CmoCh02G001310 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh02G001310
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionvesicle-associated protein 4-2-like
LocationCmo_Chr02: 662245 .. 665212 (-)
RNA-Seq ExpressionCmoCh02G001310
SyntenyCmoCh02G001310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTTTCTTAATAAAAACAGAGCAACTGTCGGGCAGGGCAGGCGTTGCCAACTAAAATGCAAGGATGATTCGAAGAACAGTACAATGGAACCGTATTCCTTGTCGGGTATGACATAAATAACCGCATCTACAGCTTCAGCCCATCAAACGATCCAAATAGAAAAAACCCAAAATAGAAATGATCCAAACCGAAGTTGGAAGAGTTGTTTTCGAGAGAAGCGAGTAAGCAAAACCCCTTTTGTGATAAATCCCAAATCCATAAGCTTCTCGCCAAGCAACCAAGGAATCTACCGACTTCAATGGCTGTCGCTGGTCAGAAATCAGACCTCGATGGCAAGGTTTGGAGCCTTTGTAGTATACCCTTTTGGCAAAATGGCAATGTCTCCTCGTCTTCTGGTTCTTCGTCGACTGCTATTCATCGCGTTAATGAGCAAAGTAATGGTCATAAGCCAACAGAGAACTCGAGCTCCTATCCTTCTGGCAAGGTTTCGTTCATTGCGAAATCTCTACTTCCTACCAGAAGAAGGCTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATGTATGTACCCTATTTTCTCATCTGGGTCGCCATTTTTTTGGGTTCATAATGAGATTTTGTTGCCTTTTTCGTTTTGTTCTTACAACTGTGGTCGTCCTCCCCTGTTCTCTGGCTTGATGATTACTAAATCACTCCCTAAAATCGATCTGTTCTTGCTTGGGTTTTGAGAATGTTTGTCATTTTTGTTCATCTTTTCAGATTGCTTCCACTTTTTTTGGTCGAACAGCCAAGTTTGTTTAGTTGTCTGATGCCCTTGAATTCCTCTCAGCTTGTGTTTTGTTTTCATTGTCTTTCTCTTAAAGATGAACCTGGGAAGCAGGTAAAGAGTGCTATTGAGATACAGAATACGAGCAAGAATCATGTGGCTTTCAAAGTATGAATCATTTCCCTCACGAACTTTTATCAGTTTTAAGCCTACCCAATGCAGCCTTCTTACTTCTTACCCCCTTGAATTGTTCTAGTTTCAAACGACCGCGCCGAAGAGCTGCTACATGCGTCCTCCTGGCGGTATACTTGCTCCTGGTGAAAGTATCATCGCAACGGGTAAGACATTCATCTTAGTTTTGAGCTGCATTCCTTGAACTGAACATTACAAGTATGAACAGCATTTGAAGTTTTTCTGAGGTTCTTGTATCTGTGACTTTGCTTCAGTGTTCAAGTTTGTGGAGCCACTAGAGAACAATGAGAAGCCTGTGGATCAAAAGAGGAGGGTTAAGTTTAAGATTGTGAGCTTGAAAGTGAAGGGTAACATGGACTATGTGCCAGAGCTGGTATGTCTTGCAGCCTTAACTTCTGAATTTACTATGCTTTTCCATGCTTTGAGTTTGTGACCAATGCTTATATCATTATTGTGCAAGAATGTGTAACAATCCAAGCCTACCGCTAGCGGATACTGTCTTCTTTAAACTTTCCCTTCCGAACTTCTCCTTAAGGTTTTAAAACGCGTCTACTATGGAGAGGATCTAGCCCTACTCCGACCCGTGTCTTGCACCTTCTGGAACCTGGCTAGACCCCCTAAATAGTAGACGCGTTTTAAAATCTTGAGGGAAAGCCCAAAGAACAATATCTGCGAGTAGTAGGCTTGGGATGTTACAAATGGTATCAAAGTCAATCACCGAACGGTGCGAAACACTGGTCAGAGTAGGGCTAGACCCTCTCCAACCAATGTGCGATCTCACAATCCACCCCATTGGGAGCCAGCGTCTTCACTGACACATTGCCCGGTATCTGGCTTTGATACTATTTGTAATAGTTCAAGCCCACCGCTAGTAGATATTGTTCTCTTTTGGGCTTCCTCTCAAGGTTTTAAAACGGTCTACTAGGGAGAAGTTTTTACACCCTTATAAGGAATGGTTCGTTCCCCTCTCCAACCAATGTGGGATCTCACGTTAACAAACCATAACGCACATCTTCAAGTGAAAGACCAATTATAATTCACGCTAAGAAGAAGTTTCTGATGGTAATTTCAAATAATGGCATTTTACAGTTTGATGAACAAAGAGAACATGTAGCAGTTGAGCAAATACTGCAAGTTGTTTTTCTTGACCCGCAGCATTCTAATCCTGTAAGTATTAGTAGTAAGAGGAGAGAAACACACTAAGTATGGTCAAGACTATCAATCTCCTTATTGGTTTGGATTCTTCTCCTGTTTCAGGCTTTGGAGAAACTCTTCCGACAACTAGCTGTGGCCGAGGCTGCAACTGAGGTGCGTCAAAAGCCAGCAGAAGAAACAGACCCGAAAGTCGTTGGTGAAGGACTCGTCATCGACGAATGGGTGAGACAACGAAACTGTTCTCAGCATCCTCTTCAAACCTTATTAAGCTTTGCATTCTCTGACATGCTATGGTTGTTCTTCATTTGTAGAAAGAAAGAAGGGAAAGATACCTAGCTAAGCAACAGGTTGGAGGTGTGGATTTGGTGTGAAATCGAGCTGGTCATGTTATGAAAGTTGGAATCACATTCATGTGTGAACCTTTCTTTGAGGCATTCGTCGTCGAAGAGCAGTCATCAACATCGACATTAGCAATCAAAAGCGAATGGCTTAACGACTTGATTTGTGATGGCGTTTCATGGTCTTGTGTTTGTTTGATTGATTGGGTTGCTGTTTCTGTTGGAGTTTGAAGGTTTTGAGGTAAATAGTGGGTGTTGTTCTTGAAAAGTGTTGCTGTTTGTGATAAAGTTTGGGGCAAATGAGCCAAAGAATCAAGTCTTCAAAATAGCATATATCTTGTTGAGAGGCATCATTTATGAGCCCACTTGCTGTGTGGAATGGTATCTGCTTGGTTTATATCTCCCTTTCTTTGAAATCTTTCCCTTAGTGCTTTCCTATCAGACAAAGGCACAAAAATGACTGCATATTATAGCCATTCCACATCTTATGAATACATTCTAGTGAATCGGGCG

mRNA sequence

CTTGTTTCTTAATAAAAACAGAGCAACTGTCGGGCAGGGCAGGCGTTGCCAACTAAAATGCAAGGATGATTCGAAGAACAGTACAATGGAACCGTATTCCTTGTCGGGTATGACATAAATAACCGCATCTACAGCTTCAGCCCATCAAACGATCCAAATAGAAAAAACCCAAAATAGAAATGATCCAAACCGAAGTTGGAAGAGTTGTTTTCGAGAGAAGCGAGTAAGCAAAACCCCTTTTGTGATAAATCCCAAATCCATAAGCTTCTCGCCAAGCAACCAAGGAATCTACCGACTTCAATGGCTGTCGCTGGTCAGAAATCAGACCTCGATGGCAAGGTTTGGAGCCTTTGTAGTATACCCTTTTGGCAAAATGGCAATGTCTCCTCGTCTTCTGGTTCTTCGTCGACTGCTATTCATCGCGTTAATGAGCAAAGTAATGGTCATAAGCCAACAGAGAACTCGAGCTCCTATCCTTCTGGCAAGGTTTCGTTCATTGCGAAATCTCTACTTCCTACCAGAAGAAGGCTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATATGAACCTGGGAAGCAGGTAAAGAGTGCTATTGAGATACAGAATACGAGCAAGAATCATGTGGCTTTCAAATTTCAAACGACCGCGCCGAAGAGCTGCTACATGCGTCCTCCTGGCGGTATACTTGCTCCTGGTGAAAGTATCATCGCAACGGTGTTCAAGTTTGTGGAGCCACTAGAGAACAATGAGAAGCCTGTGGATCAAAAGAGGAGGGTTAAGTTTAAGATTGTGAGCTTGAAAGTGAAGGGTAACATGGACTATGTGCCAGAGCTGTTTGATGAACAAAGAGAACATGTAGCAGTTGAGCAAATACTGCAAGTTGTTTTTCTTGACCCGCAGCATTCTAATCCTGCTTTGGAGAAACTCTTCCGACAACTAGCTGTGGCCGAGGCTGCAACTGAGGTGCGTCAAAAGCCAGCAGAAGAAACAGACCCGAAAGTCGTTGGTGAAGGACTCGTCATCGACGAATGGAAAGAAAGAAGGGAAAGATACCTAGCTAAGCAACAGGTTGGAGGTGTGGATTTGGTGTGAAATCGAGCTGGTCATGTTATGAAAGTTGGAATCACATTCATGTGTGAACCTTTCTTTGAGGCATTCGTCGTCGAAGAGCAGTCATCAACATCGACATTAGCAATCAAAAGCGAATGGCTTAACGACTTGATTTGTGATGGCGTTTCATGGTCTTGTGTTTGTTTGATTGATTGGGTTGCTGTTTCTGTTGGAGTTTGAAGGTTTTGAGGTAAATAGTGGGTGTTGTTCTTGAAAAGTGTTGCTGTTTGTGATAAAGTTTGGGGCAAATGAGCCAAAGAATCAAGTCTTCAAAATAGCATATATCTTGTTGAGAGGCATCATTTATGAGCCCACTTGCTGTGTGGAATGGTATCTGCTTGGTTTATATCTCCCTTTCTTTGAAATCTTTCCCTTAGTGCTTTCCTATCAGACAAAGGCACAAAAATGACTGCATATTATAGCCATTCCACATCTTATGAATACATTCTAGTGAATCGGGCG

Coding sequence (CDS)

ATGGCTGTCGCTGGTCAGAAATCAGACCTCGATGGCAAGGTTTGGAGCCTTTGTAGTATACCCTTTTGGCAAAATGGCAATGTCTCCTCGTCTTCTGGTTCTTCGTCGACTGCTATTCATCGCGTTAATGAGCAAAGTAATGGTCATAAGCCAACAGAGAACTCGAGCTCCTATCCTTCTGGCAAGGTTTCGTTCATTGCGAAATCTCTACTTCCTACCAGAAGAAGGCTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATATGAACCTGGGAAGCAGGTAAAGAGTGCTATTGAGATACAGAATACGAGCAAGAATCATGTGGCTTTCAAATTTCAAACGACCGCGCCGAAGAGCTGCTACATGCGTCCTCCTGGCGGTATACTTGCTCCTGGTGAAAGTATCATCGCAACGGTGTTCAAGTTTGTGGAGCCACTAGAGAACAATGAGAAGCCTGTGGATCAAAAGAGGAGGGTTAAGTTTAAGATTGTGAGCTTGAAAGTGAAGGGTAACATGGACTATGTGCCAGAGCTGTTTGATGAACAAAGAGAACATGTAGCAGTTGAGCAAATACTGCAAGTTGTTTTTCTTGACCCGCAGCATTCTAATCCTGCTTTGGAGAAACTCTTCCGACAACTAGCTGTGGCCGAGGCTGCAACTGAGGTGCGTCAAAAGCCAGCAGAAGAAACAGACCCGAAAGTCGTTGGTGAAGGACTCGTCATCGACGAATGGAAAGAAAGAAGGGAAAGATACCTAGCTAAGCAACAGGTTGGAGGTGTGGATTTGGTGTGA

Protein sequence

MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRERYLAKQQVGGVDLV
Homology
BLAST of CmoCh02G001310 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 3.5e-90
Identity = 181/295 (61.36%), Postives = 213/295 (72.20%), Query Frame = 0

Query: 1   MAVAGQKSDLDGK-VWSLCSIPFWQNG---NVSSS---------SGSSSTAIHRVNEQSN 60
           M +  +K   DGK  W    IPF  +    N +SS         + SSST+ H  N   +
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 61  GHK------------------PTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPY 120
            H+                    +N    PS  VS +AKS LPT+RRL+LDPS KLYFPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 121 EPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLEN 180
           EPGKQV+SAI+I+NTSK+HVAFKFQTTAPKSC+MRPPG ILAPGE+IIATVFKFVEP EN
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

Query: 181 NEKPVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVVFLDPQHSNPALEK 240
           NEKP+DQ+ RVKFKI+SLKVKG MDYVPELFDEQ++ V+ EQIL+V+FLDP+ SNPALEK
Sbjct: 181 NEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEK 240

Query: 241 LFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRERYLAKQQVGGVDLV 265
           L RQLA A+AA E R+KP EET PK++GEGLVIDEWKERRERYLA+QQ  G D V
Sbjct: 241 LKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of CmoCh02G001310 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 4.6e-82
Identity = 164/252 (65.08%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 14  VWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQS-NGHKPTENSS-SYPSGKVSFIAKSLL 73
           ++ LC  PFWQ     S++ SSST     N +S +G++ T+ S+ S P   +S +A+SLL
Sbjct: 17  LFRLC--PFWQR---RSTTSSSSTQNPNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 76

Query: 74  PTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILA 133
           P RRRLRLDPS+ LYFPYEPGKQV+SAI+++NTSK+H AFKFQTTAPKSCYMRPPGG+LA
Sbjct: 77  PARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLA 136

Query: 134 PGESIIATVFKFVEPLENNEK-PVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVE 193
           PGES+ ATVFKFVE  ENNEK P++QK +VKFKI+SLKVK  ++YVPELFDEQ++ VAVE
Sbjct: 137 PGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVE 196

Query: 194 QILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRE 253
           Q+L+V+F+D    + ALEKL RQL  AEAA E R+KP  ET P+VVGEGLVIDEWKERRE
Sbjct: 197 QVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERRE 256

Query: 254 RYLAKQQVGGVD 263
           +YLA+QQV  VD
Sbjct: 257 KYLARQQVESVD 263

BLAST of CmoCh02G001310 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 3.7e-79
Identity = 163/289 (56.40%), Postives = 208/289 (71.97%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTA-----------------IHRVN 60
           MA+   KSD DG+ W    +PF +N N  + S SSS++                 IH+  
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPF-RNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSR 60

Query: 61  E---------QSNGHKPTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQ 120
                     +  G    +++++ PS  +S +A+SLLPT+RRL+LDPS KLYFPYEPGKQ
Sbjct: 61  HFQYHGPPVVEGLGQNHHQSAATIPS--MSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQ 120

Query: 121 VKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLENNEKPV 180
           V+SAI+I+NTSK+HVAFKFQTT PKSC+MRP G ILAPGE IIATVFKFVEP ENNEKP+
Sbjct: 121 VRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPM 180

Query: 181 DQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVVFLDPQHSNPALEKLFRQL 240
           +QK  VKFKI+SLK+K   DY+PELF+EQ++HV+ EQ+++VVFLDP++ N  +EKL  QL
Sbjct: 181 EQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQL 240

Query: 241 AVAEAATEVRQKPAEE-TDPKVVGEGLVIDEWKERRERYLAKQQVGGVD 263
           A A+AA E R+K +E    PK +GEGLVIDEWK+RRERYLA+QQ GGVD
Sbjct: 241 AEADAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGVD 285

BLAST of CmoCh02G001310 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.1e-06
Identity = 37/129 (28.68%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 84  KLYFPYEPGKQVKSAIEIQN-TSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFK 143
           +L FP+E  KQ   ++++ N T+   VAFK +TT P+   +RP  G++ PG+S   TV  
Sbjct: 13  ELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDSCNVTVTM 72

Query: 144 FVEPLENNEKPVDQKRRVKFKIVSLKVKG---NMDYVPELFDEQREHVAVEQILQVVFLD 203
             +     E P+D + + KF + ++ V     + + + E+F+++   V  +  L+VV++ 
Sbjct: 73  QAQ----KEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVVYIP 132

Query: 204 PQHSNPALE 209
               +P  E
Sbjct: 133 ANPPSPVPE 137

BLAST of CmoCh02G001310 vs. ExPASy Swiss-Prot
Match: Q9WV55 (Vesicle-associated membrane protein-associated protein A OS=Mus musculus OX=10090 GN=Vapa PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 3.2e-06
Identity = 36/128 (28.12%), Postives = 65/128 (50.78%), Query Frame = 0

Query: 77  LRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESI 136
           L LDP + L F       V + +++QN S   V FK +TTAP+   +RP  GI+ PG   
Sbjct: 15  LVLDPPSDLKFKGPFTDVVTTNLKLQNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGS-- 74

Query: 137 IATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVV 196
           I TV   ++P + +    ++K + KF + ++    N+  +  ++ E +    ++  L+ V
Sbjct: 75  IVTVSVMLQPFDYDP---NEKSKHKFMVQTIFAPPNISDMEAVWKEAKPDELMDSKLRCV 134

Query: 197 FLDPQHSN 205
           F  P  ++
Sbjct: 135 FEMPNEND 137

BLAST of CmoCh02G001310 vs. ExPASy TrEMBL
Match: A0A6J1G6M8 (vesicle-associated protein 4-2-like OS=Cucurbita moschata OX=3662 GN=LOC111451313 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.8e-145
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS
Sbjct: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. ExPASy TrEMBL
Match: A0A6J1I192 (vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.1e-142
Identity = 259/264 (98.11%), Postives = 260/264 (98.48%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVA QKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKP ENS SYPS
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVD KRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDHKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQR+HVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQRDHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. ExPASy TrEMBL
Match: A0A6J1CFB2 (vesicle-associated protein 4-2-like OS=Momordica charantia OX=3673 GN=LOC111011002 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.6e-123
Identity = 226/264 (85.61%), Postives = 241/264 (91.29%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAV  +KS  DGKVWS CSIPFWQNGN SSS+   ST++H V+ QSNG+K  E  SSY S
Sbjct: 1   MAVVDEKSGPDGKVWSFCSIPFWQNGNASSSASPPSTSVHNVHLQSNGNKSAEQPSSYSS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVS IAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEI+NTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSSIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIKNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEP ENNEKP+DQ+RRVKFKIVSLKVKG+MDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPPENNEKPLDQRRRVKFKIVSLKVKGDMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQ++ VAVEQILQVVFLDP++SNPALEKLFRQLA AEAATE RQ PAEET PK+VGEGL
Sbjct: 181 DEQKDQVAVEQILQVVFLDPENSNPALEKLFRQLAEAEAATEARQTPAEETGPKIVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. ExPASy TrEMBL
Match: A0A0A0KE43 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 4.6e-117
Identity = 220/267 (82.40%), Postives = 238/267 (89.14%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNV---SSSSGSSSTAIHRVNEQSNGHKPTENSSS 60
           MAVA +KS  DGKVWSLCSIPFWQN N    SSSS SSS+ IHRV+ QSNGHKP E+SSS
Sbjct: 1   MAVADEKSGPDGKVWSLCSIPFWQNSNAPSSSSSSSSSSSVIHRVHLQSNGHKPAEHSSS 60

Query: 61  YPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTA 120
           +P G VS IAKSL PT+RRLRLDPSN LYFP+EPGKQVKSAIEI+NTSKNHVAFKFQTTA
Sbjct: 61  HP-GVVSAIAKSLCPTKRRLRLDPSNNLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTA 120

Query: 121 PKSCYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVP 180
           PKSCYMRPPGGILAP ESIIATVFKFVEPL+NN+K VD KRRVKFKIVSLKVK ++DYVP
Sbjct: 121 PKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDLDYVP 180

Query: 181 ELFDEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVG 240
           ELFDEQ++ VAVEQILQ VFLDP HSNPALEK+ RQLA AEAA E+R KPAEETDPK+VG
Sbjct: 181 ELFDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVG 240

Query: 241 EGLVIDEWKERRERYLAKQQVGGVDLV 265
            GLV+DEWKERRERYLAKQQVGGVDLV
Sbjct: 241 GGLVLDEWKERRERYLAKQQVGGVDLV 266

BLAST of CmoCh02G001310 vs. ExPASy TrEMBL
Match: A0A5D3BT76 (Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold388G00900 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 5.1e-116
Identity = 219/266 (82.33%), Postives = 236/266 (88.72%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNV--SSSSGSSSTAIHRVNEQSNGHKPTENSSSY 60
           MAVA +KS  DGKVW+LCSIPFWQN N   SSSS SSS+ IHRV+ Q NGHKP E+SSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PSGKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAP 120
           P G VS IAKSL PT+RRLRLDPSNKLYFP+EPGKQVKSAIEI+NTSKNHVAFKFQTTAP
Sbjct: 61  P-GVVSAIAKSLRPTKRRLRLDPSNKLYFPFEPGKQVKSAIEIKNTSKNHVAFKFQTTAP 120

Query: 121 KSCYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPE 180
           KSCYMRPPGGILAP ES IATVFKFVEPL+NN+K VD KRRVKFKIVSLKVK +MDYVPE
Sbjct: 121 KSCYMRPPGGILAPDESTIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKSDMDYVPE 180

Query: 181 LFDEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGE 240
           LFDEQ++ VAVEQILQ VFLDP HSNPALEK+ RQLA AEAA E+R KPAEETDPK+VG 
Sbjct: 181 LFDEQKDRVAVEQILQAVFLDPDHSNPALEKVCRQLAEAEAAAEMRHKPAEETDPKIVGG 240

Query: 241 GLVIDEWKERRERYLAKQQVGGVDLV 265
           GLV+DEWKERRERYLAKQQVGGVDLV
Sbjct: 241 GLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of CmoCh02G001310 vs. NCBI nr
Match: XP_022947458.1 (vesicle-associated protein 4-2-like [Cucurbita moschata])

HSP 1 Score: 525.0 bits (1351), Expect = 3.7e-145
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS
Sbjct: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. NCBI nr
Match: KAG7034938.1 (Vesicle-associated protein 4-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 523.5 bits (1347), Expect = 1.1e-144
Identity = 263/264 (99.62%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENS SYPS
Sbjct: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSGSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. NCBI nr
Match: XP_023532461.1 (vesicle-associated protein 4-2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 261/264 (98.86%), Postives = 262/264 (99.24%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVA QKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENS SYPS
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSGSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQR+HVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQRDHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. NCBI nr
Match: XP_022970681.1 (vesicle-associated protein 4-2-like [Cucurbita maxima])

HSP 1 Score: 515.8 bits (1327), Expect = 2.2e-142
Identity = 259/264 (98.11%), Postives = 260/264 (98.48%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVA QKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKP ENS SYPS
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVD KRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDHKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQR+HVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQRDHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGVDLV 265
           VIDEWKERRERYLAKQQVGGVDLV
Sbjct: 241 VIDEWKERRERYLAKQQVGGVDLV 264

BLAST of CmoCh02G001310 vs. NCBI nr
Match: KAG6604822.1 (Vesicle-associated protein 4-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 514.2 bits (1323), Expect = 6.5e-142
Identity = 258/261 (98.85%), Postives = 259/261 (99.23%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSSSYPS 60
           MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENS SYPS
Sbjct: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPTENSGSYPS 60

Query: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120
           GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKS 120

Query: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180
           CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF
Sbjct: 121 CYMRPPGGILAPGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELF 180

Query: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240
           DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL
Sbjct: 181 DEQREHVAVEQILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGL 240

Query: 241 VIDEWKERRERYLAKQQVGGV 262
           VIDEWKERRERYLAKQQVG +
Sbjct: 241 VIDEWKERRERYLAKQQVGAL 261

BLAST of CmoCh02G001310 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 2.5e-91
Identity = 181/295 (61.36%), Postives = 213/295 (72.20%), Query Frame = 0

Query: 1   MAVAGQKSDLDGK-VWSLCSIPFWQNG---NVSSS---------SGSSSTAIHRVNEQSN 60
           M +  +K   DGK  W    IPF  +    N +SS         + SSST+ H  N   +
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 61  GHK------------------PTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPY 120
            H+                    +N    PS  VS +AKS LPT+RRL+LDPS KLYFPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 121 EPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLEN 180
           EPGKQV+SAI+I+NTSK+HVAFKFQTTAPKSC+MRPPG ILAPGE+IIATVFKFVEP EN
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

Query: 181 NEKPVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVVFLDPQHSNPALEK 240
           NEKP+DQ+ RVKFKI+SLKVKG MDYVPELFDEQ++ V+ EQIL+V+FLDP+ SNPALEK
Sbjct: 181 NEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSNPALEK 240

Query: 241 LFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRERYLAKQQVGGVDLV 265
           L RQLA A+AA E R+KP EET PK++GEGLVIDEWKERRERYLA+QQ  G D V
Sbjct: 241 LKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADSV 295

BLAST of CmoCh02G001310 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 2.9e-87
Identity = 180/314 (57.32%), Postives = 213/314 (67.83%), Query Frame = 0

Query: 1   MAVAGQKSDLDGK-VWSLCSIPFWQNG---NVSSS---------SGSSSTAIHRVNEQSN 60
           M +  +K   DGK  W    IPF  +    N +SS         + SSST+ H  N   +
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 61  GHK------------------PTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPY 120
            H+                    +N    PS  VS +AKS LPT+RRL+LDPS KLYFP+
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPW 120

Query: 121 -------------------EPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGIL 180
                              EPGKQV+SAI+I+NTSK+HVAFKFQTTAPKSC+MRPPG IL
Sbjct: 121 FVSEDLRSIDTSLKFLSFNEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAIL 180

Query: 181 APGESIIATVFKFVEPLENNEKPVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVE 240
           APGE+IIATVFKFVEP ENNEKP+DQ+ RVKFKI+SLKVKG MDYVPELFDEQ++ V+ E
Sbjct: 181 APGETIIATVFKFVEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKE 240

Query: 241 QILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRE 265
           QIL+V+FLDP+ SNPALEKL RQLA A+AA E R+KP EET PK++GEGLVIDEWKERRE
Sbjct: 241 QILRVIFLDPERSNPALEKLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRE 300

BLAST of CmoCh02G001310 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 305.8 bits (782), Expect = 3.3e-83
Identity = 164/252 (65.08%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 14  VWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQS-NGHKPTENSS-SYPSGKVSFIAKSLL 73
           ++ LC  PFWQ     S++ SSST     N +S +G++ T+ S+ S P   +S +A+SLL
Sbjct: 17  LFRLC--PFWQR---RSTTSSSSTQNPNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 76

Query: 74  PTRRRLRLDPSNKLYFPYEPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILA 133
           P RRRLRLDPS+ LYFPYEPGKQV+SAI+++NTSK+H AFKFQTTAPKSCYMRPPGG+LA
Sbjct: 77  PARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLA 136

Query: 134 PGESIIATVFKFVEPLENNEK-PVDQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVE 193
           PGES+ ATVFKFVE  ENNEK P++QK +VKFKI+SLKVK  ++YVPELFDEQ++ VAVE
Sbjct: 137 PGESVFATVFKFVEHPENNEKQPLNQKSKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVE 196

Query: 194 QILQVVFLDPQHSNPALEKLFRQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRE 253
           Q+L+V+F+D    + ALEKL RQL  AEAA E R+KP  ET P+VVGEGLVIDEWKERRE
Sbjct: 197 QVLRVIFIDADRPSAALEKLKRQLDEAEAAVEARKKPPPETGPRVVGEGLVIDEWKERRE 256

Query: 254 RYLAKQQVGGVD 263
           +YLA+QQV  VD
Sbjct: 257 KYLARQQVESVD 263

BLAST of CmoCh02G001310 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 2.6e-80
Identity = 163/289 (56.40%), Postives = 208/289 (71.97%), Query Frame = 0

Query: 1   MAVAGQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTA-----------------IHRVN 60
           MA+   KSD DG+ W    +PF +N N  + S SSS++                 IH+  
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPF-RNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSR 60

Query: 61  E---------QSNGHKPTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPYEPGKQ 120
                     +  G    +++++ PS  +S +A+SLLPT+RRL+LDPS KLYFPYEPGKQ
Sbjct: 61  HFQYHGPPVVEGLGQNHHQSAATIPS--MSSVARSLLPTKRRLKLDPSAKLYFPYEPGKQ 120

Query: 121 VKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLENNEKPV 180
           V+SAI+I+NTSK+HVAFKFQTT PKSC+MRP G ILAPGE IIATVFKFVEP ENNEKP+
Sbjct: 121 VRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPGEEIIATVFKFVEPPENNEKPM 180

Query: 181 DQKRRVKFKIVSLKVKGNMDYVPELFDEQREHVAVEQILQVVFLDPQHSNPALEKLFRQL 240
           +QK  VKFKI+SLK+K   DY+PELF+EQ++HV+ EQ+++VVFLDP++ N  +EKL  QL
Sbjct: 181 EQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVSEEQVMRVVFLDPENPNSMMEKLKSQL 240

Query: 241 AVAEAATEVRQKPAEE-TDPKVVGEGLVIDEWKERRERYLAKQQVGGVD 263
           A A+AA E R+K +E    PK +GEGLVIDEWK+RRERYLA+QQ GGVD
Sbjct: 241 AEADAADEARKKASEGIVGPKPIGEGLVIDEWKQRRERYLAQQQ-GGVD 285

BLAST of CmoCh02G001310 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 213.8 bits (543), Expect = 1.7e-55
Identity = 120/210 (57.14%), Postives = 141/210 (67.14%), Query Frame = 0

Query: 1   MAVAGQKSDLDGK-VWSLCSIPFWQNG---NVSSS---------SGSSSTAIHRVNEQSN 60
           M +  +K   DGK  W    IPF  +    N +SS         + SSST+ H  N   +
Sbjct: 1   MTMTEEKPTSDGKGGWGFFKIPFRNSSGHRNAASSAATSPFPSGASSSSTSSHLHNHHQH 60

Query: 61  GHK------------------PTENSSSYPSGKVSFIAKSLLPTRRRLRLDPSNKLYFPY 120
            H+                    +N    PS  VS +AKS LPT+RRL+LDPS KLYFPY
Sbjct: 61  HHQHHHQHHHQLGYNGPHGDGSGQNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPY 120

Query: 121 EPGKQVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLEN 180
           EPGKQV+SAI+I+NTSK+HVAFKFQTTAPKSC+MRPPG ILAPGE+IIATVFKFVEP EN
Sbjct: 121 EPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKFVEPPEN 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYN23.5e-9061.36Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q1ECE04.6e-8265.08Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q8LPQ73.7e-7956.40Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q84WW51.1e-0628.68Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9WV553.2e-0628.13Vesicle-associated membrane protein-associated protein A OS=Mus musculus OX=1009... [more]
Match NameE-valueIdentityDescription
A0A6J1G6M81.8e-145100.00vesicle-associated protein 4-2-like OS=Cucurbita moschata OX=3662 GN=LOC11145131... [more]
A0A6J1I1921.1e-14298.11vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 ... [more]
A0A6J1CFB25.6e-12385.61vesicle-associated protein 4-2-like OS=Momordica charantia OX=3673 GN=LOC1110110... [more]
A0A0A0KE434.6e-11782.40MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV... [more]
A0A5D3BT765.1e-11682.33Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
XP_022947458.13.7e-145100.00vesicle-associated protein 4-2-like [Cucurbita moschata][more]
KAG7034938.11.1e-14499.62Vesicle-associated protein 4-2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023532461.11.6e-14398.86vesicle-associated protein 4-2-like [Cucurbita pepo subsp. pepo][more]
XP_022970681.12.2e-14298.11vesicle-associated protein 4-2-like [Cucurbita maxima][more]
KAG6604822.16.5e-14298.85Vesicle-associated protein 4-2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G21450.12.5e-9161.36PapD-like superfamily protein [more]
AT4G21450.32.9e-8757.32PapD-like superfamily protein [more]
AT5G54110.13.3e-8365.08membrane-associated mannitol-induced [more]
AT4G05060.12.6e-8056.40PapD-like superfamily protein [more]
AT4G21450.21.7e-5557.14PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 77..187
e-value: 2.3E-29
score: 101.4
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 76..198
score: 22.187311
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 72..202
e-value: 8.9E-29
score: 102.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..60
NoneNo IPR availablePANTHERPTHR10809:SF101VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 38..261
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 38..261
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 66..206

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh02G001310.1CmoCh02G001310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane