CmoCh02G009180 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G009180
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTrehalase
LocationCmo_Chr02: 5646854 .. 5650606 (+)
RNA-Seq ExpressionCmoCh02G009180
SyntenyCmoCh02G009180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGCCGCGCGCCGCCCGTTGTGTTTGGTACCAAATTTTGATATCAACAGGCGGAGAGAGAGAAAGAAATTGATATCCATTTGTTAATTGGCCTAATCCATTGGACACCATTCATAGCTTCTTTCCAAGATCATAAAAACGAAATTTCCATCTCGATTCTCGATTCATTCTTCTCTCATAATTTTCCCATCAATCCTCGTCATCACCTTCTTCTTCGCCACAAATCCCTCTCTCTCTCTCTCTTCTCTGTACTTTTTTTTCTCTCTCTCCTAGTCCGCACTCTGTCATCACCTCCCCCAGTTTTCTTCCTCCATAAATATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGGTATTGTGATGGCTACAGTTTTGTCTCGATTTCTTTCTGCTTTTTCAATGATTTTATTTTAATCTCCAGCGTTTTAACTTGACTTGTACTTTTCCAAGAAAACTGTTCCTGATTGAATTTGGCCTTTTATAAACTCACTTTTTTCTTCACATTGTTCTTATCTTTGCCGGCCCCTTTTCTTGGATTCTGTGTTTCCCCCTTTTGTCACTTTGTGAAGAAACAATCCTCGGGCTGTTCAACTGGTTTCTGTCAAATATTCCACTGTGAAATTTGGGAAATGCAATTTGAAAATTTCCCATTAAGAAACTGAAAATTTTGTACATTATATTTTCTTGAATGATGACAAAACTGGGTGGGATTGTTCCCTCATTTGTTTTTATTTCATTTTGTGCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGGTAAATTAACTCATCAATCCCTGTGAAGAATGTGTGAAAATTTAGTAGTTTTGGAAACTGATTATCCAACCCTGGACTACATTTTGCAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGTACAACGAGATACCGATGCGTCTTACTCGATCGACCATTATAGCTGTATTGATTTCCTTAGTTTTAGTCAGTTTGTAATTTAGTCTTTCTCTATTGGTTTTAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGTGTGTTTTCCTCCTGGATCTTAGTTACCTACCTGCCATAATATGAACTTACCTTGTGTTTGACTTCATGATTTATTCTTGACAGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGTATAACCATTGTGATTTGGAATATCTGCAGAGCTAAGAGTTTATTAAGTTTTGAGACATTTTTATAGCATTACAGCAATGAAGTTTTTAAACGCTTTTGAGTTTGCTTCTTGAAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGGTATGGCTTGAACAGTTCTTCGAAAACCTCGACGTTCTTCGTATCACCATTAGCACAACTAATGTTTTCTCTGAATTCAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGTATATATATATGCATATAATTTCTGAAAACTTTCTAAGCTTTTGATACTCTTCTTTTAGCAGTTATGTTGATTGAAACTTTTGGAGCTTTCAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGGTTTGTAGCTAATTCCCTCTTTATCATAAGCATTTTTTCAGCTAAGTTTCTCTGAAGGCTAAGGGTAGGAATTGTATGCTTGTTTTTGTATAAACAGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGTTAGTAGTACTGAATCTTTAGGAACTGTTTGGTTTACATGCACAACACTCAAACCTTTCATATCTGTACACAACAAAAGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGGTACATCACTCATCAACTTGCATTGGCATTTCTCTCTTCCACTCATTTGAATCATTAAGAAAAGAAAGTGTTCCTCTGCTGATCTGTTTCCTTTTGGTTCCTATGTTTGAGTGTAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAATTGACTAGACCACGGTGTACATGTTCGAGAGTGACTTTGGAATTGCCTAACTTGACTTTTACGATGATCCACATGACTAAACTACTTTTGGCCATAGATTCATTGAAGAGTTTGGAGGTGATTTTGAAAGATGTTTAGAAAATTAGAGAGAGGAGCCCCGTGGTTGGACGAGCCTCCATGTTAATGTAAAGATTAAGGAGTAAATATATATTAGATCCGTATTATACCATTCACATGATATCAAAATGAATGTAACGTCTTTCTAATATATTTGTCTATGCTTTTTCATGAAGTTTAAATCTCTATTACTACCGTTGTAGTACTAAAATTTGGCTCTAATATCAATTTTTAGATTTTTGTAGAGTGATTCGAATAAC

mRNA sequence

CAGGCCGCGCGCCGCCCGTTGTGTTTGGTACCAAATTTTGATATCAACAGGCGGAGAGAGAGAAAGAAATTGATATCCATTTGTTAATTGGCCTAATCCATTGGACACCATTCATAGCTTCTTTCCAAGATCATAAAAACGAAATTTCCATCTCGATTCTCGATTCATTCTTCTCTCATAATTTTCCCATCAATCCTCGTCATCACCTTCTTCTTCGCCACAAATCCCTCTCTCTCTCTCTCTTCTCTGTACTTTTTTTTCTCTCTCTCCTAGTCCGCACTCTGTCATCACCTCCCCCAGTTTTCTTCCTCCATAAATATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAATTGACTAGACCACGGTGTACATGTTCGAGAGTGACTTTGGAATTGCCTAACTTGACTTTTACGATGATCCACATGACTAAACTACTTTTGGCCATAGATTCATTGAAGAGTTTGGAGGTGATTTTGAAAGATGTTTAGAAAATTAGAGAGAGGAGCCCCGTGGTTGGACGAGCCTCCATGTTAATGTAAAGATTAAGGAGTAAATATATATTAGATCCGTATTATACCATTCACATGATATCAAAATGAATGTAACGTCTTTCTAATATATTTGTCTATGCTTTTTCATGAAGTTTAAATCTCTATTACTACCGTTGTAGTACTAAAATTTGGCTCTAATATCAATTTTTAGATTTTTGTAGAGTGATTCGAATAAC

Coding sequence (CDS)

ATGGCTTTCTTTCAACCCCTTCTTACTCCGTCCGTTCATTGTTCATTAACTCTTTTTCTCTTTTTCCTTGTCGTCGTACCATTGCTTCCTCCCGCCGCTCCCATGCCTTTGCACGAAGTTTACTCTCCTCGCTTGGCCTATAGGGGTCCGGTTATTCCGGTCAGCAATCTCGTCAAGTTTCTCGAGCGTCTTCAGGTTGTGGCGCTCAATTCCTTTGGTAAACTTGATTTCGATCTTAAATATTACGTCGATTTGTCGCTTAAATTTGATTTGAATTCCACTGAGAGGGCTTTTGATGCCCTTTCACGATCCTCCAATGGCTCTGTCCCGGTCGAGGATTTGAAGTCGTTTATTAGCGAGTATTTCGATAGTGCTGGAACCGATATGGTGTACTCCGACCCTCTGGATTTTGTCCCTCGGCCTCAAGGGTTTTTGCCGAAGGTGGAGAATGCGGGGGTTAGAGCTTGGGCTTTGGAGATTCATAATTACTGGAAGAATCTTAGTCGGAAAGTCTCCGATGATATCATTCATCGCCCCGATGCTCACACTCTGCTGCCGTTGCCTATGCCCTGTATCATTCCCGGAGCCAGATTCCGGGAAGTTTACTATTGGGATTCCTATTGGATTATCAGAGGATTGTTAGCTAGTAAAATGTATGATACTGCCAAGGGAATTGTTATCAATCTCATTTCAATGATTGATGAATTTGGCCATGTTTTGAATGGTGCTAGAGCTTATTACACTAACAGAAGTCAGCCTCCTCTCTTGAGTTCCATGGTTTATGACATATACCTTAGAACAGGTGATTTAGAGTTCGTGAGGAATTCCCTCCCAGCTTTGATCAAAGAGCATATGTTCTGGAATTCAGGATTCCATTCTGTTACCGTCCGGAAGGCTCCTGGTGGAAATCATTCTTTGTCTAGATATTATGCAATGTGGAATGAACCCAGGCCTGAATCTTCTCTGCTGGATGAGAAACTTGCCTCAAAGTTTGTGAATAACTACGACAAGCAGCGTCTATACCGTGAACTCGCATCGGCTGCCGAATCTGGTTGGGATTTCAGTTCAAGATGGATGAGGGATCACACGGACTTATCAACATTGGCTACAACTTCAATCTTGCCAGTTGATCTCAATGTATTCATACTCAAGATGGAACTTGACATCTCCAATTTGGCAAGAGCTGCTGGAGATTTCTGCCTTGCTTATAAATTTTTGGAGGCTTCACTAGTCAGAAAGAAGACAATCAACTCTATTTTCTGGAACTCAGAGAAGGGACAATGGCTTGATTACTGGCTTGATAATGGCTCTCACAAGGGTGTTCATACATGGGATGCTCAGAACCAGAACAAGAACATATATGCTTCGAACTTCATTCCGCTCTGGATCGAATCGTTCTACAACGACAGCATACAGATGAAGAAAGTGTTGAAAAGCTTACGGAAGTCAGGCTTGCTATGCAATGCCGGGATTGCAACTTCTACTATTAATTCAGGAGAACAATGGGATTTCCCGAATGGTTGGGCGCCAATTCAGCACATGATCATCGAGGGACTGGCGAGATCGGAGTTGAAAGAAGCAAGGGTATTGGCTGAGGATATGGCCGTGAGATGGCTTCGAACCAACTATGTGGCTTACAAGAAAACAGGGTATATGCATGAGAAATATGATGTCCAAAAGTGTGGAGACTTCGGCGCAGGAGGTGAATATGTCCCTCAGACTGGTTTTGGATGGTCAAATGGAGTTGTTTTGGCATTCCTTGAAGAGTTTGGATGGCCCGAAGATCAAAATATTGACTGCTAA

Protein sequence

MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Homology
BLAST of CmoCh02G009180 vs. ExPASy Swiss-Prot
Match: Q9SU50 (Trehalase OS=Arabidopsis thaliana OX=3702 GN=TRE1 PE=2 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 3.9e-193
Identity = 317/555 (57.12%), Postives = 417/555 (75.14%), Query Frame = 0

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +P+DFV  P GFL  VEN  VR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + + +GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +   TW A+NQN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625

BLAST of CmoCh02G009180 vs. ExPASy Swiss-Prot
Match: Q9FWC1 (Probable trehalase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 3.2e-187
Identity = 316/567 (55.73%), Postives = 413/567 (72.84%), Query Frame = 0

Query: 42  SPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDAL 101
           +P  A  G  +    L+  L+R+Q  AL +FG  DFD K YVDL L  D +    A  AL
Sbjct: 2   APTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADAS----AAAAL 61

Query: 102 SRSSNGSVPVEDLKSFISEYFDSAGTDMV-YSDPLDFVPRPQGFLPKVENAGVRAWALEI 161
           +     +    +++++IS YF  AG+D+V  +DP DF   P GFLP+VE A  RAWALE+
Sbjct: 62  ASLPRAAPSRGEMEAYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALEV 121

Query: 162 HNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYD 221
           H  WK+L+R+V+  +  RPD HTLLPLP   ++PG+RFREVYYWDSYW++RGLL SKMY+
Sbjct: 122 HALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMYE 181

Query: 222 TAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPAL 281
           TAK IV+NL+ +++++G VLNGAR+YYTNRSQPPLLSSMV DIY+ TGD+ FVR   P+L
Sbjct: 182 TAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPSL 241

Query: 282 IKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRL 341
           +KEH FW S  H+V V    G  H+LSRY AMWN+PRPES+ +DE+ ASK ++   K++ 
Sbjct: 242 LKEHSFWMSEVHNVAVMDNHGRVHNLSRYQAMWNKPRPESATIDEEFASK-LSTAAKEKF 301

Query: 342 YRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFC 401
           Y ++AS AE+GWDFSSRWMRD TD++TL T+ I+PVDLN FILKME DI+  A+  G+  
Sbjct: 302 YHQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLIGEST 361

Query: 402 LAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL-DNGSHKGVHTWDAQNQNKNIYASNFI 461
            +  F EAS  R   I+S+ WN++  QWLDYWL  +G+ +GV+ W + +QN+ I+ASNF+
Sbjct: 362 TSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFASNFV 421

Query: 462 PLWIESFYN------DSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQH 521
           PLW+ + ++      D  +  +V++SL+KSGLL  AGIATS  N+G+QWDFPNGWAP+QH
Sbjct: 422 PLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQWDFPNGWAPLQH 481

Query: 522 MIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQ 581
           +I+EGL RS   EAR LAED+A RW+RTNY AYK TG MHEKYDV  CG  G GGEY PQ
Sbjct: 482 LIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGGGEYKPQ 541

Query: 582 TGFGWSNGVVLAFLEEFGWPEDQNIDC 601
           TGFGWSNGV+L+FL+EFGWP+D+ IDC
Sbjct: 542 TGFGWSNGVILSFLDEFGWPQDKKIDC 563

BLAST of CmoCh02G009180 vs. ExPASy Swiss-Prot
Match: O43280 (Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 4.2e-107
Identity = 207/523 (39.58%), Postives = 301/523 (57.55%), Query Frame = 0

Query: 74  KLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSD 133
           KL  D K +VD+ L        + F  LSR  N S+P E L++F+ E+F + G ++    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 134 PLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCII 193
           P D+   PQ FL K+ +A +RAWA ++H  WK L +K+  +++  P+  +L+    P I+
Sbjct: 106 PADWKDSPQ-FLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 194 PGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQP 253
           PG RF E YYWDSYW++ GLL S+M +T KG++ N + ++  +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 254 PLLSSMVYDIYL-RTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAM 313
           PLL+ M+ D YL  T D  F++ ++  L  E  FW     +V+V    G N+ L+RYY  
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTKN-RTVSV-SLEGKNYLLNRYYVP 285

Query: 314 WNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLA 373
           +  PRPES   D +LA       D++ L+ EL + AESGWDFSSRW+    +   LS + 
Sbjct: 286 YGGPRPESYSKDVELADTLPEG-DREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIR 345

Query: 374 TTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWL 433
           T+ ++PVDLN F+ + E  +SN     G+   A K+      R   +N++ W+ + G W 
Sbjct: 346 TSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWF 405

Query: 434 DYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-N 493
           DY L+            + +N+  Y SN  PLW   F +  +   K LK L  + +L   
Sbjct: 406 DYDLEK-----------KKKNREFYPSNLTPLWAGCFSDPGV-ADKALKYLEDNRILTYQ 465

Query: 494 AGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKK 553
            GI TS   +G+QWDFPN WAP+Q ++I GLA++ L+ A+ +A  +A  W+RTN+  Y +
Sbjct: 466 YGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQ 525

Query: 554 TGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
              M+EKYDV   G  G GGEY  Q GFGW+NGVVL  L+ +G
Sbjct: 526 KSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYG 551

BLAST of CmoCh02G009180 vs. ExPASy Swiss-Prot
Match: Q9JLT2 (Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.3e-103
Identity = 199/519 (38.34%), Postives = 291/519 (56.07%), Query Frame = 0

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDF 137
           D K +VD+SL    +   + F  L+   N S+P E L+ F+  +F   G ++    P D+
Sbjct: 47  DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDW 106

Query: 138 VPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ +A +R WA E+H  WK L +K+  +++  P+  +L+    P I+PG R
Sbjct: 107 KDSPQ-FLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSYPERSSLIYSKHPFIVPGGR 166

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M  T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 167 FVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLT 226

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGG-NHSLSRYYAMWNEP 317
            M+      T D+ F++ ++  L  E  FW     + TV    GG ++ L+RYY  +  P
Sbjct: 227 LMMDRYVAHTKDVAFLQENIGTLASELDFWTV---NRTVSVVSGGQSYVLNRYYVPYGGP 286

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTD---LSTLATTSI 377
           RPES   D +LA+  V   D++ L+ EL + AESGWDFSSRW+    D   LS++ T+ +
Sbjct: 287 RPESYRKDAELANS-VPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKM 346

Query: 378 LPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +P DLN F+ + E  +SN     G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 347 VPADLNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDL 406

Query: 438 DNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC-NAGIA 497
           + G            +N   Y SN  PLW   F + S+   K LK L  S +L    GI 
Sbjct: 407 EKG-----------KKNLEFYPSNLSPLWAGCFSDPSV-ADKALKYLEDSKILTYQYGIP 466

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W++TN+  Y +   M
Sbjct: 467 TSLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAM 526

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
            EKYD+   G  G GGEY  Q GFGW+NG+ L  L+ +G
Sbjct: 527 FEKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYG 548

BLAST of CmoCh02G009180 vs. ExPASy Swiss-Prot
Match: P19813 (Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.7e-103
Identity = 198/519 (38.15%), Postives = 297/519 (57.23%), Query Frame = 0

Query: 78  DLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDF 137
           D K +VD+ L    +   ++F  L+ + N +VP E L+ F+ E+F + G ++    P D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 138 VPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGAR 197
              PQ FL K+ +  +RAWA ++H  WK L +K+  +++ +P+  +L+    P I+PG R
Sbjct: 110 KESPQ-FLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 198 FREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLS 257
           F E YYWDSYW++ GLL S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 258 SMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGN-HSLSRYYAMWNEP 317
            M+      TGDL F+R ++  L  E  FW     + T+  + GGN H+L+RY+  +  P
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFW---AENRTISVSSGGNSHTLNRYHVPYGGP 289

Query: 318 RPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWM---RDHTDLSTLATTSI 377
           RPES   D +LA         + L+ EL + AESGWDFSSRW+    +   L ++ T+ +
Sbjct: 290 RPESYSKDTELAHTLPEG-SWETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSKL 349

Query: 378 LPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWL 437
           +PVDLN F+ + E  +S      G+   A K+      R   + ++ W+ +KG W DY L
Sbjct: 350 VPVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYDL 409

Query: 438 DNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNA-GIA 497
           +N           Q +N   Y SN  PLW   F + +I   K L+ L+ S +L +  GI 
Sbjct: 410 EN-----------QKKNHEFYPSNLTPLWAGCFSDPAI-ADKALQYLQDSQILNHRHGIP 469

Query: 498 TSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYM 557
           TS  N+G+QWDFPN WAP+Q ++I GLA+S     + +A  +A  W+RTN+  Y +   M
Sbjct: 470 TSLQNTGQQWDFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRSAM 529

Query: 558 HEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFG 592
           +EKYD+      G GGEY  Q GFGW+NGV L  L+ +G
Sbjct: 530 YEKYDISNAQP-GGGGEYEVQEGFGWTNGVALMLLDRYG 550

BLAST of CmoCh02G009180 vs. ExPASy TrEMBL
Match: A0A6J1H564 (Trehalase OS=Cucurbita moschata OX=3662 GN=LOC111459683 PE=3 SV=1)

HSP 1 Score: 1240.7 bits (3209), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of CmoCh02G009180 vs. ExPASy TrEMBL
Match: A0A6J1K2W1 (Trehalase OS=Cucurbita maxima OX=3661 GN=LOC111491217 PE=3 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 585/600 (97.50%), Postives = 591/600 (98.50%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTP  HCSLTLFLFFL VVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPCHCSLTLFLFFL-VVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLK+FI E
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKTFIGE 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDP+DFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPC+IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCVIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHTFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLY ELAS AESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYSELASTAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARA GDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFY+DSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYSDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATSTINSGEQWDFPNGWAPIQHMI+EGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RNSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIVEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 599

BLAST of CmoCh02G009180 vs. ExPASy TrEMBL
Match: A0A1S3ATK4 (Trehalase OS=Cucumis melo OX=3656 GN=LOC103482823 PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 4.0e-302
Identity = 504/600 (84.00%), Postives = 543/600 (90.50%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           M    P L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKF
Sbjct: 1   MPVLHPHLPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS 
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISN 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YF SAGTD+VYS+P+DF P+P GFLPKVEN  VR WAL+IHN+WKNLSR+VSDD+IH PD
Sbjct: 121 YFHSAGTDLVYSNPVDFNPQPDGFLPKVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPLP P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 THTLLPLPEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGAR+YYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHSVTV+   
Sbjct: 241 NGARSYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG- 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
            GNHSLSRYYAMWNEPRPESSL+DEKLASKF NNY+K+ LYRE+ASAAESGWDFSSRWMR
Sbjct: 301 NGNHSLSRYYAMWNEPRPESSLVDEKLASKFANNYEKKHLYREIASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           D TDLSTLATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINSIF
Sbjct: 361 DSTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGS+KG H+W+A+NQN+NIYASNFIPLWIESFY+DS QMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSYKGAHSWNARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLR
Sbjct: 481 RNSGLLCNAGIATSVINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYK TG+MHEKYDVQKCGDFG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 541 TNYVAYKHTGFMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of CmoCh02G009180 vs. ExPASy TrEMBL
Match: A0A0A0KMF2 (Trehalase OS=Cucumis sativus OX=3659 GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 9.9e-301
Identity = 498/593 (83.98%), Postives = 539/593 (90.89%), Query Frame = 0

Query: 8   LTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVV 67
           L P    SLTLF FFL++  L P AA +P  EV+SPRLA +GPVIPV NLVKFLER+QVV
Sbjct: 8   LPPIRFSSLTLFHFFLIMPLLRPAAASLP--EVFSPRLADKGPVIPVPNLVKFLERIQVV 67

Query: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGT 127
           ALNSFGKLDFDLKYYVDLSLKFDLNST+ AFDAL RSSNGSV VE+L++FIS YFDSAGT
Sbjct: 68  ALNSFGKLDFDLKYYVDLSLKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGT 127

Query: 128 DMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPL 187
           D+VYS+P DF P P GFLPKVEN  VRAWA +IHN+WKNLSR+VSDD+IH PD HTLLPL
Sbjct: 128 DLVYSNPADFDPHPDGFLPKVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPL 187

Query: 188 PMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYY 247
           P P ++PG+RFRE+YYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YY
Sbjct: 188 PEPVVVPGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYY 247

Query: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLS 307
           TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH FWNSGFHS+TV+    GNHSLS
Sbjct: 248 TNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLS 307

Query: 308 RYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLST 367
           RYYAMWNEPRPESSL+DEK+ASKFVNNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLST
Sbjct: 308 RYYAMWNEPRPESSLVDEKVASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLST 367

Query: 368 LATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQ 427
           LATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINSIFWNSEKGQ
Sbjct: 368 LATTSILPVDLNVFILKMELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQ 427

Query: 428 WLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLC 487
           WLDYWLDNGS+KG H+WD +NQN+N+YASNFIPLW+ESFY+DS QMKKVLKSLR SGLLC
Sbjct: 428 WLDYWLDNGSYKGAHSWDVRNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLC 487

Query: 488 NAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYK 547
           NAGIATS INSGEQWDFPNGWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK
Sbjct: 488 NAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYK 547

Query: 548 KTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
            TG+MHEKYDVQKCG FG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 548 HTGFMHEKYDVQKCGGFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 597

BLAST of CmoCh02G009180 vs. ExPASy TrEMBL
Match: A0A5A7TH01 (Trehalase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001860 PE=3 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 9.3e-299
Identity = 494/575 (85.91%), Postives = 531/575 (92.35%), Query Frame = 0

Query: 26  VPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDL 85
           +PLL PAA   L EV+SPRLA +GPVIPV NLVKFLERLQVVALNSFGKLDFDLKYYVDL
Sbjct: 1   MPLLRPAA-ASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDL 60

Query: 86  SLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFL 145
           SLKFDLNST+ AFDAL RSSNGSV VE+L++FIS YF SAGTD+VYS+P+DF P+P GFL
Sbjct: 61  SLKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFL 120

Query: 146 PKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWD 205
           PKVEN  VR WAL+IHN+WKNLSR+VSDD+IH PD HTLLPLP P ++PG+RFRE+YYWD
Sbjct: 121 PKVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWD 180

Query: 206 SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYL 265
           SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGAR+YYTNRSQPPLLSSMVYDIYL
Sbjct: 181 SYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYL 240

Query: 266 RTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDE 325
           RTGDLEFVRNSLPALIKEH FWNSGFHSVTV+    GNHSLSRYYAMWNEPRPESSL+DE
Sbjct: 241 RTGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG-NGNHSLSRYYAMWNEPRPESSLVDE 300

Query: 326 KLASKFVNNYDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKM 385
           KLASKF NNY+K+ LYRE+ASAAESGWDFSSRWMRD TDLSTLATTSILPVDLNVFILKM
Sbjct: 301 KLASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKM 360

Query: 386 ELDISNLARAAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWD 445
           ELDISNLARA GD+C A  F EASLVRKKTINSIFWNSEKGQWLDYWLDNGS+KG H+W+
Sbjct: 361 ELDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWN 420

Query: 446 AQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFP 505
           A+NQN+NIYASNFIPLWIESFY+DS QMKKVLKSLR SGLLCNAGIATS INSGEQWDFP
Sbjct: 421 ARNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDFP 480

Query: 506 NGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFG 565
           NGWAPIQHMI+EGLARSEL EA+ LAED+A RWLRTNYVAYK TG+MHEKYDVQKCGDFG
Sbjct: 481 NGWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDFG 540

Query: 566 AGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 601
            GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQ IDC
Sbjct: 541 GGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDC 573

BLAST of CmoCh02G009180 vs. NCBI nr
Match: XP_022958464.1 (probable trehalase [Cucurbita moschata])

HSP 1 Score: 1240.7 bits (3209), Expect = 0.0e+00
Identity = 600/600 (100.00%), Postives = 600/600 (100.00%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of CmoCh02G009180 vs. NCBI nr
Match: XP_023534407.1 (probable trehalase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 593/600 (98.83%), Postives = 596/600 (99.33%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTP VHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDP+DFVPRPQGFLPKVENA VRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAEVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARA GDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVH WDA++QNKNIYASNFIPLWIESFYNDSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHAWDARSQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600

BLAST of CmoCh02G009180 vs. NCBI nr
Match: XP_022995786.1 (probable trehalase [Cucurbita maxima])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 585/600 (97.50%), Postives = 591/600 (98.50%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQPLLTP  HCSLTLFLFFL VVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF
Sbjct: 1   MAFFQPLLTPPCHCSLTLFLFFL-VVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLK+FI E
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKTFIGE 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTDMVYSDP+DFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD
Sbjct: 121 YFDSAGTDMVYSDPVDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
           AHTLLPLPMPC+IPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 AHTLLPLPMPCVIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHSVTVRKAP
Sbjct: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHTFWNSGFHSVTVRKAP 300

Query: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRWMR 360
           GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLY ELAS AESGWDFSSRWMR
Sbjct: 301 GGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYSELASTAESGWDFSSRWMR 360

Query: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINSIF 420
           DHTDLSTLATTSILPVDLNVFILKMELDISNLARA GDFCLAYKFLEASLVRKKTINSIF
Sbjct: 361 DHTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDFCLAYKFLEASLVRKKTINSIF 420

Query: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLKSL 480
           WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFY+DSIQMKKVLKSL
Sbjct: 421 WNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYSDSIQMKKVLKSL 480

Query: 481 RKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRWLR 540
           R SGLLCNAGIATSTINSGEQWDFPNGWAPIQHMI+EGLARSELKEARVLAEDMAVRWLR
Sbjct: 481 RNSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIVEGLARSELKEARVLAEDMAVRWLR 540

Query: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 600
           TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 TNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNIDC 599

BLAST of CmoCh02G009180 vs. NCBI nr
Match: KAG6605573.1 (Trehalase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 564/566 (99.65%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 35  MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST 94
           MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST
Sbjct: 1   MPLHEVYSPRLAYRGPVIPVSNLVKFLERLQVVALNSFGKLDFDLKYYVDLSLKFDLNST 60

Query: 95  ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVR 154
           ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVR
Sbjct: 61  ERAFDALSRSSNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVR 120

Query: 155 AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL 214
           AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL
Sbjct: 121 AWALEIHNYWKNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLL 180

Query: 215 ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR 274
           ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR
Sbjct: 181 ASKMYDTAKGIVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVR 240

Query: 275 NSLPALIKEHMFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN 334
           NSLPALIKEH+FWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN
Sbjct: 241 NSLPALIKEHVFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNN 300

Query: 335 YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR 394
           YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR
Sbjct: 301 YDKQRLYRELASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLAR 360

Query: 395 AAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIY 454
           AAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIY
Sbjct: 361 AAGDFCLAYKFLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIY 420

Query: 455 ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM 514
           ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM
Sbjct: 421 ASNFIPLWIESFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHM 480

Query: 515 IIEGLARSELKEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQT 574
           IIEGLARSELKEARVLAEDMAVRWLRTNYVAY+KTGYMHEKYDVQKCGDFGAGGEYVPQT
Sbjct: 481 IIEGLARSELKEARVLAEDMAVRWLRTNYVAYEKTGYMHEKYDVQKCGDFGAGGEYVPQT 540

Query: 575 GFGWSNGVVLAFLEEFGWPEDQNIDC 601
           GFGWSNGVVLAFLEEFGWPEDQNIDC
Sbjct: 541 GFGWSNGVVLAFLEEFGWPEDQNIDC 566

BLAST of CmoCh02G009180 vs. NCBI nr
Match: XP_038907056.1 (probable trehalase [Benincasa hispida] >XP_038907058.1 probable trehalase [Benincasa hispida])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.5e-309
Identity = 514/602 (85.38%), Postives = 553/602 (91.86%), Query Frame = 0

Query: 1   MAFFQPLLTPSVHCSLTLFLFFLVVVPLLPPAAPMPLHEVYSPRLAYRGPVIPVSNLVKF 60
           MAFFQP   P     LTLF  FL++  L P  A +P  EV+SPRL  RGPV+PV+NLVKF
Sbjct: 1   MAFFQPHFPPIPFSPLTLFHLFLIMPLLRPATATLP--EVFSPRLVDRGPVVPVTNLVKF 60

Query: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLNSTERAFDALSRSSNGSVPVEDLKSFISE 120
           LERLQVVALNSFGKLDFDLKYYVDLSLKFDL+STE AFDAL RSSNGSV VE+LK+FI++
Sbjct: 61  LERLQVVALNSFGKLDFDLKYYVDLSLKFDLDSTETAFDALERSSNGSVSVENLKTFITD 120

Query: 121 YFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYWKNLSRKVSDDIIHRPD 180
           YFDSAGTD+VYS+P+DFVP+P GFLPKVEN+ VRAWAL+IHN+WKNLSR+VSDD+IHRP+
Sbjct: 121 YFDSAGTDLVYSEPVDFVPQPIGFLPKVENSEVRAWALDIHNFWKNLSRRVSDDLIHRPE 180

Query: 181 AHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240
            HTLLPLP P ++PG+RFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL
Sbjct: 181 THTLLPLPEPVVVPGSRFREVYYWDSYWIIRGLLASKMYDTAKGIVINLISMIDEFGHVL 240

Query: 241 NGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEHMFWNSGFHSVTVRKAP 300
           NGAR+YYTNRSQPPLLSSMVYDIYLRTGDLEFVRN+LPALIKEH FWNSGFHS+TVR AP
Sbjct: 241 NGARSYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNALPALIKEHKFWNSGFHSITVRNAP 300

Query: 301 G--GNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYRELASAAESGWDFSSRW 360
           G  GNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNY+KQ LYRE+ASAAESGWDFSSRW
Sbjct: 301 GGNGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYEKQHLYREIASAAESGWDFSSRW 360

Query: 361 MRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYKFLEASLVRKKTINS 420
           MRD TDLSTLATTSILPVDLNVFILKMELDISNLARA GD+C A  F EASLVRKKTINS
Sbjct: 361 MRDSTDLSTLATTSILPVDLNVFILKMELDISNLARAVGDYCTAKHFFEASLVRKKTINS 420

Query: 421 IFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIESFYNDSIQMKKVLK 480
           IFWNSEKGQWLDYWLDNG +KG HTWDA+NQN+N+YASNFIPLWIESFY+DS QMKKVLK
Sbjct: 421 IFWNSEKGQWLDYWLDNGLYKGTHTWDARNQNQNVYASNFIPLWIESFYSDSRQMKKVLK 480

Query: 481 SLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSELKEARVLAEDMAVRW 540
           SLR SGLLCNAGIATS INSGEQWDFPNGWAPIQHMI+EGLARS LKEAR LAED+A RW
Sbjct: 481 SLRNSGLLCNAGIATSMINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIAARW 540

Query: 541 LRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVLAFLEEFGWPEDQNI 600
           L+TNYVAYK TGYMHEKYDVQKCGDFG GGEYVPQTGFGWSNGVVLAFLEEFGWP+DQNI
Sbjct: 541 LQTNYVAYKHTGYMHEKYDVQKCGDFGGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQNI 600

BLAST of CmoCh02G009180 vs. TAIR 10
Match: AT4G24040.1 (trehalase 1 )

HSP 1 Score: 676.0 bits (1743), Expect = 2.8e-194
Identity = 317/555 (57.12%), Postives = 417/555 (75.14%), Query Frame = 0

Query: 49  GPVIPVSNLVKFLERLQVVALNSFGKLDF-DLKYYVDLSLK--FDLNSTERAFDAL-SRS 108
           GPV+  + LV FL+R+Q  AL S+ K    D K Y+DLSLK  + L++ E AFD L S S
Sbjct: 72  GPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTSES 131

Query: 109 SNGSVPVEDLKSFISEYFDSAGTDMVYSDPLDFVPRPQGFLPKVENAGVRAWALEIHNYW 168
            +  VPVE L+ F+ EYFD AG D+++ +P+DFV  P GFL  VEN  VR WA E+H  W
Sbjct: 132 HDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHGLW 191

Query: 169 KNLSRKVSDDIIHRPDAHTLLPLPMPCIIPGARFREVYYWDSYWIIRGLLASKMYDTAKG 228
           +NLS +VSD +    D HTLLPLP P IIPG+RFREVYYWDSYW+I+GL+ S+M+ TAKG
Sbjct: 192 RNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTAKG 251

Query: 229 IVINLISMIDEFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTGDLEFVRNSLPALIKEH 288
           +V NL+S+++ +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T D E VR ++P L+KE+
Sbjct: 252 LVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLKEY 311

Query: 289 MFWNSGFHSVTVRKAPGGNHSLSRYYAMWNEPRPESSLLDEKLASKFVNNYDKQRLYREL 348
            FWNSG H V +R A G +H LSRYYAMWN+PRPESS+ DE+ AS F    +KQR +R++
Sbjct: 312 EFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHRDI 371

Query: 349 ASAAESGWDFSSRWMRDHTDLSTLATTSILPVDLNVFILKMELDISNLARAAGDFCLAYK 408
           A+AAESG DFS+RWMRD  + +T+ATTS++PVDLNVF+LKMELDI+ + + +GD   + +
Sbjct: 372 ATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGSDR 431

Query: 409 FLEASLVRKKTINSIFWNSEKGQWLDYWLDNGSHKGVHTWDAQNQNKNIYASNFIPLWIE 468
           F++AS  R+K   ++FWN + GQWLDYWL +   +   TW A+NQN N++ASNF P+WI 
Sbjct: 432 FVKASKAREKAFQTVFWNEKAGQWLDYWLSSSGEES-ETWKAENQNTNVFASNFAPIWIN 491

Query: 469 SFYNDSIQMKKVLKSLRKSGLLCNAGIATSTINSGEQWDFPNGWAPIQHMIIEGLARSEL 528
           S  +D   +KKV+ +L+ SGL+  AGI TS  NSG+QWD PNGWAP Q MI+ GL RS +
Sbjct: 492 SINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRSSV 551

Query: 529 KEARVLAEDMAVRWLRTNYVAYKKTGYMHEKYDVQKCGDFGAGGEYVPQTGFGWSNGVVL 588
           KEA+ +AED+A RW+++NY+ YKK+G +HEK  V + G++G GGEY+PQTGFGWSNGV+L
Sbjct: 552 KEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGVIL 611

Query: 589 AFLEEFGWPEDQNID 600
           AFLEE+GWP   +I+
Sbjct: 612 AFLEEYGWPSHLSIE 625

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SU503.9e-19357.12Trehalase OS=Arabidopsis thaliana OX=3702 GN=TRE1 PE=2 SV=1[more]
Q9FWC13.2e-18755.73Probable trehalase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0521000 PE=2... [more]
O432804.2e-10739.58Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2[more]
Q9JLT21.3e-10338.34Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1[more]
P198131.7e-10338.15Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H5640.0e+00100.00Trehalase OS=Cucurbita moschata OX=3662 GN=LOC111459683 PE=3 SV=1[more]
A0A6J1K2W10.0e+0097.50Trehalase OS=Cucurbita maxima OX=3661 GN=LOC111491217 PE=3 SV=1[more]
A0A1S3ATK44.0e-30284.00Trehalase OS=Cucumis melo OX=3656 GN=LOC103482823 PE=3 SV=1[more]
A0A0A0KMF29.9e-30183.98Trehalase OS=Cucumis sativus OX=3659 GN=Csa_5G149880 PE=3 SV=1[more]
A0A5A7TH019.3e-29985.91Trehalase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001860 PE=... [more]
Match NameE-valueIdentityDescription
XP_022958464.10.0e+00100.00probable trehalase [Cucurbita moschata][more]
XP_023534407.10.0e+0098.83probable trehalase [Cucurbita pepo subsp. pepo][more]
XP_022995786.10.0e+0097.50probable trehalase [Cucurbita maxima][more]
KAG6605573.10.0e+0099.65Trehalase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038907056.15.5e-30985.38probable trehalase [Benincasa hispida] >XP_038907058.1 probable trehalase [Benin... [more]
Match NameE-valueIdentityDescription
AT4G24040.12.8e-19457.12trehalase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001661Glycoside hydrolase, family 37PRINTSPR00744GLHYDRLASE37coord: 416..432
score: 36.22
coord: 574..587
score: 60.15
coord: 373..390
score: 42.4
coord: 236..254
score: 62.6
coord: 341..358
score: 55.85
coord: 306..323
score: 40.64
coord: 494..510
score: 60.06
IPR001661Glycoside hydrolase, family 37PFAMPF01204Trehalasecoord: 75..591
e-value: 2.0E-167
score: 558.1
IPR001661Glycoside hydrolase, family 37PANTHERPTHR23403TREHALASEcoord: 64..600
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 63..599
e-value: 2.8E-173
score: 579.1
NoneNo IPR availablePANTHERPTHR23403:SF1TREHALASEcoord: 64..600
IPR018232Glycoside hydrolase, family 37, conserved sitePROSITEPS00928TREHALASE_2coord: 501..510
IPR008928Six-hairpin glycosidase superfamilySUPERFAMILY48208Six-hairpin glycosidasescoord: 72..593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G009180.1CmoCh02G009180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005991 trehalose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004555 alpha,alpha-trehalase activity
molecular_function GO:0005509 calcium ion binding