CmoCh02G001320 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh02G001320
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	SPARK domain-containing protein
Location	Cmo_Chr02: 669214 .. 670506 (+)
RNA-Seq Expression	CmoCh02G001320
Synteny	CmoCh02G001320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCTCTCTGATGGTTGTAGTTTCATCAATCCTTATGATCTTATCGACTCTGTTCCAAGTTTCTGTTGCTGGCGTAACAGGGGAATCCTCTGTTCCGGCCATCCCAGTAGAAGCTGAAACTCAAGTCTGTCGGCTGGACCTATCCGACGAGCTATTCGGGGGAGTGAGCCAAGCCTGCAATGGGAACCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCGGCATGGCTCTTTGCAGCTCATGCCAGGTCGGCGTTAGAAGTGTCGGCTCCAGCGCCAACAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTGGAGTCATTACAAAACTCGTTGGTGAAGCGAAACATCAGAATTCCGCAGCCCAATTCCAGCTGCGACGCTGTATTGTGCTTCTGTGGAATTCGTCTCCACCAGATAAGTTCCTTGTCTTGTCCAGCAGCATTCAATCTCTCCGGCTTCCACAACGCCACCCCAACGTCGGCAGTGCGTGATCTGGAGAAGAATTGCCGCAACGCATCCTACTCCGGTTGCACCAAATGCCTCGGCGCACTCCAAAAGGTACTGCAAAACAACTTAACCGTAACGATTCTTATCTCATCGACTCCCACATTGAAATGGGTAGCTGAAAAATGGGATCTTTTCGCCTTTTTAGCTCCTTAATTTATGTTTCCGATCAGGTGAAGGGAGCTAGAGACACCAGCACCACCGAAAGAGCAACCAAAATGTTCAACAGAGACTGCCAACTGATGGGGCTAACATGGCTGCTCGCTCGCAACAAAACGGCGTACATTCCAACAGTTTCAGCGGTTCTAAGAGCCATAATGTACAGTGCCCACCCGCCTCACGATTCAAAGTGCAGCCCAGATCAAGAAAACATGCCACTGGCCGTTGATTCCCTTCAATTTGAGAACACACAGTCCTCCACTTCATCACCCTCGACCTTCATGTATCCATTCTTGCCCTTAACAGCAGTGCCCTTCATCTGGTTCTTGACACATCCGTAATATGAGGTTCCGTTGGGCCGTAGTTTCTTGGATTATTTTGGAGGGAAAGACCATGTCGTGTCTGTCACATGAAAGCTGTTGATTTTGTGTCTTTTTTCTTAACCCTACAGTAAAATTGGGTTTGGTGGGACAGATCTGCTTCAGTTTTGTTTTCTTTTACAGCAGTAAATATTCCAGTTTGAAGTGCATTGAATTCTGTGAAACGTTTCAAATATTCTCACAAAAATGGGGTCTCTCCACGAGTCACAGACAAACGTTTAAAGATATAGAA

mRNA sequence

ATGGTCTCTCTGATGGTTGTAGTTTCATCAATCCTTATGATCTTATCGACTCTGTTCCAAGTTTCTGTTGCTGGCGTAACAGGGGAATCCTCTGTTCCGGCCATCCCAGTAGAAGCTGAAACTCAAGTCTGTCGGCTGGACCTATCCGACGAGCTATTCGGGGGAGTGAGCCAAGCCTGCAATGGGAACCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCGGCATGGCTCTTTGCAGCTCATGCCAGGTCGGCGTTAGAAGTGTCGGCTCCAGCGCCAACAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTGGAGTCATTACAAAACTCGTTGGTGAAGCGAAACATCAGAATTCCGCAGCCCAATTCCAGCTGCGACGCTGTATTGTGCTTCTGTGGAATTCGTCTCCACCAGATAAGTTCCTTGTCTTGTCCAGCAGCATTCAATCTCTCCGGCTTCCACAACGCCACCCCAACGTCGGCAGTGCGTGATCTGGAGAAGAATTGCCGCAACGCATCCTACTCCGGTTGCACCAAATGCCTCGGCGCACTCCAAAAGGTACTGCAAAACAACTTAACCGTAACGATTCTTATCTCATCGACTCCCACATTGAAATGGGTGAAGGGAGCTAGAGACACCAGCACCACCGAAAGAGCAACCAAAATGTTCAACAGAGACTGCCAACTGATGGGGCTAACATGGCTGCTCGCTCGCAACAAAACGGCGTACATTCCAACAGTTTCAGCGGTTCTAAGAGCCATAATGTACAGTGCCCACCCGCCTCACGATTCAAAGTGCAGCCCAGATCAAGAAAACATGCCACTGGCCGTTGATTCCCTTCAATTTGAGAACACACAGTCCTCCACTTCATCACCCTCGACCTTCATGTATCCATTCTTGCCCTTAACAGCAGTGCCCTTCATCTGGTTCTTGACACATCCGTAATATGAGGTTCCGTTGGGCCGTAGTTTCTTGGATTATTTTGGAGGGAAAGACCATGTCGTGTCTGTCACATGAAAGCTGTTGATTTTGTGTCTTTTTTCTTAACCCTACAGTAAAATTGGGTTTGGTGGGACAGATCTGCTTCAGTTTTGTTTTCTTTTACAGCAGTAAATATTCCAGTTTGAAGTGCATTGAATTCTGTGAAACGTTTCAAATATTCTCACAAAAATGGGGTCTCTCCACGAGTCACAGACAAACGTTTAAAGATATAGAA

Coding sequence (CDS)

ATGGTCTCTCTGATGGTTGTAGTTTCATCAATCCTTATGATCTTATCGACTCTGTTCCAAGTTTCTGTTGCTGGCGTAACAGGGGAATCCTCTGTTCCGGCCATCCCAGTAGAAGCTGAAACTCAAGTCTGTCGGCTGGACCTATCCGACGAGCTATTCGGGGGAGTGAGCCAAGCCTGCAATGGGAACCTAGACCGGAGCCGGTGCTGTCCAGTGCTAGCGGCATGGCTCTTTGCAGCTCATGCCAGGTCGGCGTTAGAAGTGTCGGCTCCAGCGCCAACAGCCGAAATAGATCTGCCATTGATGCCAGACGATTCCCAGAAATGCGTGGAGTCATTACAAAACTCGTTGGTGAAGCGAAACATCAGAATTCCGCAGCCCAATTCCAGCTGCGACGCTGTATTGTGCTTCTGTGGAATTCGTCTCCACCAGATAAGTTCCTTGTCTTGTCCAGCAGCATTCAATCTCTCCGGCTTCCACAACGCCACCCCAACGTCGGCAGTGCGTGATCTGGAGAAGAATTGCCGCAACGCATCCTACTCCGGTTGCACCAAATGCCTCGGCGCACTCCAAAAGGTACTGCAAAACAACTTAACCGTAACGATTCTTATCTCATCGACTCCCACATTGAAATGGGTGAAGGGAGCTAGAGACACCAGCACCACCGAAAGAGCAACCAAAATGTTCAACAGAGACTGCCAACTGATGGGGCTAACATGGCTGCTCGCTCGCAACAAAACGGCGTACATTCCAACAGTTTCAGCGGTTCTAAGAGCCATAATGTACAGTGCCCACCCGCCTCACGATTCAAAGTGCAGCCCAGATCAAGAAAACATGCCACTGGCCGTTGATTCCCTTCAATTTGAGAACACACAGTCCTCCACTTCATCACCCTCGACCTTCATGTATCCATTCTTGCCCTTAACAGCAGTGCCCTTCATCTGGTTCTTGACACATCCGTAA

Protein sequence

MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYSGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFLTHP

Homology

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BLAST of CmoCh02G001320 vs. ExPASy Swiss-Prot
Match: Q9SUC9 (Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 GN=At4g28100 PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 2.5e-106
Identity = 205/315 (65.08%), Postives = 243/315 (77.14%), Query Frame = 0


Query: 11  ILMILSTLFQVSVAGVTGE----SSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDR 70
           IL++ S LF   ++ +  E    ++VPA PVE + Q CRLDLS+ELFGGV++AC  NLDR
Sbjct: 9   ILLLCSLLFSTVLSNLLVEPVQPNTVPAFPVETQAQSCRLDLSNELFGGVNEACGRNLDR 68

Query: 71  SRCCPVLAAWLFAAHARSALEVSAPAPTAEI---DLPLMPDDSQKCVESLQNSLVKRNIR 130
           SRCCPVLAAWLFAAHARSAL++ APAPT E    D P+ PDDSQKCV +LQ++L+ + I+
Sbjct: 69  SRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVNTLQSALLTKQIK 128

Query: 131 IPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNL-SGFHNATPTSAVRDLEKNCRNASYSG 190
           IPQPNSSCDA+LCFCGIRLHQISSLSCPAAFN+ SGF NATPT+AV++LEK CRN+SYSG
Sbjct: 129 IPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNATPTAAVKNLEKECRNSSYSG 188

Query: 191 CTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTWLL 250
           CT+CLGALQK+                   V+G    +TTER TKM ++DCQLMGLTWLL
Sbjct: 189 CTRCLGALQKLK------------------VRGGNKKTTTERGTKMMSKDCQLMGLTWLL 248

Query: 251 ARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFM 310
           ARNKTAYIPTVSAVLRAIMYS HPPH +KCSPDQENMPLAVDSLQF+ + SS SS    +
Sbjct: 249 ARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSS-SSHLFGV 304

Query: 311 YPFLPLTAVPFIWFL 318
            PFLPL    F++ L
Sbjct: 309 LPFLPLVLCIFLFLL 304

[Close]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL
Match: A0A6J1G647 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=3662 GN=LOC111451182 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.3e-162
Identity = 300/320 (93.75%), Postives = 300/320 (93.75%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 106 MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 165

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 166 NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 225

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY
Sbjct: 226 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 285

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDTSTTERATKMFNRDCQLMGLTW
Sbjct: 286 SGCTKCLGALQK--------------------VKGARDTSTTERATKMFNRDCQLMGLTW 345

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST
Sbjct: 346 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 405

Query: 301 FMYPFLPLTAVPFIWFLTHP 321
           FMYPFLPLTAVPFIWFLTHP
Sbjct: 406 FMYPFLPLTAVPFIWFLTHP 405

[Close]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL
Match: A0A6J1HZY7 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 GN=LOC111469629 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 4.1e-160
Identity = 297/320 (92.81%), Postives = 298/320 (93.12%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPT AVRDLEKNCRNASY
Sbjct: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNASY 180

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDT+TTERATKMFNRDCQLMGLTW
Sbjct: 181 SGCTKCLGALQK--------------------VKGARDTNTTERATKMFNRDCQLMGLTW 240

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST
Sbjct: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300

Query: 301 FMYPFLPLTAVPFIWFLTHP 321
           FMYPFLPLTAV FIWFLTHP
Sbjct: 301 FMYPFLPLTAVLFIWFLTHP 300

[Close]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL
Match: A0A0A0KAY3 (SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 8.6e-126
Identity = 244/317 (76.97%), Postives = 265/317 (83.60%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVA-GVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60
           M+S++ + +  L+I +TLF +SVA  V G+ SVPA PVE E Q CRLDLSDELFGGVSQA
Sbjct: 1   MISILAIAT--LVISTTLFPISVAVSVRGQYSVPAFPVETENQACRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVR 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT+AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTAAVRDLETNCRNAS 180

Query: 181 YSGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLT 240
           YSGCTKCLGALQK                    VKGA D STT RA KMFNRDCQLMGLT
Sbjct: 181 YSGCTKCLGALQK--------------------VKGAGDRSTTARARKMFNRDCQLMGLT 240

Query: 241 WLLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQS-STSSP 300
           WLLARNKTAYIPTVSAVLRAIMYSAHPPH+S CSPDQENMPLAVDSLQF++  S S S P
Sbjct: 241 WLLARNKTAYIPTVSAVLRAIMYSAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP 295

Query: 301 ST-FMYPFLPLTAVPFI 315
           S+ F++ FL L   PF+
Sbjct: 301 SSIFIFSFLRLLLTPFV 295

[Close]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL
Match: A0A5D3B9U0 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00450 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.5e-125
Identity = 242/316 (76.58%), Postives = 261/316 (82.59%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVT-GESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60
           M+S++ + +   +I +TLF +SVA V  G+ SVPA PVE E Q CRLDLSDELFGGVSQA
Sbjct: 1   MISILAIAT--FLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVR 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT+AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNAS 180

Query: 181 YSGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLT 240
           YSGCTKCLGALQK                    VKGA D STT RA KMFNRDCQLMGLT
Sbjct: 181 YSGCTKCLGALQK--------------------VKGAGDRSTTARARKMFNRDCQLMGLT 240

Query: 241 WLLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQS-STSSP 300
           WLLARNKTAYIPTVSAVLRAIMYSAH PH+S CSPDQENMPLAVDSLQF++  S S S P
Sbjct: 241 WLLARNKTAYIPTVSAVLRAIMYSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP 294

Query: 301 STFMYPFLPLTAVPFI 315
           S FM+ F  L   PF+
Sbjct: 301 SIFMFSFFRLVLTPFV 294

[Close]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL
Match: A0A1S3BJT3 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=LOC103490480 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.5e-125
Identity = 242/316 (76.58%), Postives = 261/316 (82.59%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVT-GESSVPAIPVEAETQVCRLDLSDELFGGVSQA 60
           M+S++ + +   +I +TLF +SVA V  G+ SVPA PVE E Q CRLDLSDELFGGVSQA
Sbjct: 1   MISILAIAT--FLISTTLFPISVAVVVRGQFSVPAFPVETENQACRLDLSDELFGGVSQA 60

Query: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVK 120
           CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAP+AEIDLPLMPDDSQKCVESLQ+SLV+
Sbjct: 61  CNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDDSQKCVESLQSSLVR 120

Query: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNAS 180
           RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+PT+AVRDLE NCRNAS
Sbjct: 121 RNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASPTTAVRDLETNCRNAS 180

Query: 181 YSGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLT 240
           YSGCTKCLGALQK                    VKGA D STT RA KMFNRDCQLMGLT
Sbjct: 181 YSGCTKCLGALQK--------------------VKGAGDRSTTARARKMFNRDCQLMGLT 240

Query: 241 WLLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQS-STSSP 300
           WLLARNKTAYIPTVSAVLRAIMYSAH PH+S CSPDQENMPLAVDSLQF++  S S S P
Sbjct: 241 WLLARNKTAYIPTVSAVLRAIMYSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQP 294

Query: 301 STFMYPFLPLTAVPFI 315
           S FM+ F  L   PF+
Sbjct: 301 SIFMFSFFRLVLTPFV 294

[Close]

BLAST of CmoCh02G001320 vs. NCBI nr
Match: XP_022947263.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 6.9e-162
Identity = 300/320 (93.75%), Postives = 300/320 (93.75%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 106 MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 165

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 166 NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 225

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY
Sbjct: 226 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 285

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDTSTTERATKMFNRDCQLMGLTW
Sbjct: 286 SGCTKCLGALQK--------------------VKGARDTSTTERATKMFNRDCQLMGLTW 345

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST
Sbjct: 346 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 405

Query: 301 FMYPFLPLTAVPFIWFLTHP 321
           FMYPFLPLTAVPFIWFLTHP
Sbjct: 406 FMYPFLPLTAVPFIWFLTHP 405

[Close]

BLAST of CmoCh02G001320 vs. NCBI nr
Match: XP_023532424.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 575.5 bits (1482), Expect = 2.9e-160
Identity = 297/320 (92.81%), Postives = 298/320 (93.12%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPT AVRDLEKNCRNASY
Sbjct: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNASY 180

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDTSTTERATKMFNRDCQLMGLTW
Sbjct: 181 SGCTKCLGALQK--------------------VKGARDTSTTERATKMFNRDCQLMGLTW 240

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQ +NTQSSTSSPST
Sbjct: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQLDNTQSSTSSPST 300

Query: 301 FMYPFLPLTAVPFIWFLTHP 321
           FMYPFLPLTAVPFIWFLTHP
Sbjct: 301 FMYPFLPLTAVPFIWFLTHP 300

[Close]

BLAST of CmoCh02G001320 vs. NCBI nr
Match: XP_022970732.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita maxima])

HSP 1 Score: 573.9 bits (1478), Expect = 8.4e-160
Identity = 297/320 (92.81%), Postives = 298/320 (93.12%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPT AVRDLEKNCRNASY
Sbjct: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTPAVRDLEKNCRNASY 180

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDT+TTERATKMFNRDCQLMGLTW
Sbjct: 181 SGCTKCLGALQK--------------------VKGARDTNTTERATKMFNRDCQLMGLTW 240

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST
Sbjct: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300

Query: 301 FMYPFLPLTAVPFIWFLTHP 321
           FMYPFLPLTAV FIWFLTHP
Sbjct: 301 FMYPFLPLTAVLFIWFLTHP 300

[Close]

BLAST of CmoCh02G001320 vs. NCBI nr
Match: KAG7034939.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.5 bits (1456), Expect = 3.0e-157
Identity = 294/314 (93.63%), Postives = 294/314 (93.63%), Query Frame = 0


Query: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60
           MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC
Sbjct: 1   MVSLMVVVSSILMILSTLFQVSVAGVTGESSVPAIPVEAETQVCRLDLSDELFGGVSQAC 60

Query: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120
           NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR
Sbjct: 61  NGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKR 120

Query: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180
           NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY
Sbjct: 121 NIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASY 180

Query: 181 SGCTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTW 240
           SGCTKCLGALQK                    VKGARDTSTTERATKMFNRDCQLMGLTW
Sbjct: 181 SGCTKCLGALQK--------------------VKGARDTSTTERATKMFNRDCQLMGLTW 240

Query: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 300
           LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST
Sbjct: 241 LLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPST 294

Query: 301 FMYPFLPLTAVPFI 315
           FMYPFLPLTAVPFI
Sbjct: 301 FMYPFLPLTAVPFI 294

[Close]

BLAST of CmoCh02G001320 vs. NCBI nr
Match: KAG6604823.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 273/293 (93.17%), Postives = 273/293 (93.17%), Query Frame = 0


Query: 28  GESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALE 87
           GESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALE
Sbjct: 148 GESSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALE 207

Query: 88  VSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISS 147
           VSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISS
Sbjct: 208 VSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISS 267

Query: 148 LSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYSGCTKCLGALQKVLQNNLTVTILISST 207
           LSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYSGCTKCLGALQK               
Sbjct: 268 LSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYSGCTKCLGALQK--------------- 327

Query: 208 PTLKWVKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPP 267
                VKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPP
Sbjct: 328 -----VKGARDTSTTERATKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPP 387

Query: 268 HDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFLTHP 321
           HDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFLTHP
Sbjct: 388 HDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPFLPLTAVPFIWFLTHP 420

[Close]

BLAST of CmoCh02G001320 vs. TAIR 10
Match: AT4G28100.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G18050.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 386.7 bits (992), Expect = 1.8e-107
Identity = 205/315 (65.08%), Postives = 243/315 (77.14%), Query Frame = 0


Query: 11  ILMILSTLFQVSVAGVTGE----SSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDR 70
           IL++ S LF   ++ +  E    ++VPA PVE + Q CRLDLS+ELFGGV++AC  NLDR
Sbjct: 9   ILLLCSLLFSTVLSNLLVEPVQPNTVPAFPVETQAQSCRLDLSNELFGGVNEACGRNLDR 68

Query: 71  SRCCPVLAAWLFAAHARSALEVSAPAPTAEI---DLPLMPDDSQKCVESLQNSLVKRNIR 130
           SRCCPVLAAWLFAAHARSAL++ APAPT E    D P+ PDDSQKCV +LQ++L+ + I+
Sbjct: 69  SRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVNTLQSALLTKQIK 128

Query: 131 IPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNL-SGFHNATPTSAVRDLEKNCRNASYSG 190
           IPQPNSSCDA+LCFCGIRLHQISSLSCPAAFN+ SGF NATPT+AV++LEK CRN+SYSG
Sbjct: 129 IPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNATPTAAVKNLEKECRNSSYSG 188

Query: 191 CTKCLGALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTERATKMFNRDCQLMGLTWLL 250
           CT+CLGALQK+                   V+G    +TTER TKM ++DCQLMGLTWLL
Sbjct: 189 CTRCLGALQKLK------------------VRGGNKKTTTERGTKMMSKDCQLMGLTWLL 248

Query: 251 ARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFM 310
           ARNKTAYIPTVSAVLRAIMYS HPPH +KCSPDQENMPLAVDSLQF+ + SS SS    +
Sbjct: 249 ARNKTAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSS-SSHLFGV 304

Query: 311 YPFLPLTAVPFIWFL 318
            PFLPL    F++ L
Sbjct: 309 LPFLPLVLCIFLFLL 304

[Close]

BLAST of CmoCh02G001320 vs. TAIR 10
Match: AT3G18050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G28100.1); Has 67 Blast hits to 66 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 201.1 bits (510), Expect = 1.4e-51
Identity = 118/299 (39.46%), Postives = 166/299 (55.52%), Query Frame = 0


Query: 30  SSVPAIPVEAETQVCRLDLSDELFGGVSQACNG-NLDRSRCCPVLAAWLFAAH------- 89
           +++PA P +++   C LDL ++LF G+  AC+G  L + +CCPVL AWL++A+       
Sbjct: 41  ATIPAFPEQSDFSGCPLDLPEDLFHGIKSACSGKKLHKGKCCPVLGAWLYSAYSTTALSR 100

Query: 90  -----ARSALEVSAPAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLC 149
                AR++   SA   T E D+PL+PDDS+ CV+ L  SL +R I + +PN +CD V C
Sbjct: 101 SISAAARNSSSSSAAVTTPEEDMPLLPDDSETCVDGLGKSLRQRGIELTRPNETCDVVYC 160

Query: 150 FCGIRLHQISSLSCPAAFNLSGFHNATPTSAVRDLEKNCRNASYS---------GCTKCL 209
           +CGIRLH    LSC  AF ++          V  LE +C + S++         GC KCL
Sbjct: 161 YCGIRLH---PLSCSEAFRVNDEGRLVGDERVDRLETDCLSGSHNNADGFSPLLGCNKCL 220

Query: 210 GALQKVLQNNLTVTILISSTPTLKWVKGARDTSTTE--RATKMFNRDCQLMGLTWLLARN 269
            +L K+                 K   G R+ S  +  R  KM N+DC LMGLTWLLA+N
Sbjct: 221 NSLYKL---------------NPKKTSGTRNPSKEDRNRTAKMHNKDCVLMGLTWLLAKN 280

Query: 270 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYP 305
           +TAY PTV++VLRA+M + H      C+   + MPLAVDS +F N      SP++  YP
Sbjct: 281 RTAYFPTVTSVLRAVMLN-HDGVPRSCALGSDGMPLAVDSSEFSN-----GSPTSLQYP 315

The following BLAST results are available for this feature:

BLAST of CmoCh02G001320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUC9	2.5e-106	65.08	Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 G...	[more]

BLAST of CmoCh02G001320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G647	3.3e-162	93.75	uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=366...	[more]
A0A6J1HZY7	4.1e-160	92.81	uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A0A0KAY3	8.6e-126	76.97	SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 ...	[more]
A0A5D3B9U0	2.5e-125	76.58	Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BJT3	2.5e-125	76.58	uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=L...	[more]

BLAST of CmoCh02G001320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947263.1	6.9e-162	93.75	uncharacterized GPI-anchored protein At4g28100-like [Cucurbita moschata]	[more]
XP_023532424.1	2.9e-160	92.81	uncharacterized GPI-anchored protein At4g28100-like [Cucurbita pepo subsp. pepo]	[more]
XP_022970732.1	8.4e-160	92.81	uncharacterized GPI-anchored protein At4g28100-like [Cucurbita maxima]	[more]
KAG7034939.1	3.0e-157	93.63	putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6604823.1	1.2e-150	93.17	putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmoCh02G001320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G28100.1	1.8e-107	65.08	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G18050.1	1.4e-51	39.46	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR043891	SPARK domain	PFAM	PF19160	SPARK	coord: 41..201 e-value: 1.4E-18 score: 67.8
None	No IPR available	PANTHER	PTHR34056:SF3	OS07G0557700 PROTEIN	coord: 23..308
IPR040376	At4g28100-like	PANTHER	PTHR34056	GPI-ANCHORED PROTEIN	coord: 23..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh02G001320.1	CmoCh02G001320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane