CmoCh02G005080 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh02G005080 vs. ExPASy Swiss-Prot
Match: Q9M3H5 (Probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HMA1 PE=2 SV=2)

HSP 1 Score: 1066.6 bits (2757), Expect = 1.4e-310
Identity = 553/767 (72.10%), Postives = 645/767 (84.09%), Query Frame = 0

Query: 57  AANDHSNHDEHHRVHNHHHGHHHHHGHHHC---DHDAELTGPQRALIGFAKAIRWTDLAN 116
           A  DH +HD HH     HH HHHHH  H C   +  AE + PQ+ L GFAKAI W  LAN
Sbjct: 63  AVEDH-HHDHHHDDEQDHHNHHHHHHQHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLAN 122

Query: 117 YLREHLQLCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISG 176
           YLREHL LCC + A+F+AAAVCPYL P+P++K  QNA + + FPLVGVS+SLDAL DI+G
Sbjct: 123 YLREHLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAG 182

Query: 177 GKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDS 236
           GKVNIHVLMALAAFAS+FMGNALEGG+LL MFNL+HIAEE+F SR+M+DVKELKES PDS
Sbjct: 183 GKVNIHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDS 242

Query: 237 ALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLT 296
           AL+++ + G +PNI+DLSY+ VPVH V+V SY+LVG GE VPVDCEV+QGSAT+TIEHLT
Sbjct: 243 ALLIEVHNGNVPNISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLT 302

Query: 297 GEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 356
           GE+KPLE   GDRVPGGARNLDGR+IVKATK W +STL++IV LTEEA  NKP+LQRWLD
Sbjct: 303 GEVKPLEAKAGDRVPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLD 362

Query: 357 EFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCA 416
           EFGE+YSK VVVLS+A+A +GP LFKWPF+ T+       ACRGSVYRALGLMVAASPCA
Sbjct: 363 EFGENYSKVVVVLSLAIAFLGPFLFKWPFLSTA-------ACRGSVYRALGLMVAASPCA 422

Query: 417 LAVAPLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYG 476
           LAVAPLAYA AISSCARKGILLKG  VLDAL+SCHTIAFDKTGTLTTG L  KAIEPIYG
Sbjct: 423 LAVAPLAYATAISSCARKGILLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYG 482

Query: 477 HQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEY 536
           HQ   N S   +CCIP CE+EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPS  V++ EY
Sbjct: 483 HQGGTNSSVI-TCCIPNCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEY 542

Query: 537 FPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFV 596
           FPGRGL A + G+++  E  +L KASLGS++FITSL+KS+ +SK+IK+A + SSYG +FV
Sbjct: 543 FPGRGLTATVNGVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFV 602

Query: 597 HAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKE 656
           HAAL+VD KVTLIHLED+P PGVS +IAEL   A+L VMMLTGDH+SSA RVA AVGI E
Sbjct: 603 HAALSVDQKVTLIHLEDQPRPGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITE 662

Query: 657 VYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 716
           VY +LKPEDKL+HVK+I+RE GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD
Sbjct: 663 VYCNLKPEDKLNHVKNIAREAGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 722

Query: 717 VLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGG 776
           +LLLRDNI+GVPFC++KSRQTT+LVKQN +LAL SIF+A+LP+VLGF+PLWLTVLLHEGG
Sbjct: 723 ILLLRDNITGVPFCVAKSRQTTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGG 782

Query: 777 TLLVCLNSIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVH 821
           TLLVCLNS+R LN+PSWSW+QD+  L+ KL+ +    S +   ++ H
Sbjct: 783 TLLVCLNSVRGLNDPSWSWKQDIVHLINKLRSQEPTSSSSNSLSSAH 819

BLAST of CmoCh02G005080 vs. ExPASy Swiss-Prot
Match: P58414 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) OX=169963 GN=cadA PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 4.5e-75
Identity = 183/638 (28.68%), Postives = 325/638 (50.94%), Query Frame = 0

Query: 151 LIAIAFPLVGVSSSLDALTDISGGKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHI 210
           L  IA  + G +   +   ++      +  LM +A   +  +G   EG I++ +F  S +
Sbjct: 118 LYVIAIVVGGFNLFKEGFANLIKLDFTMESLMTIAIIGASIIGEWAEGSIVVILFAFSEV 177

Query: 211 AEEYFISRAMIDVKELKESYPDSALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGA 270
            E Y + +A   ++ L +  P  AL+            D   Q + V D+Q+   +++  
Sbjct: 178 LERYSMDKARQSIRSLMDIAPKEALIR----------RDDVEQMIAVSDIQIGDIMIIKP 237

Query: 271 GESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKEST 330
           G+ + +D  V +G + +    +TGE  P+E  V D V  G  N +G + VK TK  +++T
Sbjct: 238 GQKIAMDGVVIKGYSAINQSAITGESIPVEKKVDDEVFAGTLNEEGLLEVKVTKHVEDTT 297

Query: 331 LSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVT 390
           +S+I++L EEAQ  +   Q ++D+F ++Y+  ++++++ V +V P+ F   +        
Sbjct: 298 ISKIIHLVEEAQGERAPAQAFVDKFAKYYTPTIMLIALLVVVVPPLFFGGDW-------- 357

Query: 391 SSLACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGHVLDALSSCHT 450
                   VY+ L L+V   PC+L ++ P++   AI + A+ G+L+KGG  L+ +     
Sbjct: 358 -----DTWVYQGLSLLVVGCPCSLVISTPVSIVSAIGNSAKNGVLVKGGIYLEEIGGLQA 417

Query: 451 IAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHP 510
           IAFDKTGTLT G  +     P   H   +N               +L++  A+E  + HP
Sbjct: 418 IAFDKTGTLTKGKPVVTDFIPYSEHMDEQN---------------SLSIITALETMSQHP 477

Query: 511 IGRAVVDHSL--GKDLPSFSVQNLEYFPGRGLVAMLQGIESGTEGGKLLKASLGSLDFIT 570
           +  A++  ++    D  S  + N     G+G+   + GI       KL ++SL     I+
Sbjct: 478 LASAIISKAMIDNVDYKSIEIDNFSSITGKGVKGEVNGITYYIGSSKLFESSLEKSQSIS 537

Query: 571 SLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIA 630
             Y+S  K  K     +   +G+E           + +I + D        +IA+L  + 
Sbjct: 538 QTYQSLQKQGK-----TAMLFGTE--------SNILAIIAVADEVRESSKEVIAQLHKLG 597

Query: 631 KLHVMMLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGIND 690
             H +MLTGD+  +A+ + K +G+ ++   L PEDKL+++K + + + G + M+G+G+ND
Sbjct: 598 IAHTIMLTGDNNDTAQFIGKEIGVSDIKAELMPEDKLTYIKELKQTY-GKVAMIGDGVND 657

Query: 691 APALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCISKSRQTTALVKQNASLALF 750
           APALAA+TVGI +    + TA+  ADV L+ D++  +PF ++ SR+T  ++KQN + +L 
Sbjct: 658 APALAASTVGIAMGGAGTDTALETADVALMGDDLKKLPFIVNLSRKTLKIIKQNITFSLG 703

Query: 751 SIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 786
              +A L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 718 IKLLALLLVLPGWLTLWIAIVADMGATLLVTLNGLRLM 703

BLAST of CmoCh02G005080 vs. ExPASy Swiss-Prot
Match: Q60048 (Probable cadmium-transporting ATPase OS=Listeria monocytogenes OX=1639 GN=cadA PE=1 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.1e-72
Identity = 200/693 (28.86%), Postives = 352/693 (50.79%), Query Frame = 0

Query: 102 FAKAIRWTDLANYLREHLQLCCCSMALFVAAAVCPYLV-PKPFVKPCQNALIAIAFPLVG 161
           F     +TD  +++R++ +L      LF+A      ++  + F     NAL   A  + G
Sbjct: 72  FTDPEHFTDHQSFIRKNWRLLL--SGLFIAVGYASQIMNGEDFY--LTNALFIFAIFIGG 131

Query: 162 VSSSLDALTDISGGKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAM 221
            S   +   ++   +  +  LM +A   + F+G   EG I++ +F +S   E Y + +A 
Sbjct: 132 YSLFKEGFKNLLKFEFTMETLMTIAIIGAAFIGEWAEGSIVVILFAVSEALERYSMDKAR 191

Query: 222 IDVKELKESYPDSALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEV 281
             ++ L +  P  ALV  +   ++          V V D+Q+   +++  G+ + +D  V
Sbjct: 192 QSIRSLMDIAPKEALVRRSGTDRM----------VHVDDIQIGDIMIIKPGQKIAMDGHV 251

Query: 282 FQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEE 341
            +G + V    +TGE  P+E  + D V  G  N +G + V  TK  +++T+S+I++L EE
Sbjct: 252 VKGYSAVNQAAITGESIPVEKNIDDSVFAGTLNEEGLLEVAVTKRVEDTTISKIIHLVEE 311

Query: 342 AQLNKPRLQRWLDEFGEHYSKAVVVLSIAVALVGPMLF--KWPFIGTSGSVTSSLACRGS 401
           AQ  +   Q ++D F ++Y+ A++V++  +A V P+LF   W                  
Sbjct: 312 AQGERAPAQAFVDTFAKYYTPAIIVIAALIATVPPLLFGGNW---------------ETW 371

Query: 402 VYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGT 461
           VY+ L ++V   PCAL V+ P+A   AI + A+ G+L+KGG  L+ +     IAFDKTGT
Sbjct: 372 VYQGLSVLVVGCPCALVVSTPVAIVTAIGNAAKNGVLVKGGVYLEEIGGLKAIAFDKTGT 431

Query: 462 LTTG-SLIFKAIEPIYGHQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVD 521
           LT G  ++   IE      ++ N++                + AA+E+ + HP+  A++ 
Sbjct: 432 LTKGVPVVTDYIELTEATNIQHNKN--------------YIIMAALEQLSQHPLASAIIK 491

Query: 522 HSLGK--DLPSFSVQNLEYFPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYK--S 581
           +   +  DL S +V +     G+G+   + G  +   G  +L   L +  F  S+++  S
Sbjct: 492 YGETREMDLTSINVNDFTSITGKGIRGTVDG-NTYYVGSPVLFKELLASQFTDSIHRQVS 551

Query: 582 DTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVM 641
           D + K      +   +G+            ++++ + D        +I  L ++     +
Sbjct: 552 DLQLK----GKTAMLFGTN--------QKLISIVAVADEVRSSSQHVIKRLHELGIEKTI 611

Query: 642 MLTGDHESSARRVAKAVGIKEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALA 701
           MLTGD++++A+ + + VG+ E+   L P+DKL ++K + + + G + MVG+GINDAPALA
Sbjct: 612 MLTGDNQATAQAIGQQVGVSEIEGELMPQDKLDYIKQL-KINFGKVAMVGDGINDAPALA 671

Query: 702 AATVGIVLAQRASATAIAVADVLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIA 761
           AATVGI +    + TAI  ADV L+ D++  +PF +  SR+T  ++KQN + +L    IA
Sbjct: 672 AATVGIAMGGAGTDTAIETADVALMGDDLQKLPFTVKLSRKTLQIIKQNITFSLVIKLIA 707

Query: 762 SLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 786
            L  + G+L LW+ ++   G TLLV LN +R +
Sbjct: 732 LLLVIPGWLTLWIAIMADMGATLLVTLNGLRLM 707

BLAST of CmoCh02G005080 vs. ExPASy Swiss-Prot
Match: Q6GIX1 (Probable cadmium-transporting ATPase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=cadA PE=3 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.8e-69
Identity = 181/613 (29.53%), Postives = 315/613 (51.39%), Query Frame = 0

Query: 177 NIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALV 236
           ++  LM +A   +  +G   E  I++ +F +S   E + + RA   ++ L +  P  ALV
Sbjct: 160 DMKTLMTVAVIGAAIIGEWAEASIVVVLFAISEALERFSMDRARQSIRSLMDIAPKEALV 219

Query: 237 LDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEI 296
           +   +  +          + V D+ V   ++V  GE + +D  +  G + V    +TGE 
Sbjct: 220 MRNGQEIM----------IHVDDIAVGDIMIVKPGEKIAMDGIIINGVSAVNQAAITGES 279

Query: 297 KPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFG 356
            P+  TV D V  G  N +G + VK TK  +++T+S+I++L EEAQ  +   Q ++D+F 
Sbjct: 280 VPVAKTVDDEVFAGTLNEEGLLEVKITKYVEDTTISKIIHLVEEAQGERAPAQAFVDKFA 339

Query: 357 EHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAV 416
           ++Y+  ++V++  VA+V P+ F        GS  +       VY+ L ++V   PCAL +
Sbjct: 340 KYYTPIIMVIAALVAVVPPLFF-------GGSWDT------WVYQGLAVLVVGCPCALVI 399

Query: 417 A-PLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQ 476
           + P++   AI + A+KG+L+KGG  L+ L +   IAFDKTGTLT      K +  +   +
Sbjct: 400 STPISIVSAIGNAAKKGVLIKGGVYLEELGAIKAIAFDKTGTLT------KGVPVVTDFK 459

Query: 477 VRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDL--PSFSVQNLEY 536
           V  ++ +         E+E  ++  A+E  + HP+  A++  +   ++      V++   
Sbjct: 460 VLNDQVE---------EKELFSIITALEYRSQHPLASAIMKKAEQDNITYSDVRVEDFTS 519

Query: 537 FPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFV 596
             GRG+   + G        +L K  L   DF       + K K ++    T+       
Sbjct: 520 ITGRGIQGNIDGTTYYIGSPRLFK-ELNVSDFSLEF---ENKVKVLQNQGKTA------- 579

Query: 597 HAALAVDGKVT-LIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIK 656
              +  D  +  +I + D       ++I +L  +     +MLTGD++ +A  +   VG+ 
Sbjct: 580 -MIIGTDQTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTAEAIGAHVGVS 639

Query: 657 EVYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVA 716
           ++   L P+DKL ++K +  EH G + M+G+G+NDAPALAA+TVGI +    + TAI  A
Sbjct: 640 DIQSELMPQDKLDYIKKMKAEH-GNVAMIGDGVNDAPALAASTVGIAMGGAGTDTAIETA 699

Query: 717 DVLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEG 776
           D+ L+ D++S +PF +  SR+T  ++K N + A+    IA L  + G+L LW+ +L   G
Sbjct: 700 DIALMGDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLTLWIAILSDMG 721

Query: 777 GTLLVCLNSIRAL 786
            T+LV LNS+R +
Sbjct: 760 ATILVALNSLRLM 721

BLAST of CmoCh02G005080 vs. ExPASy Swiss-Prot
Match: P20021 (Cadmium-transporting ATPase OS=Staphylococcus aureus OX=1280 GN=cadA PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.4e-68
Identity = 191/623 (30.66%), Postives = 319/623 (51.20%), Query Frame = 0

Query: 177 NIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALV 236
           ++  LM +A   +  +G   E  I++ +F +S   E + + R+   ++ L +  P  ALV
Sbjct: 161 DMKTLMTVAVIGATIIGKWAEASIVVILFAISEALERFSMDRSRQSIRSLMDIAPKEALV 220

Query: 237 LDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEI 296
               +  +          + V D+ V   ++V  GE + +D  +  G + V    +TGE 
Sbjct: 221 RRNGQEII----------IHVDDIAVGDIMIVKPGEKIAMDGIIVNGLSAVNQAAITGES 280

Query: 297 KPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFG 356
            P+   V D V  G  N +G I VK TK  +++T+++I++L EEAQ  +   Q ++D+F 
Sbjct: 281 VPVSKAVDDEVFAGTLNEEGLIEVKITKYVEDTTITKIIHLVEEAQGERAPAQAFVDKFA 340

Query: 357 EHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAV 416
           ++Y+  ++V++  VA+V P+ F        GS  +       VY+ L ++V   PCAL +
Sbjct: 341 KYYTPIIMVIAALVAVVPPLFF-------GGSWDT------WVYQGLAVLVVGCPCALVI 400

Query: 417 A-PLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQ 476
           + P++   AI + A+KG+L+KGG  L+ L +  T+AFDKTGTLT G  +    E +   Q
Sbjct: 401 STPISIVSAIGNAAKKGVLVKGGVYLEKLGAIKTVAFDKTGTLTKGVPVVTDFE-VLNDQ 460

Query: 477 VRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEY-- 536
           V               E+E  ++  A+E  + HP+  A++  +   ++P  +VQ  E+  
Sbjct: 461 VE--------------EKELFSIITALEYRSQHPLASAIMKKAEQDNIPYSNVQVEEFTS 520

Query: 537 FPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFV 596
             GRG    ++GI +GT         +GS      L  SD             S G E  
Sbjct: 521 ITGRG----IKGIVNGT------TYYIGSPKLFKELNVSD------------FSLGFENN 580

Query: 597 HAALAVDGKVTLIHLEDRPHPGVSSIIAELTD-----IAKLH------VMMLTGDHESSA 656
              L   GK  +I   ++   GV ++  E+ +     I KLH       +MLTGD++ +A
Sbjct: 581 VKILQNQGKTAMIIGTEKTILGVIAVADEVRETSKNVIQKLHQLGIKQTIMLTGDNQGTA 640

Query: 657 RRVAKAVGIKEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQ 716
             +   VG+ ++   L P+DKL ++K +  E+   + M+G+G+NDAPALAA+TVGI +  
Sbjct: 641 NAIGTHVGVSDIQSELMPQDKLDYIKKMQSEY-DNVAMIGDGVNDAPALAASTVGIAMGG 700

Query: 717 RASATAIAVADVLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLP 776
             + TAI  AD+ L+ D++S +PF +  SR+T  ++K N + A+    IA L  + G+L 
Sbjct: 701 AGTDTAIETADIALMGDDLSKLPFAVRLSRKTLNIIKANITFAIGIKIIALLLVIPGWLT 722

Query: 777 LWLTVLLHEGGTLLVCLNSIRAL 786
           LW+ +L   G T+LV LNS+R +
Sbjct: 761 LWIAILSDMGATILVALNSLRLM 722

BLAST of CmoCh02G005080 vs. ExPASy TrEMBL
Match: A0A6J1G7D7 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111451500 PE=3 SV=1)

HSP 1 Score: 1574.3 bits (4075), Expect = 0.0e+00
Identity = 817/824 (99.15%), Postives = 817/824 (99.15%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE
Sbjct: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV
Sbjct: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 817

BLAST of CmoCh02G005080 vs. ExPASy TrEMBL
Match: A0A6J1KX68 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499582 PE=3 SV=1)

HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 804/824 (97.57%), Postives = 809/824 (98.18%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFP LHLRRLVLCATAAND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNHDEHHRVH HHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGG+LLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGVLLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSY+KVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYKKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           Y IAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVREN+
Sbjct: 421 YTIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENK 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SD GSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDFGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           A LQGIESGTEGGKLLKASLGSLDFITSLY+SDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 ATLQGIESGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           SIR+LNNPSWSWRQD SQLLLKLK RGS PSLNTISTTVHSSPV
Sbjct: 781 SIRSLNNPSWSWRQDFSQLLLKLKFRGSQPSLNTISTTVHSSPV 817

BLAST of CmoCh02G005080 vs. ExPASy TrEMBL
Match: A0A6J1D720 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111017943 PE=3 SV=1)

HSP 1 Score: 1370.1 bits (3545), Expect = 0.0e+00
Identity = 719/824 (87.26%), Postives = 752/824 (91.26%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPI+S K RL  SR+RIAFS P L  S PS AF V N PNLHL RLV C  AA D
Sbjct: 1   MGTLSFPISSPKSRLPLSRNRIAFSTPFLPTSLPSHAFRVRNLPNLHL-RLVPCTAAAAD 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
            SNH        HHH HHHHHGHHHCDH  ELT PQRA++GFAKAIRWTDLANYLREHLQ
Sbjct: 61  QSNH--------HHHHHHHHHGHHHCDHGVELTAPQRAVVGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKP VKP QNA I +AFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPIVKPLQNAFIVVAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGN+LEGG+LL MFNLSHIAEEYF SR+MIDVKELKE++PDSALVLDT+
Sbjct: 181 LMALAAFASIFMGNSLEGGLLLAMFNLSHIAEEYFTSRSMIDVKELKENHPDSALVLDTD 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           +GKL NI DLSYQKVPVHDVQV+SY+LVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 DGKLSNITDLSYQKVPVHDVQVNSYILVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIA+ALVGP++FKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAIALVGPIMFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           YAIAISSCARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+VREN 
Sbjct: 421 YAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHKVRENR 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SD G CCIPCCE EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDFGPCCIPCCETEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           A L G ESGT GGKLLKASLGSLDFITSL+KS+ KSK+IKEA STSSYGSEFVHAALAVD
Sbjct: 541 ATLDGTESGTRGGKLLKASLGSLDFITSLHKSEIKSKEIKEAVSTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHP VSS+I EL D+AKLHVMMLTGDHESSA RVAKAVGI EV+FSLKP
Sbjct: 601 GKVTLIHLEDRPHPRVSSVIVELRDVAKLHVMMLTGDHESSAWRVAKAVGINEVHFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFC++KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCVAKSRQTTALVKQNASLALFSIFLASLPAVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNT--ISTTVHSS 823
           SIRALN+PSWSWRQDL QLL+ LK RGS PSL+T   STTV SS
Sbjct: 781 SIRALNDPSWSWRQDLKQLLMGLKFRGSQPSLDTSSTSTTVQSS 808

BLAST of CmoCh02G005080 vs. ExPASy TrEMBL
Match: A0A5A7VJB5 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold21G005290 PE=3 SV=1)

HSP 1 Score: 1335.9 bits (3456), Expect = 0.0e+00
Identity = 707/835 (84.67%), Postives = 748/835 (89.58%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRI-AFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAN 60
           MGTLSFPI SSK  LL + + +  F +P LQ SF SS F   NFP LHLRR VLC+ AA 
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILHLRRRVLCSAAAA 60

Query: 61  DHSNHDEHHRVHNHHHG--------HHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDL 120
             SNHD+HH VH+H+HG        HHHHH HHH DHD EL+GPQ+A+IGFAKA RWTDL
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHHHHHHGDHDVELSGPQKAVIGFAKATRWTDL 120

Query: 121 ANYLREHLQLCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDI 180
           ANYLRE LQLCCCSMALFVAAAVCPYLVPKP VKP Q A I I FPLVGVSS+LDALTDI
Sbjct: 121 ANYLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVGVSSALDALTDI 180

Query: 181 SGGKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYP 240
           SGGKVNIHVLMALAAFASIFMGNALEGG+LL MFNLSHIAEEYF SRAMIDVKELKE+YP
Sbjct: 181 SGGKVNIHVLMALAAFASIFMGNALEGGLLLAMFNLSHIAEEYFTSRAMIDVKELKENYP 240

Query: 241 DSALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEH 300
           D ALVLDTN+G++PNI DLSYQKVPV DV+VDSY+LVGAGE VPVDCEVFQGSATVTIEH
Sbjct: 241 DFALVLDTNDGEVPNITDLSYQKVPVQDVEVDSYILVGAGEFVPVDCEVFQGSATVTIEH 300

Query: 301 LTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW 360
           LTGEIKPLEITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW
Sbjct: 301 LTGEIKPLEITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRW 360

Query: 361 LDEFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASP 420
           LDEFGEHYSKAVVVL+  +ALVGP+LFKWPFIGTS       ACRGSVYRALGLMVAASP
Sbjct: 361 LDEFGEHYSKAVVVLATTIALVGPILFKWPFIGTS-------ACRGSVYRALGLMVAASP 420

Query: 421 CALAVAPLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPI 480
           CALAVAPLAYAIAISSCARKGILLKGG VLDAL++CHTIAFDKTGTLTTG L FKAIEPI
Sbjct: 421 CALAVAPLAYAIAISSCARKGILLKGGRVLDALAACHTIAFDKTGTLTTGGLFFKAIEPI 480

Query: 481 YGHQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNL 540
           YGH+V EN+SD GSCCIPCCE+EALAVAAAMEKGTTHPIGRAVV HS+GK LPSFSVQ+L
Sbjct: 481 YGHEVGENKSDFGSCCIPCCEDEALAVAAAMEKGTTHPIGRAVVGHSMGKVLPSFSVQSL 540

Query: 541 EYFPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSE 600
           EYFPGRGLVA L G ES   GGKLLKASLGSLDFITS YKS TKSK+IKEAA+TSSYGSE
Sbjct: 541 EYFPGRGLVATLHGTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSE 600

Query: 601 FVHAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGI 660
           FVHAALAVDGKVTLIHLEDRPHPGVSS+I+ELTD A+LHVMMLTGDHESSARRVA AVGI
Sbjct: 601 FVHAALAVDGKVTLIHLEDRPHPGVSSVISELTDRARLHVMMLTGDHESSARRVANAVGI 660

Query: 661 KEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAV 720
           KEV+FSLKPEDKLSHVKSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAV
Sbjct: 661 KEVHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAV 720

Query: 721 ADVLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHE 780
           ADVLLLRDNISGVPFCI+KSRQTTALVKQNASLALF IF+ASLP VLGFLPLWLTVLLHE
Sbjct: 721 ADVLLLRDNISGVPFCIAKSRQTTALVKQNASLALFFIFVASLPAVLGFLPLWLTVLLHE 780

Query: 781 GGTLLVCLNSIRALNNPSWSWR--QDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           GGTLLVCLNSIRALNNPSWSW   QDL Q L++LK +GS P LNT STTV SSPV
Sbjct: 781 GGTLLVCLNSIRALNNPSWSWSWGQDLKQFLMELKFKGSQPRLNTSSTTVQSSPV 825

BLAST of CmoCh02G005080 vs. ExPASy TrEMBL
Match: A0A5D3BGM3 (Putative cadmium/zinc-transporting ATPase HMA1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G001560 PE=3 SV=1)

HSP 1 Score: 1331.2 bits (3444), Expect = 0.0e+00
Identity = 705/833 (84.63%), Postives = 746/833 (89.56%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRI-AFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAN 60
           MGTLSFPI SSK  LL + + +  F +P LQ SF SS F   NFP L LRR VLC+ AA 
Sbjct: 1   MGTLSFPIPSSKSHLLSNPNPVPLFRHPFLQSSFSSSPFPSRNFPILRLRRRVLCSAAAA 60

Query: 61  DHSNHDEHHRVHNHHHGHHHHHGH------HHCDHDAELTGPQRALIGFAKAIRWTDLAN 120
             SNHD+HH VH+H+HGHHHHH H      HH DHD EL+GPQ+A+IGFAKA RWTDLA 
Sbjct: 61  GRSNHDDHHHVHDHNHGHHHHHHHHHHHHRHHGDHDVELSGPQKAVIGFAKATRWTDLAK 120

Query: 121 YLREHLQLCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISG 180
           YLRE LQLCCCSMALFVAAAVCPYLVPKP VKP Q A I I FPLVGVSS+LDALTDISG
Sbjct: 121 YLRESLQLCCCSMALFVAAAVCPYLVPKPIVKPLQIAFIVIGFPLVGVSSALDALTDISG 180

Query: 181 GKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDS 240
           GKVNIHVLMALAAFASIFMGNALEGG+LL MFNLSHIAEEYF SRAMIDVKELKE+YPD 
Sbjct: 181 GKVNIHVLMALAAFASIFMGNALEGGLLLAMFNLSHIAEEYFTSRAMIDVKELKENYPDF 240

Query: 241 ALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLT 300
           ALVLDTN+G++PNI DLSYQKVPV DV+VDSY+LVGAGE VPVDCEVFQGSATVTIEHLT
Sbjct: 241 ALVLDTNDGEVPNITDLSYQKVPVQDVEVDSYILVGAGEFVPVDCEVFQGSATVTIEHLT 300

Query: 301 GEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 360
           GEIKPLEITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD
Sbjct: 301 GEIKPLEITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 360

Query: 361 EFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCA 420
           EFGEHYSKAVVVL+  +ALVGP+LFKWPFIGTS       ACRGSVYRALGLMVAASPCA
Sbjct: 361 EFGEHYSKAVVVLATTIALVGPILFKWPFIGTS-------ACRGSVYRALGLMVAASPCA 420

Query: 421 LAVAPLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYG 480
           LAVAPLAYAIAISSCARKGILLKGG VLDAL++CHTIAFDKTGTLTTG L FKAIEPIYG
Sbjct: 421 LAVAPLAYAIAISSCARKGILLKGGRVLDALAACHTIAFDKTGTLTTGGLFFKAIEPIYG 480

Query: 481 HQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEY 540
           H+V EN+SD GSCCIPCCE+EALAVAAAMEKGTTHPIGRAVV HS+GK LPSFSVQ+LEY
Sbjct: 481 HEVGENKSDFGSCCIPCCEDEALAVAAAMEKGTTHPIGRAVVGHSMGKVLPSFSVQSLEY 540

Query: 541 FPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFV 600
           FPGRGLVA L G ES   GGKLLKASLGSLDFITS YKS TKSK+IKEAA+TSSYGSEFV
Sbjct: 541 FPGRGLVATLHGTES---GGKLLKASLGSLDFITSCYKSGTKSKEIKEAANTSSYGSEFV 600

Query: 601 HAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKE 660
           HAALAVDGKVTLIHLEDRPHPGVSS+I+ELTD A+LHVMMLTGDHESSARRVA AVGIKE
Sbjct: 601 HAALAVDGKVTLIHLEDRPHPGVSSVISELTDRARLHVMMLTGDHESSARRVANAVGIKE 660

Query: 661 VYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 720
           V+FSLKPEDKLSHVKSISRE+GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD
Sbjct: 661 VHFSLKPEDKLSHVKSISRENGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 720

Query: 721 VLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGG 780
           VLLLRDNISGVPFCI+KSRQTTALVKQNASLALF IF+ASLP VLGFLPLWLTVLLHEGG
Sbjct: 721 VLLLRDNISGVPFCIAKSRQTTALVKQNASLALFFIFVASLPAVLGFLPLWLTVLLHEGG 780

Query: 781 TLLVCLNSIRALNNPSWSWR--QDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           TLLVCLNSIRALNNPSWSW   QDL Q L++LK +GS P LNT STTV SSPV
Sbjct: 781 TLLVCLNSIRALNNPSWSWSWGQDLKQFLMELKFKGSQPRLNTSSTTVQSSPV 823

BLAST of CmoCh02G005080 vs. NCBI nr
Match: XP_022947718.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1574.3 bits (4075), Expect = 0.0e+00
Identity = 817/824 (99.15%), Postives = 817/824 (99.15%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE
Sbjct: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV
Sbjct: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 817

BLAST of CmoCh02G005080 vs. NCBI nr
Match: XP_023006942.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 804/824 (97.57%), Postives = 809/824 (98.18%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFP LHLRRLVLCATAAND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPYLHLRRLVLCATAAND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNHDEHHRVH HHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHYHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGG+LLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGVLLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSY+KVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYKKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           Y IAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVREN+
Sbjct: 421 YTIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENK 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SD GSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDFGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           A LQGIESGTEGGKLLKASLGSLDFITSLY+SDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 ATLQGIESGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           SIR+LNNPSWSWRQD SQLLLKLK RGS PSLNTISTTVHSSPV
Sbjct: 781 SIRSLNNPSWSWRQDFSQLLLKLKFRGSQPSLNTISTTVHSSPV 817

BLAST of CmoCh02G005080 vs. NCBI nr
Match: XP_023533519.1 (LOW QUALITY PROTEIN: probable cadmium/zinc-transporting ATPase HMA1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1531.5 bits (3964), Expect = 0.0e+00
Identity = 801/826 (96.97%), Postives = 806/826 (97.58%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATA ND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAVND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNH+EHHRVHNHH  HHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHEEHHRVHNHH--HHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPK FVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKLFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVREN+
Sbjct: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENK 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SD GSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV
Sbjct: 481 SDFGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
           AMLQGIESGTEGGKLLKASLGSLDFITSLY+SDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYESDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGL--IMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR 720
           EDKLSHVKSISREHG  +   MVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR
Sbjct: 661 EDKLSHVKSISREHGXQMTFXMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLR 720

Query: 721 DNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVC 780
           DNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVC
Sbjct: 721 DNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVC 780

Query: 781 LNSIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           LNSIRALNNPSWSWRQ+ SQLLLKLK RGS PSLNTISTTVHSSPV
Sbjct: 781 LNSIRALNNPSWSWRQNFSQLLLKLKFRGSQPSLNTISTTVHSSPV 817

BLAST of CmoCh02G005080 vs. NCBI nr
Match: KAG6605188.1 (putative cadmium/zinc-transporting ATPase HMA1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 767/824 (93.08%), Postives = 769/824 (93.33%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60
           MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND
Sbjct: 1   MGTLSFPIASSKPRLLPSRSRIAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAND 60

Query: 61  HSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120
           HSNHDEHHRVHNHHH HHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ
Sbjct: 61  HSNHDEHHRVHNHHHHHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHLQ 120

Query: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180
           LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV
Sbjct: 121 LCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIHV 180

Query: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240
           LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN
Sbjct: 181 LMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDTN 240

Query: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLE 300
           EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVF GSATVTIEHLTGEIKPLE
Sbjct: 241 EGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFHGSATVTIEHLTGEIKPLE 300

Query: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360
           ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS
Sbjct: 301 ITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYS 360

Query: 361 KAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPLA 420
           KAVVVLSIAVALVGPMLFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPLA
Sbjct: 361 KAVVVLSIAVALVGPMLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPLA 420

Query: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENE 480
           YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVREN+
Sbjct: 421 YAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENK 480

Query: 481 SDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLV 540
           SDSGSCCIPCCEEEALAVAAAMEK                                    
Sbjct: 481 SDSGSCCIPCCEEEALAVAAAMEK------------------------------------ 540

Query: 541 AMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600
                   GTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD
Sbjct: 541 --------GTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAVD 600

Query: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660
           GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP
Sbjct: 601 GKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLKP 660

Query: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720
           EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN
Sbjct: 661 EDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRDN 720

Query: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 780
           ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN
Sbjct: 721 ISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLN 773

Query: 781 SIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           SIRALNNPSWSWRQDLSQLLLKLK RGS PSL+TISTTVHSSPV
Sbjct: 781 SIRALNNPSWSWRQDLSQLLLKLKFRGSQPSLDTISTTVHSSPV 773

BLAST of CmoCh02G005080 vs. NCBI nr
Match: XP_038900941.1 (probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900942.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida] >XP_038900943.1 probable cadmium/zinc-transporting ATPase HMA1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1375.5 bits (3559), Expect = 0.0e+00
Identity = 722/825 (87.52%), Postives = 759/825 (92.00%), Query Frame = 0

Query: 1   MGTLSFPIASSKPRLLPSRSR-IAFSNPSLQPSFPSSAFSVPNFPNLHLRRLVLCATAAN 60
           MGTLSFPIASSK RL  + +  IAFSNP LQ SF SS F  PNFP LHLRR VLC+ A  
Sbjct: 1   MGTLSFPIASSKSRLSSTPNHPIAFSNPFLQSSFSSSPFRPPNFPILHLRRRVLCSAAVT 60

Query: 61  DHSNHDEHHRVHNHHHGHHHHHGHHHCDHDAELTGPQRALIGFAKAIRWTDLANYLREHL 120
             SNHD+H  VH+HHH HHH H HHHCDHD EL+GPQ+A+IGFAKAIR TDLANYLREHL
Sbjct: 61  GRSNHDDHQHVHDHHH-HHHRHAHHHCDHDVELSGPQKAVIGFAKAIRLTDLANYLREHL 120

Query: 121 QLCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISGGKVNIH 180
           QLCCCSMALFVAAAVCPY VPKP VKP QNA I IAFPLVGVSSSLDALTDISGGKVNIH
Sbjct: 121 QLCCCSMALFVAAAVCPYFVPKPIVKPLQNAFIVIAFPLVGVSSSLDALTDISGGKVNIH 180

Query: 181 VLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDSALVLDT 240
           VLMALAAFASIFMGNALEGG+LL MFNLSHIAEE+F SRAMIDVKELKE+YPDSALVLDT
Sbjct: 181 VLMALAAFASIFMGNALEGGLLLAMFNLSHIAEEHFTSRAMIDVKELKENYPDSALVLDT 240

Query: 241 NEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPL 300
           N+GKLPN  DLSYQKVPVHDVQVDSY+LVGAGE VPVDCEVFQGSATVTIEHLTGEIKPL
Sbjct: 241 NDGKLPNSTDLSYQKVPVHDVQVDSYILVGAGEFVPVDCEVFQGSATVTIEHLTGEIKPL 300

Query: 301 EITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHY 360
           EITVG RVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHY
Sbjct: 301 EITVGSRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHY 360

Query: 361 SKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCALAVAPL 420
           SKAVVVL+IA+ALVGP+LFKWPFIGTS       ACRGSVYRALGLMVAASPCALAVAPL
Sbjct: 361 SKAVVVLAIAIALVGPVLFKWPFIGTS-------ACRGSVYRALGLMVAASPCALAVAPL 420

Query: 421 AYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVREN 480
           AYAIAISSCARKGILLKGG VLDAL+SCHTIAFDKTGTLTTG LIFKAIEPIYGH+V EN
Sbjct: 421 AYAIAISSCARKGILLKGGRVLDALASCHTIAFDKTGTLTTGGLIFKAIEPIYGHEVGEN 480

Query: 481 ESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGL 540
           +S+ GSCC PCCEEEALAVAAAMEKGTTHPIGRAVVDHS+GKDLPSFSVQNLEYFPGRGL
Sbjct: 481 KSEFGSCCNPCCEEEALAVAAAMEKGTTHPIGRAVVDHSMGKDLPSFSVQNLEYFPGRGL 540

Query: 541 VAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFVHAALAV 600
           +A L G ESGTEG K LKASLGSLDFITS Y+S++KS++IKEAA+TSSYGSEFVHAALAV
Sbjct: 541 IATLHGSESGTEGRKRLKASLGSLDFITSCYQSESKSREIKEAANTSSYGSEFVHAALAV 600

Query: 601 DGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKEVYFSLK 660
           DGKVTLIHLEDRPHPGVSS+IAELTD AKLHVMMLTGDHESSARRVA AVGIKEV+FSLK
Sbjct: 601 DGKVTLIHLEDRPHPGVSSVIAELTDRAKLHVMMLTGDHESSARRVANAVGIKEVHFSLK 660

Query: 661 PEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLRD 720
           PEDKLSHVKSISRE+ GGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLL D
Sbjct: 661 PEDKLSHVKSISREN-GGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVADVLLLWD 720

Query: 721 NISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCL 780
           NISGVPFCI+KSRQTTALVKQNASLALFSIF+ASLP VLGFLPLWLTVLLHEGGTLLVCL
Sbjct: 721 NISGVPFCIAKSRQTTALVKQNASLALFSIFVASLPAVLGFLPLWLTVLLHEGGTLLVCL 780

Query: 781 NSIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVHSSPV 825
           NSIRALNNPSWSW+QDL QLL++LK +GS P LNT STTV SSPV
Sbjct: 781 NSIRALNNPSWSWKQDLKQLLMELKFKGSQPRLNTSSTTVQSSPV 816

BLAST of CmoCh02G005080 vs. TAIR 10
Match: AT4G37270.1 (heavy metal atpase 1 )

HSP 1 Score: 1066.6 bits (2757), Expect = 9.9e-312
Identity = 553/767 (72.10%), Postives = 645/767 (84.09%), Query Frame = 0

Query: 57  AANDHSNHDEHHRVHNHHHGHHHHHGHHHC---DHDAELTGPQRALIGFAKAIRWTDLAN 116
           A  DH +HD HH     HH HHHHH  H C   +  AE + PQ+ L GFAKAI W  LAN
Sbjct: 63  AVEDH-HHDHHHDDEQDHHNHHHHHHQHGCCSVELKAE-SKPQKMLFGFAKAIGWVRLAN 122

Query: 117 YLREHLQLCCCSMALFVAAAVCPYLVPKPFVKPCQNALIAIAFPLVGVSSSLDALTDISG 176
           YLREHL LCC + A+F+AAAVCPYL P+P++K  QNA + + FPLVGVS+SLDAL DI+G
Sbjct: 123 YLREHLHLCCSAAAMFLAAAVCPYLAPEPYIKSLQNAFMIVGFPLVGVSASLDALMDIAG 182

Query: 177 GKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHIAEEYFISRAMIDVKELKESYPDS 236
           GKVNIHVLMALAAFAS+FMGNALEGG+LL MFNL+HIAEE+F SR+M+DVKELKES PDS
Sbjct: 183 GKVNIHVLMALAAFASVFMGNALEGGLLLAMFNLAHIAEEFFTSRSMVDVKELKESNPDS 242

Query: 237 ALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLT 296
           AL+++ + G +PNI+DLSY+ VPVH V+V SY+LVG GE VPVDCEV+QGSAT+TIEHLT
Sbjct: 243 ALLIEVHNGNVPNISDLSYKSVPVHSVEVGSYVLVGTGEIVPVDCEVYQGSATITIEHLT 302

Query: 297 GEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLD 356
           GE+KPLE   GDRVPGGARNLDGR+IVKATK W +STL++IV LTEEA  NKP+LQRWLD
Sbjct: 303 GEVKPLEAKAGDRVPGGARNLDGRMIVKATKAWNDSTLNKIVQLTEEAHSNKPKLQRWLD 362

Query: 357 EFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVTSSLACRGSVYRALGLMVAASPCA 416
           EFGE+YSK VVVLS+A+A +GP LFKWPF+ T+       ACRGSVYRALGLMVAASPCA
Sbjct: 363 EFGENYSKVVVVLSLAIAFLGPFLFKWPFLSTA-------ACRGSVYRALGLMVAASPCA 422

Query: 417 LAVAPLAYAIAISSCARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYG 476
           LAVAPLAYA AISSCARKGILLKG  VLDAL+SCHTIAFDKTGTLTTG L  KAIEPIYG
Sbjct: 423 LAVAPLAYATAISSCARKGILLKGAQVLDALASCHTIAFDKTGTLTTGGLTCKAIEPIYG 482

Query: 477 HQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEY 536
           HQ   N S   +CCIP CE+EALAVAAAMEKGTTHPIGRAVVDHS+GKDLPS  V++ EY
Sbjct: 483 HQGGTNSSVI-TCCIPNCEKEALAVAAAMEKGTTHPIGRAVVDHSVGKDLPSIFVESFEY 542

Query: 537 FPGRGLVAMLQGIESGTEGGKLLKASLGSLDFITSLYKSDTKSKKIKEAASTSSYGSEFV 596
           FPGRGL A + G+++  E  +L KASLGS++FITSL+KS+ +SK+IK+A + SSYG +FV
Sbjct: 543 FPGRGLTATVNGVKTVAEESRLRKASLGSIEFITSLFKSEDESKQIKDAVNASSYGKDFV 602

Query: 597 HAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIKE 656
           HAAL+VD KVTLIHLED+P PGVS +IAEL   A+L VMMLTGDH+SSA RVA AVGI E
Sbjct: 603 HAALSVDQKVTLIHLEDQPRPGVSGVIAELKSWARLRVMMLTGDHDSSAWRVANAVGITE 662

Query: 657 VYFSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 716
           VY +LKPEDKL+HVK+I+RE GGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD
Sbjct: 663 VYCNLKPEDKLNHVKNIAREAGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIAVAD 722

Query: 717 VLLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLGFLPLWLTVLLHEGG 776
           +LLLRDNI+GVPFC++KSRQTT+LVKQN +LAL SIF+A+LP+VLGF+PLWLTVLLHEGG
Sbjct: 723 ILLLRDNITGVPFCVAKSRQTTSLVKQNVALALTSIFLAALPSVLGFVPLWLTVLLHEGG 782

Query: 777 TLLVCLNSIRALNNPSWSWRQDLSQLLLKLKLRGSHPSLNTISTTVH 821
           TLLVCLNS+R LN+PSWSW+QD+  L+ KL+ +    S +   ++ H
Sbjct: 783 TLLVCLNSVRGLNDPSWSWKQDIVHLINKLRSQEPTSSSSNSLSSAH 819

BLAST of CmoCh02G005080 vs. TAIR 10
Match: AT5G21930.1 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 203.4 bits (516), Expect = 7.2e-52
Identity = 190/614 (30.94%), Postives = 297/614 (48.37%), Query Frame = 0

Query: 200 ILLGMFNLSHIAEEYFISRAMIDVKELKESY-PDSALVLDTNEGKLPNIADLSYQ----K 259
           +LLG   L    EE    +A  D+ EL       S LV+ +++   P  + LS       
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 260 VPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNL 319
           V V D++V   LLV  GE+ PVD  V  G + V    LTGE  P+    G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 320 DGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAV---- 379
           DG + +KA+ T   ST+S+IV + E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 380 ALVGPMLFKWPFIG-TSGSVTSSLACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSC 439
             VG  +F    +   +G    +LA   S+  A+ ++V + PCAL +A P A  I  S  
Sbjct: 466 YYVGSHIFPDVLLNDIAGPDGDALAL--SLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 440 ARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCCI 499
           A++G L++GG VL+ L+S   +A DKTGTLT G  +   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 500 PCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVAMLQGIES 559
              E+E L +AAA+EK  THPI +A+V+ +   +L +   +     PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 560 GTEGGKLLKASLGSLDFITS--LYKSDTK---------SKKIKEAASTSSYGSEFVHAAL 619
                     ++GSL++++   L K+D+            K+   +STS Y    V+   
Sbjct: 646 -------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGR 705

Query: 620 AVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIK--EVY 679
             +G +  I + D         +A L +   +  ++L+GD E +   VAK VGIK     
Sbjct: 706 EGEGIIGAIAISDCLRQDAEFTVARLQE-KGIKTVLLSGDREGAVATVAKNVGIKSESTN 765

Query: 680 FSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIA-VADV 739
           +SL PE K   + ++ +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V
Sbjct: 766 YSLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASV 825

Query: 740 LLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLG-FLPLWLTVLLHEGG 788
           +L+R+ +S V   +S ++ T + V QN + A+ +  + S+P   G  LP +   +     
Sbjct: 826 ILVRNKLSHVVDALSLAQATMSKVYQNLAWAI-AYNVISIPIAAGVLLPQYDFAMTPSLS 867

BLAST of CmoCh02G005080 vs. TAIR 10
Match: AT5G21930.2 (P-type ATPase of Arabidopsis 2 )

HSP 1 Score: 203.4 bits (516), Expect = 7.2e-52
Identity = 190/614 (30.94%), Postives = 297/614 (48.37%), Query Frame = 0

Query: 200 ILLGMFNLSHIAEEYFISRAMIDVKELKESY-PDSALVLDTNEGKLPNIADLSYQ----K 259
           +LLG   L    EE    +A  D+ EL       S LV+ +++   P  + LS       
Sbjct: 286 MLLGFVLLGRSLEERAKLQASTDMNELLSLISTQSRLVITSSDNNTPVDSVLSSDSICIN 345

Query: 260 VPVHDVQVDSYLLVGAGESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNL 319
           V V D++V   LLV  GE+ PVD  V  G + V    LTGE  P+    G  V  G  N 
Sbjct: 346 VSVDDIRVGDSLLVLPGETFPVDGSVLAGRSVVDESMLTGESLPVFKEEGCSVSAGTINW 405

Query: 320 DGRIIVKATKTWKESTLSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAV---- 379
           DG + +KA+ T   ST+S+IV + E+AQ N   +QR  D     +   ++ LS       
Sbjct: 406 DGPLRIKASSTGSNSTISKIVRMVEDAQGNAAPVQRLADAIAGPFVYTIMSLSAMTFAFW 465

Query: 380 ALVGPMLFKWPFIG-TSGSVTSSLACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSC 439
             VG  +F    +   +G    +LA   S+  A+ ++V + PCAL +A P A  I  S  
Sbjct: 466 YYVGSHIFPDVLLNDIAGPDGDALAL--SLKLAVDVLVVSCPCALGLATPTAILIGTSLG 525

Query: 440 ARKGILLKGGHVLDALSSCHTIAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCCI 499
           A++G L++GG VL+ L+S   +A DKTGTLT G  +   +  + G++             
Sbjct: 526 AKRGYLIRGGDVLERLASIDCVALDKTGTLTEGRPVVSGVASL-GYE------------- 585

Query: 500 PCCEEEALAVAAAMEKGTTHPIGRAVVDHSLGKDLPSFSVQNLEYFPGRGLVAMLQGIES 559
              E+E L +AAA+EK  THPI +A+V+ +   +L +   +     PG G +A + G   
Sbjct: 586 ---EQEVLKMAAAVEKTATHPIAKAIVNEAESLNLKTPETRGQLTEPGFGTLAEIDG--- 645

Query: 560 GTEGGKLLKASLGSLDFITS--LYKSDTK---------SKKIKEAASTSSYGSEFVHAAL 619
                     ++GSL++++   L K+D+            K+   +STS Y    V+   
Sbjct: 646 -------RFVAVGSLEWVSDRFLKKNDSSDMVKLESLLDHKLSNTSSTSRYSKTVVYVGR 705

Query: 620 AVDGKVTLIHLEDRPHPGVSSIIAELTDIAKLHVMMLTGDHESSARRVAKAVGIK--EVY 679
             +G +  I + D         +A L +   +  ++L+GD E +   VAK VGIK     
Sbjct: 706 EGEGIIGAIAISDCLRQDAEFTVARLQE-KGIKTVLLSGDREGAVATVAKNVGIKSESTN 765

Query: 680 FSLKPEDKLSHVKSISREHGGGLIMVGEGINDAPALAAATVGIVLAQRASATAIA-VADV 739
           +SL PE K   + ++ +  G  + MVG+GINDAP+LA A VGI L   A   A +  A V
Sbjct: 766 YSLSPEKKFEFISNL-QSSGHRVAMVGDGINDAPSLAQADVGIALKIEAQENAASNAASV 825

Query: 740 LLLRDNISGVPFCISKSRQTTALVKQNASLALFSIFIASLPTVLG-FLPLWLTVLLHEGG 788
           +L+R+ +S V   +S ++ T + V QN + A+ +  + S+P   G  LP +   +     
Sbjct: 826 ILVRNKLSHVVDALSLAQATMSKVYQNLAWAI-AYNVISIPIAAGVLLPQYDFAMTPSLS 867

BLAST of CmoCh02G005080 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 200.3 bits (508), Expect = 6.1e-51
Identity = 176/640 (27.50%), Postives = 303/640 (47.34%), Query Frame = 0

Query: 152 IAIAFPLVGVSSSL-DALTDISGGKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHI 211
           +A+A    G+   L  A   I   +++I++L+ +   A++ M + +E   ++ +F +S  
Sbjct: 123 LAVAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDW 182

Query: 212 AEEYFISRAMIDVKELKESYPDSALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGA 271
            E     +A   ++ L    P  A++ +T E            +V V +V+VD+ + V A
Sbjct: 183 LETRASYKATSVMQSLMSLAPQKAIIAETGE------------EVEVDEVKVDTVVAVKA 242

Query: 272 GESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKEST 331
           GE++P+D  V  G+  V  + LTGE  P+       V  G  NL+G I VK T    +  
Sbjct: 243 GETIPIDGIVVDGNCEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCV 302

Query: 332 LSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVT 391
           ++++  L EEAQ +K + QR +D+  ++Y+ A++++S  VA+V P++ K   +       
Sbjct: 303 VAKMAKLVEEAQSSKTKSQRLIDKCSQYYTPAIILVSACVAIV-PVIMKVHNL------- 362

Query: 392 SSLACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGHVLDALSSCHT 451
                +   + AL ++V+  PC L ++ P+A   A++  A  G+L+K    LD LS    
Sbjct: 363 -----KHWFHLALVVLVSGCPCGLILSTPVATFCALTKAATSGLLIKSADYLDTLSKIKI 422

Query: 452 IAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHP 511
           +AFDKTGT+T G  I                 D  S          L   +++E  ++HP
Sbjct: 423 VAFDKTGTITRGEFIV---------------IDFKSLSRDINLRSLLYWVSSVESKSSHP 482

Query: 512 IGRAVVDH--SLGKDLPSFSVQNLEYFPGRGLVAMLQGIESGTEGGKLLKASLGSLDFIT 571
           +   +VD+  S+  +     V++ + FPG G+   + G +      K+  AS      + 
Sbjct: 483 MAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFIGNKKI--ASRAGCSTVP 542

Query: 572 SLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIA 631
            + + DTK  K        + G  +V   LA        +L D    GVS  +AEL  + 
Sbjct: 543 EI-EVDTKGGK--------TVGYVYVGERLA-----GFFNLSDACRSGVSQAMAELKSLG 602

Query: 632 KLHVMMLTGDHESSARRVAKAVG--IKEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGI 691
            +   MLTGD++++A    + +G  +  V+  L PEDK   ++   +E  G   MVG+G+
Sbjct: 603 -IKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKE--GPTAMVGDGV 662

Query: 692 NDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCISKSRQTTALVKQNASLA 751
           NDAPALA A +GI +    SA A    +++L+ ++I  +P  +  +R+    V +N  L+
Sbjct: 663 NDAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLS 703

Query: 752 LFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRAL 786
           +            G   +W  VL+  G  LLV  NS+  L
Sbjct: 723 IILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLL 703

BLAST of CmoCh02G005080 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 199.5 bits (506), Expect = 1.0e-50
Identity = 174/642 (27.10%), Postives = 312/642 (48.60%), Query Frame = 0

Query: 152 IAIAFPLVGVSSSL-DALTDISGGKVNIHVLMALAAFASIFMGNALEGGILLGMFNLSHI 211
           +A+A  + G+   L  A+  ++  +++I++L+ +   A+I M +  E  +++ +F ++  
Sbjct: 113 LAVAAVVAGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEW 172

Query: 212 AEEYFISRAMIDVKELKESYPDSALVLDTNEGKLPNIADLSYQKVPVHDVQVDSYLLVGA 271
            +     +A   ++ L    P  A++ +T E            +V V +++ ++ + V A
Sbjct: 173 LQSRASYKASAVMQSLMSLAPQKAVIAETGE------------EVEVDELKTNTVIAVKA 232

Query: 272 GESVPVDCEVFQGSATVTIEHLTGEIKPLEITVGDRVPGGARNLDGRIIVKATKTWKEST 331
           GE++P+D  V  G+  V  + LTGE  P+       V  G  NL+G I V  T   ++  
Sbjct: 233 GETIPIDGVVVDGNCEVDEKTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCV 292

Query: 332 LSRIVNLTEEAQLNKPRLQRWLDEFGEHYSKAVVVLSIAVALVGPMLFKWPFIGTSGSVT 391
           ++++  L EEAQ +K   QR++D+  ++Y+ A++++SI    +       PF     ++ 
Sbjct: 293 VAKMAKLVEEAQNSKTETQRFIDKCSKYYTPAIILISICFVAI-------PFALKVHNL- 352

Query: 392 SSLACRGSVYRALGLMVAASPCALAVA-PLAYAIAISSCARKGILLKGGHVLDALSSCHT 451
                +  V+ AL ++V+A PC L ++ P+A   A++  A  G+L+KG   L+ L+    
Sbjct: 353 -----KHWVHLALVVLVSACPCGLILSTPVATFCALTKAATSGLLIKGADYLETLAKIKI 412

Query: 452 IAFDKTGTLTTGSLIFKAIEPIYGHQVRENESDSGSCCIPCCEEEALAVAAAMEKGTTHP 511
           +AFDKTGT+T G  I           V + +S S    +    +  L   ++ E  ++HP
Sbjct: 413 VAFDKTGTITRGEFI-----------VMDFQSLSEDISL----QSLLYWVSSTESKSSHP 472

Query: 512 IGRAVVDH--SLGKDLPSFSVQNLEYFPGRGLVAMLQGIESGTEGGKLLKASLGSLDFIT 571
           +  AVVD+  S+  +    +V++ + FPG G+   + G E    G K + +  G L    
Sbjct: 473 MAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYI-GNKRIASRAGCLS--V 532

Query: 572 SLYKSDTKSKKIKEAASTSSYGSEFVHAALAVDGKVTLIHLEDRPHPGVSSIIAELTDIA 631
                DTK  K        + G  +V   LA      + +L D    GV+  + EL  + 
Sbjct: 533 PDIDVDTKGGK--------TIGYVYVGETLA-----GVFNLSDACRSGVAQAMKELKSLG 592

Query: 632 KLHVMMLTGDHESSARRVAKAVG--IKEVYFSLKPEDKLSHVKSISREHGGGLIMVGEGI 691
            + + MLTGD+ ++A    + +G  +  V   L PEDK   +K + RE  G   MVG+G+
Sbjct: 593 -IKIAMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREE-GPTAMVGDGL 652

Query: 692 NDAPALAAATVGIVLAQRASATAIAVADVLLLRDNISGVPFCISKSRQTTALVKQNASLA 751
           NDAPALA A +GI +    SA A    +++L+ ++I  +P  I  +++    V +N  ++
Sbjct: 653 NDAPALATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVIS 696

Query: 752 LFSIFIASLPTVLGFLPLWLTVLLHEGGTLLVCLNSIRALNN 788
           +            G   +W  VL   G  LLV LNS+  L++
Sbjct: 713 ITMKGAILALAFAGHPLIWAAVLADVGTCLLVILNSMLLLSD 696