CmoCh02G003250 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G003250
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTriosephosphate isomerase
LocationCmo_Chr02: 1679683 .. 1682437 (+)
RNA-Seq ExpressionCmoCh02G003250
SyntenyCmoCh02G003250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTGGTGTCTACTTCTCTTGCATCTCAACTCTCAGCTGTCCGATTGTCAGGTTCCAACTCCGACATTTCTCAGACTCTATTGCAAAACGTTCGCTCACAAATCCGCCTTGCATCCTCAAGGAAAGGCTCCCGTGGCGTTGTTACCATGGCCGGCTCCGGAAAGGTATTGAACCTTTTTTCATTAGTTTATTCGCGTTCGGCGCTTGAATCGTTTCGGCTCCCTGAAGAATTTCAGATTCGTGTAAGAGGACGAGAAGCTACGAGGAAACTTTTTGCTTATTTCTAATTTCTAATTATTATCATTCTTTGTTGTATTTGCAGTTCTTCGTTGGTGGAAACTGGAAATGCGTAAGTATCTTCATTATCGATAATCTTATTGCTAAAGATCTGTGGTGGATGGTTATATGTCAAGCAAAAAAATTCTTATGTACGGCATACGTGCATCCTATGATTTTTCTTCAAATCATTCTATTTTTCTGTTCTGTCCACAATCTCTCGAGCATCCGTATAAAACATAAGTCTTTTCACCATGTTTCTAGAACTTTGCTTTTCGGTAACAACTCGCGATGAGAATGTGGTTGATTTTCATTTATTTATCAACTATTAATTGGTTTAGTTGGATTTGTATGAACCTCTTGTTAGCTACTAAAGGCAAATATGTACAAATCATTTGAAATAGATAAAGCATTTAGATAAATAGACCACATTCTTTTGTCTGTATTGTTAGTTGGACACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCTTTGTTTTGGGCTTCCCCAAAAGGCCTCATACCAATGGAGAGAGTATTCTTTGATTATAAACCCATGATCATTTCTTAAATTAGTCAACGTGGGACTTTCATCATCCAATATGTATAACTTCATTTTCATGGGCAAAATGCTTTAAGTTTGAAACTGGTTAGGCTCAAGCTATCCCTTCTTAGATCATACAAAGGTTCTTCTGCCACTTCTGAATTATAATGATTGAAATCTTCATAAACTTTCCTGTCTTCTAATACTTGATCATTAGGAACCCTTTGTTGTTTACGGTTTGTAAGTTTTTAAAATGTACTGGATCCATTATAGTACCCAAAATAAAGTCAGTTTTCTTCCTGCTACTAATTATTTCTGATTTGTGATCGACTTATATTGATTCTTTCCTCTTTATTCTAATTTCCAGAATGGTACAAAGGAATCTATATCCAAGCTTGTCGCAGACTTGAACAATGCAAAGTTGGAAGATGACGTTGGTAAGTTAAGTCTTATATTGGAGGATGCCTGTTATCACCGTTTCATTATATGTTCTTGTTATAAAAAATAAAAAGGTCAGGTTTGAGGTTGCAATTTTTCCCTCTCATATTCACTTGGCAATCAATTACGCAATTCCTAAAAAACAAAGGTGGCGTTTGATACCGGCCTGTTCCTAACTTTATTACATCCCCTCCTTTTTTAAGAACACTCATATCAAGACATCTATCAATTTGATAGTGGGTTAGCATTAGACTTTTGATATAATTAATATTGTATTAGAGTAGAAGGTTCTTTGCTCATCCTGTGTTTGAACTCCCAAAATGTAACGACCCGACTTCTCGCATACGTACCGGTCGGAAATGTCACTTAACACTAAAAGTTTATAGTTTAAAATACATGAATAGATGCTAAAAGACACTTTAGTGAAATCATGCTTGGGTACACCTTTCTAAAGAAATTTTGCAAGGCATCCAATTTAACCAAATAGTTCCTCGGTGAGCATACATATCTAACCGGATTTAGAAAATAAAACGGTTTGAGTTTTAATAGAAAGGTCTATTATCTAGAGATTTCGCAATAACAATTTAGACATGTGGGTCCTATTCCCACTTTCCAAAAAGAATTAAGCTTGGTTGGTTAGCATGTCGAACCTCCAACTTTAGTATTCCGGTTACTATATTTACATTTATAGGTGACTATTAGTGGTATGTTGCATCCAAAATCAAGATGGGAGTAAAATAACAAGCCCCACAAAACTAACTGTTTTCTCCTCCAAACGATGGTTATGGCGGCAATGGTGCAGTGTGATGGCAATATGAGCGACCATACATGGCAGGGCAGTTGGTAGCTGTCATGACGGGTGTGGGCGGCAAAGAGAAGGACAAAAATAGGAGAAGGGAGGCGATGGTGTGTATAAGGAAGGAGAAGAAGAGGAGACGGTGGCGAGGTTTTGATCTGCATACTACAGAGAGAGGAGGTGGCTAGGGTTTTGTTTTTTCGTTTTTTTTTTCCCCTTGGCTAGGCTTTTACATAAATTGTCATTTTCTCCCCTATTAATATTATGTTTCACTGGTTGGCTCTTCCTACAAAATTTCAAGCCCACAAGTGTTGATATAATTGAATTTACCATAACTCATCAACTTAAGTTTTTGGTTTGATTGGTGATTTAATATTTACATTAATTTATTAATACATCTGAATGGTTGATGAGGATGAATGACATTTTTTGTATTCTTTTTTTTTCCACATTTTTTATTATACTCTTCAAGTACATTCTTCTGAAAAAAGATATTTGGATGTGGGCGGAGGAATGCAGATGTTGTTGTAGCACCTCCCTTTGTTTATATTGATCAGGTGAAGAGCTCGTTGGCATCAAGGATTGAGATTTCTGCCCAAAATTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGGTTAATCTTGCTTGCCCTTGATGAGACATTTTAA

mRNA sequence

ATGGCGGTGGTGTCTACTTCTCTTGCATCTCAACTCTCAGCTGTCCGATTGTCAGGTTCCAACTCCGACATTTCTCAGACTCTATTGCAAAACGTTCGCTCACAAATCCGCCTTGCATCCTCAAGGAAAGGCTCCCGTGGCGTTGTTACCATGGCCGGCTCCGGAAAGGTATTGAACCTTTTTTCATTAGTTTATTCGCGTTCGGCGCTTGAATCGTTTCGGCTCCCTGAAGAATTTCAGATTCGTTTCTTCGTTGGTGGAAACTGGAAATGCGTAAGTATCTTCATTATCGATAATCTTATTGCTAAAGATCTGTGGTGGATGAATGGTACAAAGGAATCTATATCCAAGCTTGTCGCAGACTTGAACAATGCAAAGTTGGAAGATGACGTTGATGTTGTTGTAGCACCTCCCTTTGTTTATATTGATCAGGTGAAGAGCTCGTTGGCATCAAGGATTGAGATTTCTGCCCAAAATTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGGTTAATCTTGCTTGCCCTTGATGAGACATTTTAA

Coding sequence (CDS)

ATGGCGGTGGTGTCTACTTCTCTTGCATCTCAACTCTCAGCTGTCCGATTGTCAGGTTCCAACTCCGACATTTCTCAGACTCTATTGCAAAACGTTCGCTCACAAATCCGCCTTGCATCCTCAAGGAAAGGCTCCCGTGGCGTTGTTACCATGGCCGGCTCCGGAAAGGTATTGAACCTTTTTTCATTAGTTTATTCGCGTTCGGCGCTTGAATCGTTTCGGCTCCCTGAAGAATTTCAGATTCGTTTCTTCGTTGGTGGAAACTGGAAATGCGTAAGTATCTTCATTATCGATAATCTTATTGCTAAAGATCTGTGGTGGATGAATGGTACAAAGGAATCTATATCCAAGCTTGTCGCAGACTTGAACAATGCAAAGTTGGAAGATGACGTTGATGTTGTTGTAGCACCTCCCTTTGTTTATATTGATCAGGTGAAGAGCTCGTTGGCATCAAGGATTGAGATTTCTGCCCAAAATTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGGTTAATCTTGCTTGCCCTTGATGAGACATTTTAA

Protein sequence

MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEIRLILLALDETF
Homology
BLAST of CmoCh02G003250 vs. ExPASy Swiss-Prot
Match: P48496 (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 8.0e-33
Identity = 97/188 (51.60%), Postives = 111/188 (59.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLS------AVRLSG--------SNSDISQ-TLLQNVRSQIRL-ASSRKG 60
           MAVVSTSLASQ++      + + SG         NS  +Q +  QNV S +RL +SSR+ 
Sbjct: 1   MAVVSTSLASQITNPNSAVSTQFSGLRRSFLKLENSVSTQSSFFQNVDSHLRLSSSSRRC 60

Query: 61  SRGVVTMAGSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKD 120
            RGVV MAGSGK                          FFVGGNWKC             
Sbjct: 61  PRGVVAMAGSGK--------------------------FFVGGNWKC------------- 120

Query: 121 LWWMNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSK 173
               NGTKESI+KLV+DLN+A LE DVDVVVAPPFVYIDQVKSSL  R+EISAQN W+ K
Sbjct: 121 ----NGTKESITKLVSDLNSATLEADVDVVVAPPFVYIDQVKSSLTGRVEISAQNCWIGK 145

BLAST of CmoCh02G003250 vs. ExPASy Swiss-Prot
Match: Q9M4S8 (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.6e-31
Identity = 92/180 (51.11%), Postives = 106/180 (58.89%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVR-LSGSNSDISQTLLQNVRSQIRL-------ASSRKGSRGVVTMA 60
           MAV STSLASQLS  + LS   S + ++  +  +S   L       +SSRK SR VV MA
Sbjct: 1   MAVASTSLASQLSGPKSLSQPYSGLRRSCPKLDQSHSSLFQHLSLSSSSRKASRAVVAMA 60

Query: 61  GSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTK 120
           G+GK                          FFVGGNWKC                 NGTK
Sbjct: 61  GTGK--------------------------FFVGGNWKC-----------------NGTK 120

Query: 121 ESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           + ISKLV+DLN+AKLE DVDVVVAPPF+Y+DQVKSSL  RIEIS QNSWV+KGGAFTGEI
Sbjct: 121 DLISKLVSDLNSAKLEPDVDVVVAPPFLYLDQVKSSLTDRIEISGQNSWVAKGGAFTGEI 137

BLAST of CmoCh02G003250 vs. ExPASy Swiss-Prot
Match: Q9SKP6 (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.5e-28
Identity = 84/181 (46.41%), Postives = 95/181 (52.49%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDIS-----QTLLQNVRSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R      D +     Q+    V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGT 120
           AGSGK                          FFVGGNWKC                 NGT
Sbjct: 61  AGSGK--------------------------FFVGGNWKC-----------------NGT 120

Query: 121 KESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGE 173
           K+SI+KL++DLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+IS QNSWV KGGAFTGE
Sbjct: 121 KDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQNSWVGKGGAFTGE 138

BLAST of CmoCh02G003250 vs. ExPASy Swiss-Prot
Match: P46225 (Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.3e-27
Identity = 62/91 (68.13%), Postives = 68/91 (74.73%), Query Frame = 0

Query: 82  RFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVADLNNAKLEDDVDVVVAPPFVY 141
           +FFVGGNWKC                 NGTKESISKLV+DLN A LE DVDVVVAPPF+Y
Sbjct: 48  KFFVGGNWKC-----------------NGTKESISKLVSDLNAATLESDVDVVVAPPFIY 107

Query: 142 IDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           IDQVKSSL  RIE+SAQN+W+ KGGAFTGEI
Sbjct: 108 IDQVKSSLTDRIEVSAQNTWIGKGGAFTGEI 121

BLAST of CmoCh02G003250 vs. ExPASy Swiss-Prot
Match: P48491 (Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana OX=3702 GN=CTIMC PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-16
Identity = 45/98 (45.92%), Postives = 61/98 (62.24%), Query Frame = 0

Query: 82  RFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVADLNNAKL--EDDVDVVVAPPF 141
           +FFVGGNWKC                 NGT E + K+V  LN A++  +D V+VVV+PP+
Sbjct: 4   KFFVGGNWKC-----------------NGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPY 63

Query: 142 VYIDQVKSSLASRIEISAQNSWVSKGGAFTGEIRLILL 178
           V++  VKS+L S   ++AQN WV KGGAFTGE+   +L
Sbjct: 64  VFLPLVKSTLRSDFFVAAQNCWVKKGGAFTGEVSAEML 84

BLAST of CmoCh02G003250 vs. ExPASy TrEMBL
Match: A0A6J1G6X6 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451334 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.2e-53
Identity = 129/172 (75.00%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESISKLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESISKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. ExPASy TrEMBL
Match: A0A6J1L7Y2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111500005 PE=3 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.2e-52
Identity = 127/172 (73.84%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESI+KLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESITKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVD+VVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDIVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. ExPASy TrEMBL
Match: A0A6J1CXY1 (triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015469 PE=3 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 2.2e-49
Identity = 122/172 (70.93%), Postives = 124/172 (72.09%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLS S SDISQTL QNV SQIRLASSRKGSRGV TMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVFTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESI+KLV+
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESIAKLVS 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. ExPASy TrEMBL
Match: A0A6J1JFM2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111484585 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 3.1e-48
Identity = 119/172 (69.19%), Postives = 123/172 (71.51%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSA+RLS S SDISQTL QNV SQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESI+KLV+
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESITKLVS 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNN KLEDDVDVVVAPPF+YIDQVKSSLA RIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. ExPASy TrEMBL
Match: A0A6J1FWW5 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448968 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.7e-47
Identity = 117/172 (68.02%), Postives = 122/172 (70.93%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSA+RLS S SDISQTL QNV SQIRLASSRKG RGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGPRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESI+KLV+
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESITKLVS 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNN KLEDDVDVVVAPPF+YIDQV+SSLA RIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNVKLEDDVDVVVAPPFLYIDQVRSSLAPRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. NCBI nr
Match: KAG7035038.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 217.6 bits (553), Expect = 8.8e-53
Identity = 129/172 (75.00%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESISKLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESISKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. NCBI nr
Match: XP_022947479.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 217.6 bits (553), Expect = 8.8e-53
Identity = 129/172 (75.00%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESISKLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESISKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. NCBI nr
Match: KAG6605006.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 217.6 bits (553), Expect = 8.8e-53
Identity = 129/172 (75.00%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESISKLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESISKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. NCBI nr
Match: XP_023007543.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 216.1 bits (549), Expect = 2.6e-52
Identity = 127/172 (73.84%), Postives = 129/172 (75.00%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK    
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESI+KLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESITKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVD+VVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDIVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. NCBI nr
Match: XP_023533234.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 213.0 bits (541), Expect = 2.2e-51
Identity = 126/172 (73.26%), Postives = 127/172 (73.84%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKVLNL 60
           MAVVSTSLASQLS+VRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVV MAGSGK    
Sbjct: 1   MAVVSTSLASQLSSVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVAMAGSGK---- 60

Query: 61  FSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVA 120
                                 FFVGGNWKC                 NGTKESISKLVA
Sbjct: 61  ----------------------FFVGGNWKC-----------------NGTKESISKLVA 120

Query: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGEI 173
           DLNNAKLEDDVDVVVAPPFVYIDQVKSSL SRIEISAQNSWVSKGGAFTGEI
Sbjct: 121 DLNNAKLEDDVDVVVAPPFVYIDQVKSSLTSRIEISAQNSWVSKGGAFTGEI 129

BLAST of CmoCh02G003250 vs. TAIR 10
Match: AT2G21170.1 (triosephosphate isomerase )

HSP 1 Score: 126.3 bits (316), Expect = 2.5e-29
Identity = 84/181 (46.41%), Postives = 95/181 (52.49%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDIS-----QTLLQNVRSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R      D +     Q+    V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGT 120
           AGSGK                          FFVGGNWKC                 NGT
Sbjct: 61  AGSGK--------------------------FFVGGNWKC-----------------NGT 120

Query: 121 KESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGE 173
           K+SI+KL++DLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+IS QNSWV KGGAFTGE
Sbjct: 121 KDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQNSWVGKGGAFTGE 138

BLAST of CmoCh02G003250 vs. TAIR 10
Match: AT2G21170.2 (triosephosphate isomerase )

HSP 1 Score: 104.0 bits (258), Expect = 1.3e-22
Identity = 75/181 (41.44%), Postives = 86/181 (47.51%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSGSNSDIS-----QTLLQNVRSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R      D +     Q+    V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKVLNLFSLVYSRSALESFRLPEEFQIRFFVGGNWKCVSIFIIDNLIAKDLWWMNGT 120
           AGSGK                                                    NGT
Sbjct: 61  AGSGK----------------------------------------------------NGT 120

Query: 121 KESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVSKGGAFTGE 173
           K+SI+KL++DLN+A LE DVDVVV+PPFVYIDQVKSSL  RI+IS QNSWV KGGAFTGE
Sbjct: 121 KDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRIDISGQNSWVGKGGAFTGE 129

BLAST of CmoCh02G003250 vs. TAIR 10
Match: AT3G55440.1 (triosephosphate isomerase )

HSP 1 Score: 86.3 bits (212), Expect = 2.8e-17
Identity = 45/98 (45.92%), Postives = 61/98 (62.24%), Query Frame = 0

Query: 82  RFFVGGNWKCVSIFIIDNLIAKDLWWMNGTKESISKLVADLNNAKL--EDDVDVVVAPPF 141
           +FFVGGNWKC                 NGT E + K+V  LN A++  +D V+VVV+PP+
Sbjct: 4   KFFVGGNWKC-----------------NGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPY 63

Query: 142 VYIDQVKSSLASRIEISAQNSWVSKGGAFTGEIRLILL 178
           V++  VKS+L S   ++AQN WV KGGAFTGE+   +L
Sbjct: 64  VFLPLVKSTLRSDFFVAAQNCWVKKGGAFTGEVSAEML 84

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P484968.0e-3351.60Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 P... [more]
Q9M4S82.6e-3151.11Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=... [more]
Q9SKP63.5e-2846.41Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM ... [more]
P462252.3e-2768.13Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1[more]
P484914.0e-1645.92Triosephosphate isomerase, cytosolic OS=Arabidopsis thaliana OX=3702 GN=CTIMC PE... [more]
Match NameE-valueIdentityDescription
A0A6J1G6X64.2e-5375.00triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1L7Y21.2e-5273.84triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1CXY12.2e-4970.93triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1JFM23.1e-4869.19triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FWW52.7e-4768.02triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
KAG7035038.18.8e-5375.00Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_022947479.18.8e-5375.00triosephosphate isomerase, chloroplastic-like [Cucurbita moschata][more]
KAG6605006.18.8e-5375.00Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_023007543.12.6e-5273.84triosephosphate isomerase, chloroplastic-like [Cucurbita maxima][more]
XP_023533234.12.2e-5173.26triosephosphate isomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G21170.12.5e-2946.41triosephosphate isomerase [more]
AT2G21170.21.3e-2241.44triosephosphate isomerase [more]
AT3G55440.12.8e-1745.92triosephosphate isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 80..179
e-value: 3.8E-20
score: 74.3
IPR000652Triosephosphate isomerasePFAMPF00121TIMcoord: 106..174
e-value: 1.1E-12
score: 47.9
IPR000652Triosephosphate isomerasePANTHERPTHR21139TRIOSEPHOSPHATE ISOMERASEcoord: 106..176
coord: 5..57
IPR000652Triosephosphate isomerasePROSITEPS51440TIM_2coord: 99..183
score: 17.642637
NoneNo IPR availablePANTHERPTHR21139:SF20TRIOSEPHOSPHATE ISOMERASE, CHLOROPLASTICcoord: 106..176
NoneNo IPR availablePANTHERPTHR21139:SF20TRIOSEPHOSPHATE ISOMERASE, CHLOROPLASTICcoord: 5..57
IPR035990Triosephosphate isomerase superfamilySUPERFAMILY51351Triosephosphate isomerase (TIM)coord: 106..177

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G003250.1CmoCh02G003250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0004807 triose-phosphate isomerase activity
molecular_function GO:0003824 catalytic activity