CmoCh02G002380 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G002380
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionnitrile-specifier protein 5
LocationCmo_Chr02: 1119665 .. 1120918 (-)
RNA-Seq ExpressionCmoCh02G002380
SyntenyCmoCh02G002380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCGCCAAAGACTGCCCTAGTCACACTCGACGCCATTGCTATCTGCCGATTGAGCTCTGGACTTTCCGCAAATGGCTCCGATGAACGGAAAATGGACGAAGGTCAGTCCCTTAAGCTAATTAAGCTCATTGATTCAATCTTCAATTCACCATTTTATGAATTCATCTTCATAATTTCAACTTCTATTGCCACAAATTTCATTTACTTTTCGAAATCTTGATCAATTTGTTGCAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAACAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTGGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGAGCGAGAATGGTAAATGTAGTTGACGGTCCTGATTTGATCGATTCCTATGATGGGTGTGATTATGTGTGTATTTCGAGT

mRNA sequence

CGCGCCAAAGACTGCCCTAGTCACACTCGACGCCATTGCTATCTGCCGATTGAGCTCTGGACTTTCCGCAAATGGCTCCGATGAACGGAAAATGGACGAAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAACAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTGGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGAGCGAGAATGGTAAATGTAGTTGACGGTCCTGATTTGATCGATTCCTATGATGGGTGTGATTATGTGTGTATTTCGAGT

Coding sequence (CDS)

ATGGCTCCGATGAACGGAAAATGGACGAAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAACAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTGGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGA

Protein sequence

MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVYGGNSPSNDRLGDIYFFTPV
Homology
BLAST of CmoCh02G002380 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 9.6e-106
Identity = 174/320 (54.37%), Postives = 224/320 (70.00%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           M P+  KW K+ Q GAGPGARSSHA+TVVG K Y FGGE  P I +DN+L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD + +  N L+S+DT  N+W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD ++ KW+EYP+AGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           V +G+IWV++GF G E+ D+H FD A  +W  VETTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E  P  RGW AF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of CmoCh02G002380 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 154/318 (48.43%), Postives = 218/318 (68.55%), Query Frame = 0

Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           ++GKWTK+D+NG GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD++  TW+++
Sbjct: 298 LHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIESRTWSIS 357

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPP 123
             +GD+P    +GV M ++G  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP 
Sbjct: 358 PATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPT 417

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V 
Sbjct: 418 PRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVV 477

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ A+GK++ I+GGE+
Sbjct: 478 QGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIVIFGGEI 537

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 303
               + H+G G+     + LDTE L+W R++   G E  P  RGW+A +    GG+ GL+
Sbjct: 538 AMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIGGKKGLV 597

Query: 304 VYGGNSPSNDRLGDIYFF 321
           ++GG +P+NDR  D++F+
Sbjct: 598 MHGGKAPTNDRFDDLFFY 614

BLAST of CmoCh02G002380 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 348.6 bits (893), Expect = 7.6e-95
Identity = 155/316 (49.05%), Postives = 213/316 (67.41%), Query Frame = 0

Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of CmoCh02G002380 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-91
Identity = 155/319 (48.59%), Postives = 214/319 (67.08%), Query Frame = 0

Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           + GKW K++Q G GPG R SH I  VG K ++FGGE +P  P+D  L+VFDL+  TW+++
Sbjct: 150 LRGKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSIS 209

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPP 123
             +GDVP    +GV M ++G  +YVFGGRD   K  N  YSFDT+ N+W LL+  E GP 
Sbjct: 210 PATGDVPNLSCLGVRMVSIGSSLYVFGGRDASRK-YNGFYSFDTTKNEWKLLTPVEQGPT 269

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS TAD+  VY+FGG   + RL  L AY+ ++ KW++  + G     RGG GL V 
Sbjct: 270 PRSFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVV 329

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVH +DPAQ+KW QVET GE+P ARSVF++  +GK++ ++GGE+
Sbjct: 330 QGKVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEI 389

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENH-PGPRGWSAFSVGRKGGELGL 303
                 H G G+ +G  + LDTE L+W ++ D LG E   P  RGWSA + G   G+ GL
Sbjct: 390 AMDPKAHEGPGQLSGGTFALDTETLKWEKL-DKLGEEEETPSIRGWSASTTGTIDGKKGL 449

Query: 304 LVYGGNSPSNDRLGDIYFF 321
           +++GG + +NDR GD++F+
Sbjct: 450 VMFGGKAQTNDRFGDLFFY 466

BLAST of CmoCh02G002380 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.3e-90
Identity = 148/316 (46.84%), Postives = 206/316 (65.19%), Query Frame = 0

Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           G W K++QNG GPG R SH I  VG K Y+FGGE  P  P+D  L+VFDL+  TW++A  
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +Y FGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG G   R+  L +Y+ ++  W    + G+    RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+W+VYGF G E+DDVH++DPA++KW QVET G +P  RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
               H+G G+     + LDTE L+W R++     E  P  RGW+A + G   G+ GL+++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

BLAST of CmoCh02G002380 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.3e-195
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 2.6e-191
Identity = 315/323 (97.52%), Postives = 319/323 (98.76%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDN+LHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTM AVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTI+GKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPT RSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.9e-175
Identity = 282/322 (87.58%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

BLAST of CmoCh02G002380 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.9e-175
Identity = 282/322 (87.58%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

BLAST of CmoCh02G002380 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 278/322 (86.34%), Postives = 297/322 (92.24%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP  G W KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LH+FDL +H W
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TV + SGD+PPPRVGVTMA+VGQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGG+ G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDTI+GKWIEYP+AGEGLKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYF P QNKW QVET GERPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKF GEVYVLDTEALEWSRVEDGLGLE+HPGPRGW AF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDI+FF P
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAP 322

BLAST of CmoCh02G002380 vs. NCBI nr
Match: XP_022947523.1 (nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 691.0 bits (1782), Expect = 4.8e-195
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. NCBI nr
Match: KAG7026998.1 (Nitrile-specifier protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 688.7 bits (1776), Expect = 2.4e-194
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. NCBI nr
Match: XP_023533621.1 (nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.2 bits (1759), Expect = 2.2e-192
Identity = 318/323 (98.45%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYS DTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSLDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKG ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGRELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 677.6 bits (1747), Expect = 5.5e-191
Identity = 315/323 (97.52%), Postives = 319/323 (98.76%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDN+LHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTM AVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTI+GKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPT RSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

BLAST of CmoCh02G002380 vs. NCBI nr
Match: XP_008464157.1 (PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >KAA0060815.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa] >TYK08820.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 282/322 (87.58%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

BLAST of CmoCh02G002380 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-137
Identity = 214/320 (66.88%), Postives = 254/320 (79.38%), Query Frame = 0

Query: 3   PMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTV 62
           PM GKW ++ Q G GPGARSSHAI +VG K YAFGGEF PR+PVDN+L+VFDL+  TW++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  ASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPP 122
              SGD PPPRVGV MAAVG I+Y FGGRD  H+ELNELY F+T  N+W LLS GE+GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 182
           +RSYHS TAD + VY+FGGCG  GRLNDLWAY+ ++ KWI++PS GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 242
           QGKIWVVYGFAG E DDVH FD A+ +W +VET GE+P+ARSVFST  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 302
           DPSD+GH+GAG F G+ Y LDTE LEW + EDG+G   HPGPRGW AF+ G + G+ GLL
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFTP 323
           VYGGNSPSNDRL DI+FFTP
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

BLAST of CmoCh02G002380 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 384.8 bits (987), Expect = 6.8e-107
Identity = 174/320 (54.37%), Postives = 224/320 (70.00%), Query Frame = 0

Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           M P+  KW K+ Q GAGPGARSSHA+TVVG K Y FGGE  P I +DN+L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD + +  N L+S+DT  N+W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD ++ KW+EYP+AGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           V +G+IWV++GF G E+ D+H FD A  +W  VETTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E  P  RGW AF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of CmoCh02G002380 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 354.8 bits (909), Expect = 7.6e-98
Identity = 154/318 (48.43%), Postives = 218/318 (68.55%), Query Frame = 0

Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           ++GKWTK+D+NG GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD++  TW+++
Sbjct: 298 LHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIESRTWSIS 357

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPP 123
             +GD+P    +GV M ++G  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP 
Sbjct: 358 PATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPT 417

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V 
Sbjct: 418 PRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVV 477

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ A+GK++ I+GGE+
Sbjct: 478 QGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIVIFGGEI 537

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 303
               + H+G G+     + LDTE L+W R++   G E  P  RGW+A +    GG+ GL+
Sbjct: 538 AMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIGGKKGLV 597

Query: 304 VYGGNSPSNDRLGDIYFF 321
           ++GG +P+NDR  D++F+
Sbjct: 598 MHGGKAPTNDRFDDLFFY 614

BLAST of CmoCh02G002380 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 348.6 bits (893), Expect = 5.4e-96
Identity = 155/316 (49.05%), Postives = 213/316 (67.41%), Query Frame = 0

Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of CmoCh02G002380 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 348.6 bits (893), Expect = 5.4e-96
Identity = 155/316 (49.05%), Postives = 213/316 (67.41%), Query Frame = 0

Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93XW59.6e-10654.38Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1[more]
O043161.1e-9648.43Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1[more]
Q9SDM97.6e-9549.05Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2[more]
O493261.3e-9148.59Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1[more]
O043184.3e-9046.84Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G6U42.3e-195100.00nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 S... [more]
A0A6J1I5Q12.6e-19197.52nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=... [more]
A0A1S3CLA85.9e-17587.58nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1[more]
A0A5D3CBW15.9e-17587.58Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0KDY91.2e-17286.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022947523.14.8e-195100.00nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier... [more]
KAG7026998.12.4e-19499.69Nitrile-specifier protein 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023533621.12.2e-19298.45nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo][more]
XP_022971053.15.5e-19197.52nitrile-specifier protein 5 [Cucurbita maxima][more]
XP_008464157.11.2e-17487.58PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED:... [more]
Match NameE-valueIdentityDescription
AT3G07720.11.3e-13766.88Galactose oxidase/kelch repeat superfamily protein [more]
AT5G48180.16.8e-10754.38nitrile specifier protein 5 [more]
AT3G16410.17.6e-9848.43nitrile specifier protein 4 [more]
AT3G16400.15.4e-9649.05nitrile specifier protein 1 [more]
AT3G16400.25.4e-9649.05nitrile specifier protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 185..233
e-value: 6.2
score: 4.7
coord: 135..184
e-value: 0.08
score: 21.3
coord: 84..134
e-value: 17.0
score: 0.9
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 174..213
e-value: 2.8E-6
score: 26.8
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 1..196
e-value: 2.3E-44
score: 153.6
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 197..323
e-value: 1.7E-16
score: 62.0
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 3..243
NoneNo IPR availablePFAMPF13418Kelch_4coord: 222..274
e-value: 1.8E-5
score: 24.6
coord: 123..161
e-value: 6.4E-11
score: 42.1
NoneNo IPR availablePFAMPF13415Kelch_3coord: 30..80
e-value: 2.7E-10
score: 40.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR47435:SF4KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)coord: 4..322
NoneNo IPR availablePANTHERPTHR47435KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)coord: 4..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G002380.1CmoCh02G002380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0080028 nitrile biosynthetic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0010043 response to zinc ion
cellular_component GO:0005634 nucleus
molecular_function GO:0030234 enzyme regulator activity
molecular_function GO:0005515 protein binding