CmoCh02G009220 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G009220
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGlutamine-dependent NAD(+) synthetase
LocationCmo_Chr02: 5668834 .. 5674101 (+)
RNA-Seq ExpressionCmoCh02G009220
SyntenyCmoCh02G009220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAACCCAAACTCCAAACCTGGCTCAGGCTCGAGGACCATGTGACCCGTCGGGCAACCGGCTACCGAATGGAACTTCCGCTTCCAATTTAAAGGTAACCAAAAAGCCAGCCCCGCGGAATACGGCCCAGGAGAATTTCTGGAGGCATAAAAAGGGACCAAACATAAGAACAAGAAAAAGAACAACCCCTCCACTCATCTGCTCCCTTCGTCAAGGTCGGCTCTGCAAACCTCTCTGAATTTTCGCTGGAGTAGCGTAGGTCAGTCCACACAAGCCCTCAATTTCTTGTTCTTCCTTTTTCCCTTTATTTATTTTCTGGTATTGATTCGAATGGGTTCATTGATACTTGATTTTGGTTTGAACAATTGTAAGGTGGGAAGGCTGAGGAATCGTTAATTATGAGGCTGTTGAAAGTAGCAACGTGTAATTTGAATCAATGGGCCATGGATTTTGATTGTAATGTGAAGAACATCAAAGAGTCTATTGACGAGGCTAAACGTGCTGGCGCTGTCATCCGACTTGGCCCTGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCATGCATGCGTAAGTTTTCTGCTTGTTATATCCCAATTTGATTGTTCTATGCGAAAATAGCGACAATTGGTTGTGGATTACTGTTTCAATCGACGTATTCACTGTTAGGAATTACGAACCTCCACAATGGTATGATATCGTCTACTTTGAGCATAAGCTCTGATGATTTTGGATATCGATGGAGATGTATTCCTTAGCTTATAAACTCATGATCAACCCCTTAATTAGTGGACGTGGACTCCTATCCCAACAATCCTCAACACTTACCAGCTTCAAGTTTCTGGGGTGCTCAAATTTCTTGTTTTTGGATACTTTTGCAGATGGGAATGCTTGAAGGATATACTTTCGGGTCCTTTGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAGGACTCTGAGCGTTACAATTGTCAAGTGCTCTGTTTCAATAGGAAAATTATTATGATACGTCCAAAAATGTGGCTTGCAAATGATGGGAATTACAGGGAGCTACGGTGGTTCACAGCATGGAAGCTAAAGGACAAGCTTGTGGATTTTCAGCTGCCTAGAGATATCTCTGAGGCTCTGTCACAAACATCTGTGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACGTGAGTGCCAAAATTACTGGTTTTATTTGTTCTTGTTCTTTTTTCGATCCGAAATTCATATGCATATGTAACTTTTGATTTATGAGCTGATGCCTAGTGTGTTCCTTCTTGTTAGAACTGTTGCTGCTGAAGTTTGTGAAGAACTTTTTACACCTATTCCACCGCATGCTGAACTTGCTTTGAATGGGGTTGAAGTATTTATGAATGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGATGTTCGTCTTCGTGCTTTTATAGATGCTACTCATACTCGTGGTGGAGTTTACATGTACAGTAATCACCAGGGTTGTGACGGTGGCCGTCTTTATTATGGTATACATCTATTGTGTGTACTGTTTATTATCCATATTGACATTTGTAGTTTGCAGTGAGAGACAAGGGATTTATTGAAGTTATGTTTTGTTGTAGATGGTTGTGCTTGTGTTGTTATGAACGGCGAGCTTGTAGCTCAAGGGTCGCAGTTTTCCCTGAAGGATGTCGAGGTTGTGGTTGCCAGTGTAGATCTTGATGCGGTATGAAAACAGTGTGAAGCATGGGATGATTTACTTGATACCTTTATCTTACATTGATTAATGCAAGTTACTTGCCTGCTTTTCGTTCTACTTACGGGGGAGAGAGAAGGAAGGGAAAACGTGAAGTTGTTCCCGGCACAACTGGGAAGTTCTAACATAACTCATTAGTATCATTTGTTTGGTCATATGTTGAACTTAAATTATTGGTGTTTTCTACTCTTTAGGGGTGCTTTATTGGTGTGGTAGATGTCTGGGATACCCTCCAAAGTTCTCGGTTCAAACCCCAAGTAAAACACTCAATAGATTAGAACTCGTGTTATTTCTCAAAACTGGCCTTGTTTGTCCTGGTGTGGTAGATGTCTGAGATACCCTTCAAAGTCCCAGGTTTGAATCCCTAGTAAAACACTCAATAAGTTAGTGTAACAACTCAAGCCCACCGCTAGTAGATATTGTTCGTTTTGGCTTGTTACGTATTGCCATCCGCCTTATAGTTTTAAAACACTTCTGCTATGGAGAGGTTTCCACACCCTTATAAGAAATACTTCGTTCTCATCTCCAATGGATGTGGGATCTCACAATCCACCCCCCTAGGGGGCCCAGCGTCCTCGTTGGCACACCGATCAATGTCTGGCTCTAATACCATTTGTAACAGCTCAAGCCTACCACTAGTAGATATTGTCCGCTTTGGCCCGTTAACATACCGCCGTCAACCTCATGATTTCAAAATGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATGAGGAATGTTTCGTTCTCCTCTCCAACCGATGTGGGAGTGGGCTATGGACATATTAGTGGTGCTCAATTACCCCTGCTTAAAAACAAATATAGTCTTTTTCTTTCTTTCTTTCTTTCTTTTGTGCTAAACATTAAGTTTGAAGAGGCTGGTTTGCCTGTCAGGTTGCTAGTCTTAGAGGATCTATAAGCAGCTTTCAAGAACAAGCAAGCTATAAGACGACCGTTCCTTCTGTGGCAGCCCCATATAGTCTATGTCAGCCATTCAATCTCAAAATTTCTCTTTCGAGTCCCCTTGAGGTTAGTTACTACTTATTCAGTAGCATTTTAGTTCATTATGTCAAAAACGTATCGTTCCCGAGTGTAATGTAATTGATGATTTATAGATTAACTATCACTGCGCGGAAGAGGAAATAGCCTATGGACCTGGTTGCTGGTTGTGGGACTATTTAAGACGAAGCGGAGCTTCTGGATTTTTGCTTCCTCTTTCTGGTGGAGCTGATAGTTCATCTGTTGCAGCCATAGTGGGCTGCATGTGCCAGCTTGTCGTAAAAGGTTTGTTTTTTTAGCACGGGTATTGGTAGAGTTGCCGTAGATATCTATTTTTCAATTTCAGTTAAGTTAGGATGAGAAAAATGAACGAGTGACTGTCGATAAAGTCATTGAATATCGGTGAATAAACGTTTTGCTAATTCTAACATACCCGTCACACTTGTATTCTCTTGGTTGTTTCCATTCATTGGCTTGCAGATATTGCGAAGGGAGACGAACAAGTTAAAGCTGATGCAATACGGATTGGACGTTATGCTGATGGAGAGTTCCCGACTGATAGTAAAGAATTCGCCAAACGAATTTTTTATACTGTATTTATGGGTTCTGAAAATAGGTGAGTAGAATGGCATGAATCTGTAGGTTGTGCACTTGTTCTTTTTGGTATGGTTTGAAATTGCGTATCGTTCACTGACCATGCTTGCTCCTTGTAGTTCTGAAGCAACACGTACACGAGCAAAGGTTCTAGCAGACGAGATTGGTTCATGGCATCTTAATGTTTCCATAGACGGGATTGTTTCTGCACTTTTGTCTTTGTTCCAAACAGTTACTGGAAAACGTCCGCGGTATAAGGTGAAAATACTTGCTTCTTTTCCTTCAAATTTTATTGTATTGCACACACAAGGAGTGGCTAAGGTCATACTCGTCTTGTTTTGCAGTTAGATGGGGGATCTAACGTCGAAAATTTAGGCCTGCAGAATATTCAAGCTCGAATCAGGATGGTTCTGGCTTTTATGTTTGCATCGCTCTTACCTTGGGTACATGGTAAATCTGGATTCTACCTTGTTCTGGGTAGCTCCAACGTCGACGAAGGATTGCGTGGTTACTTGACGAAGGTTTGATATTATTTGAGCTATTTAAGCTGCATTGTTTTCTTTTTATGAGTATCTCATGTTTAGATTCTCGACTCGACACAGTACGATTGCAGTTCGGCAGATCTAAATCCAATTGGAAGCATCAGTAAGCAGGATCTTCGAGCTTTTCTTAGATGGGCTGCCACGCATCTTGGCTTCTCATCGTTGGCAGATATCGAAGCAGCTCCTCCCACAGCCGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGGTTCTCTCTTTCAGTGTTCCACATCTCACATTTTTCTTCAGATAAATTTTATCCTAGAAATGGATTAGAAATTTTTATAAATTGTTCCTGATGTTAATGCTTTTGGATCTATTCTTGCAACCTGTGGTTCCCCTACGGGTGTAAATCAATCGGGTCGGTTTTGCCACCGATCAAACTGTTGGTGAGGTTGGTTCAAATCACTGTTCGACCAACTATAAATGGTCGGTTTAGTTATGTATTTTAACATCGGGTTTGTTGGTTTTGACCAAACCATGCTTGCTGCACATGCTTTTCTGTTTTTAACAAATAGTTGCAGATAGGTAGATTCAATATGTTGTTGCCAGTACTTGTTGTTAAAGTAGTACTATTTTACTTGTAAACAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTACGGAAGGCTGCGAAAAATTTTCCGTTGCGGTCCCGTGTCGATGTTCAAGGTTTGTCGAACATTTTTAGCTTTTACTCACTTGACGATCTCATAATTTTTGGGAACTCTGTCCTTGTTTCAGAATCTCTGCTACCGTTGGGGTTCTAAGTTAACTCCGTCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTACTACTCATTGAATCGACACAAAATGGTCGTTCTAACGCCATCTTATCACGCTGAGGTAAGTATATTCAAAATTGTATTGATACACAATTCATGTTTCTCGTTCTTTAGAAGCTTTTACAAAAATCCCGTTGCAGAGTTATTCCCCGGATGACAATCGGTACGATCTTCGTCAATTTCTGTACAACTCGAGGTGGCCATATCAGTTTCGAAAGATGGATAATCTTGTGAAGGAGTTGAATGGAGACAGTATTGCTATTAAGGAACCAAGTGGGATGGGGGTCGTAGCAGCAGGATCAGGGAACCCCAATGTTGGACTTTAGTTTATAAGCAAGCAAGTTTCTGAGTCTTTTTCTGGTCAAATTGGATGCCACAGGAATTGGACTTTTTTGTCATAATAATGTCTTCTTTATTTGTTCAGAGAATAAGAAAGAGAAAAAAAGTTAATTTTTTCTTTTTTTTTTTTGTTAAGGATTAATAAAATTATAGTAGGAATTATTTGAG

mRNA sequence

ATGTCAAACCCAAACTCCAAACCTGGCTCAGGCTCGAGGACCATGTCGGCTCTGCAAACCTCTCTGAATTTTCGCTGGAGTAGCGTAGGTGGGAAGGCTGAGGAATCGTTAATTATGAGGCTGTTGAAAGTAGCAACGTGTAATTTGAATCAATGGGCCATGGATTTTGATTGTAATGTGAAGAACATCAAAGAGTCTATTGACGAGGCTAAACGTGCTGGCGCTGTCATCCGACTTGGCCCTGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCATGCATGCATGGGAATGCTTGAAGGATATACTTTCGGGTCCTTTGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAGGACTCTGAGCGTTACAATTGTCAAGTGCTCTGTTTCAATAGGAAAATTATTATGATACGTCCAAAAATGTGGCTTGCAAATGATGGGAATTACAGGGAGCTACGGTGGTTCACAGCATGGAAGCTAAAGGACAAGCTTGTGGATTTTCAGCTGCCTAGAGATATCTCTGAGGCTCTGTCACAAACATCTGTGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACAACTGTTGCTGCTGAAGTTTGTGAAGAACTTTTTACACCTATTCCACCGCATGCTGAACTTGCTTTGAATGGGGTTGAAGTATTTATGAATGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGATGTTCGTCTTCGTGCTTTTATAGATGCTACTCATACTCGTGGTGGAGTTTACATGTACAGTAATCACCAGGGTTGTGACGGTGGCCGTCTTTATTATGATGGTTGTGCTTGTGTTGTTATGAACGGCGAGCTTGTAGCTCAAGGGTCGCAGTTTTCCCTGAAGGATGTCGAGGTTGTGGTTGCCAGTGTAGATCTTGATGCGGTTGCTAGTCTTAGAGGATCTATAAGCAGCTTTCAAGAACAAGCAAGCTATAAGACGACCGTTCCTTCTGTGGCAGCCCCATATAGTCTATGTCAGCCATTCAATCTCAAAATTTCTCTTTCGAGTCCCCTTGAGATTAACTATCACTGCGCGGAAGAGGAAATAGCCTATGGACCTGGTTGCTGGTTGTGGGACTATTTAAGACGAAGCGGAGCTTCTGGATTTTTGCTTCCTCTTTCTGGTGGAGCTGATAGTTCATCTGTTGCAGCCATAGTGGGCTGCATGTGCCAGCTTGTCGTAAAAGATATTGCGAAGGGAGACGAACAAGTTAAAGCTGATGCAATACGGATTGGACGTTATGCTGATGGAGAGTTCCCGACTGATAGTAAAGAATTCGCCAAACGAATTTTTTATACTGTATTTATGGGTTCTGAAAATAGTTCTGAAGCAACACGTACACGAGCAAAGGTTCTAGCAGACGAGATTGGTTCATGGCATCTTAATGTTTCCATAGACGGGATTGTTTCTGCACTTTTGTCTTTGTTCCAAACAGTTACTGGAAAACGTCCGCGGTATAAGTTAGATGGGGGATCTAACGTCGAAAATTTAGGCCTGCAGAATATTCAAGCTCGAATCAGGATGGTTCTGGCTTTTATGTTTGCATCGCTCTTACCTTGGGTACATGGTAAATCTGGATTCTACCTTGTTCTGGGTAGCTCCAACGTCGACGAAGGATTGCGTGGTTACTTGACGAAGTACGATTGCAGTTCGGCAGATCTAAATCCAATTGGAAGCATCAGTAAGCAGGATCTTCGAGCTTTTCTTAGATGGGCTGCCACGCATCTTGGCTTCTCATCGTTGGCAGATATCGAAGCAGCTCCTCCCACAGCCGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTACGGAAGGCTGCGAAAAATTTTCCGTTGCGGTCCCGTGTCGATGTTCAAGAATCTCTGCTACCGTTGGGGTTCTAAGTTAACTCCGTCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTACTACTCATTGAATCGACACAAAATGGTCGTTCTAACGCCATCTTATCACGCTGAGAGTTATTCCCCGGATGACAATCGGTACGATCTTCGTCAATTTCTGTACAACTCGAGGTGGCCATATCAGTTTCGAAAGATGGATAATCTTGTGAAGGAGTTGAATGGAGACAGTATTGCTATTAAGGAACCAAGTGGGATGGGGGTCGTAGCAGCAGGATCAGGGAACCCCAATGTTGGACTTTAGTTTATAAGCAAGCAAGTTTCTGAGTCTTTTTCTGGTCAAATTGGATGCCACAGGAATTGGACTTTTTTGTCATAATAATGTCTTCTTTATTTGTTCAGAGAATAAGAAAGAGAAAAAAAGTTAATTTTTTCTTTTTTTTTTTTGTTAAGGATTAATAAAATTATAGTAGGAATTATTTGAG

Coding sequence (CDS)

ATGTCAAACCCAAACTCCAAACCTGGCTCAGGCTCGAGGACCATGTCGGCTCTGCAAACCTCTCTGAATTTTCGCTGGAGTAGCGTAGGTGGGAAGGCTGAGGAATCGTTAATTATGAGGCTGTTGAAAGTAGCAACGTGTAATTTGAATCAATGGGCCATGGATTTTGATTGTAATGTGAAGAACATCAAAGAGTCTATTGACGAGGCTAAACGTGCTGGCGCTGTCATCCGACTTGGCCCTGAACTCGAGATTACTGGATATGGCTGTGAAGACCATTTCTTGGAGCTCGACACTGTCATGCATGCATGGGAATGCTTGAAGGATATACTTTCGGGTCCTTTGACTGATGGGATATTGTGCAGCATTGGCATGCCTGTTATTAAGGACTCTGAGCGTTACAATTGTCAAGTGCTCTGTTTCAATAGGAAAATTATTATGATACGTCCAAAAATGTGGCTTGCAAATGATGGGAATTACAGGGAGCTACGGTGGTTCACAGCATGGAAGCTAAAGGACAAGCTTGTGGATTTTCAGCTGCCTAGAGATATCTCTGAGGCTCTGTCACAAACATCTGTGCCTTTTGGCTATGGATATATTCAGTTTCTAGACACAACTGTTGCTGCTGAAGTTTGTGAAGAACTTTTTACACCTATTCCACCGCATGCTGAACTTGCTTTGAATGGGGTTGAAGTATTTATGAATGCTAGTGGAAGTCATCACCAACTAAGAAAACTTGATGTTCGTCTTCGTGCTTTTATAGATGCTACTCATACTCGTGGTGGAGTTTACATGTACAGTAATCACCAGGGTTGTGACGGTGGCCGTCTTTATTATGATGGTTGTGCTTGTGTTGTTATGAACGGCGAGCTTGTAGCTCAAGGGTCGCAGTTTTCCCTGAAGGATGTCGAGGTTGTGGTTGCCAGTGTAGATCTTGATGCGGTTGCTAGTCTTAGAGGATCTATAAGCAGCTTTCAAGAACAAGCAAGCTATAAGACGACCGTTCCTTCTGTGGCAGCCCCATATAGTCTATGTCAGCCATTCAATCTCAAAATTTCTCTTTCGAGTCCCCTTGAGATTAACTATCACTGCGCGGAAGAGGAAATAGCCTATGGACCTGGTTGCTGGTTGTGGGACTATTTAAGACGAAGCGGAGCTTCTGGATTTTTGCTTCCTCTTTCTGGTGGAGCTGATAGTTCATCTGTTGCAGCCATAGTGGGCTGCATGTGCCAGCTTGTCGTAAAAGATATTGCGAAGGGAGACGAACAAGTTAAAGCTGATGCAATACGGATTGGACGTTATGCTGATGGAGAGTTCCCGACTGATAGTAAAGAATTCGCCAAACGAATTTTTTATACTGTATTTATGGGTTCTGAAAATAGTTCTGAAGCAACACGTACACGAGCAAAGGTTCTAGCAGACGAGATTGGTTCATGGCATCTTAATGTTTCCATAGACGGGATTGTTTCTGCACTTTTGTCTTTGTTCCAAACAGTTACTGGAAAACGTCCGCGGTATAAGTTAGATGGGGGATCTAACGTCGAAAATTTAGGCCTGCAGAATATTCAAGCTCGAATCAGGATGGTTCTGGCTTTTATGTTTGCATCGCTCTTACCTTGGGTACATGGTAAATCTGGATTCTACCTTGTTCTGGGTAGCTCCAACGTCGACGAAGGATTGCGTGGTTACTTGACGAAGTACGATTGCAGTTCGGCAGATCTAAATCCAATTGGAAGCATCAGTAAGCAGGATCTTCGAGCTTTTCTTAGATGGGCTGCCACGCATCTTGGCTTCTCATCGTTGGCAGATATCGAAGCAGCTCCTCCCACAGCCGAGCTGGAGCCAATTCGTTCCAACTACAGCCAGTTGGATGAAGTTGACATGGGAATGACATATGAGGAGCTCTCAGTCTACGGAAGGCTGCGAAAAATTTTCCGTTGCGGTCCCGTGTCGATGTTCAAGAATCTCTGCTACCGTTGGGGTTCTAAGTTAACTCCGTCAGAGGTGGCCGAAAAAGTGAAACATTTCTTCAAGTACTACTCATTGAATCGACACAAAATGGTCGTTCTAACGCCATCTTATCACGCTGAGAGTTATTCCCCGGATGACAATCGGTACGATCTTCGTCAATTTCTGTACAACTCGAGGTGGCCATATCAGTTTCGAAAGATGGATAATCTTGTGAAGGAGTTGAATGGAGACAGTATTGCTATTAAGGAACCAAGTGGGATGGGGGTCGTAGCAGCAGGATCAGGGAACCCCAATGTTGGACTTTAG

Protein sequence

MSNPNSKPGSGSRTMSALQTSLNFRWSSVGGKAEESLIMRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDGNYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTPIPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLYYDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSVAAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL
Homology
BLAST of CmoCh02G009220 vs. ExPASy Swiss-Prot
Match: Q9C723 (Glutamine-dependent NAD(+) synthetase OS=Arabidopsis thaliana OX=3702 GN=At1g55090 PE=2 SV=1)

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 594/725 (81.93%), Postives = 658/725 (90.76%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDF+ N+KNIK SI EAK AGAVIRLGPELE+TGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFESNMKNIKASIAEAKAAGAVIRLGPELEVTGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TV HAWECLK++L G  TD ILCSIGMPVIK +ERYNCQVLC NR+IIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKELLLGDWTDDILCSIGMPVIKGAERYNCQVLCMNRRIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWK +++L +FQLP +ISEAL Q SVPFGYGYIQF+DT VAAEVCEELF+P
Sbjct: 121 NYRELRWFTAWKQREELEEFQLPIEISEALEQKSVPFGYGYIQFIDTAVAAEVCEELFSP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           +PPHAELALNGVEVFMNASGSHHQLRKLD+RL AF+ ATH RGGVYMYSN QGCDG RLY
Sbjct: 181 LPPHAELALNGVEVFMNASGSHHQLRKLDIRLNAFMGATHARGGVYMYSNQQGCDGSRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCAC+V+NG +VAQGSQFSL+DVEV+++ VDLDAVASLRGSISSFQEQAS K  V SV
Sbjct: 241 YDGCACIVVNGNVVAQGSQFSLRDVEVIISQVDLDAVASLRGSISSFQEQASCKVKVSSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           A P  L Q FNLK++LSSP +I YH  +EEIA+GP CW+WDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPCRLTQSFNLKMTLSSPKKIIYHSPQEEIAFGPACWMWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVK+IAKGDEQVKADA RIG YA+G+FPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIAKGDEQVKADANRIGNYANGQFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE T+ R+K LADEIG+WHL+V IDG+VSA+LSLFQTVTGKRPRYK+DGGSN ENLG
Sbjct: 421 ENSSEETKRRSKQLADEIGAWHLDVCIDGVVSAVLSLFQTVTGKRPRYKVDGGSNAENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQAR+RMVLAFM ASLLPWVH K GFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARMRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISK DLR FL+WAAT+LG+ SLA+IEAAPPTAELEPIRS+YSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRLFLKWAATNLGYPSLAEIEAAPPTAELEPIRSDYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GR+RKIFRCGPVSMFKNLCY+WG+KL+P+EVAEKVK+FFKYYS+NRHKM VLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYKWGTKLSPAEVAEKVKYFFKYYSINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSG-----MGVVAAGSGN 758
           YSP+DNR+DLRQFLYNS+WPYQF+K+D +V  LNGDS+A  E        +GVVAA SG+
Sbjct: 661 YSPEDNRFDLRQFLYNSKWPYQFKKIDEIVDSLNGDSVAFPEEEANSNKEIGVVAANSGD 720

BLAST of CmoCh02G009220 vs. ExPASy Swiss-Prot
Match: A2YII8 (Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. indica OX=39946 GN=OsI_25032 PE=3 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 550/734 (74.93%), Postives = 628/734 (85.56%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLL+VATCNLNQWAMDFD N++N+KESI  AK AGA +R+GPELE+TGYGCEDHFLE D
Sbjct: 1   MRLLRVATCNLNQWAMDFDTNLRNVKESIARAKAAGAAVRVGPELELTGYGCEDHFLEQD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           T  HAWECLKDILSG  TDGILCSIGMPVI  S RYNCQV C N KI+MIRPK+ LANDG
Sbjct: 61  TAAHAWECLKDILSGGYTDGILCSIGMPVIFKSVRYNCQVFCLNSKIVMIRPKISLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRE RWF+AW  KD LVDFQLP DISE  SQ +VPFGYG+IQFLD ++A+E CEELFT 
Sbjct: 121 NYREFRWFSAWTFKDALVDFQLPLDISEVTSQDTVPFGYGFIQFLDVSLASETCEELFTA 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
             P  +LALNGVEVF+NASGSHHQLRKL +R+ +  +AT   GGVYMY+N QGCDGGRLY
Sbjct: 181 NAPRIDLALNGVEVFVNASGSHHQLRKLSLRIDSMRNATLACGGVYMYANQQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGC C+ +NG++VAQGSQFSLKDVEV+ A VDLDAV+S R S+SSF+EQAS++T VP V
Sbjct: 241 YDGCCCIAVNGDVVAQGSQFSLKDVEVLDALVDLDAVSSYRASVSSFREQASHRTKVPFV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
             PY LC+PF   +  + P+E+ YH  EEEIA+GP CWLWDYLRRS ASGFLLPLSGGAD
Sbjct: 301 KVPYKLCKPFQSGMVPTGPVEVMYHRPEEEIAFGPSCWLWDYLRRSRASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDI  GDEQVKADA+RIG+Y DGEFP DS+E AKR+FYTV+MG+
Sbjct: 361 SSSVAAIVGCMCQLVVKDIENGDEQVKADAMRIGQYKDGEFPKDSRELAKRLFYTVYMGT 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE TR+RAK+LA+EIGS+HL+V ID IVSALLSLF+ +TGKRPRYK+DGGSN ENLG
Sbjct: 421 ENSSEGTRSRAKMLAEEIGSFHLDVPIDSIVSALLSLFERLTGKRPRYKVDGGSNTENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFM ASL+PWVH KSGFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARIRMVLAFMMASLMPWVHNKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           S+SKQDLRAFLRWAA HL +SSLA++EAAPPTAELEPIR++Y+QLDEVDMGMTYEELS+Y
Sbjct: 541 SVSKQDLRAFLRWAAVHLHYSSLAEVEAAPPTAELEPIRADYNQLDEVDMGMTYEELSIY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMF+NLC+RW   L+PSEVA+KVKHFFKYY++NRHKM VLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFQNLCHRWCGTLSPSEVADKVKHFFKYYAINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGD---------------SIAIKEPSG 758
           YSP+DNR+DLRQFLYN+RWPYQFRK+D LV++++ D                +   E  G
Sbjct: 661 YSPEDNRFDLRQFLYNARWPYQFRKIDELVQDMDKDGKWVNSTEGELRRRKGVRSAEGGG 720

BLAST of CmoCh02G009220 vs. ExPASy Swiss-Prot
Match: Q0D8D4 (Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0167100 PE=3 SV=2)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 550/734 (74.93%), Postives = 628/734 (85.56%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLL+VATCNLNQWAMDFD N++N+KESI  AK AGA +R+GPELE+TGYGCEDHFLE D
Sbjct: 1   MRLLRVATCNLNQWAMDFDTNLRNVKESIARAKAAGAAVRVGPELELTGYGCEDHFLEQD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           T  HAWECLKDILSG  TDGILCSIGMPVI  S RYNCQV C N KI+MIRPK+ LANDG
Sbjct: 61  TAAHAWECLKDILSGGYTDGILCSIGMPVIFKSVRYNCQVFCLNSKIVMIRPKISLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRE RWF+AW  KD LVDFQLP DISE  SQ +VPFGYG+IQFLD ++A+E CEELFT 
Sbjct: 121 NYREFRWFSAWTFKDALVDFQLPLDISEVTSQDTVPFGYGFIQFLDVSLASETCEELFTA 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
             P  +LALNGVEVF+NASGSHHQLRKL +R+ +  +AT   GGVYMY+N QGCDGGRLY
Sbjct: 181 NAPRIDLALNGVEVFVNASGSHHQLRKLSLRIDSMRNATLACGGVYMYANQQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGC C+ +NG++VAQGSQFSLKDVEV+ A VDLDAV+S R S+SSF+EQAS++T VP V
Sbjct: 241 YDGCCCIAVNGDVVAQGSQFSLKDVEVLDALVDLDAVSSYRASVSSFREQASHRTKVPFV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
             PY LC+PF   +  + P+E+ YH  EEEIA+GP CWLWDYLRRS ASGFLLPLSGGAD
Sbjct: 301 KVPYKLCKPFQSGMVPTGPVEVMYHRPEEEIAFGPSCWLWDYLRRSRASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDI  GDEQVKADA+RIG+Y DGEFP DS+E AKR+FYTV+MG+
Sbjct: 361 SSSVAAIVGCMCQLVVKDIENGDEQVKADAMRIGQYKDGEFPKDSRELAKRLFYTVYMGT 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE TR+RAK+LA+EIGS+HL+V ID IVSALLSLF+ +TGKRPRYK+DGGSN ENLG
Sbjct: 421 ENSSEGTRSRAKMLAEEIGSFHLDVPIDSIVSALLSLFERLTGKRPRYKVDGGSNTENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFM ASL+PWVH KSGFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARIRMVLAFMMASLMPWVHNKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           S+SKQDLRAFLRWAA HL +SSLA++EAAPPTAELEPIR++Y+QLDEVDMGMTYEELS+Y
Sbjct: 541 SVSKQDLRAFLRWAAVHLHYSSLAEVEAAPPTAELEPIRADYNQLDEVDMGMTYEELSIY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMF+NLC+RW   L+PSEVA+KVKHFFKYY++NRHKM VLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFQNLCHRWCGTLSPSEVADKVKHFFKYYAINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGD---------------SIAIKEPSG 758
           YSP+DNR+DLRQFLYN+RWPYQFRK+D LV++++ D                +   E  G
Sbjct: 661 YSPEDNRFDLRQFLYNARWPYQFRKIDELVQDMDKDGKWVNSTEGELRRRKGVRSAEGGG 720

BLAST of CmoCh02G009220 vs. ExPASy Swiss-Prot
Match: Q54ML1 (Glutamine-dependent NAD(+) synthetase OS=Dictyostelium discoideum OX=44689 GN=nadsyn1 PE=3 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 8.0e-228
Identity = 391/701 (55.78%), Postives = 491/701 (70.04%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           M+ + +ATCNLNQWAMDF  N++ I ESI+ AK  GA  RLGPELEI GYGCEDHFLE D
Sbjct: 1   MKTVTLATCNLNQWAMDFKGNLERIIESINIAKSKGAKYRLGPELEICGYGCEDHFLEQD 60

Query: 99  TVMHAWECLKDILSGP-LTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLAND 158
           T++H W+ L  IL  P LT  IL  +GMPV+    RYNC+V+  N+KI +I+PK  +A D
Sbjct: 61  TMLHCWQSLAVILKDPELTKDILVDVGMPVLHKDVRYNCRVILLNQKIYLIQPKKAMAMD 120

Query: 159 GNYRELRWFTAWKLKDKLVD-FQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELF 218
           GNYRE RWFT W +K ++V+ F LPR IS+   Q     G   I  LDT +++E CEELF
Sbjct: 121 GNYREGRWFTPW-IKPRVVETFYLPRIISQITGQDECQIGDAIISTLDTAISSETCEELF 180

Query: 219 TPIPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGR 278
           TP  PH ++ L+GVE+F N SGSHHQLRKLD R+     AT   GG+Y+YSN QGCDG R
Sbjct: 181 TPNSPHIQMGLDGVEIFTNGSGSHHQLRKLDTRVDLIRSATSKSGGIYLYSNQQGCDGSR 240

Query: 279 LYYDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVP 338
           LYYDG   +++NG+ V+QGSQFSL D+EV+ A+VDL+ V S+R S  +   QA+     P
Sbjct: 241 LYYDGSCMIMINGDCVSQGSQFSLVDIEVITATVDLEDVRSVRASFMARCAQANLTKEFP 300

Query: 339 SVAAPYSL-----CQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLL 398
            V  P  L     C P +  I       INY+   EEI +GP CWLWDYLRRSG SG+ L
Sbjct: 301 RVRCPIQLTHIDYCHPPDRVI------HINYNTPAEEIGFGPACWLWDYLRRSGLSGYFL 360

Query: 399 PLSGGADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIF 458
           PLSGGADS++ AAI+G MCQLV+ D++KG++QV  DA RI    +   PTDS+EFA R+F
Sbjct: 361 PLSGGADSAATAAIIGIMCQLVILDVSKGNKQVLKDAQRITNSPEDYIPTDSREFASRLF 420

Query: 459 YTVFMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGG 518
           +T ++GS+NSS+ TR RA  +A +IGS H  V ID I  +    F  +T K+P+++  GG
Sbjct: 421 FTAYLGSKNSSKETRDRAMEIAKDIGSVHKEVDIDDISQSFNDAFSQITKKQPQFRAHGG 480

Query: 519 SNVENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSS 578
           +  ENL LQN+QAR RMVL++  ASLL W  G+ G  LVLGS+N DE LRGY+TKYDCSS
Sbjct: 481 TPRENLALQNVQARTRMVLSYHLASLLLWEQGRPGSLLVLGSANCDESLRGYMTKYDCSS 540

Query: 579 ADLNPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMT 638
           AD+NPIG +SK DLR+F+ WA       S+  +  A PTAELEPI  NY+Q DE+DMGMT
Sbjct: 541 ADINPIGGMSKIDLRSFIEWAGKFRDMKSILSVLTATPTAELEPITENYTQSDEIDMGMT 600

Query: 639 YEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLT 698
           YEELS++G+LRK+ RCGPVSMF+ L   W + L PS VAEKVK FF YY++NRHK+  LT
Sbjct: 601 YEELSIFGKLRKVNRCGPVSMFERLVADW-AHLEPSVVAEKVKRFFYYYAINRHKLTTLT 660

Query: 699 PSYHAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELN 733
           PSYHAE YSPDDNRYD RQFLYNS+W  QF  +D +V  L+
Sbjct: 661 PSYHAEGYSPDDNRYDHRQFLYNSKWDVQFETIDKIVLRLS 693

BLAST of CmoCh02G009220 vs. ExPASy Swiss-Prot
Match: O74940 (Putative glutamine-dependent NAD(+) synthetase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC553.02 PE=3 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 4.5e-223
Identity = 386/696 (55.46%), Postives = 495/696 (71.12%), Query Frame = 0

Query: 40  RLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDT 99
           R + +A+C LNQWAMDF+ N   I +SI EAKR  A +R+GPELE+TGYGCEDHFLE DT
Sbjct: 3   RYVTIASCQLNQWAMDFEGNRLRIIDSIKEAKRQNASLRVGPELEVTGYGCEDHFLESDT 62

Query: 100 VMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDGN 159
             H+WE L  I+  P    IL  IGMPV+  + R+NC++L  N KI++IRPK+WL +DGN
Sbjct: 63  YYHSWEMLCSIIHDPDCQDILLDIGMPVMHKAMRHNCRILALNGKILLIRPKIWLCDDGN 122

Query: 160 YRELRWFTAWKLKDKLVD-FQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 219
           +RE RWFT W L+ ++V+   LP  ++++L+QT+VP G   +Q  +T V  E CEELFTP
Sbjct: 123 FRESRWFTPW-LRPRVVETHYLPTFVAKSLNQTTVPIGDAILQCNETVVGVETCEELFTP 182

Query: 220 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 279
             PH ++AL+GVE+F+NASGSHH+LRKL  R+    +AT   GG+Y+YSN +GCDGGRLY
Sbjct: 183 NSPHIDMALDGVEIFINASGSHHELRKLTTRVNLIQNATEKCGGIYLYSNQRGCDGGRLY 242

Query: 280 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 339
           YDG + +  NG+++AQG QFSLKDVEV+ A+VD+D V S R    S   Q   + +   +
Sbjct: 243 YDGSSMIFANGKMLAQGHQFSLKDVEVISATVDVDTVRSYRFQ-PSHGIQGVTRPSYERI 302

Query: 340 AAPYSLC---QPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSG 399
              +SL    Q +++    + P+E+     EEEI +GP CWLWDYLRRS A+GF LPLSG
Sbjct: 303 HVNFSLSSYQQDYDIYRKPTDPIEVTIPLPEEEITFGPACWLWDYLRRSHAAGFFLPLSG 362

Query: 400 GADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEF-PTDSKEFAKRIFYTV 459
           G DS S A +V  MC++V K + + D QV +D  RI    D  +  TD K+    +FYT 
Sbjct: 363 GLDSCSTAVLVYSMCRIVCKAMEEDDAQVLSDVRRI--VGDPSYSSTDPKKLLNHLFYTA 422

Query: 460 FMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNV 519
           FMGSE+SS+ TR+RAK L+  IGS+H +V+ID + SA++ LF  VTGK P+++ +GG+N 
Sbjct: 423 FMGSEHSSKETRSRAKELSSLIGSYHTDVNIDTMTSAVVKLFALVTGKTPQFRSNGGTNA 482

Query: 520 ENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADL 579
           ENL LQNIQAR RM+L ++FA LLPWV G SG  LVLGSSNVDE LRGYLTKYDCSSAD+
Sbjct: 483 ENLALQNIQARSRMLLGYLFAQLLPWVRGYSGSLLVLGSSNVDECLRGYLTKYDCSSADI 542

Query: 580 NPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEE 639
           NPIG ISK DL++FLR+A   L    L +   A PTAELEP   +Y Q DE DMGMTY E
Sbjct: 543 NPIGGISKTDLKSFLRYAKEALDLPILQEFLDATPTAELEPTTESYVQSDEADMGMTYAE 602

Query: 640 LSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSY 699
           LSV+GRLRKI +CGP SMF  L ++WG +L+PS+VAEKVK FF YY +NRHKM  LTPSY
Sbjct: 603 LSVFGRLRKISKCGPYSMFTQLMHQWGDRLSPSQVAEKVKRFFHYYGINRHKMTTLTPSY 662

Query: 700 HAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKE 731
           HAE+Y  DDNRYDLRQFLY S W +Q +K+D L  +
Sbjct: 663 HAETYGVDDNRYDLRQFLYPS-WTWQNKKIDALASK 693

BLAST of CmoCh02G009220 vs. ExPASy TrEMBL
Match: A0A6J1H351 (Glutamine-dependent NAD(+) synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459256 PE=3 SV=1)

HSP 1 Score: 1474.1 bits (3815), Expect = 0.0e+00
Identity = 720/720 (100.00%), Postives = 720/720 (100.00%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG
Sbjct: 421 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL
Sbjct: 661 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 720

BLAST of CmoCh02G009220 vs. ExPASy TrEMBL
Match: A0A6J1JYB5 (Glutamine-dependent NAD(+) synthetase OS=Cucurbita maxima OX=3661 GN=LOC111490859 PE=3 SV=1)

HSP 1 Score: 1461.0 bits (3781), Expect = 0.0e+00
Identity = 710/720 (98.61%), Postives = 718/720 (99.72%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRL+KVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLMKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKI+MIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIVMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLP DISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPGDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQPFNLKISLSSPL+INYHC+EEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQPFNLKISLSSPLQINYHCSEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIG YADGEFPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGHYADGEFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATRTRAKVLADEIGSWHL+VSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG
Sbjct: 421 ENSSEATRTRAKVLADEIGSWHLDVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYS+NRHKMVVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSMNRHKMVVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSPDDNRYDLRQFLYNSRWPYQFRKMDN+VKELNGDSIAIKEPSGMGVVAAGSGNPNVG+
Sbjct: 661 YSPDDNRYDLRQFLYNSRWPYQFRKMDNVVKELNGDSIAIKEPSGMGVVAAGSGNPNVGV 720

BLAST of CmoCh02G009220 vs. ExPASy TrEMBL
Match: A0A6J1CXE6 (Glutamine-dependent NAD(+) synthetase OS=Momordica charantia OX=3673 GN=LOC111015310 PE=3 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 687/720 (95.42%), Postives = 707/720 (98.19%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVK+IKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TV HAWECLKDIL GP TDGILCSIGMPVIKDSERYNCQVLC+NRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDT VAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTAVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFI ATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVV+NG+LVAQGSQFSLKDVEVVVA VDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVINGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQ FNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVK+IA GDEQVKADAIRIG YADGEFPTDS+EFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATR+RAKVLADEIGSWHL+VSIDGIVSALLSLFQT+TGKRPRYK+DGGSN+ENLG
Sbjct: 421 ENSSEATRSRAKVLADEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVH KSGFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKSGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLG+SSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYS+NRHKM VLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGTKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSP+DNR+DLRQFLYNSRWPYQFRK+DNLVKELNGDSIAIKE  GMGVVAAGSGNP+VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDNLVKELNGDSIAIKESDGMGVVAAGSGNPSVGL 720

BLAST of CmoCh02G009220 vs. ExPASy TrEMBL
Match: A0A0A0KMF0 (Glutamine-dependent NAD(+) synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G149340 PE=3 SV=1)

HSP 1 Score: 1387.5 bits (3590), Expect = 0.0e+00
Identity = 668/720 (92.78%), Postives = 696/720 (96.67%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVK+IKESIDEAKRAGAVIRLGPELEI+GYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEISGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TV HAWECLKDIL GP TDGILCSIGMPVIKDSERYNCQ++C+NRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQIMCYNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLP+D++EALSQTSVPFGYGYIQF DT VAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALSQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFI ATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVV+NG+LVAQGSQFSLKDVEVVVA VDLDAVASLRGSISSFQEQASYKT VPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTKVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQ FNLKISLSSPLEI YHCAEEEIA+GPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVK+IA GDEQVKADAIRIG YADGE PTDS+EFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGELPTDSREFARRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE TRTRAKVLA EIGSWHL+VSIDGIVSALLSLFQT+TGKRPRYK+DGGSN+ENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVH K GFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISK DLRAFLRWA+THL +SSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GR+RKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYS+NRHKM VLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSP+DNR+DLRQFLYNSRWPYQFRK+D LV+ELNGD IAIKE SGMGVVAAGSGNP VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELNGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of CmoCh02G009220 vs. ExPASy TrEMBL
Match: A0A1S3ATK9 (Glutamine-dependent NAD(+) synthetase OS=Cucumis melo OX=3656 GN=LOC103482827 PE=3 SV=1)

HSP 1 Score: 1386.3 bits (3587), Expect = 0.0e+00
Identity = 669/720 (92.92%), Postives = 695/720 (96.53%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVK+IKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKHIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TV HAWECLKDIL GP TDGILCSIGMPVIKDSERYNCQVLC+NRKIIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKDILLGPWTDGILCSIGMPVIKDSERYNCQVLCYNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLP+D++EAL QTSVPFGYGYIQF DT VAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPKDVAEALLQTSVPFGYGYIQFQDTAVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFI ATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIGATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVV+NG+LVAQGSQFSLKDVEVVVA VDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVVNGDLVAQGSQFSLKDVEVVVAHVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           A PYSLC+ FNLKISLSSP EI YHCAEEEIA+GPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPYSLCESFNLKISLSSPREIKYHCAEEEIAFGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVK+IA GDEQVKADAIRIG YADGEFPTDS+EFA+RIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIANGDEQVKADAIRIGHYADGEFPTDSREFARRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE TRTRAKVLA EIGSWHL+VSIDGIVSALLSLFQT+TGKRPRYK+DGGSN+ENLG
Sbjct: 421 ENSSEETRTRAKVLAHEIGSWHLDVSIDGIVSALLSLFQTLTGKRPRYKVDGGSNIENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVH K GFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISK DLRAFLRWA+THL +SSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRAFLRWASTHLSYSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWG+KLTPSEVAEKVKHFFKYYS+NRHKM VLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGAKLTPSEVAEKVKHFFKYYSINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSP+DNR+DLRQFLYNSRWPYQFRK+D LV+EL+GD IAIKE SGMGVVAAGSGNP VGL
Sbjct: 661 YSPEDNRFDLRQFLYNSRWPYQFRKIDKLVEELDGDGIAIKESSGMGVVAAGSGNPKVGL 720

BLAST of CmoCh02G009220 vs. NCBI nr
Match: KAG6605577.1 (Glutamine-dependent NAD(+) synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1536.5 bits (3977), Expect = 0.0e+00
Identity = 751/758 (99.08%), Postives = 757/758 (99.87%), Query Frame = 0

Query: 1   MSNPNSKPGSGSRTMSALQTSLNFRWSSVGGKAEESLIMRLLKVATCNLNQWAMDFDCNV 60
           MSNPNSKPGSGSRT+SAL+TSLNFRWSSVGGKAEESLIMRLLKVATCNLNQWAMDFDCNV
Sbjct: 1   MSNPNSKPGSGSRTVSALRTSLNFRWSSVGGKAEESLIMRLLKVATCNLNQWAMDFDCNV 60

Query: 61  KNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTVMHAWECLKDILSGPLTDGIL 120
           KNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTVMHAWECLKDILSGPLTDGIL
Sbjct: 61  KNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELDTVMHAWECLKDILSGPLTDGIL 120

Query: 121 CSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDGNYRELRWFTAWKLKDKLVDFQL 180
           CSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDGNYRELRWFTAWKLKDKLVDFQL
Sbjct: 121 CSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDGNYRELRWFTAWKLKDKLVDFQL 180

Query: 181 PRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTPIPPHAELALNGVEVFMNASGSH 240
           PRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTPIPPHAELALNGVEVFMNASGSH
Sbjct: 181 PRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTPIPPHAELALNGVEVFMNASGSH 240

Query: 241 HQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLYYDGCACVVMNGELVAQGSQFSL 300
           HQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLYYDGCACVVMNGELVAQGSQFSL
Sbjct: 241 HQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLYYDGCACVVMNGELVAQGSQFSL 300

Query: 301 KDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSVAAPYSLCQPFNLKISLSSPLEI 360
           KDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSVA+PYSLCQPFNLKISLSSPLEI
Sbjct: 301 KDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSVASPYSLCQPFNLKISLSSPLEI 360

Query: 361 NYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKG 420
           NYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKG
Sbjct: 361 NYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKG 420

Query: 421 DEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGSENSSEATRTRAKVLADEIGSWH 480
           DEQVKADAIRIGRYADGEFPTDS+EFAKR+FYTVFMGSENSSEATRTRAKVLADEIGSWH
Sbjct: 421 DEQVKADAIRIGRYADGEFPTDSREFAKRVFYTVFMGSENSSEATRTRAKVLADEIGSWH 480

Query: 481 LNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPW 540
           LNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPW
Sbjct: 481 LNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPW 540

Query: 541 VHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSS 600
           VHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSS
Sbjct: 541 VHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSS 600

Query: 601 LADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRW 660
           LADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRW
Sbjct: 601 LADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRW 660

Query: 661 GSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQ 720
           GSKLTPSEVAEKVKHFFKYYS+NRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQ
Sbjct: 661 GSKLTPSEVAEKVKHFFKYYSMNRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQ 720

Query: 721 FRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 759
           FRKMDNLVKELNGDSIAIKEPSGMGVVAAGSG PNVGL
Sbjct: 721 FRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGKPNVGL 758

BLAST of CmoCh02G009220 vs. NCBI nr
Match: XP_022957834.1 (glutamine-dependent NAD(+) synthetase-like [Cucurbita moschata])

HSP 1 Score: 1474.1 bits (3815), Expect = 0.0e+00
Identity = 720/720 (100.00%), Postives = 720/720 (100.00%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG
Sbjct: 421 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL
Sbjct: 661 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 720

BLAST of CmoCh02G009220 vs. NCBI nr
Match: XP_023534387.1 (glutamine-dependent NAD(+) synthetase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1468.0 bits (3799), Expect = 0.0e+00
Identity = 717/720 (99.58%), Postives = 718/720 (99.72%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQ FNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQSFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG
Sbjct: 421 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYS+NRHKMVVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSMNRHKMVVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSG PNVGL
Sbjct: 661 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGKPNVGL 720

BLAST of CmoCh02G009220 vs. NCBI nr
Match: XP_022995257.1 (glutamine-dependent NAD(+) synthetase-like [Cucurbita maxima])

HSP 1 Score: 1461.0 bits (3781), Expect = 0.0e+00
Identity = 710/720 (98.61%), Postives = 718/720 (99.72%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRL+KVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLMKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKI+MIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIVMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLP DISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPGDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           AAPYSLCQPFNLKISLSSPL+INYHC+EEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AAPYSLCQPFNLKISLSSPLQINYHCSEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIG YADGEFPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGHYADGEFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSEATRTRAKVLADEIGSWHL+VSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG
Sbjct: 421 ENSSEATRTRAKVLADEIGSWHLDVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG
Sbjct: 481 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYS+NRHKMVVLTPSYHAES
Sbjct: 601 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSMNRHKMVVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSGMGVVAAGSGNPNVGL 758
           YSPDDNRYDLRQFLYNSRWPYQFRKMDN+VKELNGDSIAIKEPSGMGVVAAGSGNPNVG+
Sbjct: 661 YSPDDNRYDLRQFLYNSRWPYQFRKMDNVVKELNGDSIAIKEPSGMGVVAAGSGNPNVGV 720

BLAST of CmoCh02G009220 vs. NCBI nr
Match: KAG7035492.1 (Glutamine-dependent NAD(+) synthetase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 718/741 (96.90%), Postives = 719/741 (97.03%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG
Sbjct: 61  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP
Sbjct: 121 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY
Sbjct: 181 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDA---------------------VAS 338
           YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDA                     VAS
Sbjct: 241 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAGCFIGVVDVWDTLQSSRFKPQVAS 300

Query: 339 LRGSISSFQEQASYKTTVPSVAAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWL 398
           LRGSISSFQEQASYKTTVPSVAAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWL
Sbjct: 301 LRGSISSFQEQASYKTTVPSVAAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWL 360

Query: 399 WDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADG 458
           WDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADG
Sbjct: 361 WDYLRRSGASGFLLPLSGGADSSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADG 420

Query: 459 EFPTDSKEFAKRIFYTVFMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQ 518
           EFPTDSKEFAKRIFYTVFMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQ
Sbjct: 421 EFPTDSKEFAKRIFYTVFMGSENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQ 480

Query: 519 TVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVD 578
           TVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVD
Sbjct: 481 TVTGKRPRYKLDGGSNVENLGLQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVD 540

Query: 579 EGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIR 638
           EGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIR
Sbjct: 541 EGLRGYLTKYDCSSADLNPIGSISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIR 600

Query: 639 SNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFF 698
           SNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFF
Sbjct: 601 SNYSQLDEVDMGMTYEELSVYGRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFF 660

Query: 699 KYYSLNRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIA 758
           KYYS+NRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIA
Sbjct: 661 KYYSMNRHKMVVLTPSYHAESYSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIA 720

BLAST of CmoCh02G009220 vs. TAIR 10
Match: AT1G55090.1 (carbon-nitrogen hydrolase family protein )

HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 594/725 (81.93%), Postives = 658/725 (90.76%), Query Frame = 0

Query: 39  MRLLKVATCNLNQWAMDFDCNVKNIKESIDEAKRAGAVIRLGPELEITGYGCEDHFLELD 98
           MRLLKVATCNLNQWAMDF+ N+KNIK SI EAK AGAVIRLGPELE+TGYGCEDHFLELD
Sbjct: 1   MRLLKVATCNLNQWAMDFESNMKNIKASIAEAKAAGAVIRLGPELEVTGYGCEDHFLELD 60

Query: 99  TVMHAWECLKDILSGPLTDGILCSIGMPVIKDSERYNCQVLCFNRKIIMIRPKMWLANDG 158
           TV HAWECLK++L G  TD ILCSIGMPVIK +ERYNCQVLC NR+IIMIRPKMWLANDG
Sbjct: 61  TVTHAWECLKELLLGDWTDDILCSIGMPVIKGAERYNCQVLCMNRRIIMIRPKMWLANDG 120

Query: 159 NYRELRWFTAWKLKDKLVDFQLPRDISEALSQTSVPFGYGYIQFLDTTVAAEVCEELFTP 218
           NYRELRWFTAWK +++L +FQLP +ISEAL Q SVPFGYGYIQF+DT VAAEVCEELF+P
Sbjct: 121 NYRELRWFTAWKQREELEEFQLPIEISEALEQKSVPFGYGYIQFIDTAVAAEVCEELFSP 180

Query: 219 IPPHAELALNGVEVFMNASGSHHQLRKLDVRLRAFIDATHTRGGVYMYSNHQGCDGGRLY 278
           +PPHAELALNGVEVFMNASGSHHQLRKLD+RL AF+ ATH RGGVYMYSN QGCDG RLY
Sbjct: 181 LPPHAELALNGVEVFMNASGSHHQLRKLDIRLNAFMGATHARGGVYMYSNQQGCDGSRLY 240

Query: 279 YDGCACVVMNGELVAQGSQFSLKDVEVVVASVDLDAVASLRGSISSFQEQASYKTTVPSV 338
           YDGCAC+V+NG +VAQGSQFSL+DVEV+++ VDLDAVASLRGSISSFQEQAS K  V SV
Sbjct: 241 YDGCACIVVNGNVVAQGSQFSLRDVEVIISQVDLDAVASLRGSISSFQEQASCKVKVSSV 300

Query: 339 AAPYSLCQPFNLKISLSSPLEINYHCAEEEIAYGPGCWLWDYLRRSGASGFLLPLSGGAD 398
           A P  L Q FNLK++LSSP +I YH  +EEIA+GP CW+WDYLRRSGASGFLLPLSGGAD
Sbjct: 301 AVPCRLTQSFNLKMTLSSPKKIIYHSPQEEIAFGPACWMWDYLRRSGASGFLLPLSGGAD 360

Query: 399 SSSVAAIVGCMCQLVVKDIAKGDEQVKADAIRIGRYADGEFPTDSKEFAKRIFYTVFMGS 458
           SSSVAAIVGCMCQLVVK+IAKGDEQVKADA RIG YA+G+FPTDSKEFAKRIFYTVFMGS
Sbjct: 361 SSSVAAIVGCMCQLVVKEIAKGDEQVKADANRIGNYANGQFPTDSKEFAKRIFYTVFMGS 420

Query: 459 ENSSEATRTRAKVLADEIGSWHLNVSIDGIVSALLSLFQTVTGKRPRYKLDGGSNVENLG 518
           ENSSE T+ R+K LADEIG+WHL+V IDG+VSA+LSLFQTVTGKRPRYK+DGGSN ENLG
Sbjct: 421 ENSSEETKRRSKQLADEIGAWHLDVCIDGVVSAVLSLFQTVTGKRPRYKVDGGSNAENLG 480

Query: 519 LQNIQARIRMVLAFMFASLLPWVHGKSGFYLVLGSSNVDEGLRGYLTKYDCSSADLNPIG 578
           LQNIQAR+RMVLAFM ASLLPWVH K GFYLVLGSSNVDEGLRGYLTKYDCSSAD+NPIG
Sbjct: 481 LQNIQARMRMVLAFMLASLLPWVHSKPGFYLVLGSSNVDEGLRGYLTKYDCSSADINPIG 540

Query: 579 SISKQDLRAFLRWAATHLGFSSLADIEAAPPTAELEPIRSNYSQLDEVDMGMTYEELSVY 638
           SISK DLR FL+WAAT+LG+ SLA+IEAAPPTAELEPIRS+YSQLDEVDMGMTYEELSVY
Sbjct: 541 SISKMDLRLFLKWAATNLGYPSLAEIEAAPPTAELEPIRSDYSQLDEVDMGMTYEELSVY 600

Query: 639 GRLRKIFRCGPVSMFKNLCYRWGSKLTPSEVAEKVKHFFKYYSLNRHKMVVLTPSYHAES 698
           GR+RKIFRCGPVSMFKNLCY+WG+KL+P+EVAEKVK+FFKYYS+NRHKM VLTPSYHAES
Sbjct: 601 GRMRKIFRCGPVSMFKNLCYKWGTKLSPAEVAEKVKYFFKYYSINRHKMTVLTPSYHAES 660

Query: 699 YSPDDNRYDLRQFLYNSRWPYQFRKMDNLVKELNGDSIAIKEPSG-----MGVVAAGSGN 758
           YSP+DNR+DLRQFLYNS+WPYQF+K+D +V  LNGDS+A  E        +GVVAA SG+
Sbjct: 661 YSPEDNRFDLRQFLYNSKWPYQFKKIDEIVDSLNGDSVAFPEEEANSNKEIGVVAANSGD 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7230.0e+0081.93Glutamine-dependent NAD(+) synthetase OS=Arabidopsis thaliana OX=3702 GN=At1g550... [more]
A2YII80.0e+0074.93Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q0D8D40.0e+0074.93Glutamine-dependent NAD(+) synthetase OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q54ML18.0e-22855.78Glutamine-dependent NAD(+) synthetase OS=Dictyostelium discoideum OX=44689 GN=na... [more]
O749404.5e-22355.46Putative glutamine-dependent NAD(+) synthetase OS=Schizosaccharomyces pombe (str... [more]
Match NameE-valueIdentityDescription
A0A6J1H3510.0e+00100.00Glutamine-dependent NAD(+) synthetase OS=Cucurbita moschata OX=3662 GN=LOC111459... [more]
A0A6J1JYB50.0e+0098.61Glutamine-dependent NAD(+) synthetase OS=Cucurbita maxima OX=3661 GN=LOC11149085... [more]
A0A6J1CXE60.0e+0095.42Glutamine-dependent NAD(+) synthetase OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0KMF00.0e+0092.78Glutamine-dependent NAD(+) synthetase OS=Cucumis sativus OX=3659 GN=Csa_5G149340... [more]
A0A1S3ATK90.0e+0092.92Glutamine-dependent NAD(+) synthetase OS=Cucumis melo OX=3656 GN=LOC103482827 PE... [more]
Match NameE-valueIdentityDescription
KAG6605577.10.0e+0099.08Glutamine-dependent NAD(+) synthetase, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022957834.10.0e+00100.00glutamine-dependent NAD(+) synthetase-like [Cucurbita moschata][more]
XP_023534387.10.0e+0099.58glutamine-dependent NAD(+) synthetase-like [Cucurbita pepo subsp. pepo][more]
XP_022995257.10.0e+0098.61glutamine-dependent NAD(+) synthetase-like [Cucurbita maxima][more]
KAG7035492.10.0e+0096.90Glutamine-dependent NAD(+) synthetase [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
AT1G55090.10.0e+0081.93carbon-nitrogen hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036526Carbon-nitrogen hydrolase superfamilyGENE3D3.60.110.10coord: 37..341
e-value: 2.7E-69
score: 235.5
IPR036526Carbon-nitrogen hydrolase superfamilySUPERFAMILY56317Carbon-nitrogen hydrolasecoord: 39..327
IPR014445Glutamine-dependent NAD(+) synthetasePIRSFPIRSF006630NADS_GATcoord: 39..720
e-value: 3.7E-151
score: 502.3
IPR014445Glutamine-dependent NAD(+) synthetaseHAMAPMF_02090NadE_glutamine_depcoord: 42..718
score: 28.566494
IPR022310NAD/GMP synthasePFAMPF02540NAD_synthasecoord: 376..636
e-value: 1.2E-26
score: 93.3
IPR003694NAD(+) synthetaseTIGRFAMTIGR00552TIGR00552coord: 446..638
e-value: 6.2E-19
score: 66.1
IPR003694NAD(+) synthetasePANTHERPTHR23090NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASEcoord: 39..758
IPR003694NAD(+) synthetaseCDDcd00553NAD_synthasecoord: 366..687
e-value: 7.94931E-89
score: 277.506
IPR003010Carbon-nitrogen hydrolasePFAMPF00795CN_hydrolasecoord: 43..319
e-value: 6.5E-29
score: 101.1
IPR003010Carbon-nitrogen hydrolasePROSITEPS50263CN_HYDROLASEcoord: 42..312
score: 45.197975
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 365..732
e-value: 5.4E-124
score: 415.7
NoneNo IPR availablePANTHERPTHR23090:SF16GLUTAMINE-DEPENDENT NAD(+) SYNTHETASEcoord: 39..758
NoneNo IPR availableCDDcd07570GAT_Gln-NAD-synthcoord: 43..330
e-value: 2.19833E-88
score: 277.043
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 356..699

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G009220.1CmoCh02G009220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009435 NAD biosynthetic process
biological_process GO:0006807 nitrogen compound metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004359 glutaminase activity
molecular_function GO:0003952 NAD+ synthase (glutamine-hydrolyzing) activity