CmoCh02G006060 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh02G006060 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-90
Identity = 164/332 (49.40%), Postives = 223/332 (67.17%), Query Frame = 0

Query: 39  AFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLP 98
           A F+FGDS  D GNNN+INT  +F+ANF PYG+++F  PT RF+DGR IPDF+AEYA LP
Sbjct: 33  ALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIAEYASLP 92

Query: 99  LIPPYLDPHTNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNAS 158
           +IP YL+P+ +F  +G NFAS+G+GAL  SH G A+GLQTQ+++F  ++   R+ LG+  
Sbjct: 93  IIPAYLEPNNDF-THGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQNLGDIK 152

Query: 159 AQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGR 218
           ++ L+S++++LF+ GGNDY +P+            QE++V++V+GN+T  +K +Y+ GGR
Sbjct: 153 SRQLLSDAVYLFSCGGNDYQSPY--------YPYTQEQYVDIVIGNMTNVIKGIYEKGGR 212

Query: 219 KFGFMTVPPLGHMPSSRIKRSVQFFE-EASSITRLHNQLLPDALQKLGIQLEGFKYAFAD 278
           KFG + VP +G  P  R K+       E   +TRLHNQ     L++L  QLEGF YA  D
Sbjct: 213 KFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGFVYAKFD 272

Query: 279 THTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSF 338
             T +L R+ NP KYGFK  E+ACCGSG F G Y+CGR  E   F  CDN  +Y FFD F
Sbjct: 273 LSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRIKE---FGLCDNATEYFFFDPF 332

Query: 339 HPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
           HP E   +Q AE+ W GD+ + +PYN K LFE
Sbjct: 333 HPNELASRQFAEMFWDGDSMVTQPYNLKALFE 352

BLAST of CmoCh02G006060 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 8.9e-87
Identity = 169/340 (49.71%), Postives = 217/340 (63.82%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYI+T   F++N+ PYG+T F  PT R +DGRTIPDF+AEYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 96  GLPLIPPYLDPHT--NFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LPLIP YL P    N +  GV+FAS+G+GAL  +  G  I L++Q+  FKKV K LR  
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG A  + +IS +++LF+ G NDY  PF  +  IF  SS QE +V+ VVGN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQ-SSPQEIYVDFVVGNTTAVIKEVY 215

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
           K GGRKFGF+ +      P+S I    +    F+  + +  LHN+ L   L++L  +L G
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNC-GRNVEGLPFTHCDNLE 335
           FKYA  D HT L  R+ NP KYGFK  + ACCG+G  RGI  C GR      +  C+ + 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 336 DYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
           DY+FFD FH TEK  +Q+AELIWSG   + KPYN + LFE
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of CmoCh02G006060 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-84
Identity = 165/341 (48.39%), Postives = 220/341 (64.52%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYI+T +  ++N+ PYG+T F  PT R +DGR IPDF+AEYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 96  GLPLIPPYLDPH--TNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LPLIPP L P    + +  GVNFAS G+GAL  +  G  I L+TQ+  FKKV + LR K
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG+A  + +IS +++LF+ G NDY  PF  +  +F  S + E++V+ VVGN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQ-SISNEKYVDYVVGNMTDVFKEVY 213

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
             GGRKFG +   P    P+S +    +    F+  + +  +HN+ L + L++L  +L G
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTH--CDNL 335
           FKYA  D HT L +R+ +P KYGFK  + ACCGSG  RGI  CG  + GL  ++  C+N+
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRM-GLSQSYELCENV 333

Query: 336 EDYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
            DY+FFD FH TEK  +Q+AELIWSG   I  PYN K LFE
Sbjct: 334 TDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of CmoCh02G006060 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 1.2e-83
Identity = 162/340 (47.65%), Postives = 217/340 (63.82%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYINT + F++N  PYG+T F  PT R +DG        E A
Sbjct: 32  NQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKA 91

Query: 96  GLPLIPPYLDPH--TNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LP IPP L P+   N +  GV+FAS+G+GALAES  G  I L TQ+  FK V KSLR +
Sbjct: 92  WLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSE 151

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG+A  + + S +++LF+ G NDY  PF  +   F  S+++E+FV+ V+GNIT  ++EVY
Sbjct: 152 LGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFK-SNSKEKFVDFVIGNITFVIEEVY 211

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
           K GGRKFGF+ V P    P+S I+   +    F+  + +  +HN+  PD L++L  QL G
Sbjct: 212 KMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSG 271

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNV-EGLPFTHCDNLE 335
           F+YA  D HT L +RI +P KYGFK  + ACCGSG  RGI  CG  +     +  C+N+ 
Sbjct: 272 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 331

Query: 336 DYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
           DY+F+DS H TEK  +Q+AELIW+G   + +PYN K LFE
Sbjct: 332 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363

BLAST of CmoCh02G006060 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 309.7 bits (792), Expect = 4.6e-83
Identity = 156/342 (45.61%), Postives = 223/342 (65.20%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A FLFGDS++DAGNNNYINTT   QANFPPYG+TFF LPT RF+DGR I DF+AEYA
Sbjct: 45  NVTALFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYA 104

Query: 96  GLPLIPPYLDP-HTNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKL 155
            LPLIPP+L+P ++     GVNFAS+G+GAL E+ +GS I L+TQ+  +KKV +  R   
Sbjct: 105 NLPLIPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNF 164

Query: 156 GNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYK 215
           G   ++  IS +++L + G NDY + F  +  +     +  + V++V+GN+T  + E+YK
Sbjct: 165 GKEESKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYK 224

Query: 216 YGGRKFGFMTVPPLGHMPSSRI---KRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGF 275
            GGRKFGF+ VP LG  P+ RI   K       +AS +  +HN+ L + L ++  Q++GF
Sbjct: 225 IGGRKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGF 284

Query: 276 KYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDY 335
           K++  D +  L  R+ +P K+GFK  E ACCG+G +RG+++CG       +  C+N +DY
Sbjct: 285 KFSLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDY 344

Query: 336 VFFDSFHPTEKLFKQLAELIWSG----DAQIVKPYNFKQLFE 370
           +F+DS H T+  + Q A LIW+G    D+ +V PYN   LF+
Sbjct: 345 IFWDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of CmoCh02G006060 vs. ExPASy TrEMBL
Match: A0A6J1G6L0 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451341 PE=3 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 4.4e-222
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS
Sbjct: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP
Sbjct: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300
           QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300

Query: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360
           CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK
Sbjct: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360

Query: 361 PYNFKQLFEYDSMLASY 378
           PYNFKQLFEYDSMLASY
Sbjct: 361 PYNFKQLFEYDSMLASY 377

BLAST of CmoCh02G006060 vs. ExPASy TrEMBL
Match: A0A6J1L1A1 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499513 PE=3 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 8.3e-213
Identity = 361/377 (95.76%), Postives = 368/377 (97.61%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFAL TASPTFPEA+TI LPNCF FFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALQTASPTFPEASTISLPNCFGFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYG+TFFHLPT RFTDGRTIPDFLAEYAGLPLIPPYLDPH +FYDNGVNFASS
Sbjct: 61  DFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESH+GSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNS+FLFNFGGNDYLNP
Sbjct: 121 GSGALAESHKGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300
           QFFEEASSITRLHNQLLPDALQKLGIQL+GFKYAFADTHTLLLQRI+NPIKYGFKV ETA
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLKGFKYAFADTHTLLLQRIINPIKYGFKVTETA 300

Query: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360
           CCGSGAFRGIYNCGRNVEGLPFT CDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIV 
Sbjct: 301 CCGSGAFRGIYNCGRNVEGLPFTECDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVN 360

Query: 361 PYNFKQLFEYDSMLASY 378
           PYNFKQLFEY SMLASY
Sbjct: 361 PYNFKQLFEYGSMLASY 377

BLAST of CmoCh02G006060 vs. ExPASy TrEMBL
Match: A0A6J1G6Q8 (GDSL esterase/lipase 2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451341 PE=3 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.1e-167
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS
Sbjct: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP
Sbjct: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 294
           QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 293

BLAST of CmoCh02G006060 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.6e-163
Identity = 281/377 (74.54%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLP------NCFAFFLFGDSYVDAGNNN 60
           MK PNLG C  FFFILFA     PT  EA  I LP      N F FF+FGDSYVD GNNN
Sbjct: 1   MKFPNLGSC--FFFILFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGNNN 60

Query: 61  YINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNG 120
           YINTT+DFQANFPPYGE+FF + T RFTDGR IPDFL EYA LPL+PPYLDPH + YD G
Sbjct: 61  YINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120

Query: 121 VNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGG 180
           VNFAS G GALA SH+  A+GLQTQMKFFK+V KSLRKKLGNA  Q+  SNS+FLFNFGG
Sbjct: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSS 240
           NDYLNPFDISYDIF T  AQE+FVNMV+GNIT ALKEVYK+GGRKFG M VPPLG+MPSS
Sbjct: 181 NDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSS 240

Query: 241 RIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGF 300
           R+K+S QFFEEASSI RLHN+LLP AL KL  QL+GFKYAFADTHTLLLQRILNP +YGF
Sbjct: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGF 300

Query: 301 KVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSG 360
           KVV+TACCGS  FRG+YNCGR +  LPFTHC NLED++FFDSFHPTEK+FKQL E +WSG
Sbjct: 301 KVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSG 360

Query: 361 DAQIVKPYNFKQLFEYD 372
             ++VKPYNFKQLF+Y+
Sbjct: 361 GLEVVKPYNFKQLFDYE 375

BLAST of CmoCh02G006060 vs. ExPASy TrEMBL
Match: A0A6J1KYX0 (GDSL esterase/lipase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499513 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.3e-161
Identity = 281/293 (95.90%), Postives = 288/293 (98.29%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFAL TASPTFPEA+TI LPNCF FFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALQTASPTFPEASTISLPNCFGFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYG+TFFHLPT RFTDGRTIPDFLAEYAGLPLIPPYLDPH +FYDNGVNFASS
Sbjct: 61  DFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESH+GSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNS+FLFNFGGNDYLNP
Sbjct: 121 GSGALAESHKGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 294
           QFFEEASSITRLHNQLLPDALQKLGIQL+GFKYAFADTHTLLLQRI+NPIKYG
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLKGFKYAFADTHTLLLQRIINPIKYG 293

BLAST of CmoCh02G006060 vs. NCBI nr
Match: XP_022947487.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 780.0 bits (2013), Expect = 9.1e-222
Identity = 377/377 (100.00%), Postives = 377/377 (100.00%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS
Sbjct: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP
Sbjct: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300
           QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300

Query: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360
           CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK
Sbjct: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360

Query: 361 PYNFKQLFEYDSMLASY 378
           PYNFKQLFEYDSMLASY
Sbjct: 361 PYNFKQLFEYDSMLASY 377

BLAST of CmoCh02G006060 vs. NCBI nr
Match: XP_023532785.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753.8 bits (1945), Expect = 7.0e-214
Identity = 364/379 (96.04%), Postives = 370/379 (97.63%), Query Frame = 0

Query: 1   MKLPNLGFC--FFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINT 60
           MKLPNLGFC  FFFFFILFA  TASPTFPEA+TIPLPNCFAFFLFGDSYVDAGNNNYINT
Sbjct: 1   MKLPNLGFCCFFFFFFILFAFQTASPTFPEASTIPLPNCFAFFLFGDSYVDAGNNNYINT 60

Query: 61  TADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFA 120
           TADFQANFPPYGETFFHLPT RFTDGRTIPDFLAEYAGLPLIPPYLDPH +FYDNGVNFA
Sbjct: 61  TADFQANFPPYGETFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDNGVNFA 120

Query: 121 SSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYL 180
           SSGSGALAESHEGSAIGLQTQMKFFK VMKSLRKKLGNASAQTLISNS+FLFNFGGNDYL
Sbjct: 121 SSGSGALAESHEGSAIGLQTQMKFFKNVMKSLRKKLGNASAQTLISNSVFLFNFGGNDYL 180

Query: 181 NPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKR 240
           NPFDISYDIFNTSSAQE+FVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKR
Sbjct: 181 NPFDISYDIFNTSSAQEQFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKR 240

Query: 241 SVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVE 300
           SVQFFEEASSITRLHNQ+LPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKV E
Sbjct: 241 SVQFFEEASSITRLHNQVLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVAE 300

Query: 301 TACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQI 360
           TACCGSGAFRG YNCGRNVEGLPFTHCDNLEDY+FFDSFHPTEKLFKQLAELIWSGDAQI
Sbjct: 301 TACCGSGAFRGFYNCGRNVEGLPFTHCDNLEDYIFFDSFHPTEKLFKQLAELIWSGDAQI 360

Query: 361 VKPYNFKQLFEYDSMLASY 378
           VKPYNFKQLFEYDSMLASY
Sbjct: 361 VKPYNFKQLFEYDSMLASY 379

BLAST of CmoCh02G006060 vs. NCBI nr
Match: XP_023006840.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 749.2 bits (1933), Expect = 1.7e-212
Identity = 361/377 (95.76%), Postives = 368/377 (97.61%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFAL TASPTFPEA+TI LPNCF FFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALQTASPTFPEASTISLPNCFGFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYG+TFFHLPT RFTDGRTIPDFLAEYAGLPLIPPYLDPH +FYDNGVNFASS
Sbjct: 61  DFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESH+GSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNS+FLFNFGGNDYLNP
Sbjct: 121 GSGALAESHKGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETA 300
           QFFEEASSITRLHNQLLPDALQKLGIQL+GFKYAFADTHTLLLQRI+NPIKYGFKV ETA
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLKGFKYAFADTHTLLLQRIINPIKYGFKVTETA 300

Query: 301 CCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVK 360
           CCGSGAFRGIYNCGRNVEGLPFT CDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIV 
Sbjct: 301 CCGSGAFRGIYNCGRNVEGLPFTECDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVN 360

Query: 361 PYNFKQLFEYDSMLASY 378
           PYNFKQLFEY SMLASY
Sbjct: 361 PYNFKQLFEYGSMLASY 377

BLAST of CmoCh02G006060 vs. NCBI nr
Match: XP_022947488.1 (GDSL esterase/lipase 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 599.4 bits (1544), Expect = 2.2e-167
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60
           MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA
Sbjct: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLPNCFAFFLFGDSYVDAGNNNYINTTA 60

Query: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120
           DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS
Sbjct: 61  DFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNFASS 120

Query: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180
           GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP
Sbjct: 121 GSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNP 180

Query: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240
           FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV
Sbjct: 181 FDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSV 240

Query: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 294
           QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG
Sbjct: 241 QFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 293

BLAST of CmoCh02G006060 vs. NCBI nr
Match: XP_022928027.1 (GDSL esterase/lipase 3-like [Cucurbita moschata])

HSP 1 Score: 584.3 bits (1505), Expect = 7.3e-163
Identity = 281/377 (74.54%), Postives = 312/377 (82.76%), Query Frame = 0

Query: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLP------NCFAFFLFGDSYVDAGNNN 60
           MK PNLG C  FFFILFA     PT  EA  I LP      N F FF+FGDSYVD GNNN
Sbjct: 1   MKFPNLGSC--FFFILFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGNNN 60

Query: 61  YINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNG 120
           YINTT+DFQANFPPYGE+FF + T RFTDGR IPDFL EYA LPL+PPYLDPH + YD G
Sbjct: 61  YINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120

Query: 121 VNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGG 180
           VNFAS G GALA SH+  A+GLQTQMKFFK+V KSLRKKLGNA  Q+  SNS+FLFNFGG
Sbjct: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSS 240
           NDYLNPFDISYDIF T  AQE+FVNMV+GNIT ALKEVYK+GGRKFG M VPPLG+MPSS
Sbjct: 181 NDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSS 240

Query: 241 RIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGF 300
           R+K+S QFFEEASSI RLHN+LLP AL KL  QL+GFKYAFADTHTLLLQRILNP +YGF
Sbjct: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGF 300

Query: 301 KVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSG 360
           KVV+TACCGS  FRG+YNCGR +  LPFTHC NLED++FFDSFHPTEK+FKQL E +WSG
Sbjct: 301 KVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSG 360

Query: 361 DAQIVKPYNFKQLFEYD 372
             ++VKPYNFKQLF+Y+
Sbjct: 361 GLEVVKPYNFKQLFDYE 375

BLAST of CmoCh02G006060 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 315.1 bits (806), Expect = 7.7e-86
Identity = 165/341 (48.39%), Postives = 220/341 (64.52%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYI+T +  ++N+ PYG+T F  PT R +DGR IPDF+AEYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 96  GLPLIPPYLDPH--TNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LPLIPP L P    + +  GVNFAS G+GAL  +  G  I L+TQ+  FKKV + LR K
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG+A  + +IS +++LF+ G NDY  PF  +  +F  S + E++V+ VVGN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQ-SISNEKYVDYVVGNMTDVFKEVY 213

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
             GGRKFG +   P    P+S +    +    F+  + +  +HN+ L + L++L  +L G
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTH--CDNL 335
           FKYA  D HT L +R+ +P KYGFK  + ACCGSG  RGI  CG  + GL  ++  C+N+
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRM-GLSQSYELCENV 333

Query: 336 EDYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
            DY+FFD FH TEK  +Q+AELIWSG   I  PYN K LFE
Sbjct: 334 TDYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of CmoCh02G006060 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 311.6 bits (797), Expect = 8.6e-85
Identity = 162/340 (47.65%), Postives = 217/340 (63.82%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYINT + F++N  PYG+T F  PT R +DG        E A
Sbjct: 32  NQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKA 91

Query: 96  GLPLIPPYLDPH--TNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LP IPP L P+   N +  GV+FAS+G+GALAES  G  I L TQ+  FK V KSLR +
Sbjct: 92  WLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSE 151

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG+A  + + S +++LF+ G NDY  PF  +   F  S+++E+FV+ V+GNIT  ++EVY
Sbjct: 152 LGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFK-SNSKEKFVDFVIGNITFVIEEVY 211

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
           K GGRKFGF+ V P    P+S I+   +    F+  + +  +HN+  PD L++L  QL G
Sbjct: 212 KMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSG 271

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNV-EGLPFTHCDNLE 335
           F+YA  D HT L +RI +P KYGFK  + ACCGSG  RGI  CG  +     +  C+N+ 
Sbjct: 272 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 331

Query: 336 DYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLFE 370
           DY+F+DS H TEK  +Q+AELIW+G   + +PYN K LFE
Sbjct: 332 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFE 363

BLAST of CmoCh02G006060 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 309.7 bits (792), Expect = 3.3e-84
Identity = 156/342 (45.61%), Postives = 223/342 (65.20%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A FLFGDS++DAGNNNYINTT   QANFPPYG+TFF LPT RF+DGR I DF+AEYA
Sbjct: 45  NVTALFLFGDSFLDAGNNNYINTTTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYA 104

Query: 96  GLPLIPPYLDP-HTNFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKL 155
            LPLIPP+L+P ++     GVNFAS+G+GAL E+ +GS I L+TQ+  +KKV +  R   
Sbjct: 105 NLPLIPPFLEPGNSQKKLYGVNFASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNF 164

Query: 156 GNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYK 215
           G   ++  IS +++L + G NDY + F  +  +     +  + V++V+GN+T  + E+YK
Sbjct: 165 GKEESKKRISRAVYLISIGSNDYSSIFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYK 224

Query: 216 YGGRKFGFMTVPPLGHMPSSRI---KRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGF 275
            GGRKFGF+ VP LG  P+ RI   K       +AS +  +HN+ L + L ++  Q++GF
Sbjct: 225 IGGRKFGFLNVPDLGCFPALRILQPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGF 284

Query: 276 KYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDY 335
           K++  D +  L  R+ +P K+GFK  E ACCG+G +RG+++CG       +  C+N +DY
Sbjct: 285 KFSLFDMNKSLRLRMQHPSKFGFKEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDY 344

Query: 336 VFFDSFHPTEKLFKQLAELIWSG----DAQIVKPYNFKQLFE 370
           +F+DS H T+  + Q A LIW+G    D+ +V PYN   LF+
Sbjct: 345 IFWDSLHLTQNTYNQFANLIWNGGHMSDSLVVGPYNINNLFQ 383

BLAST of CmoCh02G006060 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 309.3 bits (791), Expect = 4.2e-84
Identity = 164/346 (47.40%), Postives = 216/346 (62.43%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F FGDS  +AGNNNY ++ + F++NF PYG+T F  PT R +DGR + DF+AEYA
Sbjct: 34  NQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYA 93

Query: 96  GLPLIPPYLDPHTNFYDN-----GVNFASSGSGALAESHEGSAIGLQ----TQMKFFKKV 155
            LPLIPP L P    Y N     G+NFA++ +G  A +  GS   L     TQ+  FK V
Sbjct: 94  WLPLIPPNLQPG---YSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNV 153

Query: 156 MKSLRKKLGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNIT 215
            K+LR  LG+A A+ +IS +++LF+ G NDY  PF  +   F +++ +ERF++ V+GN T
Sbjct: 154 EKTLRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTF-SNTTKERFIDFVIGNTT 213

Query: 216 IALKEVYKYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQK 275
             ++E+YK G RKFGF+++ P G  PS+ I  S +    FE  + +  LHNQ  P  L++
Sbjct: 214 TVIEELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTELINLHNQEFPKVLRR 273

Query: 276 LGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNCG-RNVEGLPF 335
           L  +L GFKYA  D HT L QRI NP +YGFK  E ACCGSG  RGI  CG RN     +
Sbjct: 274 LERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGPSQGY 333

Query: 336 THCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQIVKPYNFKQLF 369
             C+N +DYVFFD  H TE   +Q+AELIWSG   +  PYN K LF
Sbjct: 334 KLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375

BLAST of CmoCh02G006060 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 305.8 bits (782), Expect = 4.7e-83
Identity = 162/325 (49.85%), Postives = 208/325 (64.00%), Query Frame = 0

Query: 36  NCFAFFLFGDSYVDAGNNNYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYA 95
           N  A F+FGDS  DAGNNNYI+T   F++N+ PYG+T F  PT R +DGRTIPDF+AEYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 96  GLPLIPPYLDPHT--NFYDNGVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKK 155
            LPLIP YL P    N +  GV+FAS+G+GAL  +  G  I L++Q+  FKKV K LR  
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 156 LGNASAQTLISNSIFLFNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVY 215
           LG A  + +IS +++LF+ G NDY  PF  +  IF  SS QE +V+ VVGN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQ-SSPQEIYVDFVVGNTTAVIKEVY 215

Query: 216 KYGGRKFGFMTVPPLGHMPSSRIKRSVQF---FEEASSITRLHNQLLPDALQKLGIQLEG 275
           K GGRKFGF+ +      P+S I    +    F+  + +  LHN+ L   L++L  +L G
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 276 FKYAFADTHTLLLQRILNPIKYGFKVVETACCGSGAFRGIYNC-GRNVEGLPFTHCDNLE 335
           FKYA  D HT L  R+ NP KYGFK  + ACCG+G  RGI  C GR      +  C+ + 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 336 DYVFFDSFHPTEKLFKQLAELIWSG 355
           DY+FFD FH TEK  +Q+AELIWSG
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSG 359