CmoCh02G001430 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G001430
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Auxin-responsive GH3 family protein
Location	Cmo_Chr02: 711031 .. 713842 (+)
RNA-Seq Expression	CmoCh02G001430
Synteny	CmoCh02G001430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCGTATCGGAATTCTTAACCAGGTGAGTGTAAACAAATCTATTGTAGTTATTCTTGTTAGAGACTACGACTCTTCACAATCGTTTGATATTGTCTACTTTCAGTATAAGCTCTCATAATTTTGAATAAAGTATACCATAGAGCCTCCCCTAAGGCTTACGGAGCCCTCGAACAACCTCCCCTTAATCGAGGCTCAACTCCTTGTCTGGAGCTTTTGAACAAAGTAAACCCTTCGTTCAATACTTTAGTCACTTTTGACTACACTTTCGAGGCTCACAATTTCTTTGTTCGACATTTGAGAATTCTATTGATATGGCTAAGTTAAGGGCATGGCTCTAATACCATGATAGGAACCATGACTCTCCACAATGGTATGATATTGTCCATTTTGAGCATAAGCTCTCGTGGCTTTGCTTTTGGTTTCTCCAAAAGACATCATACTTAGCCGATGATCAACCCCTCAATTAGCCGTTGTGGTACTCCTCTCCCAATTGTGTTTGAACTCAATTGTTGTTTGTGTTTTTCTATTCTAGTTCCGGAACATCGGGAGGGGAGAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGGGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTATCATACCTAGGCTGTGGCCAAATACAAAGTATATTGATGTTATTGTGACAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGGTATGTTTTTGTTGAGACATAGAGAATCCATGGGTCTGCCGCCTATTTATAGCTTATTCAATTGTTATAACTGTTAGACACAATTGTTTAGACTCTATGGGGAGCCTGTAGACACAATTGTTTAGACCCTATGGGGAGCCTGTAGACACAATTGTTTAGACCCTATGGGGAGCATGTAGACACAATTGTTTAGACTCTACAGAGCCCTGTAGACTCAATTGTTTCCTGTCTATGACAGTGTGTATAGTTTCCTGTCTATGACAGTGTGTATAGTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGAGTGTATTGATTAAGCTATAAATAGACAGCTGTCTATGAGTGTATAGTTTTCCTGTCTGGTTGATTAAGCTATAAATAGACAGCAGACTCCTACATGAATGAATAATATTTGGTTCAGGTATCTAAGATAACCTATCTTATTTGATATTGCAGGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATGGGTCCCTGGTCAAAAAGAATGGTCCACGAAGAAGCGTAGTTAA

mRNA sequence

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGGGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGAGAATCCATGGGTCTGCCGCCTATTTATAGCTTATTCAATTGTTATAACTGTTAGACACAATTGTTTAGACTCTATGGGGAGCCTTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATGGGTCCCTGGTCAAAAAGAATGGTCCACGAAGAAGCGTAGTTAA

Coding sequence (CDS)

ATGCCGGGGAAAGATGAAGGTGCACTATTGCAGCATATTGAAGAGATTACAACTCATGCTGATCAAGTTCAACGAAGGGTTCTTACTGAAATTCTCTCCCGCAACGCCCATGTTGAGTACCTACAACAACAAGGGCTTGATGGGTGTATGGATAGGATGGCATTCAAGAACATAGTTCCCGTTGTTTCCTATGAGAATTTAAAGCCATATATCGATCGAATTGCTAACGGCGATGCTTCTCCAATTCTATGTTCCGAACCCAGGAAATTAATGCCGACAACCGAAGAAGAGCTGAAGAGAAGATCATTTCTTTACTCCCTTTTGATGCCTGTGATGAACCAATTCATTCCAGGCTTAAACAAGGGGAAAGGAATGTACTTTTTGTTCGTAAAATCCGAATCCACAACTCCTGGAGGATTACCAGCTCGTCCAGTTCTCACTAGCTACTACAAAAGCTCACACTTTAAGGACCGCCCATATGATCTCTACACTGATTACACCAGCCCTAACCAAACAATTCTTTGCACAGACTCATATCAAAGCATGTACTGTCAGTTCCTTTGTGGGTTATGTCTGAGCCAGCAAGTCCTTAGAGTTGGCTCAGTTTTCGCATCTGGGTTCATTCGAGCCATCAAATTCCTTGAAACCCATTGGGTCCTTTTGGCTAATGATATCAGAACAGGAACCATAAACCCTAGAATCAGGGACCCATTAGTGAGACAATCAGTCATGAACATTCTGAAACCTGACCCTGTGCTGGCTGATTTGATTGAATTTGAGTGCAGCAAAGGGACGTGGGCAGGTACTATGTCACAGTATATCCCAACTTTGGATTACTATAGCAATGGCCTCCCTCTTGTTTGCACCATGTATGCTTCTTCTGAGTGCTACTTTGGGGTTAATCTAAACCCTTTGTGTAACCCAAATGAGGTCTCTTACACGCTTATTCCCACCATGGCATATTTTGAATTCTTACCTCTTGATAGGAAGATGAGTGAAGAATTGGTTGATCTTGTGGACGTTGAACTGGGAAAAGAATATGAGCTCGTTATCACTACTTATTCAGAGAATCCATGGGTCTGCCGCCTATTTATAGCTTATTCAATTGTTATAACTGTTAGACACAATTGTTTAGACTCTATGGGGAGCCTTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGTGTGTATAGTTTTCCTGTCTATGACAGGCTATATCGATATCGAGTTGGTGACATTCTTCGAGTATCAGGATTCAAGAACAAGGCTCCTCAATTCAACTTCGTATGTCGGAAAAATGTGATTCTCAGCATCGACTCCGACAAAGTAGACGAAGTTGAGCTTCAAAACGCAGTCATGGAAGCTTCTAAGAACTTAATCCCATATGAAACTAGCCTCCTTGAGTACACGAGCTACGCCGATACTCATACAGTTCCAGGGCATTATGTCATATATTGGGAAATTAGCCAAAAGGAAACCAACGCCATTCCCGATTTGGTATTTGAAAATTGTTGCCTAACCATCGAGGAATCACTCAACAGCGTGTATCGTCAAGGACGAGTCTCGGATAAATCAATTGGTCCTTTGGAGATAAAGATTATCGAGAATGGGACGTTTGACAAACTCATGGATTATGCCATCAATCATGGATCTTCCATAAACCAATACAAAACCCCTCGTTGCGTGAAACACGAACCAATTCTCGAGCTTTTGAACTCGTACGTTGTGTCCAATTATTTCAGCCCGAGATGTCCCAAATGGGTCCCTGGTCAAAAAGAATGGTCCACGAAGAAGCGTAGTTAA

Protein sequence

MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKPYIDRIANGDASPILCSEPRKLMPTTEEELKRRSFLYSLLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS

Homology

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BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.4e-229
Identity = 404/642 (62.93%), Postives = 466/642 (72.59%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P              RKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I 
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W                   GTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                   K  +ELVDLVDV+LG+EYELV+TTY+                          
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA-------------------------- 444

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         LYRYRVGD+L V+GFKN APQF+F+CRKNV
Sbjct: 445 -----------------------------GLYRYRVGDVLSVAGFKNNAPQFSFICRKNV 504

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+      
Sbjct: 505 VLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLNGNT 564

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 604
            IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ G+SIN
Sbjct: 565 PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISLGASIN 611

[Close]

BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 1.7e-228
Identity = 401/642 (62.46%), Postives = 466/642 (72.59%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQR+VL EIL+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P              RKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I 
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W                   GTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                   K  +ELVDLVDV+LG+EYELV+TTY+                          
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA-------------------------- 444

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         L RYRVGD+LRV+GFKNKAPQF+F+CRKNV
Sbjct: 445 -----------------------------GLCRYRVGDLLRVTGFKNKAPQFSFICRKNV 504

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+      
Sbjct: 505 VLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLDGNT 564

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 604
            IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ G+SIN
Sbjct: 565 PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASIN 611

[Close]

BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 6.0e-194
Identity = 347/635 (54.65%), Postives = 427/635 (67.24%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRM-AFKNIVPVVSYENLK 69
           L+ IE +T +A QVQRRVL EIL++NA  EYL++ G+ G  D + AF+ +VP+V+YE L+
Sbjct: 24  LEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEGLQ 83

Query: 70  PYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQ 129
           P I RIANGD SPI   +P              RKLMPT  +E+ RRS LYSLLMPVM+Q
Sbjct: 84  PDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQ 143

Query: 130 FIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTIL 189
            + GL+KGK MY LFVK+ES TPGGL ARPVLTSYY+S  F DRP D YT YTSP++ IL
Sbjct: 144 SVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAIL 203

Query: 190 CTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRI 249
           C DSYQSMY Q LCGL     VLRVG+VFASGF+RAI FLE HW  L +DIRTG ++P I
Sbjct: 204 CVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEI 263

Query: 250 RDPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPT 309
            D +VR +V  +L+ DP LAD IE EC++ +W                   GTMSQYIPT
Sbjct: 264 TDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD------RK 369
           L++Y  GLPL CTMYASSECYFG+NLNP+C P++V+YTLIPTM Y+EFLP++        
Sbjct: 324 LEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAEA 383

Query: 370 MSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSFPV 429
              +LVDLVDV+LG EYELV+TTYS                                   
Sbjct: 384 SHRDLVDLVDVKLGHEYELVVTTYS----------------------------------- 443

Query: 430 YDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSDKV 489
                                LYRYRVGD+LRV+GFKNKAP F+FV R+NV LS+DSDK 
Sbjct: 444 --------------------GLYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKT 503

Query: 490 DEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVFEN 549
           DE EL  AV  A ++L P+  SL+EYTSYAD  T+PGHYV++WE+    T A+P  VFE 
Sbjct: 504 DETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELRAGST-AVPASVFEE 563

Query: 550 CCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVK 606
           CCL++EE+LNSVYRQGR  D+SIGPLEI+++  GTFDKLMDYAI+ G+SINQYK PRCV+
Sbjct: 564 CCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYKAPRCVR 602

[Close]

BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 658.3 bits (1697), Expect = 8.5e-188
Identity = 342/629 (54.37%), Postives = 418/629 (66.45%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + P              RKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR+ 
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------GTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WA                  G M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSFPVYD 429
            +LVDL  VE+G+EYELVITTY+                                     
Sbjct: 390 TQLVDLARVEVGREYELVITTYA------------------------------------- 449

Query: 430 SVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSDKVDE 489
                              L RYRVGD+LRV+GF N APQF FV RKNV+LSI+SDK DE
Sbjct: 450 ------------------GLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDE 509

Query: 490 VELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPDLVFEN 549
            ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +       
Sbjct: 510 AELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADTLGR 569

Query: 550 CCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVK 600
           CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK PRCV 
Sbjct: 570 CCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVT 603

[Close]

BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 8.5e-188
Identity = 342/629 (54.37%), Postives = 418/629 (66.45%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ I+E+TT+ D VQ RVL EIL RNA  EYL + GLDG  DR AF+  VPVVSY++L+P
Sbjct: 30  LRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVSYDDLQP 89

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           YI RIANGD SPIL + P              RKLMPT  +EL RR  LYSLLMPVMN +
Sbjct: 90  YIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLY 149

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSE+ TPGGL ARPVLTSYYKS HFK+RPYD Y +YTSP   ILC
Sbjct: 150 VPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILC 209

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
            D++QSMY Q +CGLC    VLR+G+VFASG +RAI+FL+ +W  LA+DI +G + PR+ 
Sbjct: 210 ADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVT 269

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------GTMSQYIPTL 309
           DP VR++V  IL PDP LA LI  ECSKG WA                  G M+QYIPTL
Sbjct: 270 DPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTL 329

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRK-----MS 369
           ++YS GLP+ CTMYASSECYFG+NL P+C+P+EVSYT++P M YFEFLP+D        +
Sbjct: 330 EFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDA 389

Query: 370 EELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSFPVYD 429
            +LVDL  VE+G+EYELVITTY+                                     
Sbjct: 390 TQLVDLARVEVGREYELVITTYA------------------------------------- 449

Query: 430 SVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSDKVDE 489
                              L RYRVGD+LRV+GF N APQF FV RKNV+LSI+SDK DE
Sbjct: 450 ------------------GLNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDE 509

Query: 490 VELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNA--IPDLVFEN 549
            ELQ AV  AS  L P+  S++EYTS A T  +PGHYVIYWE+  K   A  +       
Sbjct: 510 AELQRAVERASALLRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADTLGR 569

Query: 550 CCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVK 600
           CCL +EE+LN+VYRQ RV+D SIGPLEI+++  GTF++LMDYAI+ G+SINQYK PRCV 
Sbjct: 570 CCLEMEEALNTVYRQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVT 603

[Close]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL
Match: A0A6J1G8Z2 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111451824 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 551/639 (86.23%), Postives = 551/639 (86.23%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTTEEELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYELVITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 584

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS
Sbjct: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 584

[Close]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL
Match: A0A6J1I4E6 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111469778 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 1.1e-307
Identity = 541/639 (84.66%), Postives = 545/639 (85.29%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTT+EELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLD YSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYEL+ITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELIITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD VF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDWVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQYKTPRC 584

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VK EPILELLNSYVVSNYFSP+CPKWVPGQKEWSTKK S
Sbjct: 601 VKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTKKHS 584

[Close]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 9.5e-259
Identity = 461/647 (71.25%), Postives = 499/647 (77.13%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ+IE++T HADQVQRRVLTEILS NAHVEYL + GLDG MD   FK  +PVVSY+++KP
Sbjct: 27  LQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVSYDDIKP 86

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
           Y+DRIANGD SPILCS+P              RKLMPTTEEEL+R+S LYSLLMPVMNQF
Sbjct: 87  YVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLMPVMNQF 146

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           IPGL+KGKGMYFLFVKSESTTPGGL ARPVLTSYY+S HFKDRPYD Y  YTSPN+TILC
Sbjct: 147 IPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSPNETILC 206

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           TDSYQSMY QFLCGLCLS++VLRVG+VFASGFIRAIKFLETHW+LL+NDIRTGTINPRI 
Sbjct: 207 TDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGTINPRIT 266

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DPLVRQSV+NILKPDP LADLIE ECSKGTW                   GTMSQYIPTL
Sbjct: 267 DPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMSQYIPTL 326

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD--------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLCNP+EVSYTLIPTMAYFEFLPLD         
Sbjct: 327 DYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTNGFGYSS 386

Query: 370 -------RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                  +K  +ELVDLVDV+LGKEYELVITTY+                          
Sbjct: 387 TCQSEANQKTDQELVDLVDVQLGKEYELVITTYA-------------------------- 446

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         LYRYRVGDILRVSGFKN APQFNFVCRKNV
Sbjct: 447 -----------------------------GLYRYRVGDILRVSGFKNNAPQFNFVCRKNV 506

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKE-- 549
           ILSIDSDKVDEVELQNAVMEASKNLIPY   ++EYTSYADT T+PGHYVIYWEIS KE  
Sbjct: 507 ILSIDSDKVDEVELQNAVMEASKNLIPYGACVVEYTSYADTVTIPGHYVIYWEISLKEAT 566

Query: 550 ---TNAIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINH 604
               N IP  VFE CCL++EESLNSVYRQGRVSDKSIGPLE+KI+E GTFDKLMDYA+NH
Sbjct: 567 ANNNNVIPGSVFEKCCLSMEESLNSVYRQGRVSDKSIGPLEMKIVETGTFDKLMDYALNH 617

[Close]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL
Match: A0A1U7YQ53 (indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC104585725 PE=3 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.3e-235
Identity = 414/646 (64.09%), Postives = 478/646 (73.99%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IE++TT+AD++Q+RVL EILSR+AHVEYLQ+ GL+G  DR  FK ++PVV+YE+L+ 
Sbjct: 31  LEFIEDVTTNADEIQKRVLAEILSRSAHVEYLQRHGLNGRTDRETFKKVMPVVTYEDLQS 90

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPILC  P              RKLMPT EEEL+RRS LYSLLMPVM+QF
Sbjct: 91  DINRIANGDTSPILCGLPISEFLTSSGTSAGERKLMPTIEEELERRSLLYSLLMPVMDQF 150

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKS HFK RPYD YT+YTSPN+TILC
Sbjct: 151 VPGLDKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSLHFKHRPYDPYTNYTSPNETILC 210

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
            DSYQSMY Q LCGLC ++QVLRVG+VFASGFIRAI+FLE HW LL NDIRTG INP+I 
Sbjct: 211 PDSYQSMYSQLLCGLCQNKQVLRVGAVFASGFIRAIRFLEKHWSLLCNDIRTGAINPQIT 270

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DP VR +V+ ILKPDP LAD IE EC++  W                   GTMSQYIPTL
Sbjct: 271 DPSVRDAVLRILKPDPELADFIESECTREAWQGIITRLWPNTKYIDVIVTGTMSQYIPTL 330

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP----------- 369
           DYYSNGLPLVCTMYASSECYFG+NLNPLC PNEVSYTLIPTMAYFEFLP           
Sbjct: 331 DYYSNGLPLVCTMYASSECYFGLNLNPLCKPNEVSYTLIPTMAYFEFLPVHRNNGFTTSI 390

Query: 370 -----LDRKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                L+ K  ++LVDLVDV+LG+EYELV+TTY+                          
Sbjct: 391 SVPKSLNEKEQQDLVDLVDVKLGQEYELVVTTYA-------------------------- 450

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         LYRYRVGD+LRV+GFKNKAPQFNFVCRKNV
Sbjct: 451 -----------------------------GLYRYRVGDVLRVAGFKNKAPQFNFVCRKNV 510

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQ AV +A  +L+P++ SL+EYTSYADT T+PGHYV+YWE+    T 
Sbjct: 511 VLSIDSDKTDEVELQAAVKDAINHLVPFDASLIEYTSYADTTTIPGHYVLYWELRHDSTT 570

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 608
            IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ G+SIN
Sbjct: 571 PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKIVETGTFDKLMDYAISRGASIN 621

[Close]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL
Match: A0A7J7C2B3 (Putative Indole-3-acetic acid-amido synthetase GH3.6 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW21G00139 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 1.1e-233
Identity = 410/644 (63.66%), Postives = 480/644 (74.53%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++T++AD+VQ++VL EILS NAHVEYLQ+ GL G  DR  FK IVP ++YE+++ 
Sbjct: 24  LQFIEDVTSNADEVQKKVLEEILSHNAHVEYLQRHGLKGHTDRETFKKIVPAITYEDIQS 83

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPILCS+P              RKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 84  DINRIANGDTSPILCSKPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLMPVMSQF 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL KGKGMYFLF+KSE+ TPGGL ARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 144 VPGLEKGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFKNRPYDPYTNYTSPNETILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
            DSYQSMY Q LCGLC +++VLRVG+VFASGFIRAI+FLE HW LL +DIRTGTINP+I 
Sbjct: 204 PDSYQSMYSQMLCGLCQNKEVLRVGAVFASGFIRAIRFLEKHWPLLCSDIRTGTINPQIT 263

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DP VR++VM ILKPDP LAD ++ EC K +W                   GTMSQYIPTL
Sbjct: 264 DPSVREAVMKILKPDPKLADFVQAECGKDSWKGIITKLWPNTKYIDVIVTGTMSQYIPTL 323

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNLNPLC+P+EVSYTLIPTMAYFEFLP++R        
Sbjct: 324 DYYSNGLPLVCTMYASSECYFGVNLNPLCDPSEVSYTLIPTMAYFEFLPVNRSNGATKLI 383

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                   K  ++LVDLVDV+LG+EYELV++TY+                          
Sbjct: 384 SMPKTLNEKEQQKLVDLVDVKLGQEYELVVSTYA-------------------------- 443

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         LYRYRVGD+LRV+GFKNKAPQFNF+CRKNV
Sbjct: 444 -----------------------------GLYRYRVGDVLRVAGFKNKAPQFNFICRKNV 503

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQNAV  A  NL+P++ +L EYTSYADT T+PGHYV++WE++   + 
Sbjct: 504 VLSIDSDKTDEVELQNAVKNAMNNLVPFDATLAEYTSYADTTTIPGHYVLFWELTLNGST 563

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 606
           +IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPL+IKI+E GTFDKLMDYAI+ G+SIN
Sbjct: 564 SIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLQIKIVEPGTFDKLMDYAISLGASIN 612

[Close]

BLAST of CmoCh02G001430 vs. NCBI nr
Match: XP_022948165.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 551/639 (86.23%), Postives = 551/639 (86.23%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTTEEELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYELVITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 584

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS
Sbjct: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 584

[Close]

BLAST of CmoCh02G001430 vs. NCBI nr
Match: KAG6604833.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 548/639 (85.76%), Postives = 550/639 (86.07%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTT+EELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYELVITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 584

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VKHEPILELLNSYVVSNYFSPRCPKWVPG+KEWSTKKRS
Sbjct: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGKKEWSTKKRS 584

[Close]

BLAST of CmoCh02G001430 vs. NCBI nr
Match: XP_023533206.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 541/639 (84.66%), Postives = 548/639 (85.76%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MP +DEGA+LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 8   MPERDEGAILQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 67

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTTEEELKRRSFLYS
Sbjct: 68  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 127

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLN+GKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 128 LLMPVMNQFIPGLNRGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 187

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 188 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 247

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 248 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 307

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 308 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 367

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           R+MSEELVDLVDVELGKEYELVITTYS                                 
Sbjct: 368 REMSEELVDLVDVELGKEYELVITTYS--------------------------------- 427

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 428 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 487

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNL+PYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF
Sbjct: 488 KVDEVELQNAVMEASKNLVPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 547

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 548 ENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQYKTPRC 591

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWS+KKRS
Sbjct: 608 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSSKKRS 591

[Close]

BLAST of CmoCh02G001430 vs. NCBI nr
Match: XP_022970970.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.3e-307
Identity = 541/639 (84.66%), Postives = 545/639 (85.29%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTT+EELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPISEFLTSSGTSGGERKLMPTTKEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMY QFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYSQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIITRLWPNTKYVDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLD YSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDKYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYEL+ITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELIITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD VF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDWVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 600
           ENCCLTIEESLNSVYRQGRVSDKSIGPLE+KIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLELKIIENGTFDKLMDYAINHGSSINQYKTPRC 584

Query: 601 VKHEPILELLNSYVVSNYFSPRCPKWVPGQKEWSTKKRS 608
           VK EPILELLNSYVVSNYFSP+CPKWVPGQKEWSTKK S
Sbjct: 601 VKREPILELLNSYVVSNYFSPKCPKWVPGQKEWSTKKHS 584

[Close]

BLAST of CmoCh02G001430 vs. NCBI nr
Match: KAG7034947.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1051.2 bits (2717), Expect = 3.4e-303
Identity = 535/625 (85.60%), Postives = 536/625 (85.76%), Query Frame = 0


Query: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60
           MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP
Sbjct: 1   MPGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVP 60

Query: 61  VVSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYS 120
           VVSYENLKPYIDRIANGDASPILCSEP              RKLMPTTEEELKRRSFLYS
Sbjct: 61  VVSYENLKPYIDRIANGDASPILCSEPVSEFLTSSGTSGGERKLMPTTEEELKRRSFLYS 120

Query: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180
           LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY
Sbjct: 121 LLMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDY 180

Query: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240
           TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR
Sbjct: 181 TSPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIR 240

Query: 241 TGTINPRIRDPLVRQSVMNILKPDPVLADLIEFECSKGTWA------------------G 300
           TGTINPRI DPLVRQSVMNILKPDPVLADLIEFECSKGTWA                  G
Sbjct: 241 TGTINPRISDPLVRQSVMNILKPDPVLADLIEFECSKGTWAGIIPRLWPNTKYIDVIVTG 300

Query: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360
           TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD
Sbjct: 301 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLD 360

Query: 361 RKMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSF 420
           RKMSEELVDLVDVELGKEYELVITTYS                                 
Sbjct: 361 RKMSEELVDLVDVELGKEYELVITTYS--------------------------------- 420

Query: 421 PVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSD 480
                                  LYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSID+D
Sbjct: 421 ----------------------GLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDTD 480

Query: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540
           KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF
Sbjct: 481 KVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPDLVF 540

Query: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 594
           ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC
Sbjct: 541 ENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRC 570

[Close]

BLAST of CmoCh02G001430 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 795.8 bits (2054), Expect = 2.4e-230
Identity = 404/642 (62.93%), Postives = 466/642 (72.59%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IE++TT+AD VQRRVL EILSRNA VEYL++ GL+G  DR  FK+I+PVV+YE+++P
Sbjct: 25  LQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYEDIQP 84

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD S +LCS P              RKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPNQTILC
Sbjct: 145 VPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           +DSYQSMY Q LCGLC  ++VLRVG+VFASGFIRAIKFLE HW  LA DIRTGT++  I 
Sbjct: 205 SDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           D  VR++V  ILKPDP LAD +E EC K +W                   GTMSQYIPTL
Sbjct: 265 DSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                   K  +ELVDLVDV+LG+EYELV+TTY+                          
Sbjct: 385 SLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA-------------------------- 444

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         LYRYRVGD+L V+GFKN APQF+F+CRKNV
Sbjct: 445 -----------------------------GLYRYRVGDVLSVAGFKNNAPQFSFICRKNV 504

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+      
Sbjct: 505 VLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLNGNT 564

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 604
            IP  VFE+CCLTIEESLNSVYRQGRVSDKSIGPLEIK++E+GTFDKLMDYAI+ G+SIN
Sbjct: 565 PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISLGASIN 611

[Close]

BLAST of CmoCh02G001430 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 793.5 bits (2048), Expect = 1.2e-229
Identity = 401/642 (62.46%), Postives = 466/642 (72.59%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           LQ IEE+T++ADQVQR+VL EIL+RNA VEYL++  L+G  DR  FKNI+PV++YE+++P
Sbjct: 25  LQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYEDIEP 84

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I+RIANGD SPIL S+P              RKLMPT EEEL RRS LYSLLMPVM+QF
Sbjct: 85  EINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQF 144

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL  GKGMYFLF+KSES TPGGLPARPVLTSYYKSSHFK+RPYD YT+YTSPN+TILC
Sbjct: 145 VPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILC 204

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           +DSYQSMY Q LCGLC  Q+VLRVG+VFASGFIRAIKFLE HW+ L  DIRTGT++  I 
Sbjct: 205 SDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLIT 264

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DP VR++V  ILKP P LAD +EFEC K +W                   GTMSQYIPTL
Sbjct: 265 DPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTL 324

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDR-------- 369
           DYYSNGLPLVCTMYASSECYFGVNL PLC P+EVSYTLIP+MAYFEFLP+ R        
Sbjct: 325 DYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSI 384

Query: 370 --------KMSEELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSM 429
                   K  +ELVDLVDV+LG+EYELV+TTY+                          
Sbjct: 385 NLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA-------------------------- 444

Query: 430 GSLVYSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNV 489
                                         L RYRVGD+LRV+GFKNKAPQF+F+CRKNV
Sbjct: 445 -----------------------------GLCRYRVGDLLRVTGFKNKAPQFSFICRKNV 504

Query: 490 ILSIDSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQKETN 549
           +LSIDSDK DEVELQNAV  A  +L+P++ SL EYTSYADT ++PGHYV++WE+      
Sbjct: 505 VLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLDGNT 564

Query: 550 AIPDLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSIN 604
            IP  VFE+CCL +EES N+VYRQGRVSDKSIGPLEIKI+E GTFDKLMDYAI+ G+SIN
Sbjct: 565 PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASIN 611

[Close]

BLAST of CmoCh02G001430 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 650.6 bits (1677), Expect = 1.3e-186
Identity = 342/634 (53.94%), Postives = 422/634 (66.56%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T + D VQ +VL EIL+RN++ EYL++  LDG +DR  FK+ VPVV+YE+LKP
Sbjct: 24  LKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVTYEDLKP 83

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RI+NGD SPIL S P              RKLMPT EE+L RR  LYSLLMPVMN +
Sbjct: 84  EIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLMPVMNLY 143

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKG+YFLFVKSES T GGLPARPVLTSYYKS HFK RPYD Y  YTSPN+ ILC
Sbjct: 144 VPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSPNEAILC 203

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
           +DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+ +W  LA DI TGT++ RI 
Sbjct: 204 SDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGTLSSRIF 263

Query: 250 DPLVRQSVMNIL-KPDPVLADLIEFECSKGTW------------------AGTMSQYIPT 309
           DP ++  +  IL KPD  LA+ +   CS+  W                   G M+QYIPT
Sbjct: 264 DPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAMAQYIPT 323

Query: 310 LDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLP--------LD 369
           L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP         +
Sbjct: 324 LEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPHNHDGDGAAE 383

Query: 370 RKMSE-ELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYS 429
             + E  LV+L +VE+GKEYELVITTY+                                
Sbjct: 384 ASLDETSLVELANVEVGKEYELVITTYA-------------------------------- 443

Query: 430 FPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDS 489
                                   LYRYRVGDILRV+GF N APQF F+ RKNV+LS++S
Sbjct: 444 -----------------------GLYRYRVGDILRVTGFHNSAPQFKFIRRKNVLLSVES 503

Query: 490 DKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWE-ISQKETNAI-PD 549
           DK DE ELQ AV  AS+      T ++EYTSYA+T T+PGHYVIYWE + + ++NA+  +
Sbjct: 504 DKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVIYWELLGRDQSNALMSE 563

Query: 550 LVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKT 600
            V   CCL +EESLNSVYRQ RV+DKSIGPLEI+++ NGTF++LMDYAI+ G+SINQYK 
Sbjct: 564 EVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEELMDYAISRGASINQYKV 602

[Close]

BLAST of CmoCh02G001430 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 646.0 bits (1665), Expect = 3.1e-185
Identity = 338/624 (54.17%), Postives = 409/624 (65.54%), Query Frame = 0


Query: 10  LQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPVVSYENLKP 69
           L+ IEE+T +AD VQ  +L EIL+RNA  EYL++  L G  DR  FK  +PV++YE+L+P
Sbjct: 23  LRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVITYEDLQP 82

Query: 70  YIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSLLMPVMNQF 129
            I RIA+GD SPIL + P              RKLMPT +EEL RR  LYSLLMPVMN +
Sbjct: 83  EIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLY 142

Query: 130 IPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYTSPNQTILC 189
           +PGL+KGKGMYFLFVKSE+ TPGGLPARPVLTSYYKS HF+ RPYD Y  YTSPN+ ILC
Sbjct: 143 VPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEAILC 202

Query: 190 TDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRTGTINPRIR 249
            DS+QSMY Q LCGL     VLRVG+VFASG +RAI+FL+ HW   A+DI  G ++  I 
Sbjct: 203 PDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDSEIT 262

Query: 250 DPLVRQSVMNILKPDPVLADLIEFECSKGTW------------------AGTMSQYIPTL 309
           DP +RQ +  ILKPDPVLA+ I  EC    W                   G M+QYIPTL
Sbjct: 263 DPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQYIPTL 322

Query: 310 DYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPLDRKMSEELVD 369
           +YYS GLP+ CTMYASSECYFG+NLNP+  P+EVSYT++P MAYFEF+PL      + V+
Sbjct: 323 EYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG---GTKAVE 382

Query: 370 LVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLVYSFPVYDSVYSF 429
           LVDV +GKEYELV+TTY+                                          
Sbjct: 383 LVDVNIGKEYELVVTTYA------------------------------------------ 442

Query: 430 PVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSIDSDKVDEVELQN 489
                         L RYRVGDILRV+GF N APQF+FV RKNV+LSIDSDK DE ELQ 
Sbjct: 443 -------------GLCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQK 502

Query: 490 AVMEASKNL-IPYETSLLEYTSYADTHTVPGHYVIYWEISQKETNAIPD-LVFENCCLTI 549
           AV  AS  L     + + EYTSYADT T+PGHYV+YWE+  ++    P       CCL +
Sbjct: 503 AVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGM 562

Query: 550 EESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYKTPRCVKHEPIL 600
           EESLNSVYRQ RV+D S+GPLEI+++ NGTF++LMDYAI+ G+SINQYK PRCV   PI+
Sbjct: 563 EESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIV 588

[Close]

BLAST of CmoCh02G001430 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 642.1 bits (1655), Expect = 4.5e-184
Identity = 344/635 (54.17%), Postives = 419/635 (65.98%), Query Frame = 0


Query: 2   PGKDEGALLQHIEEITTHADQVQRRVLTEILSRNAHVEYLQQQGLDGCMDRMAFKNIVPV 61
           P   +   L+ IEE+T + D VQ++V+ EILSRN+  EYL++ GL G  DR  FK  VPV
Sbjct: 15  PSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPV 74

Query: 62  VSYENLKPYIDRIANGDASPILCSEP--------------RKLMPTTEEELKRRSFLYSL 121
           V Y++LKP I RIANGD S IL S P              RKLMPT +E++ RR  LYSL
Sbjct: 75  VIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSL 134

Query: 122 LMPVMNQFIPGLNKGKGMYFLFVKSESTTPGGLPARPVLTSYYKSSHFKDRPYDLYTDYT 181
           LMPVMN ++PGL+KGK +YFLFVK+ES TPGGLPARPVLTSYYKS  FK RP D Y  YT
Sbjct: 135 LMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYT 194

Query: 182 SPNQTILCTDSYQSMYCQFLCGLCLSQQVLRVGSVFASGFIRAIKFLETHWVLLANDIRT 241
           SPN+ ILC DS QSMY Q LCGL +  +VLR+G+VFASG +RAI FL+T+W  LA+DI T
Sbjct: 195 SPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDIST 254

Query: 242 GTINPRIRDPLVRQSVMNIL-KPDPVLADLIEFECSK-GTW------------------A 301
           GT++ RI DP +++S+  IL KPD  LAD I   C +  +W                   
Sbjct: 255 GTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVT 314

Query: 302 GTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCNPNEVSYTLIPTMAYFEFLPL 361
           G M+QYIP L+YYS GLP+ CTMYASSE YFG+NL P+C P+EVSYT++P MAYFEFLP 
Sbjct: 315 GAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLPH 374

Query: 362 DRKMSE--ELVDLVDVELGKEYELVITTYSENPWVCRLFIAYSIVITVRHNCLDSMGSLV 421
               +E  ELV+L DVE+GKEYELVITTY+                              
Sbjct: 375 HEVPTEKSELVELADVEVGKEYELVITTYA------------------------------ 434

Query: 422 YSFPVYDSVYSFPVYDSVYSFPVYDRLYRYRVGDILRVSGFKNKAPQFNFVCRKNVILSI 481
                                     L RYRVGDIL+V+GF N APQF FV RKNV+LSI
Sbjct: 435 -------------------------GLNRYRVGDILQVTGFYNSAPQFKFVRRKNVLLSI 494

Query: 482 DSDKVDEVELQNAVMEASKNLIPYETSLLEYTSYADTHTVPGHYVIYWEISQK-ETNAIP 541
           +SDK DE ELQ+AV  AS  L    T ++EYTSYA+T T+PGHYVIYWE+  K +TN   
Sbjct: 495 ESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQTNPPN 554

Query: 542 DLVFENCCLTIEESLNSVYRQGRVSDKSIGPLEIKIIENGTFDKLMDYAINHGSSINQYK 600
           D V   CCL +EESLNSVYRQ RV+DKSIGPLEI++++NGTF++LMDYAI+ G+SINQYK
Sbjct: 555 DEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGASINQYK 594

The following BLAST results are available for this feature:

BLAST of CmoCh02G001430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	3.4e-229	62.93	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	1.7e-228	62.46	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	6.0e-194	54.65	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
A3BLS0	8.5e-188	54.37	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]
Q0D4Z6	8.5e-188	54.37	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3...	[more]

BLAST of CmoCh02G001430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G8Z2	0.0e+00	86.23	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1I4E6	1.1e-307	84.66	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1CG47	9.5e-259	71.25	indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charant...	[more]
A0A1U7YQ53	4.3e-235	64.09	indole-3-acetic acid-amido synthetase GH3.6 OS=Nelumbo nucifera OX=4432 GN=LOC10...	[more]
A0A7J7C2B3	1.1e-233	63.66	Putative Indole-3-acetic acid-amido synthetase GH3.6 OS=Tripterygium wilfordii O...	[more]

BLAST of CmoCh02G001430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948165.1	0.0e+00	86.23	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
KAG6604833.1	0.0e+00	85.76	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023533206.1	1.2e-308	84.66	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970970.1	2.3e-307	84.66	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima]	[more]
KAG7034947.1	3.4e-303	85.60	Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma sub...	[more]

BLAST of CmoCh02G001430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	2.4e-230	62.93	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	1.2e-229	62.46	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	1.3e-186	53.94	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	3.1e-185	54.17	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	4.5e-184	54.17	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 412..571 e-value: 2.7E-56 score: 191.5 coord: 13..355 e-value: 8.9E-103 score: 344.9
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 8..355
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 408..601
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 8..355 coord: 408..601

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G001430.1	CmoCh02G001430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity