CmoCh02G002870 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G002870
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionFlavin-containing monooxygenase
LocationCmo_Chr02: 1424176 .. 1427905 (-)
RNA-Seq ExpressionCmoCh02G002870
SyntenyCmoCh02G002870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAACCGAAGAAAGTGGCGATCATCGGAGCCGGAGTAAGCGGACTAGTGGCCTGCAAATTCACTCTATCCAAAGGATTCATTCCGATCGTCTTCGAGGCGAGAGGCGCCATTGGCGGTGTTTGGACCGAAACGCTGCAAACGCCACGAGAGATCTACCAGTTCTCCGATTTCCCGTGGCCGAAATCGGTGACCGAAGAATTTCCGAAGTACAATCAAGTTCTCGATTACATCAAATCTTACGCCGATCATTTCGGATTGATGAAGCACATCAGATTGAACACCAGAGTCCTGAGTATCGACTATGAAGGCTGCTCCGATGAGGAACTTGAAGGTTCGACTCTTTGGGGTGGCTCCGGCGAGGCTTTCGGTGAACGCCGTAAATGGAGGCTCAATGTCGTTGATGCTCAGACCAATGATCCACCCCAGGTGAGTTTATCATTTTCATTTTTCCATTTTTTTTGAACTTTTAATTTTATATTTTATAACATATAAACTTGTCCAATTTTTTTTTTTTTTTTAAATTCGAGAAAGTAATTAATTTTTTATTTTTATTTAATTAAATTATCAAATCTCCAAAAAAAAAATTAAAAATTAAAAATATTATTATATTATCCATTTAAATACCCTTTTAATTTAATCATCTAAGTTGAAGATCTATTATTAAAATTTAACTTTTATAATATAAAATACCTTTAAATTATTTATTATTCTTTTTTATTCTAGAATATAACATCGAGAATGATATAACGAATAATTATTTATTCTATGATATGAATTTATTATTAATTTCTCCGAATATTTTATGAAATTAAGTATAATAAAGTTAGATATATTATTATTATTATTTTTTTTTTTTTTAAACGTAAAATGTTGGAATAAAGAGGAGAGAATGGACGCAAGATTCTTTGGTAGAAAATGGTAAAAACCAAAGGAATAATATAACGTTCATATATTCCTAATTTGGCAAGAGAATAATTTTATTTGAAAAAAAAAATGGACAAAATTATTTGTTATCTATAATATAAAATCTAATATAATAATAAGAAGAATAAACCCGAGCTAGTTTAAATATACGTCTTTGACCAAACAACGTCATACTTTTGAATTAGGGCAAGATATTGATAAATTAAATTATATTCAAAGCTTAACTATAGATATCCTTTTACCCGTCCCAAATGAGGAGACGATTCTGCGTTTAAATGGTGCATAGGGAAGGGAGTGAGAAACATTTCTTTTTTTATTAGATAAATGGAACCCGAGATGGGACTATATTTCCTGTCCATATATATATATTTTACCTTATTATAAATATATTCTAATGATAATTTATGGTTTTTAATTGTTATTAATTATTATGTTATAATATTAAAATTTAAATTTTTAAAATTAGGTTAGGAATTATTATTTGGTTAAGAAATATATGGATAAAAAAATATATATTAAAAAGGTATATAATATATATATATATATATATATATATATATATATATACTGCTAAGGACAAGGGCGTGGGGATTCGCAGGAGACTGTGGTGGACTTTGTTATACTCTGCGTTGGAAGATTCAGCGACATTCCGAACATCCCTGAATTCCCTCCTAATGGCGGCCCTGAAGCTTTCAAATCCGGCAAAGTTCTTCACTCTCTCCAGTATTCCGCCATGGATTTCGACACTGCTTCCAACCTCATCAAGGACAAGCAAGTCATCGTCGTCGGTCTCCATAAGACAGCTCTTGATCTCGCCATGGAATGCGCCAACGCCAATGGTGATTTTCCCGCGATTTCTCTTTTTTCTCTCTCTCTTTCTCAGTTCCTTATTCTTCTTTTTCTCCGATTTGTAGGTCCGAAGAATCCCTGTACTGTTCTCTATAGAAACGCGCACTGGAACATTCCTGATCGTCATCCATGGGGAATCCCTTTGTCTTTTCTCTATATGAATCGATTTGCCGAGCTTCTCATTCACAAGCCTGGCGAGAGCTTTCTCCTCTATCTCCTCGCGATTATTCTTTCCCCAATTGTAATCATCGCCTCTCTCTCTCTCTATGTTTTTGTCTGTTTTGAATCTCTAGGGGTTTTATTGTGGTTTTTTTTTTTGTAGAGATGGTTGTTCGCGAAGATTGTGGAAACCTACGTAACGAGGAAGCTCGGAATGGCCAAATACGGGATGGTTCCAACCCACGGCTTTCTGCAAGACATCAGTTCATGTTTAATTGCAATCGTGCCTGAGAAATTCTACGAAAAAGTGGACGAAGGAAGCATTATATTGAAGAAATCACAGAGTTTCTGGTTCTGTGAAGACGGTATTATGATTAAAGGAGAAACCAAACCGATTCGCTCAGATTTGGTCATTTTGGCTACTGGATTTAGAGGTGATCAAAAGCTCAAACAAATCTTTACTTCCGCTACTTTTCGGGATTGCATGACATTTAAGGATTCAGTGATTCCCTTGTACAGGTTAGTCTTTCGTTTGAATCTATATCCCTGTTTCACCCCCTTCAACTTTTGAATTGCTATGACATTATCTTGAATTGTTTAGTTACTTAAAGCTGGTGAAAGATGAAACTAGTATGCTATTTGAGATATTGCACTTATATCAGACTAGAATATTGGTAGGAATTCATCTCATATAATTGTAGTCTAAGGTGGAACATCTGCAATGACCCAAGTAAAAATGACCTTCTTTAACCAAATCCACGCTAGTCTAGTAGAGTTGTCGAAAAAATTATTCAATGTAGCTGCCTCTTGATACGAGTTAAATGTAGATTCGAAATCAAGTATTTGAAATATTTACTTGCAAGTTCTCTCGCATATATATAATAGTACGTAATTTATGAGATCCCATATCGGTTGGGGAAGAGAAGGAAACATTCTTTACAAGGGTGTGGAAAACTCTTCAACCTTGAGGGAAAGTCCAAAAGGGAAAACTAGAGAAGGAAAGTCCAAAGAGGACAATATCTACTAGCAGTGGGCTTGAGTGACTCTCTCCAATCATTTCTCCAGATGGGTAGCAAAGAGACTGAAAACATGTGTGGCCTATTGAAACTTTGATTCTTATTCCTTCATATTGGATTTCTATCAGGTTCTCAGTTGTTGTAATAGGATTCTTTGAGTGTCTAGAGGAAGTTTATGGGGCTATTAACGTGGATTACTTAATGACAATGTGCTACGTTTCCTTCACGTATTAGAATTAGTAAAAAGAAAGTGTATCTATATTGCCTTCTACTGTGGCTTTACAATGAGGTATTGGTTGAGAACTGCACATGTTCTTGCGCTTGATGAGTTTATCATTCGCTTCAATTAAAATCTGATCGATGTTTGCATTTCATGTCATTCTCTGTTAGGAATACAGAATCTTAGTGATCACATGTTTATGTCACATTTGATCAGGCATTGCATTCATCCCAGAATCCCACAGCTAGCTGTGATTGGGTTTACAGAAAGCCCTTCCAACTTGTTCGCCTCCGAGATGCGGTGCCGATGGGTGGCGGAGTTTCTGGACGGCACGTTCAAGCTACCAAGCATCAAGGAGATGGAGAAGGACATAGCCAAATGGGAGAAGAGCATGACGCGTTACTCAGGCCCTTTCTCCAAGCGAGCTTGCCTTGCCATTGTGAATATTTGGTACAATGATCAGCTCTGCAAGGACATGGGGTGGAACCCCATGAGGAAAAAGGGCTTTTTTGCTAACTTGTTTGTGCCTTATGGTCCTTCAGATTATGCCTCCCCTTGA

mRNA sequence

ATGGAACCGAAGAAAGTGGCGATCATCGGAGCCGGAGTAAGCGGACTAGTGGCCTGCAAATTCACTCTATCCAAAGGATTCATTCCGATCGTCTTCGAGGCGAGAGGCGCCATTGGCGGTGTTTGGACCGAAACGCTGCAAACGCCACGAGAGATCTACCAGTTCTCCGATTTCCCGTGGCCGAAATCGGTGACCGAAGAATTTCCGAAGTACAATCAAGTTCTCGATTACATCAAATCTTACGCCGATCATTTCGGATTGATGAAGCACATCAGATTGAACACCAGAGTCCTGAGTATCGACTATGAAGGCTGCTCCGATGAGGAACTTGAAGGTTCGACTCTTTGGGGTGGCTCCGGCGAGGCTTTCGGTGAACGCCGTAAATGGAGGCTCAATGTCGTTGATGCTCAGACCAATGATCCACCCCAGGAGACTGTGGTGGACTTTGTTATACTCTGCGTTGGAAGATTCAGCGACATTCCGAACATCCCTGAATTCCCTCCTAATGGCGGCCCTGAAGCTTTCAAATCCGGCAAAGTTCTTCACTCTCTCCAGTATTCCGCCATGGATTTCGACACTGCTTCCAACCTCATCAAGGACAAGCAAGTCATCGTCGTCGGTCTCCATAAGACAGCTCTTGATCTCGCCATGGAATGCGCCAACGCCAATGGTCCGAAGAATCCCTGTACTGTTCTCTATAGAAACGCGCACTGGAACATTCCTGATCGTCATCCATGGGGAATCCCTTTGTCTTTTCTCTATATGAATCGATTTGCCGAGCTTCTCATTCACAAGCCTGGCGAGAGCTTTCTCCTCTATCTCCTCGCGATTATTCTTTCCCCAATTAGATGGTTGTTCGCGAAGATTGTGGAAACCTACGTAACGAGGAAGCTCGGAATGGCCAAATACGGGATGGTTCCAACCCACGGCTTTCTGCAAGACATCAGTTCATGTTTAATTGCAATCGTGCCTGAGAAATTCTACGAAAAAGTGGACGAAGGAAGCATTATATTGAAGAAATCACAGAGTTTCTGGTTCTGTGAAGACGGTATTATGATTAAAGGAGAAACCAAACCGATTCGCTCAGATTTGGTCATTTTGGCTACTGGATTTAGAGGTGATCAAAAGCTCAAACAAATCTTTACTTCCGCTACTTTTCGGGATTGCATGACATTTAAGGATTCAGTGATTCCCTTGTACAGGCATTGCATTCATCCCAGAATCCCACAGCTAGCTGTGATTGGGTTTACAGAAAGCCCTTCCAACTTGTTCGCCTCCGAGATGCGGTGCCGATGGGTGGCGGAGTTTCTGGACGGCACGTTCAAGCTACCAAGCATCAAGGAGATGGAGAAGGACATAGCCAAATGGGAGAAGAGCATGACGCGTTACTCAGGCCCTTTCTCCAAGCGAGCTTGCCTTGCCATTGTGAATATTTGGTACAATGATCAGCTCTGCAAGGACATGGGGTGGAACCCCATGAGGAAAAAGGGCTTTTTTGCTAACTTGTTTGTGCCTTATGGTCCTTCAGATTATGCCTCCCCTTGA

Coding sequence (CDS)

ATGGAACCGAAGAAAGTGGCGATCATCGGAGCCGGAGTAAGCGGACTAGTGGCCTGCAAATTCACTCTATCCAAAGGATTCATTCCGATCGTCTTCGAGGCGAGAGGCGCCATTGGCGGTGTTTGGACCGAAACGCTGCAAACGCCACGAGAGATCTACCAGTTCTCCGATTTCCCGTGGCCGAAATCGGTGACCGAAGAATTTCCGAAGTACAATCAAGTTCTCGATTACATCAAATCTTACGCCGATCATTTCGGATTGATGAAGCACATCAGATTGAACACCAGAGTCCTGAGTATCGACTATGAAGGCTGCTCCGATGAGGAACTTGAAGGTTCGACTCTTTGGGGTGGCTCCGGCGAGGCTTTCGGTGAACGCCGTAAATGGAGGCTCAATGTCGTTGATGCTCAGACCAATGATCCACCCCAGGAGACTGTGGTGGACTTTGTTATACTCTGCGTTGGAAGATTCAGCGACATTCCGAACATCCCTGAATTCCCTCCTAATGGCGGCCCTGAAGCTTTCAAATCCGGCAAAGTTCTTCACTCTCTCCAGTATTCCGCCATGGATTTCGACACTGCTTCCAACCTCATCAAGGACAAGCAAGTCATCGTCGTCGGTCTCCATAAGACAGCTCTTGATCTCGCCATGGAATGCGCCAACGCCAATGGTCCGAAGAATCCCTGTACTGTTCTCTATAGAAACGCGCACTGGAACATTCCTGATCGTCATCCATGGGGAATCCCTTTGTCTTTTCTCTATATGAATCGATTTGCCGAGCTTCTCATTCACAAGCCTGGCGAGAGCTTTCTCCTCTATCTCCTCGCGATTATTCTTTCCCCAATTAGATGGTTGTTCGCGAAGATTGTGGAAACCTACGTAACGAGGAAGCTCGGAATGGCCAAATACGGGATGGTTCCAACCCACGGCTTTCTGCAAGACATCAGTTCATGTTTAATTGCAATCGTGCCTGAGAAATTCTACGAAAAAGTGGACGAAGGAAGCATTATATTGAAGAAATCACAGAGTTTCTGGTTCTGTGAAGACGGTATTATGATTAAAGGAGAAACCAAACCGATTCGCTCAGATTTGGTCATTTTGGCTACTGGATTTAGAGGTGATCAAAAGCTCAAACAAATCTTTACTTCCGCTACTTTTCGGGATTGCATGACATTTAAGGATTCAGTGATTCCCTTGTACAGGCATTGCATTCATCCCAGAATCCCACAGCTAGCTGTGATTGGGTTTACAGAAAGCCCTTCCAACTTGTTCGCCTCCGAGATGCGGTGCCGATGGGTGGCGGAGTTTCTGGACGGCACGTTCAAGCTACCAAGCATCAAGGAGATGGAGAAGGACATAGCCAAATGGGAGAAGAGCATGACGCGTTACTCAGGCCCTTTCTCCAAGCGAGCTTGCCTTGCCATTGTGAATATTTGGTACAATGATCAGCTCTGCAAGGACATGGGGTGGAACCCCATGAGGAAAAAGGGCTTTTTTGCTAACTTGTTTGTGCCTTATGGTCCTTCAGATTATGCCTCCCCTTGA

Protein sequence

MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP
Homology
BLAST of CmoCh02G002870 vs. ExPASy Swiss-Prot
Match: Q9LMA1 (Probable flavin-containing monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=FMO1 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 9.9e-113
Identity = 212/516 (41.09%), Postives = 306/516 (59.30%), Query Frame = 0

Query: 5   KVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW------TETLQTPREIYQFSDF 64
           +VAIIGAGVSGL A K  +     P VFEA  ++GGVW      T  LQ+ R  Y+FSDF
Sbjct: 12  RVAIIGAGVSGLAAAKNLVHHN--PTVFEASDSVGGVWRSCTYETTKLQSARVDYEFSDF 71

Query: 65  PWPKSVTE-EFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWG 124
           PWP +  +  FP Y ++LDY++SYA HF L+K ++  ++V+ + + G  D E       G
Sbjct: 72  PWPNNRDDTTFPPYLEILDYLESYAKHFDLLKFMKFGSKVIEVRFIG--DGETPQMVDLG 131

Query: 125 GSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKS 184
             G     +  W + V    + D  Q    +FV++C G++ D+P IP FP   GPE F+ 
Sbjct: 132 AYGNLLPGKPVWEVAVQIGDSGD-IQWHAFEFVVVCTGKYGDVPRIPAFPAKKGPEMFQ- 191

Query: 185 GKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPK--NPCTVLYRN 244
           GKV+HS+ Y  ++ + AS L+  K+V V+G  K+A+DLA+E A AN  +    CT++ R 
Sbjct: 192 GKVMHSMDYCKLEKEEASTLLSGKKVAVIGFKKSAIDLALESALANQGEGGKACTMVVRT 251

Query: 245 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 304
            HW IP    WG+P    Y +R ++ L  +P +SFL  L  ++ S +R + +K +E+YV 
Sbjct: 252 THWGIPHYWVWGLPFFLFYSSRASQFLHDRPNQSFLRTLFCLLFSLLRAVVSKFIESYVL 311

Query: 305 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 364
            KL + KYG+ P H F +D +SC +AI+PE F+E+ D+G I  KKS  +WF E+GI+ + 
Sbjct: 312 WKLPLEKYGLKPNHSFEEDYASCQMAIIPENFFEEADKGMIRFKKSSKWWFYEEGIVFED 371

Query: 365 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 424
            T  + +D+VILATG+ G +KLK I     FR  + F   V+PLYR  IHP IP +  +G
Sbjct: 372 GT-TLEADVVILATGYDGKKKLKAI-VPEPFRTWLEFPSGVMPLYRGTIHPLIPNMGFVG 431

Query: 425 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 484
           + +S SNL  SE+R  W++  +D  F+LPS ++M     K E  +TR S  F KR C++ 
Sbjct: 432 YVQSSSNLHTSELRSMWLSRLVDEKFRLPSKEKMLDQFLK-EMEVTRNSSRFYKRHCIST 491

Query: 485 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDY 512
            +I + D +C DMG NP RK  F    F PYG  DY
Sbjct: 492 FSIQHADDMCNDMGLNPWRKSNFLLEAFSPYGSQDY 518

BLAST of CmoCh02G002870 vs. ExPASy Swiss-Prot
Match: Q9FKE7 (Putative flavin-containing monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=FMO2 PE=3 SV=2)

HSP 1 Score: 330.9 bits (847), Expect = 2.6e-89
Identity = 196/501 (39.12%), Postives = 273/501 (54.49%), Query Frame = 0

Query: 5   KVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW------TETLQTPREIYQFSDF 64
           +VAIIGAGVSGL A K        P VFEA  +IGGVW      T  LQ+ R  Y+ SDF
Sbjct: 12  RVAIIGAGVSGLAAAKHLARHH--PQVFEASDSIGGVWRKCTYETTKLQSVRVSYELSDF 71

Query: 65  PWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGG 124
            WP      FP Y  VLDY+++YA HF L+K I+ N++V+ + + G      +G TL  G
Sbjct: 72  LWPNRGESSFPTYVDVLDYLEAYAKHFNLVKFIKFNSKVVELRFIG------DGKTLQMG 131

Query: 125 SGEAFGE----RRKWRLNVVDAQTNDPP-QETVVDFVILCVGRFSDIPNIPEFPPNGGPE 184
              A+G     +  W + V    T D   Q    ++V++C G++ D+P  P FP   GPE
Sbjct: 132 DLGAYGNLLPGKPVWEVAV---NTGDGDIQWHAFEYVVVCAGKYGDVPRTPTFPVKKGPE 191

Query: 185 AFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPK--NPCTV 244
            FK GKVLHS+ YS +  + AS L+  K+V V+G  K+A+DLA+E A AN  K    CT+
Sbjct: 192 IFK-GKVLHSMDYSKLQKEKASQLLHGKKVAVIGFKKSAIDLALESALANQGKEGKTCTM 251

Query: 245 LYRNAHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVE 304
           + R  HW IP  H W                                    R   +K +E
Sbjct: 252 VVRTPHWVIP--HYW------------------------------------RATVSKFIE 311

Query: 305 TYVTRKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGI 364
           +YV  KL + KYG+ P H F +D +SC +A+VPEKF+E+ D+G I  K++ ++WF ++GI
Sbjct: 312 SYVLWKLPLEKYGLKPDHAFEEDYASCQMALVPEKFFEEADKGMIRFKRTTNWWFYDEGI 371

Query: 365 MIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQL 424
             +  T  + +D+VILATG+ G +KLK I     FR  + F   ++PLYR  IHP IP +
Sbjct: 372 EFEDGT-TLEADVVILATGYDGMKKLKAI-VPEPFRSWLEFPWGIMPLYRGTIHPLIPNM 431

Query: 425 AVIGFTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRA 484
             IG+ +S SNL +SE+  RW+++ LDG F LPS ++M     K E  + R S  F K  
Sbjct: 432 GFIGYVQSSSNLKSSELHSRWLSQLLDGKFTLPSKEKMLDQFLK-EMHVMRRSSRFFKNH 459

Query: 485 CLAIVNIWYNDQLCKDMGWNP 493
           C +  +I + D L KDM   P
Sbjct: 492 CFSTFSIQHADDLSKDMNLKP 459

BLAST of CmoCh02G002870 vs. ExPASy Swiss-Prot
Match: Q01740 (Dimethylaniline monooxygenase [N-oxide-forming] 1 OS=Homo sapiens OX=9606 GN=FMO1 PE=1 SV=3)

HSP 1 Score: 146.4 bits (368), Expect = 9.1e-34
Identity = 127/475 (26.74%), Postives = 211/475 (44.42%), Query Frame = 0

Query: 4   KKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW--TETLQTPR----------- 63
           K+VAI+GAGVSGL + K  L +G  P  FE    +GG+W  TE ++  R           
Sbjct: 3   KRVAIVGAGVSGLASIKCCLEEGLEPTCFERSDDLGGLWRFTEHVEEGRASLYKSVVSNS 62

Query: 64  --EIYQFSDFPWPKSVTEEFPKY---NQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGC 123
             E+  +SDFP+P    E++P Y   +Q L+Y+K YA+HF L+KHI+  T+V S+    C
Sbjct: 63  CKEMSCYSDFPFP----EDYPNYVPNSQFLEYLKMYANHFDLLKHIQFKTKVCSV--TKC 122

Query: 124 SDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIP- 183
           SD  + G               +W    V     +  +  + D V++C G F   P +P 
Sbjct: 123 SDSAVSG---------------QWE---VVTMHEEKQESAIFDAVMVCTG-FLTNPYLPL 182

Query: 184 -EFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANAN 243
             FP   G  AFK G+  HS QY   D      + KDK+V+V+G+  +  D+A+E ++  
Sbjct: 183 DSFP---GINAFK-GQYFHSRQYKHPD------IFKDKRVLVIGMGNSGTDIAVEASHL- 242

Query: 244 GPKNPCTVLYRNAHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIR 303
                  +      W I      G P   ++M RF  +L +           ++    + 
Sbjct: 243 --AEKVFLSTTGGGWVISRIFDSGYPWDMVFMTRFQNMLRN-----------SLPTPIVT 302

Query: 304 WLFAKIVETYVTRKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQS 363
           WL    +E  +   L  A YG++P     +D +     ++ ++   ++  G + ++ S  
Sbjct: 303 WL----MERKINNWLNHANYGLIP-----EDRTQLKEFVLNDELPGRIITGKVFIRPSIK 362

Query: 364 FWFCEDGIMIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRD--CMTFKDSVIPLYR 423
               E+ ++    +K    D+++ ATG+           +  F D   +  +D    LY+
Sbjct: 363 -EVKENSVIFNNTSKEEPIDIIVFATGYT---------FAFPFLDESVVKVEDGQASLYK 409

Query: 424 HCI--HPRIPQLAVIGFTESPSNLF-ASEMRCRWVAEFLDGTFKLPSIKEMEKDI 454
           +    H + P LA+IG  +   ++    E + RW    L G  KLP    M ++I
Sbjct: 423 YIFPAHLQKPTLAIIGLIKPLGSMIPTGETQARWAVRVLKGVNKLPPPSVMIEEI 409

BLAST of CmoCh02G002870 vs. ExPASy Swiss-Prot
Match: Q5REK0 (Dimethylaniline monooxygenase [N-oxide-forming] 2 OS=Pongo abelii OX=9601 GN=FMO2 PE=2 SV=3)

HSP 1 Score: 145.6 bits (366), Expect = 1.6e-33
Identity = 141/524 (26.91%), Postives = 224/524 (42.75%), Query Frame = 0

Query: 4   KKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW---------------TETLQT 63
           KKVA+IGAGVSGL++ K  + +G  P  FE    IGGVW               +    T
Sbjct: 3   KKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNT 62

Query: 64  PREIYQFSDFPWPKSVTEEFPKY---NQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGC 123
            +E+  FSDFP P    E+FP +   +++L+Y + +A  F L+K+I+  T VLS+    C
Sbjct: 63  SKEMSCFSDFPMP----EDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSV--RKC 122

Query: 124 SDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIP- 183
            D               F    +W+   V  Q+N   Q  V D V++C G    +P+IP 
Sbjct: 123 PD---------------FSSSGQWK---VVTQSNSKEQSAVFDAVMVCSGHHI-LPHIPL 182

Query: 184 -EFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANAN 243
             FP   G E FK G+  HS QY   D        + K+++V+G+  +  D+A+E +   
Sbjct: 183 KSFP---GIERFK-GQYFHSRQYKHPDG------FEGKRILVIGMGNSGSDIAVELS--- 242

Query: 244 GPKNPCTVLYRNAH--WNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSP 303
             KN   V     H  W +      G P   ++  RF  +L              +  + 
Sbjct: 243 --KNAAQVFISTRHGTWVLSRISEDGYPWDSVFHTRFRSMLRR-----------VLPRTA 302

Query: 304 IRWLFAKIVETYVTRKLGMAKYGMVPTHGFL-------QDISSCLI--AIVPEKFYEKVD 363
           ++W+    +E  + R      YG+ P + +         D+ S L+  AI  +   +++ 
Sbjct: 303 VKWM----IEQQMNRWFNHENYGLEPQNKYFMKEPVLNDDVPSRLLCGAIKVKSTVKELT 362

Query: 364 EGSIILKKSQSFWFCEDGIMIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMT- 423
           E S I          EDG      T     D++I ATG+         F+     D +  
Sbjct: 363 ETSAIF---------EDG------TVEENIDVIIFATGYS--------FSFPFLEDSLVK 422

Query: 424 FKDSVIPLYRHCI--HPRIPQLAVIGFTESPSNLF-ASEMRCRWVAEFLDGTFKLPSIKE 483
            +++++ LY++    H     LA IG  +   ++F  +E++ RWV     G   LPS + 
Sbjct: 423 VENNMVSLYKYIFPAHLEKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERT 447

Query: 484 MEKDIAKWEKSMTRYSGPFSKRACLAIVNIWYNDQLCKDMGWNP 493
           M  DI K  +      G  S+   L    + Y D+L  ++G  P
Sbjct: 483 MMMDIIKRNEKRIELFGE-SQSQTLQTNYVDYLDELALEIGAKP 447

BLAST of CmoCh02G002870 vs. ExPASy Swiss-Prot
Match: Q99518 (Dimethylaniline monooxygenase [N-oxide-forming] 2 OS=Homo sapiens OX=9606 GN=FMO2 PE=1 SV=5)

HSP 1 Score: 145.2 bits (365), Expect = 2.0e-33
Identity = 140/524 (26.72%), Postives = 227/524 (43.32%), Query Frame = 0

Query: 4   KKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW---------------TETLQT 63
           KKVA+IGAGVSGL++ K  + +G  P  FE    IGGVW               +    T
Sbjct: 3   KKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNT 62

Query: 64  PREIYQFSDFPWPKSVTEEFPKY---NQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGC 123
            +E+  FSDFP P    E+FP +   +++L+Y + +A  F L+K+I+  T VLS+    C
Sbjct: 63  SKEMSCFSDFPMP----EDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSV--RKC 122

Query: 124 SDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIP- 183
            D               F    +W+   V  Q+N   Q  V D V++C G    +P+IP 
Sbjct: 123 PD---------------FSSSGQWK---VVTQSNGKEQSAVFDAVMVCSGHHI-LPHIPL 182

Query: 184 -EFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANAN 243
             FP   G E FK G+  HS QY   D        + K+++V+G+  +  D+A+E +   
Sbjct: 183 KSFP---GMERFK-GQYFHSRQYKHPDG------FEGKRILVIGMGNSGSDIAVELS--- 242

Query: 244 GPKNPCTVLYRNAH--WNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSP 303
             KN   V     H  W +      G P   ++  RF  +L +            +  + 
Sbjct: 243 --KNAAQVFISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRN-----------VLPRTA 302

Query: 304 IRWLFAKIVETYVTRKLGMAKYGMVPTHGFL-------QDISSCLI--AIVPEKFYEKVD 363
           ++W+    +E  + R      YG+ P + ++        D+ S L+  AI  +   +++ 
Sbjct: 303 VKWM----IEQQMNRWFNHENYGLEPQNKYIMKEPVLNDDVPSRLLCGAIKVKSTVKELT 362

Query: 364 EGSIILKKSQSFWFCEDGIMIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMT- 423
           E S I          EDG      T     D++I ATG+         F+     D +  
Sbjct: 363 ETSAIF---------EDG------TVEENIDVIIFATGYS--------FSFPFLEDSLVK 422

Query: 424 FKDSVIPLYRHCIHPRIPQ--LAVIGFTESPSNLF-ASEMRCRWVAEFLDGTFKLPSIKE 483
            +++++ LY++     + +  LA IG  +   ++F  +E++ RWV     G   LPS + 
Sbjct: 423 VENNMVSLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERT 447

Query: 484 MEKDIAKWEKSMTRYSGPFSKRACLAIVNIWYNDQLCKDMGWNP 493
           M  DI K  +      G  S+   L    + Y D+L  ++G  P
Sbjct: 483 MMMDIIKRNEKRIDLFGE-SQSQTLQTNYVDYLDELALEIGAKP 447

BLAST of CmoCh02G002870 vs. ExPASy TrEMBL
Match: A0A6J1G877 (Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451755 PE=3 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW 60
           MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW
Sbjct: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW 60

Query: 61  PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG 120
           PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG
Sbjct: 61  PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG 120

Query: 121 EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV 180
           EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV
Sbjct: 121 EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV 180

Query: 181 LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI 240
           LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI
Sbjct: 181 LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI 240

Query: 241 PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM 300
           PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM
Sbjct: 241 PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM 300

Query: 301 AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI 360
           AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI
Sbjct: 301 AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI 360

Query: 361 RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP 420
           RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP
Sbjct: 361 RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP 420

Query: 421 SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY 480
           SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY
Sbjct: 421 SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY 480

Query: 481 NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP
Sbjct: 481 NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 514

BLAST of CmoCh02G002870 vs. ExPASy TrEMBL
Match: A0A6J1I4G0 (Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469790 PE=3 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 7.2e-284
Identity = 463/519 (89.21%), Postives = 491/519 (94.61%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL ACKFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAACKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPK+VTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+VLSID EGCSDEEL+G TL
Sbjct: 61  SDFPWPKTVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVLSIDCEGCSDEELDGWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAF ERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFSERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           K+GKVLHSLQYSAMDFDTASNLIKDKQV VVGL K+ALDLAMECANANGPKNPCT+LYR 
Sbjct: 181 KAGKVLHSLQYSAMDFDTASNLIKDKQVTVVGLQKSALDLAMECANANGPKNPCTLLYRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVD+GSIILKKSQSFWFCEDGIMI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDQGSIILKKSQSFWFCEDGIMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFDDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWV EFLDGTFKLPSIKEMEKDIA WEKS+T YSGPFSKRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVTEFLDGTFKLPSIKEMEKDIANWEKSLTYYSGPFSKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNP RKKGF ANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPKRKKGFIANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. ExPASy TrEMBL
Match: A0A6J1G8Q6 (Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451753 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 2.6e-281
Identity = 458/519 (88.25%), Postives = 489/519 (94.22%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL A KFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAASKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+V+SID EGCSD+E++  TL
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVMSIDCEGCSDDEIDRWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAFGERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFGERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFDTAS LIKDKQV VVGL K+ALDLAMECANANGPKNPCT+L+R 
Sbjct: 181 KSGKVLHSLQYSAMDFDTASKLIKDKQVTVVGLQKSALDLAMECANANGPKNPCTLLFRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLA+ILSPIRWLFAKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAVILSPIRWLFAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVDEGSIILKKSQSFWFCEDGIMI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDEGSIILKKSQSFWFCEDGIMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFNDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWV EFLDGTFKLPSIKEMEKDIA WE S+T YSGPF+KRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVVEFLDGTFKLPSIKEMEKDIANWENSLTYYSGPFAKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNPMRKKG FANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPMRKKGLFANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. ExPASy TrEMBL
Match: A0A6J1I223 (Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469791 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.6e-267
Identity = 433/519 (83.43%), Postives = 472/519 (90.94%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL ACKFTLSKGFIPIVFEARGAIGGVWTET     LQTP+++Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAACKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKDMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NTRVLSIDYEGCSDEEL+G  L
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTRVLSIDYEGCSDEELDGWNL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAFG+RRKWRLNVVDA TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFGDRRKWRLNVVDAHTNDPIEEVVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFD+AS LIK+KQV VVG  K+ALDLAMECAN NGPKNPCTVLY+ 
Sbjct: 181 KSGKVLHSLQYSAMDFDSASKLIKNKQVTVVGFQKSALDLAMECANTNGPKNPCTVLYKT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPLSFLYMNRFAELLIHKPGE+F+LYLLA++LSPIRW FAKIVET+  
Sbjct: 241 AHWNLPDNHPWGIPLSFLYMNRFAELLIHKPGENFILYLLAVLLSPIRWFFAKIVETHAK 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           +K  +AKYGM+PTH FLQDISSC +A +PEKFYEKVDEGSIILKKS +F FCE+GI I+G
Sbjct: 301 KKHKLAKYGMIPTHSFLQDISSCQLASLPEKFYEKVDEGSIILKKSPNFMFCEEGITIQG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           +TKPI SDLVILATGFRGDQKLKQIFTSAT R+ MTF+D+V PLYR CIHPRIPQLAV+G
Sbjct: 361 QTKPIHSDLVILATGFRGDQKLKQIFTSATHRNRMTFRDAVNPLYRQCIHPRIPQLAVVG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSN+F SE+RCRWVAEFL GTFKLPSIKEMEKDIA WEK +  YSGPFSKRAC A 
Sbjct: 421 FTESPSNMFTSEIRCRWVAEFLAGTFKLPSIKEMEKDIANWEKILKHYSGPFSKRACFAT 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           ++IWYNDQLC DMGWNP RKKGFFANLFVP+ P DYASP
Sbjct: 481 LHIWYNDQLCNDMGWNPKRKKGFFANLFVPFSPLDYASP 519

BLAST of CmoCh02G002870 vs. ExPASy TrEMBL
Match: A0A6J1G8N4 (Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451754 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.7e-267
Identity = 433/519 (83.43%), Postives = 472/519 (90.94%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL ACKFTLSKGFIPIVFEARGAIGGVWTET     LQTP+++Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAACKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKDMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NTRVLSIDYEGCSDEEL+   L
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTRVLSIDYEGCSDEELDSWNL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           W GSGEAFG+RRKWRLNVVDA TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WSGSGEAFGDRRKWRLNVVDAHTNDPIEEMVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFDTASNLIK+KQV VVG  K+ALDLAMECAN NGPKNPCTVLY+ 
Sbjct: 181 KSGKVLHSLQYSAMDFDTASNLIKNKQVTVVGFQKSALDLAMECANTNGPKNPCTVLYKT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPLSFLYMNRFAELLIHKPGE+F+LYLLA++LSPIRW FAKIVET+  
Sbjct: 241 AHWNLPDNHPWGIPLSFLYMNRFAELLIHKPGENFILYLLAVLLSPIRWFFAKIVETHAK 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           +K  +AKYGM+PTH FLQDISSC +A +PEKFYEKVDEGSIILKKS +F FCE+GI I+G
Sbjct: 301 KKHKLAKYGMIPTHSFLQDISSCQLASLPEKFYEKVDEGSIILKKSPNFMFCEEGITIQG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           +TKPIR+DLVILATGFRGDQKLKQIFTSAT R+ MTF+D+V PLYR CIHPRIPQLAVIG
Sbjct: 361 QTKPIRTDLVILATGFRGDQKLKQIFTSATHRNHMTFRDAVNPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSN+F SE+RCRWVAEFL GTFKLPSIKEMEKDIA WEK +  YSGPFSKRAC A 
Sbjct: 421 FTESPSNMFTSEIRCRWVAEFLAGTFKLPSIKEMEKDIANWEKILKHYSGPFSKRACFAT 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           ++IWYNDQLC D+GWNP RKKGFFANLFVP+ P DYASP
Sbjct: 481 LHIWYNDQLCNDIGWNPKRKKGFFANLFVPFSPLDYASP 519

BLAST of CmoCh02G002870 vs. NCBI nr
Match: XP_022948061.1 (probable flavin-containing monooxygenase 1 [Cucurbita moschata])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW 60
           MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW
Sbjct: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQTPREIYQFSDFPW 60

Query: 61  PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG 120
           PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG
Sbjct: 61  PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGGSG 120

Query: 121 EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV 180
           EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV
Sbjct: 121 EAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKSGKV 180

Query: 181 LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI 240
           LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI
Sbjct: 181 LHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRNAHWNI 240

Query: 241 PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM 300
           PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM
Sbjct: 241 PDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVTRKLGM 300

Query: 301 AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI 360
           AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI
Sbjct: 301 AKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKGETKPI 360

Query: 361 RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP 420
           RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP
Sbjct: 361 RSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIGFTESP 420

Query: 421 SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY 480
           SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY
Sbjct: 421 SNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAIVNIWY 480

Query: 481 NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP
Sbjct: 481 NDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 514

BLAST of CmoCh02G002870 vs. NCBI nr
Match: XP_022970985.1 (probable flavin-containing monooxygenase 1 [Cucurbita maxima])

HSP 1 Score: 985.7 bits (2547), Expect = 1.5e-283
Identity = 463/519 (89.21%), Postives = 491/519 (94.61%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL ACKFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAACKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPK+VTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+VLSID EGCSDEEL+G TL
Sbjct: 61  SDFPWPKTVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVLSIDCEGCSDEELDGWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAF ERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFSERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           K+GKVLHSLQYSAMDFDTASNLIKDKQV VVGL K+ALDLAMECANANGPKNPCT+LYR 
Sbjct: 181 KAGKVLHSLQYSAMDFDTASNLIKDKQVTVVGLQKSALDLAMECANANGPKNPCTLLYRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVD+GSIILKKSQSFWFCEDGIMI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDQGSIILKKSQSFWFCEDGIMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFDDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWV EFLDGTFKLPSIKEMEKDIA WEKS+T YSGPFSKRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVTEFLDGTFKLPSIKEMEKDIANWEKSLTYYSGPFSKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNP RKKGF ANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPKRKKGFIANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. NCBI nr
Match: XP_022948059.1 (probable flavin-containing monooxygenase 1 [Cucurbita moschata])

HSP 1 Score: 977.2 bits (2525), Expect = 5.3e-281
Identity = 458/519 (88.25%), Postives = 489/519 (94.22%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL A KFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAASKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+V+SID EGCSD+E++  TL
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVMSIDCEGCSDDEIDRWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAFGERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFGERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFDTAS LIKDKQV VVGL K+ALDLAMECANANGPKNPCT+L+R 
Sbjct: 181 KSGKVLHSLQYSAMDFDTASKLIKDKQVTVVGLQKSALDLAMECANANGPKNPCTLLFRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLA+ILSPIRWLFAKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAVILSPIRWLFAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVDEGSIILKKSQSFWFCEDGIMI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDEGSIILKKSQSFWFCEDGIMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFNDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWV EFLDGTFKLPSIKEMEKDIA WE S+T YSGPF+KRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVVEFLDGTFKLPSIKEMEKDIANWENSLTYYSGPFAKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNPMRKKG FANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPMRKKGLFANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. NCBI nr
Match: XP_023534050.1 (probable flavin-containing monooxygenase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 977.2 bits (2525), Expect = 5.3e-281
Identity = 459/519 (88.44%), Postives = 489/519 (94.22%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAIIGAGVSGL ACKFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIIGAGVSGLAACKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+V+SID EGCSDEE+ G TL
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVMSIDCEGCSDEEINGWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAF ERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFSERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFDTASNLIKDKQV VVGL K+ALDLAMECANANGP+NPCT+LYR 
Sbjct: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVTVVGLQKSALDLAMECANANGPRNPCTLLYRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLA+ILSPI  + AKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAVILSPIVIIIAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVDEGSIILKKSQSFWFCEDG+MI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDEGSIILKKSQSFWFCEDGVMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFHDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWVAEFLDGTFKLPSIKEMEKDIA WEKS+T YSGPFSKRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVAEFLDGTFKLPSIKEMEKDIANWEKSLTYYSGPFSKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNPMRKKG FANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPMRKKGLFANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. NCBI nr
Match: KAG6604966.1 (putative flavin-containing monooxygenase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 976.5 bits (2523), Expect = 9.1e-281
Identity = 459/519 (88.44%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 1   MEPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTET-----LQTPREIYQF 60
           MEPKKVAI+GAGVSGL A KFTLSKGFIPIVFEARGAIGGVWTET     LQTP+E+Y+F
Sbjct: 1   MEPKKVAIVGAGVSGLAASKFTLSKGFIPIVFEARGAIGGVWTETLQTTALQTPKEMYRF 60

Query: 61  SDFPWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTL 120
           SDFPWPKSVTEEFPKYNQVLDY+KSYADHFGLMKHIR NT+V+SID EGCSDEE++  TL
Sbjct: 61  SDFPWPKSVTEEFPKYNQVLDYLKSYADHFGLMKHIRFNTKVMSIDCEGCSDEEIDRWTL 120

Query: 121 WGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAF 180
           WGGSGEAFGERRKWRLNVVDA+TNDP +E VVDFVILC+GRFSD+PNIPEFPPNGGPEAF
Sbjct: 121 WGGSGEAFGERRKWRLNVVDARTNDPLEEIVVDFVILCIGRFSDVPNIPEFPPNGGPEAF 180

Query: 181 KSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPKNPCTVLYRN 240
           KSGKVLHSLQYSAMDFDTAS LIKDKQV VVGL K+ALDLAMECANANGPKNPCT+L+R 
Sbjct: 181 KSGKVLHSLQYSAMDFDTASKLIKDKQVTVVGLQKSALDLAMECANANGPKNPCTLLFRT 240

Query: 241 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 300
           AHWN+PD HPWGIPL+FLYM+RFAELLIHKPGESFLLYLLA+ILSPIRWLFAKIVETY T
Sbjct: 241 AHWNLPDHHPWGIPLTFLYMSRFAELLIHKPGESFLLYLLAVILSPIRWLFAKIVETYAT 300

Query: 301 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 360
           RK G+AKYGMVPTHGFLQDISSCL+A +PEKFYEKVDEGSIILKKSQSF FCEDGIMI+G
Sbjct: 301 RKHGLAKYGMVPTHGFLQDISSCLLASLPEKFYEKVDEGSIILKKSQSFCFCEDGIMIEG 360

Query: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 420
           ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF DSVIPLYR CIHPRIPQLAVIG
Sbjct: 361 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFNDSVIPLYRQCIHPRIPQLAVIG 420

Query: 421 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 480
           FTESPSNLF SE+RCRWVAEFLDGTFKLPSIKEMEKDIA WEKS+T YSGPF+KRACLAI
Sbjct: 421 FTESPSNLFTSELRCRWVAEFLDGTFKLPSIKEMEKDIANWEKSLTYYSGPFAKRACLAI 480

Query: 481 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDYASP 515
           V+IWY+D LCKDMGWNPMRKKG FANLFVP+GPSDYA+P
Sbjct: 481 VHIWYSDLLCKDMGWNPMRKKGLFANLFVPHGPSDYATP 519

BLAST of CmoCh02G002870 vs. TAIR 10
Match: AT1G19250.1 (flavin-dependent monooxygenase 1 )

HSP 1 Score: 408.7 bits (1049), Expect = 7.0e-114
Identity = 212/516 (41.09%), Postives = 306/516 (59.30%), Query Frame = 0

Query: 5   KVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW------TETLQTPREIYQFSDF 64
           +VAIIGAGVSGL A K  +     P VFEA  ++GGVW      T  LQ+ R  Y+FSDF
Sbjct: 12  RVAIIGAGVSGLAAAKNLVHHN--PTVFEASDSVGGVWRSCTYETTKLQSARVDYEFSDF 71

Query: 65  PWPKSVTE-EFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWG 124
           PWP +  +  FP Y ++LDY++SYA HF L+K ++  ++V+ + + G  D E       G
Sbjct: 72  PWPNNRDDTTFPPYLEILDYLESYAKHFDLLKFMKFGSKVIEVRFIG--DGETPQMVDLG 131

Query: 125 GSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVILCVGRFSDIPNIPEFPPNGGPEAFKS 184
             G     +  W + V    + D  Q    +FV++C G++ D+P IP FP   GPE F+ 
Sbjct: 132 AYGNLLPGKPVWEVAVQIGDSGD-IQWHAFEFVVVCTGKYGDVPRIPAFPAKKGPEMFQ- 191

Query: 185 GKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPK--NPCTVLYRN 244
           GKV+HS+ Y  ++ + AS L+  K+V V+G  K+A+DLA+E A AN  +    CT++ R 
Sbjct: 192 GKVMHSMDYCKLEKEEASTLLSGKKVAVIGFKKSAIDLALESALANQGEGGKACTMVVRT 251

Query: 245 AHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVETYVT 304
            HW IP    WG+P    Y +R ++ L  +P +SFL  L  ++ S +R + +K +E+YV 
Sbjct: 252 THWGIPHYWVWGLPFFLFYSSRASQFLHDRPNQSFLRTLFCLLFSLLRAVVSKFIESYVL 311

Query: 305 RKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGIMIKG 364
            KL + KYG+ P H F +D +SC +AI+PE F+E+ D+G I  KKS  +WF E+GI+ + 
Sbjct: 312 WKLPLEKYGLKPNHSFEEDYASCQMAIIPENFFEEADKGMIRFKKSSKWWFYEEGIVFED 371

Query: 365 ETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQLAVIG 424
            T  + +D+VILATG+ G +KLK I     FR  + F   V+PLYR  IHP IP +  +G
Sbjct: 372 GT-TLEADVVILATGYDGKKKLKAI-VPEPFRTWLEFPSGVMPLYRGTIHPLIPNMGFVG 431

Query: 425 FTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRACLAI 484
           + +S SNL  SE+R  W++  +D  F+LPS ++M     K E  +TR S  F KR C++ 
Sbjct: 432 YVQSSSNLHTSELRSMWLSRLVDEKFRLPSKEKMLDQFLK-EMEVTRNSSRFYKRHCIST 491

Query: 485 VNIWYNDQLCKDMGWNPMRKKGFFANLFVPYGPSDY 512
            +I + D +C DMG NP RK  F    F PYG  DY
Sbjct: 492 FSIQHADDMCNDMGLNPWRKSNFLLEAFSPYGSQDY 518

BLAST of CmoCh02G002870 vs. TAIR 10
Match: AT5G45180.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.9e-90
Identity = 196/501 (39.12%), Postives = 273/501 (54.49%), Query Frame = 0

Query: 5   KVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW------TETLQTPREIYQFSDF 64
           +VAIIGAGVSGL A K        P VFEA  +IGGVW      T  LQ+ R  Y+ SDF
Sbjct: 12  RVAIIGAGVSGLAAAKHLARHH--PQVFEASDSIGGVWRKCTYETTKLQSVRVSYELSDF 71

Query: 65  PWPKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRLNTRVLSIDYEGCSDEELEGSTLWGG 124
            WP      FP Y  VLDY+++YA HF L+K I+ N++V+ + + G      +G TL  G
Sbjct: 72  LWPNRGESSFPTYVDVLDYLEAYAKHFNLVKFIKFNSKVVELRFIG------DGKTLQMG 131

Query: 125 SGEAFGE----RRKWRLNVVDAQTNDPP-QETVVDFVILCVGRFSDIPNIPEFPPNGGPE 184
              A+G     +  W + V    T D   Q    ++V++C G++ D+P  P FP   GPE
Sbjct: 132 DLGAYGNLLPGKPVWEVAV---NTGDGDIQWHAFEYVVVCAGKYGDVPRTPTFPVKKGPE 191

Query: 185 AFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTALDLAMECANANGPK--NPCTV 244
            FK GKVLHS+ YS +  + AS L+  K+V V+G  K+A+DLA+E A AN  K    CT+
Sbjct: 192 IFK-GKVLHSMDYSKLQKEKASQLLHGKKVAVIGFKKSAIDLALESALANQGKEGKTCTM 251

Query: 245 LYRNAHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLLYLLAIILSPIRWLFAKIVE 304
           + R  HW IP  H W                                    R   +K +E
Sbjct: 252 VVRTPHWVIP--HYW------------------------------------RATVSKFIE 311

Query: 305 TYVTRKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVDEGSIILKKSQSFWFCEDGI 364
           +YV  KL + KYG+ P H F +D +SC +A+VPEKF+E+ D+G I  K++ ++WF ++GI
Sbjct: 312 SYVLWKLPLEKYGLKPDHAFEEDYASCQMALVPEKFFEEADKGMIRFKRTTNWWFYDEGI 371

Query: 365 MIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTFKDSVIPLYRHCIHPRIPQL 424
             +  T  + +D+VILATG+ G +KLK I     FR  + F   ++PLYR  IHP IP +
Sbjct: 372 EFEDGT-TLEADVVILATGYDGMKKLKAI-VPEPFRSWLEFPWGIMPLYRGTIHPLIPNM 431

Query: 425 AVIGFTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEMEKDIAKWEKSMTRYSGPFSKRA 484
             IG+ +S SNL +SE+  RW+++ LDG F LPS ++M     K E  + R S  F K  
Sbjct: 432 GFIGYVQSSSNLKSSELHSRWLSQLLDGKFTLPSKEKMLDQFLK-EMHVMRRSSRFFKNH 459

Query: 485 CLAIVNIWYNDQLCKDMGWNP 493
           C +  +I + D L KDM   P
Sbjct: 492 CFSTFSIQHADDLSKDMNLKP 459

BLAST of CmoCh02G002870 vs. TAIR 10
Match: AT1G62570.1 (flavin-monooxygenase glucosinolate S-oxygenase 4 )

HSP 1 Score: 92.0 bits (227), Expect = 1.5e-18
Identity = 104/483 (21.53%), Postives = 174/483 (36.02%), Query Frame = 0

Query: 6   VAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQT----------------- 65
           VA+IGAG +GLVA +    +G   +V +    +GG+W  T +T                 
Sbjct: 13  VAVIGAGAAGLVAARELRREGHTVVVLDREKQVGGLWVYTPETESDELGLDPTRPIVHSS 72

Query: 66  ---------PREIYQFSDFPW------PKSVTEEFPKYNQVLDYIKSYADHFGLMKHIRL 125
                    PRE   + DFP+      P   +  +P + +VL Y++ +A  F + + IR 
Sbjct: 73  VYKSLRTNLPRECMGYKDFPFVPRGDDPSRDSRRYPSHREVLAYLQDFATEFNIEEMIRF 132

Query: 126 NTRVLSIDYEGCSDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDP-PQETVVDFVIL 185
            T VL +       E + G               KWR   V ++T      + + D V++
Sbjct: 133 ETEVLRV-------EPVNG---------------KWR---VQSKTGGGFSNDEIYDAVVM 192

Query: 186 CVGRFSDIPNIPEFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTA 245
           C G F++ PNI + P   G E++  G+  HS  Y   D        KD+ V+V+G   + 
Sbjct: 193 CCGHFAE-PNIAQIP---GIESW-PGRQTHSHSYRVPD------PFKDEVVVVIGNFASG 252

Query: 246 LDLAMECANANGPKNPCTVLYRNAHWNIPDRHPWGIPLSFLYMNRFAELLIHKPGESFLL 305
            D++ + +          +  R +  N  ++ P  +P + L+M                 
Sbjct: 253 ADISRDISKV---AKEVHIASRASKSNTFEKRP--VPNNNLWM----------------- 312

Query: 306 YLLAIILSPIRWLFAKIVETYVTRKLGMAKYGMVPTHGFLQDISSCLIAIVPEKFYEKVD 365
                                                                  + ++D
Sbjct: 313 -------------------------------------------------------HSEID 364

Query: 366 EGSIILKKSQSFWFCEDGIMIKGETKPIRSDLVILATGFRGDQKLKQIFTSATFRDCMTF 425
                          EDG ++    K + +D ++  TG+      K  F      + M  
Sbjct: 373 TAH------------EDGTIVFKNGKVVHADTIVHCTGY------KYYFPFLETNNYMRV 364

Query: 426 KDS-VIPLYRHCIHPRI-PQLAVIGFTESPSNLFASEMRCRWVAEFLDGTFKLPSIKEME 454
            D+ V PLY+H   P + P L+ IG        +  E++ +WVA  L G   LPS+ EM 
Sbjct: 433 DDNRVEPLYKHIFPPALAPGLSFIGLPAMGLQFYMFEVQSKWVAAVLSGRVTLPSVDEMM 364

BLAST of CmoCh02G002870 vs. TAIR 10
Match: AT1G12200.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 81.6 bits (200), Expect = 2.0e-15
Identity = 65/254 (25.59%), Postives = 104/254 (40.94%), Query Frame = 0

Query: 4   KKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVWTETLQT--------------- 63
           + VA+IGAG +GLVA +    +G   +V E    IGGVW  T Q                
Sbjct: 12  RHVAVIGAGAAGLVAARELRREGHSVVVLERGSQIGGVWAYTSQVEPDPLSLDPTRPVVH 71

Query: 64  -----------PREIYQFSDFPWPKSVTE------EFPKYNQVLDYIKSYADHFGLMKHI 123
                      PRE   F+DFP+     +        P + +VL Y++ +A  F + + +
Sbjct: 72  SSLYRSLRTNIPRECMGFTDFPFATRPHDGSRDPRRHPAHTEVLAYLRDFAKEFDIEEMV 131

Query: 124 RLNTRVLSIDYEGCSDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVI 183
           R  T V+  +      E                ER KWR   V+++++D   + + D V+
Sbjct: 132 RFETEVVKAEQVAAEGE----------------ERGKWR---VESRSSDGVVDEIYDAVV 191

Query: 184 LCVGRFSD-----IPNIPEFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVV 221
           +C G +++     I  I  +P          GK +HS  Y   D        KD+ VIV+
Sbjct: 192 VCNGHYTEPRHALITGIDSWP----------GKQIHSHNYRVPD------QFKDQVVIVI 230

BLAST of CmoCh02G002870 vs. TAIR 10
Match: AT5G61290.1 (Flavin-binding monooxygenase family protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.1e-13
Identity = 67/246 (27.24%), Postives = 108/246 (43.90%), Query Frame = 0

Query: 2   EPKKVAIIGAGVSGLVACKFTLSKGFIPIVFEARGAIGGVW--------------TETLQ 61
           + K V +IGAG SGLV+ +    +G   +V E    +GG W              T+TL+
Sbjct: 12  QSKTVCVIGAGPSGLVSARELKKEGHKVVVMEQNHDVGGQWLYQPNVDEEDTLGKTKTLK 71

Query: 62  ------------TPREIYQFSDFPWPKSV---TEEFPKYNQVLDYIKSYADHFGLMKHIR 121
                       +PRE+  FSDFP+       +  FP + ++L Y+K +   FGL + IR
Sbjct: 72  VHSSVYSSLRLASPREVMGFSDFPFIAKEGRDSRRFPGHEELLLYLKDFCQVFGLREMIR 131

Query: 122 LNTRVLSIDYEGCSDEELEGSTLWGGSGEAFGERRKWRLNVVDAQTNDPPQETVVDFVIL 181
            N RV   ++ G  +E+ +             + +KW +  V  + +    E V D V++
Sbjct: 132 FNVRV---EFVGMVNEDDDDD----------DDVKKWMVKSV--KKSGEVMEEVFDAVVV 191

Query: 182 CVGRFSDIPNIPEFPPNGGPEAFKSGKVLHSLQYSAMDFDTASNLIKDKQVIVVGLHKTA 219
             G +S     P  P   G + +K  K LHS  Y   +         D+ V+VVG   + 
Sbjct: 192 ASGHYS----YPRLPTIKGMDLWKR-KQLHSHIYRVPE------PFCDEVVVVVGCSMSG 231

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMA19.9e-11341.09Probable flavin-containing monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=FM... [more]
Q9FKE72.6e-8939.12Putative flavin-containing monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=FM... [more]
Q017409.1e-3426.74Dimethylaniline monooxygenase [N-oxide-forming] 1 OS=Homo sapiens OX=9606 GN=FMO... [more]
Q5REK01.6e-3326.91Dimethylaniline monooxygenase [N-oxide-forming] 2 OS=Pongo abelii OX=9601 GN=FMO... [more]
Q995182.0e-3326.72Dimethylaniline monooxygenase [N-oxide-forming] 2 OS=Homo sapiens OX=9606 GN=FMO... [more]
Match NameE-valueIdentityDescription
A0A6J1G8770.0e+00100.00Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451755 PE... [more]
A0A6J1I4G07.2e-28489.21Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469790 PE=3... [more]
A0A6J1G8Q62.6e-28188.25Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451753 PE... [more]
A0A6J1I2231.6e-26783.43Flavin-containing monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111469791 PE=3... [more]
A0A6J1G8N44.7e-26783.43Flavin-containing monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111451754 PE... [more]
Match NameE-valueIdentityDescription
XP_022948061.10.0e+00100.00probable flavin-containing monooxygenase 1 [Cucurbita moschata][more]
XP_022970985.11.5e-28389.21probable flavin-containing monooxygenase 1 [Cucurbita maxima][more]
XP_022948059.15.3e-28188.25probable flavin-containing monooxygenase 1 [Cucurbita moschata][more]
XP_023534050.15.3e-28188.44probable flavin-containing monooxygenase 1 [Cucurbita pepo subsp. pepo][more]
KAG6604966.19.1e-28188.44putative flavin-containing monooxygenase 1, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
AT1G19250.17.0e-11441.09flavin-dependent monooxygenase 1 [more]
AT5G45180.11.9e-9039.12Flavin-binding monooxygenase family protein [more]
AT1G62570.11.5e-1821.53flavin-monooxygenase glucosinolate S-oxygenase 4 [more]
AT1G12200.12.0e-1525.59Flavin-binding monooxygenase family protein [more]
AT5G61290.11.1e-1327.24Flavin-binding monooxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020946Flavin monooxygenase-likePFAMPF00743FMO-likecoord: 4..492
e-value: 5.3E-38
score: 130.7
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 171..251
e-value: 5.9E-8
score: 35.1
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 3..170
e-value: 1.5E-31
score: 111.6
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 341..503
e-value: 4.3E-13
score: 51.0
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 349..511
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 1..274
IPR000960Flavin monooxygenase FMOPIRSFPIRSF000332FMOcoord: 2..503
e-value: 6.6E-44
score: 147.9
NoneNo IPR availablePANTHERPTHR23023DIMETHYLANILINE MONOOXYGENASEcoord: 4..512
NoneNo IPR availablePANTHERPTHR23023:SF217FLAVIN-CONTAINING MONOOXYGENASEcoord: 4..512
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..19
score: 6.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G002870.1CmoCh02G002870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0004499 N,N-dimethylaniline monooxygenase activity