CmoCh02G007200 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G007200
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGDSL esterase/lipase
LocationCmo_Chr02: 4536454 .. 4538024 (+)
RNA-Seq ExpressionCmoCh02G007200
SyntenyCmoCh02G007200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACAAGTGAATTATCTTATTGCTGCTCTATTGCTCCAGATAGTTTGGTCACTATGTATGAGCAAATCCTGCAGTGCTTCTGATCTTAAAAATTTTGGATATTTTCCTGCTATTCTTATTTTCGGTGATTCTACGGTAGATACGGGAAATAACAACTTCATTCCCACCATTACAAAGGCTAATTACTCTCCTTATGACAAAGATTTTCACGGTCATATTGCTACTGGAAGGTTCAGTAATGAAAAACTCATTCCTGACCTGGTGGCCTCTAGGTTGGGCATAAAGGAGCTTGTTCCTCCATTTTTTTATCCAAAATTGTCAAATGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCCGTCTCAGGGTTTGATGATCTAACAACTATGATATCCGAAGTTATTCCTATGATGAAGCATGTTGATATGTCCAAGAACTATATTCAAAGACTTACTAGGATTGTAGGTGTAGATGAAAGTAAGAAGATTATTGGTAGTGCTTTAGTTATTCTTAGTGTAGGAACTAATCACTTTACCAAAAATTTCTATGACCTTCGGGTAAGGAAACAACAATACAATATTAGCACTTACCAAGATTTCATCCAAGATAGACTACAAAACTTCATCAAGGTAAATTAAACCATATGCACGCTATTAACCCTAATCAATATTTTATCGCATAATAATCCAAATTCATTCACATAAGCATATGAACTCTAACATACTAAACAGGAAATTTACGAGCTTGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCTATTGGTTGTCTTCCCATTCAAGAGACTATATCGTTTCAACCTCCTCTAAAACGAATATGTTTGGAAGATCAAAACTCGGACTCCATATCCTATAATCAGAAGCTTTCAAAGTTGTTGGGCAATCTACAATCGCAACTACATGGAAGCACAATACTGTACGCTGACATTTACACCCCTCTCATCGACATGGTCAATAATCCTCACAAGTATGGTACGTCGGTGTCCATCAAAATAGAAACCTTTATCAACTTATATGATCTAACGTCGGAAATATTTTAGAGAAATCAAGTTCTAAGAATCATTTTCAACTAAGTTTTGCTTGGGGCTTTTGGTCGCACAAGTTTTGAGCATGCAAACACAAGTTGTGGAACTGAATTGGCAGAAGAAGCGCCTCTATGTAATGCATTAACACCAATATGAAGGATACCTATTTTGGGATAACCTCCATCCACCAAAGCAGCTTACAAATTCATGACTGAATTATTTCTCAAGCAATTTCTTTAATCACCTAAATCAAAATTTAATTATTTATGGGAAATCAAAGTTTGAGAACCATTTTCAACTAATTTTTTGTTTTGGGAATTTGTTGAACAGGTTTTGAGCATACAGAGAAGAGTTGTTGTGGAATTGGATTAGTGGAAGCGGAGCCTCTATGTAATGTAATTATCCCAACGTGTAAAGATCCATCAAAATTTATGTCCTGGGATAACATTCATCCGACTGAAGCAGCCTACAAATTCGTTACTAAATCGCTTCTCAAGCAATTTTTGAATCATCTAAATTGA

mRNA sequence

ATGGCACAAGTGAATTATCTTATTGCTGCTCTATTGCTCCAGATAGTTTGGTCACTATGTATGAGCAAATCCTGCAGTGCTTCTGATCTTAAAAATTTTGGATATTTTCCTGCTATTCTTATTTTCGGTGATTCTACGGTAGATACGGGAAATAACAACTTCATTCCCACCATTACAAAGGCTAATTACTCTCCTTATGACAAAGATTTTCACGGTCATATTGCTACTGGAAGGTTCAGTAATGAAAAACTCATTCCTGACCTGGTGGCCTCTAGGTTGGGCATAAAGGAGCTTGTTCCTCCATTTTTTTATCCAAAATTGTCAAATGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCCGTCTCAGGGTTTGATGATCTAACAACTATGATATCCGAAGTTATTCCTATGATGAAGCATGTTGATATGTCCAAGAACTATATTCAAAGACTTACTAGGATTGTAGGTGTAGATGAAAGTAAGAAGATTATTGGTAGTGCTTTAGTTATTCTTAGTGTAGGAACTAATCACTTTACCAAAAATTTCTATGACCTTCGGGTAAGGAAACAACAATACAATATTAGCACTTACCAAGATTTCATCCAAGATAGACTACAAAACTTCATCAAGGAAATTTACGAGCTTGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCTATTGGTTGTCTTCCCATTCAAGAGACTATATCGTTTCAACCTCCTCTAAAACGAATATGTTTGGAAGATCAAAACTCGGACTCCATATCCTATAATCAGAAGCTTTCAAAGTTGTTGGGCAATCTACAATCGCAACTACATGGAAGCACAATACTGTACGCTGACATTTACACCCCTCTCATCGACATGGTCAATAATCCTCACAAGTATGGTTTTGAGCATACAGAGAAGAGTTGTTGTGGAATTGGATTAGTGGAAGCGGAGCCTCTATGTAATGTAATTATCCCAACGTGTAAAGATCCATCAAAATTTATGTCCTGGGATAACATTCATCCGACTGAAGCAGCCTACAAATTCGTTACTAAATCGCTTCTCAAGCAATTTTTGAATCATCTAAATTGA

Coding sequence (CDS)

ATGGCACAAGTGAATTATCTTATTGCTGCTCTATTGCTCCAGATAGTTTGGTCACTATGTATGAGCAAATCCTGCAGTGCTTCTGATCTTAAAAATTTTGGATATTTTCCTGCTATTCTTATTTTCGGTGATTCTACGGTAGATACGGGAAATAACAACTTCATTCCCACCATTACAAAGGCTAATTACTCTCCTTATGACAAAGATTTTCACGGTCATATTGCTACTGGAAGGTTCAGTAATGAAAAACTCATTCCTGACCTGGTGGCCTCTAGGTTGGGCATAAAGGAGCTTGTTCCTCCATTTTTTTATCCAAAATTGTCAAATGATGACGTGAAAACAGGAGTTAGTTTTGCATCAGCCGTCTCAGGGTTTGATGATCTAACAACTATGATATCCGAAGTTATTCCTATGATGAAGCATGTTGATATGTCCAAGAACTATATTCAAAGACTTACTAGGATTGTAGGTGTAGATGAAAGTAAGAAGATTATTGGTAGTGCTTTAGTTATTCTTAGTGTAGGAACTAATCACTTTACCAAAAATTTCTATGACCTTCGGGTAAGGAAACAACAATACAATATTAGCACTTACCAAGATTTCATCCAAGATAGACTACAAAACTTCATCAAGGAAATTTACGAGCTTGGATGTCGCAATATAGTGGTTGCTGGCCTCCCTCCTATTGGTTGTCTTCCCATTCAAGAGACTATATCGTTTCAACCTCCTCTAAAACGAATATGTTTGGAAGATCAAAACTCGGACTCCATATCCTATAATCAGAAGCTTTCAAAGTTGTTGGGCAATCTACAATCGCAACTACATGGAAGCACAATACTGTACGCTGACATTTACACCCCTCTCATCGACATGGTCAATAATCCTCACAAGTATGGTTTTGAGCATACAGAGAAGAGTTGTTGTGGAATTGGATTAGTGGAAGCGGAGCCTCTATGTAATGTAATTATCCCAACGTGTAAAGATCCATCAAAATTTATGTCCTGGGATAACATTCATCCGACTGAAGCAGCCTACAAATTCGTTACTAAATCGCTTCTCAAGCAATTTTTGAATCATCTAAATTGA

Protein sequence

MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITKANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFLNHLN
Homology
BLAST of CmoCh02G007200 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 357.5 bits (916), Expect = 1.8e-97
Identity = 169/359 (47.08%), Postives = 240/359 (66.85%), Query Frame = 0

Query: 7   LIAALLLQIVWSLCMSKSCSASDLK----NFGYFPAILIFGDSTVDTGNNNFIPTITKAN 66
           LI  ++  I+ ++  S +C A  +     N   FPAIL+FGDST+DTGNNN+I T  +AN
Sbjct: 2   LIHVIIFMIITTMQFSTTCHAYVINVTNVNVSMFPAILVFGDSTIDTGNNNYIKTYIRAN 61

Query: 67  YSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAV 126
           + PY  +F GH ATGRFSN KLIPD +AS +GIK+ VPPF  P LS+ D+ TGV FASA 
Sbjct: 62  FPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKDTVPPFLDPHLSDSDIITGVCFASAG 121

Query: 127 SGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKN 186
           SG+D+LT   +  + + K  DM ++Y++RL++IVG +++  I+  ALVI+S GTN F  N
Sbjct: 122 SGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVGDEKAASIVSEALVIVSSGTNDFNLN 181

Query: 187 FYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQP 246
            YD   R+Q+  +  YQ FI   + NF++E+Y++GCR I+V GLPP+GCLPIQ T++ Q 
Sbjct: 182 LYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIGCRKIMVLGLPPVGCLPIQMTMAMQK 241

Query: 247 PLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEH 306
             +R C++ QNSDS  +NQKL   L  +QS L GS I Y DIY  L DM  NP +YG + 
Sbjct: 242 QNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGSVIFYGDIYGALFDMATNPQRYGLKE 301

Query: 307 TEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFLNHLN 362
           T + CCG G +E   LCN +   C +P++++ WD+IHP++ AY  ++ SL++Q  + L+
Sbjct: 302 TTRGCCGTGEIELAYLCNALTRICPNPNQYLFWDDIHPSQIAYIVISLSLVEQIFHVLS 360

BLAST of CmoCh02G007200 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.7e-95
Identity = 170/315 (53.97%), Postives = 223/315 (70.79%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNF-IPTITKANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLG 95
           FPAILIFGDSTVDTGNNN+   TI KA + PY  D  GH A GR+SN K+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 96  IKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTR 155
           IKELVPPF  P +S+ D+ TGVSFASA +G+DD +++ S+ IP+ +   M KNYI RL  
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 156 IVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTYQDFIQDRLQNFIKEI 215
           IVG  ++ +II +ALV++S G N F  NFYD+  R+ +Y  I  YQ+FI  RL  F++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 216 YELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQ 275
           Y LGCRNIVV GLPP+GCLPIQ T   +  L R C+E +N DS+ YNQKL K L  +Q+ 
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 276 LHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFM 335
           L GS  LYA++Y PL+DM+ NP KYGF+ T+K CCG G +E   +CN +  TC + S  +
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 336 SWDNIHPTEAAYKFV 349
            WD+IHP+EAAY ++
Sbjct: 332 FWDSIHPSEAAYNYI 345

BLAST of CmoCh02G007200 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.5e-94
Identity = 170/327 (51.99%), Postives = 225/327 (68.81%), Query Frame = 0

Query: 24  SCSASDLKNFGYFPAILIFGDSTVDTGNNNFI-PTITKANYSPYDKDFHGHIATGRFSNE 83
           SC+A        FPAILIFGDST DTGNNN+    + KAN+ PY  D  GH A GRFSN 
Sbjct: 19  SCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNG 78

Query: 84  KLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHV 143
           KLI D+++++L IKE VPPF  P +S+ D+ TGV FASA +G+DD T++ S+ IP+ +  
Sbjct: 79  KLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQP 138

Query: 144 DMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTYQDF 203
            M KNYI RL  IVG  ++ +II +ALV++S G N F  NFYD+ +R+ +Y  I  YQDF
Sbjct: 139 SMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDF 198

Query: 204 IQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQ 263
           +  RL  F++E+Y LGCRNI+V GLPP+GCLPIQ T   +  L  IC+E +N DSI YNQ
Sbjct: 199 VLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTIL-GICVEQENKDSILYNQ 258

Query: 264 KLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNV 323
           KL K L  +Q+ L GS  LYA++Y P++DM+ NP KYGF+ T+K CCG G +E   LC  
Sbjct: 259 KLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTS 318

Query: 324 IIPTCKDPSKFMSWDNIHPTEAAYKFV 349
           +  TC + S  + WD+IHP+EAAYK++
Sbjct: 319 LSKTCPNHSDHLFWDSIHPSEAAYKYL 344

BLAST of CmoCh02G007200 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 342.0 bits (876), Expect = 8.0e-93
Identity = 168/346 (48.55%), Postives = 237/346 (68.50%), Query Frame = 0

Query: 10  ALLLQIVWSLCMSKSC-SASDLKNFGYFPAILIFGDSTVDTGNNNF-IPTITKANYSPYD 69
           A+ L +  +  +  SC +A++      FPAILIFGDSTVDTGNNN+ +PTI +A + PY 
Sbjct: 6   AITLTLFITTTLLASCDAAANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYG 65

Query: 70  KDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDD 129
            D     A GRFSN KLI D++A++L IKE +PPF  P LS+ D+ TGV FASA +G+DD
Sbjct: 66  MDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDD 125

Query: 130 LTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLR 189
           LT++ ++ I + +  +M K+YI RL  IVG  ++ +II +A V++S G N F  N+YD+ 
Sbjct: 126 LTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYDIP 185

Query: 190 VRKQQYN-ISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKR 249
            R+ +Y  IS YQDFI  RL+NF++E+Y LG RN++V GLPP+GCLPI  T  F+  + R
Sbjct: 186 SRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFR-NIFR 245

Query: 250 ICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKS 309
            CLE  N DS+ YN+KL KLL  +++ L GS  LYAD+Y P+++M+ NP KYGF+ T++ 
Sbjct: 246 FCLEHHNKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRG 305

Query: 310 CCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSL 353
           CCG G +E   +CNV  P C++ S+FM +D+IHP+EA Y  +   L
Sbjct: 306 CCGTGFLETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGNRL 350

BLAST of CmoCh02G007200 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 3.0e-92
Identity = 162/330 (49.09%), Postives = 232/330 (70.30%), Query Frame = 0

Query: 21  MSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNF-IPTITKANYSPYDKDFHGHIATGRF 80
           +S   +A++  +   FPAILIFGDSTVDTGNNN+   TI KA + PY  D   H A+GRF
Sbjct: 17  LSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPNHKASGRF 76

Query: 81  SNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMM 140
           +N K+  D++A++L IK+ VPPF  P LS+ ++ TGV FASA +G+DD T++ ++ I ++
Sbjct: 77  TNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLSTQAIRVL 136

Query: 141 KHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTY 200
               M KNYI RL  IVG  ++ +II +ALV++S G N F  N+YD+  R+ ++ +IS Y
Sbjct: 137 DQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLEFPHISGY 196

Query: 201 QDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSIS 260
           QDF+  RL NF++E+Y LGCR I+V GLPP+GCLPIQ T  F+  L R CLE +N DS+ 
Sbjct: 197 QDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFCLEQENRDSVL 256

Query: 261 YNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPL 320
           YNQKL  LL  +++ L GS ILY+++Y P++DM+ NP KYGF+ T++ CCG G +E   +
Sbjct: 257 YNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCCGTGHLETSFM 316

Query: 321 CNVIIPTCKDPSKFMSWDNIHPTEAAYKFV 349
           CN   PTC++ S+F+ +D+IHP+EA Y ++
Sbjct: 317 CNAFSPTCRNHSEFLFFDSIHPSEATYNYM 345

BLAST of CmoCh02G007200 vs. ExPASy TrEMBL
Match: A0A6J1G7I3 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451569 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.1e-177
Identity = 310/357 (86.83%), Postives = 325/357 (91.04%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL L+IVWSLCMSK CSASDLKNFG FPAILIFGDSTVDTGNNNFIPTITK
Sbjct: 1   MARVNYLIAALSLKIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTITK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF GHIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFSGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMIS+VIPMMK VDM KNYIQRLT  VGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMISKVIPMMKQVDMFKNYIQRLTESVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQ+RLQNFIKEIY+LGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQERLQNFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPLKRICLEDQNSDSISYNQKLSKLL NLQSQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLRNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. ExPASy TrEMBL
Match: A0A6J1G800 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451573 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.6e-173
Identity = 302/357 (84.59%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNY+I AL LQIVWSLCMSK CSASDLKNFG FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYIITALSLQIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD++ASRLGIKELVPPF  PKLSNDDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFPDHIATGRFSNGKLIPDMMASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVD+SKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDDSKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQD LQ+FIKEIY+LGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDTLQSFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPL RICLEDQNSDSISYNQKLSKLLGNLQ QLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLTRICLEDQNSDSISYNQKLSKLLGNLQLQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+DPSK++ WD++HPTEAAYKF+T+  L+QFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDPSKYLFWDSVHPTEAAYKFMTELFLRQFL 357

BLAST of CmoCh02G007200 vs. ExPASy TrEMBL
Match: A0A6J1L373 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499480 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 2.0e-171
Identity = 305/357 (85.43%), Postives = 317/357 (88.80%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL LQIVWSL M K CSASDLKNF  FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FIKEIYELGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           Q PL RICLEDQNSDSISYNQKLSKLLGNL SQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. ExPASy TrEMBL
Match: A0A6J1KWV1 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499475 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.7e-170
Identity = 304/357 (85.15%), Postives = 316/357 (88.52%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL LQIVWSL M K CSASDLKNF  FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FIKEIYELGCRNIVVAGLPPIGCLPIQETIS 
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISS 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           Q PL RICLEDQNSDSISYNQKLSKLLGNL SQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. ExPASy TrEMBL
Match: A0A6J1KYS8 (GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499474 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.9e-170
Identity = 303/357 (84.87%), Postives = 316/357 (88.52%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL LQIVWSL M K CSASDLKNF  FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FI+EIYELGCRNIVVAGLPPIGCLPIQETIS 
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIEEIYELGCRNIVVAGLPPIGCLPIQETISS 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           Q PL RICLEDQNSDSISYNQKLSKLLGNL SQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. NCBI nr
Match: XP_022947811.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 630.6 bits (1625), Expect = 8.6e-177
Identity = 310/357 (86.83%), Postives = 325/357 (91.04%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL L+IVWSLCMSK CSASDLKNFG FPAILIFGDSTVDTGNNNFIPTITK
Sbjct: 1   MARVNYLIAALSLKIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTITK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF GHIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFSGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMIS+VIPMMK VDM KNYIQRLT  VGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMISKVIPMMKQVDMFKNYIQRLTESVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQ+RLQNFIKEIY+LGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQERLQNFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPLKRICLEDQNSDSISYNQKLSKLL NLQSQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLRNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. NCBI nr
Match: XP_023521410.1 (GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo] >XP_023521412.1 GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.8 bits (1592), Expect = 5.7e-173
Identity = 305/357 (85.43%), Postives = 319/357 (89.36%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MAQVN+LIAAL L IVWSLC SK CSASDLK FG FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MAQVNFLIAALSLHIVWSLCTSKLCSASDLKKFGSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF GHIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVK+GVSFAS
Sbjct: 61  ANYSPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKSGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTM  +VIPMM  VDM KNYIQRLT IVG DESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMTYKVIPMMMQVDMFKNYIQRLTEIVGGDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FI+EIYELGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIEEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. NCBI nr
Match: XP_022947814.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 617.5 bits (1591), Expect = 7.5e-173
Identity = 302/357 (84.59%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNY+I AL LQIVWSLCMSK CSASDLKNFG FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYIITALSLQIVWSLCMSKLCSASDLKNFGSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD++ASRLGIKELVPPF  PKLSNDDVKTGV+FAS
Sbjct: 61  ANYSPYGKDFPDHIATGRFSNGKLIPDMMASRLGIKELVPPFLDPKLSNDDVKTGVNFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVD+SKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDDSKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQD LQ+FIKEIY+LGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDTLQSFIKEIYDLGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPL RICLEDQNSDSISYNQKLSKLLGNLQ QLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLTRICLEDQNSDSISYNQKLSKLLGNLQLQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+DPSK++ WD++HPTEAAYKF+T+  L+QFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDPSKYLFWDSVHPTEAAYKFMTELFLRQFL 357

BLAST of CmoCh02G007200 vs. NCBI nr
Match: XP_023533076.1 (GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.7e-172
Identity = 304/357 (85.15%), Postives = 316/357 (88.52%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MAQVNYLI  L L IVWSLC SK CSASDLK FG FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MAQVNYLIVVLSLHIVWSLCTSKLCSASDLKKFGSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF GHIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVK+GVSFAS
Sbjct: 61  ANYSPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKSGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTM  +VIPMM  VDM KNYIQRLT IVG DESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMTYKVIPMMMQVDMFKNYIQRLTEIVGGDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FIKEIYELGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILY DIYTPLIDMVNNPHKYGF
Sbjct: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYTDIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. NCBI nr
Match: XP_023006809.1 (GDSL esterase/lipase At2g30310-like [Cucurbita maxima])

HSP 1 Score: 611.7 bits (1576), Expect = 4.1e-171
Identity = 305/357 (85.43%), Postives = 317/357 (88.80%), Query Frame = 0

Query: 1   MAQVNYLIAALLLQIVWSLCMSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNFIPTITK 60
           MA+VNYLIAAL LQIVWSL M K CSASDLKNF  FPAILIFGDSTVDTGNNNFIPTI K
Sbjct: 1   MARVNYLIAALSLQIVWSLYMIKLCSASDLKNFRSFPAILIFGDSTVDTGNNNFIPTIIK 60

Query: 61  ANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFAS 120
           ANYSPY KDF  HIATGRFSN KLIPD+VASRLGIKELVPPF  PKLSNDDVKTGVSFAS
Sbjct: 61  ANYSPYGKDFPNHIATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFAS 120

Query: 121 AVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFT 180
           A SGFDDLTTMI +VIPMM  VDM KNYIQRLT IVGVDESKKIIGSALVILS GTN FT
Sbjct: 121 AGSGFDDLTTMIYKVIPMMMQVDMFKNYIQRLTEIVGVDESKKIIGSALVILSAGTNDFT 180

Query: 181 KNFYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240
           KNFYDL VRK QYNIS YQDFIQDRLQ+FIKEIYELGCRNIVVAGLPPIGCLPIQETISF
Sbjct: 181 KNFYDLPVRKLQYNISGYQDFIQDRLQSFIKEIYELGCRNIVVAGLPPIGCLPIQETISF 240

Query: 241 QPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGF 300
           Q PL RICLEDQNSDSISYNQKLSKLLGNL SQLHGSTILYADIYTPLIDMVNNPHKYGF
Sbjct: 241 QLPLTRICLEDQNSDSISYNQKLSKLLGNLPSQLHGSTILYADIYTPLIDMVNNPHKYGF 300

Query: 301 EHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFL 358
           EHT   CCG GL E  PLCN + PTC+D SK++ WD++HPTEAAYKF+T+  LKQFL
Sbjct: 301 EHTNTGCCGTGLAEVAPLCNALTPTCEDSSKYLFWDSVHPTEAAYKFMTELFLKQFL 357

BLAST of CmoCh02G007200 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.3e-98
Identity = 169/359 (47.08%), Postives = 240/359 (66.85%), Query Frame = 0

Query: 7   LIAALLLQIVWSLCMSKSCSASDLK----NFGYFPAILIFGDSTVDTGNNNFIPTITKAN 66
           LI  ++  I+ ++  S +C A  +     N   FPAIL+FGDST+DTGNNN+I T  +AN
Sbjct: 2   LIHVIIFMIITTMQFSTTCHAYVINVTNVNVSMFPAILVFGDSTIDTGNNNYIKTYIRAN 61

Query: 67  YSPYDKDFHGHIATGRFSNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAV 126
           + PY  +F GH ATGRFSN KLIPD +AS +GIK+ VPPF  P LS+ D+ TGV FASA 
Sbjct: 62  FPPYGCNFPGHNATGRFSNGKLIPDFIASLMGIKDTVPPFLDPHLSDSDIITGVCFASAG 121

Query: 127 SGFDDLTTMISEVIPMMKHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKN 186
           SG+D+LT   +  + + K  DM ++Y++RL++IVG +++  I+  ALVI+S GTN F  N
Sbjct: 122 SGYDNLTDRATSTLSVDKQADMLRSYVERLSQIVGDEKAASIVSEALVIVSSGTNDFNLN 181

Query: 187 FYDLRVRKQQYNISTYQDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQP 246
            YD   R+Q+  +  YQ FI   + NF++E+Y++GCR I+V GLPP+GCLPIQ T++ Q 
Sbjct: 182 LYDTPSRRQKLGVDGYQSFILSNVHNFVQELYDIGCRKIMVLGLPPVGCLPIQMTMAMQK 241

Query: 247 PLKRICLEDQNSDSISYNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEH 306
             +R C++ QNSDS  +NQKL   L  +QS L GS I Y DIY  L DM  NP +YG + 
Sbjct: 242 QNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLTGSVIFYGDIYGALFDMATNPQRYGLKE 301

Query: 307 TEKSCCGIGLVEAEPLCNVIIPTCKDPSKFMSWDNIHPTEAAYKFVTKSLLKQFLNHLN 362
           T + CCG G +E   LCN +   C +P++++ WD+IHP++ AY  ++ SL++Q  + L+
Sbjct: 302 TTRGCCGTGEIELAYLCNALTRICPNPNQYLFWDDIHPSQIAYIVISLSLVEQIFHVLS 360

BLAST of CmoCh02G007200 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.2e-96
Identity = 170/315 (53.97%), Postives = 223/315 (70.79%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNF-IPTITKANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLG 95
           FPAILIFGDSTVDTGNNN+   TI KA + PY  D  GH A GR+SN K+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 96  IKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTR 155
           IKELVPPF  P +S+ D+ TGVSFASA +G+DD +++ S+ IP+ +   M KNYI RL  
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 156 IVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTYQDFIQDRLQNFIKEI 215
           IVG  ++ +II +ALV++S G N F  NFYD+  R+ +Y  I  YQ+FI  RL  F++E+
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 216 YELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQ 275
           Y LGCRNIVV GLPP+GCLPIQ T   +  L R C+E +N DS+ YNQKL K L  +Q+ 
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 276 LHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFM 335
           L GS  LYA++Y PL+DM+ NP KYGF+ T+K CCG G +E   +CN +  TC + S  +
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 336 SWDNIHPTEAAYKFV 349
            WD+IHP+EAAY ++
Sbjct: 332 FWDSIHPSEAAYNYI 345

BLAST of CmoCh02G007200 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.0e-95
Identity = 170/327 (51.99%), Postives = 225/327 (68.81%), Query Frame = 0

Query: 24  SCSASDLKNFGYFPAILIFGDSTVDTGNNNFI-PTITKANYSPYDKDFHGHIATGRFSNE 83
           SC+A        FPAILIFGDST DTGNNN+    + KAN+ PY  D  GH A GRFSN 
Sbjct: 19  SCNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNG 78

Query: 84  KLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHV 143
           KLI D+++++L IKE VPPF  P +S+ D+ TGV FASA +G+DD T++ S+ IP+ +  
Sbjct: 79  KLISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQP 138

Query: 144 DMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTYQDF 203
            M KNYI RL  IVG  ++ +II +ALV++S G N F  NFYD+ +R+ +Y  I  YQDF
Sbjct: 139 SMFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDF 198

Query: 204 IQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQ 263
           +  RL  F++E+Y LGCRNI+V GLPP+GCLPIQ T   +  L  IC+E +N DSI YNQ
Sbjct: 199 VLKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTIL-GICVEQENKDSILYNQ 258

Query: 264 KLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNV 323
           KL K L  +Q+ L GS  LYA++Y P++DM+ NP KYGF+ T+K CCG G +E   LC  
Sbjct: 259 KLVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTS 318

Query: 324 IIPTCKDPSKFMSWDNIHPTEAAYKFV 349
           +  TC + S  + WD+IHP+EAAYK++
Sbjct: 319 LSKTCPNHSDHLFWDSIHPSEAAYKYL 344

BLAST of CmoCh02G007200 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 340.1 bits (871), Expect = 2.2e-93
Identity = 162/330 (49.09%), Postives = 232/330 (70.30%), Query Frame = 0

Query: 21  MSKSCSASDLKNFGYFPAILIFGDSTVDTGNNNF-IPTITKANYSPYDKDFHGHIATGRF 80
           +S   +A++  +   FPAILIFGDSTVDTGNNN+   TI KA + PY  D   H A+GRF
Sbjct: 17  LSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGIDLPNHKASGRF 76

Query: 81  SNEKLIPDLVASRLGIKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMM 140
           +N K+  D++A++L IK+ VPPF  P LS+ ++ TGV FASA +G+DD T++ ++ I ++
Sbjct: 77  TNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHTSLSTQAIRVL 136

Query: 141 KHVDMSKNYIQRLTRIVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQY-NISTY 200
               M KNYI RL  IVG  ++ +II +ALV++S G N F  N+YD+  R+ ++ +IS Y
Sbjct: 137 DQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSRRLEFPHISGY 196

Query: 201 QDFIQDRLQNFIKEIYELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSIS 260
           QDF+  RL NF++E+Y LGCR I+V GLPP+GCLPIQ T  F+  L R CLE +N DS+ 
Sbjct: 197 QDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFCLEQENRDSVL 256

Query: 261 YNQKLSKLLGNLQSQLHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPL 320
           YNQKL  LL  +++ L GS ILY+++Y P++DM+ NP KYGF+ T++ CCG G +E   +
Sbjct: 257 YNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCCGTGHLETSFM 316

Query: 321 CNVIIPTCKDPSKFMSWDNIHPTEAAYKFV 349
           CN   PTC++ S+F+ +D+IHP+EA Y ++
Sbjct: 317 CNAFSPTCRNHSEFLFFDSIHPSEATYNYM 345

BLAST of CmoCh02G007200 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-92
Identity = 159/315 (50.48%), Postives = 223/315 (70.79%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNF-IPTITKANYSPYDKDFHGHIATGRFSNEKLIPDLVASRLG 95
           FPAILIFGDSTVDTGNNN+ +PTI +A + PY  D     A GRFSN KLI D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 96  IKELVPPFFYPKLSNDDVKTGVSFASAVSGFDDLTTMISEVIPMMKHVDMSKNYIQRLTR 155
           IKE +PPF  P LS+ D+ TGV FASA +G+DDLT++ ++ I + +  +M K+YI RL  
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 156 IVGVDESKKIIGSALVILSVGTNHFTKNFYDLRVRKQQYN-ISTYQDFIQDRLQNFIKEI 215
           IVG  ++ +II +A V++S G N F  N+Y++  R+ +Y  IS YQDFI  RL+NF++E+
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 216 YELGCRNIVVAGLPPIGCLPIQETISFQPPLKRICLEDQNSDSISYNQKLSKLLGNLQSQ 275
           Y LG RN++V GLPP+GCLPI  T  F+  + R CLE  N DS+ YN+KL  LL  +++ 
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFR-NIFRFCLEHHNKDSVLYNEKLQNLLPQIEAS 272

Query: 276 LHGSTILYADIYTPLIDMVNNPHKYGFEHTEKSCCGIGLVEAEPLCNVIIPTCKDPSKFM 335
           L GS  LYAD+Y P+++M+ NP KYGF+ T++ CCG G +E   +CNV  P C++ S+F+
Sbjct: 273 LPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFL 332

Query: 336 SWDNIHPTEAAYKFV 349
            +D+IHP+EA Y  +
Sbjct: 333 FFDSIHPSEATYNVI 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMJ31.8e-9747.08GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2... [more]
O229271.7e-9553.97GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
O229181.5e-9451.99GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Q9SIQ28.0e-9348.55GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
Q9SJA93.0e-9249.09GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1G7I34.1e-17786.83GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145156... [more]
A0A6J1G8003.6e-17384.59GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145157... [more]
A0A6J1L3732.0e-17185.43GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499480 ... [more]
A0A6J1KWV11.7e-17085.15GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499475 ... [more]
A0A6J1KYS84.9e-17084.87GDSL esterase/lipase At2g30310-like OS=Cucurbita maxima OX=3661 GN=LOC111499474 ... [more]
Match NameE-valueIdentityDescription
XP_022947811.18.6e-17786.83GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
XP_023521410.15.7e-17385.43GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo] >XP_023521412.1... [more]
XP_022947814.17.5e-17384.59GDSL esterase/lipase At2g30310-like [Cucurbita moschata][more]
XP_023533076.11.7e-17285.15GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo][more]
XP_023006809.14.1e-17185.43GDSL esterase/lipase At2g30310-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G06990.11.3e-9847.08GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G30310.11.2e-9653.97GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G30220.11.0e-9551.99GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G24560.12.2e-9349.09GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G31540.11.8e-9250.48GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 39..350
e-value: 8.5E-33
score: 114.7
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 27..358
e-value: 7.8E-67
score: 227.9
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 35..351
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 35..351
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 146..360
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 37..353
e-value: 1.27365E-127
score: 366.941

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G007200.1CmoCh02G007200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds