACTGCCAGAATTAAGCAACAATTTATACAAGGGAGCTGAAAAGACCAGACTTTAATGTTAGGATTCGAAGGCAATGGTCATTCTTGGTAGAGGTTTAGGGTTTAACCACAAGCAATTCTTCTCCTTCCCCAAGTTTTTCTTTCTCCGTCCTTTCATGGCTACTTCTCCTGTCGCTTCCTATTCCACTGGTTCCCCTGGTGGCGCCGTTTCGGTGAAGCGAGTAGGGACTCACCATGGTAGCTTCCATTGCGATGAGGCGCTTGGCTGCTTCATGATTCGCTTGACGCAGAAGTTCTCTAATGCTCAGATTGTTCGAACCCGTGATCCCCAGGTTCGCGTTTTCTATTCCCTGTCTTGTCAAATGAAAATAGAAATTGAGTTGTAGTTCGGTTATAGACTGCTGGGTTTTGCTGAATAGCTGTAGAAATTTCCTAGAATTTGTTTGGGTTTTTTTTTTTCTTATCAGTAATGTAAATCTGGGTTTTTGTTTCTGCCAAGTTGCGAGAGAACTGAGTATTTTCAACAGAGTCTTGTTCGTTTTGTGATGTTTCCTTCTCATTTGTTTGCTTGGTAGTTTATGGACTGGATTTTGGCCTAGGCATGAAACTGTCGTCTCTGATTTGAATTGGTTGCTAGGTATTAGAAGGTCTTGATGCAGTTCTTGATGTTGGGGGCGTGTATGACCCAAGTCATGATCGTTATGACCATCATCAAAAGGGGTTTGAAGAAGTTTTTGGCCATGGTTTCAACACTAAGCTCAGCAGCGCTGGTCTTGTTTATAAGGTAGGTAGTCGCCTTTCCAAGACCATCTCTAAAATTTGACAAGTGATAATATATCCAAGTTCTTACTTGTTCGTCGTTAAACAGTGTTATCGGATTTTCTTTTCATGAATAACCTTTATCATGCTGCTTACAAAAAATTTAGTAGTTATGATTGTTATTGTTGTTGTTGTTCTGCTCCAGCATTTTGGGAAGGAGATTATTGCAAAGGAGCTTCAAGTTGATGAAGGCCACCCAGACGTGCAAAGGCTATTTTTGGCTGTATACAAAAGTTTCATGGAGGTGTGAATTCAATTTTTTGATTAATCCTTTTTATCTCGTTTCCATTGTTAAATATCATGAAAGTTTTAACCTCAAGAATTTTCTGCACGGGGTTGGCTAACGTTTTTTTATATTTATGTCTGTTTATCTTTTGACGTTCGTGCTTATTTTTTCAGGGCATTGATGCTATAGATAACGGTATCAATCAGTATGATACAGACCAGCCACCAAAATATGTGAATAACACACACCTATCTTCAAGGGTGGGGAGATTGAATCTGGACTGGATTGATCCCGATCAATCATCCGAGAATGAGAATAAAGCCTTTGAGAAAGCAATGGCTCTGGCTGGCAGCGAGTTCTTAGATGTGAGGTTTCTTGAGTGTCTATGGATACAAAATAGTCTCTTAAAATCCTGAACCCAACCATGTTTCCTGAGCTTGTTTACTTGGTTGAGTTGCTTCCTGATCCTTGCTTAATTTCAGAGTGTTCGGTTTCATGCCAAATCATGGCTACCAGCAAGGTCGATCGTGATGAGATGTCTTGGAGCAAGACATGATATCGACCCTAGTGGAGAAATAATGGTTTTGACAACATTTTGCCCTGTAAGTACTCACTTGTTTATCGTTTTAACTATGGTTTTCTTCATTCACTTTGTTTTTTTTTCATCTTGTCATTGGAGAGGTTAGGACATACAAGTTACAAGTTGTATATGCTTTATATCAGTAACGATTACGTAATTTTCTTTTAACAATAGTGGAAACTTCATCTATTTGAGCTCGAGACGGAGTTGAAGAACGACAATTTGATCAAATATGTGCTCTATCAGGTATATGTAACTTCCAACAATTTAAGCCGACAGAGCAGAGCTATCAGAAATGTTTTGTTATTTTCCATTTTAATGCGTTGTGATTTTCAGGACGATCGAAGCAAGCAGTGGCGAGTGCAGGCAGTGGCAATAGCTCCTGACAGATTCGAGAGTCGCAAGCCTCTGCCTGCCCAATGGCGAGGTTTAAGGGACGAGGAACTCTCAAAAGAGTCGGGGATCCCCGGTTGCGTGTTTGTTCATATGAGTGGCTTTATAGGTGGAAATCAAACTTACGAAGGGGCTCTTGCCATGGCGAAGCAAGCATTGAAGCTGTAG

mRNA sequence

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAGGGAAGATGAACGATGGAGCACAACAACATTGGGCTCAACCATTCGGATTATCGAAGCCCACGGTAGAATCCAGACCCACTGCCAGAATTAAGCAACAATTTATACAAGGGAGCTGAAAAGACCAGACTTTAATGTTAGGATTCGAAGGCAATGGTCATTCTTGGTAGAGGTTTAGGGTTTAACCACAAGCAATTCTTCTCCTTCCCCAAGTTTTTCTTTCTCCGTCCTTTCATGGCTACTTCTCCTGTCGCTTCCTATTCCACTGGTTCCCCTGGTGGCGCCGTTTCGGTGAAGCGAGTAGGGACTCACCATGGTAGCTTCCATTGCGATGAGGCGCTTGGCTGCTTCATGATTCGCTTGACGCAGAAGTTCTCTAATGCTCAGATTGTTCGAACCCGTGATCCCCAGGTATTAGAAGGTCTTGATGCAGTTCTTGATGTTGGGGGCGTGTATGACCCAAGTCATGATCGTTATGACCATCATCAAAAGGGGTTTGAAGAAGTTTTTGGCCATGGTTTCAACACTAAGCTCAGCAGCGCTGGTCTTGTTTATAAGCATTTTGGGAAGGAGATTATTGCAAAGGAGCTTCAAGTTGATGAAGGCCACCCAGACGTGCAAAGGCTATTTTTGGCTGTATACAAAAGTTTCATGGAGGGCATTGATGCTATAGATAACGGTATCAATCAGTATGATACAGACCAGCCACCAAAATATGTGAATAACACACACCTATCTTCAAGGGTGGGGAGATTGAATCTGGACTGGATTGATCCCGATCAATCATCCGAGAATGAGAATAAAGCCTTTGAGAAAGCAATGGCTCTGGCTGGCAGCGAGTTCTTAGATAGTGTTCGGTTTCATGCCAAATCATGGCTACCAGCAAGGTCGATCGTGATGAGATGTCTTGGAGCAAGACATGATATCGACCCTAGTGGAGAAATAATGGTTTTGACAACATTTTGCCCTTGGAAACTTCATCTATTTGAGCTCGAGACGGAGTTGAAGAACGACAATTTGATCAAATATGTGCTCTATCAGGTATATGTAACTTCCAACAATTTAAGCCGACAGAGCAGAGCTATCAGAAATGTTTTGTTATTTTCCATTTTAATGCGTTGTGATTTTCAGGACGATCGAAGCAAGCAGTGGCGAGTGCAGGCAGTGGCAATAGCTCCTGACAGATTCGAGAGTCGCAAGCCTCTGCCTGCCCAATGGCGAGGTTTAAGGGACGAGGAACTCTCAAAAGAGTCGGGGATCCCCGGTTGCGTGTTTGTTCATATGAGTGGCTTTATAGGTGGAAATCAAACTTACGAAGGGGCTCTTGCCATGGCGAAGCAAGCATTGAAGCTGTAG

Coding sequence (CDS)

ATGGTCATTCTTGGTAGAGGTTTAGGGTTTAACCACAAGCAATTCTTCTCCTTCCCCAAGTTTTTCTTTCTCCGTCCTTTCATGGCTACTTCTCCTGTCGCTTCCTATTCCACTGGTTCCCCTGGTGGCGCCGTTTCGGTGAAGCGAGTAGGGACTCACCATGGTAGCTTCCATTGCGATGAGGCGCTTGGCTGCTTCATGATTCGCTTGACGCAGAAGTTCTCTAATGCTCAGATTGTTCGAACCCGTGATCCCCAGGTATTAGAAGGTCTTGATGCAGTTCTTGATGTTGGGGGCGTGTATGACCCAAGTCATGATCGTTATGACCATCATCAAAAGGGGTTTGAAGAAGTTTTTGGCCATGGTTTCAACACTAAGCTCAGCAGCGCTGGTCTTGTTTATAAGCATTTTGGGAAGGAGATTATTGCAAAGGAGCTTCAAGTTGATGAAGGCCACCCAGACGTGCAAAGGCTATTTTTGGCTGTATACAAAAGTTTCATGGAGGGCATTGATGCTATAGATAACGGTATCAATCAGTATGATACAGACCAGCCACCAAAATATGTGAATAACACACACCTATCTTCAAGGGTGGGGAGATTGAATCTGGACTGGATTGATCCCGATCAATCATCCGAGAATGAGAATAAAGCCTTTGAGAAAGCAATGGCTCTGGCTGGCAGCGAGTTCTTAGATAGTGTTCGGTTTCATGCCAAATCATGGCTACCAGCAAGGTCGATCGTGATGAGATGTCTTGGAGCAAGACATGATATCGACCCTAGTGGAGAAATAATGGTTTTGACAACATTTTGCCCTTGGAAACTTCATCTATTTGAGCTCGAGACGGAGTTGAAGAACGACAATTTGATCAAATATGTGCTCTATCAGGTATATGTAACTTCCAACAATTTAAGCCGACAGAGCAGAGCTATCAGAAATGTTTTGTTATTTTCCATTTTAATGCGTTGTGATTTTCAGGACGATCGAAGCAAGCAGTGGCGAGTGCAGGCAGTGGCAATAGCTCCTGACAGATTCGAGAGTCGCAAGCCTCTGCCTGCCCAATGGCGAGGTTTAAGGGACGAGGAACTCTCAAAAGAGTCGGGGATCCCCGGTTGCGTGTTTGTTCATATGAGTGGCTTTATAGGTGGAAATCAAACTTACGAAGGGGCTCTTGCCATGGCGAAGCAAGCATTGAAGCTGTAG

Protein sequence

MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL
Homology
BLAST of CmoCh02G009470 vs. ExPASy Swiss-Prot
Match: Q9HB07 (MYG1 exonuclease OS=Homo sapiens OX=9606 GN=MYG1 PE=1 SV=3)

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-84
Identity = 163/356 (45.79%), Postives = 215/356 (60.39%), Query Frame = 0

Query: 49  RVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRY 108
           R+GTH+G+FHCDEAL C ++RL  ++ +A+IVRTRDP+ L   D V+DVGG YDP   RY
Sbjct: 46  RIGTHNGTFHCDEALACALLRLLPEYRDAEIVRTRDPEKLASCDIVVDVGGEYDPRRHRY 105

Query: 109 DHHQKGFEEVF-----GHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVY 168
           DHHQ+ F E       G  + TKLSSAGL+Y HFG +++A+ L   E    V  L+  +Y
Sbjct: 106 DHHQRSFTETMSSLSPGKPWQTKLSSAGLIYLHFGHKLLAQLLGTSEEDSMVGTLYDKMY 165

Query: 169 KSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAM 228
           ++F+E +DA+DNGI+Q+  +  P+Y   T LS+RV RLN  W  PDQ +E     F++AM
Sbjct: 166 ENFVEEVDAVDNGISQW-AEGEPRYALTTTLSARVARLNPTWNHPDQDTE---AGFKRAM 225

Query: 229 ALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTT-FCPWKLHLFELET 288
            L   EFL  + F+  SWLPAR++V   L  R  +DPSGEI+ L    CPWK HL+ LE+
Sbjct: 226 DLVQEEFLQRLDFYQHSWLPARALVEEALAQRFQVDPSGEIVELAKGACPWKEHLYHLES 285

Query: 289 ELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAP 348
            L     I +V+Y                                D++ QWR+Q V   P
Sbjct: 286 GLSPPVAIFFVIY-------------------------------TDQAGQWRIQCVPKEP 345

Query: 349 DRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
             F+SR PLP  WRGLRDE L + SGIPGC+FVH SGF GG+ T EGAL+MA+  L
Sbjct: 346 HSFQSRLPLPEPWRGLRDEALDQVSGIPGCIFVHASGFTGGHHTREGALSMARATL 366

BLAST of CmoCh02G009470 vs. ExPASy Swiss-Prot
Match: Q9JK81 (MYG1 exonuclease OS=Mus musculus OX=10090 GN=Myg1 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-84
Identity = 163/356 (45.79%), Postives = 216/356 (60.67%), Query Frame = 0

Query: 49  RVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRY 108
           R+GTH+G+FHCDEAL C ++RL  +++NA+IVRTRDP+ L   D V+DVGG Y+P   RY
Sbjct: 45  RIGTHNGTFHCDEALACALLRLLPEYANAEIVRTRDPEKLASCDIVVDVGGEYNPQSHRY 104

Query: 109 DHHQKGFEEVF-----GHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVY 168
           DHHQ+ F E       G  + TKLSSAGLVY HFG++++A+ L   E    V  ++  +Y
Sbjct: 105 DHHQRTFTETMSSLCPGKPWQTKLSSAGLVYLHFGRKLLAQLLGTSEEDSVVDTIYDKMY 164

Query: 169 KSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAM 228
           ++F+E +DA+DNGI+Q+  +  P+Y   T LS+RV RLN  W  P+Q +E     F +AM
Sbjct: 165 ENFVEEVDAVDNGISQW-AEGEPRYAMTTTLSARVARLNPTWNQPNQDTE---AGFRRAM 224

Query: 229 ALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTT-FCPWKLHLFELET 288
            L   EFL  + F+  SWLPAR++V   L  R  +D SGEI+ L    CPWK HL+ LE+
Sbjct: 225 DLVQEEFLQRLNFYQHSWLPARALVEEALAQRFKVDSSGEIVELAKGGCPWKEHLYHLES 284

Query: 289 ELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAP 348
           EL     I +V+Y                                D++ QWRVQ V   P
Sbjct: 285 ELSPKVAITFVIY-------------------------------TDQAGQWRVQCVPKEP 344

Query: 349 DRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
             F+SR PLP  WRGLRD+ L + SGIPGC+FVH SGFIGG+ T EGAL MA+  L
Sbjct: 345 HSFQSRLPLPEPWRGLRDKALDQVSGIPGCIFVHASGFIGGHHTREGALNMARATL 365

BLAST of CmoCh02G009470 vs. ExPASy Swiss-Prot
Match: Q641W2 (MYG1 exonuclease OS=Rattus norvegicus OX=10116 GN=Myg1 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.4e-84
Identity = 165/356 (46.35%), Postives = 214/356 (60.11%), Query Frame = 0

Query: 49  RVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRY 108
           R+GTH+G+FHCDEAL C ++RL  ++ NA+IVRTRDP+ L   D V+DVGG Y+P   RY
Sbjct: 46  RIGTHNGTFHCDEALACALLRLLPEYRNAEIVRTRDPEKLALCDIVVDVGGEYNPQRHRY 105

Query: 109 DHHQKGFEEVF-----GHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVY 168
           DHHQ+ F E       G  + TKLSSAGLVY HFG +++A+ L   E    V  ++  +Y
Sbjct: 106 DHHQRTFTETMSSLCPGKPWQTKLSSAGLVYLHFGHKLLAQLLGTSEEDSVVDTIYDKMY 165

Query: 169 KSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAM 228
           ++F+E +DA+DNGI+Q+  +  P+Y   T LS+RV RLN  W  PDQ +E     F +AM
Sbjct: 166 ENFVEEVDAVDNGISQW-AEGEPRYALTTTLSARVSRLNPTWNQPDQDTE---AGFRRAM 225

Query: 229 ALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTT-FCPWKLHLFELET 288
            L   EFL  + F+  SWLPAR++V   L  R  +D SGEI+ L    CPWK HL+ LE+
Sbjct: 226 DLVQEEFLQRLNFYQHSWLPARALVEEALAQRFKVDSSGEIVELAKGGCPWKEHLYHLES 285

Query: 289 ELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAP 348
           EL     I +V+Y                                D++ QWRVQ V   P
Sbjct: 286 ELSPTVAITFVIY-------------------------------TDQAGQWRVQCVPKEP 345

Query: 349 DRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
             F+SR PLP  WRGLRDE L + SGIPGC+FVH SGFIGG+ T EGAL MA+  L
Sbjct: 346 HSFQSRLPLPEPWRGLRDEALDQVSGIPGCIFVHASGFIGGHHTREGALNMARATL 366

BLAST of CmoCh02G009470 vs. ExPASy Swiss-Prot
Match: Q58DG1 (MYG1 exonuclease OS=Bos taurus OX=9913 GN=MYG1 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 7.5e-84
Identity = 163/356 (45.79%), Postives = 217/356 (60.96%), Query Frame = 0

Query: 49  RVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRY 108
           R+GTH+G+FHCDEAL C ++RL  ++  A+IVRTRDP+ L   D V+DVGG YDP   RY
Sbjct: 46  RIGTHNGTFHCDEALACALLRLLPEYREAEIVRTRDPEKLAACDIVVDVGGEYDPQRHRY 105

Query: 109 DHHQKGFEEVF-----GHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVY 168
           DHHQ+ F E       G  + TKLSSAGL+Y HFG +++A+ L   E    V  L+  +Y
Sbjct: 106 DHHQRSFTETMSSLSPGKPWQTKLSSAGLIYLHFGHKLLAQLLGTSEEDGMVGTLYDKMY 165

Query: 169 KSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAM 228
           ++F+E +DA+DNGI+Q++  + P+Y+  T LS+RV RLN  W  P+Q +E     F++AM
Sbjct: 166 ENFVEEVDAVDNGISQWEEGE-PRYLLTTTLSARVARLNPTWNQPNQDTE---AGFKRAM 225

Query: 229 ALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTT-FCPWKLHLFELET 288
            L   EFL  + F+  SWLPAR++V   L  R  +DPSGEI+ L    CPWK HL++LE 
Sbjct: 226 DLVREEFLQRLDFYQNSWLPARTLVEEALAKRFQVDPSGEIIELEKGGCPWKEHLYQLEL 285

Query: 289 ELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAP 348
            L     I +V+Y                                D++ QWRVQ V   P
Sbjct: 286 GLSPAGTIAFVIY-------------------------------TDQAGQWRVQCVPKEP 345

Query: 349 DRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
             F+SR PL   WRGLRDE L + SGIPGC+FVH SGFIGG++T EGAL+MA+  L
Sbjct: 346 HSFQSRLPLLEPWRGLRDEALDQISGIPGCIFVHASGFIGGHRTREGALSMARATL 366

BLAST of CmoCh02G009470 vs. ExPASy Swiss-Prot
Match: Q55G91 (MYG1 protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0267768 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 4.5e-81
Identity = 156/347 (44.96%), Postives = 207/347 (59.65%), Query Frame = 0

Query: 52  THHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHH 111
           TH GSFH DEAL C++++L   + +++I+R+RD  V+E     +DVG VY+    R+DHH
Sbjct: 8   THSGSFHADEALACYLLKLLPTYKDSKIIRSRDKSVIEKSTVAVDVGAVYNFEKLRFDHH 67

Query: 112 QKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGID 171
           Q GF E F    + KLSSAGL+YKH+GK+II + L  ++   ++  L+  +Y S ++ +D
Sbjct: 68  QSGFTETFDDKHDIKLSSAGLIYKHYGKDIIKQRLDTNDSITEL--LYQKLYDSMIQELD 127

Query: 172 AIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFL 231
            +DNG+ +Y +D  P+Y + + +S+RVG LN  W +P Q  E  NK FEKAM L G  FL
Sbjct: 128 GVDNGVERYPSDIKPRYQSGSSISARVGHLNQGWNEP-QDDEIVNKQFEKAMELMGQYFL 187

Query: 232 DSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIK 291
           D + ++ KSWLP RSIV   L  R     SGEI++L  FCPWK HLF LE E      IK
Sbjct: 188 DRLDYYGKSWLPCRSIVENALENRKQTHSSGEILILDMFCPWKDHLFSLEQEKDIKTPIK 247

Query: 292 YVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPL 351
           +VL+                               +D S QWRV AV I    F  R PL
Sbjct: 248 FVLF-------------------------------EDTSGQWRVSAVGINLHSFTLRLPL 307

Query: 352 PAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
           P +WRG RDEELS+ SGI GCVF H +GFIGGN+T EGAL MA + L
Sbjct: 308 PEEWRGKRDEELSQISGIEGCVFAHANGFIGGNKTREGALLMAIKTL 320

BLAST of CmoCh02G009470 vs. ExPASy TrEMBL
Match: A0A6J1H185 (UPF0160 protein OS=Cucurbita moschata OX=3662 GN=LOC111459502 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.0e-213
Identity = 370/400 (92.50%), Postives = 370/400 (92.50%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD
Sbjct: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY
Sbjct: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS
Sbjct: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY     
Sbjct: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 370

BLAST of CmoCh02G009470 vs. ExPASy TrEMBL
Match: A0A6J1K1F2 (UPF0160 protein OS=Cucurbita maxima OX=3661 GN=LOC111491537 PE=3 SV=1)

HSP 1 Score: 748.0 bits (1930), Expect = 2.0e-212
Identity = 367/400 (91.75%), Postives = 368/400 (92.00%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD
Sbjct: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY
Sbjct: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAG EFLDSVRFHAKS
Sbjct: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGGEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY     
Sbjct: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSKQWRVQAVA APDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKQWRVQAVATAPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTYEGA+AMAKQALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGAIAMAKQALKL 370

BLAST of CmoCh02G009470 vs. ExPASy TrEMBL
Match: A0A6J1BNZ6 (UPF0160 protein OS=Momordica charantia OX=3673 GN=LOC111004498 PE=3 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.0e-192
Identity = 332/397 (83.63%), Postives = 344/397 (86.65%), Query Frame = 0

Query: 4   LGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEAL 63
           L RGLGFN KQ F FPKFFFLRPFMA+SPVAS S+ S G  +SVKRVGTHHGSFHCDEAL
Sbjct: 6   LSRGLGFNQKQLFFFPKFFFLRPFMASSPVASVSSPSSGDPISVKRVGTHHGSFHCDEAL 65

Query: 64  GCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGF 123
           GCFMIRLT KFSNAQIVRTRDPQVL+GLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGF
Sbjct: 66  GCFMIRLTDKFSNAQIVRTRDPQVLQGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGF 125

Query: 124 NTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTD 183
           +TKLSSAGLVYKHFGKEIIAKELQVDEGHPDV RLFLAVYKSFME IDA+DNGINQYD D
Sbjct: 126 STKLSSAGLVYKHFGKEIIAKELQVDEGHPDVHRLFLAVYKSFMEAIDAVDNGINQYDID 185

Query: 184 QPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLP 243
           QPPKYVNNTHLSSRVG+LNLDW DPDQS ENENKAFEKAM LAG EFLDSVRFHAKSWLP
Sbjct: 186 QPPKYVNNTHLSSRVGKLNLDWTDPDQSPENENKAFEKAMVLAGGEFLDSVRFHAKSWLP 245

Query: 244 ARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVTSNN 303
           ARSIVM CL AR++IDPSGEIMVLTTFCPWKLHLFELE E+KNDNLIKYVLY        
Sbjct: 246 ARSIVMGCLAARYEIDPSGEIMVLTTFCPWKLHLFELEEEMKNDNLIKYVLY-------- 305

Query: 304 LSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEEL 363
                                 QDDRSK WRVQAVA++PDRFESRKPLPAQWRGLRDEEL
Sbjct: 306 ----------------------QDDRSKHWRVQAVAVSPDRFESRKPLPAQWRGLRDEEL 365

Query: 364 SKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           SKESGIPGCVFVHMSGFIGGNQTYEGAL MAK ALKL
Sbjct: 366 SKESGIPGCVFVHMSGFIGGNQTYEGALTMAKHALKL 372

BLAST of CmoCh02G009470 vs. ExPASy TrEMBL
Match: A0A5A7TL15 (UPF0160 protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001580 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.6e-190
Identity = 330/400 (82.50%), Postives = 344/400 (86.00%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MV L RGLGFN  QF  FPKFFFLR FMA+SP+AS S  SP  ++ VKRVGTHHGSFHCD
Sbjct: 1   MVSLCRGLGFNRSQFLPFPKFFFLRTFMASSPLASLSPASPSDSIPVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLT KFSNAQIVRTRDPQVL+GLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTDKFSNAQIVRTRDPQVLQGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGF+TKLSSAGLVYKHFGKEIIAKELQVDEGHPDV RLFLA+YKSFME IDA+DNGINQY
Sbjct: 121 HGFSTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVHRLFLAIYKSFMEAIDAVDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTD+PPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS
Sbjct: 181 DTDKPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVM  L  RHDIDPSGEIMV+TTFCPWKLHLFELE ELK +N IKYVLY     
Sbjct: 241 WLPARSIVMGSLAGRHDIDPSGEIMVMTTFCPWKLHLFELEAELKIENSIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSK WRVQAVA++PDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKHWRVQAVAVSPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTY+GAL MAK ALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYDGALTMAKNALKL 370

BLAST of CmoCh02G009470 vs. ExPASy TrEMBL
Match: A0A1S3ATP0 (UPF0160 protein-like OS=Cucumis melo OX=3656 GN=LOC103482854 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 6.2e-190
Identity = 329/400 (82.25%), Postives = 343/400 (85.75%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MV L RGLGFN  QF  FPKFFFLR FMA+ P+AS S  SP  ++ VKRVGTHHGSFHCD
Sbjct: 1   MVSLCRGLGFNRSQFLPFPKFFFLRTFMASPPLASLSPASPSDSIPVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLT KFSNAQIVRTRDPQVL+GLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTDKFSNAQIVRTRDPQVLQGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGF+TKLSSAGLVYKHFGKEIIAKELQVDEGHPDV RLFLA+YKSFME IDA+DNGINQY
Sbjct: 121 HGFSTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVHRLFLAIYKSFMEAIDAVDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTD+PPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS
Sbjct: 181 DTDKPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVM  L  RHDIDPSGEIMV+TTFCPWKLHLFELE ELK +N IKYVLY     
Sbjct: 241 WLPARSIVMGSLAGRHDIDPSGEIMVMTTFCPWKLHLFELEAELKIENSIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSK WRVQAVA++PDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKHWRVQAVAVSPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTY+GAL MAK ALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYDGALTMAKNALKL 370

BLAST of CmoCh02G009470 vs. NCBI nr
Match: XP_022958207.1 (UPF0160 protein [Cucurbita moschata])

HSP 1 Score: 752.3 bits (1941), Expect = 2.2e-213
Identity = 370/400 (92.50%), Postives = 370/400 (92.50%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD
Sbjct: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY
Sbjct: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS
Sbjct: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY     
Sbjct: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 370

BLAST of CmoCh02G009470 vs. NCBI nr
Match: XP_023534445.1 (UPF0160 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.6 bits (1934), Expect = 1.4e-212
Identity = 369/400 (92.25%), Postives = 369/400 (92.25%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGA SVKRVGTHHGSFHCD
Sbjct: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGADSVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY
Sbjct: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS
Sbjct: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY     
Sbjct: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 370

BLAST of CmoCh02G009470 vs. NCBI nr
Match: XP_022996257.1 (UPF0160 protein [Cucurbita maxima])

HSP 1 Score: 748.0 bits (1930), Expect = 4.1e-212
Identity = 367/400 (91.75%), Postives = 368/400 (92.00%), Query Frame = 0

Query: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60
           MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD
Sbjct: 1   MVILGRGLGFNHKQFFSFPKFFFLRPFMATSPVASYSTGSPGGAVSVKRVGTHHGSFHCD 60

Query: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120
           EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG
Sbjct: 61  EALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFG 120

Query: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180
           HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY
Sbjct: 121 HGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQY 180

Query: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGSEFLDSVRFHAKS 240
           DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAG EFLDSVRFHAKS
Sbjct: 181 DTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMALAGGEFLDSVRFHAKS 240

Query: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLYQVYVT 300
           WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY     
Sbjct: 241 WLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELKNDNLIKYVLY----- 300

Query: 301 SNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRD 360
                                    QDDRSKQWRVQAVA APDRFESRKPLPAQWRGLRD
Sbjct: 301 -------------------------QDDRSKQWRVQAVATAPDRFESRKPLPAQWRGLRD 360

Query: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQALKL 401
           EELSKESGIPGCVFVHMSGFIGGNQTYEGA+AMAKQALKL
Sbjct: 361 EELSKESGIPGCVFVHMSGFIGGNQTYEGAIAMAKQALKL 370

BLAST of CmoCh02G009470 vs. NCBI nr
Match: KAG6605601.1 (UPF0160 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693.3 bits (1788), Expect = 1.2e-195
Identity = 341/373 (91.42%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 28  MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV 87
           MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV
Sbjct: 1   MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV 60

Query: 88  LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ 147
           LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ
Sbjct: 61  LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ 120

Query: 148 VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWID 207
           VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDW D
Sbjct: 121 VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWTD 180

Query: 208 PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVL 267
           PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLG+RHDIDPSGEIMVL
Sbjct: 181 PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGSRHDIDPSGEIMVL 240

Query: 268 TTFCPWKLHLFELETELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQD 327
           TTFCPWKLHLFELETELKNDNLIKYVLY                              QD
Sbjct: 241 TTFCPWKLHLFELETELKNDNLIKYVLY------------------------------QD 300

Query: 328 DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY 387
           DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY
Sbjct: 301 DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY 343

Query: 388 EGALAMAKQALKL 401
           EGALAMAKQALKL
Sbjct: 361 EGALAMAKQALKL 343

BLAST of CmoCh02G009470 vs. NCBI nr
Match: KAG7035512.1 (UPF0160 protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 693.0 bits (1787), Expect = 1.6e-195
Identity = 340/373 (91.15%), Postives = 342/373 (91.69%), Query Frame = 0

Query: 28  MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV 87
           MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV
Sbjct: 1   MATSPVASYSTGSPGGAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV 60

Query: 88  LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ 147
           LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ
Sbjct: 61  LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ 120

Query: 148 VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWID 207
           VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDW D
Sbjct: 121 VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWTD 180

Query: 208 PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVL 267
           PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLG+RHD+DPSGEIMVL
Sbjct: 181 PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGSRHDVDPSGEIMVL 240

Query: 268 TTFCPWKLHLFELETELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQD 327
           TTFCPWKLHLFELETELKNDNLIKYVLY                              QD
Sbjct: 241 TTFCPWKLHLFELETELKNDNLIKYVLY------------------------------QD 300

Query: 328 DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY 387
           DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY
Sbjct: 301 DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY 343

Query: 388 EGALAMAKQALKL 401
           EGALAMAKQALKL
Sbjct: 361 EGALAMAKQALKL 343

BLAST of CmoCh02G009470 vs. TAIR 10
Match: AT5G41970.1 (Metal-dependent protein hydrolase )

HSP 1 Score: 556.2 bits (1432), Expect = 2.1e-158
Identity = 271/373 (72.65%), Postives = 304/373 (81.50%), Query Frame = 0

Query: 30  TSPVASYSTG-SPG-GAVSVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQV 89
           +S V  YST  SP    +SVK+VGTH+GSFHCDEALGCFMIRL  KFS A IVR+RDP++
Sbjct: 31  SSVVRVYSTATSPSPSEISVKKVGTHNGSFHCDEALGCFMIRLVDKFSGADIVRSRDPKI 90

Query: 90  LEGLDAVLDVGGVYDPSHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQ 149
           L  LDAVLDVGGVYDP HDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKEL 
Sbjct: 91  LAELDAVLDVGGVYDPEHDRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELN 150

Query: 150 VDEGHPDVQRLFLAVYKSFMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWID 209
           V++ HPDV RLFLAVYKSFME IDA+DNGIN+YDTDQPP+YVNNTHLS RVGRLNLDWID
Sbjct: 151 VEQDHPDVLRLFLAVYKSFMEAIDAVDNGINRYDTDQPPRYVNNTHLSPRVGRLNLDWID 210

Query: 210 PDQSSENENKAFEKAMALAGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVL 269
           PDQS E EN+AF++AMALAG EFL+SV+FHA+SWLPARSIVM+CL  R   DPSGEIM+L
Sbjct: 211 PDQSQEKENEAFQRAMALAGKEFLESVQFHARSWLPARSIVMQCLEERFKTDPSGEIMIL 270

Query: 270 TTFCPWKLHLFELETELKNDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQD 329
             FCPWKLHLFELE E+K + LIKYV+Y                              QD
Sbjct: 271 DRFCPWKLHLFELEQEMKIEPLIKYVIY------------------------------QD 330

Query: 330 DRSKQWRVQAVAIAPDRFESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTY 389
           +R+KQWRVQAVA+APDRFE+RKPLP +WRGLRDEELSK + IPGCVFVHMSGFIGGNQ+Y
Sbjct: 331 ERAKQWRVQAVAVAPDRFENRKPLPEKWRGLRDEELSKAAEIPGCVFVHMSGFIGGNQSY 373

Query: 390 EGALAMAKQALKL 401
           +GAL+MA+ AL L
Sbjct: 391 DGALSMAQTALTL 373

BLAST of CmoCh02G009470 vs. TAIR 10
Match: AT3G49320.1 (Metal-dependent protein hydrolase )

HSP 1 Score: 497.7 bits (1280), Expect = 8.9e-141
Identity = 239/353 (67.71%), Postives = 278/353 (78.75%), Query Frame = 0

Query: 46  SVKRVGTHHGSFHCDEALGCFMIRLTQKFSNAQIVRTRDPQVLEGLDAVLDVGGVYDPSH 105
           S KRVGTH+G+FHCDEAL CF++R + +FS+AQIVRTRD QVLE LDA LDVGGVYDP  
Sbjct: 30  STKRVGTHNGTFHCDEALACFILRRSNRFSDAQIVRTRDHQVLEKLDAALDVGGVYDPQS 89

Query: 106 DRYDHHQKGFEEVFGHGFNTKLSSAGLVYKHFGKEIIAKELQVDEGHPDVQRLFLAVYKS 165
           +RYDHHQKGF EVFG GFNTKLSSAGLVYKH+G EII+KELQ+++ HPDV RLFLAVYK+
Sbjct: 90  ERYDHHQKGFSEVFGLGFNTKLSSAGLVYKHYGLEIISKELQLEQRHPDVFRLFLAVYKN 149

Query: 166 FMEGIDAIDNGINQYDTDQPPKYVNNTHLSSRVGRLNLDWIDPDQSSENENKAFEKAMAL 225
           F+E +DA+DNGI+QYDTDQPP+YVNNT L  R+GRLNLDWI+PDQSS  E++AF +AM L
Sbjct: 150 FIEAVDALDNGIHQYDTDQPPRYVNNTSLGHRIGRLNLDWIEPDQSSAKEDEAFHRAMEL 209

Query: 226 AGSEFLDSVRFHAKSWLPARSIVMRCLGARHDIDPSGEIMVLTTFCPWKLHLFELETELK 285
           AGSEFL+ V FHAKSWLPARSIVM CL  R+DID SGEIM L+  CPWKLH+FELE E+K
Sbjct: 210 AGSEFLECVHFHAKSWLPARSIVMECLAKRYDIDSSGEIMKLSKQCPWKLHIFELEEEMK 269

Query: 286 NDNLIKYVLYQVYVTSNNLSRQSRAIRNVLLFSILMRCDFQDDRSKQWRVQAVAIAPDRF 345
            D  IKYVLY                              QDDRS+ WR+QAV+++P+RF
Sbjct: 270 IDPPIKYVLY------------------------------QDDRSENWRIQAVSVSPERF 329

Query: 346 ESRKPLPAQWRGLRDEELSKESGIPGCVFVHMSGFIGGNQTYEGALAMAKQAL 399
           ESRK LP  WRGL  E+LS+ES IP CVFVHMSGFIG NQTYEGALAMA+ +L
Sbjct: 330 ESRKALPLAWRGLEKEKLSEESSIPRCVFVHMSGFIGANQTYEGALAMARASL 352

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9HB073.4e-8445.79MYG1 exonuclease OS=Homo sapiens OX=9606 GN=MYG1 PE=1 SV=3[more]
Q9JK813.4e-8445.79MYG1 exonuclease OS=Mus musculus OX=10090 GN=Myg1 PE=1 SV=1[more]
Q641W23.4e-8446.35MYG1 exonuclease OS=Rattus norvegicus OX=10116 GN=Myg1 PE=1 SV=1[more]
Q58DG17.5e-8445.79MYG1 exonuclease OS=Bos taurus OX=9913 GN=MYG1 PE=2 SV=1[more]
Q55G914.5e-8144.96MYG1 protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0267768 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H1851.0e-21392.50UPF0160 protein OS=Cucurbita moschata OX=3662 GN=LOC111459502 PE=3 SV=1[more]
A0A6J1K1F22.0e-21291.75UPF0160 protein OS=Cucurbita maxima OX=3661 GN=LOC111491537 PE=3 SV=1[more]
A0A6J1BNZ63.0e-19283.63UPF0160 protein OS=Momordica charantia OX=3673 GN=LOC111004498 PE=3 SV=1[more]
A0A5A7TL151.6e-19082.50UPF0160 protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376... [more]
A0A1S3ATP06.2e-19082.25UPF0160 protein-like OS=Cucumis melo OX=3656 GN=LOC103482854 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022958207.12.2e-21392.50UPF0160 protein [Cucurbita moschata][more]
XP_023534445.11.4e-21292.25UPF0160 protein [Cucurbita pepo subsp. pepo][more]
XP_022996257.14.1e-21291.75UPF0160 protein [Cucurbita maxima][more]
KAG6605601.11.2e-19591.42UPF0160 protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7035512.11.6e-19591.15UPF0160 protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G41970.12.1e-15872.65Metal-dependent protein hydrolase [more]
AT3G49320.18.9e-14167.71Metal-dependent protein hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003226Metal-dependent protein hydrolasePFAMPF03690UPF0160coord: 50..296
e-value: 9.4E-97
score: 324.4
coord: 327..398
e-value: 7.6E-31
score: 107.9
IPR003226Metal-dependent protein hydrolasePANTHERPTHR11215METAL DEPENDENT HYDROLASE - RELATEDcoord: 29..297
IPR003226Metal-dependent protein hydrolasePANTHERPTHR11215METAL DEPENDENT HYDROLASE - RELATEDcoord: 325..399
NoneNo IPR availablePANTHERPTHR11215:SF3METAL-DEPENDENT PROTEIN HYDROLASEcoord: 325..399
coord: 29..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G009470.1CmoCh02G009470.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0016787 hydrolase activity