CmoCh02G003260 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G003260
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptiontriosephosphate isomerase
LocationCmo_Chr02: 1682817 .. 1684385 (+)
RNA-Seq ExpressionCmoCh02G003260
SyntenyCmoCh02G003260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCAAGCATTCCATGAGGACAGTCGAAGTGTGCAAGTTGGCTTCGATACTTACGGGTATATATACACACACATATAAAATAAGAGTTTGAGAAATTCAAGTCCCTAAGAGACTTTGATGTTTATAAGTTACCCTTGCTTAAGCCGATAATTTTATTTAATTTAAATTGTTAGCTTTCCTTTTTGAGCTTTTTTACCTTTTACGGGGAAGGCTTTCGAAGAGCAATTGTGCATGATTGAGCTACTTTCTGTTTAAGATCAGATGCCTGTTAAACTTGTCTTTTCCTTTGTAACTCAATACTGCGGATTTTTCAAAAAAAATGTCTCATGTGTGTTTTGAGTTTTCCGCTTACTAATATATGTTAGATCTTTGCATTATTTAAATTAATTTGTATGTGCTGCAGTGTTGAACAATTGAAAGATCTAGGCTGCAAGTGGGTTATCCTTGGGCACTCGGAACGTAGACATATCATTGGTGAAGATGACCAGGTGAATGTTATGAAATTGAGTGGTTCCCAAGACATCAGTGTTCAAGCTGATTCTCTCCAACGCTGACTGATGCTTTGGCAACTGTAGTTCATAGGAAAGAAGGCTGCCTATGCCTTGAGCGAGGGTCTTGGAGTAATGGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTCGATGTTTGCTTTCGGCAATTGAAAGCTTTTGCAGGCAAGTGTTACTAGCTCACTTATTCCTTCACATCGATGATCTATAGTCACAGATCTGAGAACAGAAAAGTGCTGGAGTTCTCTGTATAACTTTAGTATTTCCCATTCACTGCAGATGCTGTTCCGAGTTGGGATGGTATTGTTATTGCTTATGAGCCAGTATGGGCCATTGGGACTGGCAAAGTGGCTTCGCCCGAGCAAGCTCAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATGTATCACCAGAAGTGGCATCTAAAACACGAATTATTTATGGAGGTAACTACCGCCTCCATTGCACTACTGTTAACTTATAGCTGCTGTAGAAATAAATTTACTTATCTGAATCTTGAGCGGCTCATATGTCTAAGTATCGGTATTATTGTCAGGATCGGTGAATGGAAGTAATTGTGCGGAGCTTGCCAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGTGCATTTCTAACATCTCCTATCAAATTATAAGAATGAAATTTAGAAGATGTCACTTGAAGTTCCCATCTCAACTTCTGTTCGATGTTGCGATCGCAGGGTCCCGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGAAATCTTTTAGGAGTTCCTGGTGTCAAGCACGAGAGGTAAGGGTGGCAATGTTTTGTATCCATAGCAACACCCCTTGGGGTTGTCAAGTTTTCAGCGTGTTCATGTTCTTGCTGTGAATGCTAGAAAATATTATCTTTTTGTTGTATTCCTGAGGTTACTTTGCTATAGAATAATTTGAATACCTTAATAAGAGTTCTTGAATCTATCTCGGTTATTTTGAAC

mRNA sequence

ATGGACCAAGCATTCCATGAGGACAGTCGAAGTGTGCAAGTTGGCTTCGATACTTACGGTGTTGAACAATTGAAAGATCTAGGCTGCAAGTGGGTTATCCTTGGGCACTCGGAACGTAGACATATCATTGGTGAAGATGACCAGTTCATAGGAAAGAAGGCTGCCTATGCCTTGAGCGAGGGTCTTGGAGTAATGGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTCGATGTTTGCTTTCGGCAATTGAAAGCTTTTGCAGGCAAGTATGCTGTTCCGAGTTGGGATGGTATTGTTATTGCTTATGAGCCAGTATGGGCCATTGGGACTGGCAAAGTGGCTTCGCCCGAGCAAGCTCAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATGTATCACCAGAAGTGGCATCTAAAACACGAATTATTTATGGAGGATCGGTGAATGGAAGTAATTGTGCGGAGCTTGCCAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGTCCCGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGAAATCTTTTAGGAGTTCCTGGTGTCAAGCACGAGAGGTAAGGGTGGCAATGTTTTGTATCCATAGCAACACCCCTTGGGGTTGTCAAGTTTTCAGCGTGTTCATGTTCTTGCTGTGAATGCTAGAAAATATTATCTTTTTGTTGTATTCCTGAGGTTACTTTGCTATAGAATAATTTGAATACCTTAATAAGAGTTCTTGAATCTATCTCGGTTATTTTGAAC

Coding sequence (CDS)

ATGGACCAAGCATTCCATGAGGACAGTCGAAGTGTGCAAGTTGGCTTCGATACTTACGGTGTTGAACAATTGAAAGATCTAGGCTGCAAGTGGGTTATCCTTGGGCACTCGGAACGTAGACATATCATTGGTGAAGATGACCAGTTCATAGGAAAGAAGGCTGCCTATGCCTTGAGCGAGGGTCTTGGAGTAATGGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTCGATGTTTGCTTTCGGCAATTGAAAGCTTTTGCAGGCAAGTATGCTGTTCCGAGTTGGGATGGTATTGTTATTGCTTATGAGCCAGTATGGGCCATTGGGACTGGCAAAGTGGCTTCGCCCGAGCAAGCTCAGGAAGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAATGTATCACCAGAAGTGGCATCTAAAACACGAATTATTTATGGAGGATCGGTGAATGGAAGTAATTGTGCGGAGCTTGCCAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGTCCCGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGA

Protein sequence

MDQAFHEDSRSVQVGFDTYGVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKTFDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Homology
BLAST of CmoCh02G003260 vs. ExPASy Swiss-Prot
Match: P48496 (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 1.8e-86
Identity = 154/178 (86.52%), Postives = 169/178 (94.94%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGC+WVILGHSERRH+IGE ++FIGKKAAYAL++G+GV+ACIGELLEEREAGKT
Sbjct: 147 VEQLKDLGCQWVILGHSERRHVIGEQNEFIGKKAAYALNQGVGVIACIGELLEEREAGKT 206

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVC++QLKAFA   A+PSWD +V+AYEPVWAIGTGKVASP+QAQEVH AVRDWLKKNVS
Sbjct: 207 FDVCYQQLKAFAD--ALPSWDNVVVAYEPVWAIGTGKVASPDQAQEVHVAVRDWLKKNVS 266

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
            EVASKTRIIYGGSVNG NCAELAK+EDIDGFLVGGASLKGPEF TIVNSVTAKKVAA
Sbjct: 267 EEVASKTRIIYGGSVNGGNCAELAKQEDIDGFLVGGASLKGPEFATIVNSVTAKKVAA 322

BLAST of CmoCh02G003260 vs. ExPASy Swiss-Prot
Match: Q9SKP6 (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 5.2e-86
Identity = 158/178 (88.76%), Postives = 165/178 (92.70%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRH+IGE D+FIGKKAAYALSEGLGV+ACIGE LEEREAGKT
Sbjct: 140 VEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKT 199

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCF QLKAFA   AVPSWD IV+AYEPVWAIGTGKVASP+QAQEVH AVR WLKKNVS
Sbjct: 200 FDVCFAQLKAFAD--AVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWLKKNVS 259

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
            EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPEF TIVNSVT+KKVAA
Sbjct: 260 EEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTSKKVAA 315

BLAST of CmoCh02G003260 vs. ExPASy Swiss-Prot
Match: Q9M4S8 (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 1.7e-84
Identity = 154/178 (86.52%), Postives = 166/178 (93.26%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKD+G KWVILGHSERRH+IGEDDQFIGKKAAYAL+EGLGV+ACIGE LEEREAGKT
Sbjct: 139 VEQLKDIGRKWVILGHSERRHVIGEDDQFIGKKAAYALNEGLGVIACIGEKLEEREAGKT 198

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVC+ QLKAFA   AVPSW+ IV+AYEPVWAIGTGKVASP+QAQEVH AVR+WLKKNVS
Sbjct: 199 FDVCYEQLKAFAD--AVPSWENIVVAYEPVWAIGTGKVASPQQAQEVHVAVREWLKKNVS 258

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
            EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPEF TIVN+VT+KKVAA
Sbjct: 259 AEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNAVTSKKVAA 314

BLAST of CmoCh02G003260 vs. ExPASy Swiss-Prot
Match: P46225 (Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 2.5e-80
Identity = 145/177 (81.92%), Postives = 158/177 (89.27%), Query Frame = 0

Query: 22  EQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKTF 81
           EQL D+GC+WVILGHSERRH+IGEDD+FIGKKAAYALS+ L VMACIGELLEEREAGKTF
Sbjct: 124 EQLVDIGCQWVILGHSERRHVIGEDDEFIGKKAAYALSQNLKVMACIGELLEEREAGKTF 183

Query: 82  DVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVSP 141
           DVCF+Q+KAFA    +  W  +VIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLK NVS 
Sbjct: 184 DVCFKQMKAFADN--ITDWTNVVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKTNVSA 243

Query: 142 EVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           +VAS  RIIYGGSVN +NCAELAKKEDIDGFLVGGASLKGP+F TI NSVT+KKV A
Sbjct: 244 DVASTVRIIYGGSVNAANCAELAKKEDIDGFLVGGASLKGPDFATICNSVTSKKVTA 298

BLAST of CmoCh02G003260 vs. ExPASy Swiss-Prot
Match: P48497 (Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 4.9e-76
Identity = 138/177 (77.97%), Postives = 155/177 (87.57%), Query Frame = 0

Query: 22  EQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKTF 81
           + L +LG  WVILGHSERR ++GE ++F+GKKAAYA SEGLGV+ACIGELLEEREAGKTF
Sbjct: 82  QMLANLGITWVILGHSERRTLLGESNEFVGKKAAYAQSEGLGVIACIGELLEEREAGKTF 141

Query: 82  DVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVSP 141
           DVCF+QLK+FA ++    WD +V+AYEPVWAIGTGKVASPEQAQEVH AVRDWLK NVS 
Sbjct: 142 DVCFKQLKSFA-RFPAKPWDNVVVAYEPVWAIGTGKVASPEQAQEVHVAVRDWLKTNVSE 201

Query: 142 EVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           EVASKTRIIYGGSVNG N   LA +ED+DGFLVGGASLKGPEF TI+NSVTAKKVAA
Sbjct: 202 EVASKTRIIYGGSVNGGNSLALAAQEDVDGFLVGGASLKGPEFATIINSVTAKKVAA 257

BLAST of CmoCh02G003260 vs. ExPASy TrEMBL
Match: A0A6J1G6X6 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451334 PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.5e-93
Identity = 175/178 (98.31%), Postives = 175/178 (98.31%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. ExPASy TrEMBL
Match: A0A6J1L7Y2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111500005 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.9e-91
Identity = 172/178 (96.63%), Postives = 174/178 (97.75%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKA+YALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKASYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWD IVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDDIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. ExPASy TrEMBL
Match: A0A6J1JFM2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111484585 PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 2.8e-90
Identity = 167/178 (93.82%), Postives = 174/178 (97.75%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKD+GCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCF+QLKA+A   AVPSWDGIVIAYEPVWAIGTGKVA+P+QAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFQQLKAYAD--AVPSWDGIVIAYEPVWAIGTGKVATPDQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. ExPASy TrEMBL
Match: A0A6J1CXY1 (triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015469 PE=3 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 3.6e-90
Identity = 166/178 (93.26%), Postives = 173/178 (97.19%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCF+QLKAFA   A+PSWDG+VIAYEPVWAIGTGKVA+PEQAQEVHAAVRDWLKKN+S
Sbjct: 191 FDVCFQQLKAFAD--AIPSWDGVVIAYEPVWAIGTGKVATPEQAQEVHAAVRDWLKKNIS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSV AKKV A
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVAAKKVVA 306

BLAST of CmoCh02G003260 vs. ExPASy TrEMBL
Match: A0A5A7TRC4 (Triosephosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G002060 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.7e-90
Identity = 167/179 (93.30%), Postives = 174/179 (97.21%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKD+GCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKY-AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNV 140
           FDVCF+QLKA+AG   AVPSWD IVIAYEPVWAIGTGKVA+PEQAQEVHAA+RDWLKKNV
Sbjct: 191 FDVCFQQLKAYAGNADAVPSWDSIVIAYEPVWAIGTGKVATPEQAQEVHAAIRDWLKKNV 250

Query: 141 SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           S EVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 SSEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 309

BLAST of CmoCh02G003260 vs. NCBI nr
Match: KAG7035038.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.8 bits (930), Expect = 1.8e-96
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 308

BLAST of CmoCh02G003260 vs. NCBI nr
Match: XP_022947479.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 9.4e-93
Identity = 175/178 (98.31%), Postives = 175/178 (98.31%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. NCBI nr
Match: XP_023533234.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.5 bits (898), Expect = 9.4e-93
Identity = 175/178 (98.31%), Postives = 175/178 (98.31%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. NCBI nr
Match: KAG6605006.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 349.0 bits (894), Expect = 2.7e-92
Identity = 174/178 (97.75%), Postives = 175/178 (98.31%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. NCBI nr
Match: XP_023007543.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 345.1 bits (884), Expect = 3.9e-91
Identity = 172/178 (96.63%), Postives = 174/178 (97.75%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKA+YALSEGLGV+ACIGELLEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKASYALSEGLGVIACIGELLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCFRQLKAFA   AVPSWD IVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS
Sbjct: 191 FDVCFRQLKAFAD--AVPSWDDIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
           PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Sbjct: 251 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 306

BLAST of CmoCh02G003260 vs. TAIR 10
Match: AT2G21170.1 (triosephosphate isomerase )

HSP 1 Score: 318.5 bits (815), Expect = 3.7e-87
Identity = 158/178 (88.76%), Postives = 165/178 (92.70%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRH+IGE D+FIGKKAAYALSEGLGV+ACIGE LEEREAGKT
Sbjct: 140 VEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKT 199

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCF QLKAFA   AVPSWD IV+AYEPVWAIGTGKVASP+QAQEVH AVR WLKKNVS
Sbjct: 200 FDVCFAQLKAFAD--AVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWLKKNVS 259

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
            EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPEF TIVNSVT+KKVAA
Sbjct: 260 EEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTSKKVAA 315

BLAST of CmoCh02G003260 vs. TAIR 10
Match: AT2G21170.2 (triosephosphate isomerase )

HSP 1 Score: 318.5 bits (815), Expect = 3.7e-87
Identity = 158/178 (88.76%), Postives = 165/178 (92.70%), Query Frame = 0

Query: 21  VEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKT 80
           VEQLKDLGCKWVILGHSERRH+IGE D+FIGKKAAYALSEGLGV+ACIGE LEEREAGKT
Sbjct: 131 VEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEGLGVIACIGEKLEEREAGKT 190

Query: 81  FDVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVS 140
           FDVCF QLKAFA   AVPSWD IV+AYEPVWAIGTGKVASP+QAQEVH AVR WLKKNVS
Sbjct: 191 FDVCFAQLKAFAD--AVPSWDNIVVAYEPVWAIGTGKVASPQQAQEVHVAVRGWLKKNVS 250

Query: 141 PEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA 199
            EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPEF TIVNSVT+KKVAA
Sbjct: 251 EEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPEFATIVNSVTSKKVAA 306

BLAST of CmoCh02G003260 vs. TAIR 10
Match: AT3G55440.1 (triosephosphate isomerase )

HSP 1 Score: 221.5 bits (563), Expect = 6.1e-58
Identity = 112/176 (63.64%), Postives = 130/176 (73.86%), Query Frame = 0

Query: 22  EQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGELLEEREAGKTF 81
           E L +L   WVILGHSERR I+ E  +F+G K AYAL++GL V+AC+GE LEEREAG T 
Sbjct: 82  EMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQGLKVIACVGETLEEREAGSTM 141

Query: 82  DVCFRQLKAFAGKYAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVRDWLKKNVSP 141
           DV   Q KA A +  V +W  +VIAYEPVWAIGTGKVASP QAQEVH  +R WL KNVS 
Sbjct: 142 DVVAAQTKAIADR--VTNWSNVVIAYEPVWAIGTGKVASPAQAQEVHDELRKWLAKNVSA 201

Query: 142 EVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVA 198
           +VA+ TRIIYGGSVNG NC EL  + D+DGFLVGGASLK PEF  I+ +   KK A
Sbjct: 202 DVAATTRIIYGGSVNGGNCKELGGQADVDGFLVGGASLK-PEFIDIIKAAEVKKSA 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P484961.8e-8686.52Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 P... [more]
Q9SKP65.2e-8688.76Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM ... [more]
Q9M4S81.7e-8486.52Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=... [more]
P462252.5e-8081.92Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1[more]
P484974.9e-7677.97Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1G6X64.5e-9398.31triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1L7Y21.9e-9196.63triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1JFM22.8e-9093.82triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1CXY13.6e-9093.26triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A5A7TRC44.7e-9093.30Triosephosphate isomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
Match NameE-valueIdentityDescription
KAG7035038.11.8e-96100.00Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_022947479.19.4e-9398.31triosephosphate isomerase, chloroplastic-like [Cucurbita moschata][more]
XP_023533234.19.4e-9398.31triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6605006.12.7e-9297.75Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_023007543.13.9e-9196.63triosephosphate isomerase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G21170.13.7e-8788.76triosephosphate isomerase [more]
AT2G21170.23.7e-8788.76triosephosphate isomerase [more]
AT3G55440.16.1e-5863.64triosephosphate isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000652Triosephosphate isomerasePFAMPF00121TIMcoord: 21..188
e-value: 2.8E-66
score: 223.4
IPR000652Triosephosphate isomeraseTIGRFAMTIGR00419TIGR00419coord: 22..180
e-value: 2.3E-55
score: 185.9
IPR000652Triosephosphate isomerasePANTHERPTHR21139TRIOSEPHOSPHATE ISOMERASEcoord: 20..196
IPR000652Triosephosphate isomerasePROSITEPS51440TIM_2coord: 1..190
score: 58.99511
IPR000652Triosephosphate isomeraseCDDcd00311TIMcoord: 21..189
e-value: 2.78879E-93
score: 270.176
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 17..197
e-value: 1.9E-81
score: 275.1
NoneNo IPR availablePANTHERPTHR21139:SF20TRIOSEPHOSPHATE ISOMERASE, CHLOROPLASTICcoord: 20..196
IPR020861Triosephosphate isomerase, active sitePROSITEPS00171TIM_1coord: 106..116
IPR035990Triosephosphate isomerase superfamilySUPERFAMILY51351Triosephosphate isomerase (TIM)coord: 21..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G003260.1CmoCh02G003260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006094 gluconeogenesis
biological_process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0006096 glycolytic process
biological_process GO:0032504 multicellular organism reproduction
biological_process GO:0080022 primary root development
biological_process GO:0006642 triglyceride mobilization
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
molecular_function GO:0004807 triose-phosphate isomerase activity
molecular_function GO:0003824 catalytic activity