CmoCh02G004540 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G004540
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Cmo_Chr02: 2371070 .. 2372038 (+)
RNA-Seq Expression	CmoCh02G004540
Synteny	CmoCh02G004540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGCGTGGAGTCCAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCCGGCGCTGGAAAAGGAGATCAGAAGCTCCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTTTACCACCAGCACCTCAACCCTTGGGTGGCTCAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGACGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGAGTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCTCATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATATATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAATCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGATAACCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTGGATGTGGTGGAAACCGACGGCGAAGGGCACGTGTTCCATTTGTTCAATCCAAGCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCGGTTGTTGA

mRNA sequence

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGCGTGGAGTCCAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCCGGCGCTGGAAAAGGAGATCAGAAGCTCCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTTTACCACCAGCACCTCAACCCTTGGGTGGCTCAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGACGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGAGTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCTCATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATATATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAATCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGATAACCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTGGATGTGGTGGAAACCGACGGCGAAGGGCACGTGTTCCATTTGTTCAATCCAAGCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCGGTTGTTGA

Coding sequence (CDS)

ATGGCTTCCGATGAAATTGCCTTGAACGCTGCACCCTGGTTCATCATTTACAAAGACCGTCGGATCGACAGGCTTATCGGCAACGACATCGATCCTCCCGGTCTTGATCCGAAAACCGGCGTGGAGTCCAAAGATGTCGTTATCTCGCAGGAAGATTCCGTCGCTGTCCGGATTTACAGGCCTAAATCTGCTGCCGGCGCTGGAAAAGGAGATCAGAAGCTCCCTCTTCTTGTTTACTTCCATGGCGGTGGGTTCTGCATCGAAACGGCGTTTTCTCCTTTTTACCACCAGCACCTCAACCCTTGGGTGGCTCAAGCCAACATCGTCGCTGTCTCCGTCAACTATCGACGCGCACCGGAGCACCAGCTACCGATTCCTTTTGAAGATTCATGGAGTGCTCTGAAATGGATCGCGGCTCACTCGAATGGAACCGGGCCAGATGAGTGGTTGAATGAAATTGCAGATCTGAACCAGGTTTATCTGGCTGGAGATAGCGCTGGAGGGAACATCGCCCACCGGATGGCGCTGCGGATGGCGACGGAGGGGCTGGCCGGAGTGAAGATCAAGGGATTGCAGTTGATTCACCCTCATTTTTGGGGGGGAAAATTGATCGGAGAAGAAAAGGAGTGGGATCCGAAGAGCATATATGTGGTGGAGAATCTGTGGTATTGGGTTTCGAAGGATATTAAGGATTTAGATGATCCGATTGTGAATCCGGAGCACGAACCGGATTTGGGGCGGCTTGCGGCGGAGAAAGTGGCGATTTATGTAGCGGAGAAGGATAACCTGAAGGAGAGGGGACGGCATTACGCGGAATGTTTGAGGAAATCAGGATGGGGCGGAACCGTGGATGTGGTGGAAACCGACGGCGAAGGGCACGTGTTCCATTTGTTCAATCCAAGCTGCGACTCGGCGGCAGACCTCGTCGGACAGCTCGCGGCGTTCATCAGCGGCAAGTGCGGTTGTTGA

Protein sequence

MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPSCDSAADLVGQLAAFISGKCGC

Homology

[Close]

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.2e-84
Identity = 159/321 (49.53%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
             A + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  --AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSW+ALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPSCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 5.8e-79
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V QAN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSW+ALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPSC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 7.6e-79
Identity = 154/324 (47.53%), Postives = 209/324 (64.51%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P+ A
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AGA-GKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQL 124
           A A  +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSW+ALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPSCDSAADLVGQLAAFISG 319
           FHL +P+ + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 8.4e-78
Identity = 149/313 (47.60%), Postives = 196/313 (62.62%), Query Frame = 0


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  -----VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPSCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP+ D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot
Match: Q9FX94 (Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 6.7e-75
Identity = 142/317 (44.79%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED WSA++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPS 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

[Close]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL
Match: A0A6J1G822 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111451728 PE=4 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 6.3e-193
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR
Sbjct: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE
Sbjct: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT
Sbjct: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP
Sbjct: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKCGC 323
           SCDSAADLVGQLAAFISGKCGC
Sbjct: 301 SCDSAADLVGQLAAFISGKCGC 322

[Close]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL
Match: A0A6J1L548 (probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111499977 PE=4 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 5.0e-190
Identity = 316/322 (98.14%), Postives = 321/322 (99.69%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR
Sbjct: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAAG+GKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVA+ANIVAVSVNYRRAPE
Sbjct: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFEDSW+ALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT
Sbjct: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSI+VVENLWYWVSKDIKDLDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP
Sbjct: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKCGC 323
           +CDSAADLVGQLAAFISGKC C
Sbjct: 301 TCDSAADLVGQLAAFISGKCRC 322

[Close]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL
Match: A0A6J1FSN2 (probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448111 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 276/320 (86.25%), Postives = 297/320 (92.81%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNA P+F++YKD RIDRLIGND DPPG+DPKTGVESKDVV+S EDSV+VRIYR
Sbjct: 1   MASDEIALNAVPYFVVYKDGRIDRLIGNDFDPPGVDPKTGVESKDVVVSPEDSVSVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAA   +  QKLPLLVYFHGGGFCIETAFSPFYHQH+N WVA+ANI AVSVNYRRAPE
Sbjct: 61  PKSAA---EQAQKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAKANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           +QLPIPFEDSW+A+KW+AAHS G GPDEWLNEIADLN+VYLAGDSAGGNIAHRMALR+AT
Sbjct: 121 YQLPIPFEDSWTAMKWVAAHSGGAGPDEWLNEIADLNRVYLAGDSAGGNIAHRMALRVAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPK I+VVENLWY VSKDIK+LDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKDIFVVENLWYVVSKDIKELDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDL RL AEKVA+YVAEKDNLKERGRHYAECLRKSGWGG V+VVETDGEGHVFHLFNP
Sbjct: 241 HDPDLARLPAEKVAVYVAEKDNLKERGRHYAECLRKSGWGGAVEVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKC 321
           +CDSAADLV  LAAF S  C
Sbjct: 301 TCDSAADLVELLAAFTSSDC 317

[Close]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL
Match: A0A5A7SP21 (Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold223G00540 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.0e-155
Identity = 260/320 (81.25%), Postives = 289/320 (90.31%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALNAAP+FI+YKD RIDRLIGNDIDPPGLDP TGVESKDV IS +  V VR+YR
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPD--VTVRVYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSA  A K  +KLPLLVYFHGGGFCIETAFSPFYHQH++ WVA+ANI+AVSVNYRRAPE
Sbjct: 61  PKSA--AEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+W+++KWIA+HS G G DEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGGKLIGEE +WDP  ++VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGRL AE+V +YVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRLPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKC 321
           +CD A +LV QLAAFI+  C
Sbjct: 301 TCDIAGELVKQLAAFINSGC 316

[Close]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL
Match: A0A1S3CA69 (probable carboxylesterase 12 OS=Cucumis melo OX=3656 GN=LOC103498399 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.8e-155
Identity = 259/320 (80.94%), Postives = 289/320 (90.31%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASD+IALNAAP+FI+YKD RIDRLIGNDIDPPGLDP TGVESKDV IS +  V VR+YR
Sbjct: 1   MASDDIALNAAPYFIVYKDGRIDRLIGNDIDPPGLDPITGVESKDVDISPD--VTVRVYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSA  A K  +KLPLLVYFHGGGFCIETAFSPFYHQH++ WVA+ANI+AVSVNYRRAPE
Sbjct: 61  PKSA--AEKISEKLPLLVYFHGGGFCIETAFSPFYHQHISAWVAEANIIAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFED+W+++KWIA+HS G G DEWLNEIADLNQVYLAGDSAGGN+AHRMALR  T
Sbjct: 121 HQLPIPFEDAWTSMKWIASHSEGKGQDEWLNEIADLNQVYLAGDSAGGNMAHRMALRTVT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGL GVKIKGLQLIHPHFWGGKLIGEE +WDP  ++VVENLW+ VSKDIK LDDPIVNPE
Sbjct: 181 EGLEGVKIKGLQLIHPHFWGGKLIGEENDWDPNDLFVVENLWFVVSKDIKTLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDLGR+ AE+V +YVAEKDNLKERGRHYAECL+KSGWGGTV+VVET+GEGHVFHLFNP
Sbjct: 241 HDPDLGRMPAERVGVYVAEKDNLKERGRHYAECLKKSGWGGTVEVVETEGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKC 321
           +CD A +LV QLAAFI+  C
Sbjct: 301 TCDIAGELVKQLAAFINSGC 316

[Close]

BLAST of CmoCh02G004540 vs. NCBI nr
Match: XP_022948027.1 (probable carboxylesterase 12 [Cucurbita moschata] >KAG6605132.1 putative carboxylesterase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 682.9 bits (1761), Expect = 1.3e-192
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR
Sbjct: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE
Sbjct: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT
Sbjct: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP
Sbjct: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKCGC 323
           SCDSAADLVGQLAAFISGKCGC
Sbjct: 301 SCDSAADLVGQLAAFISGKCGC 322

[Close]

BLAST of CmoCh02G004540 vs. NCBI nr
Match: XP_023534637.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676.0 bits (1743), Expect = 1.6e-190
Identity = 318/322 (98.76%), Postives = 320/322 (99.38%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR
Sbjct: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE
Sbjct: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFEDSW+ALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRM LRMAT
Sbjct: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMTLRMAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           HEP LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP
Sbjct: 241 HEPALGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKCGC 323
           +CDSAADLVGQLAAFISGKCGC
Sbjct: 301 TCDSAADLVGQLAAFISGKCGC 322

[Close]

BLAST of CmoCh02G004540 vs. NCBI nr
Match: XP_023007509.1 (probable carboxylesterase 12 [Cucurbita maxima])

HSP 1 Score: 673.3 bits (1736), Expect = 1.0e-189
Identity = 316/322 (98.14%), Postives = 321/322 (99.69%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR
Sbjct: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAAG+GKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVA+ANIVAVSVNYRRAPE
Sbjct: 61  PKSAAGSGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAEANIVAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           HQLPIPFEDSW+ALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT
Sbjct: 121 HQLPIPFEDSWTALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSI+VVENLWYWVSKDIKDLDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIHVVENLWYWVSKDIKDLDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP
Sbjct: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKCGC 323
           +CDSAADLVGQLAAFISGKC C
Sbjct: 301 TCDSAADLVGQLAAFISGKCRC 322

[Close]

BLAST of CmoCh02G004540 vs. NCBI nr
Match: XP_022943297.1 (probable carboxylesterase 12 [Cucurbita moschata] >KAG6571161.1 putative carboxylesterase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.4 bits (1526), Expect = 2.3e-165
Identity = 276/320 (86.25%), Postives = 297/320 (92.81%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNA P+F++YKD RIDRLIGND DPPG+DPKTGVESKDVV+S EDSV+VRIYR
Sbjct: 1   MASDEIALNAVPYFVVYKDGRIDRLIGNDFDPPGVDPKTGVESKDVVVSPEDSVSVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAA   +  QKLPLLVYFHGGGFCIETAFSPFYHQH+N WVA+ANI AVSVNYRRAPE
Sbjct: 61  PKSAA---EQAQKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAKANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           +QLPIPFEDSW+A+KW+AAHS G GPDEWLNEIADLN+VYLAGDSAGGNIAHRMALR+AT
Sbjct: 121 YQLPIPFEDSWTAMKWVAAHSGGAGPDEWLNEIADLNRVYLAGDSAGGNIAHRMALRVAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPK I+VVENLWY VSKDIK+LDDPIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKDIFVVENLWYVVSKDIKELDDPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDL RL AEKVA+YVAEKDNLKERGRHYAECLRKSGWGG V+VVETDGEGHVFHLFNP
Sbjct: 241 HDPDLARLPAEKVAVYVAEKDNLKERGRHYAECLRKSGWGGAVEVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKC 321
           +CDSAADLV  LAAF S  C
Sbjct: 301 TCDSAADLVELLAAFTSSDC 317

[Close]

BLAST of CmoCh02G004540 vs. NCBI nr
Match: XP_023511883.1 (probable carboxylesterase 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.6 bits (1516), Expect = 3.3e-164
Identity = 274/320 (85.62%), Postives = 296/320 (92.50%), Query Frame = 0


Query: 1   MASDEIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYR 60
           MASDEIALNA P+F++YKD RIDRLIGND DPPG+DPKTGVESKDVV+S EDSV+VRIYR
Sbjct: 1   MASDEIALNAVPYFVVYKDGRIDRLIGNDFDPPGVDPKTGVESKDVVVSPEDSVSVRIYR 60

Query: 61  PKSAAGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPE 120
           PKSAA   +  QKLPLLVYFHGGGFCIETAFSPFYHQH+N WVA+ANI AVSVNYRRAPE
Sbjct: 61  PKSAA---EQAQKLPLLVYFHGGGFCIETAFSPFYHQHINAWVAKANIAAVSVNYRRAPE 120

Query: 121 HQLPIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMAT 180
           +QLPIPFEDSW+A+KW+AAHS G GPDEWLNEIADLN+VYLAGDSAGGNIAHRMALR+AT
Sbjct: 121 YQLPIPFEDSWTAMKWVAAHSGGAGPDEWLNEIADLNRVYLAGDSAGGNIAHRMALRVAT 180

Query: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNPE 240
           EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPK I+VVENLWY VSKDIK+LD PIVNPE
Sbjct: 181 EGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKDIFVVENLWYVVSKDIKELDHPIVNPE 240

Query: 241 HEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNP 300
           H+PDL RL AEKVA+YVAEKDNLKERGRHYAECLRKSGWG  V+VVETDGEGHVFHLFNP
Sbjct: 241 HDPDLARLPAEKVAVYVAEKDNLKERGRHYAECLRKSGWGSAVEVVETDGEGHVFHLFNP 300

Query: 301 SCDSAADLVGQLAAFISGKC 321
           +CDSAA+LV QLAAF S  C
Sbjct: 301 TCDSAAELVEQLAAFTSSDC 317

[Close]

BLAST of CmoCh02G004540 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 8.6e-86
Identity = 159/321 (49.53%), Postives = 209/321 (65.11%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA++ +P   IYK  RI+RL+G    PP  +P+ GV SKDVV S +++++VRIY P+ A
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
             A + D KLPLLVYFHGGGF IETAFSP YH  L   V+ +N VAVSV+YRRAPEH + 
Sbjct: 64  --AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 123

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATE--- 184
           +PF+DSW+ALKW+  H  G+G ++WLN+ AD ++V+L+GDSAG NI H MA+R A E   
Sbjct: 124 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 183

Query: 185 -GLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIVN- 244
            GL    I G+ L+HP+FW    I E+   D      +E  W   S + KD  DDP++N 
Sbjct: 184 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 243

Query: 245 -PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHL 304
                 DL  L   KV + VAEKD L  +G  YA  L KSGW G V+VVE++GE HVFHL
Sbjct: 244 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 303

Query: 305 FNPSCDSAADLVGQLAAFISG 319
             P CD+A +++ + + FI G
Sbjct: 304 LKPECDNAIEVMHKFSGFIKG 322

[Close]

BLAST of CmoCh02G004540 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 295.8 bits (756), Expect = 4.2e-80
Identity = 149/315 (47.30%), Postives = 204/315 (64.76%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           +++L   PW +++ D  ++RL G ++ PPGLDP TGV SKD++I  +  ++ RIYRP S 
Sbjct: 7   QVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIYRPFSI 66

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
               +  QK+PL++YFHGG F I +   P YH  LN  V QAN++AVSVNYR APEH LP
Sbjct: 67  ----QPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLP 126

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +EDSW+ALK I A +     + W+N+ ADL+ ++L GDSAG NI+H +A R A +   
Sbjct: 127 TAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFR-AKQSDQ 186

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKDLDDPIVNP--EHE 244
            +KIKG+ +IHP+FWG + IG E + D     +V+  W +V    K  DDP +NP  +  
Sbjct: 187 TLKIKGIGMIHPYFWGTQPIGAEIK-DEARKQMVDGWWEFVCPSEKGSDDPWINPFADGS 246

Query: 245 PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPSC 304
           PDLG L  E+V I VAEKD L ERG+ Y E L KS W G V+++ET  + HVFH+F P C
Sbjct: 247 PDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEPDC 306

Query: 305 DSAADLVGQLAAFIS 318
           D A ++V  LA FI+
Sbjct: 307 DEAMEMVRCLALFIN 310

[Close]

BLAST of CmoCh02G004540 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 295.4 bits (755), Expect = 5.4e-80
Identity = 154/324 (47.53%), Postives = 209/324 (64.51%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA + +P  IIYK  RI+RL+G    PP  +P+ GV SKDVV S ++++++RIY P+ A
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 65  AGA-GKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQL 124
           A A  +   KLPLLVYFHGGGF +ETAFSP YH  L   V+ ++ VAVSV+YRRAPEH +
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 125 PIPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMAL-----RM 184
           P  ++DSW+ALKW+ +H  G+G ++WLN+ AD ++V+LAGDSAG NI H M +     ++
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 185 ATEGLAGVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIV 244
           + E L    I G+ L+HP+FW    + +++  D      +E++W   S + KD  DDP +
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 245 N--PEHEPDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTV-DVVETDGEGHV 304
           N       DL  L   KV + VAEKD L  +G  Y E L KS W G V DVVET GEGHV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

Query: 305 FHLFNPSCDSAADLVGQLAAFISG 319
           FHL +P+ + A +LV + A FI G
Sbjct: 304 FHLRDPNSEKAHELVHRFAGFIKG 327

[Close]

BLAST of CmoCh02G004540 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 6.0e-79
Identity = 149/313 (47.60%), Postives = 196/313 (62.62%), Query Frame = 0


Query: 6   IALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSAA 65
           IA + +P F +YK  RI+RL+G    PP L P+ GV SKD++ S E ++++RIY P+   
Sbjct: 5   IAFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKVT 64

Query: 66  GAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLPI 125
                 +KLP+L+YFHGGGF IETAFSP YH  L   VA AN +A+SVNYRRAPE  +PI
Sbjct: 65  -----VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPI 124

Query: 126 PFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLAG 185
           P+EDSW +LKW+  H  GTGP+ W+N+  D  +V+LAGDSAGGNI+H + +R   E L  
Sbjct: 125 PYEDSWDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCD 184

Query: 186 VKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVSKDIKD-LDDPIVNPEHEPD 245
             I G+ LIHP+FW    I E +  D      VE  W   S + K  +DDP +N      
Sbjct: 185 SLISGIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP 244

Query: 246 LGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPSCDS 305
            G L   +V + VA  D    +G  YAE L+KSGW G V+V+ET  EGHVFHL NP+ D+
Sbjct: 245 SG-LGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDN 304

Query: 306 AADLVGQLAAFIS 318
           A  +V +L  FI+
Sbjct: 305 ARQVVKKLEEFIN 311

[Close]

BLAST of CmoCh02G004540 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 282.3 bits (721), Expect = 4.8e-76
Identity = 142/317 (44.79%), Postives = 192/317 (60.57%), Query Frame = 0


Query: 5   EIALNAAPWFIIYKDRRIDRLIGNDIDPPGLDPKTGVESKDVVISQEDSVAVRIYRPKSA 64
           EIA    P+  IYKD R++RLIG D  P  LDP   V SKDV+ S E++++VR++ P  +
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 65  AGAGKGDQKLPLLVYFHGGGFCIETAFSPFYHQHLNPWVAQANIVAVSVNYRRAPEHQLP 124
                G+ KLPLL+Y HGG + IE+ FSP YH +L   V  AN +AVSV YRRAPE  +P
Sbjct: 64  TKLTAGN-KLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVP 123

Query: 125 IPFEDSWSALKWIAAHSNGTGPDEWLNEIADLNQVYLAGDSAGGNIAHRMALRMATEGLA 184
             +ED WSA++WI AHSNG+GP +W+N+ AD  +V+L GDSAGGNI+H MA++   E   
Sbjct: 124 AAYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKKL 183

Query: 185 GVKIKGLQLIHPHFWGGKLIGEEKEWDPKSIYVVENLWYWVS--KDIKDLDDPIVNPEHE 244
            +KIKG+ ++HP FWG   + E    D ++   +  +W  ++    +   DDP+ N    
Sbjct: 184 DLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFNVNGS 243

Query: 245 -PDLGRLAAEKVAIYVAEKDNLKERGRHYAECLRKSGWGGTVDVVETDGEGHVFHLFNPS 304
             D   L  +KV + VA KD    +G  YA  L K  W GTV+VVE +GE HVFHL NP 
Sbjct: 244 GSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQNPK 303

Query: 305 CDSAADLVGQLAAFISG 319
            D A   + +   FI G
Sbjct: 304 SDKALKFLKKFVEFIIG 319

The following BLAST results are available for this feature:

BLAST of CmoCh02G004540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SMN0	1.2e-84	49.53	Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1	[more]
Q9SX78	5.8e-79	47.30	Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1	[more]
Q9SMM9	7.6e-79	47.53	Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1	[more]
Q9ZQ91	8.4e-78	47.60	Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1	[more]
Q9FX94	6.7e-75	44.79	Probable carboxylesterase 5 OS=Arabidopsis thaliana OX=3702 GN=CXE5 PE=2 SV=1	[more]

BLAST of CmoCh02G004540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G822	6.3e-193	100.00	probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111451728 PE=4 ...	[more]
A0A6J1L548	5.0e-190	98.14	probable carboxylesterase 12 OS=Cucurbita maxima OX=3661 GN=LOC111499977 PE=4 SV...	[more]
A0A6J1FSN2	1.1e-165	86.25	probable carboxylesterase 12 OS=Cucurbita moschata OX=3662 GN=LOC111448111 PE=4 ...	[more]
A0A5A7SP21	1.0e-155	81.25	Putative carboxylesterase 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3CA69	1.8e-155	80.94	probable carboxylesterase 12 OS=Cucumis melo OX=3656 GN=LOC103498399 PE=4 SV=1	[more]

BLAST of CmoCh02G004540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948027.1	1.3e-192	100.00	probable carboxylesterase 12 [Cucurbita moschata] >KAG6605132.1 putative carboxy...	[more]
XP_023534637.1	1.6e-190	98.76	probable carboxylesterase 12 [Cucurbita pepo subsp. pepo]	[more]
XP_023007509.1	1.0e-189	98.14	probable carboxylesterase 12 [Cucurbita maxima]	[more]
XP_022943297.1	2.3e-165	86.25	probable carboxylesterase 12 [Cucurbita moschata] >KAG6571161.1 putative carboxy...	[more]
XP_023511883.1	3.3e-164	85.63	probable carboxylesterase 12 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmoCh02G004540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48690.1	8.6e-86	49.53	alpha/beta-Hydrolases superfamily protein	[more]
AT1G47480.1	4.2e-80	47.30	alpha/beta-Hydrolases superfamily protein	[more]
AT3G48700.1	5.4e-80	47.53	carboxyesterase 13	[more]
AT2G03550.1	6.0e-79	47.60	alpha/beta-Hydrolases superfamily protein	[more]
AT1G49660.1	4.8e-76	44.79	carboxyesterase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 16..319 e-value: 7.0E-69 score: 234.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 8..317
IPR013094	Alpha/beta hydrolase fold-3	PFAM	PF07859	Abhydrolase_3	coord: 77..296 e-value: 1.4E-46 score: 159.1
None	No IPR available	PANTHER	PTHR23024	ARYLACETAMIDE DEACETYLASE	coord: 3..317
None	No IPR available	PANTHER	PTHR23024:SF458	CARBOXYLESTERASE 2-RELATED	coord: 3..317
IPR033140	Lipase, GDXG, putative serine active site	PROSITE	PS01174	LIPASE_GDXG_SER	coord: 159..171

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G004540.1	CmoCh02G004540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity