CmoCh02G005440 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G005440
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	E3 ubiquitin-protein ligase RNF126-like
Location	Cmo_Chr02: 3092971 .. 3093642 (+)
RNA-Seq Expression	CmoCh02G005440
Synteny	CmoCh02G005440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTAACCTTCCACGACTATCTGTCCTTCCAATGTTCCATCCAAAGCACTTGCAACGGCACCGTGGCTTCCGACCAGCAACCCATGCAGCTCCTGGAACTCCGACTCGGAAATTCGACCCATCTGATTTCTCGATCTTCTCGCCAAATTCTCCAACAAACTCCCACAACCCCAATGGGCGATGCTCTGTTTCCTTTTGTTCTGCGCGACCTCCAACACCCACCCTTCCTCTCCAACTGCTATGTTCTTCAGTTTTTGTCTTCCTTCAGCATCCCCATCGCCGCCTGCGATATCATCTATGAAGAGATCTCCTCGTTTGCGCTCCAATTGGCCGCCGGCAACGTGGATCTCAACTTCCACATCGTCGTCGAAGTGGATGTCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGGCTGAGATGAATGGGGCGCCGGCGTCGGCGATTGAGAGGTTGCAGAGGCAGGAATTTGATGGGTTAAGGGAGGAAGAGGAGGAGGGGGATTGCAGTGTTTGTTGCGAAGCGCTGAAGGGAGAAGAGGAGGTGAGTAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATTCTCAAGTGGCTTCAGATGAGCAATTCGTGTCCTCTGTGTAGAACCAAATTAGAACAATAA

mRNA sequence

ATGTCTTTAACCTTCCACGACTATCTGTCCTTCCAATGTTCCATCCAAAGCACTTGCAACGGCACCGTGGCTTCCGACCAGCAACCCATGCAGCTCCTGGAACTCCGACTCGGAAATTCGACCCATCTGATTTCTCGATCTTCTCGCCAAATTCTCCAACAAACTCCCACAACCCCAATGGGCGATGCTCTGTTTCCTTTTGTTCTGCGCGACCTCCAACACCCACCCTTCCTCTCCAACTGCTATGTTCTTCAGTTTTTGTCTTCCTTCAGCATCCCCATCGCCGCCTGCGATATCATCTATGAAGAGATCTCCTCGTTTGCGCTCCAATTGGCCGCCGGCAACGTGGATCTCAACTTCCACATCGTCGTCGAAGTGGATGTCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGGCTGAGATGAATGGGGCGCCGGCGTCGGCGATTGAGAGGTTGCAGAGGCAGGAATTTGATGGGTTAAGGGAGGAAGAGGAGGAGGGGGATTGCAGTGTTTGTTGCGAAGCGCTGAAGGGAGAAGAGGAGGTGAGTAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATTCTCAAGTGGCTTCAGATGAGCAATTCGTGTCCTCTGTGTAGAACCAAATTAGAACAATAA

Coding sequence (CDS)

ATGTCTTTAACCTTCCACGACTATCTGTCCTTCCAATGTTCCATCCAAAGCACTTGCAACGGCACCGTGGCTTCCGACCAGCAACCCATGCAGCTCCTGGAACTCCGACTCGGAAATTCGACCCATCTGATTTCTCGATCTTCTCGCCAAATTCTCCAACAAACTCCCACAACCCCAATGGGCGATGCTCTGTTTCCTTTTGTTCTGCGCGACCTCCAACACCCACCCTTCCTCTCCAACTGCTATGTTCTTCAGTTTTTGTCTTCCTTCAGCATCCCCATCGCCGCCTGCGATATCATCTATGAAGAGATCTCCTCGTTTGCGCTCCAATTGGCCGCCGGCAACGTGGATCTCAACTTCCACATCGTCGTCGAAGTGGATGTCATCGAAATGATATGGGTGGACCTGGACCCATTCCTTGTTGAAGAATATTCTCCGGCTGAGATGAATGGGGCGCCGGCGTCGGCGATTGAGAGGTTGCAGAGGCAGGAATTTGATGGGTTAAGGGAGGAAGAGGAGGAGGGGGATTGCAGTGTTTGTTGCGAAGCGCTGAAGGGAGAAGAGGAGGTGAGTAGAATCCCGTGTGGTCATGTGTATCACAAATGCTGTATTCTCAAGTGGCTTCAGATGAGCAATTCGTGTCCTCTGTGTAGAACCAAATTAGAACAATAA

Protein sequence

MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPMGDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ

Homology

[Close]

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot
Match: E9QAU8 (E3 ubiquitin-protein ligase RNF165 OS=Mus musculus OX=10090 GN=Rnf165 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.2e-09
Identity = 31/85 (36.47%), Postives = 48/85 (56.47%), Query Frame = 0


Query: 151 GAPASAIERL---------QRQEFDGLREEEEEGD----CSVCCEALKGEEEVSRIPCGH 210
           GA  + IER          + Q+  G ++E EE D    C++C   L+  E+V R+PC H
Sbjct: 256 GAVQNTIERFTFPHKYKKRRPQDSKGKKDEGEESDTDEKCTICLSMLEDGEDVRRLPCMH 315

Query: 211 VYHKCCILKWLQMSNSCPLCRTKLE 223
           ++H+ C+ +WL MS  CP+CR  +E
Sbjct: 316 LFHQLCVDQWLAMSKKCPICRVDIE 340

[Close]

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot
Match: Q6ZSG1 (E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.5e-09
Identity = 31/85 (36.47%), Postives = 48/85 (56.47%), Query Frame = 0


Query: 151 GAPASAIERL---------QRQEFDGLREEEEEGD----CSVCCEALKGEEEVSRIPCGH 210
           GA  + IER          + Q+  G ++E EE D    C++C   L+  E+V R+PC H
Sbjct: 255 GAVQNTIERFTFPHKYKKRRPQDGKGKKDEGEESDTDEKCTICLSMLEDGEDVRRLPCMH 314

Query: 211 VYHKCCILKWLQMSNSCPLCRTKLE 223
           ++H+ C+ +WL MS  CP+CR  +E
Sbjct: 315 LFHQLCVDQWLAMSKKCPICRVDIE 339

[Close]

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot
Match: Q9VHI7 (E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.4e-09
Identity = 28/88 (31.82%), Postives = 49/88 (55.68%), Query Frame = 0


Query: 136 LDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEE--EEGDCSVCCEALKGEEEVSRI 195
           LD  + +  +  E +G P  + +R+       +  EE   +  CS+C +  K +E V ++
Sbjct: 210 LDTIVTQMLNQMETSGPPPLSAQRINEIPNVQINAEEVNRKIQCSICWDDFKIDETVRKL 269

Query: 196 PCGHVYHKCCILKWLQMSNSCPLCRTKL 222
           PC H+YH+ CI+ WL + ++CP+CR  L
Sbjct: 270 PCSHLYHENCIVPWLNLHSTCPICRKSL 297

[Close]

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.5e-09
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 0


Query: 136 LDPFLVEEYSPAEMNGAPAS--AIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRI 195
           ++P L       E    PAS  +I+ +   E DG      EG+C +C E  K EE V  +
Sbjct: 75  MNPLLRSLLESREEGRPPASKASIDAMPIVEIDGC-----EGECVICLEEWKSEETVKEM 134

Query: 196 PCGHVYHKCCILKWLQMSNSCPLCRTKL 222
           PC H +H  CI KWL    SCP+CR ++
Sbjct: 135 PCKHRFHGGCIEKWLGFHGSCPVCRYEM 157

[Close]

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.5e-09
Identity = 29/81 (35.80%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 142 EEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHK 201
           E Y     N  P    E L R       +++ + +CSVC    +G+ E++++ CGH++HK
Sbjct: 74  ESYLEEFRNRTPTLRFESLCR------CKKQADNECSVCLSKFQGDSEINKLKCGHLFHK 133

Query: 202 CCILKWLQMSN-SCPLCRTKL 222
            C+ KW+   N +CPLCRT L
Sbjct: 134 TCLEKWIDYWNITCPLCRTPL 148

[Close]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 4.3e-124
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC
Sbjct: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL
Match: A0A6J1KYV1 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499496 PE=4 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 2.1e-118
Identity = 214/223 (95.96%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTFHDYLSFQCSIQSTCNGTVAS QQP+QLLELRLG+STHLISRSSRQILQQTP+TP+
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASGQQPIQLLELRLGHSTHLISRSSRQILQQTPSTPI 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGN+DLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVVEVDV EMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQ+FDGLREEEEEGDCSVC
Sbjct: 121 HIVVEVDVNEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHK CILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKFCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL
Match: A0A6J1G809 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111451600 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 9.3e-103
Identity = 188/223 (84.30%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTF D LSFQCS ++   GT+AS +QPMQ +ELRLG+S+HLISRSSRQILQQTP+TPM
Sbjct: 1   MSLTFGDSLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPF+LRDL HPPFLS  +VL+FLSSFSIPIA C+II+ EISSFAL LAAGNVDLNF
Sbjct: 61  GDALFPFILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVV+VD IEMIWVDLDPFL+EEYSP  MNGAPASAIERLQRQEFDGLR EEEEG+CSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLIEEYSPPAMNGAPASAIERLQRQEFDGLR-EEEEGECSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCIL+WLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLEQ 222

[Close]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL
Match: A0A6J1L387 (RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.7e-102
Identity = 184/223 (82.51%), Postives = 206/223 (92.38%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTF D LSFQ SI++   GT+AS + PMQ +ELRLG+S+HLISRSSRQILQQ+P+TP+
Sbjct: 1   MSLTFGDSLSFQFSIRTNTTGTLASHEHPMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           G++LFPF+LRDL+HPPFL N +VL FLSSFSIP+AACDIIY+EISSFAL+L A NVDLNF
Sbjct: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPVAACDIIYQEISSFALRLTADNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVV+VD IEMIWVDLDPFLVEEYSP  MNGAPASAIERLQRQ+FDGLREEEEEGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPAMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEAL+GEEEVSRIPCGHVYHKCCILKWLQ+SNSCPLCRTKLEQ
Sbjct: 181 CEALRGEEEVSRIPCGHVYHKCCILKWLQISNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL
Match: A0A6J1G826 (E3 ubiquitin-protein ligase RNF38-like OS=Cucurbita moschata OX=3662 GN=LOC111451599 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 5.1e-93
Identity = 167/223 (74.89%), Postives = 197/223 (88.34%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSL+ HDYLS+QCS++ TCN ++ SD+QPMQL+ELR+G+S +LISRSS QILQ++P+TPM
Sbjct: 48  MSLSSHDYLSYQCSMRGTCNDSLGSDEQPMQLMELRVGHSRNLISRSSHQILQRSPSTPM 107

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALF F+LRD + PPFL N  VL+FLSSF IPI AC++IY++ISSFA  + A +V LNF
Sbjct: 108 GDALFSFILRDQEQPPFLENSSVLRFLSSFRIPIPACNVIYDQISSFAQHMTADDVHLNF 167

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           H+VV+V+V EM WVDLDPFLVEE SP  MNGAPASAIERLQRQEF GLREEEEEGDCSVC
Sbjct: 168 HMVVDVNVSEMTWVDLDPFLVEENSPPPMNGAPASAIERLQRQEFGGLREEEEEGDCSVC 227

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEE SRIPCGHVYHKCCILKWLQ+SNSCPLCRT+L++
Sbjct: 228 CEALKGEEEGSRIPCGHVYHKCCILKWLQISNSCPLCRTQLQE 270

[Close]

BLAST of CmoCh02G005440 vs. NCBI nr
Match: XP_022947933.1 (E3 ubiquitin-protein ligase RNF126-like [Cucurbita moschata] >XP_023532920.1 E3 ubiquitin-protein ligase RNF126-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.8 bits (1166), Expect = 8.9e-124
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC
Sbjct: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. NCBI nr
Match: KAG6605221.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 452.6 bits (1163), Expect = 2.0e-123
Identity = 222/223 (99.55%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLR+EEEEGDCSVC
Sbjct: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLRDEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. NCBI nr
Match: XP_023006826.1 (E3 ubiquitin-protein ligase RNF126-like [Cucurbita maxima])

HSP 1 Score: 434.9 bits (1117), Expect = 4.3e-118
Identity = 214/223 (95.96%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTFHDYLSFQCSIQSTCNGTVAS QQP+QLLELRLG+STHLISRSSRQILQQTP+TP+
Sbjct: 1   MSLTFHDYLSFQCSIQSTCNGTVASGQQPIQLLELRLGHSTHLISRSSRQILQQTPSTPI 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGN+DLNF
Sbjct: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVVEVDV EMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQ+FDGLREEEEEGDCSVC
Sbjct: 121 HIVVEVDVNEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHK CILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKFCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. NCBI nr
Match: XP_022947840.1 (probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata])

HSP 1 Score: 382.9 bits (982), Expect = 1.9e-102
Identity = 188/223 (84.30%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTF D LSFQCS ++   GT+AS +QPMQ +ELRLG+S+HLISRSSRQILQQTP+TPM
Sbjct: 1   MSLTFGDSLSFQCSFRTNSTGTLASHEQPMQFVELRLGHSSHLISRSSRQILQQTPSTPM 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           GDALFPF+LRDL HPPFLS  +VL+FLSSFSIPIA C+II+ EISSFAL LAAGNVDLNF
Sbjct: 61  GDALFPFILRDLLHPPFLSKSHVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVV+VD IEMIWVDLDPFL+EEYSP  MNGAPASAIERLQRQEFDGLR EEEEG+CSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLIEEYSPPAMNGAPASAIERLQRQEFDGLR-EEEEGECSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCIL+WLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILRWLQMSNSCPLCRTKLEQ 222

[Close]

BLAST of CmoCh02G005440 vs. NCBI nr
Match: KAG6605228.1 (E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 382.9 bits (982), Expect = 1.9e-102
Identity = 188/223 (84.30%), Postives = 206/223 (92.38%), Query Frame = 0


Query: 1   MSLTFHDYLSFQCSIQSTCNGTVASDQQPMQLLELRLGNSTHLISRSSRQILQQTPTTPM 60
           MSLTF D LSFQ SI++    TVAS +Q MQ +ELRLG+S+HLISRSSRQILQQ+P+TP+
Sbjct: 1   MSLTFGDSLSFQFSIRTNTTATVASHEQLMQFVELRLGHSSHLISRSSRQILQQSPSTPL 60

Query: 61  GDALFPFVLRDLQHPPFLSNCYVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNF 120
           G++LFPF+LRDL+HPPFL N +VL FLSSFSIPIAACDIIY+EISSFAL+LAA NVDLNF
Sbjct: 61  GESLFPFILRDLEHPPFLPNYHVLHFLSSFSIPIAACDIIYQEISSFALRLAADNVDLNF 120

Query: 121 HIVVEVDVIEMIWVDLDPFLVEEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVC 180
           HIVV+VD IEMIWVDLDPFLVEEYSP  MNGAPASAIERLQRQ+FDGLREEEEEGDCSVC
Sbjct: 121 HIVVDVDAIEMIWVDLDPFLVEEYSPPPMNGAPASAIERLQRQKFDGLREEEEEGDCSVC 180

Query: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 224
           CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ
Sbjct: 181 CEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLCRTKLEQ 223

[Close]

BLAST of CmoCh02G005440 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.8e-10
Identity = 28/66 (42.42%), Postives = 44/66 (66.67%), Query Frame = 0


Query: 159 RLQRQEFDGLREEEEEGDCSVCCEALKG---EEEVSRIPCGHVYHKCCILKWLQMSNSCP 218
           R++ +EF  L+ E E   CS+C E+L       +V+R+ C HV+H  C+L+WL+  N+CP
Sbjct: 141 RMKAEEFKSLKMETE--PCSICLESLVSGPKPRDVTRMTCSHVFHNGCLLEWLKRKNTCP 200

Query: 219 LCRTKL 222
           LCRT++
Sbjct: 201 LCRTEI 204

[Close]

BLAST of CmoCh02G005440 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.4e-10
Identity = 27/62 (43.55%), Postives = 39/62 (62.90%), Query Frame = 0


Query: 159 RLQRQEF-DGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHKCCILKWLQMSNSCPLC 218
           RL++Q++    R  +E   C VC E  K EEE+ R+ CGH +H  CI +WL+  N CP+C
Sbjct: 573 RLKQQKYKSSTRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQCIKEWLKQKNLCPIC 632

Query: 219 RT 220
           +T
Sbjct: 633 KT 634

[Close]

BLAST of CmoCh02G005440 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.4e-10
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 0


Query: 136 LDPFLVEEYSPAEMNGAPAS--AIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRI 195
           ++P L       E    PAS  +I+ +   E DG      EG+C +C E  K EE V  +
Sbjct: 75  MNPLLRSLLESREEGRPPASKASIDAMPIVEIDGC-----EGECVICLEEWKSEETVKEM 134

Query: 196 PCGHVYHKCCILKWLQMSNSCPLCRTKL 222
           PC H +H  CI KWL    SCP+CR ++
Sbjct: 135 PCKHRFHGGCIEKWLGFHGSCPVCRYEM 157

[Close]

BLAST of CmoCh02G005440 vs. TAIR 10
Match: AT2G04240.1 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.4e-10
Identity = 29/81 (35.80%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 142 EEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHK 201
           E Y     N  P    E L R       +++ + +CSVC    +G+ E++++ CGH++HK
Sbjct: 74  ESYLEEFRNRTPTLRFESLCR------CKKQADNECSVCLSKFQGDSEINKLKCGHLFHK 133

Query: 202 CCILKWLQMSN-SCPLCRTKL 222
            C+ KW+   N +CPLCRT L
Sbjct: 134 TCLEKWIDYWNITCPLCRTPL 148

[Close]

BLAST of CmoCh02G005440 vs. TAIR 10
Match: AT2G04240.2 (RING/U-box superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.4e-10
Identity = 29/81 (35.80%), Postives = 45/81 (55.56%), Query Frame = 0


Query: 142 EEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHK 201
           E Y     N  P    E L R       +++ + +CSVC    +G+ E++++ CGH++HK
Sbjct: 74  ESYLEEFRNRTPTLRFESLCR------CKKQADNECSVCLSKFQGDSEINKLKCGHLFHK 133

Query: 202 CCILKWLQMSN-SCPLCRTKL 222
            C+ KW+   N +CPLCRT L
Sbjct: 134 TCLEKWIDYWNITCPLCRTPL 148

The following BLAST results are available for this feature:

BLAST of CmoCh02G005440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
E9QAU8	1.2e-09	36.47	E3 ubiquitin-protein ligase RNF165 OS=Mus musculus OX=10090 GN=Rnf165 PE=1 SV=1	[more]
Q6ZSG1	1.5e-09	36.47	E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1	[more]
Q9VHI7	4.4e-09	31.82	E3 ubiquitin-protein ligase Iruka OS=Drosophila melanogaster OX=7227 GN=Iru PE=1...	[more]
Q9LQX2	7.5e-09	37.50	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q9SI09	7.5e-09	35.80	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]

BLAST of CmoCh02G005440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7Z5	4.3e-124	100.00	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KYV1	2.1e-118	95.96	E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499...	[more]
A0A6J1G809	9.3e-103	84.30	probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1L387	2.7e-102	82.51	RING-H2 finger protein ATL16-like OS=Cucurbita maxima OX=3661 GN=LOC111499493 PE...	[more]
A0A6J1G826	5.1e-93	74.89	E3 ubiquitin-protein ligase RNF38-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]

BLAST of CmoCh02G005440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947933.1	8.9e-124	100.00	E3 ubiquitin-protein ligase RNF126-like [Cucurbita moschata] >XP_023532920.1 E3 ...	[more]
KAG6605221.1	2.0e-123	99.55	E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023006826.1	4.3e-118	95.96	E3 ubiquitin-protein ligase RNF126-like [Cucurbita maxima]	[more]
XP_022947840.1	1.9e-102	84.30	probable E3 ubiquitin-protein ligase RHA4A [Cucurbita moschata]	[more]
KAG6605228.1	1.9e-102	84.30	E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmoCh02G005440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05350.1	1.8e-10	42.42	RING/U-box superfamily protein	[more]
AT1G45180.1	2.4e-10	43.55	RING/U-box superfamily protein	[more]
AT1G26800.1	5.4e-10	37.50	RING/U-box superfamily protein	[more]
AT2G04240.1	5.4e-10	35.80	RING/U-box superfamily protein	[more]
AT2G04240.2	5.4e-10	35.80	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..217 e-value: 6.1E-6 score: 35.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..218 e-value: 8.8E-12 score: 45.2
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 177..218 score: 12.902362
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 141..223 e-value: 7.2E-20 score: 72.5
None	No IPR available	PANTHER	PTHR45931:SF9	E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKE	coord: 79..222
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 79..222
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 169..221

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G005440.1	CmoCh02G005440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity