CmoCh02G007840 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G007840
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionchaperone protein DnaJ isoform X1
LocationCmo_Chr02: 4858038 .. 4860577 (-)
RNA-Seq ExpressionCmoCh02G007840
SyntenyCmoCh02G007840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCGAATTGGCTACAACGCAAATCATTTCCGACGCCTTCTCAAGATCTGCTTCTCATCCTTCAATTTTCTTTTCGCGAAATCAAGCAAAATCTTCAATATCCTTCCGCCGGTTCTTCAGCTACCCGTTGTTGGCGCCGCCGAGCTTTGAATCGAGTTACTTGACGTATAGGAGGAGGAGCTGCTTAACCCTGGTGAGAGCGAGTCGCAGAGAGTCGCCGTACGAAGTGCTTGGTGTTTCTCCGACTGCAACTCCAAATGAAATCAAGAGGGCTTATCGGAAACTGGCTCTCAAGTATCATCCCGATGTCAACAAAGAGGTAACAGAGTAAATTTTGCCCTCTATTTTGAGTTACTTTTCTGCGTTTTTCTTGTTCTGGGTCGCTTTTGTGTTCTGGTAGGATTTGGATTATCTTTCTTGATAAACGAATCGAAGCTGGAAATTGCCAGAGAAAGCTTCAAGCCACTGCGATAGGAATTACTGTAAAAGAAGAACGAAGAACAAAAAAAAGTGAATTAGTTGTGGAATTTGATAGTTTCTGGGACATGTTCCAATGAACTTTCTATCATAGGAAACTAAAACCTTGTTACCCCATGAGTTTTGGAAGGGAAATTGGAGCACGAAGGGTCAAATTAGGATGTTCTAAGTTTTTTTTGGCTCTGTTTCACTTATGAAATTGGGGCTTCTTTTGAGATTGCTCTTTCAATGTTCTAGATATAAAATCTGAGTGAAATTTTCATGTTTGTTTTATGTAGCCTAATGCACAAGAGAAATTTATGCGTATAAAGCATGCATACAACACACTGCTGAATTCTGAATCTAGAAGGAAATATGATCATGGGAATCATGGATCTGACTTCTCGTTTTCGTCGAACGACTTTAGGCGAAGTAGAAACACCGAAGATGAAGAAACGTTTTACGGACTTGGTAATCTCTTTAGGCTTTTAGTGCATAATCATTCAAATTCTTGTCTGCTTTGCTAGACCTTATTGCCTTTACACAAGTGACTGAATCATGCAAAGGGTTCAGCTCACCATTTTATGATAGTATAACTAAGTTGTGCATGGTGTCTTTAAGGGAAGGTGTATAAGCTTGCAGACTGTCCTCTATTTTACTGTGTGAAACGATGTATACCAATTGTTATGTGAGATCCCACATTGGTTGGAGAGGAGAACGAAGCATTCCTTATATGGGTGTGGAAACGTCTCCCTAATAGACGCATTTAAAAACGTGAGGCTGACGACATTACGTAACGGGCCAAAGCGAACAATATATGTTAACGGTGGGCTTGGGCTGTTACATGTTATCAAGCAAAGTGTATGCCATCCGTTACTAGTCTTGATTGATACTTTTCACACCTTCTGCGAGAATTTAGATTACGTAGTCGTGCTCATCGCTCGGCTTCCTTTCCTTTATACATAATGCATATCATGTAAACTCTTGCTTGAGCAGTGTGCATCTGTTGAAATGATTTAGTGATCCTTTGAGTTCTGTTCATTTCTTCTATTTAAGATCACTTCCGTTATGCTGCAGGTAATGTTTTCAGGGATGTTCAAATAGCACTAGGTAGATTTGTTCTCTCTGACAGAGGATTAATACAGACGCTAATTATCATCTGCTTAATTTCAGAACTACCTAAACATCTTCTATTTTCTCCTTTGTATTGCCTATAAAGCAAGTCGGTCTTGGTAGCTAAAAGCTGTTTTTTTCATTACAGGTGACTTCTTTAAGGATCTTCAAGAAGAATTTCGAAACTGGGAGGCAAGTGCTTCTTCGCAAGGGACGCCGAAGAGCCTATGGGAAGAACTGTCGGTAAGATACCGATGTAGATAGAAATTTCAGTAACATATTAGAAGAATCTATTAAACCAACTGGGCAGAAAAAGAAAAGTGCTATGAATTGCAAAATGATCTCTTTGTATTTACATATTTCAGGAAATTGGGGAGGAATTTGTAGAGTTTCTAGAGAAAGAACTCAACATAACTGATGATTCAGAAGTTGAAACGAACGACAACGACAAAGCATCTCAGAAGACGGATTCTTTCAGAACTTCGAACACAGATGAAACAAGAAACAATGTTCGAAATCAAGGCAACAAGAGCAGTAGTAGTATCGAGGAGAATATCGACGAGATAGAAGCCACTCTCTTGAAGTTGAAGAAGGAATTAGGCTTATAGCTTATAGCTTATAGCATGGAAATGTAGAATGCAGTCTTTCAGCCACCTTTCTGTCAAGAACACATCAAAACAATTGGGTATCATTCTGGAAGGAGGTAACACACACTTGACAGTATATGTTCTTTACCAATTCTCTTCTGTGATGCCATTGAAATGGCATATAATTTGGCTGAGATTGGCAACATTTGGCAACTATTTTAAATTGTTCCTATTTTTTAAAAAATCAATTCTACCAGAAACAATTTACATTGCTGGAGCAAAAGCAACTCAATATTATTTGTTTCATCAAAAATCATTTGTAATATATATCAGTACAAGTAAAAGTGATTACCGGAAGCTTTTGTTGTCGCATCCGGCTGTT

mRNA sequence

ATGCTCGAATTGGCTACAACGCAAATCATTTCCGACGCCTTCTCAAGATCTGCTTCTCATCCTTCAATTTTCTTTTCGCGAAATCAAGCAAAATCTTCAATATCCTTCCGCCGGTTCTTCAGCTACCCGTTGTTGGCGCCGCCGAGCTTTGAATCGAGTTACTTGACGTATAGGAGGAGGAGCTGCTTAACCCTGGTGAGAGCGAGTCGCAGAGAGTCGCCGTACGAAGTGCTTGGTGTTTCTCCGACTGCAACTCCAAATGAAATCAAGAGGGCTTATCGGAAACTGGCTCTCAAGTATCATCCCGATGTCAACAAAGAGGTAACAGAGATTTGGATTATCTTTCTTGATAAACGAATCGAAGCTGGAAATTGCCAGAGAAAGCTTCAAGCCACTGCGATAGGAATTACTCCTAATGCACAAGAGAAATTTATGCGTATAAAGCATGCATACAACACACTGCTGAATTCTGAATCTAGAAGGAAATATGATCATGGGAATCATGGATCTGACTTCTCGTTTTCGTCGAACGACTTTAGGCGAAGTAGAAACACCGAAGATGAAGAAACGTTTTACGGACTTGATCACTTCCGTTATGCTGCAGGTAATGTTTTCAGGGATGTTCAAATAGCACTAGGTGACTTCTTTAAGGATCTTCAAGAAGAATTTCGAAACTGGGAGGCAAGTGCTTCTTCGCAAGGGACGCCGAAGAGCCTATGGGAAGAACTGTCGGAAATTGGGGAGGAATTTGTAGAGTTTCTAGAGAAAGAACTCAACATAACTGATGATTCAGAAGTTGAAACGAACGACAACGACAAAGCATCTCAGAAGACGGATTCTTTCAGAACTTCGAACACAGATGAAACAAGAAACAATGTTCGAAATCAAGGCAACAAGAGCAGTAGTAGTATCGAGGAGAATATCGACGAGATAGAAGCCACTCTCTTGAAGTTGAAGAAGGAATTAGGCTTATAGCTTATAGCTTATAGCATGGAAATGTAGAATGCAGTCTTTCAGCCACCTTTCTGTCAAGAACACATCAAAACAATTGGGTATCATTCTGGAAGGAGGTAACACACACTTGACAGTATATGTTCTTTACCAATTCTCTTCTGTGATGCCATTGAAATGGCATATAATTTGGCTGAGATTGGCAACATTTGGCAACTATTTTAAATTGTTCCTATTTTTTAAAAAATCAATTCTACCAGAAACAATTTACATTGCTGGAGCAAAAGCAACTCAATATTATTTGTTTCATCAAAAATCATTTGTAATATATATCAGTACAAGTAAAAGTGATTACCGGAAGCTTTTGTTGTCGCATCCGGCTGTT

Coding sequence (CDS)

ATGCTCGAATTGGCTACAACGCAAATCATTTCCGACGCCTTCTCAAGATCTGCTTCTCATCCTTCAATTTTCTTTTCGCGAAATCAAGCAAAATCTTCAATATCCTTCCGCCGGTTCTTCAGCTACCCGTTGTTGGCGCCGCCGAGCTTTGAATCGAGTTACTTGACGTATAGGAGGAGGAGCTGCTTAACCCTGGTGAGAGCGAGTCGCAGAGAGTCGCCGTACGAAGTGCTTGGTGTTTCTCCGACTGCAACTCCAAATGAAATCAAGAGGGCTTATCGGAAACTGGCTCTCAAGTATCATCCCGATGTCAACAAAGAGGTAACAGAGATTTGGATTATCTTTCTTGATAAACGAATCGAAGCTGGAAATTGCCAGAGAAAGCTTCAAGCCACTGCGATAGGAATTACTCCTAATGCACAAGAGAAATTTATGCGTATAAAGCATGCATACAACACACTGCTGAATTCTGAATCTAGAAGGAAATATGATCATGGGAATCATGGATCTGACTTCTCGTTTTCGTCGAACGACTTTAGGCGAAGTAGAAACACCGAAGATGAAGAAACGTTTTACGGACTTGATCACTTCCGTTATGCTGCAGGTAATGTTTTCAGGGATGTTCAAATAGCACTAGGTGACTTCTTTAAGGATCTTCAAGAAGAATTTCGAAACTGGGAGGCAAGTGCTTCTTCGCAAGGGACGCCGAAGAGCCTATGGGAAGAACTGTCGGAAATTGGGGAGGAATTTGTAGAGTTTCTAGAGAAAGAACTCAACATAACTGATGATTCAGAAGTTGAAACGAACGACAACGACAAAGCATCTCAGAAGACGGATTCTTTCAGAACTTCGAACACAGATGAAACAAGAAACAATGTTCGAAATCAAGGCAACAAGAGCAGTAGTAGTATCGAGGAGAATATCGACGAGATAGAAGCCACTCTCTTGAAGTTGAAGAAGGAATTAGGCTTATAG

Protein sequence

MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRRSCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLWEELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKSSSSIEENIDEIEATLLKLKKELGL
Homology
BLAST of CmoCh02G007840 vs. ExPASy Swiss-Prot
Match: Q3AF07 (Chaperone protein DnaJ OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.2e-10
Identity = 65/189 (34.39%), Postives = 79/189 (41.80%), Query Frame = 0

Query: 75  YEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAI 134
           YE+LGV+  ATP EIK+AYRKLA KYHPDVNK+                           
Sbjct: 6   YEILGVARNATPEEIKKAYRKLARKYHPDVNKD--------------------------- 65

Query: 135 GITPNAQEKFMRIKHAYNTLLNSESRRKYDH-GNHGSDFSFSSNDFRRSRNTEDEETFYG 194
              PNA EKF  I  AY  L + E R +YD  G+ G D +F+                 G
Sbjct: 66  --DPNAAEKFKEINEAYEVLSDPEKRARYDQFGHAGVDGNFA-----------------G 125

Query: 195 LDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLWEELSEIG-EEFVE 254
              F   AG  F D+    G  F DL +        A     P    E + E+  EE V 
Sbjct: 126 QGGFGGGAGINFEDIFSGFGG-FGDLFDMVFGSGRKARQGPVPGDDIEAVLELTLEEAVF 147

Query: 255 FLEKELNIT 262
             EKEL +T
Sbjct: 186 GGEKELRVT 147

BLAST of CmoCh02G007840 vs. ExPASy Swiss-Prot
Match: Q5UP23 (DnaJ-like protein R260 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R260 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 7.6e-10
Identity = 38/90 (42.22%), Postives = 49/90 (54.44%), Query Frame = 0

Query: 75  YEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAI 134
           YE+LG++P+A+  +IK+AYRKLA+KYHPD NK                GN          
Sbjct: 9   YEILGLTPSASEEDIKKAYRKLAIKYHPDKNK----------------GN---------- 68

Query: 135 GITPNAQEKFMRIKHAYNTLLNSESRRKYD 165
              P A+E F +I HA + L NSE RR YD
Sbjct: 69  ---PEAEEMFKKINHANSILSNSEKRRVYD 69

BLAST of CmoCh02G007840 vs. ExPASy Swiss-Prot
Match: C5D4U0 (Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.9e-09
Identity = 42/109 (38.53%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 75  YEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAI 134
           YE+LGVS  AT  EIK+AYRKL+ KYHPD+NKE                           
Sbjct: 7   YEILGVSKNATKEEIKKAYRKLSKKYHPDINKE--------------------------- 66

Query: 135 GITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSD----FSFSSNDF 180
              P+A EKF  IK AY  L + + R  YD   H         F S+DF
Sbjct: 67  ---PDAAEKFKEIKEAYEVLSDDQKRAHYDQFGHADPNQGFGGFRSDDF 85

BLAST of CmoCh02G007840 vs. ExPASy Swiss-Prot
Match: Q9KWS6 (Chaperone protein DnaJ OS=Parageobacillus thermoglucosidasius OX=1426 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.9e-09
Identity = 42/109 (38.53%), Postives = 50/109 (45.87%), Query Frame = 0

Query: 75  YEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAI 134
           YE+LGVS  AT  EIK+AYRKL+ KYHPD+NKE                           
Sbjct: 7   YEILGVSKNATKEEIKKAYRKLSKKYHPDINKE--------------------------- 66

Query: 135 GITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSD----FSFSSNDF 180
              P+A EKF  IK AY  L + + R  YD   H         F S+DF
Sbjct: 67  ---PDAAEKFKEIKEAYEVLSDDQKRAHYDQFGHADPNQGFGGFRSDDF 85

BLAST of CmoCh02G007840 vs. ExPASy Swiss-Prot
Match: Q5KWZ8 (Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 4.9e-09
Identity = 51/140 (36.43%), Postives = 61/140 (43.57%), Query Frame = 0

Query: 75  YEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAGNCQRKLQATAI 134
           YE+LGVS  AT +EIK+AYRKL+ +YHPDVNK                            
Sbjct: 7   YEILGVSKNATKDEIKKAYRKLSKQYHPDVNK---------------------------- 66

Query: 135 GITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNH--------GSDF---SFSSNDFRRSR 194
              P+A EKF  IK AY  L + E R +YD   H        G  F    F    F    
Sbjct: 67  --APDAAEKFKEIKEAYEVLSDDEKRARYDRFGHADPNETFGGGGFQGGGFDFGGFSGFG 116

Query: 195 NTED-EETFYGLDHFRYAAG 203
             ED  ETF+G    R A+G
Sbjct: 127 GFEDIFETFFGAGPRRRASG 116

BLAST of CmoCh02G007840 vs. ExPASy TrEMBL
Match: A0A6J1G620 (chaperone protein DnaJ isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451197 PE=4 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.9e-145
Identity = 287/324 (88.58%), Postives = 287/324 (88.58%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. ExPASy TrEMBL
Match: A0A6J1KXC9 (uncharacterized protein LOC111499634 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499634 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-141
Identity = 279/324 (86.11%), Postives = 284/324 (87.65%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLL PPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLVPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           +CLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  NCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFS+SSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSYSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GN FRDVQI LGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNFFRDVQITLGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EEL+EIGEEFVEFLEKELNITDDSEVE+NDN+KASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELAEIGEEFVEFLEKELNITDDSEVESNDNNKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. ExPASy TrEMBL
Match: A0A6J1G675 (chaperone protein dnaJ 20, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451197 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 276/324 (85.19%), Postives = 276/324 (85.19%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYG                  LGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYG------------------LGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 276

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 276

BLAST of CmoCh02G007840 vs. ExPASy TrEMBL
Match: A0A6J1L3S5 (uncharacterized protein LOC111499634 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499634 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 6.0e-135
Identity = 270/324 (83.33%), Postives = 275/324 (84.88%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLL PPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLVPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           +CLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  NCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFS+SSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSYSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYG                  LGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYG------------------LGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EEL+EIGEEFVEFLEKELNITDDSEVE+NDN+KASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELAEIGEEFVEFLEKELNITDDSEVESNDNNKASQKTDSFRTSNTDETRNNVRNQGNKS 276

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 276

BLAST of CmoCh02G007840 vs. ExPASy TrEMBL
Match: A0A5D3CU18 (DnaJ-like protein subfamily B member 9 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00220 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 2.2e-113
Identity = 240/328 (73.17%), Postives = 259/328 (78.96%), Query Frame = 0

Query: 1   MLELATTQ----IISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLT 60
           MLELAT Q    IISDA SRS S+PSIFF RNQ KSSI+ RR FSYPLLAP SFESSYLT
Sbjct: 1   MLELATPQNFPYIISDAISRSVSYPSIFFCRNQLKSSITNRRLFSYPLLAPQSFESSYLT 60

Query: 61  YRRRSCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFL 120
           YRR+S +TLV+ASRRESPYEVLG+SPTATPNEIKRAYRKLALKYHPDVNKE         
Sbjct: 61  YRRKSFVTLVKASRRESPYEVLGISPTATPNEIKRAYRKLALKYHPDVNKE--------- 120

Query: 121 DKRIEAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSS 180
                                PNAQEKFMRIKHAYNTLLNSESRRKYD+GNHGSDFS+SS
Sbjct: 121 ---------------------PNAQEKFMRIKHAYNTLLNSESRRKYDYGNHGSDFSYSS 180

Query: 181 NDFRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTP 240
               ++RNT+DEETFYG        GN   DVQIALGDFFKDLQEEFRNWEAS SSQGTP
Sbjct: 181 AQRSQNRNTQDEETFYGF-------GNFLEDVQIALGDFFKDLQEEFRNWEASVSSQGTP 240

Query: 241 KSLWEELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQ 300
           KSLWEEL EIGEEFVEFLEKELNITDDSE E++ N+KAS KTD+FRTSN D TRN+ R+Q
Sbjct: 241 KSLWEELGEIGEEFVEFLEKELNITDDSEAESS-NNKASDKTDTFRTSNADNTRNDGRSQ 289

Query: 301 GNKSSSSIEENIDEIEATLLKLKKELGL 325
            +K  SSIE+NIDEIEATLLKLKKELGL
Sbjct: 301 TSK-GSSIEDNIDEIEATLLKLKKELGL 289

BLAST of CmoCh02G007840 vs. NCBI nr
Match: XP_022947292.1 (chaperone protein DnaJ isoform X1 [Cucurbita moschata])

HSP 1 Score: 524.6 bits (1350), Expect = 5.9e-145
Identity = 287/324 (88.58%), Postives = 287/324 (88.58%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. NCBI nr
Match: KAG7035390.1 (Chaperone protein DnaJ, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.5 bits (1334), Expect = 4.3e-143
Identity = 283/324 (87.35%), Postives = 286/324 (88.27%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLEL TTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR
Sbjct: 1   MLELGTTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFS+SSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSYSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVE+NDN+KASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVESNDNNKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. NCBI nr
Match: XP_023007012.1 (uncharacterized protein LOC111499634 isoform X1 [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.3e-141
Identity = 279/324 (86.11%), Postives = 284/324 (87.65%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLL PPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLVPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           +CLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  NCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFS+SSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSYSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GN FRDVQI LGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNFFRDVQITLGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EEL+EIGEEFVEFLEKELNITDDSEVE+NDN+KASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELAEIGEEFVEFLEKELNITDDSEVESNDNNKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. NCBI nr
Match: XP_023532272.1 (chaperone protein DnaJ isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.5 bits (1316), Expect = 5.2e-141
Identity = 280/324 (86.42%), Postives = 283/324 (87.35%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPL AP SFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLSAPQSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVR SRRESPY+VLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRTSRRESPYDVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFS+SSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSYSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYGL       GN FRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYGL-------GNFFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVETNDN+KASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNNKASQKTDSFRTSNTDETRNNVRNQGNKS 287

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 287

BLAST of CmoCh02G007840 vs. NCBI nr
Match: XP_022947293.1 (chaperone protein dnaJ 20, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 498.8 bits (1283), Expect = 3.5e-137
Identity = 276/324 (85.19%), Postives = 276/324 (85.19%), Query Frame = 0

Query: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60
           MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR
Sbjct: 1   MLELATTQIISDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYRRR 60

Query: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRI 120
           SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE             
Sbjct: 61  SCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKE------------- 120

Query: 121 EAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180
                            PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR
Sbjct: 121 -----------------PNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSNDFR 180

Query: 181 RSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPKSLW 240
           RSRNTEDEETFYG                  LGDFFKDLQEEFRNWEASASSQGTPKSLW
Sbjct: 181 RSRNTEDEETFYG------------------LGDFFKDLQEEFRNWEASASSQGTPKSLW 240

Query: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 300
           EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS
Sbjct: 241 EELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQGNKS 276

Query: 301 SSSIEENIDEIEATLLKLKKELGL 325
           SSSIEENIDEIEATLLKLKKELGL
Sbjct: 301 SSSIEENIDEIEATLLKLKKELGL 276

BLAST of CmoCh02G007840 vs. TAIR 10
Match: AT5G59610.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.6e-55
Identity = 136/267 (50.94%), Postives = 168/267 (62.92%), Query Frame = 0

Query: 58  RRRSCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLD 117
           RRR+ + + RA  R SPYE+LGVSP+ATP +IKRAYRKLALKYHPDVNKE          
Sbjct: 60  RRRNRIFVTRA--RSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEA--------- 119

Query: 118 KRIEAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSN 177
                                NAQEKF++IKHAY TL+NS+SRRKY  G+       S+ 
Sbjct: 120 ---------------------NAQEKFLKIKHAYTTLINSDSRRKY--GSDSRATGSSTG 179

Query: 178 DFRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPK 237
              R  N++ EE FYG                  LGDFFKDLQEE++NWEASASSQG PK
Sbjct: 180 QTSRKGNSQVEEDFYG------------------LGDFFKDLQEEYKNWEASASSQGKPK 239

Query: 238 SLWEELSEIGEEFVEFLEKELNITDDSEVETNDNDKASQKTDSFRTSNTDETRNNVRNQG 297
           SLWEELSEIGEEFVEFLEKELNI+D+      DN+ +S+  + F        +++ +N  
Sbjct: 240 SLWEELSEIGEEFVEFLEKELNISDE------DNEGSSKNGERFDFEEGSTEKSSGKN-N 267

Query: 298 NKSSSSIEENIDEIEATLLKLKKELGL 325
           + + +SIE+NIDEIEATL +LKK+LGL
Sbjct: 300 SSTKNSIEDNIDEIEATLAQLKKDLGL 267

BLAST of CmoCh02G007840 vs. TAIR 10
Match: AT5G59610.2 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 8.6e-25
Identity = 80/203 (39.41%), Postives = 104/203 (51.23%), Query Frame = 0

Query: 58  RRRSCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLD 117
           RRR+ + + RA  R SPYE+LGVSP+ATP +IKRAYRKLALKYHPDVNKE          
Sbjct: 60  RRRNRIFVTRA--RSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEA--------- 119

Query: 118 KRIEAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYDHGNHGSDFSFSSN 177
                                NAQEKF++IKHAY TL+NS+SRRKY  G+       S+ 
Sbjct: 120 ---------------------NAQEKFLKIKHAYTTLINSDSRRKY--GSDSRATGSSTG 179

Query: 178 DFRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIALGDFFKDLQEEFRNWEASASSQGTPK 237
              R  N++ EE FYGL       G   RDVQI  G   K  ++  +  +     +   K
Sbjct: 180 QTSRKGNSQVEEDFYGL-------GEFVRDVQIT-GISSKIFKKSIKTGKLVRPHKENRK 220

Query: 238 SLWEELSEIGEEFVEFLEKELNI 261
              +   ++ +    FL K L +
Sbjct: 240 VFGKNYRKLEKNLWSFLRKNLTL 220

BLAST of CmoCh02G007840 vs. TAIR 10
Match: AT1G28210.1 (DNAJ heat shock family protein )

HSP 1 Score: 62.4 bits (150), Expect = 7.8e-10
Identity = 44/154 (28.57%), Postives = 72/154 (46.75%), Query Frame = 0

Query: 64  TLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAG 123
           T +  S   + Y+VLGVSP AT  EIK+++ +LA K+HPD N+                 
Sbjct: 39  TGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRN---------------- 98

Query: 124 NCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYD-----HGNHGSDFSFSSND 183
                         P+A+ KF  I+ AY TL NSE R +YD     + ++ ++    S  
Sbjct: 99  -------------NPSAKRKFQEIREAYETLGNSERREEYDKLQYRNSDYVNNDGGDSER 158

Query: 184 FRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIAL 213
           FRR+  +   +TF+ +    +    +  D+++ L
Sbjct: 159 FRRAYQSNFSDTFHKIFSEIFENNQIKPDIRVEL 163

BLAST of CmoCh02G007840 vs. TAIR 10
Match: AT1G28210.2 (DNAJ heat shock family protein )

HSP 1 Score: 62.4 bits (150), Expect = 7.8e-10
Identity = 44/154 (28.57%), Postives = 72/154 (46.75%), Query Frame = 0

Query: 64  TLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDKRIEAG 123
           T +  S   + Y+VLGVSP AT  EIK+++ +LA K+HPD N+                 
Sbjct: 39  TGINNSSARNYYDVLGVSPKATREEIKKSFHELAKKFHPDTNRN---------------- 98

Query: 124 NCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYD-----HGNHGSDFSFSSND 183
                         P+A+ KF  I+ AY TL NSE R +YD     + ++ ++    S  
Sbjct: 99  -------------NPSAKRKFQEIREAYETLGNSERREEYDKLQYRNSDYVNNDGGDSER 158

Query: 184 FRRSRNTEDEETFYGLDHFRYAAGNVFRDVQIAL 213
           FRR+  +   +TF+ +    +    +  D+++ L
Sbjct: 159 FRRAYQSNFSDTFHKIFSEIFENNQIKPDIRVEL 163

BLAST of CmoCh02G007840 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 55.5 bits (132), Expect = 9.5e-08
Identity = 62/197 (31.47%), Postives = 79/197 (40.10%), Query Frame = 0

Query: 1   MLELATTQII--SDAFSRSASHPSIFFSRNQAKSSISFRRFFSYPLLAPPSFESSYLTYR 60
           M  LA+  +I  S  F+ +A  P    S   A S       +    L+  S  SS   YR
Sbjct: 1   MAALASPSLIPSSLCFAAAADGPRSLSSNFSAFSDGGSNFRYHKSFLSLSSSSSSSTPYR 60

Query: 61  RRSCLTLVRASRRESPYEVLGVSPTATPNEIKRAYRKLALKYHPDVNKEVTEIWIIFLDK 120
            R   +LV  +     Y  LGVS +A   EIK AYR+LA +YHPDVNKE           
Sbjct: 61  NRRGRSLVVFATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDVNKE----------- 120

Query: 121 RIEAGNCQRKLQATAIGITPNAQEKFMRIKHAYNTLLNSESRRKYD-HGNHGSDFSFSSN 180
                              P A EKF  I  AY  L + + R  YD +G  G   +    
Sbjct: 121 -------------------PGATEKFKEISAAYEVLSDEQKRALYDQYGEAGVKSTVGGA 167

Query: 181 DFRRSRNTED-EETFYG 194
               + N  D  ETF+G
Sbjct: 181 SGPYTSNPFDLFETFFG 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3AF071.2e-1034.39Chaperone protein DnaJ OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161... [more]
Q5UP237.6e-1042.22DnaJ-like protein R260 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_R26... [more]
C5D4U02.9e-0938.53Chaperone protein DnaJ OS=Geobacillus sp. (strain WCH70) OX=471223 GN=dnaJ PE=3 ... [more]
Q9KWS62.9e-0938.53Chaperone protein DnaJ OS=Parageobacillus thermoglucosidasius OX=1426 GN=dnaJ PE... [more]
Q5KWZ84.9e-0936.43Chaperone protein DnaJ OS=Geobacillus kaustophilus (strain HTA426) OX=235909 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1G6202.9e-14588.58chaperone protein DnaJ isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451197 ... [more]
A0A6J1KXC91.1e-14186.11uncharacterized protein LOC111499634 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1G6751.7e-13785.19chaperone protein dnaJ 20, chloroplastic isoform X2 OS=Cucurbita moschata OX=366... [more]
A0A6J1L3S56.0e-13583.33uncharacterized protein LOC111499634 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5D3CU182.2e-11373.17DnaJ-like protein subfamily B member 9 isoform X1 OS=Cucumis melo var. makuwa OX... [more]
Match NameE-valueIdentityDescription
XP_022947292.15.9e-14588.58chaperone protein DnaJ isoform X1 [Cucurbita moschata][more]
KAG7035390.14.3e-14387.35Chaperone protein DnaJ, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023007012.12.3e-14186.11uncharacterized protein LOC111499634 isoform X1 [Cucurbita maxima][more]
XP_023532272.15.2e-14186.42chaperone protein DnaJ isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022947293.13.5e-13785.19chaperone protein dnaJ 20, chloroplastic isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G59610.13.6e-5550.94Chaperone DnaJ-domain superfamily protein [more]
AT5G59610.28.6e-2539.41Chaperone DnaJ-domain superfamily protein [more]
AT1G28210.17.8e-1028.57DNAJ heat shock family protein [more]
AT1G28210.27.8e-1028.57DNAJ heat shock family protein [more]
AT1G80030.19.5e-0831.47Molecular chaperone Hsp40/DnaJ family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 301..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..277
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 262..306
NoneNo IPR availablePANTHERPTHR43096DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATEDcoord: 59..322
NoneNo IPR availablePANTHERPTHR43096:SF47CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEINcoord: 59..322
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 75..93
score: 56.11
coord: 139..159
score: 33.95
coord: 93..108
score: 61.81
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 72..159
e-value: 3.9E-19
score: 79.6
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 74..107
e-value: 1.3E-13
score: 50.9
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 73..167
score: 18.562042
IPR001623DnaJ domainCDDcd06257DnaJcoord: 74..156
e-value: 5.69052E-18
score: 74.5041
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 63..179
e-value: 6.8E-25
score: 89.1
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 70..184
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 144..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G007840.1CmoCh02G007840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051082 unfolded protein binding