CmoCh02G002480 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G002480
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionsubtilisin-like protease SBT1.6
LocationCmo_Chr02: 1173478 .. 1175802 (+)
RNA-Seq ExpressionCmoCh02G002480
SyntenyCmoCh02G002480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTTGTTTCGACGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGAAATTTTCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCCAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACCGGTTTAGACTTTGATACACGAAAAACAGAGTTCAATATCTTATCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGACAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCAACACCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAGCGAAGATGATCCAGGTCATATCTCGAACGTCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCTGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAGCCGTCAAAACTGGCATTCTCAACAGCGGTGAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAACTACCCGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

mRNA sequence

ATGGCCGCTTGTTTCGACGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGAAATTTTCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCCAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACCGGTTTAGACTTTGATACACGAAAAACAGAGTTCAATATCTTATCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGACAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCAACACCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAGCGAAGATGATCCAGGTCATATCTCGAACGTCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCTGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAGCCGTCAAAACTGGCATTCTCAACAGCGGTGAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAACTACCCGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

Coding sequence (CDS)

ATGGCCGCTTGTTTCGACGCTTCTCTCTCCTCTTCCATCTTCTTCTCTCTCCTCCTGTTAATCCATGGCTCTACAGTTCCGGGTGATCCGCCATTGAAGACCTTCATCTTCCGTGTGGACAGTTTCTCTAAACCCTCTGTTTTTCCCACCCATTTCCACTGGTACACTTCCGAGTTCGCCGAATCCCACAAAATCCTCCATGTTTACGACACCGTGTTTCATGGATTCTCTGCAACTTTCACTCAAGAGCAAGCCGATTCCGTCGGAAAACACCCTTCTGTGCTTGCTGTCTTTGAGGACCGTCGCCGTCAGCTTCACACCACGCGTTCCCCTCAATTTCTCGGCCTTCGAAACCAGCGTGGTTTGTGGTCTGATTCCGATTATGGGTCGGATGTAATCATTGGAGTTTTCGATACTGGGATTTCCCCTGAACGGCGGAGCTTCTCCGATGTGAATTTGGGGCCGATTCCTCGCCGGTGGAAAGGGGTTTGTCAGACTGGAACTAAGTTCACGGCCAAAAACTGTAATAGGAAGATCGTCGGTGCGAGGTTTTTCTCTAAGGGTCACGAAGCAGGGGCCAACGCTGCTGGTCCGATTATTGGGATTAACGACACCGTTGAGTACCGATCTCCGAGAGACGCCGACGGCCATGGGACTCACACGGCATCGACGGCGGCTGGGCGTCACGCGTTTCAGGCGAGTTTGGAGGGTTATGCTTCGGGAATCGCGAAGGGTGTAGCCCCTAAGGCACGTTTGGCGGTTTACAAAGTTTGTTGGAAAAATTCGGGTTGTTTCGATTCCGATATTCTCGCGGCGTTTGATTCTGCTGTTAAGGACGGCGTCGATGTCATTTCGATCTCGATTGGTGGCGGCGATGGCGTTTCCACGCCGTATTATCTTGACCCAATTGCAATCGGGGCGTACGGCGCCGCTTCTAGGGGCATTTTCGTTTCGTCTTCGGCTGGCAATGATGGACCCAATGGGATGTCAGTGACGAACTTAGCGCCGTGGATTACGACGGTCGGAGCTGGTACGATTGACCGAAATTTTCCGGCGGTGGTGATTATGGGAAACCGACGGAAGATTCACGGCGTTTCTCTGTACGCCGGAGCGCCATTGAACAGTACAATGTATCCGTTGGTTTACCCTGGAAAATCAGGGGAGCTCTCTGCTTCGCTCTGTATGGAGAGTTCGCTTGACCCTAAGATTGTGACCGGAAAAATTGTTATCTGCGACAGAGGAAGTAGCCCGAGAGTGGAGAAAGGTTTGGTGGTGAAGAAGGCCGGCGGCGTCGGAATGATTCTGGCGAATGGAATTTCAAACGGCGAAGGACTCGTCGGTGATGCCCATATCCTTCCCGCCTGCGCCGTTGGCTCTGTAGAAGGCGATGCCATGAAAGCCTACGCATCGTCCTCTGGAAATCCCACCGCCACCATCGCCTTCCGAGGCACACGAATCGGAATCAAACCGGCGCCGGTGGTGGCTTCGTTTTCTGCCAGAGGGCCAAATGGATTAAACCCTGAAATTCTAAAACCGGACATGATCGCACCAGGGGTTAACATTTTAGCCGCTTGGACAGACGTCGTCGGCCCAACCGGTTTAGACTTTGATACACGAAAAACAGAGTTCAATATCTTATCAGGTACATCAATGGCGTGTCCTCATATAAGTGGAGCCGCCGCTCTGTTGAAATCAGCACATCCCGATTGGAGTCCGGCGGCGGTAAGATCCGCCATGATGACCACCGCAAGAATCACTGACAACCGACGACAGCCGACGACGGAAGAATCCACCGGAAAAGCTTCAACACCGTACGATTTCGGTGCCGGTCATGTGAATCTCGGCCTTGCAATGGACCCTGGACTAGTCTACGACATTACAAACACCGATTATGTCAACTTCCTATGCTCAATCGGCTACGGAGCGAAGATGATCCAGGTCATATCTCGAACGTCGGTGAAATGTCCGGCGAGGAAGCCATTGCCAGAGAATTTGAACTACCCATCAATCGTAGCTGTGTTTTCCAGTCTATCAAAAGGGTTGTCCACAAAATCGTTTATCCGAACGGCGACGAACGTGGGGGCGGCGAATTCGGTGTACCAAGCTAAAATCGAAGCTCCGAAAGGGGCGACGGTGAAGGTAAAGCCGTCAAAACTGGCATTCTCAACAGCGGTGAAGAAACAGAGCTTCGTAGTGGCCATATCAGCCAACTACCCGAATCTGACACTAGGCGATGTGGGTGCAGTCTTTGGTTCTCTTTCTTGGTCTGATGGAAAGCATGTGGTCCGAACACCACTGGTAGTTACTAAATTAGAGCCGTTGTAG

Protein sequence

MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLAFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
Homology
[Close]
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 554/767 (72.23%), Postives = 655/767 (85.40%), Query Frame = 0 

Query: 10  SSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +       KTFIFR+D  S PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV+DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAG 429
           G PLN  M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRV KGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCP-ARKPLPENLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RT V+CP  RKP P NLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLAFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +L F++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANYPNLTLGDVGAVFGSLSWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N  N+ LG+ GAVFGS++W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

[Close]
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 3.1e-243
Identity = 443/779 (56.87%), Postives = 545/779 (69.96%), Query Frame = 0 

Query: 14  FFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA----ESHKILHVY 73
           FF LL L   S+        T+I  VD  +KPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 133
           DTVFHGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-STMYPLVYPGK---SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGL 433
           Y G  L+   MYPLVY G        S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEES 613
            R+TEFNILSGTSMACPH+SG AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPA 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 RKPLPE--NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 733
            +      NLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLAFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           P KL+F    +K SFVV +      L+ G      G + WSDGK  V +PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

[Close]
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 5.5e-208
Identity = 390/770 (50.65%), Postives = 522/770 (67.79%), Query Frame = 0 

Query: 9   LSSSIFFSLLLL--IHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+   D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNSTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV+K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR +  C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 AFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + +    +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

[Close]
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.4e-206
Identity = 382/769 (49.67%), Postives = 510/769 (66.32%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MA+   +S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADS-VGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T FHGFSA     +ADS +    S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  + +    LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 480
           EKG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 600
           +T+FNI+SGTSM+CPHISG A LLK+AHP+WSP+A++SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGA-KMIQVISRTSVKCPAR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R SV C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSK 720
              P  LNYPS    FS L  G     + R  TNVGAA+SVY+  +       + VKPSK
Sbjct: 661 FSDPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LAF-STAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPL 767
           L+F S   KK+  V  +S    ++T     A FGS++WS+ +H VR+P+
Sbjct: 721 LSFKSVGEKKRYTVTFVSKKGVSMT---NKAEFGSITWSNPQHEVRSPV 747

[Close]
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 5.7e-197
Identity = 373/777 (48.01%), Postives = 498/777 (64.09%), Query Frame = 0 

Query: 18  LLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  + P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NSTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +   +  YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
           +KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKC--PA 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V ++ S +     
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 RKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPS 737
               P NLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+P 
Sbjct: 680 LAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 738 KLAFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
            L F++  +K S+ V     +           FG L W    H VR+P+++T L PL
Sbjct: 740 TLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

[Close]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match: A0A6J1G574 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111450975 PE=3 SV=1)

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 774/774 (100.00%), Postives = 774/774 (100.00%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match: A0A6J1I5D0 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111469756 PE=3 SV=1)

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 748/774 (96.64%), Postives = 753/774 (97.29%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACF  SLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVD F+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
            EFNILSGTSMACPHISGAAALLKSAH DWSPAA+RSAMMTTA+ITDNR QPTTEESTGK
Sbjct: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRT VKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LP NLNYPSIVAVFSSLSK LSTKSFIRTATNVGAANSVYQAKIEAPKG TVKVKPSKL 
Sbjct: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN  NLTLG+VGAVFGSLSWSDGKHVVR PLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match: A0A6J1JEF5 (subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3 SV=1)

HSP 1 Score: 1382.5 bits (3577), Expect = 0.0e+00
Identity = 688/769 (89.47%), Postives = 726/769 (94.41%), Query Frame = 0 

Query: 7   ASLSSSIFFSLLLLIHGSTVPGDPPL-KTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKI 66
           A+  S +FF  LL++H  TV  D PL KTFIFRVD FSKPSVFPTH+HWYTSEFAESHKI
Sbjct: 2   AAYLSPLFFFFLLIVHRLTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAESHKI 61

Query: 67  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 126
           LHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD
Sbjct: 62  LHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSD 121

Query: 127 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFF 186
           SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVGARFF
Sbjct: 122 SDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVGARFF 181

Query: 187 SKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKG 246
           SKGHEAG+NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASGIAKG
Sbjct: 182 SKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASGIAKG 241

Query: 247 VAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAI 306
           VAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYLDPIAI
Sbjct: 242 VAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLDPIAI 301

Query: 307 GAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGV 366
           GAYGAASRGIFVSSS GNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+I GV
Sbjct: 302 GAYGAASRGIFVSSSGGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRRISGV 361

Query: 367 SLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVV 426
           SLYAGAPLN+TM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRV KGLVV
Sbjct: 362 SLYAGAPLNATMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAKGLVV 421

Query: 427 KKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTR 486
           KKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF+GT 
Sbjct: 422 KKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSANPTATIAFQGTI 481

Query: 487 IGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNI 546
           IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKTEFNI
Sbjct: 482 IGIKPAPVVASFSARGPNGLNPEILKPDLIAPGVNILAAWTDAVGPTGLDFDTRKTEFNI 541

Query: 547 LSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPY 606
           LSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKASTPY
Sbjct: 542 LSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKASTPY 601

Query: 607 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKPLPENL 666
           DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVI+RT V CPA+KPLPENL
Sbjct: 602 DFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGPKMIQVITRTPVTCPAKKPLPENL 661

Query: 667 NYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLAFSTAV 726
           NYPSIVAVFSSLSKG STKSFIRT TNVG ANSVY+AKIEAPKG TVKVKPSKL FS A+
Sbjct: 662 NYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVFSAAM 721

Query: 727 KKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           KKQSFVVA+SA+  NL LGDVGAVFG +SWSDGKHVVR+PLVVT+LEPL
Sbjct: 722 KKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 770

[Close]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match: A0A6J1FYE5 (subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE=3 SV=1)

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 688/773 (89.00%), Postives = 725/773 (93.79%), Query Frame = 0 

Query: 3   ACFDASLSSSIFFSLLLLIHGSTVPGDPPL-KTFIFRVDSFSKPSVFPTHFHWYTSEFAE 62
           A + + L    FF  LL++H  TV  D PL KTFIFRVD FSKPSVFPTH+HWYTSEFAE
Sbjct: 2   AAYLSPLFFFFFFFFLLIVHRFTVSADSPLKKTFIFRVDRFSKPSVFPTHYHWYTSEFAE 61

Query: 63  SHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 122
           SHKILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG
Sbjct: 62  SHKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG 121

Query: 123 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVG 182
           LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTA+NCNRKIVG
Sbjct: 122 LWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTARNCNRKIVG 181

Query: 183 ARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASG 242
           ARFFSKGHEAG+NAAGPIIGINDT+E+RSPRDADGHGTHTASTAAGRHAFQASLEG+ASG
Sbjct: 182 ARFFSKGHEAGSNAAGPIIGINDTIEFRSPRDADGHGTHTASTAAGRHAFQASLEGFASG 241

Query: 243 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLD 302
           IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYLD
Sbjct: 242 IAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYLD 301

Query: 303 PIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRK 362
           PIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R+
Sbjct: 302 PIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGRR 361

Query: 363 IHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEK 422
           I GVSLYAGAPLN TM+PLVYPGKSG LS SLCM++SLDPK+V GKIVICDRGSSPRV K
Sbjct: 362 ISGVSLYAGAPLNGTMFPLVYPGKSGVLSVSLCMDNSLDPKVVAGKIVICDRGSSPRVAK 421

Query: 423 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 482
           GLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGD+MKAYASSS NPTATIAF
Sbjct: 422 GLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDSMKAYASSSPNPTATIAF 481

Query: 483 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 542
           +GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRKT
Sbjct: 482 QGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRKT 541

Query: 543 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 602
           EFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA  TDNRRQP TEESTGKA
Sbjct: 542 EFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASTTDNRRQPMTEESTGKA 601

Query: 603 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKPL 662
           STPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVI+RT V CPA+KPL
Sbjct: 602 STPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVTCPAKKPL 661

Query: 663 PENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLAF 722
           PENLNYPSIVAVFSSLSKG STKSFIRT TNVG ANSVY+AKIEAPKG TVKVKPSKL F
Sbjct: 662 PENLNYPSIVAVFSSLSKGWSTKSFIRTVTNVGPANSVYRAKIEAPKGVTVKVKPSKLVF 721

Query: 723 STAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           S A KKQSFVVA+SA+  NL LGDVGAVFG +SWSDGKHVVR+PLVVT+LEPL
Sbjct: 722 SAATKKQSFVVAVSADNQNLALGDVGAVFGWVSWSDGKHVVRSPLVVTQLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match: A0A0A0LJ58 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1)

HSP 1 Score: 1378.6 bits (3567), Expect = 0.0e+00
Identity = 690/774 (89.15%), Postives = 726/774 (93.80%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAA    SL SS FFS LLL+  STV    PLKTFI R+D FSKPSVFPTH+HWYTSEF 
Sbjct: 1   MAAFLSTSL-SSFFFSFLLLL--STVSSHSPLKTFIVRIDRFSKPSVFPTHYHWYTSEFT 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           +S +ILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  QSPQILHVYDTVFHGFSATLTQDQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRH+FQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHSFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIG+YGAAS+G+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFP+VV +GN R
Sbjct: 301 DPIAIGSYGAASKGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPSVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KI+GVSLYAGAPLN TMYPLVYPGKSG LS SLCME+SLDPK+VTGKIVICDRGSSPRV 
Sbjct: 361 KIYGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKVVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFD RK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDKRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA ITDNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
            STPYDFGAGHVNLGLAMDPGL+YDITNTDY+NFLCSIGYG KMIQVI+RT V+CP +KP
Sbjct: 601 PSTPYDFGAGHVNLGLAMDPGLIYDITNTDYINFLCSIGYGPKMIQVITRTPVRCPTKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LPENLNYPSIV VFSSLSKG STKSFIRTATNVG +NSVY+ KIEAPKG TVKVKPSKL 
Sbjct: 661 LPENLNYPSIVTVFSSLSKGWSTKSFIRTATNVGPSNSVYRVKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FST VKKQSFVVAISA+  NL LGDVGAVFG LSWSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 771

[Close]
BLAST of CmoCh02G002480 vs. NCBI nr
Match: XP_022946948.1 (subtilisin-like protease SBT1.6 [Cucurbita moschata])

HSP 1 Score: 1535.0 bits (3973), Expect = 0.0e+00
Identity = 774/774 (100.00%), Postives = 774/774 (100.00%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. NCBI nr
Match: KAG6604929.1 (Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027008.1 Subtilisin-like protease SBT1.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1520.0 bits (3934), Expect = 0.0e+00
Identity = 768/774 (99.22%), Postives = 768/774 (99.22%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACF ASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFAASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE
Sbjct: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYG KMIQVISRT VKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGTKMIQVISRTPVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKL 
Sbjct: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN  NLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANDQNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. NCBI nr
Match: XP_023532451.1 (subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1499.2 bits (3880), Expect = 0.0e+00
Identity = 758/774 (97.93%), Postives = 760/774 (98.19%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACF ASLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVDSF+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFAASLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDSFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTV HGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVVHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK
Sbjct: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGY +KMIQVISRT VKCP RKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYRSKMIQVISRTPVKCPTRKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LP NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKL 
Sbjct: 661 LPGNLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN  NLTLGDVGAVFGSL WSDGKHVVRTPLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGDVGAVFGSLLWSDGKHVVRTPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. NCBI nr
Match: XP_022970938.1 (subtilisin-like protease SBT1.6 [Cucurbita maxima])

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 748/774 (96.64%), Postives = 753/774 (97.29%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAACF  SLSSSIFFSLLLLIHGSTVP DPPLKTFIFRVD F+KPSVFPTHFHWYTSEFA
Sbjct: 1   MAACFATSLSSSIFFSLLLLIHGSTVPADPPLKTFIFRVDRFAKPSVFPTHFHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGI PERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGICPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NA GPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNADGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAV DGVDVISISIGGGDGVSTPYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVNDGVDVISISIGGGDGVSTPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR
Sbjct: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KIHGVSLYAGAPLN TMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 
Sbjct: 361 KIHGVSLYAGAPLNGTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSS NPTATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSRNPTATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK
Sbjct: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
            EFNILSGTSMACPHISGAAALLKSAH DWSPAA+RSAMMTTA+ITDNR QPTTEESTGK
Sbjct: 541 IEFNILSGTSMACPHISGAAALLKSAHLDWSPAAIRSAMMTTAKITDNRWQPTTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRT VKCPARKP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTPVKCPARKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LP NLNYPSIVAVFSSLSK LSTKSFIRTATNVGAANSVYQAKIEAPKG TVKVKPSKL 
Sbjct: 661 LPGNLNYPSIVAVFSSLSKVLSTKSFIRTATNVGAANSVYQAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FSTAVKKQSFVVAISAN  NLTLG+VGAVFGSLSWSDGKHVVR PLVVTKLEPL
Sbjct: 721 FSTAVKKQSFVVAISANNQNLTLGNVGAVFGSLSWSDGKHVVRMPLVVTKLEPL 774

[Close]
BLAST of CmoCh02G002480 vs. NCBI nr
Match: XP_038901136.1 (subtilisin-like protease SBT1.6 [Benincasa hispida])

HSP 1 Score: 1390.6 bits (3598), Expect = 0.0e+00
Identity = 700/774 (90.44%), Postives = 726/774 (93.80%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MAA    SLS   FF LLLL   STV  D PLKTFIFRVD FSKPSVFPTH+HWYTSEFA
Sbjct: 1   MAAFLSTSLSP--FFFLLLLTLHSTVSADSPLKTFIFRVDRFSKPSVFPTHYHWYTSEFA 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120
           ES KILHVYDTVFHGFSAT TQ+Q DS+GKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR
Sbjct: 61  ESPKILHVYDTVFHGFSATLTQQQVDSIGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQR 120

Query: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIV 180
           GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVC+TGTKFTAKNCNRKIV
Sbjct: 121 GLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCETGTKFTAKNCNRKIV 180

Query: 181 GARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240
           GARFFSKGHEAG+NAAGPIIGINDT+EYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS
Sbjct: 181 GARFFSKGHEAGSNAAGPIIGINDTIEYRSPRDADGHGTHTASTAAGRHAFQASLEGYAS 240

Query: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYL 300
           GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFD+AV DGVDVISISIGGGDGVS+PYYL
Sbjct: 241 GIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDAAVNDGVDVISISIGGGDGVSSPYYL 300

Query: 301 DPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRR 360
           DPIAIGAYGAASRG+FVSSSAGNDGPNGMSVTNLAPW+TTVGAGTIDRNFPAVV +GN R
Sbjct: 301 DPIAIGAYGAASRGVFVSSSAGNDGPNGMSVTNLAPWVTTVGAGTIDRNFPAVVTLGNGR 360

Query: 361 KIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVE 420
           KI GVSLYAGAPLN TMYPLVYPGKSG LS SLCME+SLDPK V GKIVICDRGSSPRV 
Sbjct: 361 KISGVSLYAGAPLNGTMYPLVYPGKSGVLSVSLCMENSLDPKAVAGKIVICDRGSSPRVA 420

Query: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIA 480
           KGLVVKKAGGVGMILANGISNGEGLVGDAH+LPACAVGS EGDAMKAYASSS NP ATIA
Sbjct: 421 KGLVVKKAGGVGMILANGISNGEGLVGDAHLLPACAVGSDEGDAMKAYASSSTNPIATIA 480

Query: 481 FRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRK 540
           F+GT IGIKPAPVVASFSARGPNGLNPEILKPD+IAPGVNILAAWTD VGPTGLDFDTRK
Sbjct: 481 FQGTIIGIKPAPVVASFSARGPNGLNPEILKPDIIAPGVNILAAWTDAVGPTGLDFDTRK 540

Query: 541 TEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGK 600
           TEFNILSGTSMACPH+SGAAALLKSAHPDWSPAA+RSAMMTTA ITDNRRQP TEESTGK
Sbjct: 541 TEFNILSGTSMACPHVSGAAALLKSAHPDWSPAALRSAMMTTASITDNRRQPMTEESTGK 600

Query: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPARKP 660
           ASTPYDFGAGHVNLGLAMDPGLVYDITNTDY+NFLCSIGYG KMIQVI+RT VKCPA+KP
Sbjct: 601 ASTPYDFGAGHVNLGLAMDPGLVYDITNTDYINFLCSIGYGPKMIQVITRTPVKCPAKKP 660

Query: 661 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLA 720
           LPENLNYPSIV+VFSSLSKG STKSFIRT TNVG +NS Y+AKIEAPKG TVKVKPSKL 
Sbjct: 661 LPENLNYPSIVSVFSSLSKGWSTKSFIRTVTNVGPSNSAYRAKIEAPKGVTVKVKPSKLV 720

Query: 721 FSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           FST VKKQSFVVAISA+  NL LGDVGAVFG LSWSDGKHVVR+PLVVT+LEPL
Sbjct: 721 FSTTVKKQSFVVAISADNQNLALGDVGAVFGWLSWSDGKHVVRSPLVVTQLEPL 772

[Close]
BLAST of CmoCh02G002480 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 1136.3 bits (2938), Expect = 0.0e+00
Identity = 554/767 (72.23%), Postives = 655/767 (85.40%), Query Frame = 0 

Query: 10  SSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFAESHKILHVY 69
           SS+I   L L     +       KTFIFR+D  S PS+FPTH+HWY++EFAE  +I+HVY
Sbjct: 3   SSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEESRIVHVY 62

Query: 70  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRGLWSDSDYG 129
            TVFHGFSA  T ++AD++  HP+VLAVFEDRRR+LHTTRSPQFLGL+NQ+GLWS+SDYG
Sbjct: 63  HTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYG 122

Query: 130 SDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSKGH 189
           SDVIIGVFDTGI PERRSFSD+NLGPIP+RW+GVC++G +F+ +NCNRKI+GARFF+KG 
Sbjct: 123 SDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQ 182

Query: 190 EAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVAPK 249
           +A       I GIN TVE+ SPRDADGHGTHT+STAAGRHAF+AS+ GYASG+AKGVAPK
Sbjct: 183 QAAV-----IGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPK 242

Query: 250 ARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGAYG 309
           AR+A YKVCWK+SGC DSDILAAFD+AV+DGVDVISISIGGGDG+++PYYLDPIAIG+YG
Sbjct: 243 ARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYG 302

Query: 310 AASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSLYA 369
           AAS+GIFVSSSAGN+GPNGMSVTNLAPW+TTVGA TIDRNFPA  I+G+  ++ GVSLYA
Sbjct: 303 AASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYA 362

Query: 370 GAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGLVVKKAG 429
           G PLN  M+P+VYPGKSG  SASLCME++LDPK V GKIVICDRGSSPRV KGLVVKKAG
Sbjct: 363 GVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAG 422

Query: 430 GVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAFRGTRIGIK 489
           GVGMILANG SNGEGLVGDAH++PACAVGS EGD +KAYASS  NP A+I FRGT +GIK
Sbjct: 423 GVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIK 482

Query: 490 PAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKTEFNILSGT 549
           PAPV+ASFS RGPNGL+PEILKPD+IAPGVNILAAWTD VGPTGL  D RKTEFNILSGT
Sbjct: 483 PAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGT 542

Query: 550 SMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKASTPYDFGA 609
           SMACPH+SGAAALLKSAHPDWSPA +RSAMMTT  + DN  +   +ESTGK++TPYD+G+
Sbjct: 543 SMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGS 602

Query: 610 GHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCP-ARKPLPENLNYP 669
           GH+NLG AM+PGLVYDITN DY+ FLCSIGYG K IQVI+RT V+CP  RKP P NLNYP
Sbjct: 603 GHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYP 662

Query: 670 SIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSKLAFSTAVKKQ 729
           SI AVF +  +GL +K+ IRTATNVG A +VY+A+IE+P+G TV VKP +L F++AVK++
Sbjct: 663 SITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRR 722

Query: 730 SFVVAISANYPNLTLGDVGAVFGSLSWSD-GKHVVRTPLVVTKLEPL 775
           S+ V ++ N  N+ LG+ GAVFGS++W D GKHVVR+P+VVT+++ L
Sbjct: 723 SYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVTQMDTL 764

[Close]
BLAST of CmoCh02G002480 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 842.8 bits (2176), Expect = 2.2e-244
Identity = 443/779 (56.87%), Postives = 545/779 (69.96%), Query Frame = 0 

Query: 14  FFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA----ESHKILHVY 73
           FF LL L   S+        T+I  VD  +KPS+FPTHFHWYTS  A        I+H Y
Sbjct: 7   FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTY 66

Query: 74  DTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLR--NQRGLWSDSD 133
           DTVFHGFSA  T + A  +  HP V++V  ++ R LHTTRSP+FLGLR  ++ GL  +SD
Sbjct: 67  DTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESD 126

Query: 134 YGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGARFFSK 193
           +GSD++IGV DTG+ PER SF D  LGP+P +WKG C     F    CNRK+VGARFF  
Sbjct: 127 FGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCG 186

Query: 194 GHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIAKGVA 253
           G+EA          +N+T E+RSPRD+DGHGTHTAS +AGR+ F AS  GYA G+A G+A
Sbjct: 187 GYEATNGK------MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 246

Query: 254 PKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPIAIGA 313
           PKARLA YKVCW NSGC+DSDILAAFD+AV DGVDVIS+S+G   GV  PYYLD IAIGA
Sbjct: 247 PKARLAAYKVCW-NSGCYDSDILAAFDTAVADGVDVISLSVG---GVVVPYYLDAIAIGA 306

Query: 314 YGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIHGVSL 373
           +GA  RGIFVS+SAGN GP  ++VTN+APW+TTVGAGTIDR+FPA V +GN + I GVS+
Sbjct: 307 FGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSV 366

Query: 374 YAGAPLN-STMYPLVYPGK---SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEKGL 433
           Y G  L+   MYPLVY G        S+SLC+E SLDP +V GKIV+CDRG + R  KG 
Sbjct: 367 YGGPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGE 426

Query: 434 VVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYAS------SSGNPTA 493
           +V+K GG+GMI+ANG+ +GEGLV D H+LPA +VG+  GD ++ Y S      SS +PTA
Sbjct: 427 IVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTA 486

Query: 494 TIAFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFD 553
           TI F+GTR+GI+PAPVVASFSARGPN   PEILKPD+IAPG+NILAAW D +GP+G+  D
Sbjct: 487 TIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSD 546

Query: 554 TRKTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEES 613
            R+TEFNILSGTSMACPH+SG AALLK+AHPDWSPAA+RSA++TTA   DN  +P  +ES
Sbjct: 547 NRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDES 606

Query: 614 TGKASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKCPA 673
           TG  S+  D+G+GHV+   AMDPGLVYDIT+ DY+NFLC+  Y    I  I+R    C  
Sbjct: 607 TGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDG 666

Query: 674 RKPLPE--NLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVK 733
            +      NLNYPS   VF    +   +  FIRT TNVG ++SVY+ KI  P+G TV V+
Sbjct: 667 ARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVE 726

Query: 734 PSKLAFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
           P KL+F    +K SFVV +      L+ G      G + WSDGK  V +PLVVT  +PL
Sbjct: 727 PEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVTLQQPL 775

[Close]
BLAST of CmoCh02G002480 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 725.7 bits (1872), Expect = 3.9e-209
Identity = 390/770 (50.65%), Postives = 522/770 (67.79%), Query Frame = 0 

Query: 9   LSSSIFFSLLLL--IHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS---EFAESH 68
           LSS+ FF LL L   H S+   D    T+I  +     PS F  H +WY S     ++S 
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQ--GTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA 65

Query: 69  KILHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGL-RNQRGL 128
           ++L+ Y+   HGFS   TQE+ADS+   P V++V  + R +LHTTR+P FLGL  +   L
Sbjct: 66  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 125

Query: 129 WSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGA 188
           + ++   SDV++GV DTG+ PE +S+SD   GPIP  WKG C+ GT FTA  CNRK++GA
Sbjct: 126 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 185

Query: 189 RFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGI 248
           RFF++G+E   +  GP   I+++ E RSPRD DGHGTHT+STAAG     ASL GYASG 
Sbjct: 186 RFFARGYE---STMGP---IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 245

Query: 249 AKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDP 308
           A+G+AP+AR+AVYKVCW   GCF SDILAA D A+ D V+V+S+S+GGG    + YY D 
Sbjct: 246 ARGMAPRARVAVYKVCWL-GGCFSSDILAAIDKAIADNVNVLSMSLGGG---MSDYYRDG 305

Query: 309 IAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKI 368
           +AIGA+ A  RGI VS SAGN GP+  S++N+APWITTVGAGT+DR+FPA+ I+GN +  
Sbjct: 306 VAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNF 365

Query: 369 HGVSLYAGAPLNSTMYPLVYPGK-SGELSASLCMESSLDPKIVTGKIVICDRGSSPRVEK 428
            GVSL+ G  L   + P +Y G  S   + +LCM  +L P+ V GKIV+CDRG + RV+K
Sbjct: 366 TGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQK 425

Query: 429 GLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATIAF 488
           G VVK AGGVGMILAN  +NGE LV DAH+LPA  VG   GD ++ Y ++  NPTA+I+ 
Sbjct: 426 GDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISI 485

Query: 489 RGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTRKT 548
            GT +G+KP+PVVA+FS+RGPN + P ILKPD+IAPGVNILAAWT   GPTGL  D+R+ 
Sbjct: 486 LGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 545

Query: 549 EFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTGKA 608
           EFNI+SGTSM+CPH+SG AALLKS HP+WSPAA+RSA+MTTA  T    +P  + +TGK 
Sbjct: 546 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKP 605

Query: 609 STPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKC-PARKP 668
           STP+D GAGHV+   A +PGL+YD+T  DY+ FLC++ Y +  I+ +SR +  C P++  
Sbjct: 606 STPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSY 665

Query: 669 LPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEA-PKGATVKVKPSKL 728
              +LNYPS    F+    G+    + RT T+VG A + Y  K+ +   G  + V+P+ L
Sbjct: 666 SVADLNYPS----FAVNVDGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVL 725

Query: 729 AFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVT 770
            F  A +K+S+ V  + +    +  +    FGS+ WSDGKHVV +P+ ++
Sbjct: 726 NFKEANEKKSYTVTFTVDSSKPSGSN---SFGSIEWSDGKHVVGSPVAIS 755

[Close]
BLAST of CmoCh02G002480 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 721.1 bits (1860), Expect = 9.6e-208
Identity = 382/769 (49.67%), Postives = 510/769 (66.32%), Query Frame = 0 

Query: 1   MAACFDASLSSSIFFSLLLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTSEFA 60
           MA+   +S S +I  + L L+  +T       KT+I RV+   KP  F TH  WYTS+  
Sbjct: 1   MASSSSSSSSITIITTFLFLLLHTTAK-----KTYIIRVNHSDKPESFLTHHDWYTSQLN 60

Query: 61  ESHKILHVYDTVFHGFSATFTQEQADS-VGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQ 120
               +L+ Y T FHGFSA     +ADS +    S+L +FED    LHTTR+P+FLGL ++
Sbjct: 61  SESSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSE 120

Query: 121 RGLWSDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKI 180
            G+       + VIIGV DTG+ PE RSF D ++  IP +WKG C++G+ F +K CN+K+
Sbjct: 121 FGVHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKL 180

Query: 181 VGARFFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYA 240
           +GAR FSKG +  +       G +   E  SPRD DGHGTHT++TAAG     AS  GYA
Sbjct: 181 IGARSFSKGFQMASGG-----GFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYA 240

Query: 241 SGIAKGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYY 300
           +G A+G+A +AR+A YKVCW ++GCF SDILAA D A+ DGVDV+S+S+GGG   S PYY
Sbjct: 241 AGTARGMATRARVATYKVCW-STGCFGSDILAAMDRAILDGVDVLSLSLGGG---SAPYY 300

Query: 301 LDPIAIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNR 360
            D IAIGA+ A  RG+FVS SAGN GP   SV N+APW+ TVGAGT+DR+FPA   +GN 
Sbjct: 301 RDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNG 360

Query: 361 RKIHGVSLYAGAPLNSTMYPLVYPGKSGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 420
           +++ GVSLY+G  + +    LVY  K    S++LC+  SLD  IV GKIV+CDRG + RV
Sbjct: 361 KRLTGVSLYSGVGMGTKPLELVY-NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARV 420

Query: 421 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 480
           EKG VV+ AGG+GMI+AN  ++GE LV D+H+LPA AVG   GD ++ Y  S   PTA +
Sbjct: 421 EKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALL 480

Query: 481 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 540
            F+GT + +KP+PVVA+FS+RGPN + PEILKPD+I PGVNILA W+D +GPTGLD D+R
Sbjct: 481 VFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR 540

Query: 541 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 600
           +T+FNI+SGTSM+CPHISG A LLK+AHP+WSP+A++SA+MTTA + DN   P  + +  
Sbjct: 541 RTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADN 600

Query: 601 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGA-KMIQVISRTSVKCPAR 660
             S PY  G+GHV+   A+ PGLVYDI+  +Y+ FLCS+ Y    ++ ++ R SV C  +
Sbjct: 601 SLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKK 660

Query: 661 KPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPSK 720
              P  LNYPS    FS L  G     + R  TNVGAA+SVY+  +       + VKPSK
Sbjct: 661 FSDPGQLNYPS----FSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 720

Query: 721 LAF-STAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPL 767
           L+F S   KK+  V  +S    ++T     A FGS++WS+ +H VR+P+
Sbjct: 721 LSFKSVGEKKRYTVTFVSKKGVSMT---NKAEFGSITWSNPQHEVRSPV 747

[Close]
BLAST of CmoCh02G002480 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 689.1 bits (1777), Expect = 4.0e-198
Identity = 373/777 (48.01%), Postives = 498/777 (64.09%), Query Frame = 0 

Query: 18  LLLIHGSTVPGDPPLKTFIFRVDSFSKPSVFPTHFHWYTS------------EFAESHKI 77
           L+ +   T       KT++  +D  + P  +  H  WY+S            E   +++I
Sbjct: 20  LIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRI 79

Query: 78  LHVYDTVFHGFSATFTQEQADSVGKHPSVLAVFEDRRRQLHTTRSPQFLGLRNQRG--LW 137
           L+ Y T FHG +A  TQE+A+ + +   V+AV  + R +LHTTRSP FLGL  Q    +W
Sbjct: 80  LYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVW 139

Query: 138 SDSDYGSDVIIGVFDTGISPERRSFSDVNLGPIPRRWKGVCQTGTKFTAKNCNRKIVGAR 197
           ++     DV++GV DTGI PE  SF+D  + P+P  W+G C+TG +F  +NCNRKIVGAR
Sbjct: 140 AERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGAR 199

Query: 198 FFSKGHEAGANAAGPIIGINDTVEYRSPRDADGHGTHTASTAAGRHAFQASLEGYASGIA 257
            F +G+EA          I++ +EY+SPRD DGHGTHTA+T AG     A+L G+A G A
Sbjct: 200 VFYRGYEAATGK------IDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTA 259

Query: 258 KGVAPKARLAVYKVCWKNSGCFDSDILAAFDSAVKDGVDVISISIGGGDGVSTPYYLDPI 317
           +G+A KAR+A YKVCW   GCF SDIL+A D AV DGV V+SIS+GG  GVST Y  D +
Sbjct: 260 RGMAQKARVAAYKVCWV-GGCFSSDILSAVDQAVADGVQVLSISLGG--GVST-YSRDSL 319

Query: 318 AIGAYGAASRGIFVSSSAGNDGPNGMSVTNLAPWITTVGAGTIDRNFPAVVIMGNRRKIH 377
           +I  +GA   G+FVS SAGN GP+ +S+TN++PWITTVGA T+DR+FPA V +G  R   
Sbjct: 320 SIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFK 379

Query: 378 GVSLYAGAPL--NSTMYPLVYPGK--SGELSASLCMESSLDPKIVTGKIVICDRGSSPRV 437
           GVSLY G  +   +  YPLVY G+  S     S C++ +LD + V GKIVICDRG +PRV
Sbjct: 380 GVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRV 439

Query: 438 EKGLVVKKAGGVGMILANGISNGEGLVGDAHILPACAVGSVEGDAMKAYASSSGNPTATI 497
           +KG VVK+AGG+GM+L N  +NGE LV D+H+LPA AVG  EG  +K YA +S   TA++
Sbjct: 440 QKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASL 499

Query: 498 AFRGTRIGIKPAPVVASFSARGPNGLNPEILKPDMIAPGVNILAAWTDVVGPTGLDFDTR 557
              GTRIGIKP+PVVA+FS+RGPN L+ EILKPD++APGVNILAAWT  + P+ L  D R
Sbjct: 500 EILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPR 559

Query: 558 KTEFNILSGTSMACPHISGAAALLKSAHPDWSPAAVRSAMMTTARITDNRRQPTTEESTG 617
           + +FNILSGTSM+CPH+SG AAL+KS HPDWSPAA++SA+MTTA + DN  +P T+ S  
Sbjct: 560 RVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGA 619

Query: 618 KASTPYDFGAGHVNLGLAMDPGLVYDITNTDYVNFLCSIGYGAKMIQVISRTSVKC--PA 677
             S+PYD GAGH++   A DPGLVYDI   +Y  FLC+       ++V ++ S +     
Sbjct: 620 APSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHT 679

Query: 678 RKPLPENLNYPSIVAVFSSLSKGLSTKSFIRTATNVGAANSVYQAKIEAPKGATVKVKPS 737
               P NLNYP+I A+F   +  +   +  RT TNVG   S Y+  +   KGA+V V+P 
Sbjct: 680 LAKNPGNLNYPAISALFPE-NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPK 739

Query: 738 KLAFSTAVKKQSFVVAISANYPNLTLGDVGAVFGSLSWSDGKHVVRTPLVVTKLEPL 775
            L F++  +K S+ V     +           FG L W    H VR+P+++T L PL
Sbjct: 740 TLNFTSKHQKLSYTVTFRTRFRMKR-----PEFGGLVWKSTTHKVRSPVIITWLPPL 780

The following BLAST results are available for this feature:
BLAST of CmoCh02G002480 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O496070.0e+0072.23Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Q9LUM33.1e-24356.87Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O653515.5e-20850.65Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF61.4e-20649.67Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9FLI45.7e-19748.01Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
BLAST of CmoCh02G002480 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G5740.0e+00100.00subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111450975 PE... [more]
A0A6J1I5D00.0e+0096.64subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111469756 PE=3... [more]
A0A6J1JEF50.0e+0089.47subtilisin-like protease SBT1.6 OS=Cucurbita maxima OX=3661 GN=LOC111483679 PE=3... [more]
A0A6J1FYE50.0e+0089.00subtilisin-like protease SBT1.6 OS=Cucurbita moschata OX=3662 GN=LOC111448551 PE... [more]
A0A0A0LJ580.0e+0089.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G270180 PE=3 SV=1[more]
BLAST of CmoCh02G002480 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022946948.10.0e+00100.00subtilisin-like protease SBT1.6 [Cucurbita moschata][more]
KAG6604929.10.0e+0099.22Subtilisin-like protease 1.6, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_023532451.10.0e+0097.93subtilisin-like protease SBT1.6 [Cucurbita pepo subsp. pepo][more]
XP_022970938.10.0e+0096.64subtilisin-like protease SBT1.6 [Cucurbita maxima][more]
XP_038901136.10.0e+0090.44subtilisin-like protease SBT1.6 [Benincasa hispida][more]
BLAST of CmoCh02G002480 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G34980.10.0e+0072.23subtilisin-like serine protease 2 [more]
AT3G14240.12.2e-24456.87Subtilase family protein [more]
AT5G67360.13.9e-20950.65Subtilase family protein [more]
AT2G05920.19.6e-20849.67Subtilase family protein [more]
AT5G51750.14.0e-19848.01subtilase 1.3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 213..226
score: 50.0
coord: 547..563
score: 58.55
coord: 129..148
score: 32.4
NoneNo IPR availableGENE3D2.60.40.2310coord: 635..771
e-value: 7.3E-38
score: 131.2
NoneNo IPR availableGENE3D3.50.30.30coord: 347..487
e-value: 1.8E-177
score: 592.8
NoneNo IPR availablePANTHERPTHR10795:SF675SUBTILISIN-LIKE PROTEASE SBT1.7coord: 22..768
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 104..618
score: 29.905037
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..479
e-value: 6.19914E-38
score: 135.619
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 404..473
IPR003137PA domainPFAMPF02225PAcoord: 378..464
e-value: 3.6E-9
score: 36.6
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 48..106
e-value: 1.8E-10
score: 41.4
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 129..593
e-value: 5.9E-47
score: 161.2
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 131..631
e-value: 1.8E-177
score: 592.8
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 105..622
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 664..768
e-value: 9.8E-27
score: 93.1
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 30..106
e-value: 4.6E-12
score: 48.2
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 22..768
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 548..558
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 104..583
e-value: 6.60719E-144
score: 422.777

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G002480.1CmoCh02G002480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity