CmoCh02G006360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G006360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectin lyase-like superfamily protein
LocationCmo_Chr02: 3957804 .. 3962062 (+)
RNA-Seq ExpressionCmoCh02G006360
SyntenyCmoCh02G006360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAAAAAACTTGAGTTGCGCGTACAAAATGTCCACCCCTCGGCTCCTACGCCCCTAGCCTTCTCCTTCTCCTTCTCTGTTCCATTCTCGATCATCATCGCCTCTTCTCCTCCTCTGTCCTTAATTTCTCACATGCAAATTCAAACCAAATCTCCATATTCAATTTCAATTTCATTCTGAATGGACGGTGAGAAATCGAGTTTCGGCTCTCTCATCAACCCTTCCTGCTCCTCTCTTCTCCTCCTCTTCATCTTCATCGCCATCCTCACCCTTCAAATCGCAACCAGAACTCCTTTTCCGATCTGGATGCCTTCCTCCACTCTCACCACGGACACCGATTCCGAACCGACCTGCTCCGCCTTCTTCCACAGGGATGCAGCGCCGGAGACGGTGGTTATGTCGATAACGGATTTCGGAGGAGTGGGAGACGGGAGGACTTCCAACACGGAAGCGTTCCGTAAGGCGATCGAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGGTCGTTCCCAAGGGCAGGTGGGTCACGGGCAGTTTTAATCTCACCACTAATTTCACGCTTTTTCTCCAACGTGGCGCTGTTATTTTGGGCTCACAGGTCCGCCTCTTTCTTCCCCTCTTTTTTTTTTCCCTTTCCCTCGTTAATTCAATTCAATTAAATTCCTTTTTTTCTTACTTTATATTCCAAAGATTTAAATAAATTATTAAAATTATAGGAGCCGAATTAAATAAATATATATATATATTCTGAAAACTCTGCGGTGATTTTTTTAGTGGTGGGTATTTATTTATTTTATTTTATTATGGTTTAAAAAGGACAAAAAGTTTGTGATTCTGGGGAAGAAGAAAAGGCCAAATGGAGTTTTATTACTCAGCAGAAAAGGAAAAATCTAAATGCTGACTCTGGCGGGGAGGAGACCTCCCCTTTGCTGGCGGTAGATCTGGGCCGTTACAAATGGTATTAGAGCTAGACACCGGAGAGGTAAACAGACGCTGGCCCCAAAAAGGTGGACTTGGTGGTGGTCCCACATCAATGGGAGAAAGGAACGAGTGCCAGCAACGACACTAGACCTCAATTAGAGAAAGGAATGAGTGTGAGCAAAAACACTTGGAGAAAGGAATGAGTGCCAACAAGGACACTAAGCTTCAATTGGAGAAAGGAACGAGTGTCAGCAAGAACACTGGGCCTCAATTGGAGAAAGTAACGAGTGCTAGCAAGGACACTAGGCATTGCGATGTCTCATATTTGTTGGGGAAGAAAACAAAAACACTATTATAAGGGCATGGAAACCTTCCCTAGCACTAGTGTTCAAGCCTCGAAGGAAAGTCCAAAAGGGAAAGGAAAAAAAAGACAATATCGGCTCTGAGTAATTACATACCCCAAATTAAAATAATAAATACTCTAAATTCACAAAAATAAACTTTAATTTTTATATATTTTAGATTCTTTTGGGAAAATGAAGAAAAAAAAAAGTTGTAAATTCCTTCATTGGTTGACTTTTTTTTATTTTTTATTTTGAATTATTATGAGAGGGTATTATTTGAAATTGTTGAATGAATTATTTTGTTTATATCCATCATGGTGGCATGTTAGTGTGAAAAGAAGATATGTTTTTTTAAAATATTTTATTTTTTTGTTAAAAGGATCCAGATGAGTGGCCCATTATAGAGCCACTACCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGCAGACATATCAGCCTCATTCATGGCAACGCCCTTTCCAATGTCCTCATCACAGGTACTCCCTCTTCTTCGCCTCTTTATTTATACATGTATATATTTCTATTTTGGCTCCTTCTTCAATGCTGCCATTTTATATTCCCTCAGGATTCTTGAATTTTGTAAACTAATGGCATGCTACTTTTCTTGTCTCAAGTTCATCGCTGAGCCATTTACATTGGATTTTAGGGCCCATTTTATACCATTCTTATTATTTTCCCATTTTTTTTTTGTCAAATCGGATCCTAATAATGCTTCCTCTACGTTGCCTAGTACATTGAACTGAAAATAGTGTGAACTCTTTTGGTATACTCCGTCTAATGGTCCAAGTCCCCCCCTTTTGGGGCTCAACCTCCTCGTTGGCACACCGTTTCGTGCTTGGTGTCACATTCTCACTTTGATGGCAACGGACTTGCGATTGTGCGGCACTCACTTCTCAGTAACAAGTGAGTTAAGCCTATTCGTTCTCGCGTCGTCTACAGCCAAACGACGTATGCTCGAGCCTCTGGCCTTGGTTCAAGGAAGTTTTAGAAAACTGGAGCAAAGAGATTTCGAATGAGAGTTTTGAAAGCAAGATTTGAGAAATTGAAATTGGTGTCATATGGGAATGTAAGCAAAAAGATAATAATAACGACGTACTACATATAACTATCGGGTAGGGAGAAGTTGATAGCTAGTTATATCCCCCTATAGTACATTTTTTAGACATTTCATGTCTGGCAGATCTAGACATGATTTTCCCGAAATGTCATACTTCCTAGAAATACAAAAGTAAAACACTACAACATGGAAAGACATAAAGGGTTTGGCTTGTCATGGGCAACACGCCTTGGACGAGCATAACCGTGACAACTGGCTCTGATACCATTTATAATAGCCCAAGCCCACTTCTTGTAGATATTGTCTTTCTTGAGCTTTCTCTTTCAGGCGTCTCCTCAAGGTTTTTAAAATATGTGTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGATTTGTACCCTCTACAATCGATGTCGGATCTCACAATTCGTATTGAAGTCGTTTAGCTATGTTTATGTTTGGTTTGAAGATATTCTACATTATGCAAATTTGTTGAGTGTGTGTATTTTTCCAGGAGAGAATGGAACTATTGATGGTCAGGGGAAGATGTGGTGGGACCTATGGTGGAACAGGACATTAAATCACACAAGGGGCCATCTTATAGAGCTCATAAACTCCCACAACATTCTCATTTCCAATCTCACCTTAAAAAATTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGGTTTCGATCTCGGTTTACTCTGTTCGATCCACGACTTGTATTTGGCTCTTAAACTTCTCTTCTTCTATATTTCAGCAACGTTGTTATCAAAAACATGACGATCTTGGCTCCCCTCAACGCCCCGAACACTGACGGTATCGATCCAGGTATAGCTTCTTTTCTAAATAATATACACAATTAGGATCTAGAAAACAATGGTGAAGTTCTAAATTCCAAATGCTCGTCTTACACTCTAGATTCGAGTACTAATATCTGTATCGAGGACTGTTACATCGAGAGCGGAGATGATCTAGTTGCGGTGAAAAGTGGATGGGATCAATATGGAATCAACCTTGCACGTCCGAGCTCAAATATCATTATAAGACGAGTATCAGGCACCACTCCAACCTGTTCTGGAGTCGGAATAGGAAGCGAGATGTCGGGGGGAATATCAAACATAACGATCGAGGATTTAAACATTTGGGATTCAGCAGCCGGTGTTCGAATCAAATCGGACCAAGGAAGAGGAGGGTACATTGCAAATATTAGTATAAGCAACTTTGTAATGAACAGAGTAAAGATGGCTATAAGGTTCAGCAAGGGTTCAAACGACCATCCGGATGAAGAATGGGATCCAAAGGCAGTTCCCATGGTTAAAGGTATTTTCATAAGCAATATCACGAGCTTAAATTCAACAAAGGCTCCTGTTCTGAATGGCATAGAGGGCACATTATTCGATGGTGTGTGTATGAAAAATGTCACTATTCTTGGTCTCGCTCCCTCCGCAAAGTGGCACTGTGCTTTCGTGTCGGGCTTTAGCAGTGCGGTGTTTCCTACGCCATGTCCTCAGCTGCAAAACATCACGGTCTCATCGATGTGCTCGTGATTTTAAGCTTCTGCAGGCACAGTTTTGCTTACTTTTGGGCATCACATGAGACCATGGCATATGCTTGGAGCTTTTCAGTTTTCAAAATTTGAGTTATATTACGACGATGGATCGATCGAGGACGTGCTGCTAGCTAGACATGAGCAGCAGCTACTGAGGTGTGATGTGTGTAAGTTTTTATGGTGAAAAAGTGGTTTTATTTAACAAAATTCACACATGAGATTGTGAGATTTTAGTGACTGACCCAACCATCCACATGTTGTACAAAGTGAGGGATATGTGTTTGTAACATTTCATAGTGATTATTATTACAATTTTTTTGTTTGGAAATAAGAGGAAAGTTAGGC

mRNA sequence

AAAAGAAAAAACTTGAGTTGCGCGTACAAAATGTCCACCCCTCGGCTCCTACGCCCCTAGCCTTCTCCTTCTCCTTCTCTGTTCCATTCTCGATCATCATCGCCTCTTCTCCTCCTCTGTCCTTAATTTCTCACATGCAAATTCAAACCAAATCTCCATATTCAATTTCAATTTCATTCTGAATGGACGGTGAGAAATCGAGTTTCGGCTCTCTCATCAACCCTTCCTGCTCCTCTCTTCTCCTCCTCTTCATCTTCATCGCCATCCTCACCCTTCAAATCGCAACCAGAACTCCTTTTCCGATCTGGATGCCTTCCTCCACTCTCACCACGGACACCGATTCCGAACCGACCTGCTCCGCCTTCTTCCACAGGGATGCAGCGCCGGAGACGGTGGTTATGTCGATAACGGATTTCGGAGGAGTGGGAGACGGGAGGACTTCCAACACGGAAGCGTTCCGTAAGGCGATCGAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGGTCGTTCCCAAGGGCAGGTGGGTCACGGGCAGTTTTAATCTCACCACTAATTTCACGCTTTTTCTCCAACGTGGCGCTGTTATTTTGGGCTCACAGGATCCAGATGAGTGGCCCATTATAGAGCCACTACCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGCAGACATATCAGCCTCATTCATGGCAACGCCCTTTCCAATGTCCTCATCACAGGAGAGAATGGAACTATTGATGGTCAGGGGAAGATGTGGTGGGACCTATGGTGGAACAGGACATTAAATCACACAAGGGGCCATCTTATAGAGCTCATAAACTCCCACAACATTCTCATTTCCAATCTCACCTTAAAAAATTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGCAACGTTGTTATCAAAAACATGACGATCTTGGCTCCCCTCAACGCCCCGAACACTGACGGTATCGATCCAGATTCGAGTACTAATATCTGTATCGAGGACTGTTACATCGAGAGCGGAGATGATCTAGTTGCGGTGAAAAGTGGATGGGATCAATATGGAATCAACCTTGCACGTCCGAGCTCAAATATCATTATAAGACGAGTATCAGGCACCACTCCAACCTGTTCTGGAGTCGGAATAGGAAGCGAGATGTCGGGGGGAATATCAAACATAACGATCGAGGATTTAAACATTTGGGATTCAGCAGCCGGTGTTCGAATCAAATCGGACCAAGGAAGAGGAGGGTACATTGCAAATATTAGTATAAGCAACTTTGTAATGAACAGAGTAAAGATGGCTATAAGGTTCAGCAAGGGTTCAAACGACCATCCGGATGAAGAATGGGATCCAAAGGCAGTTCCCATGGTTAAAGGTATTTTCATAAGCAATATCACGAGCTTAAATTCAACAAAGGCTCCTGTTCTGAATGGCATAGAGGGCACATTATTCGATGGTGTGTGTATGAAAAATGTCACTATTCTTGGTCTCGCTCCCTCCGCAAAGTGGCACTGTGCTTTCGTGTCGGGCTTTAGCAGTGCGGTGTTTCCTACGCCATGTCCTCAGCTGCAAAACATCACGGTCTCATCGATGTGCTCGTGATTTTAAGCTTCTGCAGGCACAGTTTTGCTTACTTTTGGGCATCACATGAGACCATGGCATATGCTTGGAGCTTTTCAGTTTTCAAAATTTGAGTTATATTACGACGATGGATCGATCGAGGACGTGCTGCTAGCTAGACATGAGCAGCAGCTACTGAGGTGTGATGTGTGTAAGTTTTTATGGTGAAAAAGTGGTTTTATTTAACAAAATTCACACATGAGATTGTGAGATTTTAGTGACTGACCCAACCATCCACATGTTGTACAAAGTGAGGGATATGTGTTTGTAACATTTCATAGTGATTATTATTACAATTTTTTTGTTTGGAAATAAGAGGAAAGTTAGGC

Coding sequence (CDS)

ATGGACGGTGAGAAATCGAGTTTCGGCTCTCTCATCAACCCTTCCTGCTCCTCTCTTCTCCTCCTCTTCATCTTCATCGCCATCCTCACCCTTCAAATCGCAACCAGAACTCCTTTTCCGATCTGGATGCCTTCCTCCACTCTCACCACGGACACCGATTCCGAACCGACCTGCTCCGCCTTCTTCCACAGGGATGCAGCGCCGGAGACGGTGGTTATGTCGATAACGGATTTCGGAGGAGTGGGAGACGGGAGGACTTCCAACACGGAAGCGTTCCGTAAGGCGATCGAGTACATGCGGCGGTTCGGCGACAAAGGCGGGTCTAAACTGGTCGTTCCCAAGGGCAGGTGGGTCACGGGCAGTTTTAATCTCACCACTAATTTCACGCTTTTTCTCCAACGTGGCGCTGTTATTTTGGGCTCACAGGATCCAGATGAGTGGCCCATTATAGAGCCACTACCTTCTTATGGAAGAGGAAGAGAGAGATTGGGAGGCAGACATATCAGCCTCATTCATGGCAACGCCCTTTCCAATGTCCTCATCACAGGAGAGAATGGAACTATTGATGGTCAGGGGAAGATGTGGTGGGACCTATGGTGGAACAGGACATTAAATCACACAAGGGGCCATCTTATAGAGCTCATAAACTCCCACAACATTCTCATTTCCAATCTCACCTTAAAAAATTCTCCCTTCTGGACCGTCCATCCCGTCTATTGCAGCAACGTTGTTATCAAAAACATGACGATCTTGGCTCCCCTCAACGCCCCGAACACTGACGGTATCGATCCAGATTCGAGTACTAATATCTGTATCGAGGACTGTTACATCGAGAGCGGAGATGATCTAGTTGCGGTGAAAAGTGGATGGGATCAATATGGAATCAACCTTGCACGTCCGAGCTCAAATATCATTATAAGACGAGTATCAGGCACCACTCCAACCTGTTCTGGAGTCGGAATAGGAAGCGAGATGTCGGGGGGAATATCAAACATAACGATCGAGGATTTAAACATTTGGGATTCAGCAGCCGGTGTTCGAATCAAATCGGACCAAGGAAGAGGAGGGTACATTGCAAATATTAGTATAAGCAACTTTGTAATGAACAGAGTAAAGATGGCTATAAGGTTCAGCAAGGGTTCAAACGACCATCCGGATGAAGAATGGGATCCAAAGGCAGTTCCCATGGTTAAAGGTATTTTCATAAGCAATATCACGAGCTTAAATTCAACAAAGGCTCCTGTTCTGAATGGCATAGAGGGCACATTATTCGATGGTGTGTGTATGAAAAATGTCACTATTCTTGGTCTCGCTCCCTCCGCAAAGTGGCACTGTGCTTTCGTGTCGGGCTTTAGCAGTGCGGTGTTTCCTACGCCATGTCCTCAGCTGCAAAACATCACGGTCTCATCGATGTGCTCGTGA

Protein sequence

MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Homology
BLAST of CmoCh02G006360 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.9e-116
Identity = 206/405 (50.86%), Postives = 280/405 (69.14%), Query Frame = 0

Query: 74  SITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTGSFNLTTNFTLFLQ 133
           S+ DFGGVGDG+T NT+AF+ A+  + ++G +GG++L VP G+W+TGSF+LT++FTLFL 
Sbjct: 64  SLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLH 123

Query: 134 RGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVLITGENGTIDGQGK 193
           R AV+L SQD  +WP+I+PLPSYGRGR+   GR+ SLI G  L++V+ITG+NGTIDGQG 
Sbjct: 124 RDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGG 183

Query: 194 MWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYCSNVVIKNMTILAP 253
           +WW  +    L +TR +LIEL+ S +I ISNLTL NSP W VHPVY  N++I+ +TILAP
Sbjct: 184 LWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAP 243

Query: 254 LNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTT 313
           + +PNTDGI+PDS TN  IEDCYI SGDD VAVKSGWD+YGI    P+  ++IRR++  +
Sbjct: 244 VRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRRLTCIS 303

Query: 314 PTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKM 373
           P  + + +GSEMSGGI ++  ED+   +S +G+RIK+  GRGGY+ +I +    M  +K 
Sbjct: 304 PYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTMKTMKW 363

Query: 374 AIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVT 433
           A   +     H D  +DPKA P+++GI   ++ + N + A  L GI    F G+C+ NVT
Sbjct: 364 AFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGICISNVT 423

Query: 434 ILGLAPSAK---WHCAFVSGFSSAVFPTPCPQL--QNITVSSMCS 474
           I  LA  AK   W C  V G SS V PTPC  L  Q    +S+C+
Sbjct: 424 I-HLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCN 467

BLAST of CmoCh02G006360 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 1.0e-26
Identity = 104/387 (26.87%), Postives = 174/387 (44.96%), Query Frame = 0

Query: 62  FHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGR-WVTG 121
           F R +      +S++DFG  GDG+T +T+AF  A  + +     G   L+VPKG  ++  
Sbjct: 57  FVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLK 116

Query: 122 SFNLT--TNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSN 181
           S  LT   N  L +Q    +  SQ   ++  I                    I  + ++N
Sbjct: 117 SIQLTGPCNSILTVQIFGTLSASQKRSDYKDIS-----------------KWIMFDGVNN 176

Query: 182 VLIT-GENGTIDGQGKMWW--DLWWNRTLNHTRG-HLIELINSHNILISNLTLKNSPFWT 241
           + +  G+ G +DG G+ WW      N+    T+    +   NS ++++ NL ++N+    
Sbjct: 177 LSVDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQ 236

Query: 242 VHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYG 301
           +    CSNV + N+ + AP ++PNTDGI   ++ NI + +  I +GDD ++++SG     
Sbjct: 237 ISIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG----- 296

Query: 302 INLARPSSNIIIRRVSGTTPTCSGVGIGS----EMSGGISNITIEDLNIWDSAAGVRIKS 361
                 S N+ I  +  T     G+ IGS         +S +T++   +  +  GVRIK+
Sbjct: 297 ------SQNVQINDI--TCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKT 356

Query: 362 DQGRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLN- 421
            QG  G  +NI   N  M+ VK  I   +   D      +  AV  VK +   +I+  + 
Sbjct: 357 YQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISGTSA 410

Query: 422 STKAPVLNGIEGTLFDGVCMKNVTILG 437
           S  A   N  +     G+ +  V I G
Sbjct: 417 SENAITFNCSKNYPCQGIVLDRVNIKG 410

BLAST of CmoCh02G006360 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.9e-26
Identity = 112/422 (26.54%), Postives = 181/422 (42.89%), Query Frame = 0

Query: 73  MSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW-------VTGSFNLT 132
           + +T FG VGDG T +++AF KA E +      G  + VVP G           GS   T
Sbjct: 24  LDVTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQPLKFQGSCKST 83

Query: 133 TNFTLFLQRGAVILGSQ-----DPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 192
             F   L  G ++  S+     D D+W +   +                          L
Sbjct: 84  PVFVQML--GKLVAPSKGNWKGDKDQWILFTDIEG------------------------L 143

Query: 193 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 252
           +   +G I+GQG  WW+         +R   ++  + +N+ +S LT  +SP   +H   C
Sbjct: 144 VIEGDGEINGQGSSWWE------HKGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISEC 203

Query: 253 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 312
           + V I ++ I AP ++PNTDGID  +S+N+ I+DC I +GDD +A+ SG           
Sbjct: 204 NYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG----------- 263

Query: 313 SSNIIIRRVSGTTPTCSGVGIGSEMSGG----ISNITIEDLNIWDSAAGVRIKSDQGRGG 372
           +SNI I  +        G+ IGS    G    + N+ +++ N   +  G RIK+ QG  G
Sbjct: 264 TSNIHISGID--CGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSG 323

Query: 373 YIANISISNFVMNRVKMAI---RFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKA 432
           Y   I+ +   ++ V+  I   +F  G +    ++    AV + K +F + I +  S   
Sbjct: 324 YARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYG 383

Query: 433 PVLNGIEGTLFDGVCMKNVTI----LGLAPSAKWHCAFVSGFSSAVFP-TPCPQLQNITV 471
                 E      + ++++ I     G    A+  C  V G S+   P   C +L     
Sbjct: 384 VDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVRGASTIAVPGLECLELSTDMF 398

BLAST of CmoCh02G006360 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.4e-25
Identity = 108/391 (27.62%), Postives = 173/391 (44.25%), Query Frame = 0

Query: 69  ETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW-------VTGS 128
           E   +S+++FG  GDG+T +T+AF+KA  + +     G +  +VPKG+          G 
Sbjct: 64  EASTVSVSNFGAKGDGKTDDTQAFKKA--WKKACSTNGVTTFLVPKGKTYLLKSTRFRGP 123

Query: 129 FNLTTNFTLFLQRGAVILGS--QDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 188
                NF +     A    S  +D + W I+E +                    N LS  
Sbjct: 124 CKSLRNFQILGTLSASTKRSDYKDKNHWLILEDV--------------------NNLS-- 183

Query: 189 LITGENGTIDGQGKMWW--DLWWNRTLNHTRG-HLIELINSHNILISNLTLKNSPFWTVH 248
           +  G  G I+G GK WW      +++   T+    + L N  N+ + NL +KN+    + 
Sbjct: 184 IDGGSTGIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQIS 243

Query: 249 PVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGIN 308
              C+ V + N+ I AP ++PNTDGI   ++ NI + +  I +GDD ++++ G       
Sbjct: 244 IEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDG------- 303

Query: 309 LARPSSNIIIRRVSGTTPTCSGVGIGS----EMSGGISNITIEDLNIWDSAAGVRIKSDQ 368
               + N+ I  +  T     G+ IGS         +S I ++     +S  GVRIK+ Q
Sbjct: 304 ----TQNLQIFDL--TCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQ 363

Query: 369 GRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTK 428
           G  G   NI   N  M  VK  I   +   D  D+  D ++   VK +   NI+  ++T 
Sbjct: 364 GGSGTAKNIKFQNIRMENVKNPIIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATD 416

Query: 429 APV-LNGIEGTLFDGVCMKNVTILGLAPSAK 443
             + LN  E     G+ ++NV I G   S K
Sbjct: 424 VAITLNCSEKYPCQGIVLENVKIKGGTASCK 416

BLAST of CmoCh02G006360 vs. ExPASy Swiss-Prot
Match: P05117 (Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 5.1e-23
Identity = 98/385 (25.45%), Postives = 162/385 (42.08%), Query Frame = 0

Query: 72  VMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGR-------WVTGSFNL 131
           V+++  FG  GDG+T +  AF +A  +      +   + VVPK +         +G    
Sbjct: 75  VINVLSFGAKGDGKTYDNIAFEQA--WNEACSSRTPVQFVVPKNKNYLLKQITFSGPCRS 134

Query: 132 TTNFTLF--LQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVLIT 191
           + +  +F  L+  + I   +D   W   + + +                        L+ 
Sbjct: 135 SISVKIFGSLEASSKISDYKDRRLWIAFDSVQN------------------------LVV 194

Query: 192 GENGTIDGQGKMWW--DLWWNRTLN-HTRGHLIELINSHNILISNLTLKNSPFWTVHPVY 251
           G  GTI+G G++WW      N++L        +   N  N+ ++NL  KN+    +    
Sbjct: 195 GGGGTINGNGQVWWPSSCKINKSLPCRDAPTALTFWNCKNLKVNNLKSKNAQQIHIKFES 254

Query: 252 CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR 311
           C+NVV  N+ I A   +PNTDG+   ++  I I D  I +GDD +++ SG          
Sbjct: 255 CTNVVASNLMINASAKSPNTDGVHVSNTQYIQISDTIIGTGDDCISIVSG---------- 314

Query: 312 PSSNIIIRRVSGTTPTCS---GVGIGSEMSGG----ISNITIEDLNIWDSAAGVRIKSDQ 371
                  + V  T  TC    G+ IGS  SG     +SN+T+ +  I  +  GVRIK+ Q
Sbjct: 315 ------SQNVQATNITCGPGHGISIGSLGSGNSEAYVSNVTVNEAKIIGAENGVRIKTWQ 374

Query: 372 GRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTK 431
           G  G  +NI   N  M  VK  I   +   D  +      +   VK +   NI   ++TK
Sbjct: 375 GGSGQASNIKFLNVEMQDVKYPIIIDQNYCDRVEPCIQQFSAVQVKNVVYENIKGTSATK 417

Query: 432 APVLNGIEGTL-FDGVCMKNVTILG 437
             +          +G+ M+N+ ++G
Sbjct: 435 VAIKFDCSTNFPCEGIIMENINLVG 417

BLAST of CmoCh02G006360 vs. ExPASy TrEMBL
Match: A0A6J1G526 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 6.4e-279
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60
           MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA
Sbjct: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60

Query: 61  FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG 120
           FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG
Sbjct: 61  FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG 120

Query: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180
           SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL
Sbjct: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180

Query: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240
           ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC
Sbjct: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240

Query: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300
           SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP
Sbjct: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300

Query: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360
           SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN
Sbjct: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360

Query: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE 420
           ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE
Sbjct: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE 420

Query: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Sbjct: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

BLAST of CmoCh02G006360 vs. ExPASy TrEMBL
Match: A0A6J1L474 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 2.6e-272
Identity = 462/474 (97.47%), Postives = 468/474 (98.73%), Query Frame = 0

Query: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTL-TTDTDSEPTCS 60
           MDGEKSSF  LINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTL TTDTDSEPTCS
Sbjct: 1   MDGEKSSFAPLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLATTDTDSEPTCS 60

Query: 61  AFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVT 120
           AFFHRDAAP+TVVM ITDFGGVGDGRTSNTEAFRKAIEYMRRFG+ GGSKL+VPKG W+T
Sbjct: 61  AFFHRDAAPDTVVMLITDFGGVGDGRTSNTEAFRKAIEYMRRFGETGGSKLIVPKGSWIT 120

Query: 121 GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180
           GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV
Sbjct: 121 GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180

Query: 181 LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY 240
           LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY
Sbjct: 181 LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY 240

Query: 241 CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR 300
           CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR
Sbjct: 241 CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR 300

Query: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIA 360
           PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITI+DLNIWDSAAGVRIKSDQGRGGYIA
Sbjct: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNIWDSAAGVRIKSDQGRGGYIA 360

Query: 361 NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGI 420
           NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVP+VKGIFISNITSLNSTKAPVLNGI
Sbjct: 361 NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLNGI 420

Query: 421 EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Sbjct: 421 EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474

BLAST of CmoCh02G006360 vs. ExPASy TrEMBL
Match: A0A0A0LJW9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 2.8e-242
Identity = 412/476 (86.55%), Postives = 439/476 (92.23%), Query Frame = 0

Query: 1   MDGEKSS--FGSLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLTTDTDSEPT 60
           MD +KS   FG  INPSCSSLLLLFIF+AILT QIATRT PFP W+P S         PT
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 60

Query: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120
           CSAFF  DA    VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 61  CSAFFRPDAT-AGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 120

Query: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT+NFTLFLQRGA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 VTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 181 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300
           VYCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWDQYGINL
Sbjct: 241 VYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINL 300

Query: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGY 360
           ARPSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGY
Sbjct: 301 ARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360

Query: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLN 420
           IAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPKAVP VKGIFI+N+ SLNSTKAPVL 
Sbjct: 361 IANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLY 420

Query: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GI GT +DGVCMKNVTILGL PSAKWHCAFVSGFS++VFPTPCPQLQN T SS+CS
Sbjct: 421 GIAGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPTPCPQLQNTTFSSLCS 470

BLAST of CmoCh02G006360 vs. ExPASy TrEMBL
Match: A0A5A7TGP3 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold703G00210 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 410/476 (86.13%), Postives = 438/476 (92.02%), Query Frame = 0

Query: 1   MDGEKSS--FGSLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLTTDTDSEPT 60
           MD +KS   FG  INPSCSSLLLLFIF+AILT QIATRT PFP W+P S         PT
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 60

Query: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120
           CSAFF  D     VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 61  CSAFFRPDVT-AAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 120

Query: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT+NFTLFLQRGA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 VTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 181 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300
           VYCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWDQYGINL
Sbjct: 241 VYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINL 300

Query: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGY 360
           ARPSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGY
Sbjct: 301 ARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360

Query: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLN 420
           IAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPKAVP VKGIFI+N+ SLNSTKAPVL+
Sbjct: 361 IANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLD 420

Query: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GI GT +DGVCMKNVTILGLAPSAKWHCAFVSGFS++VFP PCPQLQN T S +CS
Sbjct: 421 GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 470

BLAST of CmoCh02G006360 vs. ExPASy TrEMBL
Match: A0A1S3CDX7 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 1.4e-241
Identity = 410/476 (86.13%), Postives = 438/476 (92.02%), Query Frame = 0

Query: 1   MDGEKSS--FGSLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLTTDTDSEPT 60
           MD +KS   FG  INPSCSSLLLLFIF+AILT QIATRT PFP W+P S         PT
Sbjct: 42  MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 101

Query: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120
           CSAFF  D     VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 102 CSAFFRPDVT-AAVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 161

Query: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT+NFTLFLQRGA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 162 VTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 221

Query: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 222 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 281

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300
           VYCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWDQYGINL
Sbjct: 282 VYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINL 341

Query: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGY 360
           ARPSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGY
Sbjct: 342 ARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 401

Query: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLN 420
           IAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPKAVP VKGIFI+N+ SLNSTKAPVL+
Sbjct: 402 IANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLD 461

Query: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GI GT +DGVCMKNVTILGLAPSAKWHCAFVSGFS++VFP PCPQLQN T S +CS
Sbjct: 462 GIAGTSYDGVCMKNVTILGLAPSAKWHCAFVSGFSTSVFPMPCPQLQNTTFSPLCS 511

BLAST of CmoCh02G006360 vs. NCBI nr
Match: XP_022946971.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 969.1 bits (2504), Expect = 1.3e-278
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60
           MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA
Sbjct: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60

Query: 61  FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG 120
           FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG
Sbjct: 61  FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG 120

Query: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180
           SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL
Sbjct: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180

Query: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240
           ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC
Sbjct: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240

Query: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300
           SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP
Sbjct: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300

Query: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360
           SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN
Sbjct: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360

Query: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE 420
           ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE
Sbjct: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE 420

Query: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Sbjct: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

BLAST of CmoCh02G006360 vs. NCBI nr
Match: XP_023533571.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.8 bits (2485), Expect = 2.1e-276
Identity = 468/473 (98.94%), Postives = 471/473 (99.58%), Query Frame = 0

Query: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60
           MDGEKSSF SLINPSCSSLLLLFIFIA+LTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA
Sbjct: 1   MDGEKSSFASLINPSCSSLLLLFIFIAVLTLQIATRTPFPIWMPSSTLTTDTDSEPTCSA 60

Query: 61  FFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTG 120
           FFHRD APETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFG+KGGSKLVVPKGRWVTG
Sbjct: 61  FFHRDVAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGEKGGSKLVVPKGRWVTG 120

Query: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180
           SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL
Sbjct: 121 SFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVL 180

Query: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240
           ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC
Sbjct: 181 ITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYC 240

Query: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300
           SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP
Sbjct: 241 SNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARP 300

Query: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360
           SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN
Sbjct: 301 SSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIAN 360

Query: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIE 420
           ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVP+VKGIFISNITSLNSTKAPVLNGIE
Sbjct: 361 ISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLNGIE 420

Query: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Sbjct: 421 GTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 473

BLAST of CmoCh02G006360 vs. NCBI nr
Match: XP_023007164.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 947.2 bits (2447), Expect = 5.4e-272
Identity = 462/474 (97.47%), Postives = 468/474 (98.73%), Query Frame = 0

Query: 1   MDGEKSSFGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTL-TTDTDSEPTCS 60
           MDGEKSSF  LINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTL TTDTDSEPTCS
Sbjct: 1   MDGEKSSFAPLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLATTDTDSEPTCS 60

Query: 61  AFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVT 120
           AFFHRDAAP+TVVM ITDFGGVGDGRTSNTEAFRKAIEYMRRFG+ GGSKL+VPKG W+T
Sbjct: 61  AFFHRDAAPDTVVMLITDFGGVGDGRTSNTEAFRKAIEYMRRFGETGGSKLIVPKGSWIT 120

Query: 121 GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180
           GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV
Sbjct: 121 GSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNV 180

Query: 181 LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY 240
           LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY
Sbjct: 181 LITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVY 240

Query: 241 CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR 300
           CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR
Sbjct: 241 CSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLAR 300

Query: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIA 360
           PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITI+DLNIWDSAAGVRIKSDQGRGGYIA
Sbjct: 301 PSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIDDLNIWDSAAGVRIKSDQGRGGYIA 360

Query: 361 NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGI 420
           NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVP+VKGIFISNITSLNSTKAPVLNGI
Sbjct: 361 NISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPIVKGIFISNITSLNSTKAPVLNGI 420

Query: 421 EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS
Sbjct: 421 EGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474

BLAST of CmoCh02G006360 vs. NCBI nr
Match: XP_038900759.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 859.8 bits (2220), Expect = 1.1e-245
Identity = 418/478 (87.45%), Postives = 448/478 (93.72%), Query Frame = 0

Query: 1   MDGEKS--SFGSLINPSCSSLLLLFIFIAILTLQIATRTPFP-IWMPSSTLTTDTDSEPT 60
           MDGEKS  SFG  INPSCSSLLLLFIF+AILT QIATRTPFP IW+PS         +PT
Sbjct: 1   MDGEKSKFSFGPPINPSCSSLLLLFIFLAILTFQIATRTPFPTIWLPSDF------QQPT 60

Query: 61  CSAFFHRDAAPET--VVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKG 120
           CSAFF RDAA +T  VVMSITDFGGVGDG+TSNTEAFRKAIEYMRRFG+KGGSKL VPKG
Sbjct: 61  CSAFFRRDAAADTAAVVMSITDFGGVGDGKTSNTEAFRKAIEYMRRFGEKGGSKLTVPKG 120

Query: 121 RWVTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNA 180
            WVTGSFNLT+NFTLFLQRGA+IL SQDP+EWPIIEPLPSYGRGRERLGGRHISLIHGNA
Sbjct: 121 SWVTGSFNLTSNFTLFLQRGALILASQDPEEWPIIEPLPSYGRGRERLGGRHISLIHGNA 180

Query: 181 LSNVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTV 240
           LSNV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELI+SHNILISNLTLKNSPFWT+
Sbjct: 181 LSNVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELIDSHNILISNLTLKNSPFWTI 240

Query: 241 HPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGI 300
           HPVYCSNVVIK+MTILAPLNAPNTDGIDPDSS N+CIEDCYIESGDDLVAVKSGWDQYG+
Sbjct: 241 HPVYCSNVVIKDMTILAPLNAPNTDGIDPDSSINVCIEDCYIESGDDLVAVKSGWDQYGM 300

Query: 301 NLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRG 360
           NLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAG+RIKSDQGRG
Sbjct: 301 NLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGIRIKSDQGRG 360

Query: 361 GYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPV 420
           GYIAN+SI+NFVMNRVKMAIRFSKGSNDHPDE+WDPKAVP VKGIFI+N+ SLNSTKAPV
Sbjct: 361 GYIANVSITNFVMNRVKMAIRFSKGSNDHPDEQWDPKAVPKVKGIFITNLISLNSTKAPV 420

Query: 421 LNGIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           L+GI+GT +DGVCMKNVTILGLAPSAKWHCAF+SGFS++VFP PC QLQN T SS+CS
Sbjct: 421 LDGIKGTSYDGVCMKNVTILGLAPSAKWHCAFISGFSTSVFPMPCLQLQNTTFSSLCS 472

BLAST of CmoCh02G006360 vs. NCBI nr
Match: XP_004147365.1 (probable polygalacturonase [Cucumis sativus] >KGN62200.1 hypothetical protein Csa_016932 [Cucumis sativus])

HSP 1 Score: 847.4 bits (2188), Expect = 5.8e-242
Identity = 412/476 (86.55%), Postives = 439/476 (92.23%), Query Frame = 0

Query: 1   MDGEKSS--FGSLINPSCSSLLLLFIFIAILTLQIATRT-PFPIWMPSSTLTTDTDSEPT 60
           MD +KS   FG  INPSCSSLLLLFIF+AILT QIATRT PFP W+P S         PT
Sbjct: 1   MDSDKSKFYFGPPINPSCSSLLLLFIFLAILTFQIATRTPPFPFWLPPSDFQL-----PT 60

Query: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120
           CSAFF  DA    VVMSITDFGGVGDG+TSNTEAFRKAI+YMRRFGDKGGSKL VPKG W
Sbjct: 61  CSAFFRPDAT-AGVVMSITDFGGVGDGKTSNTEAFRKAIQYMRRFGDKGGSKLTVPKGSW 120

Query: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           VTGSFNLT+NFTLFLQRGA+IL SQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS
Sbjct: 121 VTGSFNLTSNFTLFLQRGALILASQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180

Query: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTLNHTRGHL+ELINSHNILISNLT KNSPFWT+HP
Sbjct: 181 NVVITGENGTIDGQGKMWWELWWNRTLNHTRGHLVELINSHNILISNLTFKNSPFWTIHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300
           VYCSNVVIK+MTILAPLNAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWDQYGINL
Sbjct: 241 VYCSNVVIKDMTILAPLNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGINL 300

Query: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGY 360
           ARPSSNI+IRRVSGTTPTCSGVGIGSEMSGGISNITIEDLN+WDSAAG+RIKSDQGRGGY
Sbjct: 301 ARPSSNIVIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNVWDSAAGIRIKSDQGRGGY 360

Query: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLN 420
           IAN+SI+NFVMNRVKMAIRFS+GSNDHPDE++DPKAVP VKGIFI+N+ SLNSTKAPVL 
Sbjct: 361 IANVSITNFVMNRVKMAIRFSRGSNDHPDEQFDPKAVPKVKGIFITNLMSLNSTKAPVLY 420

Query: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNITVSSMCS 474
           GI GT +DGVCMKNVTILGL PSAKWHCAFVSGFS++VFPTPCPQLQN T SS+CS
Sbjct: 421 GIAGTSYDGVCMKNVTILGLTPSAKWHCAFVSGFSTSVFPTPCPQLQNTTFSSLCS 470

BLAST of CmoCh02G006360 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 694.1 bits (1790), Expect = 7.7e-200
Identity = 326/466 (69.96%), Postives = 396/466 (84.98%), Query Frame = 0

Query: 1   MDGEKSS---FGSLINPSCSSLLLLFIFIAILTLQIATRTPFPIWMPSSTLTTDTDSEPT 60
           M+ EK+      ++  PS + LLL+F  +AIL+LQI++ +  P+W+P    T+  D   T
Sbjct: 1   MEPEKNPTTVLSAITRPSWAFLLLVFTVLAILSLQISSNSFLPLWIP----TSQYDDPVT 60

Query: 61  CSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRW 120
           CS FF+ D  P  +VMSITDFGGVGDG+TSNT AFR+A+ ++  F  +GG++L VP+G W
Sbjct: 61  CSGFFNHDPFPNRIVMSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTW 120

Query: 121 VTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALS 180
           ++GSFNLT+NFTLFL+RGA+ILGS+D DEWPIIEPLPSYGRGRER GGRHISLIHG+ L+
Sbjct: 121 LSGSFNLTSNFTLFLERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLT 180

Query: 181 NVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHP 240
           NV+ITGENGTIDGQGKMWW+LWWNRTL HTRGHLIEL NSHNILISNLTL NSPFWT+HP
Sbjct: 181 NVVITGENGTIDGQGKMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHP 240

Query: 241 VYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINL 300
           VYCSNVVI+NMTILAP+NAPNTDGIDPDSSTN+CIEDCYIESGDDLVAVKSGWDQYG+ +
Sbjct: 241 VYCSNVVIRNMTILAPMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAV 300

Query: 301 ARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGY 360
           ARPSSNI+IRR+SGTT TCSGVGIGSEMSGGI NIT+ED+++WDSAAG+RIK+D+GRGGY
Sbjct: 301 ARPSSNIVIRRISGTTRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGY 360

Query: 361 IANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLN 420
           I+NI+ +N ++ +VK+ IRFS GSNDH D++WDPKA+P VKGI+ISN+ SLNS KAP+L 
Sbjct: 361 ISNITFNNVLLEKVKVPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLL 420

Query: 421 GIEGTLFDGVCMKNVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQL 464
           G+EGT F  VC++NVT+LGL  + KW C  VSG++S VFP  CPQL
Sbjct: 421 GVEGTSFQDVCLRNVTLLGLPKTEKWKCKDVSGYASDVFPLSCPQL 462

BLAST of CmoCh02G006360 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 4.8e-133
Identity = 220/387 (56.85%), Postives = 291/387 (75.19%), Query Frame = 0

Query: 73  MSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTGSFNLTTNFTLFL 132
           +SIT+FG VGDG T NT+AF+ A+ Y+  F DKGG+KL VP G+W+TGSF+L ++ TL+L
Sbjct: 43  VSITEFGAVGDGVTLNTKAFQNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWL 102

Query: 133 QRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVLITGENGTIDGQG 192
            +GA ILGS   + WP+++PLPSYGRGRE  G RH SLI+G  L++V+ITGENGTIDGQG
Sbjct: 103 DKGATILGSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQG 162

Query: 193 KMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYCSNVVIKNMTILA 252
            +WWD + N  LN+TR HL+EL+NS  ++ISNLT  NSPFW +HPVYC +VV+KN+TILA
Sbjct: 163 TVWWDWFRNGELNYTRPHLVELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILA 222

Query: 253 PLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGT 312
           PL +PNTDG+DPDSSTN+CIEDCYI +GDDLV++KSGWD+YGI+ ARPSS I I R++G 
Sbjct: 223 PLESPNTDGVDPDSSTNVCIEDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQ 282

Query: 313 TPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVK 372
           T + SG+ IGSEMSGG+S I I+DL++++S  G+RIK+  GRGGY+ N+ I N  ++ VK
Sbjct: 283 TTSSSGIAIGSEMSGGVSEIYIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVK 342

Query: 373 MAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNV 432
            AIRF+    +HPDE++DPKA+P ++ I   N+       A +L GIEG +F  +C  NV
Sbjct: 343 KAIRFTGKYGEHPDEKYDPKALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNV 402

Query: 433 TILGLAPSAK--WHCAFVSGFSSAVFP 458
           T+     S K  W C+ V G+S  V P
Sbjct: 403 TLRVKKNSKKSPWECSNVRGYSQWVSP 429

BLAST of CmoCh02G006360 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 462.2 bits (1188), Expect = 4.9e-130
Identity = 225/422 (53.32%), Postives = 302/422 (71.56%), Query Frame = 0

Query: 53  DSEPTCSAFFHRDAAPETVVMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVV 112
           DSE TCS         + +  SITDFGGVGDGRT NT+AFR AI  ++    +GG+ L +
Sbjct: 23  DSEATCSGIVPLRYRYDKI--SITDFGGVGDGRTVNTKAFRAAIYRIQHLKRRGGTLLYI 82

Query: 113 PKGRWVTGSFNLTTNFTLFLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIH 172
           P G ++T SFNLT++ TL+L +GAVI   QD   WP+I+PLPSYGRGRE  GGR++S IH
Sbjct: 83  PPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSYGRGRELPGGRYMSFIH 142

Query: 173 GNALSNVLITGENGTIDGQGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPF 232
           G+ L +V+ITG+NGTIDGQG++WW++W +RTL +TR +LIE  +S  I+ISN+  +NSPF
Sbjct: 143 GDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKDSKEIIISNVIFQNSPF 202

Query: 233 WTVHPVYCSNVVIKNMTILAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQ 292
           W +HPVYCSNVVI ++TILAP ++PNTDGIDPDSS N+CIED YI +GDDLVA+KSGWDQ
Sbjct: 203 WNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSYISTGDDLVAIKSGWDQ 262

Query: 293 YGINLARPSSNIIIRRVSGTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQ 352
           YGI   RPSSNI IRR++G++P  +G+ IGSE SGGI NI  E + + +   GV IK++ 
Sbjct: 263 YGIAYGRPSSNITIRRITGSSP-FAGIAIGSETSGGIKNIIAEHITLSNMGVGVNIKTNI 322

Query: 353 GRGGYIANISISNFVMNRVKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTK 412
           GRGGYI NI IS+  ++  K  I+ +  + DHPDE ++P A+P+VKGI I N+  +N   
Sbjct: 323 GRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVVKGIHIKNVWGVNVRN 382

Query: 413 APVLNGIEGTLFDGVCMKNVTILGLAPSAK-WHCAFVSGFSSAVFPTPCPQLQNITVSSM 472
           A  + G++G+ F G+C+  + + G   S K W C+ VSG S  V P PC +L+    S++
Sbjct: 383 AGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVSPWPCSELRTTGGSNL 441

Query: 473 CS 474
           CS
Sbjct: 443 CS 441

BLAST of CmoCh02G006360 vs. TAIR 10
Match: AT5G41870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 5.8e-123
Identity = 206/397 (51.89%), Postives = 291/397 (73.30%), Query Frame = 0

Query: 72  VMSITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKG-GSKLVVPKGRWVTGSFNLTTNFTL 131
           ++SI+DFG VGDG+T NT+AF  AI+ +R   +   G+ L VP+G ++T SFNLT++ TL
Sbjct: 44  MLSISDFGAVGDGKTLNTKAFNSAIDRIRNSNNSNEGTLLYVPRGVYLTQSFNLTSHMTL 103

Query: 132 FLQRGAVILGSQDPDEWPIIEPLPSYGRGRERLGGRHISLIHGNALSNVLITGENGTIDG 191
           +L  GAVI   QD ++WP+ +PLPSYGRGRE  G R+IS IHG+ L++V+ITG NGTIDG
Sbjct: 104 YLADGAVIKAVQDTEKWPLTDPLPSYGRGREHPGRRYISFIHGDGLNDVVITGRNGTIDG 163

Query: 192 QGKMWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYCSNVVIKNMTI 251
           QG+ WW++W + TL  TR  LIE  NS NIL+S++ L+NSPFWT+HPVYCSNVV+ ++TI
Sbjct: 164 QGEPWWNMWRHGTLKFTRPGLIEFNNSTNILVSHVVLQNSPFWTLHPVYCSNVVVHHVTI 223

Query: 252 LAPLNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVS 311
           LAP ++ NTDGIDPDSS+N+CIED YI +GDDLVAVKSGWD+YGI   RPS +I IRR++
Sbjct: 224 LAPTDSYNTDGIDPDSSSNVCIEDSYISTGDDLVAVKSGWDEYGIAYNRPSRDITIRRIT 283

Query: 312 GTTPTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNR 371
           G++P  +G+ IGSE SGGI N+T+E++ +++S  G+ IK++ GRGG I  I+IS   + +
Sbjct: 284 GSSP-FAGIAIGSETSGGIQNVTVENITLYNSGIGIHIKTNIGRGGSIQGITISGVYLEK 343

Query: 372 VKMAIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMK 431
           V+  I+ S  + DHPD++++  A+P+V+GI I N+  +   +A ++ G++ + F  +C  
Sbjct: 344 VRTGIKISGDTGDHPDDKFNTSALPIVRGITIKNVWGIKVERAGMVQGLKDSPFTNLCFS 403

Query: 432 NVTILGLAPSAKWHCAFVSGFSSAVFPTPCPQLQNIT 468
           NVT+ G   S  W C+ V G +  V PTPCP+L   T
Sbjct: 404 NVTLTGTKRSPIWKCSDVVGAADKVNPTPCPELSATT 439

BLAST of CmoCh02G006360 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 422.2 bits (1084), Expect = 5.6e-118
Identity = 204/393 (51.91%), Postives = 275/393 (69.97%), Query Frame = 0

Query: 75  ITDFGGVGDGRTSNTEAFRKAIEYMRRFGDKGGSKLVVPKGRWVTGSFNLTTNFTLFLQR 134
           +TDFG VGDG+TSNT+AFR AI  + +    GG++LVVP G+W+TGSFNLT++FTLF+QR
Sbjct: 47  LTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGSFNLTSHFTLFIQR 106

Query: 135 GAVILGSQDPDEWPIIEPLPSYGRGRERLG-GRHISLIHGNALSNVLITGENGTIDGQGK 194
           GA IL SQD  EWP+I PLPSYG+GR+  G GR  SLI G  L++V+ITG NGTI+GQG+
Sbjct: 107 GATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVVITGNNGTINGQGQ 166

Query: 195 MWWDLWWNRTLNHTRGHLIELINSHNILISNLTLKNSPFWTVHPVYCSNVVIKNMTILAP 254
            WWD +  +    TR +LIE++ S NI ISN+TL +SP W +HPVYC++V++K++T+LAP
Sbjct: 167 YWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYCNSVIVKSVTVLAP 226

Query: 255 LNAPNTDGIDPDSSTNICIEDCYIESGDDLVAVKSGWDQYGINLARPSSNIIIRRVSGTT 314
           +  PNTDGI+PDS TN  IEDCYI SGDD +AVKSGWDQYGI    P+  + IRR++  +
Sbjct: 227 VTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCIS 286

Query: 315 PTCSGVGIGSEMSGGISNITIEDLNIWDSAAGVRIKSDQGRGGYIANISISNFVMNRVKM 374
           P  +GV +GSEMSGGI ++ IED+ + ++ + +RIK+  GRG Y+ +I      M  +K 
Sbjct: 287 PKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKY 346

Query: 375 AIRFSKGSNDHPDEEWDPKAVPMVKGIFISNITSLNSTKAPVLNGIEGTLFDGVCMKNVT 434
               S     HPDE +DPKA+P +  I   ++T+ N T +  L+GI+   F G+C+ NVT
Sbjct: 347 VFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVT 406

Query: 435 ILGLAPSAK---WHCAFVSGFSSAVFPTPCPQL 464
           I  LA  AK   W+C  V+G +S V P PC  L
Sbjct: 407 I-ALAAKAKKMQWNCTDVAGVTSRVTPEPCSLL 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL33.9e-11650.86Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q8RY291.0e-2626.87Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
Q9LW072.9e-2626.54Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
O231471.4e-2527.62Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
P051175.1e-2325.45Polygalacturonase-2 OS=Solanum lycopersicum OX=4081 GN=PG2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G5266.4e-279100.00probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111450990 PE=3 SV... [more]
A0A6J1L4742.6e-27297.47probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111499740 PE=3 SV=1[more]
A0A0A0LJW92.8e-24286.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G334510 PE=3 SV=1[more]
A0A5A7TGP31.4e-24186.13Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CDX71.4e-24186.13probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103499630 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022946971.11.3e-278100.00probable polygalacturonase [Cucurbita moschata][more]
XP_023533571.12.1e-27698.94probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_023007164.15.4e-27297.47probable polygalacturonase [Cucurbita maxima][more]
XP_038900759.11.1e-24587.45probable polygalacturonase [Benincasa hispida][more]
XP_004147365.15.8e-24286.55probable polygalacturonase [Cucumis sativus] >KGN62200.1 hypothetical protein Cs... [more]
Match NameE-valueIdentityDescription
AT4G33440.17.7e-20069.96Pectin lyase-like superfamily protein [more]
AT3G62110.14.8e-13356.85Pectin lyase-like superfamily protein [more]
AT4G23820.14.9e-13053.32Pectin lyase-like superfamily protein [more]
AT5G41870.15.8e-12351.89Pectin lyase-like superfamily protein [more]
AT3G48950.15.6e-11851.91Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 267..299
e-value: 410.0
score: 9.7
coord: 240..266
e-value: 680.0
score: 8.4
coord: 329..350
e-value: 24.0
score: 13.9
coord: 301..323
e-value: 4800.0
score: 2.3
coord: 397..431
e-value: 3800.0
score: 3.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 54..449
e-value: 1.9E-112
score: 378.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 170..448
e-value: 2.9E-43
score: 148.2
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 11..472
NoneNo IPR availablePANTHERPTHR31339:SF4PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 11..472
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 66..464

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G006360.1CmoCh02G006360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004650 polygalacturonase activity