CmoCh02G009130 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G009130
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectin lyase-like superfamily protein
LocationCmo_Chr02: 5617143 .. 5620933 (-)
RNA-Seq ExpressionCmoCh02G009130
SyntenyCmoCh02G009130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGCCGAGCTCTGATTCTCTGGTTACTGCTCTCTCTCTCTCTAACCCTTTCCATTAATTAACTTCTCCATTTCGCACCATCCTTGGATTCTTCTTCGTGTCCTAACTCCCAGATCTACTCCCCCTTCTTCCCATCTTCGTCTTCTTCTTTAAGATCTTACTTAAATCCCTCAAAAAGCGTCTCTCCACTCTTTTCTCTTTGTTCTCTTTCCCATTTCTCCACCATGTTGCCGTTTTTCCTCTTCATTTTCATCTGCTTCGCTGCTCTTTTGTCCTCTCCACTTGCCGCCGGTGAGCTCACTACCTGCTCCGGGATAGTCCCCATGAAGTATCGGAGCGATAAGATTTCCATTGCCGATTTTGGGGGTGTCGGCGATGGCCGTACATTGAACACTAGAGCTTTTCGAGCTGCGATTTATCGAATTCAACATTTGAGAAGAAGAGGGGGTACTTTGCTTTACATTCCTCCTGGGGTTTACTTGACGGAGAGCTTTAATCTCACTAGCCATATGACTCTGTATCTTGCAAAAGACGCTGTTATCAAAGCTGTTCAGGTATATGCTTCTCACTGAACTTCTTTGTTGTTTCTCTTATTTGTGATTCAGTAGTGTGGCTAGTGTTGGTTGTTGTTGAATGAGAGAATTTCCCTGATTTTTCGCTGGTGATTCTCGTGCTTAGGGTTTCTTTCGTTTGGTGATTCTGATTTTGAGCTTATTTTTGTCGGTGATTATGAATGGTTTCTAGATTAGGGGATTGGGATTTATCAGAATGAACGTGTGCTCTGGTGGGTGGGTATTCGATTAAGCTGTGGCATGTGCATCCACTCTAAATTTATAACATTATATATAATTATGATTGGACCGTCACAGGCTGTTCTTTGTCGGATTCTGATGCTAAATTTTTATTAGAATGTGATCCCTTTTTAGTTGATTGATCCATTCAAAGTTCTGATTTGCTAATTTGTGATTATTATTTTGCTGATCTGGACATCAATTTCAAGCAACCAAAATTGGCTGTCCCATAGTTGAAATATCTATGTCAAAACATTGGTTGATTCTTTTAACAAGGATGATGACAATGTTATTAGTTAGTTGTAGCAATTAATTAACTCAATCCATGTCTATTTCAGCTAGCAAACAGGCCAGAAAGGATCTGTTTTAGGCAATCGGATCCTTTCCTTAATGTTCTTTTGTTTTTGTGAGATCCCACATCGGTTGGAGAAGAAAACAAAACCTTACTTATAAAGGTGTGGAAATCTCTCCCTAGAAGACGCGTTTTAAAATCGTGATGCTGACGACGATATGTAACAAGCCGAAGCAGACAATATCTGCTAGTGGTGGGCTTGGTCTCGTACAAATGGTATCAGAGCCAGGTTACCATGCGAGGCACTAGTTGGAGTAGGACTAGACTATCTTCATAGTAGACGCATTTTAAAACCATAAGGTTGACAGTGATATGTAATGAGCCAAAGCGGACAATATCTACTTGCGGTGGGCTCAGGCTATTACAGTTTTCCAAGAAGGAACTTTACCTATTTCTCTAGTACTTGAAGTAAAATGCGTGGAAATTATGTCAAGATTTTGAAACTAGAACTGTTATATGTTGTCGGTGTCTAAAATTTGACTCCTATATGATTACCCGCTTCCAATAAGCATTTTTTTTATTCTGGGTTTGTATGCATCCTAATGGATGGTCAATTTAGTGAGTGCAAGATCTTTGGTCTAAGAGTTTTCACCATTCGTATCCATCAACTTCTCGATGATGTAATTTTCTGCAGATGATGAGAATTGTCCGATCTTTTCATAAAATTTCTCTCTTTTTTTAAACTAGTTTAATCCTTCACTTTTATTGTTTTACTTTTGTATTAAGCTTTTGTTTTTTTTTCTCTGTTTGATGTCCAGGATTCTTCAAATTGGCCAGTGATCGAGCCGTTGCCATCATATGGAAGAGGGAGGGAGCGCCCTGGTGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTATGAATCATCTAGTTCTTTTAAAATGTATATAAAGGCTATTGCAAACCTGGTTTACTTGTGTTGTTCTTGGAACAAAAGTATGTATATTAATAGCCCGAGTTAAATTGTTGTGGTAGGTGAGAATGGGACCATTGATGGCCAAGGTGATGTCTGGTGGAATATGTGGAGGACAAGGACTCTGATGTACACTAGGCCTAATCTCGTTGAATTTATCAATTCTCATAATATCATCATTTCCAACGTAATGTTCCTGAACTCTCCGTTTTGGAACATTCATCCAGTTTACTGCAGGTAAGAATTAAAGTCCGTTTTCAATCACGTTACAGGTAAGAATTAAAGTCCGTTTTCAATCACGTTACAGCTCCATAATTGCTCATATCGTGTTGAAATACCCCTTGAATGTTCATCATATTTTCTGCTTTTCTTCCAACCAAAACACTTTTAACTTGTGAGATCCCACATTGGTTGGAGAGAGAAACGAAGCATTCTTTATAAGAGTATGAAAATCTCTCCTTAGTAGAGCGTTTTAAAATCATGAGGTTGATGACGATACGTAGCGGGGAAAAGCGGATAATATCTACTAGCGGTGAGCTTGGACTCTTACAAATGGTATCAAAGTTAGTCATCGAGCATTGTGCCAACGAGGACGTTGGGTTCCCGAGGGGGTGGATTGTGAGATCCCACCTTGGTTATGGTTGGAGAGTAGAACGAAGTATTCCTTATAAGGGTGTGGAAAACTCTCCCTAGCAGACGCGTTTTAAAACCGTGAGGTTGACGGTGATACATAATGGGCCAAAACGGACAATATTTACCAGGGTGGGCTTGAGCTATTAAACTCCTTTCAGGGTAATATCAACAGTGTGCGATCTTACTTTTTTTCTAATCTTCTGTTGCAGTAACGTTGTTGTGAGATATGTCACAATATTGGCTCCTCGTGATTCCCCGAACACCGATGGAATCGATCCAGGTGCGTTTTCAGATCCGTAGTTATTAGCTCCCCCACCCCCGCCCCTGGTCGACCTCTGGTTAAAATAATTGCTTATTACAAGTCATGTTCAAAGTTCTCATAACATTAGTTCTGTATATGCCTGAAAGTAACAAAACTTGTCAAACTGATGAACTCAGACTCGAGCAACAACGTCTGTATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGACGAATATGGTATCGCTTACGGTCGTAGTAGTTACGACATTACCATCCGAAGAATATCGGGGTCAAGTCCATTTGCTGGAGTTGCTGTAGGAAGCGAAACCTCCGGTGGTGTGTCAAATGTATTAGCAGAACACCTAAGCTTATACGACATGGGAGTTGGTATCAACATCAAGACAAACATTGGAAGAGGAGGGTTCATAAAGAACATAACCATATCCGATGTCTACATGGAGAGCTCGCGAAAGGGTCTAAAGATTGCGAGCGATACTGGAGATCATCCCGACGACAAGTTCGACCCAAACGCTCTTCCAATCGTCAAAGACATCACAATTAAAAACGTTTGGGGTGAGAATGTGCAGCAAGCAGGTTCTATATATGGCTTGAGGAACTCCCCATTTACTGGTATATGTCTTTCGAACATTAACCTTCGTGGCACAGCGAGACCGAGATCAGTACCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTCTTGGTTAGTCCATGGCCATGCACAGAGCTTACCAGCACACAGCAGGATGGTGTGTGCTCCAATAACTTCTAA

mRNA sequence

ACAGCCGAGCTCTGATTCTCTGGTTACTGCTCTCTCTCTCTCTAACCCTTTCCATTAATTAACTTCTCCATTTCGCACCATCCTTGGATTCTTCTTCGTGTCCTAACTCCCAGATCTACTCCCCCTTCTTCCCATCTTCGTCTTCTTCTTTAAGATCTTACTTAAATCCCTCAAAAAGCGTCTCTCCACTCTTTTCTCTTTGTTCTCTTTCCCATTTCTCCACCATGTTGCCGTTTTTCCTCTTCATTTTCATCTGCTTCGCTGCTCTTTTGTCCTCTCCACTTGCCGCCGGTGAGCTCACTACCTGCTCCGGGATAGTCCCCATGAAGTATCGGAGCGATAAGATTTCCATTGCCGATTTTGGGGGTGTCGGCGATGGCCGTACATTGAACACTAGAGCTTTTCGAGCTGCGATTTATCGAATTCAACATTTGAGAAGAAGAGGGGGTACTTTGCTTTACATTCCTCCTGGGGTTTACTTGACGGAGAGCTTTAATCTCACTAGCCATATGACTCTGTATCTTGCAAAAGACGCTGTTATCAAAGCTGTTCAGGATTCTTCAAATTGGCCAGTGATCGAGCCGTTGCCATCATATGGAAGAGGGAGGGAGCGCCCTGGTGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTGAGAATGGGACCATTGATGGCCAAGGTGATGTCTGGTGGAATATGTGGAGGACAAGGACTCTGATGTACACTAGGCCTAATCTCGTTGAATTTATCAATTCTCATAATATCATCATTTCCAACGTAATGTTCCTGAACTCTCCGTTTTGGAACATTCATCCAGTTTACTGCAGTAACGTTGTTGTGAGATATGTCACAATATTGGCTCCTCGTGATTCCCCGAACACCGATGGAATCGATCCAGACTCGAGCAACAACGTCTGTATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGACGAATATGGTATCGCTTACGGTCGTAGTAGTTACGACATTACCATCCGAAGAATATCGGGGTCAAGTCCATTTGCTGGAGTTGCTGTAGGAAGCGAAACCTCCGGTGGTGTGTCAAATGTATTAGCAGAACACCTAAGCTTATACGACATGGGAGTTGGTATCAACATCAAGACAAACATTGGAAGAGGAGGGTTCATAAAGAACATAACCATATCCGATGTCTACATGGAGAGCTCGCGAAAGGGTCTAAAGATTGCGAGCGATACTGGAGATCATCCCGACGACAAGTTCGACCCAAACGCTCTTCCAATCGTCAAAGACATCACAATTAAAAACGTTTGGGGTGAGAATGTGCAGCAAGCAGGTTCTATATATGGCTTGAGGAACTCCCCATTTACTGGTATATGTCTTTCGAACATTAACCTTCGTGGCACAGCGAGACCGAGATCAGTACCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTCTTGGTTAGTCCATGGCCATGCACAGAGCTTACCAGCACACAGCAGGATGGTGTGTGCTCCAATAACTTCTAA

Coding sequence (CDS)

ATGTTGCCGTTTTTCCTCTTCATTTTCATCTGCTTCGCTGCTCTTTTGTCCTCTCCACTTGCCGCCGGTGAGCTCACTACCTGCTCCGGGATAGTCCCCATGAAGTATCGGAGCGATAAGATTTCCATTGCCGATTTTGGGGGTGTCGGCGATGGCCGTACATTGAACACTAGAGCTTTTCGAGCTGCGATTTATCGAATTCAACATTTGAGAAGAAGAGGGGGTACTTTGCTTTACATTCCTCCTGGGGTTTACTTGACGGAGAGCTTTAATCTCACTAGCCATATGACTCTGTATCTTGCAAAAGACGCTGTTATCAAAGCTGTTCAGGATTCTTCAAATTGGCCAGTGATCGAGCCGTTGCCATCATATGGAAGAGGGAGGGAGCGCCCTGGTGGAAGATATGTAAGCTTTATTCATGGAGATGGTGTTCATGATGTGGTGATCACTGGTGAGAATGGGACCATTGATGGCCAAGGTGATGTCTGGTGGAATATGTGGAGGACAAGGACTCTGATGTACACTAGGCCTAATCTCGTTGAATTTATCAATTCTCATAATATCATCATTTCCAACGTAATGTTCCTGAACTCTCCGTTTTGGAACATTCATCCAGTTTACTGCAGTAACGTTGTTGTGAGATATGTCACAATATTGGCTCCTCGTGATTCCCCGAACACCGATGGAATCGATCCAGACTCGAGCAACAACGTCTGTATAGAAGATTCCTACATTTCAACTGGAGATGATCTTGTGGCTGTGAAGAGTGGATGGGACGAATATGGTATCGCTTACGGTCGTAGTAGTTACGACATTACCATCCGAAGAATATCGGGGTCAAGTCCATTTGCTGGAGTTGCTGTAGGAAGCGAAACCTCCGGTGGTGTGTCAAATGTATTAGCAGAACACCTAAGCTTATACGACATGGGAGTTGGTATCAACATCAAGACAAACATTGGAAGAGGAGGGTTCATAAAGAACATAACCATATCCGATGTCTACATGGAGAGCTCGCGAAAGGGTCTAAAGATTGCGAGCGATACTGGAGATCATCCCGACGACAAGTTCGACCCAAACGCTCTTCCAATCGTCAAAGACATCACAATTAAAAACGTTTGGGGTGAGAATGTGCAGCAAGCAGGTTCTATATATGGCTTGAGGAACTCCCCATTTACTGGTATATGTCTTTCGAACATTAACCTTCGTGGCACAGCGAGACCGAGATCAGTACCGTGGACTTGCTCGTATGTAAGTGGTGCAGCTCTCTTGGTTAGTCCATGGCCATGCACAGAGCTTACCAGCACACAGCAGGATGGTGTGTGCTCCAATAACTTCTAA

Protein sequence

MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVEFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVLAEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGAALLVSPWPCTELTSTQQDGVCSNNF
Homology
BLAST of CmoCh02G009130 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.1e-115
Identity = 208/447 (46.53%), Postives = 290/447 (64.88%), Query Frame = 0

Query: 5   FLFIFICFAALLSSPLAAGELTTCSGIVPMKY-----RSDKISIADFGGVGDGRTLNTRA 64
           F  + +  A +L   ++ GE      +  ++Y     R+   S+ DFGGVGDG+TLNT+A
Sbjct: 22  FQVVSVLVAVVLLLSVSRGECRKGRILEALEYSAISCRAHSASLVDFGGVGDGQTLNTKA 81

Query: 65  FRAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIE 124
           F+ A+  +      GG  LY+P G +LT SF+LTSH TL+L +DAV+ A QD S WPVI+
Sbjct: 82  FQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIK 141

Query: 125 PLPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNL 184
           PLPSYGRGR+   GRY S I G  + DV+ITG+NGTIDGQG +WW  +    L YTRP L
Sbjct: 142 PLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYL 201

Query: 185 VEFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVC 244
           +E + S +I ISN+  LNSP WN+HPVY  N++++ +TILAP  SPNTDGI+PDS  N  
Sbjct: 202 IELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINPDSCTNTR 261

Query: 245 IEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGV-AVGSETSGGVSN 304
           IED YI +GDD VAVKSGWDEYGIAYG  +  + IRR++  SP++ V A+GSE SGG+ +
Sbjct: 262 IEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCISPYSAVIALGSEMSGGIQD 321

Query: 305 VLAEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDP 364
           V AE +   +   GI IKT IGRGG++K+I +  + M++ +    +  + G H D+ +DP
Sbjct: 322 VRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKWAFWMTGNYGSHADNHYDP 381

Query: 365 NALPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVS 424
            A P+++ I  +++  ENV  A  + G+ + PFTGIC+SN+ +   A+ + VPWTC+ V 
Sbjct: 382 KAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVTIHLAAKAKKVPWTCTDVE 441

Query: 425 GAALLVSPWPCTELTSTQQDGVCSNNF 446
           G +  V+P PC+ L     +     NF
Sbjct: 442 GISSGVTPTPCSTLPDQGPEKTSLCNF 468

BLAST of CmoCh02G009130 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 1.8e-30
Identity = 106/375 (28.27%), Postives = 177/375 (47.20%), Query Frame = 0

Query: 41  ISIADFGGVGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPG-VYLTESFNLTSHMTLY 100
           +S++DFG  GDG+T +T+AF  A  +       G   L +P G  YL +S  LT      
Sbjct: 68  VSVSDFGAKGDGKTDDTQAFVNAWKKA--CSSNGAVNLLVPKGNTYLLKSIQLTGPCNSI 127

Query: 101 LAKD--AVIKAVQDSSNWPVIEPLPSYGRGRERPGGRYVSFIHGDGVHDVVIT-GENGTI 160
           L       + A Q  S++  I               +++ F   DGV+++ +  G+ G +
Sbjct: 128 LTVQIFGTLSASQKRSDYKDI--------------SKWIMF---DGVNNLSVDGGDTGVV 187

Query: 161 DGQGDVWW--NMWRTRTLMYTR-PNLVEFINSHNIIISNVMFLNSPFWNIHPVYCSNVVV 220
           DG G+ WW  +  R +    T+ P  + F NS ++I+ N+   N+    I    CSNV V
Sbjct: 188 DGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQV 247

Query: 221 RYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRSSYDIT 280
             V + AP DSPNTDGI   ++ N+ + +S I TGDD ++++SG           S ++ 
Sbjct: 248 SNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQNVQ 307

Query: 281 IRRISGSSPFAGVAVGS----ETSGGVSNVLAEHLSLYDMGVGINIKTNIGRGGFIKNIT 340
           I  I+   P  G+++GS     +   VS V  +   L     G+ IKT  G  G   NI 
Sbjct: 308 INDIT-CGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNII 367

Query: 341 ISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDITIKNVWGENVQQAGSIYGL-RN 400
             ++ M++ +  + I  D  D      + +A+  VK++  +++ G +  +    +   +N
Sbjct: 368 FQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQ-VKNVVYRDISGTSASENAITFNCSKN 410

Query: 401 SPFTGICLSNINLRG 404
            P  GI L  +N++G
Sbjct: 428 YPCQGIVLDRVNIKG 410

BLAST of CmoCh02G009130 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 5.3e-22
Identity = 101/380 (26.58%), Postives = 167/380 (43.95%), Query Frame = 0

Query: 41  ISIADFGGVGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPG-VYLTESF-------NL 100
           +S+++FG  GDG+T +T+AF+ A  +       G T   +P G  YL +S        +L
Sbjct: 68  VSVSNFGAKGDGKTDDTQAFKKAWKKA--CSTNGVTTFLVPKGKTYLLKSTRFRGPCKSL 127

Query: 101 TSHMTL-YLAKDAVIKAVQDSSNWPVIEPLPSYGRGRERPGGRYVSFIHGDGVHDVVITG 160
            +   L  L+        +D ++W ++E                V+ +  DG       G
Sbjct: 128 RNFQILGTLSASTKRSDYKDKNHWLILED---------------VNNLSIDG-------G 187

Query: 161 ENGTIDGQGDVWW--NMWRTRTLMYTR-PNLVEFINSHNIIISNVMFLNSPFWNIHPVYC 220
             G I+G G  WW  +    ++   T+ P  +   N  N+ + N+   N+    I    C
Sbjct: 188 STGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKC 247

Query: 221 SNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIAYGRS 280
           + V V  V I AP DSPNTDGI   ++ N+ + +S I TGDD ++++ G     I     
Sbjct: 248 NKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQI----- 307

Query: 281 SYDITIRRISGSSPFAGVAVGS----ETSGGVSNVLAEHLSLYDMGVGINIKTNIGRGGF 340
            +D+T        P  G+++GS     +   VS +  +     +   G+ IKT  G  G 
Sbjct: 308 -FDLT------CGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGT 367

Query: 341 IKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDITIKNVWGENVQQ-AGSI 400
            KNI   ++ ME+ +  + I  D  D  D   D  +   VK++  KN+ G +    A ++
Sbjct: 368 AKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITL 410

Query: 401 YGLRNSPFTGICLSNINLRG 404
                 P  GI L N+ ++G
Sbjct: 428 NCSEKYPCQGIVLENVKIKG 410

BLAST of CmoCh02G009130 vs. ExPASy Swiss-Prot
Match: P18192 (Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 GN=peh PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.0e-20
Identity = 96/304 (31.58%), Postives = 147/304 (48.36%), Query Frame = 0

Query: 84  VYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPLPSYGRGRERPGGRYVSFIHGDG 143
           V+L+   +L S ++L + K   ++AV ++ ++   E  PS     +  G    +FI    
Sbjct: 77  VFLSGPLSLPSGVSLLIDKGVTLRAVNNAKSF---ENAPSSCGVVDTNGKGCDAFITATS 136

Query: 144 VHDVVITGENGTIDGQGDV--------WWNM---WRTRTLMYTRPNLVEFINSHNIIISN 203
             +  I G  GTIDGQG V        WW++    + + L    P L++   S N  + N
Sbjct: 137 TTNSGIYGP-GTIDGQGGVKLQDKKVSWWDLAADAKVKKLKQNTPRLIQINKSKNFTLYN 196

Query: 204 VMFLNSPFWNIHPVYC--SNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDD 263
           V  +NSP  N H V+           TI  P  + NTDGIDP SS N+ I  S ISTGDD
Sbjct: 197 VSLINSP--NFHVVFSDGDGFTAWKTTIKTPSTARNTDGIDPMSSKNITIAHSNISTGDD 256

Query: 264 LVAVKSGWDEYGIAY-GRS-SYDITIRRISGSSPFAGVAVGSETSGGVSNVLAEHLSLYD 323
            VA+K        AY GRS + +I+I      +   G+++GSET  GV NV  + L +  
Sbjct: 257 NVAIK--------AYKGRSETRNISILHNEFGTGH-GMSIGSETM-GVYNVTVDDLIMTG 316

Query: 324 MGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDIT 373
              G+ IK++    G +  +  S+V M++  K + I     D   +K + + +P   DIT
Sbjct: 317 TTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVI-----DTVYEKKEGSNVPDWSDIT 359

BLAST of CmoCh02G009130 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 101.7 bits (252), Expect = 2.2e-20
Identity = 78/287 (27.18%), Postives = 128/287 (44.60%), Query Frame = 0

Query: 142 DGVHDVVITGENGTIDGQGDVWWN---MWRTRTLMYTRPNLVEFINSHNIIISNVMFLNS 201
           + V+++ + G  G IDG G +WW        +      P  V F+  +N+ +SN+   N+
Sbjct: 156 ENVNNLRVEG-GGRIDGNGHIWWPKSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENA 215

Query: 202 PFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGW 261
              ++    C NV    + + +P DSPNTDGI    + N+ I+DS + TGDD +++ SG 
Sbjct: 216 QQMHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGS 275

Query: 262 DEY---GIAYGRSSYDITIRRISGSSPFAGVAVGS----ETSGGVSNVLAEHLSLYDMGV 321
           +     GI  G               P  G+++GS     +   VSNV+    +L     
Sbjct: 276 ENVRATGITCG---------------PGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTN 335

Query: 322 GINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDITIKN 381
           G+ IKT  G  G  KNI   D+ M++    + I  D  D  +   +  +   V ++  KN
Sbjct: 336 GVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKN 395

Query: 382 VWGENVQQ-AGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYV 418
           + G + +  A      +N P  GI + N+ L    +      +CS V
Sbjct: 396 IQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKASCSNV 426

BLAST of CmoCh02G009130 vs. ExPASy TrEMBL
Match: A0A6J1H0T7 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459369 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 8.8e-262
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF
Sbjct: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPCTELTSTQQDGVCSNNF
Sbjct: 421 ALLVSPWPCTELTSTQQDGVCSNNF 445

BLAST of CmoCh02G009130 vs. ExPASy TrEMBL
Match: A0A6J1K7T4 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111490953 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 7.7e-258
Identity = 438/445 (98.43%), Postives = 440/445 (98.88%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPFFLFIFICF ALLS PLAAGELTTCSGIVPMKYRSDKISIADFGGVGDG TLNTRAF
Sbjct: 1   MLPFFLFIFICFDALLSPPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGHTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNV 
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVF 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG NVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPC+ELTSTQQDG+CSNNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSNNF 445

BLAST of CmoCh02G009130 vs. ExPASy TrEMBL
Match: A0A5A7TLS6 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001910 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.5e-241
Identity = 404/445 (90.79%), Postives = 423/445 (95.06%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           M+PF LF+FI F  L  SPLA  +L TCSGIVPMKYR+DKISIADFGG+GDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAK AVIKAVQDSSNWPVI P
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGD WWNMWRT TL YTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINS+NIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGV+NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHL+ YDMGVGINIKTNIGRGGFIKNIT+S+VYME+SRKGLKIA DTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKN WG  VQQAGSIYGLR+SPFTGICLSNIN RGT RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           AL+VSPWPC+ELTST+QDG+CS+NF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

BLAST of CmoCh02G009130 vs. ExPASy TrEMBL
Match: A0A1S3ATJ9 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.5e-241
Identity = 404/445 (90.79%), Postives = 423/445 (95.06%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           M+PF LF+FI F  L  SPLA  +L TCSGIVPMKYR+DKISIADFGG+GDGRTLNTRAF
Sbjct: 1   MVPFSLFVFIFFTTLFPSPLAVADLITCSGIVPMKYRNDKISIADFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTE+FNLTSHMTLYLAK AVIKAVQDSSNWPVI P
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTETFNLTSHMTLYLAKGAVIKAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHG GVHDVVITGENGTIDGQGD WWNMWRT TL YTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGHGVHDVVITGENGTIDGQGDAWWNMWRTGTLKYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINS+NIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGV+NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHL+ YDMGVGINIKTNIGRGGFIKNIT+S+VYME+SRKGLKIA DTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIKNITVSNVYMENSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKN WG  VQQAGSIYGLR+SPFTGICLSNIN RGT RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNAWGVKVQQAGSIYGLRDSPFTGICLSNINFRGTTRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           AL+VSPWPC+ELTST+QDG+CS+NF
Sbjct: 421 ALMVSPWPCSELTSTEQDGLCSDNF 445

BLAST of CmoCh02G009130 vs. ExPASy TrEMBL
Match: A0A6J1I1Q1 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.3e-240
Identity = 405/444 (91.22%), Postives = 422/444 (95.05%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPF LFIFI FA LL SP A  +LTTCSGIVPM YR+DKISI DFGG+GDGRTLNT+AF
Sbjct: 1   MLPFSLFIFIYFATLLPSPFAIAQLTTCSGIVPMNYRNDKISITDFGGIGDGRTLNTKAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQ+L+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQ SSNWPV+ P
Sbjct: 61  RAAIYRIQNLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQGSSNWPVVAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGD WWNMWRT TL +TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINS+NIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGV+NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVANVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHL+ YDMG+GINIKTNIGRGGFIKNIT+S+VYME SRKGLKIA DTGDHPDDKFDPNA
Sbjct: 301 AEHLNFYDMGIGINIKTNIGRGGFIKNITVSNVYMERSRKGLKIAGDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG NVQQAGSIYGLRNSPFTGICLSNI+LRG  RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNIHLRGAMRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNN 445
           ALLVSPWPC+ELTSTQQDG CS N
Sbjct: 421 ALLVSPWPCSELTSTQQDGSCSAN 444

BLAST of CmoCh02G009130 vs. NCBI nr
Match: XP_022958006.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 912.1 bits (2356), Expect = 1.8e-261
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF
Sbjct: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPCTELTSTQQDGVCSNNF
Sbjct: 421 ALLVSPWPCTELTSTQQDGVCSNNF 445

BLAST of CmoCh02G009130 vs. NCBI nr
Match: XP_023534421.1 (probable polygalacturonase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023534422.1 probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.4 bits (2336), Expect = 3.8e-259
Identity = 441/445 (99.10%), Postives = 443/445 (99.55%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPFFLFIFICF ALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF
Sbjct: 1   MLPFFLFIFICFDALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG NVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPC+ELTSTQQDG+CSNNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSNNF 445

BLAST of CmoCh02G009130 vs. NCBI nr
Match: KAG7035482.1 (putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 903.3 bits (2333), Expect = 8.4e-259
Identity = 441/445 (99.10%), Postives = 444/445 (99.78%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           M+PFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF
Sbjct: 1   MMPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKG+KIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGVKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNV GENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVRGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPC+ELTSTQQDGVCSNNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGVCSNNF 445

BLAST of CmoCh02G009130 vs. NCBI nr
Match: XP_022995393.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 899.0 bits (2322), Expect = 1.6e-257
Identity = 438/445 (98.43%), Postives = 440/445 (98.88%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           MLPFFLFIFICF ALLS PLAAGELTTCSGIVPMKYRSDKISIADFGGVGDG TLNTRAF
Sbjct: 1   MLPFFLFIFICFDALLSPPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGHTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI
Sbjct: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNV 
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVF 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA
Sbjct: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWG NVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGVNVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPC+ELTSTQQDG+CSNNF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSNNF 445

BLAST of CmoCh02G009130 vs. NCBI nr
Match: XP_038876129.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 853.2 bits (2203), Expect = 1.0e-243
Identity = 408/445 (91.69%), Postives = 426/445 (95.73%), Query Frame = 0

Query: 1   MLPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAF 60
           M+PFFLFIF  F  L  SPLA  +LTTCSGIVPMKYR+DKISI DFGG+GDGRTLNTRAF
Sbjct: 1   MVPFFLFIFFYFTTLFPSPLAVADLTTCSGIVPMKYRNDKISITDFGGIGDGRTLNTRAF 60

Query: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEP 120
           RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVI+AVQDSSNWPVI P
Sbjct: 61  RAAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDSSNWPVIAP 120

Query: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLV 180
           LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGD WWNMWRT TL +TRPNLV
Sbjct: 121 LPSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDAWWNMWRTGTLKFTRPNLV 180

Query: 181 EFINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCI 240
           EFINS+NIIISNVMFLNSPFWNIHPVYC+NVVVRYVTILAPRDSPNTDG+DPDSSNNVCI
Sbjct: 181 EFINSYNIIISNVMFLNSPFWNIHPVYCTNVVVRYVTILAPRDSPNTDGVDPDSSNNVCI 240

Query: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVL 300
           EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRR+SGSSPFAGVAVGSETSGGV NVL
Sbjct: 241 EDSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRVSGSSPFAGVAVGSETSGGVENVL 300

Query: 301 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 360
           AEHL+ YDMGVGINIKTNIGRGGFI+NIT+S+VYMESSRKGLKIA DTGDHPDDK+DPNA
Sbjct: 301 AEHLNFYDMGVGINIKTNIGRGGFIRNITVSNVYMESSRKGLKIAGDTGDHPDDKYDPNA 360

Query: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 420
           LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRG  RPRSVPWTCSYVSGA
Sbjct: 361 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGARRPRSVPWTCSYVSGA 420

Query: 421 ALLVSPWPCTELTSTQQDGVCSNNF 446
           ALLVSPWPC+ELTSTQQDG+CS+NF
Sbjct: 421 ALLVSPWPCSELTSTQQDGLCSDNF 445

BLAST of CmoCh02G009130 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 691.8 bits (1784), Expect = 3.6e-199
Identity = 326/440 (74.09%), Postives = 381/440 (86.59%), Query Frame = 0

Query: 6   LFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAFRAAIY 65
           +F+F C    +SS        TCSGIVP++YR DKISI DFGGVGDGRT+NT+AFRAAIY
Sbjct: 8   IFLFSCL--FVSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAIY 67

Query: 66  RIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPLPSYG 125
           RIQHL+RRGGTLLYIPPGVYLTESFNLTSHMTLYLAK AVI+AVQD+ NWP+I+PLPSYG
Sbjct: 68  RIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYG 127

Query: 126 RGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVEFINS 185
           RGRE PGGRY+SFIHGDG+ DVVITG+NGTIDGQG+VWWNMWR+RTL YTRPNL+EF +S
Sbjct: 128 RGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDS 187

Query: 186 HNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYI 245
             IIISNV+F NSPFWNIHPVYCSNVV+ +VTILAP+DSPNTDGIDPDSS NVCIEDSYI
Sbjct: 188 KEIIISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYI 247

Query: 246 STGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVLAEHLS 305
           STGDDLVA+KSGWD+YGIAYGR S +ITIRRI+GSSPFAG+A+GSETSGG+ N++AEH++
Sbjct: 248 STGDDLVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHIT 307

Query: 306 LYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVK 365
           L +MGVG+NIKTNIGRGG+IKNI ISDVY+++++ G+KIA DTGDHPD+ ++PNALP+VK
Sbjct: 308 LSNMGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVK 367

Query: 366 DITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGAALLVS 425
            I IKNVWG NV+ AGSI GL+ SPFTGICLS INL G+       W CS VSG +L VS
Sbjct: 368 GIHIKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKT-WKCSDVSGTSLKVS 427

Query: 426 PWPCTELTSTQQDGVCSNNF 446
           PWPC+EL +T    +CS+ F
Sbjct: 428 PWPCSELRTTGGSNLCSSTF 444

BLAST of CmoCh02G009130 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 611.7 bits (1576), Expect = 4.7e-175
Identity = 297/443 (67.04%), Postives = 357/443 (80.59%), Query Frame = 0

Query: 3   PFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAFRA 62
           P  +F+    + +L   L+ G   TCSGIVPMK+R++ +SI+DFG VGDG+TLNT+AF +
Sbjct: 7   PISVFVLFLLSTVLPHHLSLGAPITCSGIVPMKHRNEMLSISDFGAVGDGKTLNTKAFNS 66

Query: 63  AIYRIQHLRRRG-GTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPL 122
           AI RI++      GTLLY+P GVYLT+SFNLTSHMTLYLA  AVIKAVQD+  WP+ +PL
Sbjct: 67  AIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTLYLADGAVIKAVQDTEKWPLTDPL 126

Query: 123 PSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVE 182
           PSYGRGRE PG RY+SFIHGDG++DVVITG NGTIDGQG+ WWNMWR  TL +TRP L+E
Sbjct: 127 PSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDGQGEPWWNMWRHGTLKFTRPGLIE 186

Query: 183 FINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIE 242
           F NS NI++S+V+  NSPFW +HPVYCSNVVV +VTILAP DS NTDGIDPDSS+NVCIE
Sbjct: 187 FNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTILAPTDSYNTDGIDPDSSSNVCIE 246

Query: 243 DSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPFAGVAVGSETSGGVSNVLA 302
           DSYISTGDDLVAVKSGWDEYGIAY R S DITIRRI+GSSPFAG+A+GSETSGG+ NV  
Sbjct: 247 DSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRITGSSPFAGIAIGSETSGGIQNVTV 306

Query: 303 EHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNAL 362
           E+++LY+ G+GI+IKTNIGRGG I+ ITIS VY+E  R G+KI+ DTGDHPDDKF+ +AL
Sbjct: 307 ENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEKVRTGIKISGDTGDHPDDKFNTSAL 366

Query: 363 PIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGAA 422
           PIV+ ITIKNVWG  V++AG + GL++SPFT +C SN+ L GT   RS  W CS V GAA
Sbjct: 367 PIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFSNVTLTGT--KRSPIWKCSDVVGAA 426

Query: 423 LLVSPWPCTELT-STQQDGVCSN 444
             V+P PC EL+ +TQQ G C N
Sbjct: 427 DKVNPTPCPELSATTQQGGSCEN 447

BLAST of CmoCh02G009130 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 4.3e-128
Identity = 223/409 (54.52%), Postives = 289/409 (70.66%), Query Frame = 0

Query: 27  TCSGIVPMKYRSDKI--SIADFGGVGDGRTLNTRAFRAAIYRIQHLRRRGGTLLYIPPGV 86
           TCSG        ++I  SI DFGGVGDG+T NT AFR A+  ++     GG  L +P G 
Sbjct: 56  TCSGFFNHDPFPNRIVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGT 115

Query: 87  YLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPLPSYGRGRERPGGRYVSFIHGDGV 146
           +L+ SFNLTS+ TL+L + A+I   +D   WP+IEPLPSYGRGRERPGGR++S IHGD +
Sbjct: 116 WLSGSFNLTSNFTLFLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNL 175

Query: 147 HDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVEFINSHNIIISNVMFLNSPFWNIH 206
            +VVITGENGTIDGQG +WW +W  RTL++TR +L+E  NSHNI+ISN+  LNSPFW IH
Sbjct: 176 TNVVITGENGTIDGQGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIH 235

Query: 207 PVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIEDSYISTGDDLVAVKSGWDEYGIA 266
           PVYCSNVV+R +TILAP ++PNTDGIDPDSS NVCIED YI +GDDLVAVKSGWD+YG+A
Sbjct: 236 PVYCSNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMA 295

Query: 267 YGRSSYDITIRRISGSS-PFAGVAVGSETSGGVSNVLAEHLSLYDMGVGINIKTNIGRGG 326
             R S +I IRRISG++   +GV +GSE SGG+ N+  E + ++D   G+ IKT+ GRGG
Sbjct: 296 VARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGG 355

Query: 327 FIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNALPIVKDITIKNVWGENVQQAGSI 386
           +I NIT ++V +E  +  ++ +S + DH DDK+DP ALP VK I I NV   N ++A  +
Sbjct: 356 YISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPML 415

Query: 387 YGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGAALLVSPWPCTEL 433
            G+  + F  +CL N+ L G   P++  W C  VSG A  V P  C +L
Sbjct: 416 LGVEGTSFQDVCLRNVTLLGL--PKTEKWKCKDVSGYASDVFPLSCPQL 462

BLAST of CmoCh02G009130 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 5.0e-124
Identity = 215/425 (50.59%), Postives = 289/425 (68.00%), Query Frame = 0

Query: 4   FFLFIFICFAALLSSPLAAGELTTCS-GIVPMKYRSDKISIADFGGVGDGRTLNTRAFRA 63
           F L   +   A   +  + GE   C    +   +R   +SI +FG VGDG TLNT+AF+ 
Sbjct: 5   FLLLYVLLVQAFYGAWCSVGESLHCEYSNLASLHRPHSVSITEFGAVGDGVTLNTKAFQN 64

Query: 64  AIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPLP 123
           A++ +     +GG  L++P G +LT SF+L SH+TL+L K A I     S NWPV++PLP
Sbjct: 65  ALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLDKGATILGSTSSENWPVVDPLP 124

Query: 124 SYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVEF 183
           SYGRGRE PG R+ S I+G  + DVVITGENGTIDGQG VWW+ +R   L YTRP+LVE 
Sbjct: 125 SYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQGTVWWDWFRNGELNYTRPHLVEL 184

Query: 184 INSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIED 243
           +NS  +IISN+ FLNSPFWNIHPVYC +VVV+ +TILAP +SPNTDG+DPDSS NVCIED
Sbjct: 185 MNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSSTNVCIED 244

Query: 244 SYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISG-SSPFAGVAVGSETSGGVSNVLA 303
            YI TGDDLV++KSGWDEYGI+Y R S  I I R++G ++  +G+A+GSE SGGVS +  
Sbjct: 245 CYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGVSEIYI 304

Query: 304 EHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNAL 363
           + L L++   GI IKT+ GRGG+++N+ I +V +++ +K ++     G+HPD+K+DP AL
Sbjct: 305 KDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDPKAL 364

Query: 364 PIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGAA 423
           P ++ IT +NV G+ +  AG + G+    F  IC  N+ LR     +  PW CS V G +
Sbjct: 365 PAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSNVRGYS 424

Query: 424 LLVSP 427
             VSP
Sbjct: 425 QWVSP 429

BLAST of CmoCh02G009130 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 9.4e-115
Identity = 214/435 (49.20%), Postives = 287/435 (65.98%), Query Frame = 0

Query: 2   LPFFLFIFICFAALLSSPLAAGELTTCSGIVPMKYRSDKISIADFGGVGDGRTLNTRAFR 61
           +P  L   + F  LL S    G    C     +  R   +SI +FG VGDG+TLNT AF+
Sbjct: 3   MPVALVWLLAFTILLIS--GEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNTIAFQ 62

Query: 62  AAIYRIQHLRRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKDAVIKAVQDSSNWPVIEPL 121
            A++ ++    +GG  LY+PPG +LT SFNLTSH+TL+L K A I A  D S+W V+ PL
Sbjct: 63  NAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDVVSPL 122

Query: 122 PSYGRGRERPGGRYVSFIHGDGVHDVVITGENGTIDGQGDVWWNMWRTRTLMYTRPNLVE 181
           PSYGRG E PG RY S I+GD + DVVITGENGT DGQG  WW    + +L Y+RP+++E
Sbjct: 123 PSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRPHIIE 182

Query: 182 FINSHNIIISNVMFLNSPFWNIHPVYCSNVVVRYVTILAPRDSPNTDGIDPDSSNNVCIE 241
           F++S +I+ISN+ FLN+P  NIHPVYCS + +R V I    DSP+  G+ PDSS+NVCIE
Sbjct: 183 FVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDNVCIE 242

Query: 242 DSYISTGDDLVAVKSGWDEYGIAYGRSSYDITIRRISGSSPF-AGVAVGSETSGGVSNVL 301
           DS I+ G D V++KSGWD+YGI YGR +  + IR +   SP  AG++ GSE SGGVS+V 
Sbjct: 243 DSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGVSDVT 302

Query: 302 AEHLSLYDMGVGINIKTNIGRGGFIKNITISDVYMESSRKGLKIASDTGDHPDDKFDPNA 361
            E L+++   +G+  +T  GRGG+I+NITISDV + S    +     TG HPDDKFD +A
Sbjct: 303 VERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKFDRDA 362

Query: 362 LPIVKDITIKNVWGENVQQAGSIYGLRNSPFTGICLSNINLRGTARPRSVPWTCSYVSGA 421
           LP+V  I ++N  G ++  AG++ G+  SPFT ICL++I+L+  +      W CS VSG 
Sbjct: 363 LPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADIHLQTRSEE---SWICSNVSGF 422

Query: 422 ALLVSPWPCTELTST 436
           +  VSP PC EL S+
Sbjct: 423 SDDVSPEPCQELMSS 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL31.1e-11546.53Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q8RY291.8e-3028.27Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
O231475.3e-2226.58Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
P181921.0e-2031.58Endo-polygalacturonase OS=Pectobacterium carotovorum subsp. carotovorum OX=555 G... [more]
Q9SFB72.2e-2027.18Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H0T78.8e-262100.00probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459369 PE=3 SV... [more]
A0A6J1K7T47.7e-25898.43probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111490953 PE=3 SV=1[more]
A0A5A7TLS66.5e-24190.79Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3ATJ96.5e-24190.79probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482817 PE=3 SV=1[more]
A0A6J1I1Q13.3e-24091.22probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111469049 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022958006.11.8e-261100.00probable polygalacturonase [Cucurbita moschata][more]
XP_023534421.13.8e-25999.10probable polygalacturonase isoform X1 [Cucurbita pepo subsp. pepo] >XP_023534422... [more]
KAG7035482.18.4e-25999.10putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022995393.11.6e-25798.43probable polygalacturonase [Cucurbita maxima][more]
XP_038876129.11.0e-24391.69probable polygalacturonase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G23820.13.6e-19974.09Pectin lyase-like superfamily protein [more]
AT5G41870.14.7e-17567.04Pectin lyase-like superfamily protein [more]
AT4G33440.14.3e-12854.52Pectin lyase-like superfamily protein [more]
AT3G62110.15.0e-12450.59Pectin lyase-like superfamily protein [more]
AT3G16850.19.4e-11549.20Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 269..290
e-value: 3700.0
score: 3.1
coord: 325..346
e-value: 750.0
score: 8.1
coord: 364..398
e-value: 5700.0
score: 1.8
coord: 296..317
e-value: 2000.0
score: 5.1
coord: 208..234
e-value: 140.0
score: 11.3
coord: 235..267
e-value: 990.0
score: 7.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 137..418
e-value: 8.6E-42
score: 143.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 25..417
e-value: 1.7E-111
score: 375.0
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 6..442
NoneNo IPR availablePANTHERPTHR31339:SF3PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 6..442
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 34..434

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G009130.1CmoCh02G009130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0043086 negative regulation of catalytic activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004650 polygalacturonase activity
molecular_function GO:0004864 protein phosphatase inhibitor activity