CmoCh02G006080 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G006080
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionPectinesterase
LocationCmo_Chr02: 3762699 .. 3764686 (-)
RNA-Seq ExpressionCmoCh02G006080
SyntenyCmoCh02G006080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTTCAAGTGCTATCTCCCTCCTCGGAGTCTTGACTCTCCTCTACCTATTCTCCTTCTCATCCTCCGCCATCCATAGCCCAGTTCCGGCGGCGGATATCTGCCATTCCACAACCTCTCCATCCTTCTGCAAAGCCTTCCTCCCCAACTCCCTTGCCACCGTCCACTCCCATTGCCGCTTCACTCTCCGCCATGCCCTCGCCCATGCACGCTCCTTCCTTGCCCTCGTTGACAGCCACCTCGCCCTCTTCCCCTCCCTCGCTGCCCTCCACGACTGCCGCTCCCTCGCTGCGGCTAACCTCGACTTCCTATCCAACACCTTCCCCACAGTCAACACCACCACCACCACAAACGCCCTTCCCTTCTACAAAGCCGATGAGATGGTGTCGTTGCTTAGCGCAGTCATCACCAATGAAAACACTTGTTACGAAGGGCTGCTGAGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGTTGATAAGGTGTTGAAGGCCATCTCCTTCGATACAAAGCTTTATAGTTTGTACCTTTCTTTGTTCAAAATGGGTTGGGTTTCTAATGATGTCAAGGCTCCTCATGTCCCAAAGATGAAGCATTTTGGGCGTGGAAAGGGGCAGTTGAAGCTCAACATGTCCCCCAAAAACCGTGCCTATTATGAGCGCTTGATGCATAGGAGAAAGCCACCGGCTAGGAGGCTTCTTCAAACCAATTCTAATGAAGATGATGGAATTTTGGTCAATGGTAATAATTTGTAATTGAAATTAAAAAATCTTATTTTATTTGTTACGCGGTTCGAGGGCGGATACTTGGTTGGATGGGAGGATTGTTGGGATACTTTATTTATTCGTTTGTTTTATAGGTATTGTTGCGGTGGATCCGAATGGAACATATGACTTCACCACCATCAACGCCGCCATTCAGGCAGCTCCCAACAACTCCGTCGCCGCCGATGGATATTTCTTGATCTTCGTCACTGCCGGAGTTTACAACGAGAGCGTGATGGTTCCCAAACACAAAAAGTATGTGTTGATGATTGGAGAAGGCAACAACCAAACCATCATCACCGGGAGAAAAAACGTCCCCGATGGCTCTACCACTTTCAATAGCGGAACCGTCAGTGAGTTACTCTTTCCAGTGAATTATTTACTCTCCATTGGTACGAGGCCTTGAGCAAAGTCACAGAGCTTATGCTCAAAGTGGACAATATCGTACCACTGTGTTCCTAACCTTATTTAATATAAAGTTGTGATTAATCGAGGGATAAATATTTCAAATCTCTCTATTGCTCGCAGTTGTGGAGGGAACTGGGTTTTTCGGGGTGAACCTGACGATCACCAACACAGCCGGACCCAACAAATTCCAAGCCGTGGCATTGCGAGTGAGTGCCGACATGACGACGTTCTACAGTTGCATATTCGAAGGGTATCAAGACACTCTCTACCCCCACTCTCTTCGACAATTCTATAGAGAGTGCGACATCTACGGAACTATAGACTTTATTTTGGGGGACGCGGCTGTGGTGTTCCAAAACTGCAACATTTACGCCCGCCTCCCAATGAAAGGCCAATACATTGTCATAACGGCGCAAGGCCGAACTGACCCAAATCAAAACACCGGAATCTCCATCCACAACTGCACCATCAAGCCCACTGCGCAGCTCGCAGCCAGCCCCACCATCAAGAGCTACTTCGGGAGGCCGTGGAAGGAGTACGCTCGAACAGTTTACATGCAGTCGTTTATCGATGCATTTATCGACCCAGCAGGGTGGAATGTGTGGGACGGGACTTTGAATTTGAACACGTCGTATTACGCTGAGTTTAATAACTCGGGGCTGGGATCTGACACTTCGCGGCGGGCTAAATGGGCGGTCGGTTTGATCAACGCCACCGTGGCTAATAACTTCACGGTGTCTCCGTTCTTGGGTGGAGATCAATGGCTACCGCCGACCACCGTGCCGTACACTGGTGGTTTGATATCATAA

mRNA sequence

ATGGGTTCTTCAAGTGCTATCTCCCTCCTCGGAGTCTTGACTCTCCTCTACCTATTCTCCTTCTCATCCTCCGCCATCCATAGCCCAGTTCCGGCGGCGGATATCTGCCATTCCACAACCTCTCCATCCTTCTGCAAAGCCTTCCTCCCCAACTCCCTTGCCACCGTCCACTCCCATTGCCGCTTCACTCTCCGCCATGCCCTCGCCCATGCACGCTCCTTCCTTGCCCTCGTTGACAGCCACCTCGCCCTCTTCCCCTCCCTCGCTGCCCTCCACGACTGCCGCTCCCTCGCTGCGGCTAACCTCGACTTCCTATCCAACACCTTCCCCACAGTCAACACCACCACCACCACAAACGCCCTTCCCTTCTACAAAGCCGATGAGATGGTGTCGTTGCTTAGCGCAGTCATCACCAATGAAAACACTTGTTACGAAGGGCTGCTGAGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGTTGATAAGGTGTTGAAGGCCATCTCCTTCGATACAAAGCTTTATAGTTTGTACCTTTCTTTGTTCAAAATGGGTTGGGTTTCTAATGATGTCAAGGCTCCTCATGTCCCAAAGATGAAGCATTTTGGGCGTGGAAAGGGGCAGTTGAAGCTCAACATGTCCCCCAAAAACCGTGCCTATTATGAGCGCTTGATGCATAGGAGAAAGCCACCGGCTAGGAGGCTTCTTCAAACCAATTCTAATGAAGATGATGGAATTTTGGTCAATGGTATTGTTGCGGTGGATCCGAATGGAACATATGACTTCACCACCATCAACGCCGCCATTCAGGCAGCTCCCAACAACTCCGTCGCCGCCGATGGATATTTCTTGATCTTCGTCACTGCCGGAGTTTACAACGAGAGCGTGATGGTTCCCAAACACAAAAAGTATGTGTTGATGATTGGAGAAGGCAACAACCAAACCATCATCACCGGGAGAAAAAACGTCCCCGATGGCTCTACCACTTTCAATAGCGGAACCGTCATTGTGGAGGGAACTGGGTTTTTCGGGGTGAACCTGACGATCACCAACACAGCCGGACCCAACAAATTCCAAGCCGTGGCATTGCGAGTGAGTGCCGACATGACGACGTTCTACAGTTGCATATTCGAAGGGTATCAAGACACTCTCTACCCCCACTCTCTTCGACAATTCTATAGAGAGTGCGACATCTACGGAACTATAGACTTTATTTTGGGGGACGCGGCTGTGGTGTTCCAAAACTGCAACATTTACGCCCGCCTCCCAATGAAAGGCCAATACATTGTCATAACGGCGCAAGGCCGAACTGACCCAAATCAAAACACCGGAATCTCCATCCACAACTGCACCATCAAGCCCACTGCGCAGCTCGCAGCCAGCCCCACCATCAAGAGCTACTTCGGGAGGCCGTGGAAGGAGTACGCTCGAACAGTTTACATGCAGTCGTTTATCGATGCATTTATCGACCCAGCAGGGTGGAATGTGTGGGACGGGACTTTGAATTTGAACACGTCGTATTACGCTGAGTTTAATAACTCGGGGCTGGGATCTGACACTTCGCGGCGGGCTAAATGGGCGGTCGGTTTGATCAACGCCACCGTGGCTAATAACTTCACGGTGTCTCCGTTCTTGGGTGGAGATCAATGGCTACCGCCGACCACCGTGCCGTACACTGGTGGTTTGATATCATAA

Coding sequence (CDS)

ATGGGTTCTTCAAGTGCTATCTCCCTCCTCGGAGTCTTGACTCTCCTCTACCTATTCTCCTTCTCATCCTCCGCCATCCATAGCCCAGTTCCGGCGGCGGATATCTGCCATTCCACAACCTCTCCATCCTTCTGCAAAGCCTTCCTCCCCAACTCCCTTGCCACCGTCCACTCCCATTGCCGCTTCACTCTCCGCCATGCCCTCGCCCATGCACGCTCCTTCCTTGCCCTCGTTGACAGCCACCTCGCCCTCTTCCCCTCCCTCGCTGCCCTCCACGACTGCCGCTCCCTCGCTGCGGCTAACCTCGACTTCCTATCCAACACCTTCCCCACAGTCAACACCACCACCACCACAAACGCCCTTCCCTTCTACAAAGCCGATGAGATGGTGTCGTTGCTTAGCGCAGTCATCACCAATGAAAACACTTGTTACGAAGGGCTGCTGAGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGGCTCGGTTGATAAGGTGTTGAAGGCCATCTCCTTCGATACAAAGCTTTATAGTTTGTACCTTTCTTTGTTCAAAATGGGTTGGGTTTCTAATGATGTCAAGGCTCCTCATGTCCCAAAGATGAAGCATTTTGGGCGTGGAAAGGGGCAGTTGAAGCTCAACATGTCCCCCAAAAACCGTGCCTATTATGAGCGCTTGATGCATAGGAGAAAGCCACCGGCTAGGAGGCTTCTTCAAACCAATTCTAATGAAGATGATGGAATTTTGGTCAATGGTATTGTTGCGGTGGATCCGAATGGAACATATGACTTCACCACCATCAACGCCGCCATTCAGGCAGCTCCCAACAACTCCGTCGCCGCCGATGGATATTTCTTGATCTTCGTCACTGCCGGAGTTTACAACGAGAGCGTGATGGTTCCCAAACACAAAAAGTATGTGTTGATGATTGGAGAAGGCAACAACCAAACCATCATCACCGGGAGAAAAAACGTCCCCGATGGCTCTACCACTTTCAATAGCGGAACCGTCATTGTGGAGGGAACTGGGTTTTTCGGGGTGAACCTGACGATCACCAACACAGCCGGACCCAACAAATTCCAAGCCGTGGCATTGCGAGTGAGTGCCGACATGACGACGTTCTACAGTTGCATATTCGAAGGGTATCAAGACACTCTCTACCCCCACTCTCTTCGACAATTCTATAGAGAGTGCGACATCTACGGAACTATAGACTTTATTTTGGGGGACGCGGCTGTGGTGTTCCAAAACTGCAACATTTACGCCCGCCTCCCAATGAAAGGCCAATACATTGTCATAACGGCGCAAGGCCGAACTGACCCAAATCAAAACACCGGAATCTCCATCCACAACTGCACCATCAAGCCCACTGCGCAGCTCGCAGCCAGCCCCACCATCAAGAGCTACTTCGGGAGGCCGTGGAAGGAGTACGCTCGAACAGTTTACATGCAGTCGTTTATCGATGCATTTATCGACCCAGCAGGGTGGAATGTGTGGGACGGGACTTTGAATTTGAACACGTCGTATTACGCTGAGTTTAATAACTCGGGGCTGGGATCTGACACTTCGCGGCGGGCTAAATGGGCGGTCGGTTTGATCAACGCCACCGTGGCTAATAACTTCACGGTGTCTCCGTTCTTGGGTGGAGATCAATGGCTACCGCCGACCACCGTGCCGTACACTGGTGGTTTGATATCATAA

Protein sequence

MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS
Homology
BLAST of CmoCh02G006080 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 2.3e-147
Identity = 288/576 (50.00%), Postives = 382/576 (66.32%), Query Frame = 0

Query: 9   LLGVLTLLYLFSFSSSAIHSPVPA-------ADICHSTTSPSFCKAFLPNSLATVHSHCR 68
           L+ + TL  L S  S  I + +PA       ++IC     PS+C++ LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 69  FTLRHALAHARSFLALVDSHL------ALFPSLAALHDCRSLAAANLDFLSNTFPTVNTT 128
            +LR +L+ AR F++++D+ L      A   ++ AL DC+ LA+  +D+L ++  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 129 TTTNALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLY 188
            T   L   +A+++ + LSA ITNE TC EGL S+ S +G  SG +         DTKLY
Sbjct: 125 KT---LSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGL-SGDLFN-------DTKLY 184

Query: 189 SLYLSLFKMGWV-----SNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKP 248
            + L+LF  GWV     S  +  P     K FG   G+L L M+ + RA Y  +      
Sbjct: 185 GVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR---- 244

Query: 249 PARRLLQTNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAG 308
             R+LLQ+++   D + V+ IV V  NGT +FTTINAAI AAPN +  ++GYFLI+VTAG
Sbjct: 245 --RKLLQSDA---DAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAG 304

Query: 309 VYNESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTIT 368
           +Y E V VPK+K+YV+MIG+G NQT+ITG ++V DG TTFNS T I+ G  F GVN+TI 
Sbjct: 305 LYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIR 364

Query: 369 NTAGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAA 428
           NTAGP K QAVALR   D++ FYSC FE YQDTLY HSLRQFYRECD+YGT+DFI G+AA
Sbjct: 365 NTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 424

Query: 429 VVFQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFG 488
           VV QNCN+Y R P KGQ   +TAQGRTDPNQNTG +IH CTI+P   LA S  T+K+Y G
Sbjct: 425 VVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLG 484

Query: 489 RPWKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW-AV 548
           RPWKEY+RTV MQ++ID F++P+GWN W G   L+T YYAE+NN+G GSDT+ R  W   
Sbjct: 485 RPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGY 544

Query: 549 GLINATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
            +INAT A+NFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 545 HVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of CmoCh02G006080 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 512.7 bits (1319), Expect = 5.3e-144
Identity = 285/574 (49.65%), Postives = 373/574 (64.98%), Query Frame = 0

Query: 13  LTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLP-NSLATVHSHCRFTLRHALAHA 72
           L+L  LF  S + + S   ++ IC +T  P FCK+  P  S   V  + RF+LR +L  +
Sbjct: 13  LSLQTLFIASQTLLPSN-SSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 72

Query: 73  RSFLALVDSHL----ALF--PSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYK 132
           R F   +D +L    AL    ++ AL DCR LA+   D+L  +F TVN TT++  L F K
Sbjct: 73  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 132

Query: 133 ADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMG 192
           ADE+ +LLSA +TNE TC +G+ ++ S S +    V   L  I+ DTKL+S+ L+LF  G
Sbjct: 133 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGV--ALPLIN-DTKLFSVSLALFTKG 192

Query: 193 WVSNDVKA-------------PHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPA 252
           WV    K               H    +HF    G L L M+   RA YE L       +
Sbjct: 193 WVPKKKKQVASYSWAHPKNTHSHTKPFRHF--RNGALPLKMTEHTRAVYESL-------S 252

Query: 253 RRLLQTNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVY 312
           RR L  + N+ + +LV+ IV V+ NGT +FTTI  A+ +APN +    GYF+I+VT+GVY
Sbjct: 253 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 312

Query: 313 NESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNT 372
            E+V++ K+K+Y++MIG+G N+T++TG +NV DG TTFNS T  V    F  VN+T  NT
Sbjct: 313 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 372

Query: 373 AGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVV 432
           AGP K QAVA+R SAD++ FYSC FE YQDTLY HSLRQFYRECDIYGT+DFI G+AAVV
Sbjct: 373 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 432

Query: 433 FQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFGRP 492
           FQ+CN+Y R PM+ Q+  ITAQGRTDPNQNTGISIHNCTIKP   L +S  T+K+Y GRP
Sbjct: 433 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRP 492

Query: 493 WKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW-AVGL 552
           WKEY+RTV+MQS+ID  ++P GW  W+G   L+T YYAE+NN+G GS T+ R  W    +
Sbjct: 493 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 552

Query: 553 INATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
           IN+T ANNFTV  FL GD W+  + VPY  GL+S
Sbjct: 553 INSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CmoCh02G006080 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.5e-138
Identity = 272/579 (46.98%), Postives = 364/579 (62.87%), Query Frame = 0

Query: 12  VLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRFTLRHALAHA 71
           ++TL +      ++  +   ++ IC +T  P +CK+  P+S   V  +  F++R +L+ +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 72  RSFLALVD------SHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTT---TTTNALP 131
           R F+  VD      +HL+    + AL DCR LA   +D+L  +F TVN T   T+   L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 132 FYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLF 191
           F KAD++ +LLSA +TNE TC EGL ++ S S + +      L  ++ DTKL  + L+LF
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN-DTKLLGVSLALF 187

Query: 192 KMGWVSNDVK-------------APHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRK 251
             GWV    K             + H    + F    G L L M+ K +A YE L  R+ 
Sbjct: 188 TKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLF--RNGALPLKMTEKTKAVYESLSRRKL 247

Query: 252 PPARRLLQTNSNEDDG--ILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFV 311
                   +N + DDG  +L++ IV V  +GT +FT I AA+ AAPNN+  + G+FLI+V
Sbjct: 248 ADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYV 307

Query: 312 TAGVYNESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNL 371
           TAG+Y E + + K+K+Y++MIG+G NQT++TG ++V DG TTFNS T  V    F  VN+
Sbjct: 308 TAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNI 367

Query: 372 TITNTAGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILG 431
           T  NTAGP K QAVALR  AD + FYSC FE YQDTLY HSLRQFYRECD+YGT+DFI G
Sbjct: 368 TFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFG 427

Query: 432 DAAVVFQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKS 491
           +AAVVFQNCN+Y R PM  Q+  ITAQGR+DPNQNTG SI NCTIKP   L +S  T+K+
Sbjct: 428 NAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKT 487

Query: 492 YFGRPWKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW 551
           Y GRPWKEY+RTVYMQS+ID F++P GW  W+G   L+T YYAE+NN+G GS+T+ R  W
Sbjct: 488 YLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTW 547

Query: 552 -AVGLINATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
               +IN+T A NFTV+       W+  T VPYT GLIS
Sbjct: 548 PGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CmoCh02G006080 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.9e-109
Identity = 230/545 (42.20%), Postives = 319/545 (58.53%), Query Frame = 0

Query: 36  CHSTTSPSFCKAFL---PNSLATVHSHCRFTLRHALAHARSFLALVDSHL---------A 95
           C ST  P  C+  L    +S +  + + +FT++  L  A     ++ S+          A
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 96  LFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYKADEMVSLLSAVITNENTC 155
               + A+ DC  L+  ++++L      + T     A      + + SLLS V+TN+ TC
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAA---LVEHVNSLLSGVVTNQQTC 202

Query: 156 YEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMGWVSNDVKAPHVPKMKHFG 215
            +GL+ + SG  +  GS    L      T+LYS+ L L     ++ ++K     K K  G
Sbjct: 203 LDGLVEAKSGFAAAIGSPMGNL------TRLYSISLGLVSHA-LNRNLKRFKASKGKILG 262

Query: 216 RGKGQLKLNMSPKNRAYYERLMHRR--KPPARRLLQTNSNEDDGILVNGIVAVDPNGTYD 275
            G    +  +    +   +   + +  +  +R L +        ILV+  V V P  + +
Sbjct: 263 GGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDN 322

Query: 276 FTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKKYVLMIGEGNNQTIITGRK 335
           FTTI  AI AAPNN+   DGYF+I+   GVY E ++VP +KK ++++G+G N+TIITG  
Sbjct: 323 FTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNH 382

Query: 336 NVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVALRVSADMTTFYSCIFEGYQ 395
           NV DG TT+N  +  V G  F  V++T  NTAGP K QAVALR +A+ ++FY C FEGYQ
Sbjct: 383 NVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQ 442

Query: 396 DTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLPMKGQYIVITAQGRTDPNQ 455
           DTLY HSLRQFYRECDIYGT+DFI G+AA +FQNCNIYAR PM  Q   ITA GR DPNQ
Sbjct: 443 DTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQ 502

Query: 456 NTGISIHNCTIKPTAQLAASP-TIKSYFGRPWKEYARTVYMQSFIDAFIDPAGWNVWDGT 515
           NTGISI NCTIK    LAA P +  ++ GRPWK Y+RTV+MQS+I   + P GW  W+GT
Sbjct: 503 NTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGT 562

Query: 516 LNLNTSYYAEFNNSGLGSDTSRRAKW-AVGLINATVANNFTVSPFLGGDQWLPPTTVPYT 565
           + L+T YY E++N G G++T++R +W    L+N   A NFTV  F  GD WLP T +P+ 
Sbjct: 563 IGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFY 617

BLAST of CmoCh02G006080 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 5.2e-107
Identity = 227/552 (41.12%), Postives = 315/552 (57.07%), Query Frame = 0

Query: 28  SPVPAADICHSTTSPSFCKAFL---PNSLATVHSHCRFTLRHALAHARSFLALV------ 87
           SP+P    C ST  P  C+  L    +S +  + + +FT++  L  AR    ++      
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 88  ---DSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYKADEMVSLLSA 147
              D   +    ++A+ DC  LA  ++++L      +       A      D + SLL  
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAA---LVDRVTSLLGG 199

Query: 148 VITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMGWVSNDVKAPH 207
           V+TN+ TC +GL+ + SG  +  G+       +   T+LYS+ L L     ++ ++K   
Sbjct: 200 VVTNQQTCLDGLVDAKSGFATAIGT------PLGNLTRLYSVSLGLVSHA-LNRNLKRYK 259

Query: 208 VPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRR--KPPARRLLQTNSNEDDGILVNGIVA 267
             K K FG G   ++  +    +   +     +  +   R L +        ILV   V 
Sbjct: 260 GSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVT 319

Query: 268 VDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKKYVLMIGEGNN 327
           V P  T +F TI  A+ AAPN++    GYF+I+  AG+Y E V++   K+ +++IG+G N
Sbjct: 320 VGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGIN 379

Query: 328 QTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVALRVSADMTTFY 387
           +TII+G  +  DG TT+NS T  V G  F  V++T  NTAGP K QAVA+R +AD +TFY
Sbjct: 380 KTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFY 439

Query: 388 SCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLPMKGQYIVITA 447
            C FEGYQDTLY HSLRQFYRECDIYGTIDFI G+AA +FQNCNIYAR PM  Q   +TA
Sbjct: 440 RCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTA 499

Query: 448 QGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFGRPWKEYARTVYMQSFIDAFIDPA 507
            GRTDPNQ TGISI NCTI     LAA P +  ++ GRPWK Y+RTVY+QS+I   + P 
Sbjct: 500 HGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPV 559

Query: 508 GWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWA-VGLINATVANNFTVSPFLGGDQWL 564
           GW  W+GT  L+T  Y E++N G G+DTS+R +W+   L+N   A NFTV  F  GD WL
Sbjct: 560 GWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWL 619

BLAST of CmoCh02G006080 vs. ExPASy TrEMBL
Match: A0A6J1G612 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451102 PE=3 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120
           RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA
Sbjct: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120

Query: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180
           LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS
Sbjct: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180

Query: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240
           LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS
Sbjct: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240

Query: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300
           NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK
Sbjct: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300

Query: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360
           HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA
Sbjct: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360

Query: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420
           VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA
Sbjct: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420

Query: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480
           RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY
Sbjct: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480

Query: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540
           MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT
Sbjct: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540

Query: 541 VSPFLGGDQWLPPTTVPYTGGLIS 565
           VSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 VSPFLGGDQWLPPTTVPYTGGLIS 564

BLAST of CmoCh02G006080 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 1068.9 bits (2763), Expect = 7.1e-309
Identity = 541/566 (95.58%), Postives = 545/566 (96.29%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAI LL  LTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVN--TTTTT 120
           RFTLRHALAHAR+FLALVD+HL LFPSLAALHDCRSLAAANLDFLSNTFPTVN  TTTTT
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 NALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLY 180
           NALPFYKA+EMVSLLSAVITNENTCYEGL+S    SGS SG VDKVLKAISFDTKLYSLY
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMS----SGSASGLVDKVLKAISFDTKLYSLY 180

Query: 181 LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQT 240
           LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHR KPPARRLLQT
Sbjct: 181 LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQT 240

Query: 241 NSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMV 300
           NSNEDDGILVNGIVAVD NGTYDFTTINAAIQAAPNNSVAADGYFLI VTAGVYNESVMV
Sbjct: 241 NSNEDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMV 300

Query: 301 PKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKF 360
           PKHKKYVLMIGEGNNQTIITG KNV DGSTTFNSGT IVEGTGFFGVNLTITNTAGPNKF
Sbjct: 301 PKHKKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKF 360

Query: 361 QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI 420
           QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI
Sbjct: 361 QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI 420

Query: 421 YARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART 480
           YARLPMKGQYIVITAQGRTD NQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART
Sbjct: 421 YARLPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART 480

Query: 481 VYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANN 540
           VYMQSFIDAFIDP GWNVWDGTLNLNTSYYAEFNNSG GSDTSRRAKWAVGLINATVANN
Sbjct: 481 VYMQSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANN 540

Query: 541 FTVSPFLGGDQWLPPTTVPYTGGLIS 565
           FTVSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 FTVSPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of CmoCh02G006080 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 9.8e-242
Identity = 432/562 (76.87%), Postives = 475/562 (84.52%), Query Frame = 0

Query: 3   SSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRF 62
           SS+ ISLL   +LL+LF F+SS   S VP  ++C S TSPSFCK+FLP+S A+VHSHCR+
Sbjct: 4   SSNPISLLTFFSLLFLFLFTSS---SAVPTVEVCQSATSPSFCKSFLPSSPASVHSHCRY 63

Query: 63  TLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALP 122
           TL HAL  AR+F+ LVD  L L PS +ALHDCRSLA ANLDF+S+T  TVN  TTTNALP
Sbjct: 64  TLHHALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVN--TTTNALP 123

Query: 123 FYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLF 182
           FY+ADEM+SLLSAVITN +TC++GL      +GSG G VDKV+KAISFD +LYSLYLSLF
Sbjct: 124 FYQADEMISLLSAVITNVDTCHDGL------AGSGLGLVDKVVKAISFDRQLYSLYLSLF 183

Query: 183 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNSNE 242
           KMGWVS  +KAP +PK  HFG G G LKL MSPK+RAYYERLMH +KP ARRLLQTN N 
Sbjct: 184 KMGWVSKTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTN-NA 243

Query: 243 DDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHK 302
           DDGILVNG+VAVD NG YDFTTIN AI AAPN S AA+GYFLIFVTAG+Y ESV+VPK K
Sbjct: 244 DDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSK 303

Query: 303 KYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVA 362
           KYVLMIGEGNN+TIITG KNV DGSTTFNS TVIVEGTGFFG NLTITNTAG  K QAVA
Sbjct: 304 KYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVA 363

Query: 363 LRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARL 422
           LRVSADMTTFYSCIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIYARL
Sbjct: 364 LRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARL 423

Query: 423 PMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQ 482
           P+ GQ+  +TAQGRTD NQNTGI IH CTIKPT  LAASPT KSY GRPWKE++RTVYMQ
Sbjct: 424 PLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQ 483

Query: 483 SFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFTVS 542
           SFIDAFIDPAGW  WDGTLNLNTSYYAEF+NSG GSDTSRRA+WAVG+INATVAN FTVS
Sbjct: 484 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVS 543

Query: 543 PFLGGDQWLPPTTVPYTGGLIS 565
             L GDQWLPPT VPY+GGLIS
Sbjct: 544 EILAGDQWLPPTAVPYSGGLIS 553

BLAST of CmoCh02G006080 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.9e-241
Identity = 431/562 (76.69%), Postives = 478/562 (85.05%), Query Frame = 0

Query: 3   SSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRF 62
           SS+ ISLL   +LL+LF+ SS+     VP  ++C S TSPSFCK+FLP+S A+VHSHCR+
Sbjct: 4   SSNPISLLLFFSLLFLFTSSSA-----VPTVEVCQSATSPSFCKSFLPSSPASVHSHCRY 63

Query: 63  TLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALP 122
           TL HAL  AR+F+ LVD  L LFPSL+ALHDCRSLA ANLDF+S+T  TVN  TTTNALP
Sbjct: 64  TLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVN--TTTNALP 123

Query: 123 FYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLF 182
           FY+ADEM+SLLSAVITN +TC++GL    +GSG G G VDKV+KAISFD +LYSLYLSLF
Sbjct: 124 FYQADEMISLLSAVITNIDTCHDGL----AGSGLGLGFVDKVVKAISFDRQLYSLYLSLF 183

Query: 183 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNSNE 242
           KMGWVS ++KAP +PK  HFG GKG LKL MSPK+RAYYERL+H +KP ARRLLQTN   
Sbjct: 184 KMGWVSKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAARRLLQTN-YA 243

Query: 243 DDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHK 302
           DDGILVNG+VAVD NG YDFTTIN AI AAPN S AA+GYFLIFVTAG+Y ESV+VPK K
Sbjct: 244 DDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSK 303

Query: 303 KYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVA 362
           KYVLMIGEGNN+TIITG KNV DGSTTFNS TVIVEGTGFFG NLTITNTAG  K QAVA
Sbjct: 304 KYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVA 363

Query: 363 LRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARL 422
           LRVSADMTTFYSCIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIYARL
Sbjct: 364 LRVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARL 423

Query: 423 PMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQ 482
           P+ GQ+  +TAQGRTD NQNTGI IH CTIKPT  LAASPT KSY GRPWKE++RTVYMQ
Sbjct: 424 PLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQ 483

Query: 483 SFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFTVS 542
           SFIDAFIDPAGW  WDGTLNLNTSYYAEF+NSG GSDTS+RA+WAVG+INATVAN FTVS
Sbjct: 484 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVS 543

Query: 543 PFLGGDQWLPPTTVPYTGGLIS 565
             L GDQWLPPT VPY+GGLIS
Sbjct: 544 EILAGDQWLPPTAVPYSGGLIS 553

BLAST of CmoCh02G006080 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.6e-239
Identity = 427/565 (75.58%), Postives = 475/565 (84.07%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           M SS+ IS+L  LTLL+  S SSSA    VP A+IC STTSP FC  FLPNS A+VHSHC
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA----VPTAEICQSTTSPPFCNFFLPNSPASVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120
           RFTL HAL HAR+FL+LV++HL LFPSL+ LHDCRSLA ANLDFLS TF  VN+TTTT  
Sbjct: 61  RFTLHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTTT-L 120

Query: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180
           LP+Y A E ++L+SAVITNE+TCYEGL    +G  S  G VDKVL+AISFD KLYSLYLS
Sbjct: 121 LPYYDAHEKLTLMSAVITNEDTCYEGL----AGLDSAVGLVDKVLEAISFDKKLYSLYLS 180

Query: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPP-ARRLLQTN 240
           LFKMGWVS D+KAP +PKMKHFG GKGQLKL MSPK+RAYYERL+HR KPP ARRLLQTN
Sbjct: 181 LFKMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQTN 240

Query: 241 SNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVP 300
             +DDGI++NGIV VD NGTYDFT I AAI AAPN +  A GYFLIFV AG+YNE+V++P
Sbjct: 241 -YQDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIP 300

Query: 301 KHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQ 360
           K K+YVL+IGEGNNQTIITG KNV DGSTTFNS TV VEGTGF GVNLTI+NTAG  K Q
Sbjct: 301 KEKRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQ 360

Query: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420
           AVALRVSAD  T Y+CIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIY
Sbjct: 361 AVALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIY 420

Query: 421 ARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV 480
           ARLP+ GQ+  +TAQGRTDPNQNTG SIHNCTIK T +LAASP  KSY GRPWK+Y+RTV
Sbjct: 421 ARLPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTV 480

Query: 481 YMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNF 540
           YMQSFID+FIDPAGW  WDGT+NLNTSYYAEFNNSG GSDTS+R  WAVG+INATVAN+F
Sbjct: 481 YMQSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSF 540

Query: 541 TVSPFLGGDQWLPPTTVPYTGGLIS 565
           TVS FL GD+WLPPT VPYTGGLIS
Sbjct: 541 TVSQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of CmoCh02G006080 vs. NCBI nr
Match: XP_022947145.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita moschata])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120
           RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA
Sbjct: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNA 120

Query: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180
           LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS
Sbjct: 121 LPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLS 180

Query: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240
           LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS
Sbjct: 181 LFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNS 240

Query: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300
           NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK
Sbjct: 241 NEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPK 300

Query: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360
           HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA
Sbjct: 301 HKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQA 360

Query: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420
           VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA
Sbjct: 361 VALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYA 420

Query: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480
           RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY
Sbjct: 421 RLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVY 480

Query: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540
           MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT
Sbjct: 481 MQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFT 540

Query: 541 VSPFLGGDQWLPPTTVPYTGGLIS 565
           VSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 VSPFLGGDQWLPPTTVPYTGGLIS 564

BLAST of CmoCh02G006080 vs. NCBI nr
Match: KAG6605296.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1106.3 bits (2860), Expect = 0.0e+00
Identity = 558/567 (98.41%), Postives = 561/567 (98.94%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVN---TTTT 120
           RFTLRHALAHARSFLALV++HLALFPSLAALHDCRSLAAANLDFLSNTFPTVN   TTTT
Sbjct: 61  RFTLRHALAHARSFLALVNTHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 TNALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSL 180
           TNALPFYKADEMVSLLSAVITNENTCYEGLLS  SGSGSGSGSVDKVLKAISFDTKLYSL
Sbjct: 121 TNALPFYKADEMVSLLSAVITNENTCYEGLLS--SGSGSGSGSVDKVLKAISFDTKLYSL 180

Query: 181 YLSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQ 240
           YLSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQ
Sbjct: 181 YLSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQ 240

Query: 241 TNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVM 300
           TNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVM
Sbjct: 241 TNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVM 300

Query: 301 VPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNK 360
           VPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNK
Sbjct: 301 VPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNK 360

Query: 361 FQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCN 420
           FQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCN
Sbjct: 361 FQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCN 420

Query: 421 IYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYAR 480
           IYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAA+PTIKSYFGRPWKEYAR
Sbjct: 421 IYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAATPTIKSYFGRPWKEYAR 480

Query: 481 TVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVAN 540
           TVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSG GSDTSRRAKWAVGLINATVAN
Sbjct: 481 TVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVAN 540

Query: 541 NFTVSPFLGGDQWLPPTTVPYTGGLIS 565
           NFTVSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 NFTVSPFLGGDQWLPPTTVPYTGGLIS 565

BLAST of CmoCh02G006080 vs. NCBI nr
Match: XP_023534721.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 554/565 (98.05%), Postives = 555/565 (98.23%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAISLL  LTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAISLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVN-TTTTTN 120
           RFTLRHALAHARSFLALVD+HLALFPSLAALHDCRSLAAANLDFLSNTFPTVN TTTTTN
Sbjct: 61  RFTLRHALAHARSFLALVDTHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTN 120

Query: 121 ALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYL 180
           ALPFYKADEMVSLLSAVITNENTCYEGLLS      SGSGSVDKVLKAISFDTKLYSLYL
Sbjct: 121 ALPFYKADEMVSLLSAVITNENTCYEGLLS------SGSGSVDKVLKAISFDTKLYSLYL 180

Query: 181 SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTN 240
           SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTN
Sbjct: 181 SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTN 240

Query: 241 SNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVP 300
           SNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVP
Sbjct: 241 SNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVP 300

Query: 301 KHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQ 360
           KHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQ
Sbjct: 301 KHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQ 360

Query: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420
           AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY
Sbjct: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420

Query: 421 ARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV 480
           ARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV
Sbjct: 421 ARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV 480

Query: 481 YMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNF 540
           YMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSG GSDTSRRAKWAVGLINATVANNF
Sbjct: 481 YMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNF 540

Query: 541 TVSPFLGGDQWLPPTTVPYTGGLIS 565
           TVSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 TVSPFLGGDQWLPPTTVPYTGGLIS 559

BLAST of CmoCh02G006080 vs. NCBI nr
Match: XP_023007148.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita maxima])

HSP 1 Score: 1068.9 bits (2763), Expect = 1.5e-308
Identity = 541/566 (95.58%), Postives = 545/566 (96.29%), Query Frame = 0

Query: 1   MGSSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60
           MGSSSAI LL  LTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC
Sbjct: 1   MGSSSAIPLLTFLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHC 60

Query: 61  RFTLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVN--TTTTT 120
           RFTLRHALAHAR+FLALVD+HL LFPSLAALHDCRSLAAANLDFLSNTFPTVN  TTTTT
Sbjct: 61  RFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTTT 120

Query: 121 NALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLY 180
           NALPFYKA+EMVSLLSAVITNENTCYEGL+S    SGS SG VDKVLKAISFDTKLYSLY
Sbjct: 121 NALPFYKAEEMVSLLSAVITNENTCYEGLMS----SGSASGLVDKVLKAISFDTKLYSLY 180

Query: 181 LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQT 240
           LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHR KPPARRLLQT
Sbjct: 181 LSLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQT 240

Query: 241 NSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMV 300
           NSNEDDGILVNGIVAVD NGTYDFTTINAAIQAAPNNSVAADGYFLI VTAGVYNESVMV
Sbjct: 241 NSNEDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMV 300

Query: 301 PKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKF 360
           PKHKKYVLMIGEGNNQTIITG KNV DGSTTFNSGT IVEGTGFFGVNLTITNTAGPNKF
Sbjct: 301 PKHKKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKF 360

Query: 361 QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI 420
           QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI
Sbjct: 361 QAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNI 420

Query: 421 YARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART 480
           YARLPMKGQYIVITAQGRTD NQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART
Sbjct: 421 YARLPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYART 480

Query: 481 VYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANN 540
           VYMQSFIDAFIDP GWNVWDGTLNLNTSYYAEFNNSG GSDTSRRAKWAVGLINATVANN
Sbjct: 481 VYMQSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANN 540

Query: 541 FTVSPFLGGDQWLPPTTVPYTGGLIS 565
           FTVSPFLGGDQWLPPTTVPYTGGLIS
Sbjct: 541 FTVSPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of CmoCh02G006080 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847.8 bits (2189), Expect = 5.3e-242
Identity = 433/562 (77.05%), Postives = 479/562 (85.23%), Query Frame = 0

Query: 3   SSSAISLLGVLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRF 62
           SS+ ISLL   +LL+LF+ SS+     VP  ++C S TSPSFCK+FLP+S A+VHSHCR+
Sbjct: 4   SSNPISLLLFFSLLFLFTSSSA-----VPTVEVCQSATSPSFCKSFLPSSPASVHSHCRY 63

Query: 63  TLRHALAHARSFLALVDSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALP 122
           TL HAL  AR+F+ LVD  L LFPSL+ALHDCRSLA ANLDF+S+T  TVN  TTTNALP
Sbjct: 64  TLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVN--TTTNALP 123

Query: 123 FYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLF 182
           FY+ADEM+SLLSAVITN +TC++GL  +GSGSGSG G VDKV+KAISFD +LYSLYLSLF
Sbjct: 124 FYQADEMISLLSAVITNVDTCHDGL--AGSGSGSGLGLVDKVVKAISFDRQLYSLYLSLF 183

Query: 183 KMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPARRLLQTNSNE 242
           KMGWVS ++KAP +PK  HFG G G LKL MSPK+RAYYERL+H +KP ARRLLQTN   
Sbjct: 184 KMGWVSKNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKPAARRLLQTN-YA 243

Query: 243 DDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHK 302
           DDGILVNG+VAVD NG YDFTTIN AI AAPN S AA+GYFLIFVTAG+Y ESV+VPK K
Sbjct: 244 DDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSK 303

Query: 303 KYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVA 362
           KYVLMIGEGNN+TIITG KNV DGSTTFNS TVIVEGTGFFG NLTITNTAG  K QAVA
Sbjct: 304 KYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVA 363

Query: 363 LRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARL 422
           LRVSADMTTFYSCIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVV QNCNIYARL
Sbjct: 364 LRVSADMTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARL 423

Query: 423 PMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTVYMQ 482
           P+ GQ+  +TAQGRTD NQNTGI IH CTIKPT  LAASP  KSY GRPWKE++RTVYMQ
Sbjct: 424 PLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYMQ 483

Query: 483 SFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWAVGLINATVANNFTVS 542
           SFIDAFIDPAGW  WDGTLNLNTSYYAEF+NSG GSDTSRRA+WAVG+INATVAN FTVS
Sbjct: 484 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVS 543

Query: 543 PFLGGDQWLPPTTVPYTGGLIS 565
             L GDQWLPPT VPY+GGLIS
Sbjct: 544 EILAGDQWLPPTAVPYSGGLIS 555

BLAST of CmoCh02G006080 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 523.9 bits (1348), Expect = 1.6e-148
Identity = 288/576 (50.00%), Postives = 382/576 (66.32%), Query Frame = 0

Query: 9   LLGVLTLLYLFSFSSSAIHSPVPA-------ADICHSTTSPSFCKAFLPNSLATVHSHCR 68
           L+ + TL  L S  S  I + +PA       ++IC     PS+C++ LPN    ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 69  FTLRHALAHARSFLALVDSHL------ALFPSLAALHDCRSLAAANLDFLSNTFPTVNTT 128
            +LR +L+ AR F++++D+ L      A   ++ AL DC+ LA+  +D+L ++  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 129 TTTNALPFYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLY 188
            T   L   +A+++ + LSA ITNE TC EGL S+ S +G  SG +         DTKLY
Sbjct: 125 KT---LSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGL-SGDLFN-------DTKLY 184

Query: 189 SLYLSLFKMGWV-----SNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKP 248
            + L+LF  GWV     S  +  P     K FG   G+L L M+ + RA Y  +      
Sbjct: 185 GVSLALFSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTR---- 244

Query: 249 PARRLLQTNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAG 308
             R+LLQ+++   D + V+ IV V  NGT +FTTINAAI AAPN +  ++GYFLI+VTAG
Sbjct: 245 --RKLLQSDA---DAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAG 304

Query: 309 VYNESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTIT 368
           +Y E V VPK+K+YV+MIG+G NQT+ITG ++V DG TTFNS T I+ G  F GVN+TI 
Sbjct: 305 LYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIR 364

Query: 369 NTAGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAA 428
           NTAGP K QAVALR   D++ FYSC FE YQDTLY HSLRQFYRECD+YGT+DFI G+AA
Sbjct: 365 NTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAA 424

Query: 429 VVFQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFG 488
           VV QNCN+Y R P KGQ   +TAQGRTDPNQNTG +IH CTI+P   LA S  T+K+Y G
Sbjct: 425 VVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLG 484

Query: 489 RPWKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW-AV 548
           RPWKEY+RTV MQ++ID F++P+GWN W G   L+T YYAE+NN+G GSDT+ R  W   
Sbjct: 485 RPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGY 544

Query: 549 GLINATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
            +INAT A+NFTV+ FL G+ W+  T VP+ GGLI+
Sbjct: 545 HVINATDASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of CmoCh02G006080 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 512.7 bits (1319), Expect = 3.8e-145
Identity = 285/574 (49.65%), Postives = 373/574 (64.98%), Query Frame = 0

Query: 13  LTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLP-NSLATVHSHCRFTLRHALAHA 72
           L+L  LF  S + + S   ++ IC +T  P FCK+  P  S   V  + RF+LR +L  +
Sbjct: 13  LSLQTLFIASQTLLPSN-SSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKSLTQS 72

Query: 73  RSFLALVDSHL----ALF--PSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYK 132
           R F   +D +L    AL    ++ AL DCR LA+   D+L  +F TVN TT++  L F K
Sbjct: 73  RKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTLSFSK 132

Query: 133 ADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMG 192
           ADE+ +LLSA +TNE TC +G+ ++ S S +    V   L  I+ DTKL+S+ L+LF  G
Sbjct: 133 ADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGV--ALPLIN-DTKLFSVSLALFTKG 192

Query: 193 WVSNDVKA-------------PHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRKPPA 252
           WV    K               H    +HF    G L L M+   RA YE L       +
Sbjct: 193 WVPKKKKQVASYSWAHPKNTHSHTKPFRHF--RNGALPLKMTEHTRAVYESL-------S 252

Query: 253 RRLLQTNSNEDDGILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVY 312
           RR L  + N+ + +LV+ IV V+ NGT +FTTI  A+ +APN +    GYF+I+VT+GVY
Sbjct: 253 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 312

Query: 313 NESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNT 372
            E+V++ K+K+Y++MIG+G N+T++TG +NV DG TTFNS T  V    F  VN+T  NT
Sbjct: 313 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 372

Query: 373 AGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVV 432
           AGP K QAVA+R SAD++ FYSC FE YQDTLY HSLRQFYRECDIYGT+DFI G+AAVV
Sbjct: 373 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 432

Query: 433 FQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFGRP 492
           FQ+CN+Y R PM+ Q+  ITAQGRTDPNQNTGISIHNCTIKP   L +S  T+K+Y GRP
Sbjct: 433 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRP 492

Query: 493 WKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW-AVGL 552
           WKEY+RTV+MQS+ID  ++P GW  W+G   L+T YYAE+NN+G GS T+ R  W    +
Sbjct: 493 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 552

Query: 553 INATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
           IN+T ANNFTV  FL GD W+  + VPY  GL+S
Sbjct: 553 INSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of CmoCh02G006080 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 494.6 bits (1272), Expect = 1.1e-139
Identity = 272/579 (46.98%), Postives = 364/579 (62.87%), Query Frame = 0

Query: 12  VLTLLYLFSFSSSAIHSPVPAADICHSTTSPSFCKAFLPNSLATVHSHCRFTLRHALAHA 71
           ++TL +      ++  +   ++ IC +T  P +CK+  P+S   V  +  F++R +L+ +
Sbjct: 8   LITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSLSQS 67

Query: 72  RSFLALVD------SHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTT---TTTNALP 131
           R F+  VD      +HL+    + AL DCR LA   +D+L  +F TVN T   T+   L 
Sbjct: 68  RKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLS 127

Query: 132 FYKADEMVSLLSAVITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLF 191
           F KAD++ +LLSA +TNE TC EGL ++ S S + +      L  ++ DTKL  + L+LF
Sbjct: 128 FPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN-DTKLLGVSLALF 187

Query: 192 KMGWVSNDVK-------------APHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRRK 251
             GWV    K             + H    + F    G L L M+ K +A YE L  R+ 
Sbjct: 188 TKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLF--RNGALPLKMTEKTKAVYESLSRRKL 247

Query: 252 PPARRLLQTNSNEDDG--ILVNGIVAVDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFV 311
                   +N + DDG  +L++ IV V  +GT +FT I AA+ AAPNN+  + G+FLI+V
Sbjct: 248 ADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYV 307

Query: 312 TAGVYNESVMVPKHKKYVLMIGEGNNQTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNL 371
           TAG+Y E + + K+K+Y++MIG+G NQT++TG ++V DG TTFNS T  V    F  VN+
Sbjct: 308 TAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNI 367

Query: 372 TITNTAGPNKFQAVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILG 431
           T  NTAGP K QAVALR  AD + FYSC FE YQDTLY HSLRQFYRECD+YGT+DFI G
Sbjct: 368 TFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFG 427

Query: 432 DAAVVFQNCNIYARLPMKGQYIVITAQGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKS 491
           +AAVVFQNCN+Y R PM  Q+  ITAQGR+DPNQNTG SI NCTIKP   L +S  T+K+
Sbjct: 428 NAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKT 487

Query: 492 YFGRPWKEYARTVYMQSFIDAFIDPAGWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKW 551
           Y GRPWKEY+RTVYMQS+ID F++P GW  W+G   L+T YYAE+NN+G GS+T+ R  W
Sbjct: 488 YLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTW 547

Query: 552 -AVGLINATVANNFTVSPFLGGDQWLPPTTVPYTGGLIS 565
               +IN+T A NFTV+       W+  T VPYT GLIS
Sbjct: 548 PGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of CmoCh02G006080 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-110
Identity = 230/545 (42.20%), Postives = 319/545 (58.53%), Query Frame = 0

Query: 36  CHSTTSPSFCKAFL---PNSLATVHSHCRFTLRHALAHARSFLALVDSHL---------A 95
           C ST  P  C+  L    +S +  + + +FT++  L  A     ++ S+          A
Sbjct: 83  CKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYARRVESKPGSA 142

Query: 96  LFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYKADEMVSLLSAVITNENTC 155
               + A+ DC  L+  ++++L      + T     A      + + SLLS V+TN+ TC
Sbjct: 143 TAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAA---LVEHVNSLLSGVVTNQQTC 202

Query: 156 YEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMGWVSNDVKAPHVPKMKHFG 215
            +GL+ + SG  +  GS    L      T+LYS+ L L     ++ ++K     K K  G
Sbjct: 203 LDGLVEAKSGFAAAIGSPMGNL------TRLYSISLGLVSHA-LNRNLKRFKASKGKILG 262

Query: 216 RGKGQLKLNMSPKNRAYYERLMHRR--KPPARRLLQTNSNEDDGILVNGIVAVDPNGTYD 275
            G    +  +    +   +   + +  +  +R L +        ILV+  V V P  + +
Sbjct: 263 GGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKSDN 322

Query: 276 FTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKKYVLMIGEGNNQTIITGRK 335
           FTTI  AI AAPNN+   DGYF+I+   GVY E ++VP +KK ++++G+G N+TIITG  
Sbjct: 323 FTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITGNH 382

Query: 336 NVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVALRVSADMTTFYSCIFEGYQ 395
           NV DG TT+N  +  V G  F  V++T  NTAGP K QAVALR +A+ ++FY C FEGYQ
Sbjct: 383 NVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEGYQ 442

Query: 396 DTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLPMKGQYIVITAQGRTDPNQ 455
           DTLY HSLRQFYRECDIYGT+DFI G+AA +FQNCNIYAR PM  Q   ITA GR DPNQ
Sbjct: 443 DTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDPNQ 502

Query: 456 NTGISIHNCTIKPTAQLAASP-TIKSYFGRPWKEYARTVYMQSFIDAFIDPAGWNVWDGT 515
           NTGISI NCTIK    LAA P +  ++ GRPWK Y+RTV+MQS+I   + P GW  W+GT
Sbjct: 503 NTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWNGT 562

Query: 516 LNLNTSYYAEFNNSGLGSDTSRRAKW-AVGLINATVANNFTVSPFLGGDQWLPPTTVPYT 565
           + L+T YY E++N G G++T++R +W    L+N   A NFTV  F  GD WLP T +P+ 
Sbjct: 563 IGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIPFY 617

BLAST of CmoCh02G006080 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 389.8 bits (1000), Expect = 3.7e-108
Identity = 227/552 (41.12%), Postives = 315/552 (57.07%), Query Frame = 0

Query: 28  SPVPAADICHSTTSPSFCKAFL---PNSLATVHSHCRFTLRHALAHARSFLALV------ 87
           SP+P    C ST  P  C+  L    +S +  + + +FT++  L  AR    ++      
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 88  ---DSHLALFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTTTNALPFYKADEMVSLLSA 147
              D   +    ++A+ DC  LA  ++++L      +       A      D + SLL  
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAA---LVDRVTSLLGG 199

Query: 148 VITNENTCYEGLLSSGSGSGSGSGSVDKVLKAISFDTKLYSLYLSLFKMGWVSNDVKAPH 207
           V+TN+ TC +GL+ + SG  +  G+       +   T+LYS+ L L     ++ ++K   
Sbjct: 200 VVTNQQTCLDGLVDAKSGFATAIGT------PLGNLTRLYSVSLGLVSHA-LNRNLKRYK 259

Query: 208 VPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRR--KPPARRLLQTNSNEDDGILVNGIVA 267
             K K FG G   ++  +    +   +     +  +   R L +        ILV   V 
Sbjct: 260 GSKGKIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVT 319

Query: 268 VDPNGTYDFTTINAAIQAAPNNSVAADGYFLIFVTAGVYNESVMVPKHKKYVLMIGEGNN 327
           V P  T +F TI  A+ AAPN++    GYF+I+  AG+Y E V++   K+ +++IG+G N
Sbjct: 320 VGPYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGIN 379

Query: 328 QTIITGRKNVPDGSTTFNSGTVIVEGTGFFGVNLTITNTAGPNKFQAVALRVSADMTTFY 387
           +TII+G  +  DG TT+NS T  V G  F  V++T  NTAGP K QAVA+R +AD +TFY
Sbjct: 380 KTIISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFY 439

Query: 388 SCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIYARLPMKGQYIVITA 447
            C FEGYQDTLY HSLRQFYRECDIYGTIDFI G+AA +FQNCNIYAR PM  Q   +TA
Sbjct: 440 RCSFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTA 499

Query: 448 QGRTDPNQNTGISIHNCTIKPTAQLAASP-TIKSYFGRPWKEYARTVYMQSFIDAFIDPA 507
            GRTDPNQ TGISI NCTI     LAA P +  ++ GRPWK Y+RTVY+QS+I   + P 
Sbjct: 500 HGRTDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPV 559

Query: 508 GWNVWDGTLNLNTSYYAEFNNSGLGSDTSRRAKWA-VGLINATVANNFTVSPFLGGDQWL 564
           GW  W+GT  L+T  Y E++N G G+DTS+R +W+   L+N   A NFTV  F  GD WL
Sbjct: 560 GWLEWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWL 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222562.3e-14750.00Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK75.3e-14449.65Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX41.5e-13846.98Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q94CB11.9e-10942.20Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF775.2e-10741.12Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1G6120.0e+00100.00Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451102 PE=3 SV=1[more]
A0A6J1L6X07.1e-30995.58Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1[more]
A0A6J1I6B89.8e-24276.87Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1[more]
A0A6J1EJM84.9e-24176.69Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1[more]
A0A5A7TRW11.6e-23975.58Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G00221... [more]
Match NameE-valueIdentityDescription
XP_022947145.10.0e+00100.00probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita moschata][more]
KAG6605296.10.0e+0098.41putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
XP_023534721.10.0e+0098.05probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita pepo subsp. pepo][more]
XP_023007148.11.5e-30895.58probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita maxima][more]
KAG6571391.15.3e-24277.05putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
AT2G47550.11.6e-14850.00Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.13.8e-14549.65Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.11.1e-13946.98Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.21.4e-11042.20Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.13.7e-10841.12Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..181
e-value: 1.8E-5
score: 34.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 34..154
e-value: 2.5E-11
score: 44.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 253..548
e-value: 6.9E-119
score: 396.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 32..184
e-value: 1.6E-12
score: 49.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 34..184
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 248..562
e-value: 8.8E-111
score: 372.3
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 15..562
NoneNo IPR availablePANTHERPTHR31707:SF275PECTINESTERASEcoord: 15..562
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 35..186
e-value: 1.94476E-17
score: 77.4785
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 248..561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G006080.1CmoCh02G006080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity